CN1557122A - Schizophyllum commune artificial cultivation and breeding technology - Google Patents

Schizophyllum commune artificial cultivation and breeding technology Download PDF

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Publication number
CN1557122A
CN1557122A CNA200410021821XA CN200410021821A CN1557122A CN 1557122 A CN1557122 A CN 1557122A CN A200410021821X A CNA200410021821X A CN A200410021821XA CN 200410021821 A CN200410021821 A CN 200410021821A CN 1557122 A CN1557122 A CN 1557122A
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inoculation
bag
cultivate
artificial cultivation
mycelia
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CNA200410021821XA
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CN100399876C (en
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段会昌
余家贵
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TENGCHONG COUNTY ZHONGHE TOWNSHIP PEASANT CULTURE AND TECHNIQUE SCHOOL
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TENGCHONG COUNTY ZHONGHE TOWNSHIP PEASANT CULTURE AND TECHNIQUE SCHOOL
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Priority to CNB200410021821XA priority Critical patent/CN100399876C/en
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Abstract

The artificially cultivation and propagation technology of Schizophyllum commune Fr. includes tissue culture to purify and obtain wild strain of Schizophyllum commune Fr., artificially cultivation in combined culture material, domesticating, propagation, tissue separation and other steps. During the cultivation, the temperature, humidity, air flow and scattered sunlight are artificially controlled so as to produce large, high quality and high yield Schizophyllum commune Fr.

Description

The artificial cultivation raising technology of white ginseng bacterium
Technical field
The invention belongs to artificial cultivation edible mushroom technical field, the artificial cultivation raising technology of especially a kind of white ginseng bacterium.
Background technology
White ginseng bacterium has another name called into the load bavin, belongs to Basidiomycotina, Hymenomycetes on the taxonomy, Agaricales, schizophyllum commune section.Wild white ginseng bacterium existence growth conditions is to external world selected very tight, and very strong seasonality is arranged again, and it can be grown in 8 ℃~30 ℃ scopes to the adaptability broad of temperature, and is optimum temperature with 16 ℃~24 ℃; It is tighter to the requirement of humidity, reach 85% when above as air humidity, white ginseng fruit body only needs seven day time just can finish from breaking up maturation, if air humidity is lower than at 65% o'clock, its fruit body just i.e. dehydration is dead, though rapid moisturizing can not also be given birth to after finding its dehydration, joins bacterium in vain and likes astigmatic illumination with respect to other bacterium, the bright air of loving the new is joined the bacterium happiness in vain and is grown on the trunk that birch, nanmu, oak and Fagaceae deciduous species begin poor rotten stub and lodging.All former address of once joining bacterium in vain of growing can not grow white ginseng bacterium more for the second time.Wild white ginseng bacterium mostly is single-lobe, answer lobe less, the length of lobe is many at 1~2cm, its quality softness, delicious flavour also has self unique fragrance flavor, it is rich in several amino acids and the trace element that needed by human body is wanted, it is a kind of rich nutrition edible fungus, be again medicinal fungus simultaneously, often edible or soaked drink has and clears liver and improve vision, the medical function of stomach-invigorating intestine-moisturizing, also to gynaecology, infantile night sweat, neurasthenia, dizzy tinnitus, diseases such as constipation hemorrhoid have certain curative effect, gather and sell so always be used as the rare product of mountain delicacy by the mountain people.Modern science is known, joins bacterium in vain enhancing immune function of human body, cancer-resisting are had certain curative effect.But because white ginseng bacterium is a kind of wild bacterium, piece shape is less, piece maturation time inconsistent again, when traditional collecting method is taken a lot of work, far can not satisfy people's demand, if can carry out artificial introducing and planting and join in vain, and under the regulation and control of artificial science, improve the quality, increase output, will obtain excellent economic well-being of workers and staff and good social benefit.
By retrieval, existing a application for a patent for invention, CN00112649.0, name are called " artificial culture schizophyllum sporophores and cultural method thereof ", have proposed manually to introduce a fine variety the method for cultivating white ginseng bacterium, but exist to foretell shortcoming:
1, rise at the 17 word of first page of last column of this patent application specification, promptly " in test tube, add medium access bacterial strain and seal sterilization; pressure is at 1.5 kilograms/square centimeter; kept 30 minutes; treat to take out when pressure drops to zero and place slant culture; cultivation temperature is 20~28 ℃; 8~12 days, mycelia is covered with the inclined-plane and promptly can be used for breeding original seed "; it is earlier to inserting bacterial strain on the medium in the test tube, promptly is bacterial classification, and assorted bacterium again goes to go out; the result is that the bacterial classification that is connected on the medium will be dead simultaneously under sterilising conditions together in company with assorted bacterium, and it can not grow mycelia and use for breeding original seed on the test tube slant medium.
