CN102090266B

CN102090266B - High-efficient cultivation method of schizophyllum commune

Info

Publication number: CN102090266B
Application number: CN2010105786192A
Authority: CN
Inventors: 余家贵
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-12-05
Filing date: 2010-12-05
Publication date: 2012-10-31
Anticipated expiration: 2030-12-05
Also published as: CN102090266A

Abstract

The invention discloses a high-efficient cultivation method of schizophyllum commune. The method comprises the following steps of: inoculating a cultivar cultivated by domesticating wild schizophyllum commune into a cultivar culture medium which is prepared by components such as barley seeds or buckwheat seeds, broad leaf saw dust and the like and is subjected to high temperature and high pressure treatment; directly sheltering, shelving and arranging; exhausting air through a pricked hole; unsealing; cutting, and the like. Mushroom fruiting is managed at a proper temperature and high humidity; the grown schizophyllum commune has high quality; and the method is high in economic benefit, is easy to operate and practicable, and is an ideal schizophyllum commune manual planting technology.

Description

The efficient cultivation of white ginseng bacterium

Technical field

The invention belongs to artificial cultivation edible mushroom technology, the efficient cultivation of especially a kind of white ginseng bacterium.

Background technology

Join Pseudomonas in vain in Basidiomycotina, Hymenomycetes, Agaricales, schizophyllum commune section, Pleurotus has another name called schizophyllum commune, and popular name eight load bavins are the wider wild edible fungus of a kind of distribution.Its adaptation temperature spoke broad, mycelia can grow in 6-30 ℃ of scope, and low energy is 8 ℃ of growth fruit bodys; The fruit body optimum growth temp is 14-28 ℃, and 30 ℃ of limit high temperature are had relatively high expectations to moisture content; Fruit body optimum growh air humidity is 80-90%, is lower than dehydration death in 55% short time.Because white ginseng bacterium is rich in multiple organic matter, contains 17 kinds in needed by human body 18 seed amino acids, contains 7 kinds of 8 kinds of trace elements of needed by human body; And contain antitumor, antibiotic synergist, many of schizophyllum communes, the delicious delicate fragrance of edible taste; Often ediblely clear liver and improve vision, stomach-invigorating intestine-moisturizing; The function of nourishing and fit keeping function all has remarkable result to infantile night sweat, hemorrhoid disease, constipation, neurasthenia, dizzy tinnitus, dyssomnia, the ability enhancing human immune; Can assist tumor treatment, be a kind of of great value food medicine dual purpose product.But the pure wild little flavor of a white ginseng bacterium shape is old; Output is limited, can not meet the need of market, and people have also obtained some progress aspect the white ginseng of the cultivation bacterium taming and dociling to educate; Tame white ginseng bacterium quality, taste, output all are better than wild bacterium; Like the patent No. is that artificial cultivation raising technology that ZL200410021821.X joins bacterium in vain is wherein a kind of, and through the wild white ginseng bacterium of seed selection domestication, and successful breeding cultivation has been produced quality, output and is superior to wild white living bacterium greatly.But should the cover technology still have deficiency: the medium batching component of cultivated species is too much, reach the 5-7 kind, and the nutriment that can provide is not enough; The white ginseng bacterium of planting out except that a first planting shape, output better; Several batches of backs are obvious weak, are difficult to reach high yield, stable yields, and the economy price ratio is not high; And, seem too cumbersome in the operation.

Summary of the invention

For remedying above-mentioned deficiency, the invention reside in few, the comprehensive nutrition of a cover component, the cultivated species medium that content is high are provided, bigger to ensure a white ginseng bacterium shape of producing; Outward appearance is more attractive in appearance, and stable and high yields can be adopted many batches; Economic benefit is high, and the simple white ginseng bacteria cultivation method of operating technology.

The present invention accomplishes like this: preferred fresh and alive wild white ginseng bacterial strain is inoculated in potato agar albumen test tube medium with separate tissue or spore separation method; Cultivating purifies obtains female the kind; Mother planted cultivate in the buckwheat grain medium be inoculated in vial purify original seed; After again the culture medium for cultivating bag of handling well being prepared in the original seed access, directly move into the plastic tunnel of thermophilic high humidity, observe the management fruiting.

