CN101665378B

CN101665378B - Mother seed carrier culture medium for culturing natural ganoderma and culturing method

Info

Publication number: CN101665378B
Application number: CN2009100675063A
Authority: CN
Inventors: 郭绍斌; 郭欣
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-09-04
Filing date: 2009-09-04
Publication date: 2011-09-28
Anticipated expiration: 2029-09-04
Also published as: CN101665378A

Abstract

The invention relates to a mother seed carrier culture medium for culturing natural ganoderma, comprising 2,500g of ground corn which 2g of monopotassium phosphate, 1g of magnesium sulfate, 110mg of vitamin B, 7.5g of gesso, 1.5g of lime and 500ml of water with temperature of 60-70 DEG C, wherein the ground corn is filtered by gauze after being dipped for 12h. By adopting an improved mother seed culture medium prescription, the mycelium is white, grows well and has the survival rate over 98 percent, and solves a problem that strains are difficult to preserve and enlarge. A method for culturing natural ganoderma by the mother seed carrier culture medium is characterized in that after-ripening and hibernation processing are carried out on cultured seeds after the strains sprout for 50-60 days. The invention succeeds in culturing instead of other materials in 2007 to obtain a culture medium with a diameter about 13 cm and succeeds in culturing on a short section of wooden clinker in 2009 to obtain a fruiting body with a diameter of 17-22 cm, which grows on a wooden section with the length of 15 cm; and the successful culturing of the natural ganoderma lays a solid foundation for single edible mushroom culture to go up to pillar industries.

Description

The wild Ganoderma cultivation is planted solid support medium and cultivating method with mother

Technical field

The present invention relates to a kind of mother culture media and cultivating method of wild Ganoderma cultivation usefulness.

Background technology

At present, mother culture media is made of potato 200g, glucose 20g, potassium primary phosphate 2g, sal epsom 0.5g, agar 20g, water 1000ml in the cultivation of glossy ganoderma, the pH value nature.Its female making processes of planting is: the dress test tube, and high pressure 30 minutes, the cooling of pendulum inclined-plane, temperature is controlled at below 30 ℃, and sterile cupboard connects bacterium, and 26-30 ℃ of sterilisable chamber cultivated, and tissue block survives after three days.Purify, extract most advanced and sophisticated 3mm, enlarged culturing covered with test tube about 12 days again, and check the mycelial growth situation every day between incubation period, and when long 1/3 area to the inclined-plane of mycelia, mycelia begins to wear out, and is all aging behind the mycelia full packages, have only locate about the 1cm of two ends pure white.Because the chamfered surface mycelia is hard, be difficult for stripping and slicing and separate, if careless, cause surperficial mycelia all to tick easily, bring a difficult problem to enlarged culturing.And up to the present, also no one else can do this stage of cultivation, makes bacterium and can not grow glossy ganoderma.

Summary of the invention

One of technical problem to be solved by this invention provides the mother culture media prescription that a kind of wild Ganoderma cultivating method relates to, solved original substratum aging easily, preserve difficulty and mycelia is not easily separated, the difficult problem that is difficult for enlarged culturing; Two of technical problem to be solved provides a kind of cultivating method of science, to turn out the glossy ganoderma of high rate of finished products.

For solving the problems of the technologies described above, the mother who the invention provides a kind of wild Ganoderma cultivation usefulness plants solid support medium, it is characterized in that: comprise that the corn 2500g that is cut soaks after 12 hours with gauze drainage, potassium primary phosphate 2g, sal epsom 1g, VB11 0mg, terra alba 7.5g, lime 1.5g, 60-70 ℃ water 500ml; Cultural method is: potassium primary phosphate, sal epsom and the VITMAIN B1 water with 60-70 ℃ is melted, be sprayed on corn quarrel, terra alba and the lime, stir, regulate moisture, make humidity reach 55-60%; Adorn 100 20 * 200mm test tubes then, install to test tube 3/5 place, test tube is an erectility, adorned test tube after, the thick 2.5 * 2.5cm sponge block of culture material surface cover 1cm is with the stifled test tube mouth of going up of cotton.

Sponge block is handled: use Semen Maydis powder 50g, earlier be modulated into pasty state with cold water, get 850ml boiling water then and reconstitute the corn starch adhesive, add biphosphate potassium 2g, sal epsom 0.5g, vitamin B15 mg after boiling again, after the cooling, sponge block was soaked 30 minutes, pulling drainage out does not get final product to not dripping, capping particle test tube surface, to prevent the culture material dehydration, the inoculation piece is difficult for surviving.