2, joining bacterium in vain is typical good gas fungi, and its growth be unable to do without element sulphur, if but the consumption of sulphur is excessive, and the sulfur content that is decomposited by bacterium is just many, and free sulphur very easily forms the hydrogen sulfide gas of volume and suppresses the growth of bacterial strain, even kills bacterial strain.And used land plaster all accounts for 1~2% in four prescriptions of this patent application, and the consumption that seems is too much, is unprofitable to cultivation, the growth of white ginseng bacterium.
3, add earlier in this patent application behind the urea again under high pressure or normal pressure, 100 ℃ of temperature and sterilize, and the urea inefficacy that under this sterilising conditions, has been decomposed.
4, use corn flour 10~20% in this patent application, its consumption is too high, easily causes the infection of assorted bacterium; And the mineral matter that this prescription added is wide in variety, and element repeats.
Summary of the invention
The invention provides the artificial cultivation raising technology of a kind of white ginseng bacterium, it is according to the required living environment of white ginseng bacteria growing feature, by artificial collocation to combined ingredient, domestication is bred through preferred and the isolated wild stable bacterial classification of tissue isolation and is supplied artificial cultivation, and artificially control temperature, humidity, circulation of air and light scattering, thereby it is bigger to produce plant type, higher and the white ginseng bacterium that can stable high yield of internal substance.
The present invention is achieved in that open-air preferred white ginseng bacterial classification, purifying through tissue isolation obtains wild strain (Schizophyllum commune Fr.), adopts PDA, VB agar protein culture medium to pack in the test tube, inserts bacterial strain behind autoclave sterilization, cultivate female kind, also claim the one-level kind; The wheat that boils or iblet are placed in bag or the bottle, high-temperature and high-pressure conditions down the inoculation of sterilization back female plant and cultivate original seed, also claim the secondary kind; By artificial batching, the inoculation original seed and cultivate three grades of kinds, also claim the extension of cultivated species or scale to produce kind, move into big grid frame sign indicating number descendant and cultivate the production and sales type for control temperature, humidity, circulation of air and light scattering and join in vain, can gather, process, sell.
The present invention realizes by following technical measures: by the preferred white ginseng bacterial classification in field, purifying through tissue isolation obtains wild strain, adopts PDA, VB agar protein culture medium to pack in the test tube, inserts bacterial strain behind autoclave sterilization, cultivate female kind, also claim the one-level kind; The wheat that boils or iblet are placed in bag or the bottle, high-temperature and high-pressure conditions down the inoculation of sterilization back female plant and cultivate original seed, also claim the secondary kind; By the medium of preparation, the inoculation original seed and cultivate three grades of kinds, also claim the extension of cultivated species or scale to produce kind, it is characterized in that: the prescription composition of cultivated species medium is by weight percentage
1), broad-leaved weedtree wood chip 68~80%, wheat bran 17~30%, calcium sulphate 0.5%, calcium carbonate 0.5%, sugar 1%, V B0.01%, or
2), corncob cellulose powder 50~68%, weedtree wood chip 20~28%, wheat bran 10~20%, calcium carbonate 1%, sugar 1%, or
3), rape accessory substance 50~70%, weed tree sawdust 10~20%, rice bran 10~18%, corn flour 8~10%, calcium carbonate 0.5%, superphosphate 0.5%, sugar 1%,
More than batching is 1: 1.2~1.3 mixing by material-water ratio and stirs, reaching at 50~55% o'clock and pack into the assorted bacterium that does in the bag to go out to water content handles the back and inoculates under aseptic condition, be inserted in again in the clean bag and send into culturing room, under the scattered light irradiation of 10~20 luxs, cultivate, the mycelia purseful promptly makes cultivated species, in time move in the grid frame and put on the shelf, grand style, the intact back of grand style to grid frame humidification to the humidity that is not less than 90%, normal temperature was windowed 30 minutes following every day, can grow white ginseng from the inoculation eye in 3~5 days.
