CN106489532A - A kind of cultivating method for edible fungi of low production cost - Google Patents

A kind of cultivating method for edible fungi of low production cost Download PDF

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Publication number
CN106489532A
CN106489532A CN201611044371.5A CN201611044371A CN106489532A CN 106489532 A CN106489532 A CN 106489532A CN 201611044371 A CN201611044371 A CN 201611044371A CN 106489532 A CN106489532 A CN 106489532A
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culture
deionized water
chinese chestnut
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chestnut bud
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唐修志
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Yingshang County Duo Tang Lake Of Modern Agricultural Science And Technology Co Ltd
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Yingshang County Duo Tang Lake Of Modern Agricultural Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of cultivating method for edible fungi of low production cost, preparation method of the present invention is easy to learn, is easy to promote, and raw material sources are extensive, with low cost, beneficial to batch production, formula is rationally, the edible fungi nutrition that turns out enriches, and with certain health value, meets the market demand.

Description

A kind of cultivating method for edible fungi of low production cost
Technical field
A kind of the present invention relates to fungus growing technique field, more particularly to cultivating method for edible fungi of low production cost.
Background technology
With the progress of science and technology, mushroom industry fast development, the cultivation two of edible mushroom are constantly increasing, thus right The demand of culturing raw material increases increasingly.In edible mushroom substituting stuff cultivation technology, traditional cultivation raw material mainly have cottonseed, bagasse, Weed tree sawdust, crop material etc..With people increasingly mature to the technology of these raw material comprehensive utilization, the purposes of these raw materials More extensive, such as wood chip is used for processing synthetic plate, and bagasse is used for papermaking etc., this provides for improved Edible Fungi cost, nothing The pressure of Edible Fungi enterprise is increased in shape, therefore research and development novel edible fungus culturing raw material, reduce into product cost, Increase economic efficiency, have great importance.China is the big country of Chinese chestnut plantation, in Chinese chestnut growing area, annual harvesting Chinese chestnut it Substantial amounts of leftover bits and pieces Chinese chestnut bud husk can all be produced afterwards.Current comprehensive utilizating research and exploitation of the people to Chinese chestnut bud husk, mostly The aspects such as solid shape carbon production, tannin extract and nature strength is all concentrated on, as Chinese chestnut bud husk contains carbon, nitrogen and mineral matter etc. Composition, it is possible to provide basic nutrition necessary to edible fungi growth development.
Qin Baoshan exists《The test of Chinese chestnut bud husk Xinbao mushroom culturing》With Chinese chestnut bud husk as main carbon source material in one text, with rice Grass, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits etc. for auxiliary carbon source material, with rice bran as nitrogen source, arrange 10 process carry out apricot Abalone mushroom experiment in cultivation, from the commodity property of mycelial growth situation, growth cycle, fructification properties and characteristicses, biological efficiency and mushroom Etc. aspect be analyzed, as a result show:It is feasible using Chinese chestnut bud husk and Chinese chestnut leaf Xinbao mushroom culturing, individually with Chinese chestnut Luxuriant shell adds for carbon source material or in Chinese chestnut bud husk the materials such as appropriate straw, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits, Obtain preferable cultivation effect.But the tannin in Chinese chestnut bud husk is more, then when carrying out cultivating flat mushroom and elegant precious mushroom, training Foster yield is not high, and luxuriant shell size is required suitable, and the permeability of too little culture medium not enough, easily stab greatly very much by easy breed bacteria Broken plastic foil, causes humiture not enough, affects the yield of edible mushroom, and test consumption above is less, and is only to make For the compost of pleurotus eryngii, so Chinese chestnut bud husk must be done the culture demand that further processing meets multiple edible mushroom, allow Chinese chestnut bud husk is reasonably utilized, and prevents the wasting of resources, reduces production cost, improves the economic and social benefits.
Content of the invention
The object of the invention is exactly the defect in order to make up prior art, there is provided a kind of edible fungus culturing side of low production cost Method.
The present invention is achieved by the following technical solutions:
A kind of cultivating method for edible fungi of low production cost, including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes are cut into slices, the peeling section of rotten banana is put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, filters off residue, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot and sterilize in 121 DEG C 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30 Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then through expanding numerous to desired quantity be For one-level kind;