Utilize above-mentioned female method of planting solid support medium cultivation wild Ganoderma, comprise the female kind of wild collection and separate tissue, purify and enlarge, original seed is cultivated, cultivar is cultivated, cultivate down, it is characterized in that: after cultivar sends out bacterium good through time of 50-60 days, handle by being stranded bacterium and hibernation, when being stranded bacterium the cultivation bacterium being placed on aseptic bacterium chamber gets final product, 20 ℃ of temperature, substituting stuff cultivation needs 3-6 month, weak point is segment wood cultivated to need 6-8 month, next carry out hibernation, no matter be substituting stuff cultivation or short segment wood cultivated, the hibernation time is December to next year February, outdoor natural hibernation.

The present invention uses the mother culture media prescription after the improvement, mycelia is pure white, grow fine, surviving rate is more than 98%, a difficult problem that has solved the culture presevation difficulty and be difficult to enlarge, every female kind is changeed original seed 8-10 bottle (seed bottle of 500ml) or 8-10 bag (15 * 28cm polypropylene strain bag), the 2007's of wild Ganoderma material is cultivated successfully, 13cm left and right sides diameter, and pedlar's purchasing price is about 200 yuan every jin, a traditional unit of weight, the wooden grog of short section was cultivated successfully in 2009, the sporophore 17-22cm that the 15cm juggle grows, market value is about 500-700 unit, be family plant more than 10 times, the robe powder of kind is planted by family, about 160 yuan every jin, a traditional unit of weight, and the wild Ganoderma Lucidum in Changbai Mountain has just been cultivated success, former basic collection is less than spore powder, and its value is well imagined.The form of cultivating ganoderma all is a lateral growth, and phototropism is not obvious, all is fan-shaped more than 90%, and handle is short, and about about 3cm-10cm, color is the red-purple light.The cultivation success of wild Ganoderma Lucidum, this moves towards the column support type industry gradually to single edible fungus culturing and lays a solid foundation.

Embodiment

One, wild bacterium is gathered, separate tissue is female plants

Wild bacterium is collected in Changbai Mountain arteries and veins larch forest and felled the stub in 15 years, and the early May original hase forms the back and gathers, and obtains pure strain by the vegetative propagation separate tissue.

Two, purification enlarges

Female solid support medium prescription of planting: the corn 2500g that is cut soaks after 12 hours with gauze drainage, potassium primary phosphate 2g, sal epsom 1g, VITMAIN B1 10mg, terra alba 7.5g, lime 1.5g, 60-70 ℃ water 500ml; Cultural method is: potassium primary phosphate, sal epsom and the VITMAIN B1 water with 60-70 ℃ is melted, be sprayed on corn quarrel, terra alba and the lime, stir, regulate moisture, humidity 55-60% with the dried wood chip of hardwood broad-leaved; Adorn 100 20 * 200mm test tubes then, install to test tube 3/5 place, adorned test tube after, the thick 2.5 * 2.5cm sponge block of media surface lid 1cm is blocked the test tube mouth with cotton.Sponge block is handled, use Semen Maydis powder 50g, earlier be modulated into pasty state, get 850ml boiling water then and reconstitute the corn starch adhesive, add biphosphate potassium 2g, sal epsom 0.5g, VITMAIN B1 5mg after boiling again with cold water, after the cooling, sponge block was soaked 30 minutes, pull drainage (do not drip and get final product) out, capping particle test tube surface, to prevent the culture material dehydration, the inoculation piece is difficult for surviving.Every female kind is changeed original seed 8-10 bottle (seed bottle of 500ml) or 8-10 bag (15 * 28cm polypropylene strain bag).

Three, original seed is cultivated

Begin to do original seed October, and pedigree seed culture medium is filled a prescription by weight percentage and is: Semen Maydis powder 28%, broad-leaved wood chip 70%, gypsum 1.5%, lime 0.3%, potassium primary phosphate 0.2%, pH value 7-8; Normal-pressure sterilization, 100 ℃ 12 hours, autoclaving 121-126 degree centigrade 2.5 hours, be cooled to below 30 ℃, sterilisable chamber or sterile cupboard inoculation are placed the sterile culture chambers temp and are controlled at 26-30 ℃, after seven days, bottle or bag were covered with in ventilation every day 20 minutes in 50-60 days, 30 bags of every bottle of original seed switching cultivars.