As major ingredient, wheat bran or rice bran or corn flour are auxiliary material to above-mentioned cultivated species medium, add the mineral matter of trace again, V by the wood chips of the broad-leaved weedtree seeds that do not contain fat ether and aromatic oil or corncob cellulose powder or rape accessory substance B, sugar forms as additive, wherein each 50% constitutes the broad-leaved weed tree sawdust by wood chip and wood shavings, the rape accessory substance is made of rape shell 30% and rape straw powder 70%.
In the above-mentioned access strain culturing one-level kind process, under the scattered light irradiation of 10~20 luxs, cultivate, behind the mycelia full packages, mycelia is cooked alternating temperature refining kind of processing, promptly on the basis of cultivation temperature, raised or reduce 1 ℃ through 12 hours, but the highlyest be no more than 30 ℃, minimumly be not less than 16 ℃, through 2~5 days change temperature hardening bacterial classifications, for cultivate the secondary kind use or be put into preserve under 4 ℃ of temperature stand-by.
The mycelia of above-mentioned bacterial classifications at different levels need be cultivated under the scattered light irradiation of 10~20 luxs in the process that expands propagating and cultivating.
During above-mentioned inoculation one-level kind, the dislocation inoculation of the nearly two ends of the employing that in desinfection chamber, is packed in bag, with bottled employing deep layer inoculation, the inoculation back is at normal temperature and have under the light scattering condition of 10-20 lux and cultivate, and mycelia is covered with bag or full bottle and promptly gets the secondary kind.
The inoculation of above-mentioned cultivated species adopts nine of positive double pleasing to the eye or three three rows' outside of belly multiple spots to beat the cave inoculation method.
The above-mentioned bag cultivating kind that covers with mycelia moves into three-dimensional tier rack type row mushroom grid frame.
During above-mentioned grand style with bacterium bag or bacterium rod inoculation eye upper quadrant or earlier downwards the back upper quadrant give the bacterial classification moulding.
Embodiment:
Embodiment 1: make the female method of operating of planting of 1000 square metres of medium test tubes
The season of white ginseng raised growth out of office is vigorous, large stretch of to open-air preferred growth, color and luster normal, the white ginseng bacterial classification of no damage by disease and insect, to laboratory using PDA, V BSynthetic prescription is done medium, takes by weighing peeling potato 200g by weight, and back in small, broken bits is mashed after 4 to 6 layers of filtered through gauze taking juice with the 1000ml poach, adds water and make one to amount to 1000ml and place and be heated to 90 ℃ in the pot in juice; Take by weighing glucose 20g simultaneously, potassium dihydrogen phosphate 3g, compound V B10g, magnesium sulfate 1.5g, peptone 2g, agar 20g, add in the pot and stir, pack into while hot after waiting to dissolve in the test tube, will adorn the liquid test tube and pack in the pressure cooker at 117 kPas, sterilization is 45 minutes under 123 ℃ of conditions, big inclined-plane is put in taking-up after treating the nature step-down, after the desinfection chamber inoculation, 10~20 lux light scatterings have been placed on, cultivate under 20 ℃ of temperature, through 5-8 days mycelia full packages, mycelia full packages backsight temperature Change planting season situation, can heighten or reduce the test tube temperature bacterial classification is carried out alternating temperature refining kind, to strengthen the bacterial classification existence adaptive capacity of environment to external world, the method for tempering bacterial classification can be on the basis of culture test tube bacterial classification temperature, rose 1 ℃ or descend 1 ℃ through 12 hours, but the highlyest be no more than 30 ℃, minimumly be not less than 16 ℃, the time of alternating temperature refining kind is 2~5 days.Cover with after 6~11 days when mycelia, promptly obtain parent, also claim first class inoculum.Can in 1: 50 ratio expand numerous or be put into preserve under 4 ℃ of temperature stand-by.
Embodiment 2: the method for operating of making the 100Kg kernel culture
Take by weighing selected wheat berry of 98Kg or iblet and water and draw wash clean, adding 0.5Kg calcium carbonate soaked 12 hours, after making it fully suction, leaching wheat or iblet puts into water and boils about 15 minutes, make it thoroughly well cooked but not mushy, take out, adding 1.5Kg calcium sulphate stirs to hand and does not bedew, this moment, water content was about 70%, in pack into 15 * 30cm special bacteria bag or the bottle, at 147 kPas, finished sterilization under 128 ℃ of HTHPs through 2.5 hours, nature step-down cooling, when treating that temperature is reduced to below 30 ℃, at aseptic, the inoculation of the nearly two ends dislocation of packed employing inoculation method; With bottled employing deep layer inoculation, it is even to help the mycelia growing way.Inoculation back is at normal temperature and have under 10~20 lux light scattering conditions and cultivate, through 10~12 days mycelia pursefuls or full bottle both about 200 bags of original seeds, also claim second class inoculum, can be used for expanding numerous or cultivation.