The technical characterictic of this method is that the cultivated species medium has three kinds of prescriptions, by the percentage by weight of preparing burden is:

1. wheat berry 50%, broad-leaved weedtree sawdust 49%, gesso 1%;

2. barley corn 60%, broad-leaved weedtree sawdust 30%, wheat bran 9%, gesso 1%;

3. the buckwheat grain 60%, broad-leaved weedtree sawdust 35%, wheat bran 4%, gesso 1%;

Batching is mixed all, add water by material-water ratio 1: 1.3-1.5 and stir, making water content is 55%-60%, pinches not drip with hand and is advisable; Divide the bag of packing into, tighten mouth and insert wet sterilization pot pressurization 0.147MPa, temperature control keeps sterilization in 90 minutes, cooling naturally for 128 ℃; Pressure is reduced to the taking-up sack that boiled at 0 o'clock, when temperature is reduced to below 25 ℃, inserts the original seed make with sterile working, with direct put on the shelf 14-28 ℃ of the grand style temperature control of an immigration booth in non-drying adhesive tape sealing label inoculation eye back; Damping 80-90% carries out management of producing mushroom, can adopt the first planting and join bacterium in vain in 15-18 days; Manage and protect 10 days with method and can adopt regrowth hair, receive the 3-4 stubble continuously, eliminate the bacterium tube after 50 days.

The inoculation of above-mentioned cultivated species takes to keep flat culture bag with inoculation bastinade cave one row's four eyed, and every is sealed with non-drying adhesive tape after putting into 20-25 grain original seed buckwheat grain.

Above-mentioned cultivated species inserts back 3 days; Cover with inoculation when mycelia and at the moment puncture the exhaust of sealing compound cloth with 0.3cm diameter iron nail; Threw off in 8-9 days and seal adhesive plaster, and cut and scratch each 1 of sack 1.5cm osculum in mycelia haloing limit, both sides up and down, form 12 fruiting eyes of three rows at inoculation eye.

Above-mentioned mother's kind, pedigree seed culture medium adopted pressure 0.147MPa, temperature 128-130 ℃ of steam pressure retorts sterilization 40-60 minute.

The white ginseng bacterium that cultivates by this law is piece big, the profile endoplasm is all excellent, and the more former method of output improves more than 1 times, and insert behind the original seed and can need not pile bavin and cultivate directly into the canopy grand style of putting on the shelf, a whole set of production routine and easy to operation, economic benefit is high.

Provide embodiments of the invention below:

Embodiment

With picking up from open-air fresh and alive white ginseng bacterium after simple the processing; Sterile working in inoculating hood inserts the test tube potato agar protein culture medium inclined-plane for preparing to white ginseng bacterial strain tissue, obtains female the kind through cultivating to purify; Again mother is planted and insert in the vial buckwheat grain medium for preparing; It is subsequent use that the cultivation purification obtains original seed, and the vial of 1 500ml is installed in 0.43 jin of dried buckwheat grain, and the original seed for preparing can supply the inoculation of 40-50 bag cultivating kind.Taking by weighing 60kg does not have barley corn that the worm that goes mouldy stays and puts into 0.2% limewash and soak that to pick up the back flushing with clean water in 40 hours clean, takes by weighing 30kg broad-leaved weedtree sawdust and 9kg wheat bran again, the 1kg gesso; Add water 130kg after the material loading mixing is mixed thoroughly and fully stir, pinch not drip with hand and be advisable, water content is 60%; Divide to put in the polyethylene bacterium bag of 15 * 55cm, every bag of 1kg puts into the special-purpose wet sterilization pot 0.147MPa that pressurizes after sack tightened; 128 ℃ of temperature controls kept 90 minutes, naturally cooling; Pressure is 0 o'clock taking-up pocket that boils, and when temperature is reduced to below 25 ℃, just can obtain 100 cultivation bags.Mother's kind of above-mentioned preparation, pedigree seed culture medium also will same high pressure-temperature sterilization treatment just can use after 40-60 minute in the wet sterilization pot equally.In transfer room, cultivation bag is kept flat with loop-carrier four inoculation eyes of horizontal bundle one row along bag, put into 20-25 grain original seed buckwheat grain for every, seal, can obtain 100 bag cultivating bacterial classifications with non-drying adhesive tape; Directly move in the booth grand style and put on the shelf, under 14-28 ℃ of temperature, inoculate that back 3 days mycelia are covered with whole inoculation eye and an inside layer depth is gone into; This moment, the nail with diameter 0.3cm punctured the venting of sealing compound cloth, covered with sack half after 5-6 days mycelia, and had the sudden and violent property mushroom of part to emerge from the adhesive plaster edge; Can throw off adhesive plaster, simultaneously the inoculation eye up and down both sides mycelia haloing edge with pocket knife sack is scratched each 1 of the osculum of 1.5cm, make whole bacterium bag be 12 of horizontal three rows' fruiting mouth; Most of fruiting mouths all grew small mushroom bud after 2-3 days, and make humidity keep 80-90% when continuous spray form water this moment; Can pluck first batch of white ginseng bacterium after 5 days, the same manner management can be adopted second batch again after 10 days, if no mycotic infection; Can adopt the 3-4 stubble continuously, can adopt 0.2kg altogether for every bag, eliminate the bacterium tube that more renews after 50 days.