Four, cultivar is cultivated

Next year begins to do cultivar January, and the cultivar substratum is filled a prescription by weight percentage and is: broad-leaved wood chip 78%, Semen Maydis powder 20%, gypsum 1.5%, lime 0.5%, pH value 8-9, humidity 55-60%;

Wood chip 78%, Semen Maydis powder 15%, wheat bran 5%, gypsum 1.5%, lime 0.5%, pH value 8-9, humidity 55-60%;

Wood chip 78%, Semen Maydis powder 15%, rice chaff 5%, gypsum 1.5%, lime 0.5%, pH value 8-9, humidity 55-60%;

Wood chip 78%, Semen Maydis powder 15%, soya bean stem meal 5%, gypsum 1.5%, lime 0.5%, pH value 8-9, humidity 55-60%;

Wood chip 78%, Semen Maydis powder 15%, broad-leaved leaf powder 5%, gypsum 1.5%, lime 0.5%, pH value 8-9, humidity 55-60%;

Normal-pressure sterilization, 100 ℃ of temperature were kept 10-12 hour.Autoclaving, was kept 2.5 hours by temperature 121-126 ℃.After taking the dish out of the pot, be cooled to 28-30 ℃, sterilisable chamber or sterile cupboard connect bacterium then, the sterile culture chamber, and 25-30 ℃, first half of the month, ventilated every day 20 minutes, behind the first quarter moon, strengthen ventilation, ventilated sooner or later every day 30 minutes, cover with bag about 50 days.

Cultivation can be adopted substituting stuff cultivation or the short segment wood cultivated two kinds of methods of grog.After cultivar sends out bacterium good through time of 50-60 days, handle by being stranded bacterium and hibernation, when being stranded bacterium the cultivation bacterium is placed on aseptic bacterium chamber and gets final product, 20 ℃ of temperature, substituting stuff cultivation needs 3-6 month, weak point is segment wood cultivated to need 6-8 month, next no matter carry out hibernation, be substituting stuff cultivation or short segment wood cultivated, at northern area, the hibernation time is December to next year February, and outdoor natural hibernation gets final product.The time of doing the cultivation bacterium should be flexible, and the tired bacterium time of substituting stuff cultivation is short, and the cultivation bacterium is just cooked evening, and the time of short segment wood cultivated tired bacterium is long, just early does bacterium.

Five, cultivate down

Early May is ground down, and planting type has two kinds, and a kind of is the sylvan life ecological cultivation, pseudo-wild cultivating just, and another kind is conventional greenhouse cultivation, the latter easily gathers the robe powder.During the sylvan life ecological cultivation, employing is advisable towards tailo towards tailo or west towards tailo, east, spacing between bag and bag or section and the section will be controlled at more than the 15cm, dig the cave and bury bag or section, smooth native rear surface and cover needle leaf and broad-leaved leaf each half, thickness 2cm, atmospheric moisture 85%, illumination range 30%.When adopting conventional greenhouse cultivation, bag and bag or section will be controlled at 15cm with spacing between the section, be advisable with silty loam, the cultivation section that 15cm is high or bag, the soil of covering 2/3,1/3 weathering sand or river sand, middle fine sand is advisable, 20 ℃ of temperature, atmospheric moisture 85%.A plurality of original hases appear in juggle that has or bag, stay to go for a short time greatly, guarantee that nutrition concentrates, and help improving output, plant from mother and cultivate glossy ganoderma and gather and need 2 years.

Claims

1. wild Ganoderma cultivation is characterized in that with female solid support medium of planting: comprise that the corn 2500g that is cut soaks after 12 hours with gauze drainage, potassium primary phosphate 2g, sal epsom 1g, VITMAIN B1 10mg, terra alba 7.5g, lime 1.5g, 60-70 ℃ water 500ml; Cultural method is: potassium primary phosphate, sal epsom and the VITMAIN B1 water with 60-70 ℃ is melted, be sprayed on corn quarrel, terra alba and the lime, stir, regulate moisture, make humidity reach 55-60%; Adorn 100 20 * 200mm test tubes then, install to test tube 3/5 place, test tube is an erectility, adorned test tube after, the thick 2.5 * 2.5cm sponge block of culture material surface cover 1cm is with the stifled test tube mouth of going up of cotton;

Sponge block is handled: use Semen Maydis powder 50g, earlier be modulated into pasty state with cold water, get 850ml boiling water then and reconstitute the corn starch adhesive, add biphosphate potassium 2g, sal epsom 0.5g, VITMAIN B1 5mg after boiling again, after the cooling, sponge block was soaked 30 minutes, pull drainage out to not dripping, capping particle test tube surface, to prevent the culture material dehydration, the inoculation piece is difficult for surviving.

2. utilize the described female wild Ganoderma cultivating method of planting solid support medium of claim 1, comprise the female kind of wild collection and separate tissue, purify and enlarge, original seed is cultivated, cultivar is cultivated, cultivate down, it is characterized in that: after cultivar sends out bacterium good through time of 50-60 days, handle by being stranded bacterium and hibernation, when being stranded bacterium the cultivation bacterium is placed on aseptic bacterium chamber, 20 ℃ of temperature, substituting stuff cultivation needs 3-6 month, weak point is segment wood cultivated to need 6-8 month, next carry out hibernation, no matter be substituting stuff cultivation or short segment wood cultivated, the hibernation time is December to next year February, outdoor natural hibernation.