Embodiment 3: make 1000 bag cultivating kinds
Planting a bag specification is the long abdomen bag of 15~17 * 55~57cm polypropylene or polyethylene, and every bag needs material 1Kg, needs 1 dried kilogram compound altogether.Preparation cultivated species medium takes by weighing crushing maize rod core powder 680Kg earlier, weedtree wood chip 200Kg, and wheat bran 200Kg, calcium carbonate 1Kg, sugared 1Kg, clear water 1300Kg is stand-by.
Get 400Kg corncob cellulose powder and 100Kg weed tree sawdust and stir, add the 100Kg wheat bran and stir, add 1Kg calcium carbonate again and stir, add 280Kg corncob cellulose powder more respectively, 100Kg weed tree sawdust, 100Kg wheat bran.Get 10Kg water and 1Kg brown sugar is dissolved fully add 100Kg water dilution back again and add and participate in stirring in the compound, remaining water is added gradually in the compound of stirring, make water content be controlled at 50~55%, stir back machinery pack.Pack and move in the normal-pressure sterilization kitchen range in back six hours, under the temperature under normal pressure, more than 96 ℃ through 16~18 hours, keep 100 ℃ and can finish sterilization, go out when being cooled to below 30 ℃ naturally behind the kitchen range, adopt outside of belly multipoint mode positive double eight or three three nine of rows to beat the cave aseptic inoculation.Put the clean bag of 20 * 60cm after the inoculation again; seal the back with " well " font pile and cultivation naturally is being arranged under 10~20 lux light scattering conditions; get final product purseful through 18~20 days mycelia, get three-class strain, claim cultivation strain or large-scale planting to join the bacterium bacterial classification in vain again.
The present invention provides the preferable assembly of three preparation cultivated species medium, embodiment describes the preparation method of one of them in detail, other two prescriptions are similar with it, during operation, amount takes by weighing major ingredient (weed tree sawdust or rape accessory substance) in proportion, earlier from major ingredient, take out 1/3~1/2, after taking by weighing the stirring of auxiliary material (wheat bran or rice bran) and additive (breeze), add remaining major ingredient again and stir.From material-water ratio is to take out part water 1: 1.2~1.3 the water consumption, with sugar and V BDissolving fully respectively stirs together with joining in the compound after the surplus water dilution, makes its water content reach 50-55%.Press aforesaid operations pack, sterilization then, the control astigmatism is cultivated and is made cultivated species.
The cultivation bacterium bacterium bag that is fit to large-scale planting is moved into three-dimensional layer frame fruiting canopy, remove the bagging of 20 * 60cm and put on the shelf.During grand style with an inoculation eye upper quadrant, 9~10 bags of every grillages, bag rise 6cm, the intact back of grand style reaches more than 90% mushroom grid humidity to fruiting grid humidification, and window every day at normal temperatures and changed to fresh air in 30 minutes, urge behind the flower bud promptly having rose-shaped white ginseng to grow in 3~5 days from the inoculation eye.If allow bacterium bag or bacterium rod inoculation eye place when putting on the shelf earlier downwards, when treating behind the fruiting that mushroom grows to 4~5 maturity, the upset of fruiting eye is cultivated up, promptly grew up to special " lotus shape " white outside Neihuang County white ginseng through 3~5 days, this is to utilize growth of bacterium mushroom and the commodity economy shape that illumination is brought up.But dress is once joined 2~3 batches of bacterium bag fruitings in vain, finishes in about 28 days, and every bag of bacterial classification on average can produce white ginseng one hectogram.