The Mother culture based formulas are:Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min, Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot and sterilize in 121 DEG C 40min, is cooled to standby after room temperature;Liquid is inoculated into first class inoculum on aseptic operating platform according to 0.2mm 0.2mm sizes In culture medium, cultivated with 180r/min in the shaking table for being placed in 25 DEG C, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/ L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Banana Leaf is cleaned post-drying, it is standby to be cut into 10cm sizes, Chinese chestnut bud husk was crushed 60 mesh sieves, compares one according to weight It is divided into two, a copy of it is added to decoction 2h in deionized water, and separation of solid and liquid, with remaining Chinese chestnut bud husk, perfume (or spice) after filter residue is dried Any of several broadleaf plants leaf is soaked in 24h in 3% limewash respectively, takes out after draining and Chinese chestnut bud husk extract, wheat, gypsum are according to secondary The process of ferment method, primary fermentation 18 days, turning 4 times altogether, then overlay film fermentation keep 10h at 60 DEG C, are kept for 4 days at 50 DEG C, fermentation knot Make the water content of compost between 60-65% after beam, pH value is 7-7.5;
The formula of the compost according to mass percent proportioning is:Chinese chestnut bud husk 70-80%, Banana Leaf 20-26%, wheat 3- 4%th, gypsum 1-1.3%, appropriate deionized water;
B, will after cotton leftovers ungrease treatment clean dry, be ground into 200 mesh and be added in container, add deionized water water-bath add Heat to 60 DEG C and is passed through nitrogen, is stirred continuously acrylic acid, potassium peroxydisulfate and N, N- di-2-ethylhexylphosphine oxide third that lower addition degree of neutralization is 72% Acrylamide, wherein acrylic acid spend leftover bits and pieces proportioning adding for 6-9ml/g, potassium peroxydisulfate and N, N- methylene-bisacrylamide with face Enter 1.3% and 0.12%, reaction 2-2.5h at 75-85 DEG C that amount is acrylic acid quality, after reaction terminates, product deionized water Wash 3 times and washed once with ethanol, crush after drying standby;
C, by 0.4-0.6ppm vitamin Ks, the mannitol of 0.3-0.6g/L, 0.2-0.3g/L Organic Seleniums, 0.1-0.2g/L dichloros The humic acid of sodium isocyanurate, 2-3g/L shitosans and 5-6g/L is added in the deionized water of 1L and stirs, and adds step b The product of middle preparation ultrasonic agitation 40-50min under 500W, ultrasound are filtered after terminating, are dried, then according to mass ratio 1:20 Hes Product mixing and stirring prepared by step a, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side rim Pressure, elasticity are suitable, be placed in high-pressure sterilizing pot and sterilize after tightening sack, 126 DEG C of 2-2.5h, take out culture after natural cooling Bag, is inoculated with according still further to sterile working, and inoculum concentration covers charge level with bacterial classification and is advisable;
D, the culturing room for postvaccinal culture bag being placed into drying, being cleaned, is divulged information carry out half-light culture, and temperature control is in 18- 28 DEG C, relative air humidity is 65-75%, turned over a culture bag every 7-10 days, produces mushroom house after the long purseful of mycelia;
E, select that water quality is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add the coconut palm chaff of 2-3%, the perlite of 3-5% and The peat soil mixing and stirring of 5-8%, then add 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% many Bacterium spirit, is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, and the humidity for keeping Nutrition Soil is 45-55%, then by step d In bacterium bag carry out vallum earthing, the condition that needs when then according to edible mushroom controls suitable humiture and ventilation condition ?.
It is an advantage of the invention that:Chinese chestnut bud husk is spherical outside Castanea mollissima Nut, the close involucre being needled, due to edible mushroom Become adult come edible good protein by decomposing, converting the organic matter for going out of use, and Chinese chestnut bud husk contains carbon, nitrogen and ore deposit The compositions such as material, can be as basic nutrition necessary to edible fungi growth development, so after the present invention is by Chinese chestnut bud husk and liquor Chinese chestnut bud husk slag as main charcoal source material, the juice of Chinese chestnut bud husk is used as zymotic fluid, then is aided with Banana Leaf, wheat and gypsum As the compost of edible mushroom, and cotton leftovers water-absorbing resin is prepared using chemical reaction, with good water suction, guarantor Water and sustained release performance, nutrient solution can be supplied to edible mushroom, drought-resistant and fertilizer in time, it is ensured that the supply and demand of its nutrition so as to send out bacterium Hurry up, wire vent is more, the labour for reducing the water supply of later stage fertilizer operates, time saving and energy saving, cost-effective, and will appear from the mushroom of former base Row vallum is planted, and covers homemade Nutrition Soil, can provide nutrition for many batches of harvesting mushrooms of later stage so as to which mushroom growing up number is more, product Amount is high, and the resource huge profit for Chinese chestnut bud husk is simple with new approach, pollution of the minimizing to environment, preparation method of the present invention is found Single easy to learn, it to be easy to promote, raw material sources are extensively, with low cost, and beneficial to batch production, formula is reasonable, the edible fungi nutrition that turns out Abundant, with certain health value, meet the market demand.
Specific embodiment
