CN105624056B - The production method of one plant of plant growth-promoting bacteria for being isolated from phyllosphere and its microbial inoculum - Google Patents
The production method of one plant of plant growth-promoting bacteria for being isolated from phyllosphere and its microbial inoculum Download PDFInfo
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- CN105624056B CN105624056B CN201410636383.1A CN201410636383A CN105624056B CN 105624056 B CN105624056 B CN 105624056B CN 201410636383 A CN201410636383 A CN 201410636383A CN 105624056 B CN105624056 B CN 105624056B
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Abstract
The present invention relates to a plant heights to imitate plant growth-promoting bacteria --- the Low-cost production method and application method of bacillus subtilis and its microbial inoculum, belongs to microbial manure and energy-conserving and emission-cutting technology field.The bacterial strain that the present invention uses, which is separate to come from pine tree blade one plant, has bacillus subtilis (Bacillus subtilis) the SZX11 bacterial strains for promoting plant growth function, the bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is：CGMCC No.8188.The present invention prepares agricultural microbial agent using Sweet Potato Starch Processing Waste Water as liquid state fermentation culture medium, large-scale culture bacillus subtilis, the process energy consumption is low, low production cost, it is easy to operate, Sweet Potato Starch Processing Waste Water can also be made to get utilization, reduce disposal of pollutants.Microbial inoculum produced by the invention is used for the production of tealeaves and vegetables, can not only significantly improve yield, but also can be obviously improved the quality of agricultural product, show wide application prospect.
Description
Technical field：
The high-efficiency plant Promoting bacteria (bacillus subtilis SZX11 bacterial strains) detached from pine tree blade the present invention relates to one plant,
And its Low-cost production method of microbial inoculum, belong to microbial manure and energy-conserving and emission-cutting technology field.
Background technology：
Bacillus subtilis (Bacillus subtilis) be a kind of aerobic, the degeneration-resistant spore of interior life bacillus, greatly
Small (0.8~1.2) μ m (1.5~4.0) μm, Gram-positive can produce pod membrane, and raw in being given birth in gemma or being close, bacterium colony is thick
Rough, can liquefy gelatin, peptonized milk, restore nitrate, hydrolyze starch, and soil, lake, sea are widely present in for typical aerobic bacteria
The body surface in ocean and animals and plants, itself is not pathogenic.Bacillus subtilis can be by successfully colonizing in plant rhizosphere, body surface or body
It is interior, inhibit growth of pathogenic bacteria with the nutrition around pathogen competitive plant, secretion antibacterial material, while inducing plant defense system
Pathogen invasion is resisted, to have the effect of biological and ecological methods to prevent plant disease, pests, and erosion.Bacillus subtilis can also generate similar cell mitogen, plant life
The substance of long hormone promotes the growth of plant.Such as：AgraQuest companies of the U.S. are opened with bacillus subtilis QST713 bacterial strains
Active bacteria formulation fungicide SerenadeTM is sent out, and in the registration by U.S.EPA (EPA) in 2000, it is a variety of for preventing
The diseases such as powdery mildew, frost and dew disease, epidemic disease, the gray mold of crop.MicroBios of the bacillus subtilis MBI600 in Britain
Group Ltd., Japanese IdemistuKosanCo., Ltd. etc. has been registered, and carries out industrialization production, is used
In prevention leaf diseases (gray mold, powdery mildew) and root disease (droop, root rot, black spot etc.).Bacillus subtilis
FZB24 is registered in countries such as Germany, the U.S., and is put into production in German Bayer companies, for preventing Phytophthora infestans
Disease, gray mold and wheat powdery mildew are alternatively arranged as volume increase accelerating agent.As that studies bacillus subtilis deepens continuously, hair
Existing its has in industry, agricultural, medical and health, health care of food, aquaculture etc. extensively using value.
