CN105110961A - Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same - Google Patents
Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same Download PDFInfo
- Publication number
- CN105110961A CN105110961A CN201510573903.3A CN201510573903A CN105110961A CN 105110961 A CN105110961 A CN 105110961A CN 201510573903 A CN201510573903 A CN 201510573903A CN 105110961 A CN105110961 A CN 105110961A
- Authority
- CN
- China
- Prior art keywords
- liquid
- mushroom
- beans
- dregs
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 81
- 238000000855 fermentation Methods 0.000 title claims abstract description 31
- 230000004151 fermentation Effects 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title abstract description 27
- 240000000599 Lentinula edodes Species 0.000 title abstract description 15
- 239000001963 growth medium Substances 0.000 title abstract description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 32
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 27
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 26
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 26
- 239000008103 glucose Substances 0.000 claims abstract description 25
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 21
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 21
- 238000005516 engineering process Methods 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 235000015099 wheat brans Nutrition 0.000 claims description 23
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 19
- 239000011591 potassium Substances 0.000 claims description 19
- 229910052700 potassium Inorganic materials 0.000 claims description 19
- 229910019142 PO4 Inorganic materials 0.000 claims description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 18
- 239000010452 phosphate Substances 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 17
- 244000068988 Glycine max Species 0.000 claims description 13
- 235000010469 Glycine max Nutrition 0.000 claims description 13
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 9
- 230000002045 lasting effect Effects 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000002386 leaching Methods 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000007858 starting material Substances 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 abstract 1
- 239000001768 carboxy methyl cellulose Substances 0.000 abstract 1
- 235000001727 glucose Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 abstract 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 13
- 108010059892 Cellulase Proteins 0.000 description 12
- 229940106157 cellulase Drugs 0.000 description 12
- 230000006698 induction Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 8
- 235000001715 Lentinula edodes Nutrition 0.000 description 7
- 238000009835 boiling Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 229910001220 stainless steel Inorganic materials 0.000 description 7
- 239000010935 stainless steel Substances 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 239000006260 foam Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000002023 wood Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- -1 poly-hydroxy aldehyde Chemical class 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 241001677738 Aleuron Species 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 208000027219 Deficiency disease Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 229920001491 Lentinan Polymers 0.000 description 1
- 241000222418 Lentinus Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 208000008167 Magnesium Deficiency Diseases 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- YUGCAAVRZWBXEQ-UHFFFAOYSA-N Precholecalciferol Natural products C=1CCC2(C)C(C(C)CCCC(C)C)CCC2C=1C=CC1=C(C)CCC(O)C1 YUGCAAVRZWBXEQ-UHFFFAOYSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 229910052787 antimony Inorganic materials 0.000 description 1
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004939 coking Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229940115286 lentinan Drugs 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 235000004764 magnesium deficiency Nutrition 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 125000003071 maltose group Chemical group 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 125000005341 metaphosphate group Chemical group 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a liquid fermentation culture medium for shiitake mushrooms. The liquid fermentation culture medium is composed of main raw materials including bran, bean pulp, glucose, monopotassium phosphate, magnesium sulfate and sodium carboxymethylcellulose. The invention further provides a method for producing the shiitake mushrooms through the liquid fermentation culture medium for the shiitake mushrooms with the liquid strain technology. By means of the liquid fermentation culture medium and the method, a traditional method for producing shiitake mushroom strains is changed, the liquid strain technology is adopted in shiitake mushroom strain production, the production efficiency is improved, and the production cost is greatly saved; in addition, the production method is simple in technology, the produced strains are high in use rate, and the pollution rate is low.
Description
Technical field
The invention belongs to Edible Fungi field, particularly relate to a kind of mushroom liquid fermentation medium and utilize liquid spawn technology to produce the method for mushroom.
Background technology
Mushroom, another name mushroom, fragrant bacterium, be the sporophore of Pleurotaceae plant mushroom, nutritive value is very high.Mushroom is second-biggest-in-the-world edible mushrooms, one of Ye Shi China special product, at the title have " mountain delicacy " among the people.
