CN106588279A - Cultivation method of selenium-rich edible mushrooms - Google Patents
Cultivation method of selenium-rich edible mushrooms Download PDFInfo
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- CN106588279A CN106588279A CN201611044024.2A CN201611044024A CN106588279A CN 106588279 A CN106588279 A CN 106588279A CN 201611044024 A CN201611044024 A CN 201611044024A CN 106588279 A CN106588279 A CN 106588279A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a cultivation method of selenium-rich edible mushrooms capable of preventing diseases efficiently. The cultivation method of the selenium-rich edible mushrooms is simple and easy to perform, sources of raw materials are wide, the production cost and the pollution to the environment are reduced, the time and labor are saved, mass production can be realized, produced edible mushroom fruiting bodies are big, white, tender and fleshy, the yield is high, and considerable economic benefits and social benefits can be brought.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of cultural method of selenium-enriched edible mushroom.
Background technology
With the progress of science and technology, mushroom industry fast development, the cultivation two of edible mushroom is thus right constantly increasing
The demand of culturing raw material increases increasingly.In edible mushroom substituting stuff cultivation technology, traditional cultivation raw material mainly have cottonseed, bagasse,
Weed tree sawdust, crop material etc..With people it is increasingly mature to the technology of these raw material comprehensive utilization, the purposes of these raw materials
More extensive, such as wood chip is used to process synthetic plate, and bagasse is used for papermaking etc., this provides for improved Edible Fungi cost, nothing
The pressure of Edible Fungi enterprise, therefore research and development novel edible fungus culturing raw material are increased in shape, product cost is reduced into,
Increase economic efficiency, have great importance.China is the big country of Chinese chestnut plantation, in Chinese chestnut growing area, annual harvesting Chinese chestnut it
Substantial amounts of leftover bits and pieces-Chinese chestnut bud husk can be all produced afterwards.Comprehensive utilizating research and exploitation of the current people to Chinese chestnut bud husk, mostly
The aspects such as solid shape carbon production, tannin extract and nature strength are all concentrated on, because Chinese chestnut bud husk contains carbon, nitrogen and mineral matter etc.
Composition, it is possible to provide the required basic nutrition of edible fungi growth development.
Qin Baoshan exists《The test of Chinese chestnut bud husk Xinbao mushroom culturing》With Chinese chestnut bud husk as main carbon source material in one text, with rice
Grass, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits etc. for auxiliary carbon source material, with rice bran as nitrogen source, arrange 10 process carry out apricot
Abalone mushroom experiment in cultivation, from the commodity property of mycelial growth situation, growth cycle, fructification properties and characteristicses, biological efficiency and mushroom
Etc. aspect be analyzed, as a result show：It is feasible using Chinese chestnut bud husk and Chinese chestnut leaf Xinbao mushroom culturing, individually with Chinese chestnut
Luxuriant shell is for carbon source material or adds the materials such as appropriate straw, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits in Chinese chestnut bud husk,
Obtain preferable cultivation effect.But the tannin in Chinese chestnut bud husk is more, then when carrying out cultivating flat mushroom and elegant precious mushroom, training
Foster yield is not high, and luxuriant shell size must be suitable, and not enough, easy breed bacteria is too big easily to stab for the permeability of too little culture medium
Broken plastic foil, causes humiture not enough, affects the yield of edible mushroom, and test consumption above is less, and is only to make
For the compost of pleurotus eryngii, so Chinese chestnut bud husk must be done into the culture demand that further processing meets multiple edible mushroom, allow
Chinese chestnut bud husk is reasonably utilized, and prevents the wasting of resources, reduces production cost, improves the economic and social benefits.
The content of the invention
The object of the invention is exactly to make up the defect of prior art, there is provided a kind of cultural method of selenium-enriched edible mushroom and its
Cultural method.
The present invention is achieved by the following technical solutions：
A kind of cultural method of selenium-enriched edible mushroom, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30
Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity
For one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Mix and add appropriate deionized water after dissolving, be sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h,
Separation of solid and liquid, it is standby after filter residue is dried, wood chip, filter residue, remaining Chinese chestnut bud husk are soaked in respectively in 3% limewash
24h, is processed, primary fermentation 18 with Chinese chestnut bud husk extract, gypsum, selenium yeast, brewex's grains after taking-up is drained according to half sponge process
My god, turning 4 times altogether, then overlay film fermentation, keeps 10h at 60 DEG C, is kept for 4 days at 50 DEG C, and containing for compost is made after fermentation ends
Between 60-65%, pH value is 7-7.5 to the water yield；
The formula of the compost is according to mass percent proportioning：Chinese chestnut bud husk 54-80%, wood chip 16-20%, brewex's grains 15-
18%th, selenium yeast 2-3%, gypsum 1-1.2%, appropriate deionized water；
B, dissolve the chitosan in the acetum that pH value is 2, the shitosan vinegar that mass concentration is 2% is obtained after ultrasonic dissolution
Acid solution, the calcium nitrate solution of 0.5mol/L is added in chitosan-acetic acid solution, wherein shitosan and calcium nitrate according to rubbing
You compare 7:3 mixing, add the disodium phosphate soln of the isopyknic 0.3mol/L of calcium nitrate solution under electric stirring, treat solution
It is 10 that pH value is adjusted after being thoroughly mixed, and after stirring 1-2h sinking 12h is stood, and is filtered, and precipitate with deionized water is washed to neutrality,
Dry to constant weight at 50 DEG C in vacuum drying chamber；
C, by aurantiin, lentinan and AVM according to mass ratio 6：5：0.3 is added to dissolving in ethanol stirs, then
With 1:48 mass ratio mixes with the product prepared in step b, electric stirring 1-1.5h after ultrasonic agitation 30min, and heating is removed
Solvent, then according to g：Ml is with 1:1.5 add deionized water to continue electric stirring 1-1.5h, the thing prepared with step a after being dried
Matter is according to mass ratio 1:22 mixing and stirrings, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side dress
Side is pressed, and elasticity is suitable, is tightened and be placed in after sack sterilizing in high-pressure sterilizing pot, and 126 DEG C of 2-2.5h take out culture after natural cooling
Bag, according still further to sterile working inoculation, inoculum concentration covers charge level and is advisable with bacterial classification；
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and after the long purseful of mycelia mushroom house is produced；
E, select water quality it is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 4-6% peat soil, the river sand of 1-1.5%
With the coconut palm chaff mixing and stirring of 5-8%, then add 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% it is many
Bacterium spirit, is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, and the humidity for keeping Nutrition Soil is 45-55%, then by step d
In bacterium bag carry out vallum earthing, the condition that needs when then according to edible mushroom controls suitable humiture and ventilation condition
.
It is an advantage of the invention that：Chinese chestnut bud husk is spherical, the close involucre being needled in Castanea mollissima Nut outside, due to edible mushroom
Become adult come edible good protein by decomposing, converting the organic matter for going out of use, and Chinese chestnut bud husk contains carbon, nitrogen and ore deposit
The compositions such as material, can be as the required basic nutrition of edible fungi growth development, so the present invention is by after Chinese chestnut bud husk and liquor
Chinese chestnut bud husk slag as main charcoal source material, the juice of Chinese chestnut bud husk is used as zymotic fluid, then is aided with wood chip, fishbone dust, selenium ferment
Female, brown sugar and gypsum meet the basic nutrition needed for Edible Fungi as the compost of edible mushroom, and by chemical reaction
Prepare hydroxyapatite/chitosan composite, with good water suction, water conservation and sustained release performance, can by nutrient solution and
When be slowly supplied to edible mushroom, drought-resistant and fertilizer, it is ensured that the supply and demand of its nutrition so as to send out that bacterium is fast, wire vent is more, reduces later stage fertilizer
Labour's operation of water supply, it is time saving and energy saving, it is cost-effective, and will appear from the mushroom of former base and carry out vallum plantation, cover homemade
Nutrition Soil, can pluck mushrooms and provide nutrition so as to which mushroom growing up number is more, yield height for many batches of later stage, and for Chinese chestnut bud husk
Resource huge profit reduces the pollution to environment with new approach is found, and the cultural method that the present invention is provided can significantly increase food
With the yield of bacterium and a batch number of times is adopted, and the edible fungi nutrition planted is abundant rich in multivitamin and selenium element, it is in good taste, it is former
Material wide material sources, the good needs of small investment, high efficiency, harvest meet market demand.