Chinese invention patent (application number：201210328716.5) in disclose a bacillus subtilis T-500 bacterial strains, protect
It is CGMCC No.6058 to hide number, to rice rice blast fungus, Rhizoctonia solani, rice leaf spot bacteria, Ustilaginoidea virens, water
The pathogens such as rice Xanthomonas campestris PV.oryzicola, Fusarium graminearum, aspergillus flavus and black-koji mould show stronger bacteriostatic activity, and
There are preferable prevention and control to act on its caused disease, fermentation medium composition includes：Carbon source (sucrose or starch), nitrogen source (soya bean
The mixing of one or more of powder, beancake powder, corn paddle kind), (beef leaches one in cream, yeast extract or fish meal trace element
Kind or two kinds of mixing).Chinese invention patent (application number：201210229066.9) in disclose one plant it is withered with bacteriostatic activity
Careless bacillus CX31 bacterial strains, deposit number are CCTCC No.M2012138, and the culture of the bacterial strain is trained using beef extract-peptone
Support base.Chinese invention patent (application number：201110205587.6) in disclose one plant of endophytic Bacillus subtilis, deposit number
For CGMCC No.4844, have good control effect to pepper seasonal febrile diseases, cultivate the culture medium that the bacterial strain uses for：PDA is cultivated
Base.
Chinese invention patent (application number：201210215134.6) in disclose a bacillus subtilis RB bacterial strains, protect
It is CGMCC No.6268 to hide number, and discloses application of the bacterial strain in processing sludge, fowl and animal excrement or crop straw, should
The culture medium of bacterial strain is prepared using cornstarch, brown sugar, wheat bran, fish meal, sucrose and inorganic salts.Chinese invention patent (application
Number：201210127519.7) bacillus subtilis is disclosed in) KW119 bacterial strains, deposit number is CGMCC No.5789,
Production protease ability is higher under habitat conditions in chicken intestinal for it, and fermentation medium is mainly using dregs of beans as substrate.Chinese invention
Patent (application number：201110443948.0) a bacillus subtilis CH-001 bacterial strains, deposit number CCTCC are disclosed in
No.M2011111, it has antagonism to pathogenic bacteria such as staphylococcus aureuses, has probiotic properties, is suitable for animal egg
White feed addictive, culture medium main component are feather meal and inorganic salts.Chinese invention patent (application number：
201010129783.5) a bacillus subtilis ANSB060 bacterial strains are disclosed in, deposit number is CGMCC No.3440, and
Its application in animal feed is disclosed, culture medium main component is peptone, glucose and inorganic salts.Chinese invention is special
Sharp (application number：200410101021.9) a bacillus subtilis B115 bacterial strains, deposit number CGMCC are disclosed in
No.1210, and its method for being used to prepare fishing Tiny ecosystem improver of water quality is disclosed, culture medium main component is soya-bean cake
Powder, wheat bran and corn flour.
In conclusion with promoting the bacterial strain of the bacillus subtilis of plant growth and antagonism plant disease more, it is used for
The bacterial strain of animal feed is also relatively common, and cultural method is also different, still, has no the bacterial strain for being isolated from plant phyllospheric,
Also the report using high concentration Sweet Potato Starch Processing Waste Water culture bacillus subtilis is had no.
China is that maximum sweet potato producing country, total output are stablized at 100,000,000 tons or more in the world, and it is left to account for global 80%
It is right.In the crops main product in the whole nation, sweet potato is only second to rice, wheat and corn, occupies the 4th.Sweet potato plants less investment, output
More, the edible dry matter of unit area occupies first of various crops.Sweet potato strength of combatting natural disaster is strong, drought-enduring, and impoverishment tolerant, Hills also can
Plantation can also obtain preferable yield in the place of the more difficult growth of other crops.Sweet potato is perishable, should not store for a long time.Sweet potato
Deep processing, can solve because store fresh sweet potato it is improper due to lead to a large amount of rotten potatos the phenomenon that, sweet potato purified starch passes through different depth
Processing, can produce hundreds of value products, rise in value 10-30 times or so.But current processing technology, often produce 1
Ton sweet potato starch about 10 tons or so high concentrated organic wastewaters of output, organic matter contains in the sweet potato cell liquid wherein after starch separation
Highest is measured, COD mainly contains dissolubility starch, protein, pectin, organic acid and a small amount of oil up to 20000mg/L or more
Fat is dealt with improperly, easily putrefactive fermentation, so that water quality is blacked smelly.And use general sewage treatment process by such high concentration
Wastewater treatment qualified discharge, energy consumption are very big.Consider from another angle, organic matter in Sweet Potato Starch Processing Waste Water (including：
Protein, pectin, carbohydrate etc.) it is the good nutrition substrate that microorganism grows, microbiological culture media can be made and carry out recycling
It utilizes.
Invention content：
The object of the present invention is to provide one plant of antagonistic phytopathogen, promote the bacillus subtilis of plant growth, and carries
For it is a kind of being capable of the recycling high concentration Sweet Potato Starch Processing Waste Water method that produces its microbial inoculum.