Lentinus edodes-protein content is up to 18.6%, lipid content is low to moderate 4.8%, carbohydrate content is 71%, crude fiber content is 9.6%, 8 seed amino acids, multivitamin, mineral element etc. containing needed by human, particularly rich vitamin B group, iron, potassium, provitamin D (changing into vitamins D after Exposure to Sunlight).
Mushroom has multiple biological activity and pharmacological action.Mushroom taste is sweet, and property is put down, and cure mainly appetite stimulator, weak breath is weak, is high protein, low-fat nutritive health-care food.In mushroom, Quantitative Determination of Ergosterol is very high, effective to preventing and treating rickets; Lentinan (β ~ 1,3 dextran) can strengthen cell immunocompetent, thus the growth of anticancer; Mushroom contains more than 40 kind of enzyme of six large enzymes, can correct human chitinase deficiency disease; Fatty institute fatty acids in mushroom, reduces blood fat to human body useful.
Tradition mushroom production generally adopt solid spawn to carry out inoculation culture, bacterial classification used is the Conventional solid such as Saw-dust, tree fungus bacterial classification mainly, and the method production cycle is long, generally need 55-60 days, technique is cumbersome, production method efficiency is low; Bacterial classification production process needs larger space, need throw a large amount of human and material resources of people, cost is high; The mycelium culture cycle is long, and cultivate substrate mycelium cell age and differ greatly up and down, when front end mycelia is in and sprouts state, stromal surface mycelia, close to aging, cause cultivar fruiting irregular, can not reach mushroom large-scale production, be difficult to the production requirement meeting people.The substitute is and adopt liquid culture technology to produce, liquid culture has with short production cycle, mycelial growth is fast, and mycelia good dispersity, germination point are many, the short period of time can obtain a large amount of mycelium and meta-bolites, and be suitable for the features such as factorial praluction, its mycelium and fermented liquid can be utilized to produce healthcare products and extracting effective components, is a brand-new route of development Lentnus edodes.
But adopt liquid fermenting to produce in the process of mushroom, it is crucial that whether the formula of liquid nutrient medium used is excellent, whether culture condition is suitable for, and it directly decides the formation of the metabolism and growth product of shiitake mushroom hypha.In prior art, Xu Siwei etc. are by being optimized mushroom liquid bacterial culture condition, the carbon source, nitrogenous source, liquid spawn culture medium, culture condition etc. that are suitable for are screened multifactor, namely determine that the most applicable carbon source is maltose, because there is not obvious coking phenomenon in autoclaving process in contrary glucose, cause practical efficiency lower, and its production cycle need 20-30 days.In above-mentioned document, for the research of the method for liquid nutrient medium or liquid culture mushroom, rest on research experiment of single factor aspect more, also little for the repercussion study in mushroom liquid fermenting process between each factor, fail fundamentally to solve the problem that Lentnus edodes method efficiency is low, cost is high, the mycelium culture cycle is long.
Summary of the invention
Low in order to solve Lentnus edodes method efficiency in prior art, cost is high, the problem that the mycelium culture cycle is long, the invention provides the mushroom liquid bacterial production method that a kind of production efficiency is high, the mycelium culture cycle is short, cost is low.
The present invention is achieved through the following technical solutions:
A kind of mushroom liquid fermentation medium, by weight percentage, comprise following component: wheat bran 2%, dregs of beans 1%, glucose 0.6%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, Xylo-Mucine 0.02%, surplus is water.
Described wheat bran, dregs of beans purity are more than 90%, and described glucose, potassium primary phosphate, magnesium sulfate, Xylo-Mucine are analytical pure.
