CN106613334A - Nutrient-rich pleurotus eryngii cultivation method - Google Patents

Nutrient-rich pleurotus eryngii cultivation method Download PDF

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Publication number
CN106613334A
CN106613334A CN201611044835.2A CN201611044835A CN106613334A CN 106613334 A CN106613334 A CN 106613334A CN 201611044835 A CN201611044835 A CN 201611044835A CN 106613334 A CN106613334 A CN 106613334A
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culture
deionized water
chinese chestnut
water
chestnut bud
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唐修志
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Yingshang County Duo Tang Lake Of Modern Agricultural Science And Technology Co Ltd
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Yingshang County Duo Tang Lake Of Modern Agricultural Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Abstract

The invention discloses a nutrient-rich pleurotus eryngii cultivation method. According to the provided nutrient-rich pleurotus eryngii cultivation method, various nutrients needed by the growth of pleurotus eryngii can be provided, the fertilizer efficiency is high, the mushroom yield is fast, neat, uniform and strong, the yield is high, the number of picking crops is big, the production cost is low, and the application prospect is good.

Description

A kind of nutritious method for planting almond abalone mushroom
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of nutritious method for planting almond abalone mushroom.
Background technology
With the progress of science and technology, mushroom industry fast development, the cultivation two of edible mushroom is thus right constantly increasing The demand of culturing raw material increases increasingly.In edible mushroom substituting stuff cultivation technology, traditional cultivation raw material mainly have cottonseed, bagasse, Weed tree sawdust, crop material etc..With people it is increasingly mature to the technology of these raw material comprehensive utilization, the purposes of these raw materials More extensive, such as wood chip is used to process synthetic plate, and bagasse is used for papermaking etc., this provides for improved Edible Fungi cost, nothing The pressure of Edible Fungi enterprise, therefore research and development novel edible fungus culturing raw material are increased in shape, product cost is reduced into, Increase economic efficiency, have great importance.China is the big country of Chinese chestnut plantation, in Chinese chestnut growing area, annual harvesting Chinese chestnut it Substantial amounts of leftover bits and pieces-Chinese chestnut bud husk can be all produced afterwards.Comprehensive utilizating research and exploitation of the current people to Chinese chestnut bud husk, mostly The aspects such as solid shape carbon production, tannin extract and nature strength are all concentrated on, because Chinese chestnut bud husk contains carbon, nitrogen and mineral matter etc. Composition, it is possible to provide the required basic nutrition of edible fungi growth development.
Qin Baoshan exists《The test of Chinese chestnut bud husk Xinbao mushroom culturing》With Chinese chestnut bud husk as main carbon source material in one text, with rice Grass, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits etc. for auxiliary carbon source material, with rice bran as nitrogen source, arrange 10 process carry out apricot Abalone mushroom experiment in cultivation, from the commodity property of mycelial growth situation, growth cycle, fructification properties and characteristicses, biological efficiency and mushroom Etc. aspect be analyzed, as a result show:It is feasible using Chinese chestnut bud husk and Chinese chestnut leaf Xinbao mushroom culturing, individually with Chinese chestnut Luxuriant shell is for carbon source material or adds the materials such as appropriate straw, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits in Chinese chestnut bud husk, Obtain preferable cultivation effect.But the tannin in Chinese chestnut bud husk is more, then when carrying out cultivating flat mushroom and elegant precious mushroom, training Foster yield is not high, and luxuriant shell size must be suitable, and not enough, easy breed bacteria is too big easily to stab for the permeability of too little culture medium Broken plastic foil, causes humiture not enough, affects the yield of edible mushroom, and test consumption above is less, and is only to make For the compost of pleurotus eryngii, so Chinese chestnut bud husk must be done into the culture demand that further processing meets multiple edible mushroom, allow Chinese chestnut bud husk is reasonably utilized, and prevents the wasting of resources, reduces production cost, improves the economic and social benefits.
The content of the invention
The object of the invention is exactly to make up the defect of prior art, there is provided a kind of nutritious planting almond abalone mushroom side Method.
The present invention is achieved by the following technical solutions:
A kind of nutritious method for planting almond abalone mushroom, including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30 Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity For one-level kind;