Bacterial strain used in the present invention be by inventor from separation screening on the blade of pine tree come out one plant have promote
Bacillus subtilis (Bacillus subtilis) SZX11 bacterial strains of plant growth, antagonistic phytopathogen function, the bacterial strain
It was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center in 16th in September in 2013, deposit number is：
CGMCC No.8188, depositary institution address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode：100101.
The production method of bacillus subtilis agricultural microbial agent of the present invention is as follows：
1, Sweet Potato Starch Processing Waste Water pre-processes：In high concentration Sweet Potato Starch Processing Waste Water 0.1%~1% is added according to weight ratio
Plant ash (comes from the solid-state castoff of biomass thermal power plant, main component is potassium carbonate), repeats to be uniformly mixed, then use stone
Buck adjusted the pH to 7.0~8.5 of waste water, in 95~110 DEG C of microwave heatings 3~15 minutes.
2, prepared by liquid seeds：The withered of slant preservation is inoculated with into the triangular flask equipped with the Sweet Potato Starch Processing Waste Water pre-processed
Careless bacillus, in 45~55 DEG C, 120~200 revs/min of shaken cultivations 24~36 hours obtain liquid seeds.
3, liquid state fermentation：To equipped in the Sweet Potato Starch Processing Waste Water fermentation tank pre-processed be inoculated with volume ratio be 2%~15%
Liquid seeds lead to filtrated air culture 12~30 hours in 35~50 DEG C；Cultured zymotic fluid through count plate,
Viable count reaches 2 × 109CFU/ milliliters or more, gained zymotic fluid can directly be used as liquid bacterial agent.
4, prepared by solid fungicide：To cross biological carbon (remaining solid matter, the main component after biomass pyrolytic of 30 mesh sieve
It is carbon) it is carrier, it is fully mixed according to zymotic fluid: zymotic fluid is added in biological carbon by the weight ratio of biological carbon 1: 1~1: 3
It closes uniformly, obtains solid-state microbial inoculum, at 55~60 DEG C, aeration-drying to microbial inoculum water content is wrapped by weight being 15%~25%
Dress is stored at shady and cool drying, spare.
The application method of microorganism Bacillus subtilis microbial inoculum of the present invention is as follows：
1, it is 3 × 10 zymotic fluid or solid fungicide to be diluted to viable count with irrigation water6CFU/ milliliters~3 × 107CFU/
Milliliter is uniformly mixed, and is uniformly sprayed to the whole strain of crop according to the dosage of 0.3~3 cubic metre of dilution per acre or ditch applies crop
In soil near root；
2, zymotic fluid or solid fungicide are added in organic fertilizer, are uniformly mixed, the bacillus subtilis in organic fertilizer is made to live
Bacterium number is 2 × 107CFU/ grams~2 × 108CFU/ grams, in being manured into soil as base manure according to 1~3 ton per acre of dosage.
Specific implementation mode：
Bacillus subtilis provided by the present invention can promote absorption and utilization of the crop to nutrient, and can effectively prevent
Plurality of plant diseases is controlled, the microbial bacterial agent of technical solution production provided by the invention, which is applied to agricultural production, can reduce chemical fertilizer
With the usage amount of pesticide, so as to improve farm environment quality；And production cost is very low, and high-concentration waste water is made to obtain recycling profit
With reduction pollutant emission.Specific implementation mode is as follows：
Embodiment 1：Sweet Potato Starch Processing Waste Water produces microorganism Bacillus subtilis microbial inoculum
(1) Sweet Potato Starch Processing Waste Water pre-processes：According to the vegetation of weight ratio addition 0.5% in high concentration Sweet Potato Starch Processing Waste Water
Ash (comes from the solid-state castoff of biomass thermal power plant, main component is potassium carbonate), repeats to be uniformly mixed, then use limewash
The pH to 8.0 for adjusting waste water, in 100 DEG C of microwave heatings 5 minutes.
(2) prepared by liquid seeds：Slant preservation is inoculated with into the triangular flask equipped with the Sweet Potato Starch Processing Waste Water pre-processed
Bacillus subtilis, in 50 DEG C, 150 revs/min of shaken cultivations 28 hours obtain liquid seeds.