Wherein, Xylo-Mucine, as cellulase induction element, plays the effect that induction produces cellulase in the medium.Enzyme in microorganism cells can be divided into constitutive enzyme and inducible enzyme, and constitutive enzyme is the enzyme always existed in cell, and its synthesis only controls namely to control by internal cause by genetic material; Inducible enzyme is when having inductor (being generally the substrate of reaction) to exist in the environment, it is exactly inducible enzyme that microbes produces a kind of enzyme because inductor exists, mushroom is as a kind of eukaryotic microorganisms, cellulase is produced under the induction of inducer Xylo-Mucine, the xylogen in wood chip can be decomposed, xylogen is cellulosic one, reaches the effect of the cellulignin made full use of in wood chip.
Present invention also offers a kind of method that liquid spawn technology produces mushroom, comprise the following steps:
Get the raw materials ready: by weight percentage, take wheat bran 2%, dregs of beans 1%, glucose 0.6%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, Xylo-Mucine 0.02%, for subsequent use;
Dosing: add water the starting material taken stirring and dissolving, boil after lasting 20 minutes and filter, filtrate adds water constant volume, adds fresh soya-bean oil 0.07%, obtains liquid for subsequent use;
Sterilizing: when vapor pressure 0.13-0.15Mpa, temperature 121 DEG C, fermentation ullage is disappeared 2 hours; Fermentor tank is loaded on, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 condition by preparing the liquid tank obtained in above-mentioned dosing step;
The preparation of liquid spawn: treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically inoculate, injects mushroom shaking flask liquid mother and plants 1000mL, inoculum size with volume percent for 2%; Fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 7.0, ferments 6 days, has both obtained mushroom plant and has produced liquid spawn;
Cultivate: to mushroom charge bar through 121 DEG C, vapor pressure 0.13-0.15Mpa sterilizing 2 hours, be cooled to less than 28 DEG C, liquid spawn is utilized to inoculate, inoculum size 20mL/ rod, after through mycelium culture, management of producing mushroom, the operation such as to gather, namely obtain the mushroom utilizing liquid spawn technology to produce.
In above-mentioned cultural method, mushroom charge bar sterilising temp 121 DEG C in preferred culturing process, vapor pressure 0.13-0.15Mpa, is cooled to less than 28 DEG C, inoculum size with volume percent for 2%.
A kind of mushroom liquid bacterial production method of the present invention, preferably comprises the following steps:
One, according to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filter after boiling lasting 20min, add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.
Two, fermentor tank sky under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions disappears 2 hours; The tinning of liquid fermentation tank substratum is prepared, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions by above-mentioned.Treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically inoculate, inoculum size is 2% (v/v).
Three, fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 7.0, ferments 6 days, obtains mushroom plant and produces liquid spawn.
Four, to lentinus edodes strain stick through sterilizing, cooling, through mycelium culture, management of producing mushroom, the operation such as to gather after utilizing liquid spawn to inoculate, namely complete the application that liquid spawn produces lentinus edodes strain stick.
Wheat skin of the present invention and wheat bran are the kernel seed coats sifted out after flour got by wheat processing mill.Wheat skin main component comprises: fiber, aleuron, some minerals and vitamins.
Dregs of beans of the present invention is a kind of byproduct obtained after soybean extracting bean oil.Different according to the method extracted, a leaching dregs of beans and two leaching dregs of beans two kinds can be divided into.Be wherein a leaching dregs of beans with the byproduct after restricted-access media soya-bean oil, and first get oil with squeezing, then get the byproduct of gained after oil through lixiviate and be called two leaching dregs of beans.In the whole course of processing, very important to the control of temperature, the too high meeting of temperature has influence on protein content, thus is directly connected to quality and the use of dregs of beans; The too low water content that can increase dregs of beans of temperature, water content height then can affect the quality of dregs of beans in the shelf lives.The production technique of one leaching dregs of beans is comparatively advanced, and protein content is high, is the principal item circulated in the current spot market of China.