The Mother culture based formulas are:Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min, Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs Mix and add appropriate deionized water after dissolving, be sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 40min, is cooled to standby after room temperature;Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/ L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h, Separation of solid and liquid, it is standby after filter residue is dried, treat banana stalk is cut into into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively The 24h in 3% limewash, taking-up drains rear and Chinese chestnut bud husk extract, corncob, lactic acid, rice bran, gypsum and waste residue of Chinese herbs According to half sponge process process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, keeps at 50 DEG C 4 days, make the water content of compost between 60-65% after fermentation ends, pH value is 7-7.5;
The formula of the compost is according to mass percent proportioning:Chinese chestnut bud husk 55-70%, treat banana stalk 15-20%, rice bran 13- 17%th, corncob 14-16%, waste residue of Chinese herbs 1.3-3%, gypsum 1-1.5%, lactic acid 0.4-0.7%, appropriate deionized water;
B, sodium alginate is added in deionized water, heating stirring dissolves it, controls its mass concentration in 1.8-3%, treats that its is complete Water-absorbing resin ultrasonic agitation is added after CL uniformly, the addition of wherein sodium alginate is the 30% of water-absorbing resin weight, stood Bubble is removed, it is in 5% calcium chloride solution, by product then the dropper of mixed solution diameter 1.5cm to be added drop-wise to into mass concentration Deionized water is rinsed 3 times, and 8h is extracted at 80 DEG C with absolute ethyl alcohol after vacuum drying, is dried, then is taken out at 60 DEG C with acetone 8h is carried, it is standby after vacuum drying;
C, the material for preparing step a and step b are according to mass ratio 20:1 mixing and stirring, then using the poly- of appropriate gauge The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, tightens and be placed in after sack sterilizing in high-pressure sterilizing pot, and 126 DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training In nutriment, according still further to sterile working inoculation, inoculum concentration covers charge level and is advisable with bacterial classification;
Described nutrient solution prescription is:Calcium phosphate 4-5ppm, potassium nitrate 6-8ppm, magnesium sulfate 6-8ppm, ammonium phosphate 4-6ppm, sulphur Sour ferrous iron 7-9ppm, trace element 2-3ppm, brown coal wash heat liquid to 1000mL;
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18- 28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and after the long purseful of mycelia mushroom house is produced;
E, select water quality it is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 2-3% volcanic rock, the concave convex rod of 3-5% The deer natural pond soil mixing and stirring of soil and 5-8%, then adds 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% Carbendazim, be mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then will step Bacterium bag in rapid d carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation Condition.
It is an advantage of the invention that:Chinese chestnut bud husk is spherical, the close involucre being needled in Castanea mollissima Nut outside, due to edible mushroom Become adult come edible good protein by decomposing, converting the organic matter for going out of use, and Chinese chestnut bud husk contains carbon, nitrogen and ore deposit The compositions such as material, can be as the required basic nutrition of edible fungi growth development, so the present invention is by after Chinese chestnut bud husk and liquor Chinese chestnut bud husk slag as main charcoal source material, the juice of Chinese chestnut bud husk as zymotic fluid, then be aided with treat banana stalk, corncob, in The compost of medicine waste residue, gypsum and lactic acid as edible mushroom, it is ensured that demand of the edible mushroom to carbon source, nitrogen source and other nutrition, and And water-absorbing resin microballoon is prepared by chemical reaction, with good water suction, water conservation and sustained release performance, can by nutrient solution and When be supplied to pleurotus eryngii, drought-resistant and fertilizer, it is ensured that the supply and demand of its nutrition so as to send out that bacterium is fast, wire vent is more, reduces what later stage fertilizer supplied water Labour operates, time saving and energy saving, cost-effective, and will appear from the mushroom of former base and carry out vallum plantation, covers homemade Nutrition Soil, Pluck mushrooms for many batches of later stage and nutrition be provided so as to which mushroom growing up number is more, yield height, and for Chinese chestnut bud husk resource huge profit with looking for To new approach, the pollution to environment is reduced, the cultural method that the present invention is provided can be each for needed for pleurotus eryngii provides growth Nutrition is planted, fertilizer efficiency is high, fruiting is fast, neat, even, strong and yield is high, harvesting stubble number is more, and low production cost, application prospect is good.
Specific embodiment