A kind of cultivating method for edible fungi of low production cost, including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes are cut into slices, the peeling section of rotten banana is put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, filters off residue, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot and sterilize in 121 DEG C 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, at 20 DEG C At a temperature of cultivate, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding One-level kind;
The Mother culture based formulas are:Potato mixed juice 200g/L, agar 12g/L, sucrose 20g/L, peptone 5g/L, work Property charcoal 0.3g/L, adds water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30min, filter Residue, merging filtrate is gone simultaneously to add dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, be sufficiently stirred for Appropriate deionized water is added after dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot and sterilize in 121 DEG C 40min, is cooled to standby after room temperature;Liquid is inoculated into first class inoculum on aseptic operating platform according to 0.2mm 0.2mm sizes In culture medium, cultivated with 180r/min in the shaking table for being placed in 25 DEG C, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160g/L, Chinese chestnut bud husk 40g/L, dusty yeast 5g/L, glucose 20g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Banana Leaf is cleaned post-drying, it is standby to be cut into 10cm sizes, Chinese chestnut bud husk was crushed 60 mesh sieves, compares one according to weight It is divided into two, a copy of it is added to decoction 2h in deionized water, and separation of solid and liquid, with remaining Chinese chestnut bud husk, perfume (or spice) after filter residue is dried Any of several broadleaf plants leaf is soaked in 24h in 3% limewash respectively, takes out after draining and Chinese chestnut bud husk extract, wheat, gypsum are according to secondary The process of ferment method, primary fermentation 18 days, turning 4 times altogether, then overlay film fermentation keep 10h at 60 DEG C, are kept for 4 days at 50 DEG C, fermentation knot Make the water content of compost between 60% after beam, pH value is 7;
The formula of the compost according to mass percent proportioning is:Chinese chestnut bud husk 70%, Banana Leaf 20%, wheat 3%, gypsum 1%, Appropriate deionized water;
B, will after cotton leftovers ungrease treatment clean dry, be ground into 200 mesh and be added in container, add deionized water water-bath add Heat to 60 DEG C and is passed through nitrogen, is stirred continuously acrylic acid, potassium peroxydisulfate and N, N di-2-ethylhexylphosphine oxide third that lower addition degree of neutralization is 72% Acrylamide, wherein acrylic acid spend leftover bits and pieces proportioning for 6ml/g with face, the addition of potassium peroxydisulfate and N, N methylene-bisacrylamide 1.3% and 0.12% for acrylic acid quality being measured, 2h being reacted at 75 DEG C, after reaction terminates, product is washed with deionized water 3 times and is used in combination Ethanol washed once, and crush standby after drying;
C, by 0.4ppm vitamin Ks, the mannitol of 0.3g/L, 0.2g/L Organic Seleniums, 0.1g/L sodium dichloro cyanurates, 2g/L shells The humic acid of glycan and 5g/L is added in the deionized water of 1L and stirs, and adds the product prepared in step b under 500W Ultrasonic agitation 40min, ultrasound are filtered after terminating, are dried, then according to mass ratio 1:20 and step a prepare product mix Uniformly, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side rim pressure, elasticity are suitable, tighten sack After be placed in high-pressure sterilizing pot sterilize, 126 DEG C of 2h, after natural cooling take out culture bag, according still further to sterile working be inoculated with, inoculum concentration Charge level is covered with bacterial classification to be advisable;
D, postvaccinal culture bag is placed into dry, cleaning, the culturing room of ventilation carry out half-light culture, temperature control is 18 DEG C, relative air humidity is 65%, turned over a culture bag every 7 days, produces mushroom house after the long purseful of mycelia;
E, select that water quality is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add 2% coconut palm chaff, 3% perlite and 5% Peat soil mixing and stirring, then add 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% carbendazim, It is mixed into Nutrition Soil, and overlay film boring cover 2 days, the humidity for keeping Nutrition Soil is 45%, then enters the bacterium bag in step d Row vallum earthing, the condition needed when then according to edible mushroom control suitable humiture and ventilation condition.
The upgrowth situation of hypha of edible fungus is compared in order to embody Chinese chestnut bud husk, different cultures are prepared according to following formula Material is cultivated to pleurotus eryngii, carries out observation comparison to the indices of growth course
A Chinese chestnut bud husks 70%, Banana Leaf 20%, wheat 3%, gypsum 1%
B glucose 70%, Banana Leaf 20%, wheat 3%, gypsum 1%
C Chinese chestnut bud husks 94%, wheat 3%, gypsum 1%
D glucose 94%, wheat 3%, gypsum 1%
The mycelial growth situation of control
Long speed(mm/d)Mycelial density mycelia thickness mycelia color and luster
A 4.18 +++ ++ denseer whiter
B 3.95 ++++carefully dense white
C 3.99 ++++slightly dense white
D 3.84 +++ relatively thin white
"+" number represents mycelial density, and "+" represents that density is bigger
Growth cycle compares
There is former base(d)Fructification grows up to(d)Growth cycle(d)
A 7 7 81
B 8 8.5 88
C 7 7.5 86
D 9 9 91
The properties and characteristicses of each formula fructification
The mono- mushroom weight/g unit weights/g/mL of stem diameter/cm stems length/cm
A 3.8 11.24 53.07 0.75
B 3.6 10.92 42.63 0.84
C 3.7 11.05 46.54 0.78
D 3.4 10.38 39.53 0.85

