CN102924156A - Culture medium and cultural method for cultivating coprinus comatus - Google Patents
Culture medium and cultural method for cultivating coprinus comatus Download PDFInfo
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- CN102924156A CN102924156A CN2012104795672A CN201210479567A CN102924156A CN 102924156 A CN102924156 A CN 102924156A CN 2012104795672 A CN2012104795672 A CN 2012104795672A CN 201210479567 A CN201210479567 A CN 201210479567A CN 102924156 A CN102924156 A CN 102924156A
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Abstract
The invention provides a culture medium for cultivating coprinus comatus, which is composed of chestnut shells, crop straws or corncobs, dry livestock manure, calcium superphosphate, quick lime, enzyme bacterium and water. The method utilizing the culture medium to cultivate the coprinus comatus comprises the following steps: first, uniformly stirring all raw materials to produce a base material; second, stacking the base material for fermentation; third, bagging the fermented base material for inoculation so as to produce bacteria sticks; fourth, placing the bacteria sticks in a bacteria cultivating room; fifth, debagging the bacteria sticks so as to allow the bacteria sticks to be stood in a bed and is covered with soil; sixth, carrying out water spray management; and seventh, picking the coprinus comatus. According to the invention, the chestnut shells are basic material of the culture medium which is green and pollution-free, the cultivating process is simple, and the production cost is low; and the coprinus comatus is high in yield, good in quality, and high in biotransformation efficiency.
Description
Technical field
The invention belongs to the artificial cultivation technique field of edible mushrooms, be specifically related to a kind of substratum and cultivating method thereof of cultivating drumstick mushroom.
Background technology
Along with the development of food-processing industry and improving constantly of living standards of the people, Chinese chestnut food more and more receives people's concern and likes, the Chinese chestnut product innovation emerges in an endless stream, and directly causes the quantity of the waste-chestnut shell after Chinese chestnut is processed sharply to increase.
China's Chinese chestnut resource is extremely abundant, Chinese chestnut output reaches 60% of Gross World Product, the chestnut shell of annual residue flood tide, but these chestnut shells are not reasonably utilized and are developed, major part is used as fuel wood and burns, or is dropped the one side of banking up, and gives free rein to rot, the serious waste that not only causes resource, the destruction of also having caused environment.
In recent years, pay attention to gradually the research and development to the chestnut shell comprehensive utilization both at home and abroad, but mostly concentrate on the aspects such as solid shape carbon production, tannin extract and natural pigment extraction, and less for the report of edible fungus culturing aspect.Find after deliberation, contain the compositions such as abundant lignocellulose, organic acid, polysaccharide, protein, VITAMIN in the chestnut shell, can provide edible fungi growth required nutritive substance, therefore utilize the chestnut shell culturing edible fungus, can fully excavate social value and the economic worth of chestnut shell.
" hubei agricultural science " the 3rd phase in 2010 discloses the method for utilizing chestnut shell cultivating white mushroom, Pleurotus geesteranus, glossy ganoderma by " gordian technique of Chinese chestnut bud husk culturing edible fungus " that the people such as Qin Baoshan deliver.The method analyzes from the aspects such as upgrowth situation, fruiting performance and the biological transformation ratio of mycelia and straw, cotton seed hulls, and the result shows and utilizes chestnut shell cultivating white mushroom, Pleurotus geesteranus, glossy ganoderma can obtain beneficial effect.But the method adopts cooked material fermentation technique cultivating white mushroom, Pleurotus geesteranus, glossy ganoderma, uses high pressure steam equipment to medium sterilization in the cultivation process, causes its production cost high, and biological transformation ratio is low.
Summary of the invention
Technical problem to be solved by this invention is for above-mentioned the deficiencies in the prior art, a kind of substratum and cultivating method thereof of cultivating drumstick mushroom are provided, and this substratum is take chestnut shell as major ingredient, the green non-pollution cultivating drumstick mushroom, planting technique is simple, low production cost; The Coprinus comatus output of cultivating is high, quality better, biological transformation ratio are high.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of substratum of cultivating drumstick mushroom is made by the raw material of following mass parts: 30 parts~80 parts of chestnut shells, 10 parts~35 parts of crop material or corn cobs, just 9 parts~25 parts of poultry argol, 0.5 part~4 parts of calcium superphosphate, 0.2 part~3 parts of unslaked limes, 0.3 part~3 parts of enzymatic microorganism.
Further, made by the raw material of following mass parts: 46 parts~71 parts of chestnut shells, 15 parts~28 parts of crop material or corn cobs, just 12 parts~20 parts of poultry argol, 1 part~3 parts of calcium superphosphate, 0.5 part~1.5 parts of unslaked limes, 0.5 part~1.5 parts of enzymatic microorganism.
Further, made by the raw material of following mass parts: 61 parts of chestnut shells, just 15 parts of 20 parts of crop material or corn cobs, poultry argol, 2 parts of calcium superphosphate, 1 part of unslaked lime, 1 part of enzymatic microorganism.
Described crop material or corn cob, chestnut shell, poultry argol biodiversity content just all is no more than 15%; The substratum biodiversity content of cultivating drumstick mushroom is 60%~70%.
A kind of method of utilizing described substratum cultivating drumstick mushroom may further comprise the steps:
1) with chestnut shell, crop material or corn cob, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains base-material; Described chestnut shell, crop material or corn cob, poultry argol granularity just all≤3cm;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, kept 48h, turning is rebuild; Tear heap open according to " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " a plurality of all after dates of circulation, the biodiversity content of adjusting base-material is that 60%~70%, pH value is 7~8;
3) until step 2) in after the base-material temperature≤30 ℃ after the fermentation, with in its bacterium bag of packing into and connect strain of coprinus comatus, inoculum size is base-material dry weight 10%~15%, then is prepared into the bacterium rod;
4) the bacterium rod in the step 3) being placed temperature is that 20~25 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, space 3~7cm establishes in the mushroom bed, adds a cover the earthing that a layer thickness is 5~7cm after covering in the space between full bacterium rod;
6) management of spraying water keeps air and earthing moistening;
7) after sporophore is grown to six to eight minutes maturations, adopt mushroom.
The cross section of heap was the trapezoidal of the wide 1~1.2m in bottom, the wide 0.5~0.8m in top, high 0.8~1.2m when base-material step 2) was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 3~5cm, and the distance between breather hole is 30~50cm.
Bacterial classification layer and binder layer are alternately packed into during the pack of base-material described in the step 3), inoculate altogether 3~5 layers of bacterial classification, and it is ventilative to prick the hole in the bacterial classification layer position of bacterium bag.
The specification of the bag of bacterium described in the step 3) is: wide 20~26cm * length 40~60cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is planted along the bag wall.
The biodiversity content of earthing described in the step 5) is that 20%~30%, pH value is 7.5~8.0, manages through the deinsectization harm.
The management of water spray described in the step 6) is:
(1) the young flower bud phase: be under 16~26 ℃ the condition, to spray water in environment in envrionment temperature, the maintenance atmospheric moisture is 85RH%~90RH%, simultaneously to earthing spray form water, keeps earthing moistening, pinches flat loose for spending take soil block;
(2) the young mushroom phase: be 15~25 ℃ in envrionment temperature, illuminance is under the condition of 300~800lux, sprays water in environment, and the maintenance atmospheric moisture is 85RH%~90RH%, sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
(3) become the mushroom phase: be 15~30 ℃ in envrionment temperature, under the condition of illuminance≤1000lux, spray water in environment, maintenances atmospheric moisture is 85RH%~95RH%, sprays spray water xi to earthing and mushroom body simultaneously, and maintenance earthing and mushroom body are moistening.
The substratum of cultivating drumstick mushroom of the present invention, described calcium superphosphate, unslaked lime, enzymatic microorganism are the common commercially available agricultural supplie in this area.Described crop material is agricultural crop straw, and such as one or more the arbitrary proportion in Wheat Straw, soybean stalks, maize straw, the broomcorn straw etc., the concrete kind of selecting can be suited measures to local conditions.
Compared with prior art, the present invention has following useful technique effect:
(1) the present invention is take chestnut shell as the substratum major ingredient, adopt raw material fermentation technique cultivating drumstick mushroom, be the nutritive substance that easily absorbs of shaggy mane mycelium such as amino acid, polysaccharide, VITAMIN, active mineral matter etc. by microorganism with the organic substance decomposing of the complexity such as chestnut shell, crop material or corn cob, and make culture base-material become soft ventilative, water-absorbent and heat retaining property are good, in addition, tunning can the establishment miscellaneous bacteria growth, make shaggy mane mycelium obtain health, vigorous, sturdy growth, the biological transformation ratio of Coprinus comatus is up to 150%~200%.
(2) the present invention compares with traditional grog planting technique, and planting technique is simple, is easy to promote, and need not to use sterilizing device in the cultivation process, can reduce production costs 30%~40%;
(3) the present invention does not contain the harmful toxic matters such as agricultural chemical insecticide, sterilant, and the cultivation process satisfies the green non-pollution production requirement fully, really accomplishes non-polluted cultivation;
(4) adopt the contained nutritive ingredient of Coprinus comatus of the present invention's cultivation abundant, especially the content of the mineral elements such as lentinan, bioactive enzyme, unsaturated fatty acids and calcium, phosphorus, zinc, potassium is higher by 10%~30% than common cultivation method.
Description of drawings
Fig. 1 is the process flow sheet of cultivating drumstick mushroom.
Embodiment
The present invention is described in detail below in conjunction with accompanying drawing and specific embodiment, and the explanation of the invention is not limited.
The invention provides a kind of substratum of cultivating drumstick mushroom, by chestnut shell, crop material or corn cob, poultry argol just, calcium superphosphate, unslaked lime, enzymatic microorganism and water forms.In addition, the present invention also provides the method for utilizing this substratum cultivating drumstick mushroom, may further comprise the steps: one, that each raw material stirring is even, wherein tremble shell, crop material or corn cob to pulverize, and make base-material; Two, the base-material fermentation of banking up; Three, the bacterium rod is made in the pack of the base-material after will fermenting inoculation; Four, the bacterium rod places the bacteria chamber to cultivate; Five, the bacterium rod takes off bag, stands on earthing in the mushroom bed; Six, the management of spraying water; Seven, adopt mushroom.
Embodiment 1
The substratum of the cultivating drumstick mushroom of the present embodiment is made by the raw material of following mass parts: 80 parts of chestnut shells, and just 9 parts of 10 parts of corn cobs, poultry argol, 0.5 part of calcium superphosphate, 0.2 part of unslaked lime, 0.3 part of enzymatic microorganism, water is an amount of.
Referring to Fig. 1, the method for utilizing above-mentioned substratum cultivating drumstick mushroom of the present embodiment is as follows:
1) with chestnut shell, corn cob, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains biodiversity content and be 60% base-material;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, keep 48h, turning is rebuild, " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " three all after dates of circulation by this and tear heap open, the biodiversity content that detects and adjust base-material is that 60%, pH value is 7; The cross section of heap was the trapezoidal of the wide 1m in bottom, the wide 0.5m in top, high 0.8m when described base-material was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 3cm, and the distance between breather hole is 30cm;
3) with step 2) in base-material after the fermentation pack into and connect bacterial classification in the bacterium bag, bacterial classification layer and binder layer are alternately packed into during pack, inoculate altogether 3 layers of bacterial classification, and prick the hole in bacterial classification layer position ventilative, making inoculum size is the bacterium rod of culture material dry weight 10%; The specification of described bacterium bag is: wide 20cm * long 40cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is along one week of bag wall kind;
4) the postvaccinal bacterium rod of step 3) being placed temperature is that 20 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, interval 3cm establishes in the mushroom bed, adds a cover the earthing that thickness is 5cm after covering in full gap; The biodiversity content of described earthing is that 20%, pH value is 7.5, without insect pest;
6) in the young flower bud phase of mycelial growth, young mushroom phase with become the management of spraying water respectively of mushroom phase, concrete grammar is: (1) young flower bud phase: be 16 ℃ in envrionment temperature, atmospheric moisture is under the condition of 85RH%, in environment, spray water, simultaneously to the light spray form water of earthing, keep earthing moistening, pinch flat not loose as degree take soil block; (2) the young mushroom phase: be 15 ℃ in envrionment temperature, atmospheric moisture is 85RH%, and illuminance is under the condition of 300lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening; (3) become the mushroom phase: be 15 ℃ in envrionment temperature, atmospheric moisture is 85RH%, and illuminance is under the condition of 600lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
7) after the sporophore growth that becomes the mushroom phase described in the step 6) is six to eight minutes maturations, adopts mushroom, and in time manage the mushroom bed, adjust biodiversity content and the pH value of earthing, go out altogether 5 damp mushrooms.
Utilize the Coprinus comatus of substratum cultivation of the present embodiment healthy, vigorous, sturdy, biological transformation ratio is 150%.
Embodiment 2
The substratum of the cultivating drumstick mushroom of the present embodiment is made by the raw material of following mass parts: 71 parts of chestnut shells, and just 12 parts of 15 parts of maize straws, poultry argol, 1 part of calcium superphosphate, 0.5 part of unslaked lime, 0.5 part of enzymatic microorganism, water is an amount of.
The method of utilizing above-mentioned substratum cultivating drumstick mushroom of the present embodiment is as follows:
1) with chestnut shell, maize straw, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains biodiversity content and be 63% base-material;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, keep 48h, turning is rebuild, " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " three all after dates of circulation by this and tear heap open, the biodiversity content that detects and adjust base-material is that 63%, pH value is 7.3; The cross section of heap was the trapezoidal of the wide 1.2m in bottom, the wide 0.8m in top, high 0.9m when described base-material was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 3cm, and the distance between breather hole is 35cm;
3) with step 2) in base-material after the fermentation pack into and connect bacterial classification in the bacterium bag, bacterial classification layer and binder layer are alternately packed into during pack, inoculate altogether 3 layers of bacterial classification, and prick the hole in bacterial classification layer position ventilative, making inoculum size is the bacterium rod of culture material dry weight 11%; The specification of described bacterium bag is: wide 21cm * long 45cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is along one week of bag wall kind;
4) postvaccinal bacterium rod in the step 3) being placed temperature is that 21 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, interval 4cm establishes in the mushroom bed, adds a cover the earthing that thickness is 5cm after covering in full gap; The biodiversity content of described earthing is that 22%, pH value is 7.7, without insect pest;
6) in the young flower bud phase of mycelial growth, young mushroom phase with become the management of spraying water respectively of mushroom phase, concrete grammar is: (1) young flower bud phase: be 18 ℃ in envrionment temperature, atmospheric moisture is under the condition of 87RH%, in environment, spray water, simultaneously to the light spray form water of earthing, keep earthing moistening, pinch flat not loose as degree take soil block; (2) the young mushroom phase: be 18 ℃ in envrionment temperature, atmospheric moisture is 87RH%, and illuminance is under the condition of 400lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening; (3) become the mushroom phase: be 17 ℃ in envrionment temperature, atmospheric moisture is 87RH%, and illuminance is under the condition of 1000lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
7) after the sporophore growth that becomes the mushroom phase described in the step 6) is six to eight minutes maturations, adopts mushroom, and in time manage the mushroom bed, adjust biodiversity content and the pH value of earthing, go out altogether 4 damp mushrooms.
Utilize the Coprinus comatus of substratum cultivation of the present embodiment healthy, vigorous, sturdy, biological transformation ratio is 160%.
Embodiment 3
The substratum of the cultivating drumstick mushroom of the present embodiment is made by the raw material of following mass parts: 61 parts of chestnut shells, and wheat, 20 parts of maize straws (mass ratio 1:1), just 15 parts of poultry argol, 2 parts of calcium superphosphate, 1 part of unslaked lime, 1 part of enzymatic microorganism, water is an amount of.
The method of utilizing above-mentioned substratum cultivating drumstick mushroom of the present embodiment is as follows:
1) with chestnut shell, wheat stalk, maize straw, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains biodiversity content and be 65% base-material;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, keep 48h, turning is rebuild, " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " three all after dates of circulation by this and tear heap open, the biodiversity content that detects and adjust base-material is that 65%, pH value is 7.5; The cross section of heap was the trapezoidal of the wide 1.5m in bottom, the wide 0.7m in top, high 1.0m when described base-material was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 4cm, and the distance between breather hole is 40cm;
3) with step 2) in base-material after the fermentation pack into and connect bacterial classification in the bacterium bag, bacterial classification layer and binder layer are alternately packed into during pack, inoculate altogether 4 layers of bacterial classification, and prick the hole in bacterial classification layer position ventilative, making inoculum size is the bacterium rod of culture material dry weight 12%; The specification of described bacterium bag is: wide 23cm * long 50cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is along one week of bag wall kind;
4) postvaccinal bacterium rod in the step 3) being placed temperature is that 22 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, interval 5cm establishes in the mushroom bed, adds a cover the earthing that thickness is 5cm after covering in full gap; The biodiversity content of described earthing is that 25%, pH value is 7.8, without insect pest;
6) in the young flower bud phase of mycelial growth, young mushroom phase with become the management of spraying water respectively of mushroom phase, concrete grammar is: (1) young flower bud phase: be 20 ℃ in envrionment temperature, atmospheric moisture is under the condition of 88RH%, in environment, spray water, simultaneously to the light spray form water of earthing, keep earthing moistening, pinch flat not loose as degree take soil block; (2) the young mushroom phase: be 20 ℃ in envrionment temperature, atmospheric moisture is 88RH%, and illuminance is under the condition of 600lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening; (3) become the mushroom phase: be 20 ℃ in envrionment temperature, atmospheric moisture is 90RH%, and illuminance is under the condition of 800lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
7) after the sporophore growth that becomes the mushroom phase described in the step 6) is six to eight minutes maturations, adopts mushroom, and in time manage the mushroom bed, adjust biodiversity content and the pH value of earthing, go out altogether 4 damp mushrooms.
Utilize the Coprinus comatus of substratum cultivation of the present embodiment healthy, vigorous, sturdy, biological transformation ratio is 170%.
Embodiment 4
The substratum of the cultivating drumstick mushroom of the present embodiment is made by the raw material of following mass parts: 46 parts of chestnut shells, and just 20 parts of 28 parts of crop material or corn cobs, poultry argol, 3 parts of calcium superphosphate, 1.5 parts of unslaked limes, 0.5 part of enzymatic microorganism, water is an amount of.
The method of utilizing above-mentioned substratum cultivating drumstick mushroom of the present embodiment is as follows:
1) with chestnut shell, crop material or corn cob, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains biodiversity content and be 68% base-material;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, keep 48h, turning is rebuild, " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " three all after dates of circulation by this and tear heap open, the biodiversity content that detects and adjust base-material is that 68%, pH value is 7.8; The cross section of heap was the trapezoidal of the wide 1.8m in bottom, the wide 0.8m in top, high 1.1m when described base-material was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 4cm, and the distance between breather hole is 45cm;
3) with step 2) in base-material after the fermentation pack into and connect bacterial classification in the bacterium bag, bacterial classification layer and binder layer are alternately packed into during pack, inoculate altogether 4 layers of bacterial classification, and prick the hole in bacterial classification layer position ventilative, making inoculum size is the bacterium rod of culture material dry weight 14%; The specification of described bacterium bag is: wide 25cm * long 55cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is along one week of bag wall kind;
4) postvaccinal bacterium rod in the step 3) being placed temperature is that 23 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, interval 6cm establishes in the mushroom bed, adds a cover the earthing that thickness is 6cm after covering in full gap; The biodiversity content of described earthing is that 28%, pH value is 7.8, without insect pest;
6) in the young flower bud phase of mycelial growth, young mushroom phase with become the management of spraying water respectively of mushroom phase, concrete grammar is: (1) young flower bud phase: be 24 ℃ in envrionment temperature, atmospheric moisture is under the condition of 89RH%, in environment, spray water, simultaneously to the light spray form water of earthing, keep earthing moistening, pinch flat not loose as degree take soil block; (2) the young mushroom phase: be 22 ℃ in envrionment temperature, atmospheric moisture is 88RH%, and illuminance is under the condition of 700lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening; (3) become the mushroom phase: be 26 ℃ in envrionment temperature, atmospheric moisture is 90RH%, and illuminance is under the condition of 500lux, sprays water in environment, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
7) after the sporophore growth that becomes the mushroom phase described in the step 6) is six to eight minutes maturations, adopts mushroom, and in time manage the mushroom bed, adjust biodiversity content and the pH value of earthing, go out altogether 4 damp mushrooms.
Utilize the Coprinus comatus of substratum cultivation of the present embodiment healthy, vigorous, sturdy, biological transformation ratio is 180%.
Embodiment 5
The substratum of the cultivating drumstick mushroom of the present embodiment is made by the raw material of following mass parts: 30 parts of chestnut shells, and just 25 parts of 35 parts of crop material or corn cobs, poultry argol, 4 parts of calcium superphosphate, 3 parts of unslaked limes, 3 parts of enzymatic microorganism, water is an amount of.
The method of utilizing above-mentioned substratum cultivating drumstick mushroom of the present embodiment is as follows:
1) with chestnut shell, crop material or corn cob, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains biodiversity content and be 70% base-material;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, keep 48h, turning is rebuild, " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " three all after dates of circulation by this and tear heap open, the biodiversity content that detects and adjust base-material is that 70%, pH value is 8; The cross section of heap was the trapezoidal of the wide 2m in bottom, the wide 1m in top, high 1.2m when described base-material was built the heap fermentation, and the top of heap vertically is equipped with the breather hole that a plurality of diameters are 5cm, and the distance between breather hole is 50cm;
3) with step 2) in base-material after the fermentation pack into and connect bacterial classification in the bacterium bag, bacterial classification layer and binder layer are alternately packed into during pack, inoculate altogether 5 layers of bacterial classification, and prick the hole in bacterial classification layer position ventilative, making inoculum size is the bacterium rod of culture material dry weight 15%; The specification of described bacterium bag is: wide 26cm * long 60cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is along one week of bag wall kind;
4) postvaccinal bacterium rod in the step 3) being placed temperature is that 25 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, interval 7cm establishes in the mushroom bed, adds a cover the earthing that thickness is 7cm after covering in full gap; The biodiversity content of described earthing is that 30%, pH value is 8.0, without insect pest;
6) in the young flower bud phase of mycelial growth, young mushroom phase with become the management of spraying water respectively of mushroom phase, concrete grammar is: (1) young flower bud phase: be under 26 ℃ the condition in envrionment temperature, in environment, spray water, the maintenance atmospheric moisture is 90RH%, simultaneously to the light spray form water of earthing, keep earthing moistening, pinch flat not loose as degree take soil block; (2) the young mushroom phase: be 25 ℃ in envrionment temperature, illuminance is under the condition of 800lux, sprays water in environment, and the maintenance atmospheric moisture is 90RH%, suitably sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening; (3) become the mushroom phase: be 30 ℃ in envrionment temperature, illuminance is under the condition of 400lux, sprays water in environment, and maintenances atmospheric moisture is 95RH%, suitably sprays spray water xi to earthing and mushroom body simultaneously, and maintenance earthing and mushroom body are moistening;
7) after the sporophore growth that becomes the mushroom phase described in the step 6) is six to eight minutes maturations, adopts mushroom, and in time manage the mushroom bed, adjust biodiversity content and the pH value of earthing, go out altogether 4 damp mushrooms.
Utilize the Coprinus comatus of substratum cultivation of the present embodiment healthy, vigorous, sturdy, biological transformation ratio is 200%.
The above only is preferred embodiment of the present invention, is not that the present invention is imposed any restrictions.Every any simple modification, change and equivalence of above embodiment being done according to the invention technical spirit changes, and all still belongs in the protection domain of technical solution of the present invention.
Claims (10)
1. the substratum of a cultivating drumstick mushroom is characterized in that, is made by the raw material of following mass parts: 30 parts~80 parts of chestnut shells, 10 parts~35 parts of crop material or corn cobs, just 9 parts~25 parts of poultry argol, 0.5 part~4 parts of calcium superphosphate, 0.2 part~3 parts of unslaked limes, 0.3 part~3 parts of enzymatic microorganism.
2. the substratum of a kind of cultivating drumstick mushroom according to claim 1, it is characterized in that, raw material by following mass parts is made: 46 parts~71 parts of chestnut shells, 15 parts~28 parts of crop material or corn cobs, just 12 parts~20 parts of poultry argol, 1 part~3 parts of calcium superphosphate, 0.5 part~1.5 parts of unslaked limes, 0.5 part~1.5 parts of enzymatic microorganism.
3. the substratum of a kind of cultivating drumstick mushroom according to claim 2 is characterized in that, is made by the raw material of following mass parts: 61 parts of chestnut shells, just 15 parts of 20 parts of crop material or corn cobs, poultry argol, 2 parts of calcium superphosphate, 1 part of unslaked lime, 1 part of enzymatic microorganism.
4. according to claim 1, the substratum of 2 or 3 described a kind of cultivating drumstick mushrooms, it is characterized in that, described crop material or corn cob, chestnut shell, poultry argol biodiversity content just all is no more than 15%; The substratum biodiversity content of cultivating drumstick mushroom is 60%~70%.
5. a method of utilizing claim 1,2 or 3 described substratum cultivating drumstick mushrooms is characterized in that, may further comprise the steps:
1) with chestnut shell, crop material or corn cob, poultry argol just, calcium superphosphate, unslaked lime and enzymatic microorganism add water by mass parts and stir, and obtains base-material; Described chestnut shell, crop material or corn cob, poultry argol granularity just all≤3cm;
2) base-material described in the step 1) is built the heap fermentation, after the temperature of base-material rises to 60 ℃, kept 48h, turning is rebuild; Tear heap open according to " build heap-material temperature and rise to 60 ℃-maintenance 48h-turning reconstruction " a plurality of all after dates of circulation, the biodiversity content of adjusting base-material is that 60%~70%, pH value is 7~8;
3) until step 2) in after the base-material temperature≤30 ℃ after the fermentation, with in its bacterium bag of packing into and connect strain of coprinus comatus, inoculum size is base-material dry weight 10%~15%, then is prepared into the bacterium rod;
4) the bacterium rod in the step 3) being placed temperature is that 20~25 ℃ bacteria chamber is cultured to the mycelia purseful, during in time choose except miscellaneous bacteria;
5) the bacterium rod that covers with mycelia in the step 4) is taken off bag, space 3~7cm establishes in the mushroom bed, adds a cover the earthing that a layer thickness is 5~7cm after covering in the space between full bacterium rod;
6) management of spraying water keeps air and earthing moistening;
7) after sporophore is grown to six to eight minutes maturations, adopt mushroom.
6. the method for cultivating drumstick mushroom according to claim 5, it is characterized in that, the cross section of heap was the trapezoidal of the wide 1~1.2m in bottom, the wide 0.5~0.8m in top, high 0.8~1.2m when base-material step 2) was built the heap fermentation, the top of heap vertically is equipped with the breather hole that a plurality of diameters are 3~5cm, and the distance between breather hole is 30~50cm.
7. the method for cultivating drumstick mushroom according to claim 5 is characterized in that, bacterial classification layer and binder layer are alternately packed into during the pack of base-material described in the step 3), inoculate altogether 3~5 layers of bacterial classification, and it is ventilative to prick the hole in the bacterial classification layer position of bacterium bag.
8. the method for cultivating drumstick mushroom according to claim 5, it is characterized in that, the specification of the bag of bacterium described in the step 3) is: wide 20~26cm * length 40~60cm, the bacterial classification of bacterium bag top and bottom concentrate and plant in middle position, and the bacterial classification at bacterium bag middle part is planted along the bag wall.
9. the method for cultivating drumstick mushroom according to claim 5 is characterized in that, the biodiversity content of earthing described in the step 5) is that 20%~30%, pH value is 7.5~8.0, manages through the deinsectization harm.
10. the method for cultivating drumstick mushroom according to claim 5 is characterized in that, the management of water spray described in the step 6) is:
(1) the young flower bud phase: be under 16~26 ℃ the condition, to spray water in environment in envrionment temperature, the maintenance atmospheric moisture is 85RH%~90RH%, simultaneously to earthing spray form water, keeps earthing moistening, pinches flat loose for spending take soil block;
(2) the young mushroom phase: be 15~25 ℃ in envrionment temperature, illuminance is under the condition of 300~800lux, sprays water in environment, and the maintenance atmospheric moisture is 85RH%~90RH%, sprays spray water xi to earthing and mushroom body simultaneously, keeps earthing and mushroom body moistening;
(3) become the mushroom phase: be 15~30 ℃ in envrionment temperature, under the condition of illuminance≤1000lux, spray water in environment, maintenances atmospheric moisture is 85RH%~95RH%, sprays spray water xi to earthing and mushroom body simultaneously, and maintenance earthing and mushroom body are moistening.
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