A kind of nutritious method for planting almond abalone mushroom, including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, at 20 DEG C At a temperature of cultivate, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity One-level kind;
The Mother culture based formulas are:Potato mixed juice 200g/L, agar 12g/L, sucrose 20g/L, peptone 5g/L, work Property charcoal 0.3g/L, adds water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30min, filter Residue, merging filtrate is gone simultaneously to add dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, be sufficiently stirred for Appropriate deionized water is added after dissolving, is sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 40min, is cooled to standby after room temperature;Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160g/L, Chinese chestnut bud husk 40g/L, dusty yeast 5g/L, glucose 20g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h, Separation of solid and liquid, it is standby after filter residue is dried, treat banana stalk is cut into into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively The 24h in 3% limewash, taking-up drains rear and Chinese chestnut bud husk extract, corncob, lactic acid, rice bran, gypsum and waste residue of Chinese herbs According to half sponge process process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, keeps at 50 DEG C 4 days, make the water content of compost between 60% after fermentation ends, pH value is 7;
The formula of the compost is according to mass percent proportioning:Chinese chestnut bud husk 55%, treat banana stalk 15%, rice bran 13%, corncob 14%th, waste residue of Chinese herbs 1.3%, gypsum 1%, lactic acid 0.4%, appropriate deionized water;
B, sodium alginate is added in deionized water, heating stirring dissolves it, controls its mass concentration 1.8%, treats that its is complete Water-absorbing resin ultrasonic agitation is added after dissolving uniformly, the addition of wherein sodium alginate is the 30% of water-absorbing resin weight, and standing is removed Bubble is removed, then the dropper of mixed solution diameter 1.5cm mass concentration is added drop-wise in 5% calcium chloride solution, product to be used Deionized water rinsing 3 times, 8h is extracted after vacuum drying with absolute ethyl alcohol at 80 DEG C, is dried, then is extracted at 60 DEG C with acetone 8h, it is standby after vacuum drying;
C, the material for preparing step a and step b are according to mass ratio 20:1 mixing and stirring, then using the poly- of appropriate gauge The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, tightens and be placed in after sack sterilizing in high-pressure sterilizing pot, and 126 DEG C 2h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into compost In, according still further to sterile working inoculation, inoculum concentration covers charge level and is advisable with bacterial classification;
Described nutrient solution prescription is:Calcium phosphate 4ppm, potassium nitrate 6ppm, magnesium sulfate 6ppm, ammonium phosphate 4ppm, ferrous sulfate 7ppm, trace element 2ppm, brown coal wash heat liquid to 1000mL;
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is 18 DEG C, relative air humidity is 65%, and a culture bag was turned over every 7 days, and after the long purseful of mycelia mushroom house is produced;
E, select water quality it is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 2% volcanic rock, 3% attapulgite and 5% deer natural pond soil mixing and stirring, then adds 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% many bacterium Spirit, is mixed into Nutrition Soil, and overlay film boring cover 2 days, and the humidity for keeping Nutrition Soil is 45%, then by the bacterium bag in step d Vallum earthing is carried out, the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
The upgrowth situation of hypha of edible fungus is compared in order to embody Chinese chestnut bud husk, according to following formula different cultures are prepared Material is cultivated pleurotus eryngii, and to the indices of growth course observation comparison is carried out
A Chinese chestnut bud husks 55%, treat banana stalk 15%, rice bran 13%, corncob 14%, waste residue of Chinese herbs 1.3%, gypsum 1%, lactic acid 0.4%
B glucose 55%, treat banana stalk 15%, rice bran 13%, corncob 14%, waste residue of Chinese herbs 1.3%, gypsum 1%, lactic acid 0.4%
C Chinese chestnut bud husks 70%, rice bran 13%, corncob 14%, waste residue of Chinese herbs 1.3%, gypsum 1%, lactic acid 0.4%
D glucose 70%, rice bran 13%, corncob 14%, waste residue of Chinese herbs 1.3%, gypsum 1%, lactic acid 0.4%
The mycelial growth situation of control
Long speed(mm/d)Mycelial density mycelia thickness mycelia color and luster
A 4.31 +++ ++ it is relatively thick dense white
B 3.94 ++++thin dense white
C 4.09 ++++thick dense white
D 3.78 +++ it is relatively thin white
"+" number represents mycelial density, and "+" represents that density is bigger
Growth cycle compares
There is former base(d)Fructification grows up to(d)Growth cycle(d)
A 7 6.9 81
B 8 8.4 86
C 7 7.4 84
D 9 8.8 91
The properties and characteristicses of each formula fructification
The mono- mushroom weight/g unit weights/g/mL of stem diameter/cm stems length/cm
A 3.8 11.25 53.99 0.75
B 3.4 10.86 42.23 0.83
C 3.7 11.05 46.36 0.78
D 3.1 10.26 38.58 0.88

Claims (1)

1. a kind of nutritious method for planting almond abalone mushroom, it is characterised in that including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30 Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity For one-level kind;
The Mother culture based formulas are:Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min, Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs Mix and add appropriate deionized water after dissolving, be sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings 40min, is cooled to standby after room temperature;Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/ L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h, Separation of solid and liquid, it is standby after filter residue is dried, treat banana stalk is cut into into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively The 24h in 3% limewash, taking-up drains rear and Chinese chestnut bud husk extract, corncob, lactic acid, gypsum and waste residue of Chinese herbs according to two Secondary fermentation method is processed, primary fermentation 18 days, altogether turning 4 times, and then overlay film fermentation, keeps 10h at 60 DEG C, is kept for 4 days at 50 DEG C, is sent out Ferment makes the water content of compost between 60-65% after terminating, pH value is 7-7.5;
The formula of the compost is according to mass percent proportioning:Chinese chestnut bud husk 55-70%, treat banana stalk 30-40%, corncob 4- 6%th, waste residue of Chinese herbs 1.7-3%, gypsum 1-1.5%, lactic acid 0.4-0.7%, appropriate deionized water;
B, sodium alginate is added in deionized water, heating stirring dissolves it, controls its mass concentration in 1.8-3%, treats that its is complete Water-absorbing resin ultrasonic agitation is added after CL uniformly, the addition of wherein sodium alginate is the 30% of water-absorbing resin weight, stood Bubble is removed, it is in 5% calcium chloride solution, by product then the dropper of mixed solution diameter 1.5cm to be added drop-wise to into mass concentration Deionized water is rinsed 3 times, and 8h is extracted at 80 DEG C with absolute ethyl alcohol after vacuum drying, is dried, then is taken out at 60 DEG C with acetone 8h is carried, it is standby after vacuum drying;
C, the material for preparing step a and step b are according to mass ratio 20:1 mixing and stirring, then using the poly- of appropriate gauge The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, tightens and be placed in after sack sterilizing in high-pressure sterilizing pot, and 126 DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training In nutriment, according still further to sterile working inoculation, inoculum concentration covers charge level and is advisable with bacterial classification;
Described nutrient solution prescription is:Calcium phosphate 4-5ppm, potassium nitrate 6-8ppm, magnesium sulfate 6-8ppm, ammonium phosphate 4-6ppm, sulphur Sour ferrous iron 7-9ppm, trace element 2-3ppm, brown coal wash heat liquid to 1000mL;
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18- 28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and after the long purseful of mycelia mushroom house is produced;
E, select water quality it is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 2-3% volcanic rock, the concave convex rod of 3-5% The deer natural pond soil mixing and stirring of soil and 5-8%, then adds 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% Carbendazim, be mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then will step Bacterium bag in rapid d carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation Condition.
CN201611044835.2A 2016-11-24 2016-11-24 Nutrient-rich pleurotus eryngii cultivation method Pending CN106613334A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107151185A (en) * 2017-07-10 2017-09-12 天津中祥农业科技发展有限公司 A kind of pleurotus eryngii culture medium and preparation method thereof
CN107602261A (en) * 2017-10-27 2018-01-19 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of pleurotus eryngii and preparation method thereof
CN108934749A (en) * 2018-06-28 2018-12-07 靖西市秀美边城农业科技有限公司 A kind of cultural method improving Pleurotus eryngii quality
RU2692636C1 (en) * 2017-12-29 2019-06-25 Федеральное государственное бюджетное образовательное учреждение высшего образования "Государственный аграрный университет Северного Зауралья" (ФГБОУ ВО ГАУ Северного Зауралья) Method of preparing a culture medium for growing a pure mycelium pleurotus ostreatus culture
CN110036826A (en) * 2019-03-26 2019-07-23 海南达川食品有限公司 It is a kind of using mango core as the cultivation of glossy ganoderma matrix and preparation method of major ingredient