Glucose of the present invention is also called corn sugar, corn sugar, referred to as glucose.Be the widest and of paramount importance a kind of monose of distributed in nature, it is a kind of poly-hydroxy aldehyde.Pure glucose is clear crystal, and pleasantly sweet but sweet taste is not as sucrose (common people cannot taste sweet taste), soluble in water, is slightly soluble in ethanol, is insoluble to ether.Aqueous solution optically-active to the right, therefore belongs to " dextrose ".Glucose has critical role in field of biology, is the energy derive of viable cell and metabolic intermediate product, namely biological main energy supply material.Plant produces glucose by photosynthesis.In candy making industry and field of medicaments extensive application.
Potassium primary phosphate of the present invention (chemical formula: KH
2pO
4) sealing preservation, stable in air, 400 DEG C time, losing water, become metaphosphate, for preparing damping fluid, measuring arsenic, antimony, phosphorus, aluminium and iron, preparation phosphorus reference liquid, preparation substratum, inorganic phosphorus, alkaline phosphatase activity in mensuration serum.Each province is applicable to all kinds of Typical crops for potassium primary phosphate has carried out a lot of related application experiments by the institutes of agricultural sciences of locality, clay fertilizer Zhan Deng expert mechanism, prove that potassium primary phosphate has remarkable increasing both production and income, the amount of changing optimizes quality by the actual application effect of various places to all types of crop, resistant to lodging, disease and insect resistance, many excellent effects such as control early ageing.Potassium sulfate and sodium sulfate outward appearance are turned white, and potassium dihydrogen phosphate crystal is transparent, therefore can from simply identifying in appearance.Potassium primary phosphate is also generally used for Edible Fungi and does trace element interpolation.
Magnesium sulfate of the present invention belongs to the high magnesium sulfate of purity, at agricultural and gardening, magnesium sulfate is used to the soil (magnesium is the fundamental of a chlorophyll molecule) improveing magnesium deficiency, modal for potted plant, or containing magnesium crop, as potato, rose, tomato, capsicum and hemp.The advantage using magnesium sulfate exceedes the high resolution that other magnesium sulfate magnesia soil improvers (as white clouds matter lime) are them.Magnesium sulfate is also generally used for Edible Fungi and does trace element interpolation.
CMC-Na of the present invention (Xylo-Mucine): be the derivatived cellulose that natural cellulose obtains after chemical modification, it is cellulosic carboxymethylether compound, Xylo-Mucine, as cellulase induction element, plays the effect that induction produces cellulase in the medium.
Beneficial effect of the present invention is mainly reflected in the following aspects:
(1) overcome glucose in prior art and as the technological deficiency of the carbon source of optimum, in the selection of liquid medium within, even if using glucose as carbon source, still cannot can obtain the liquid nutrient medium that formula is excellent;
(2) have employed Xylo-Mucine in liquid nutrient medium, adding of this raw material makes mushroom as a kind of eukaryotic microorganisms, cellulase is produced under the induction of inducer Xylo-Mucine, the xylogen in wood chip can be decomposed, xylogen is cellulosic one, reaches the effect of the cellulignin made full use of in wood chip.
(3) with short production cycle: mushroom solid spawn is cultivated needs 55-60 days, and the mushroom liquid bacterial production cycle of the present invention is 14-15 days, and fermentation time 6 days, the cycle is short, and speed is fast.
(4) production process realizes automatically controlling: achieve on-line automatic dissolved oxygen at fermentation procedure and detect fermentor tank dissolved oxygen, sterilizing, shaking flask, cultivation all realize automatic control.
(5) cost-saving: liquid spawn production cost is 1/3rd of solid spawn production cost, and output is high, and raw material is cheap.
(6) cell age is consistent: liquid spawn mycelia is energetic, and mycelial growth rate is comparatively consistent, buddings and fruiting time consistency, is convenient to manage, gathers, processes.
(7) soya-bean oil is added in culturing process of the present invention, described soya-bean oil source easily, price is low, belong to simple defoamer, in liquid spawn, make foam local surface tension reduce cause bubble burst, be natural defoamer, the origin of this kind of mechanism is sprinkling upon on foam by vegetables oil, when it dissolves in foam solution, the surface tension at this place significantly can be reduced.Because these materials are generally less to the solubleness of water, capillary reduction is only limitted to the local of foam, and the surface tension around foam has almost no change, and the part that surface tension reduces is drawn to surrounding consumingly, extended, and finally breaks.