Claims (1)

1. a kind of cultivating method for edible fungi of low production cost, it is characterised in that including step in detail below:
(1)The separation and culture of edible mushroom parent species:
Peeling potatoes are cut into slices, the peeling section of rotten banana is put in pot, according to mass volume ratio 1g:5ml adds deionized water Potato is boiled to thoroughly well cooked but not mushy, filters off residue, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot and sterilize in 121 DEG C 30min, takes out after cooling and is put into inclined-plane, then edible hyphostroma is seeded on culture medium in aseptic operating platform, in 20-30 Cultivate at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then through expanding numerous to desired quantity be For one-level kind;
The Mother culture based formulas are:Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL;
(2)The preparation and culture of liquid strain culture medium:
Chinese chestnut bud husk pulverizer was crushed 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min, Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot and sterilize in 121 DEG C 40min, is cooled to standby after room temperature;Liquid is inoculated into first class inoculum on aseptic operating platform according to 0.2mm 0.2mm sizes In culture medium, cultivated with 180r/min in the shaking table for being placed in 25 DEG C, when mycelium pellet covers with 80% culture medium, you can Solid culture medium is inoculated with;
The Liquid Culture based formulas are:Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/ L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL;
(3)Cultivation fruiting:
A, Banana Leaf is cleaned post-drying, it is standby to be cut into 10cm sizes, Chinese chestnut bud husk was crushed 60 mesh sieves, compares one according to weight It is divided into two, a copy of it is added to decoction 2h in deionized water, and separation of solid and liquid, with remaining Chinese chestnut bud husk, perfume (or spice) after filter residue is dried Any of several broadleaf plants leaf is soaked in 24h in 3% limewash respectively, takes out after draining and Chinese chestnut bud husk extract, wheat, gypsum are according to secondary The process of ferment method, primary fermentation 18 days, turning 4 times altogether, then overlay film fermentation keep 10h at 60 DEG C, are kept for 4 days at 50 DEG C, fermentation knot Make the water content of compost between 60-65% after beam, pH value is 7-7.5;
The formula of the compost according to mass percent proportioning is:Chinese chestnut bud husk 70-80%, Banana Leaf 20-26%, wheat 3- 4%th, gypsum 1-1.3%, appropriate deionized water;
B, will after cotton leftovers ungrease treatment clean dry, be ground into 200 mesh and be added in container, add deionized water water-bath add Heat to 60 DEG C and is passed through nitrogen, is stirred continuously acrylic acid, potassium peroxydisulfate and N, N- di-2-ethylhexylphosphine oxide third that lower addition degree of neutralization is 72% Acrylamide, wherein acrylic acid spend leftover bits and pieces proportioning adding for 6-9ml/g, potassium peroxydisulfate and N, N- methylene-bisacrylamide with face Enter 1.3% and 0.12%, reaction 2-2.5h at 75-85 DEG C that amount is acrylic acid quality, after reaction terminates, product deionized water Wash 3 times and washed once with ethanol, crush after drying standby;
C, by 0.4-0.6ppm vitamin Ks, the mannitol of 0.3-0.6g/L, 0.2-0.3g/L Organic Seleniums, 0.1-0.2g/L dichloros The humic acid of sodium isocyanurate, 2-3g/L shitosans and 5-6g/L is added in the deionized water of 1L and stirs, and adds step b The product of middle preparation ultrasonic agitation 40-50min under 500W, ultrasound are filtered after terminating, are dried, then according to mass ratio 1:20 Hes Product mixing and stirring prepared by step a, then using the packed mixed culture material of polypropylene plastics of appropriate gauge, side rim Pressure, elasticity are suitable, be placed in high-pressure sterilizing pot and sterilize after tightening sack, 126 DEG C of 2-2.5h, take out culture after natural cooling Bag, is inoculated with according still further to sterile working, and inoculum concentration covers charge level with bacterial classification and is advisable;
D, the culturing room for postvaccinal culture bag being placed into drying, being cleaned, is divulged information carry out half-light culture, and temperature control is in 18- 28 DEG C, relative air humidity is 65-75%, turned over a culture bag every 7-10 days, produces mushroom house after the long purseful of mycelia;
E, select that water quality is good, quality is loose, without miscellaneous worm's ovum 10% zinc-rich soil, add the coconut palm chaff of 2-3%, the perlite of 3-5% and The peat soil mixing and stirring of 5-8%, then add 0.1% urea, 0.1% amino acid, 1.5% lime and 0.1% many Bacterium spirit, is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, and the humidity for keeping Nutrition Soil is 45-55%, then by step d In bacterium bag carry out vallum earthing, the condition that needs when then according to edible mushroom controls suitable humiture and ventilation condition ?.
CN201611044371.5A 2016-11-24 2016-11-24 A kind of cultivating method for edible fungi of low production cost Pending CN106489532A (en)

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Cited By (4)

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CN108812054A (en) * 2018-05-30 2018-11-16 江苏圣福来生态农业有限公司 A method of cultivation Hericium erinaceus
CN111448943A (en) * 2020-04-11 2020-07-28 达州市农业科学研究院 Formula of liquid strain culture medium for fresh-colored lactarius and preparation method of liquid strain
CN112703967A (en) * 2020-12-07 2021-04-27 贵州大学 Method for preparing wood rot fungus liquid strain by using yellow serofluid and bean dregs
CN113812304A (en) * 2021-11-05 2021-12-21 桂东森宜食用菌发展有限公司 Edible mushroom inoculation method

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CN105493885A (en) * 2015-11-24 2016-04-20 金乡县联盛菌业科技有限公司 Culture method of black termitomyces albuminosus
CN105393796A (en) * 2015-11-30 2016-03-16 全椒县香妃农业专业合作社 Nutrition summer mushroom plantation method
CN105532257A (en) * 2015-12-07 2016-05-04 武文礼 Pleurotusostreatus and pleurotus eryngii high-yield cultivation method

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CN108812054A (en) * 2018-05-30 2018-11-16 江苏圣福来生态农业有限公司 A method of cultivation Hericium erinaceus
CN108812054B (en) * 2018-05-30 2020-06-09 江苏圣福来生态农业有限公司 Method for cultivating hericium erinaceus
CN111448943A (en) * 2020-04-11 2020-07-28 达州市农业科学研究院 Formula of liquid strain culture medium for fresh-colored lactarius and preparation method of liquid strain
CN112703967A (en) * 2020-12-07 2021-04-27 贵州大学 Method for preparing wood rot fungus liquid strain by using yellow serofluid and bean dregs
CN113812304A (en) * 2021-11-05 2021-12-21 桂东森宜食用菌发展有限公司 Edible mushroom inoculation method

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