3, liquid state fermentation：To equipped with the liquid that inoculation volume ratio is 5% in the Sweet Potato Starch Processing Waste Water fermentation tank pre-processed
Seed leads to filtrated air culture 16 hours in 45 DEG C；Cultured zymotic fluid through count plate, viable count reaches 2 ×
109CFU/ milliliters or more, gained zymotic fluid can directly be used as liquid bacterial agent.
4, prepared by solid fungicide：To cross biological carbon (remaining solid matter, the main component after biomass pyrolytic of 30 mesh sieve
It is carbon) it is carrier, according to zymotic fluid: zymotic fluid is added in biological carbon by the weight ratio of biological carbon 1: 2, it is sufficiently mixed
It is even, solid-state microbial inoculum is obtained, at 55~60 DEG C, by weight being 15%~25%, packaging is deposited for aeration-drying to microbial inoculum water content
It is placed at shady and cool drying, it is spare.
Embodiment 2：Microorganism Bacillus subtilis microbial inoculum is produced applied to organic tea
In uniformly experimental field 2 mu or so of organic tea garden selection tea tree growing way, experiment tea tree manages with other tea trees of base
It keeps always, only applying fertilizer different.5 pieces are experimental field divided into, 5 processing is corresponded to respectively, is set between each processing
Microbial inoculum is diluted with tap water or zymotic fluid is directly diluted use by the isolation strip for having 2 meters or so, and 5 processing are respectively clear water
Compare (CK), starch wastewater control (FCK), high concentration (2 × 107CFU/ milliliters) spray (H), middle concentration (1 × 107CFU/ millis
Rise) spray (M), low concentration (5 × 106CFU/ milliliters) (L) is sprayed, it is used according to the amount of 2 liters every square metre of microbial inoculum dilutions.
3 microbial inoculums are applied in each processing in winter in an identical manner, and spring harvests tealeaves, the results showed that：Using pair of wastewater dilution liquid
Compare no significant difference with clear water according to spring tea yield, and dramatically increased using the spring tea yield of microbial inoculum, wherein in application concentration and
The spring tea yield of high concentration microbial inoculum is suitable, all than 35% or more clear water control volume increase, using the spring tea yield of low concentration microbial inoculum
Than clear water control volume increase 20% or so.And the Tea Polyphenols in Tea and amino acid content of application microbial inoculum are significantly higher than clear water pair
According to.
Embodiment 3：Organic fertilizer rich in bacillus subtilis is applied to Tea Production
One piece of tea tree growing way is selected in tea place uniformly experimental field, is evenly dividing as 6 cells, and each cell about 200 is flat
Square rice, be respectively set to not apply fertilizer control, application of organic fertilizers and application are rich in bacillus subtilis (6 × 107CFU/ grams) have
The 3 kinds of processing of machine fertilizer, each processing 2 is parallel, is designed with 2 meters or so of isolation strip between each processing, organic fertilizer and is rich in withered grass
The organic fertilizer of bacillus is according to 1 ton per acre of dosage, ditch spread 1 time before entering the winter, and spring harvests tealeaves, the results showed that：Using
Organic fertilizer spring tea yield increased volume increase 15% or so, and apply biological organic fertilizer spring tea yield increased volume increase 35% with
On, and the content of water extraction, tea polyphenols and amino acid is significantly higher than control in the tealeaves of application biological organic fertilizer.
Embodiment 4：Microorganism Bacillus subtilis microbial inoculum is applied to Rape-seed production
It is tested using green house of vegetables cell, 8 identical hillslope processes of condition, each cell 100m is set2, 2
Cell is 1 group of parallel test, is divided into 4 groups, plants rape, in the Brassica Napus Seedling phase, the 1st group of 2 cells are not applied fertilizer and compared；The
2 groups of 2 cells respectively sprayed after rape complete germination and after 3 weeks according to 100 kgs/acre of dosage microbial inoculum dilution (2 ×
107CFU/ milliliters)；3rd group of 2 cells according to 15 kgs/acre of dose, respectively urinate after rape complete germination and after 3 weeks by application
It is plain primary；4th group 2 are respectively primary using urea after rape complete germination and after 3 weeks according to 15 kgs/acre of dose, and
A microbial inoculum dilution (2 × 10 is sprayed after applying urea every time according to 100 kgs/acre within 1 day7CFU/ milliliters), each cell phase
Same irrigation and way to manage.Growth of rape investigates yield and quality after 5 weeks.Microbial inoculum is administered alone and the oil of urea is administered alone
Dish yield is almost the same, all the check plot volume increase 50% or so than not applying fertilizer；The cell that two kinds of fertilizer are all applied is smaller than compareing
85% or more area's volume increase, and the content of NO3-N and NO2-N is substantially less than the cell that urea is administered alone in rape.