(8) compared with solid spawn cultural method, strain cultivation pollution rate of the present invention is low to moderate 3 ‰, and solid spawn cultivates pollution rate up to 10%; The liquid spawn bacteria phase of the present invention is as short as 40 days, and solid spawn cultivation pollution rate reaches 60 days; The strain cultivation time of the present invention only needs 96 hours, and solid spawn cultivation reaches 55-60 days; During strain cultivation of the present invention, sterilising temp only needs 121 DEG C, 40-50 minute, greatly saves energy consumption, and solid spawn cultivate time sterilising temp up to 125 DEG C, keep 3.5-4 hour, during energy charge; Liquid spawn of the present invention inoculates 10,000 rods every day only needs 2 people, and solid spawn inoculation needs 8 people, liquid-spawn inoculation method can be saved artificial up to 75%.
Embodiment
Wheat bran involved in following examples, dregs of beans purity are more than 90%, and described glucose, potassium primary phosphate, magnesium sulfate, Xylo-Mucine are analytical pure.
Embodiment 1
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.Fermentor tank sky under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions disappears 2 hours; The tinning of liquid fermentation tank substratum is prepared, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions by above-mentioned.Treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically inoculate, inoculum size is 2% (v/v).Fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 7.0, ferments 6 days, has both obtained mushroom plant and has produced liquid spawn.To lentinus edodes strain stick through sterilizing, cooling, through mycelium culture, management of producing mushroom, the operation such as to gather after utilizing liquid spawn to inoculate, namely complete the application that liquid spawn produces lentinus edodes strain stick.
Embodiment 2
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.Fermentor tank sky under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions disappears 2 hours; The tinning of liquid fermentation tank substratum is prepared, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions by above-mentioned.Treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically inoculate, inject fragrant mushroom Oscillating bottle liquid mother and plant 1000mL, inoculum size is 2% (v/v).Fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 7.0, ferments 6 days, has both obtained mushroom plant and has produced liquid spawn.To mushroom charge bar through 121 DEG C, vapor pressure 0.13-0.15Mpa sterilizing 2 hours, be cooled to less than 28 DEG C, liquid spawn is utilized to inoculate, inoculum size 20mL/ rod, after through mycelium culture, management of producing mushroom, the operation such as to gather, namely complete the application that liquid spawn produces lentinus edodes strain stick.
Comparative example 1
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, does not add Xylo-Mucine, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.Sterilizing, cultural method are in the same manner as in Example 1.
Comparative example 2
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 4000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, do not add fresh soya-bean oil, for subsequent use.Sterilizing, cultural method are in the same manner as in Example 1.
Comparative example 3
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, glucose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.Fermentor tank sky under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions disappears 2 hours; The tinning of liquid fermentation tank substratum is prepared, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions by above-mentioned.Treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically inoculate, inoculum size is 2% (v/v).Fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 5.0, ferments 6 days, has both obtained mushroom plant and has produced liquid spawn.To lentinus edodes strain stick through sterilizing, cooling, through mycelium culture, management of producing mushroom, the operation such as to gather after utilizing liquid spawn to inoculate, namely complete the application that liquid spawn produces lentinus edodes strain stick.
Comparative example 4
According to wheat bran formula, take by 500L standard fermentor: wheat bran 10000g, dregs of beans 5000g, maltose 3000g, potassium primary phosphate 500g, magnesium sulfate 250g, CMC-Na (cellulase induction element) 100g, put into 200L stainless steel decoction pot, add water 100L stirring and dissolving, filters after boiling lasting 20min, and add water filtrate constant volume 500L, and add fresh soya-bean oil 350g, for subsequent use.Sterilizing, cultural method are in the same manner as in Example 1.