Claims

1. the efficient cultivation of a white ginseng bacterium is that preferred open country is joined bacterial strain separate tissue or spore separation inoculation on potato agar albumen test tube medium in vain, through thermophilic cultivate female kind; Again mother is planted and insert the glass buckwheat grain medium culture make and get original seed; Original seed is inserted medium that preparation handles well make and go into the canopy grand style of putting on the shelf behind the cultivated species, the management fruiting is characterized in that; The cultivated species medium has three kinds of prescriptions; Used barley corn, wheat berry, the buckwheat grain of cultivated species medium all must use 0.2% limewash to soak 40 hours, pick up with flushing with clean water only again with other material adapted, by ingredients by weight percentage be:

1. wheat berry 50%, broad-leaved weedtree sawdust 49%, gesso 1%; Or

2. barley corn 60%, broad-leaved weedtree sawdust 30%, wheat bran 9%, gesso 1%; Or

Batching is mixed all, add water by material-water ratio 1: 1.3-1.5 and stir, making water content is 55%-60%, and hand is pinched and do not dripped; Divide the bag of packing into, tighten mouth and insert wet sterilization pot pressurization 0.147MPa, temperature control keeps sterilization in 90 minutes, cooling naturally for 128 ℃; Pressure is reduced to the taking-up sack that boiled at 0 o'clock, when temperature is reduced to below 25 ℃, inserts the original seed make with sterile working, with direct put on the shelf 14-28 ℃ of the grand style temperature control of an immigration booth in non-drying adhesive tape sealing label inoculation eye back; Damping 80-90% carries out management of producing mushroom, adopts the first planting and joins bacterium in vain in 15-18 days; Manage and protect 10 days with method and adopt regrowth hair, adopt the 3-4 stubble continuously, eliminate the bacterium tube after 50 days.

2. the efficient cultivation of white ginseng bacterium as claimed in claim 1 is characterized in that, row's four eyed is adopted in the inoculation of cultivated species, inserts 20-25 grain original seed buckwheat grain for every.

3. the efficient cultivation of white ginseng bacterium as claimed in claim 1; It is characterized in that cultivated species inoculation 3 days punctures the exhaust of sealing compound cloth with 0.3cm diameter iron nail; Threw off in 8-9 days and seal adhesive plaster; And cut and scratch each 1 of sack 1.5cm osculum in mycelia haloing limit, both sides up and down at inoculation eye, form 12 fruiting eyes of three rows.

4. the efficient cultivation of white ginseng bacterium as claimed in claim 1 is characterized in that, the medium of female kind, original seed adopts pressure 0.147MPa, temperature 128-130 ℃ of steam pressure retorts sterilization 40-60 minute.