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1362011A (en) * 2002-02-01 2002-08-07 山东省农业科学院土壤肥料研究所 Xinbao mushroom culturing method utilizing corn stalk
CN1563347A (en) * 2004-04-04 2005-01-12 浙江大学 Method for solidifying liquid fungus germ of edible fungus
CN101444170A (en) * 2008-12-16 2009-06-03 许忠 Strain separation method of apricot ormer mushroom and cultivating method thereof
CN101863704A (en) * 2010-06-21 2010-10-20 浙江海洋学院 Pleurotus eryngii culture medium and culture method thereof
CN102924156A (en) * 2012-11-22 2013-02-13 柞水县海林菌业有限责任公司 Culture medium and cultural method for cultivating coprinus comatus
CN104946540A (en) * 2015-06-11 2015-09-30 天津中天精科科技有限公司 Pleurotus eryngii Quel. liquid strain capable of preventing mixed bacterium infection during cultivation and fermentation method of pleurotus eryngii Quel liquid strain
CN105009937A (en) * 2015-06-29 2015-11-04 吴华球 Cultivation method for pleurotus geesteranus
CN105532257A (en) * 2015-12-07 2016-05-04 武文礼 Pleurotusostreatus and pleurotus eryngii high-yield cultivation method
CN105732184A (en) * 2016-01-14 2016-07-06 河池学院 Culture medium and method for cultivating lucid ganoderma by utilizing chestnut bracteal shells

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1362011A (en) * 2002-02-01 2002-08-07 山东省农业科学院土壤肥料研究所 Xinbao mushroom culturing method utilizing corn stalk
CN1563347A (en) * 2004-04-04 2005-01-12 浙江大学 Method for solidifying liquid fungus germ of edible fungus
CN101444170A (en) * 2008-12-16 2009-06-03 许忠 Strain separation method of apricot ormer mushroom and cultivating method thereof
CN101863704A (en) * 2010-06-21 2010-10-20 浙江海洋学院 Pleurotus eryngii culture medium and culture method thereof
CN102924156A (en) * 2012-11-22 2013-02-13 柞水县海林菌业有限责任公司 Culture medium and cultural method for cultivating coprinus comatus
CN104946540A (en) * 2015-06-11 2015-09-30 天津中天精科科技有限公司 Pleurotus eryngii Quel. liquid strain capable of preventing mixed bacterium infection during cultivation and fermentation method of pleurotus eryngii Quel liquid strain
CN105009937A (en) * 2015-06-29 2015-11-04 吴华球 Cultivation method for pleurotus geesteranus
CN105532257A (en) * 2015-12-07 2016-05-04 武文礼 Pleurotusostreatus and pleurotus eryngii high-yield cultivation method
CN105732184A (en) * 2016-01-14 2016-07-06 河池学院 Culture medium and method for cultivating lucid ganoderma by utilizing chestnut bracteal shells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
覃宝山等: "《板栗苞壳栽培杏鲍菇的试验》", 《河池学院学报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107151185A (en) * 2017-07-10 2017-09-12 天津中祥农业科技发展有限公司 A kind of pleurotus eryngii culture medium and preparation method thereof
CN107602261A (en) * 2017-10-27 2018-01-19 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of pleurotus eryngii and preparation method thereof
RU2692636C1 (en) * 2017-12-29 2019-06-25 Федеральное государственное бюджетное образовательное учреждение высшего образования "Государственный аграрный университет Северного Зауралья" (ФГБОУ ВО ГАУ Северного Зауралья) Method of preparing a culture medium for growing a pure mycelium pleurotus ostreatus culture
CN108934749A (en) * 2018-06-28 2018-12-07 靖西市秀美边城农业科技有限公司 A kind of cultural method improving Pleurotus eryngii quality
CN110036826A (en) * 2019-03-26 2019-07-23 海南达川食品有限公司 It is a kind of using mango core as the cultivation of glossy ganoderma matrix and preparation method of major ingredient

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