By the hypha biomass in embodiment 1 and comparative example 1-4 production process for prepare, every test group repeats 3 times and averages, and the impact on mushroom mycelium biomass under research different condition, the results are shown in Table 1.
Mushroom mycelium biomass under table 1. different condition
Note: hypha biomass unit is g/100ml
As seen from the results in Table 1, comparative example 1 only eliminates Xylo-Mucine, and in its production process, mushroom mycelium biomass significantly decreases, and the introducing of Xylo-Mucine is described, can significantly improve productive rate and effect that liquid spawn method produces mushroom.Comparative example 2, comparative example 4 change the consumption of glucose and the kind of carbon source respectively, and after changing, mushroom mycelium biomass significantly decreases, and the introducing of glucose is described, can significantly improve productive rate and effect that liquid spawn method produces mushroom.Comparative example 3 changes initial pH value in culturing process, and mushroom mycelium biomass significantly decreases, and the rising of pH value is described, can significantly improve productive rate and effect that liquid spawn method produces mushroom.
Above-mentioned experiment absolutely proves, compared to prior art, the nutritive ingredient contained by liquid nutrient medium of the present invention more can meet the demand of mushroom mycelium to nutrition, and after wheat bran pyroprocessing, soluble protein content is higher, be rich in various VITAMIN, suitable mycelial growth simultaneously.Be that carbon source mycelial growth is fast with glucose, biomass is high, and cheap, is the raw material of mushroom liquid nutrient medium first-selection.Coordinate liquid nutrient medium of the present invention, the organic carrier that the bacterial classification that the liquid spawn technology that the present invention adopts is produced then adds in bacterial classification assimilation process. not only mycelium pellet (mycelia) is played to the effect improving the ability that shakes down, extend the bacterial classification life-span, also carried out, without the need to the amplification of cultivating, reducing bacterial classification cost to bacterial classification simultaneously.
Although contriver has done comparatively detailed elaboration to technical scheme of the present invention and has enumerated; be to be understood that; for one, this area those skilled in the art; amendment is made to above-described embodiment or adopts equivalent replacement scheme; this is apparent to those skilled in the art; these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (5)
1. a mushroom liquid fermentation medium, is characterized in that: by weight percentage, comprises following component: wheat bran 2%, dregs of beans 1%, glucose 0.6%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, Xylo-Mucine 0.02%.
2. mushroom liquid fermentation medium according to claim 1, is characterized in that, described wheat bran, dregs of beans purity are more than 90%, and described glucose, potassium primary phosphate, magnesium sulfate, Xylo-Mucine are analytical pure.
3. mushroom liquid fermentation medium according to claim 2, is characterized in that, described dregs of beans is preferably a leaching dregs of beans.
4. liquid spawn technology produces a method for mushroom, it is characterized in that, comprises the following steps:
Get the raw materials ready: by weight percentage, take wheat bran 2%, dregs of beans 1%, glucose 0.6%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, Xylo-Mucine 0.02%, for subsequent use;
Dosing: add water the starting material taken stirring and dissolving, boil after lasting 20 minutes and filter, filtrate adds water constant volume, adds fresh soya-bean oil 0.07%, obtains liquid for subsequent use;
Sterilizing: when vapor pressure 0.13-0.15Mpa, temperature 121 DEG C, fermentation ullage is disappeared 2 hours; Fermentor tank is loaded on, the real 40min that disappears under vapor pressure 0.13-0.15Mpa, temperature 121 DEG C of conditions by preparing the liquid tank obtained in above-mentioned dosing step;
The preparation of liquid spawn: treat that fermentation pot liquid internal temperature is cooled to less than 28 DEG C, aseptically injects fragrant mushroom Oscillating bottle liquid mother and plants 1000mL, inoculum size with volume percent for 2%; Fermentation culture temperature is 23 ± 2 DEG C, and ventilation is 105 ± 5m
3/ h, initial pH value 7.0, ferments 6 days, obtains mushroom plant and produces liquid spawn;
Cultivate: by obtain mushroom liquid bacterial sterilizing, cooling, utilize liquid spawn to inoculate, after through mycelium culture, management of producing mushroom, the operation such as to gather, namely obtain the mushroom utilizing liquid spawn technology to produce.