Specific embodiment
A kind of cultural method of selenium-enriched edible mushroom, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, at 20 DEG C
At a temperature of cultivate, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity
One-level kind；
The Mother culture based formulas are：Potato mixed juice 200g/L, agar 12g/L, sucrose 20g/L, peptone 5g/L, work
Property charcoal 0.3g/L, adds water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30min, filter
Residue, merging filtrate is gone simultaneously to add dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, be sufficiently stirred for
Appropriate deionized water is added after dissolving, is sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160g/L, Chinese chestnut bud husk 40g/L, dusty yeast 5g/L, glucose
20g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h,
Separation of solid and liquid, it is standby after filter residue is dried, wood chip, filter residue, remaining Chinese chestnut bud husk are soaked in respectively in 3% limewash
24h, is processed, primary fermentation 18 with Chinese chestnut bud husk extract, gypsum, selenium yeast, brewex's grains after taking-up is drained according to half sponge process
My god, turning 4 times altogether, then overlay film fermentation, keeps 10h at 60 DEG C, is kept for 4 days at 50 DEG C, and containing for compost is made after fermentation ends
Between 60%, pH value is 7 to the water yield；
The formula of the compost is according to mass percent proportioning：Chinese chestnut bud husk 54%, wood chip 16%, brewex's grains 15%, selenium yeast
2%th, gypsum 1%, appropriate deionized water；
B, dissolve the chitosan in the acetum that pH value is 2, the shitosan vinegar that mass concentration is 2% is obtained after ultrasonic dissolution
Acid solution, the calcium nitrate solution of 0.5mol/L is added in chitosan-acetic acid solution, wherein shitosan and calcium nitrate according to rubbing
You compare 7:3 mixing, add the disodium phosphate soln of the isopyknic 0.3mol/L of calcium nitrate solution under electric stirring, treat solution
It is 10 that pH value is adjusted after being thoroughly mixed, and after stirring 1h sinking 12h is stood, and is filtered, and precipitate with deionized water is washed to neutrality, true
Dry to constant weight at 50 DEG C in empty drying box；
C, by aurantiin, lentinan and AVM according to mass ratio 6：5：0.3 is added to dissolving in ethanol stirs, then
With 1:48 mass ratio mixes with the product prepared in step b, electric stirring 1h after ultrasonic agitation 30min, and heating removes solvent,
Then according to g：Ml is with 1:1.5 add deionized water to continue electric stirring 1h, and the material prepared with step a after being dried is according to quality
Than 1:22 mixing and stirrings, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side rim pressure, elasticity
Suitably, tighten to be placed in after sack in high-pressure sterilizing pot and sterilize, 126 DEG C of 2h take out culture bag, according still further to aseptic behaviour after natural cooling
It is inoculated with, inoculum concentration covers charge level and is advisable with bacterial classification；
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is 18
DEG C, relative air humidity is 65%, and a culture bag was turned over every 7 days, and after the long purseful of mycelia mushroom house is produced；
E, select that water quality is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 4% peat soil, 1% river sand and 5%
Coconut palm chaff mixing and stirring, then adds 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% carbendazim, mixes
Conjunction stirs into Nutrition Soil, and overlay film boring cover 2 days, and the humidity for keeping Nutrition Soil is 45%, then carries out the bacterium bag in step d
Vallum earthing, the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
The upgrowth situation of hypha of edible fungus is compared in order to embody Chinese chestnut bud husk, according to following formula different cultures are prepared
Material is cultivated pleurotus eryngii, and to the indices of growth course observation comparison is carried out
A Chinese chestnut bud husks 54%, wood chip 16%, brewex's grains 15%, selenium yeast 2%, gypsum 1%
B glucose 54%, wood chip 16%, brewex's grains 15%, selenium yeast 2%, gypsum 1%
C Chinese chestnut bud husks 70%, brewex's grains 15%, selenium yeast 2%, gypsum 1%
D glucose 70%, brewex's grains 15%, selenium yeast 2%, gypsum 1%
The mycelial growth situation of control
Long speed（mm/d）Mycelial density mycelia thickness mycelia color and luster
A 4.19 +++ ++ it is relatively thick dense white
B 3.87 ++++thin dense white
C 4.03 ++++thick dense white
D 3.78 +++ it is relatively thin white
"+" number represents mycelial density, and "+" represents that density is bigger
Growth cycle compares
There is former base（d）Fructification grows up to（d）Growth cycle（d）
A 7 7 81
B 8 8.5 88
C 7 7.5 86
D 9 9 93
The properties and characteristicses of each formula fructification
The mono- mushroom weight/g unit weights/g/mL of stem diameter/cm stems length/cm
A 3.6 11.35 54.54 0.75
B 3.4 10.79 41.69 0.84
C 3.4 11.11 45.45 0.80
D 3.1 10.21 39.12 0.87
Claims (1)
1. a kind of cultural method of selenium-enriched edible mushroom, it is characterised in that including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put in pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in while hot in test tube, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30
Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then Jing expands and numerous is to desired quantity
For one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Mix and add appropriate deionized water after dissolving, be sub-packed in while hot in container, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it was added in deionized water and decocts 2h,
Separation of solid and liquid, it is standby after filter residue is dried, wood chip, filter residue, remaining Chinese chestnut bud husk are soaked in respectively in 3% limewash
24h, is processed with Chinese chestnut bud husk extract, gypsum, selenium yeast, fishbone dust and brown sugar after taking-up is drained according to half sponge process, front
Fermentation 18 days, turning 4 times altogether, then overlay film fermentation, keeps 10h at 60 DEG C, is kept for 4 days at 50 DEG C, and culture is made after fermentation ends
Between 60-65%, pH value is 7-7.5 to the water content of material；
The formula of the compost is according to mass percent proportioning：Chinese chestnut bud husk 68-80%, wood chip 16-20%, fishbone dust 2-
3%th, selenium yeast 2-3%, brown sugar 1-1.5%, gypsum 1-1.2%, appropriate deionized water；
B, dissolve the chitosan in the acetum that pH value is 2, the shitosan vinegar that mass concentration is 2% is obtained after ultrasonic dissolution
Acid solution, the calcium nitrate solution of 0.5mol/L is added in chitosan-acetic acid solution, wherein shitosan and calcium nitrate according to rubbing
You compare 7:3 mixing, add the disodium phosphate soln of the isopyknic 0.3mol/L of calcium nitrate solution under electric stirring, treat solution
It is 10 that pH value is adjusted after being thoroughly mixed, and after stirring 1-2h sinking 12h is stood, and is filtered, and precipitate with deionized water is washed to neutrality,
Dry to constant weight at 50 DEG C in vacuum drying chamber；
C, by aurantiin, lentinan and AVM according to mass ratio 6：5：0.3 is added to dissolving in ethanol stirs, then
With 1:48 mass ratio mixes with the product prepared in step b, electric stirring 1-1.5h after ultrasonic agitation 30min, and heating is removed
Solvent, then according to g：Ml is with 1:1.5 add deionized water to continue electric stirring 1-1.5h, the thing prepared with step a after being dried
Matter is according to mass ratio 1:22 mixing and stirrings, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side dress
Side is pressed, and elasticity is suitable, is tightened and be placed in after sack sterilizing in high-pressure sterilizing pot, and 126 DEG C of 2-2.5h take out culture after natural cooling
Bag, according still further to sterile working inoculation, inoculum concentration covers charge level and is advisable with bacterial classification；
D, the culturing room for postvaccinal culture bag being placed into into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and after the long purseful of mycelia mushroom house is produced；
E, select water quality it is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 4-6% peat soil, the river sand of 1-1.5%
With the coconut palm chaff mixing and stirring of 5-8%, then add 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% it is many
Bacterium spirit, is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, and the humidity for keeping Nutrition Soil is 45-55%, then by step d
In bacterium bag carry out vallum earthing, the condition that needs when then according to edible mushroom controls suitable humiture and ventilation condition
.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184541A (en) * | 2018-03-08 | 2018-06-22 | 山西省农业科学院农业资源与经济研究所 | The production method of Radix Astragali functional edible mushroom |
| CN108522146A (en) * | 2018-05-31 | 2018-09-14 | 安徽丰尚生态农业科技有限公司 | A kind of mushroom plantation special nutrient soil |
| CN108782014A (en) * | 2018-05-31 | 2018-11-13 | 安徽丰尚生态农业科技有限公司 | A kind of preparation method of mushroom plantation Nutrition Soil |
| CN109699394A (en) * | 2019-03-11 | 2019-05-03 | 西南科技大学 | A method of vertical stripe Xylaria sp. fungus fructification is produced using fluid nutrient medium |
| CN111972213A (en) * | 2020-09-09 | 2020-11-24 | 岳西县思远生态农业有限公司 | Black fungus cultivation method |
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| CN105532257A (en) * | 2015-12-07 | 2016-05-04 | 武文礼 | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105532257A (en) * | 2015-12-07 | 2016-05-04 | 武文礼 | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184541A (en) * | 2018-03-08 | 2018-06-22 | 山西省农业科学院农业资源与经济研究所 | The production method of Radix Astragali functional edible mushroom |
| CN108522146A (en) * | 2018-05-31 | 2018-09-14 | 安徽丰尚生态农业科技有限公司 | A kind of mushroom plantation special nutrient soil |
| CN108782014A (en) * | 2018-05-31 | 2018-11-13 | 安徽丰尚生态农业科技有限公司 | A kind of preparation method of mushroom plantation Nutrition Soil |
| CN109699394A (en) * | 2019-03-11 | 2019-05-03 | 西南科技大学 | A method of vertical stripe Xylaria sp. fungus fructification is produced using fluid nutrient medium |
| CN111972213A (en) * | 2020-09-09 | 2020-11-24 | 岳西县思远生态农业有限公司 | Black fungus cultivation method |
| CN111972213B (en) * | 2020-09-09 | 2022-04-15 | 岳西县思远生态农业有限公司 | Black fungus cultivation method |
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