Claims (3)
1. a kind of production method of bacillus subtilis agricultural microbial agent, includes the following steps：
(1) Sweet Potato Starch Processing Waste Water pre-processes：According to the grass of weight ratio addition 0.1%~1% in high concentration Sweet Potato Starch Processing Waste Water
Wood ash repeats to be uniformly mixed, then adjusts the pH to 7.0~8.5 of waste water with limewash, in 95~110 DEG C of 3~15 points of microwave heatings
Clock；The plant ash comes from the solid-state castoff of biomass thermal power plant, and main component is potassium carbonate；
(2) prepared by liquid seeds：The withered grass of slant preservation is inoculated with into the triangular flask equipped with the Sweet Potato Starch Processing Waste Water pre-processed
Bacillus, in 45~55 DEG C, 120~200 revs/min of shaken cultivations 24~36 hours obtain liquid seeds；
(3) liquid state fermentation：To equipped with the liquid that inoculation volume ratio is 2%~15% in the Sweet Potato Starch Processing Waste Water fermentation tank pre-processed
Body seed leads to filtrated air culture 12~30 hours in 35~50 DEG C；Cultured zymotic fluid is through count plate, viable bacteria
Number reaches 2 × 109CFU/ milliliters or more, gained zymotic fluid can directly be used as liquid bacterial agent；
(4) prepared by solid fungicide：Using the biological carbon of the sieve of 30 mesh excessively as carrier, according to zymotic fluid：Biological carbon is 1：1~1：3 weight
Ratio is measured, zymotic fluid is added in biological carbon, is sufficiently mixed uniformly, obtains solid-state microbial inoculum, at 55~60 DEG C, aeration-drying is extremely
By weight being 15%~25%, packaging is stored at shady and cool drying microbial inoculum water content, spare；The biological carbon is biomass
Remaining solid matter after pyrolysis, main component are carbon；
Wherein, the bacillus subtilis is with the bacillus subtilis for promoting plant growth, antagonistic phytopathogen function
(Bacillus subtilis) SZX11 bacterial strains, the bacterial strain are preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life
Object center, deposit number：CGMCC No.8188.
2. the application method of agricultural microbial agent described in claim 1, it is characterised in that：Zymotic fluid or solid fungicide are used
It is 3 × 10 that irrigation water, which is diluted to viable count,6CFU/ milliliters~3 × 107It CFU/ milliliters, is uniformly mixed, it is vertical according to per acre 0.3~3
The dosage of square rice dilution to the whole strain of crop uniformly spray or ditch apply crop root near soil in.
3. the application method of agricultural microbial agent described in claim 1, it is characterised in that：Zymotic fluid or solid fungicide are added
Enter in organic fertilizer, be uniformly mixed, it is 2 × 10 to make the bacillus subtilis viable count in organic fertilizer7CFU/ grams~2 × 108CFU/
Gram, in being manured into soil as base manure according to 1~3 ton per acre of dosage.
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| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN107840706A (en) * | 2017-10-17 | 2018-03-27 | 中国科学院生态环境研究中心 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
| CN112998032B (en) * | 2021-03-02 | 2021-12-17 | 广东省科学院生物工程研究所 | Crop leaf surface spraying type bacillus inoculant and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
| CN105087410A (en) * | 2014-05-11 | 2015-11-25 | 无锡中科活力生物技术有限公司 | Novel aquaculture probiotics, and production method and use thereof |
Family Cites Families (1)
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
| CN105087410A (en) * | 2014-05-11 | 2015-11-25 | 无锡中科活力生物技术有限公司 | Novel aquaculture probiotics, and production method and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100;Xiaojia Hu等;《Appl Microbiol Biotechnol》;20050303;第68卷;第802-807页 * |
| 枯草芽孢杆菌B931防治植物病害和促进植物生长的作用;张霞等;《作物学报》;20071231;第33卷(第2期);第236-241页 * |
| 枯草芽孢杆菌对茶树的增产效果试验;雷该翔等;《茶叶通讯》;20121231;第39卷(第4期);第26-28页，尤其是第26页摘要，第28页左栏第4段，表2-4 * |
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