5. method according to claim 4, is characterized in that, in described culturing process, sterilising temp is 121 DEG C, mushroom charge bar, through 121 DEG C, vapor pressure 0.13-0.15Mpa sterilizing 2 hours, is cooled to less than 28 DEG C, liquid spawn is utilized to inoculate, inoculum size 20mL/ rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510573903.3A CN105110961B (en) | 2015-09-10 | 2015-09-10 | A kind of mushroom liquid fermentation medium and its method for producing mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510573903.3A CN105110961B (en) | 2015-09-10 | 2015-09-10 | A kind of mushroom liquid fermentation medium and its method for producing mushroom |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105110961A true CN105110961A (en) | 2015-12-02 |
| CN105110961B CN105110961B (en) | 2018-07-31 |
Family
ID=54659126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510573903.3A Active CN105110961B (en) | 2015-09-10 | 2015-09-10 | A kind of mushroom liquid fermentation medium and its method for producing mushroom |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105110961B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106008035A (en) * | 2016-07-28 | 2016-10-12 | 柳州创宇科技有限公司 | Lentinus edodes culture medium and lentinus edodes cultivation method thereof |
| CN107548890A (en) * | 2017-10-24 | 2018-01-09 | 翔天农业开发集团股份有限公司 | The liquid cultivating method of mushroom |
| CN107653192A (en) * | 2017-10-25 | 2018-02-02 | 西华师范大学 | A kind of culture medium prescription and method of the preservation of asparagus parent species |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
| CN108739067A (en) * | 2018-06-06 | 2018-11-06 | 湖南科技大学 | A kind of method and application shortened oyster mushroom liquid spawn and be inoculated with bacterium bag bacteria developing period |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
| CN112136602A (en) * | 2020-09-19 | 2020-12-29 | 灌南云农食用菌研究所(有限合伙) | Liquid cultivation culture solution and liquid cultivation production process for velvet antler mushrooms |
| CN112219640A (en) * | 2020-10-16 | 2021-01-15 | 武汉迪奥药业有限公司 | Continuous preparation culture medium for shiitake mushroom fermentation liquid seeds and preparation method |
| CN116024105A (en) * | 2023-02-15 | 2023-04-28 | 上海市农业科学院 | Lentinus edodes liquid strain fermentation medium, its preparation method and fermentation culture method |
| CN117678474A (en) * | 2023-12-06 | 2024-03-12 | 贵州省康群缘生物科技有限公司 | A culture medium for the preservation of Lentinus edodes strains and its preparation method and application |
| CN119234938A (en) * | 2024-10-25 | 2025-01-03 | 云南农业大学 | A kind of beef cattle feed additive and preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041808A (en) * | 2007-02-28 | 2007-09-26 | 上海浦东天厨菇业有限公司 | Golden mushroom factory-production of liquid bacterial culture medium and preparation method thereof |
| CN102850127A (en) * | 2012-09-10 | 2013-01-02 | 阜阳市德益农业科技有限公司 | Pleurotus eryngii liquid strain culture medium |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
-
2015
- 2015-09-10 CN CN201510573903.3A patent/CN105110961B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041808A (en) * | 2007-02-28 | 2007-09-26 | 上海浦东天厨菇业有限公司 | Golden mushroom factory-production of liquid bacterial culture medium and preparation method thereof |
| CN102850127A (en) * | 2012-09-10 | 2013-01-02 | 阜阳市德益农业科技有限公司 | Pleurotus eryngii liquid strain culture medium |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 沈业寿等: "诱导剂CMC-Na对食用菌生长的影响", 《食用菌》 * |
| 王谦等: "香菇的深层发酵工艺应用研究", 《河北大学学报(自然科学版)》 * |
| 范贵增等: "液体发酵生产食用菌菌丝体条件的研究", 《食品与发酵工业》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106008035A (en) * | 2016-07-28 | 2016-10-12 | 柳州创宇科技有限公司 | Lentinus edodes culture medium and lentinus edodes cultivation method thereof |
| CN107548890A (en) * | 2017-10-24 | 2018-01-09 | 翔天农业开发集团股份有限公司 | The liquid cultivating method of mushroom |