Claims (8)

1, the artificial cultivation raising technology of a kind of white ginseng bacterium by the preferred white ginseng bacterial classification in field, obtains wild strain through the tissue isolation purification, employing PDA, VB agar protein culture medium are packed in the test tube, behind autoclave sterilization, insert bacterial strain, cultivate female kind, also claim the one-level kind; The wheat that boils or iblet are placed in bag or the bottle, high-temperature and high-pressure conditions down the inoculation of sterilization back female plant and cultivate original seed, also claim the secondary kind; By the medium of preparation, the inoculation original seed and cultivate three grades of kinds, also claim the extension of cultivated species or scale to produce kind, it is characterized in that: the prescription composition of cultivated species medium is by weight percentage
1), broad-leaved weedtree wood chip 68~80%, wheat bran 17~30%, calcium sulphate 0.5%, calcium carbonate 0.5%, sugar 1%, VB0.01%, or
2), corncob cellulose powder 50~68%, weedtree wood chip 20~28%, wheat bran 10~20%, calcium carbonate 1%, sugar 1%, or
3), rape accessory substance 50~70%, weed tree sawdust 10~20%, rice bran 10~18%, corn flour 8~10%, calcium carbonate 0.5%, superphosphate 0.5%, sugar 1%,
More than batching is 1: 1.2~1.3 mixing by material-water ratio and stirs, reaching at 50~55% o'clock and pack into the assorted bacterium that does in the bag to go out to water content handles the back and inoculates under aseptic condition, be inserted in again in the clean bag and send into culturing room, under the scattered light irradiation of 10-20 lux, cultivate, the mycelia purseful promptly makes cultivated species, in time move in the grid frame and put on the shelf, grand style, the intact back of grand style to grid frame humidification to the humidity that is not less than 90%, normal temperature was windowed 30 minutes following every day, can grow white ginseng from the inoculation eye in 3~5 days.
2, artificial cultivation raising technology as claimed in claim 1, it is characterized in that: the cultivated species medium by the wood chips of the broad-leaved weedtree seeds that do not contain fat ether and aromatic oil or corncob cellulose powder or rape accessory substance as major ingredient, wheat bran or rice bran or corn flour are auxiliary material, add the mineral matter of trace again, V B, sugar forms as additive, wherein each 50% constitutes the broad-leaved weed tree sawdust by wood chip and wood shavings, the rape accessory substance is made of rape shell 30% and rape straw powder 70%.
3, artificial cultivation raising technology as claimed in claim 1, it is characterized in that: insert in the strain culturing one-level kind process, under the scattered light irradiation of 10-20 lux, cultivate, behind the mycelia full packages, mycelia is cooked alternating temperature refining kind of processing, promptly on the basis of cultivation temperature, raised or reduce 1 ℃, but the highlyest be no more than 30 ℃, minimumly be not less than 16 ℃ through 12 hours, through 2-5 days change temperature hardening bacterial classifications, for cultivate the secondary kind use or be put into preserve under 4 ℃ of temperature stand-by.
4, artificial cultivation raising technology as claimed in claim 1 is characterized in that: the mycelia of bacterial classifications at different levels need be cultivated under the scattered light irradiation of 10-20 lux in the process that expands propagating and cultivating.
5, artificial cultivation raising technology as claimed in claim 1, it is characterized in that: when the inoculation mother plants, the dislocation inoculation of the nearly two ends of the employing that in desinfection chamber, is packed in bag, with bottled employing deep layer inoculation, inoculation back is at normal temperature and have under the light scattering condition of 10-20 lux and cultivate, and mycelia covers with bag or full bottle promptly gets the secondary kind.
6, artificial cultivation raising technology as claimed in claim 1 is characterized in that: the inoculation of cultivated species adopts nine of positive double eight or three three rows' outside of belly multiple spots to beat the cave inoculation method.
7, artificial cultivation raising technology as claimed in claim 1 is characterized in that: the bag cultivating kind that covers with mycelia moves into three-dimensional tier rack type row mushroom grid frame.
8, artificial cultivation raising technology as claimed in claim 1 is characterized in that: inoculate the downward back of eye upper quadrant or elder generation upper quadrant to the bacterial classification moulding with bacterium bag or bacterium rod during grand style.
CNB200410021821XA 2004-02-11 2004-02-11 Schizophyllum commune artificial cultivation and breeding technology Expired - Fee Related CN100399876C (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101803535A (en) * 2010-05-08 2010-08-18 镇江市食用菌研究所 Method for cultivating edible fungi by rape pod shells
CN102090266A (en) * 2010-12-05 2011-06-15 余家贵 High-efficient cultivation method of schizophyllum commune
CN102523935A (en) * 2012-02-23 2012-07-04 蔡家顺 Schizphylhls commne Fr. cultivation method capable of increasing yield exponentially
CN105110934A (en) * 2015-08-27 2015-12-02 马鞍山市安康菌业有限公司 High-efficiency culture medium capable of enhancing biological efficiency of Schizophyllum commune and preparation method thereof
CN105567571A (en) * 2015-12-23 2016-05-11 文山学院 Method for isolating strains of air-dried fruiting bodies of schizophyllum commune
CN105875199A (en) * 2016-05-12 2016-08-24 庞开云 High-and-stable-yield cultivation method for Schizophyllumcommuneh
CN106034734A (en) * 2016-05-31 2016-10-26 博白县那林镇菌兴家庭农场 Cultivation method for increasing yield of auricularia polytricha
CN106754409A (en) * 2016-12-08 2017-05-31 段必儒 White ginseng bacterium efficiently educates plant method
CN107434737A (en) * 2016-05-27 2017-12-05 桂林洁宇环保科技有限责任公司 A kind of new white ginseng bacteria cultivation material
CN107434596A (en) * 2016-05-26 2017-12-05 罗旭 The white ginseng bacterium high-yield culturing material that a kind of corncob pueraria root residue is configured to
CN107746307A (en) * 2017-11-20 2018-03-02 昆明海冠食用菌开发有限公司 Plateau white ginseng bacterium culture medium
CN110506806A (en) * 2019-09-29 2019-11-29 横县南方茶厂 A kind of processing method of six fort tea of white ginseng bacterium jasmine
CN110506808A (en) * 2019-09-29 2019-11-29 横县南方茶厂 A kind of processing method of white ginseng bacterium jasmine white tea
CN111543250A (en) * 2020-06-12 2020-08-18 云南省农业科学院茶叶研究所 Method for cultivating white ginseng fungus by using crude and old tea leaves

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CN1160449C (en) * 2000-01-18 2004-08-04 罗星野 Artificially cultured schizophyllum sporophores and its culture process

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101803535A (en) * 2010-05-08 2010-08-18 镇江市食用菌研究所 Method for cultivating edible fungi by rape pod shells
CN102090266A (en) * 2010-12-05 2011-06-15 余家贵 High-efficient cultivation method of schizophyllum commune
CN102090266B (en) * 2010-12-05 2012-10-31 余家贵 High-efficient cultivation method of schizophyllum commune
CN102523935A (en) * 2012-02-23 2012-07-04 蔡家顺 Schizphylhls commne Fr. cultivation method capable of increasing yield exponentially
CN105110934A (en) * 2015-08-27 2015-12-02 马鞍山市安康菌业有限公司 High-efficiency culture medium capable of enhancing biological efficiency of Schizophyllum commune and preparation method thereof
CN105567571A (en) * 2015-12-23 2016-05-11 文山学院 Method for isolating strains of air-dried fruiting bodies of schizophyllum commune
CN105875199A (en) * 2016-05-12 2016-08-24 庞开云 High-and-stable-yield cultivation method for Schizophyllumcommuneh
CN107434596A (en) * 2016-05-26 2017-12-05 罗旭 The white ginseng bacterium high-yield culturing material that a kind of corncob pueraria root residue is configured to
CN107434737A (en) * 2016-05-27 2017-12-05 桂林洁宇环保科技有限责任公司 A kind of new white ginseng bacteria cultivation material
CN106034734B (en) * 2016-05-31 2019-06-21 谢英格 A kind of cultural method improving Uricularia polytricha yield
CN106034734A (en) * 2016-05-31 2016-10-26 博白县那林镇菌兴家庭农场 Cultivation method for increasing yield of auricularia polytricha
CN106754409A (en) * 2016-12-08 2017-05-31 段必儒 White ginseng bacterium efficiently educates plant method
CN106754409B (en) * 2016-12-08 2020-01-10 段必儒 Method for cultivating and planting holothuria leucorrhoeae
CN107746307A (en) * 2017-11-20 2018-03-02 昆明海冠食用菌开发有限公司 Plateau white ginseng bacterium culture medium
CN110506806A (en) * 2019-09-29 2019-11-29 横县南方茶厂 A kind of processing method of six fort tea of white ginseng bacterium jasmine
CN110506808A (en) * 2019-09-29 2019-11-29 横县南方茶厂 A kind of processing method of white ginseng bacterium jasmine white tea
CN111543250A (en) * 2020-06-12 2020-08-18 云南省农业科学院茶叶研究所 Method for cultivating white ginseng fungus by using crude and old tea leaves

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