| CN107653192A (en) * | 2017-10-25 | 2018-02-02 | 西华师范大学 | A kind of culture medium prescription and method of the preservation of asparagus parent species |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
| CN108739067A (en) * | 2018-06-06 | 2018-11-06 | 湖南科技大学 | A kind of method and application shortened oyster mushroom liquid spawn and be inoculated with bacterium bag bacteria developing period |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
| CN112136602A (en) * | 2020-09-19 | 2020-12-29 | 灌南云农食用菌研究所(有限合伙) | Liquid cultivation culture solution and liquid cultivation production process for velvet antler mushrooms |
| CN112219640A (en) * | 2020-10-16 | 2021-01-15 | 武汉迪奥药业有限公司 | Continuous preparation culture medium for shiitake mushroom fermentation liquid seeds and preparation method |
| CN116024105A (en) * | 2023-02-15 | 2023-04-28 | 上海市农业科学院 | Lentinus edodes liquid strain fermentation medium, its preparation method and fermentation culture method |
| CN117678474A (en) * | 2023-12-06 | 2024-03-12 | 贵州省康群缘生物科技有限公司 | A culture medium for the preservation of Lentinus edodes strains and its preparation method and application |
| CN119234938A (en) * | 2024-10-25 | 2025-01-03 | 云南农业大学 | A kind of beef cattle feed additive and preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105110961B (en) | 2018-07-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105110961B (en) | A kind of mushroom liquid fermentation medium and its method for producing mushroom | |
| CN103333022B (en) | Cultivation material compatibility of agrocybe cylindracea and manufacture method of cultivation material | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN104620856A (en) | Method for cultivating hericium erinaceus by employing ramulus mori | |
| CN103155785A (en) | Establishment method for brown mushroom three-level strain propagation system | |
| CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
| CN106613334A (en) | Nutrient-rich pleurotus eryngii cultivation method | |
| CN104641942A (en) | Method for cultivating oyster mushroom on mulberry twigs | |
| CN105176839A (en) | Culture medium of mushroom liquid strain and method of producing mushroom bacterium stick | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| CN103922851A (en) | Black fungus liquid medium and black fungus cultural method | |
| CN105237248A (en) | Grifola frondosa production culture medium and application thereof | |
| CN102726210B (en) | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus | |
| CN100399876C (en) | Schizophyllum commune artificial cultivation and breeding technology | |
| CN105948831B (en) | A kind of method of using brewer's grains to produce bio-control fertilizer | |
| CN107360858A (en) | A kind of breeding method of mushroom edible mushroom | |
| CN104885787A (en) | Planting method for cordyceps militaris sporocarp high in active matter content | |
| CN103004453A (en) | Manufacturing method of edible fungi cultivar and culture medium manufacturing raw materials for edible fungi cultivar | |
| CN106718021A (en) | A kind of yield Volvaria volvacea cultivation method high | |
| CN103005437B (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN109526548A (en) | A kind of black fungus bag stuff cultivation method based on cotton seed hulls | |
| CN108718915A (en) | Improve the culture medium and cultural method of pleurotus edible fungus yield | |
| CN108293612A (en) | The culture medium of cultivating agaricus bisporus and the method for cultivating White mushroom using it | |
| CN106977245A (en) | A kind of compost of selenium enriched oyster mushroom and the cultural method of selenium enriched oyster mushroom | |
| CN105110852A (en) | Liquid strain culture medium for shiitake mushrooms and method for culturing shiitake mushrooms in three-dimensional bag hanging mode |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |