CN104686198A - Method for cultivation of edible tree funguses through mulberry branches - Google Patents
Method for cultivation of edible tree funguses through mulberry branches Download PDFInfo
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- CN104686198A CN104686198A CN201510107782.3A CN201510107782A CN104686198A CN 104686198 A CN104686198 A CN 104686198A CN 201510107782 A CN201510107782 A CN 201510107782A CN 104686198 A CN104686198 A CN 104686198A
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Abstract
Disclosed is a method for cultivation of edible tree funguses through mulberry branches. The method comprises the following steps that (1) preparation of a culture medium is conducted; (2) matched materials are packaged with bags and sterilized; (3) inoculation is conducted; (4) cultivation of the funguses is conducted; (5) fruiting management is conducted. The culture medium comprises, by weight, 56-66 parts of mulberry branch scraps, 10-20 parts of Chinese magnoliavine fruit dry powder, 5-10 parts of garlic dry powder, 5-10 parts of cinnamon dry powder, 5-10 parts of momordica cochinchinensis dry powder, 10-20 parts of bran, 10-20 parts of cottonseed hulls, 1-3 parts of sugars, 1-3 parts of lime, 0.2-0.7 part of calcium superphosphate and 0.1-0.3 part of magnesium sulfate. The method for cultivation of the edible tree funguses through the mulberry branches solves the problem that a large number of sundry funguses exist when the edible tree funguses are cultivated in the south, and is lower in rate of the sundry funguses in the edible tree funguses and higher in pest resistance and disease resistance.
Description
Technical field
The present invention relates to a kind of agricultural product production technical field, be specifically related to a kind of method of ramulus mori fungus cultivation.
Background technology
Auricularia auriculajudae, primary growth is at China and Japan.Auricularia auriculajudae not only can be wild but also can cultivate, and planting fruit body is ear
,lobate or cup-shaped
,thin
,edge wave wave-like, wide 3-10 centimetre, thick 2 millimeter, the short handle raw with side or narrow thin facies posterior hepatis are bonded in matrix.Auricularia auriculajudae color and luster is dark brown, and quality softness is in colloidal substance, thin and flexible, translucent time moistening, shrinks to become crisp hard cutin and be similar to keratin time dry.Delicious flavour, can element can meat or fish, nutritious.
Auricularia auriculajudae belongs to S middle warm type fungi.Mycelia all can grow between 6 ~ 36 DEG C, but with 22 ~ 32 DEG C of optimums; 15 ~ 27 DEG C all can be broken up for entity, but with 20 ~ 24 DEG C of optimums.Fungus mycelium all can grow in the planting material of water content 60% ~ 70% and Duan Muzhong, requires that ear wood water content reaches more than 70%, relative air humidity 90% ~ 95% during fruit-body formation.Mycelia in the dark can normal growth, and the sporophore growth phase needs the intensity of illumination of 250 ~ 1000lx.Auricularia auriculajudae is aerobic fungi, pH5 ~ 5.6 optimum.Fungus cultivation method has segment wood cultivated multiple with plastic sack substituting stuff cultivation etc.
People is segment wood cultivated is with wood, and make some sections, then hole above, implant bacterium, auricularia auriculajudae just can grow out in hole, can pluck after auricularia auriculajudae maturation.
Plastic sack substituting stuff cultivation is with wood chip
, stalkfor raw material, utilize plastic sack splendid attire, often packed 0.5 kg dry matter, through sterilizing
,inoculation
,bacteria, puts at field the earth
,orchard sylvan life goes out ear.
Domestic patent database also discloses some fungus cultivation technology, such as:
Such as: a kind of [title] method utilizing mulberry branch fungus cultivation, [application number] CN201210281996.9, [applying date] 2012.08.09, the implantation methods of a kind of auricularia auriculajudae, the raw material composition of the formulation consists weight portion of mulberry branch fungus cultivation: mulberry branch 80-85
,rice bran 1-5
,wheat bran 1-5
,corn flour 1-5
,silkworm excrement 1-5
,land plaster 1-3
,pulverized limestone 0.1-1
,sugar 1-3
,cotton dregs 1-5
,analysis for soybean powder 1-3
,calcium carbonate 0.1-1.Comprise (1) spice; (2) pack and sterilizing; (3) take the dish out of the pot
,cooling; (4) inoculate; (5) hair tube reason; (6) ear field prepares; (7) ear grand style is urged; (8) steps such as syrinx reason are gone out.This invention technology is simple and easy
,cost is low
,instant effect.
Again such as: [title] a kind of technology utilizing bacterium bag cultivating auricularia auriculajudae, [patent No.] CN00124364.0, [applying date] 2000.09.11, disclose a kind of technology utilizing bacterium bag cultivating auricularia auriculajudae, comprises and send out bacterium and go out ear two steps, wherein send out bacterium technique and comprise charging
,sealing
,intubate
,sterilizing
,inoculation and cultivation, going out ear technique is that the bacterium bag covering with mycelia is moved to agaric growing field or agaric growing room, and built into wall type structure, this invention adds the gas permeability of bag, make absorb enough oxygen during mycelial growth, the mycelium growing period of comparable prior art shortens 1/3 ~ 1/2, also can increase bacterium bag and amass, be easy to management, and effectively can prevent the intrusion of germ and insect pest, utilization of area rate 4 ~ 5 times can be improved simultaneously, improve biologicak efficiency 20 ~ 50%, unit are increase yield 6 ~ 7 times.
But above fungus cultivation technology is not suitable for using in south, because woodear belongs to cold-resistant To Be Protected from Heat type mushroom, too high temperature is unfavorable for the growth of woodear, and the condition of southern high-temperature high humidity, more be conducive to spore germination and the growth and breeding of the multiple moulds such as Trichoderma viride, if at southern fungus cultivation, certainly exist the problem that miscellaneous bacteria is grown thickly.
About in the more problem of south plantation auricularia auriculajudae miscellaneous bacteria, some also inventor also provide many solutions:
More typically, the cultivation method of [title] a kind of southern area woodear, [application number] CN201410115146.0, [applying date] 2014.03.25, disclose the cultivation method of a kind of southern area woodear, comprise: (1) system rod: planting material is loaded in pocket, obtained charge bar; (2) inoculate: in mid-June to early July, inoculate after charge bar sterilizing, after having inoculated, increase cover control oxygen bag outward at charge bar; (3) bacterium is sent out: the charge bar completing bagging is moved into outdoor booth, the parallel adjacent stacking of bacterium rod; Between bacteria developing period, take to rest, do not carry out turning operation; Utilize spraying cooling in conjunction with forced ventilation, control canopy temperature 25 ~ 33 DEG C, relative moisture 60 ~ 70%, sends out bacterium to mid or late August to early September and completes; (4) acanthopore urges ear; (5) grand style goes out ear.
This invention solves the more problem of miscellaneous bacteria, and its core is inoculation to fix on mid-June to early July, and at this time southern weather is especially applicable to auricularia auriculajudae growth, can effectively reduce miscellaneous bacteria rate.But the method for this fungus cultivation, by the restriction of time, can make the producer miss the busy season of auricularia auriculajudae, affect selling price.
Therefore be necessary very much to research and develop a kind of new fungus cultivation technology, improve the problem that south plantation auricularia auriculajudae miscellaneous bacteria is more better.
Summary of the invention
The object of the invention is the more problem of miscellaneous bacteria solving current southern fungus cultivation existence, provide a kind of ramulus mori fungus cultivation method that significantly can suppress varied bacteria growing.
The method of ramulus mori fungus cultivation of the present invention, comprises the following steps:
A) medium is made: culture medium prescription is as follows: ramulus mori bits 56-66 part
,schisandra sphenanthera Rehd.et WilS dry powder 10-20 part
,garlic dry powder 5-10 part
,cassia bark dry powder 5-10 part
,wood small pin for the case dry powder 5-10 part, wheat bran 10-20 part
,cotton seed hulls 10-20 part
,sugar 1-3 part
,lime 1-3 part
,superphosphate 0.2-0.7 part
,magnesium sulfate 0.1-0.3 part;
Manufacturing process is as follows: the ramulus mori bits taking respective amount
,schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,wood small pin for the case dry powder
,wheat bran
,cotton seed hulls is mixed into major ingredient, then using the sugar as clout
,lime
,superphosphate
,magnesium sulfate pours in major ingredient after being dissolved in a small amount of water, turns to water content reach 60-65% while add clear water limit; The pH value of adjustment agaric culture medium is between 5-6;
B) batching pack sterilizing: the medium of step a gained batching is loaded in pocket, obtained bacterium rod; To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally;
C) inoculate: when bacterium rod material temperature drop is to less than 25 DEG C, auricularia auriculajudae original seed is inoculated into bacterium rod surface, increases cover control oxygen bag outward at bacterium rod;
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, the parallel adjacent stacking of bacterium rod; Between bacteria developing period, take to rest, do not carry out turning operation; Utilize spraying cooling in conjunction with forced ventilation, control canopy temperature 26-32 DEG C, relative moisture 60-70%, within 20-30 days, send out bacterium after inoculation and complete;
E) syrinx reason is gone out: the bacterium bag covering with mycelia is moved to agaric growing field or agaric growing room, is built into wall type structure; Removing control oxygen bag, after bacterium rod is kept 10-15 days, carry out punching to bacterium rod and process, then bacteria urges ear 7-10 days, treats that earhole mycelia recovers, then carries out grand style and go out ear; When ear Buds formation, morning and evening relative moisture control at 90-95%, noon, relative moisture controlled at 60-65%, treated that about 10 days auricularia auriculajudae fruit bodys are ripe, can gather.
Further illustrate as of the present invention, in step a, described ramulus mori can be fresh mulberry bar
,branch also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched is pulled out after will being soaked in water 3-5 hour to drain away the water and just applied, and ensureing that ramulus mori considers water content to be worth doing is 6-10%.
Further illustrate as of the present invention, in step a, Schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,the preparation method of wood small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit
,garlic
,cassia bark powder
,wood small pin for the case powder, adopt volumetric concentration to be ethanol leaching 2-3 time of 60-80% respectively, each 1-3 hour, obtains extract, more namely gained extract removing ethanol is obtained the dry powder of correspondence.
Further illustrate as of the present invention, in step b, medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour; Every bag approximately loads height 18-20cm.
Further illustrate as of the present invention, in step c, seeded process operates on aseptic operating platform, needs in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, before inoculation, and also will to the table top in operating desk
,the hand medicinal alcohol of article and operator carries out disinfection.
Further illustrate as of the present invention, in steps d, the ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.
Further illustrate as of the present invention, in step e, bacterium rod earhole quantity is 150-180.
Further illustrate as of the present invention, in step e, every damp auricularia auriculajudae stops watering after gathering, and allows solar radiation 3-5 days, make bacterium rod dry tack free, oxygen enters from crack, impels mycelia restoration ecosystem, rewater management, and after 10-15 days, fruit body is ripe, two damp auricularia auriculajudaes of just can gathering.
The method of ramulus mori fungus cultivation of the present invention has following technological progress compared to existing technology:
(1) make in medium process, consider to be worth doing with ramulus mori
,schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,wood small pin for the case dry powder
,wheat bran
,cotton seed hulls is mixed into major ingredient.Wherein Schisandra sphenanthera Rehd.et WilS
,the dry powder such as garlic extract from the plant of correspondence, containing natural bacteriostatic composition.The present invention by four kinds of Chinese medicine dry powder with the use of, make the synergy between them, not only effectively can suppress the growth of miscellaneous bacteria in the situation of lower usage amount, and to auricularia auriculajudae growth almost have no negative effect, be a kind of Chinese medicine miscellaneous bacteria inhibitor of high selectivity.This Chinese medicine miscellaneous bacteria inhibitor directly adds in agaric culture medium the effect can played and suppress miscellaneous bacteria.
(2) China be breed silkworms in the world and mulberry cultivation area maximum
country, mulberry tree plantation nationwide, mulberry tree, except picking leaves is bred silkworms, still has a large amount of ramulus moris to waste as sericulture discarded object every year.The present invention develops ramulus mori fungus cultivation technology, using ramulus mori bits as auricularia auriculajudae main medium, not only takes full advantage of accessory substance resource the abundantest in Sericultural production process, also for the cultivation matrix of auricularia auriculajudae provides abundant raw material sources.
(3) the present invention also adds magnesium sulfate in the medium, and magnesium elements can promote mycelial growth, improves in fruiting body yield, and has a certain impact to medium and small grade fruit body size and number; After adding magnesium sulfate, the biological transformation ratio of auricularia auriculajudae significantly improves, and output is also more stable.
Embodiment
Below in conjunction with embodiment, rice cultivation method of the present invention is described further.
Embodiment 1: the method for ramulus mori fungus cultivation of the present invention, the edible fungus selected is No. one, Shanghai ear.
A) medium is made: culture medium prescription is as follows: ramulus mori considers 56 parts to be worth doing
,10 parts, Schisandra sphenanthera Rehd.et WilS dry powder
,5 parts, garlic dry powder
,5 parts, cassia bark dry powder
,5 parts, wood small pin for the case dry powder, 10 parts, wheat bran
,cotton seed hulls 10 parts
,sugar 1 part
,1 part, lime
,superphosphate 0.2 part
,0.1 part, magnesium sulfate;
Manufacturing process is as follows: the ramulus mori bits taking respective amount
,schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,wood small pin for the case dry powder
,wheat bran
,cotton seed hulls is mixed into major ingredient, then using the sugar as clout
,lime
,superphosphate
,magnesium sulfate pours in major ingredient after being dissolved in a small amount of water, turns to water content reach 60% while add clear water limit; The pH value of adjustment agaric culture medium is 5;
Described ramulus mori can be fresh mulberry bar
,branch also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched to be soaked in water pulling out after 3 hours drain away the water just apply, ensure ramulus mori bits water content be 6%.
Schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,the preparation method of wood small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit
,garlic
,cassia bark powder
,wood small pin for the case powder, adopt respectively volumetric concentration be 60% ethanol leach 2 times, each 1 hour, obtain extract, more namely gained extract removing ethanol obtained corresponding dry powder.
B) batching pack sterilizing: the medium of step a gained batching is loaded in pocket, obtained bacterium rod; To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally;
Medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour; Every bag approximately loads height 18-20cm, is specifically highly determined on a case-by-case basis.
C) inoculate: when bacterium rod material temperature drop is to less than 25 DEG C, auricularia auriculajudae original seed is inoculated into bacterium rod surface, increases cover control oxygen bag outward at bacterium rod;
Seeded process operates on aseptic operating platform, needs in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, and the concrete time is determined on a case-by-case basis, before inoculation, and also will to the table top in operating desk
,the hand medicinal alcohol of article and operator carries out disinfection.
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, and the ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.The parallel adjacent stacking of bacterium rod; Between bacteria developing period, take to rest, do not carry out turning operation; Utilize spraying cooling in conjunction with forced ventilation, control canopy temperature 26-32 DEG C, relative moisture 60-70%, within 20-30 days, send out bacterium after inoculation and complete;
E) syrinx reason is gone out: the bacterium bag covering with mycelia is moved to agaric growing field or agaric growing room, is built into wall type structure; Removing control oxygen bag, after bacterium rod is kept 10-15 days, carries out punching process to bacterium rod, and it is that 150-180 is individual that the excellent earhole quantity of bacterium is beaten by institute; Then bacteria urges ear 7-10 days, treats that earhole mycelia recovers, then carries out grand style and go out ear; When ear Buds formation, morning and evening relative moisture control at 90-95%, noon, relative moisture controlled at 60-65%, treated that about 10 days auricularia auriculajudae fruit bodys are ripe, can gather.
Every damp auricularia auriculajudae stops watering after gathering, and allows solar radiation 3-5 days, and make bacterium rod dry tack free, oxygen enters from crack, impels mycelia restoration ecosystem, and rewater management, fruit body maturation after 10-15 days, two damp auricularia auriculajudaes of just can gathering.
Below the auricularia auriculajudae result of the test of enforcement 1:
table 1, the test result of embodiment 1
| Bacterium rod yield rate (%) | Auricularia auriculajudae output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant 、Disease resistance |
| 25 | 45 | 0.5 | Nothing | Nothing | By force |
Embodiment 2: the method for ramulus mori fungus cultivation of the present invention, the edible fungus selected is No. one, Shanghai ear.
A) medium is made: culture medium prescription is as follows: ramulus mori considers 66 parts to be worth doing
,20 parts, Schisandra sphenanthera Rehd.et WilS dry powder
,10 parts, garlic dry powder
,10 parts, cassia bark dry powder
,10 parts, wood small pin for the case dry powder, 20 parts, wheat bran
,cotton seed hulls 20 parts
,sugar 3 parts
,3 parts, lime
,superphosphate 0.7 part
,0.3 part, magnesium sulfate;
Manufacturing process is as follows: the ramulus mori bits taking respective amount
,schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,wood small pin for the case dry powder
,wheat bran
,cotton seed hulls is mixed into major ingredient, then using the sugar as clout
,lime
,superphosphate
,magnesium sulfate pours in major ingredient after being dissolved in a small amount of water, turns to water content reach 65% while add clear water limit; The pH value of adjustment agaric culture medium is 6;
Described ramulus mori can be fresh mulberry bar
,branch also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched to be soaked in water pulling out after 5 hours drain away the water just apply, ensure ramulus mori bits water content be 10%.
Schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,the preparation method of wood small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit
,garlic
,cassia bark powder
,wood small pin for the case powder, adopt respectively volumetric concentration be 80% ethanol leach 3 times, each 3 hours, obtain extract, more namely gained extract removing ethanol obtained corresponding dry powder.
Step b)
,c)
,d)
,e) identical with case study on implementation 1.
Below the auricularia auriculajudae result of the test of enforcement 2:
table 2, the test result of embodiment 2
| Bacterium rod yield rate (%) | Auricularia auriculajudae output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant 、Disease resistance |
| 30 | 52 | 1 | Nothing | Nothing | By force |
Embodiment 3: the method for ramulus mori fungus cultivation of the present invention, the edible fungus selected is No. one, Shanghai ear.
A) medium is made: culture medium prescription is as follows: ramulus mori considers 60 parts to be worth doing
,15 parts, Schisandra sphenanthera Rehd.et WilS dry powder
,8 parts, garlic dry powder
,8 parts, cassia bark dry powder
,80 parts, wood small pin for the case dry powder, 8 parts, wheat bran
,cotton seed hulls 15 parts
,sugar 2 parts
,2 parts, lime
,superphosphate 0.5 part
,0.2 part, magnesium sulfate;
Manufacturing process is as follows: the ramulus mori bits taking respective amount
,schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,wood small pin for the case dry powder
,wheat bran
,cotton seed hulls is mixed into major ingredient, then using the sugar as clout
,lime
,superphosphate
,magnesium sulfate pours in major ingredient after being dissolved in a small amount of water, turns to water content reach 62% while add clear water limit; The pH value of adjustment agaric culture medium is 5;
Described ramulus mori can be fresh mulberry bar
,branch also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched is pulled out after will being soaked in water 3-5 hour to drain away the water and just applied, and ensureing that ramulus mori considers water content to be worth doing is 8%.
Schisandra sphenanthera Rehd.et WilS dry powder
,garlic dry powder
,cassia bark dry powder
,the preparation method of wood small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit
,garlic
,cassia bark powder
,wood small pin for the case powder, adopt respectively volumetric concentration be 70% ethanol leach 3 times, each 2 hours, obtain extract, more namely gained extract removing ethanol obtained corresponding dry powder.
Step b)
,c)
,d)
,e) identical with case study on implementation 1.
Below the auricularia auriculajudae result of the test of enforcement 3:
table 3, the test result of embodiment 3
| Bacterium rod yield rate (%) | Auricularia auriculajudae output (g/ rod) | Bacterial contamination rate (%) | Disease condition | Insect pest situation | Pest-resistant 、Disease resistance |
| 28 | 46 | 1 | Nothing | Nothing | By force |
Comparative example: the method for the ramulus mori fungus cultivation of prior art, the edible fungus selected equally is No. one, Shanghai ear.
Comparative example comprises the following steps: the raw material composition of the formulation consists weight portion of mulberry branch fungus cultivation: mulberry branch 80
,rice bran 3
,wheat bran 3
,corn flour 3
,silkworm excrement 3
,land plaster 2
,pulverized limestone 0.5
,sugar 2
,cotton dregs 3
,analysis for soybean powder 3
,calcium carbonate 0.5.Cultivation comprises (1) spice; (2) pack and sterilizing; (3) take the dish out of the pot
,cooling; (4) inoculate; (5) hair tube reason; (6) ear field prepares; (7) ear grand style is urged; (8) steps such as syrinx reason are gone out.
Below the auricularia auriculajudae result of the test of comparative example:
table 4, the test result of comparative example
Integrated comparative embodiments of the invention 1-3 and comparative example obtain following result:
table 5, Comprehensive Correlation result
| Object | Bacterium rod yield rate | Auricularia auriculajudae output | Bacterial contamination rate | Disease condition | Insect pest situation | Pest-resistant 、Disease resistance |
| The present invention | High | High | Low | Gently | Gently | By force |
| Conventional art | Low | Low | High | Seriously | Seriously | Weak |
Comprehensive Correlation is visible, and method of the present invention compares conventional art, and bacterial contamination rate significantly reduces
,resistant to diseases and insects is stronger, is highly suitable for the universal use in south.
Claims (8)
1. a method for ramulus mori fungus cultivation, is characterized in that, comprises the following steps:
A) medium is made: culture medium prescription is as follows: ramulus mori bits 56-66 part, Schisandra sphenanthera Rehd.et WilS dry powder 10-20 part, garlic dry powder 5-10 part, cassia bark dry powder 5-10 part, wooden small pin for the case dry powder 5-10 part, wheat bran 10-20 part, cotton seed hulls 10-20 part, sugared 1-3 part, lime 1-3 part, superphosphate 0.2-0.7 part, magnesium sulfate 0.1-0.3 part;
Manufacturing process is as follows: take the ramulus mori bits of respective amount, Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder, wheat bran, cotton seed hulls be mixed into major ingredient, pour into as the sugar of clout, lime, superphosphate, magnesium sulfate in major ingredient after being dissolved in a small amount of water again, turn to water content reach 60-65% while add clear water limit; The pH value of adjustment agaric culture medium is between 5-6;
B) batching pack sterilizing: the medium of step a gained batching is loaded in pocket, obtained bacterium rod; To heat under bacterium rod is placed in condition of normal pressure sterilizing, keep 10-l2 hour when the temperature stabilization in bag is at 1O0 DEG C, then cooling naturally;
C) inoculate: when bacterium rod material temperature drop is to less than 25 DEG C, auricularia auriculajudae original seed is inoculated into bacterium rod surface, increases cover control oxygen bag outward at bacterium rod;
D) cultivation: just the bacterium rod of step c gained moves into outdoor booth, the parallel adjacent stacking of bacterium rod; Between bacteria developing period, take to rest, do not carry out turning operation; Utilize spraying cooling in conjunction with forced ventilation, control canopy temperature 26-32 DEG C, relative moisture 60-70%, within 20-30 days, send out bacterium after inoculation and complete;
E) syrinx reason is gone out: the bacterium bag covering with mycelia is moved to agaric growing field or agaric growing room, is built into wall type structure; Removing control oxygen bag, after bacterium rod is kept 10-15 days, carry out punching to bacterium rod and process, then bacteria urges ear 7-10 days, treats that earhole mycelia recovers, then carries out grand style and go out ear; When ear Buds formation, morning and evening relative moisture control at 90-95%, noon, relative moisture controlled at 60-65%, treated that about 10 days auricularia auriculajudae fruit bodys are ripe, can gather.
2. the method for ramulus mori fungus cultivation according to claim 1, is characterized in that: in step a, and described ramulus mori can be fresh mulberry bar, branch, also can be the ramulus mori parched; Wherein, fresh ramulus mori is directly applied, and the ramulus mori parched is pulled out after will being soaked in water 3-5 hour to drain away the water and just applied, and ensureing that ramulus mori considers water content to be worth doing is 6-10%.
3. the method for ramulus mori fungus cultivation according to claim 1, it is characterized in that: in step a, the preparation method of Schisandra sphenanthera Rehd.et WilS dry powder, garlic dry powder, cassia bark dry powder, wooden small pin for the case dry powder is as follows: get Chinese Magnolivine Fruit, garlic, cassia bark powder, wooden small pin for the case powder, respectively adopt volumetric concentration be 60-80% ethanol leaching 2-3 time, each 1-3 hour, obtain extract, more namely gained extract removing ethanol is obtained corresponding dry powder.
4. the method for ramulus mori fungus cultivation according to claim 1, is characterized in that: in step b, and medium batching is dispensed into rapidly in polypropylene bacterium bag after mixing, in order to avoid windrow is long for standing time, causes and becomes sour; Every bag approximately loads height 18-20 cm.
5. according to the method for the arbitrary described ramulus mori fungus cultivation of claim 1-4, it is characterized in that: in step c, seeded process operates on aseptic operating platform, need in advance to carry out ultraviolet disinfection 0.5-1h to operating desk, before inoculation, also to carry out disinfection to the hand medicinal alcohol of the table top in operating desk, article and operator.
6. the method for ramulus mori fungus cultivation according to claim 5, is characterized in that: in steps d, and the ceiling of described booth covers black and white film, arranges sunshade net above ceiling, erection spray equipment.
7. the method for ramulus mori fungus cultivation according to claim 6, is characterized in that: in step e, and bacterium rod earhole quantity is 150-180.
8. the method for ramulus mori fungus cultivation according to claim 7, it is characterized in that: in step e, every damp auricularia auriculajudae stops watering after gathering, allow solar radiation 3-5 days, make bacterium rod dry tack free, oxygen enters from crack, impel mycelia restoration ecosystem, rewater management, and after 10-15 days, fruit body is ripe, two damp auricularia auriculajudaes of just can gathering.
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|---|---|---|---|
| CN201510107782.3A CN104686198A (en) | 2015-03-12 | 2015-03-12 | Method for cultivation of edible tree funguses through mulberry branches |
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|---|---|---|---|
| CN201510107782.3A CN104686198A (en) | 2015-03-12 | 2015-03-12 | Method for cultivation of edible tree funguses through mulberry branches |
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| Publication Number | Publication Date |
|---|---|
| CN104686198A true CN104686198A (en) | 2015-06-10 |
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| CN201510107782.3A Pending CN104686198A (en) | 2015-03-12 | 2015-03-12 | Method for cultivation of edible tree funguses through mulberry branches |
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| CN107434723A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | A kind of planting material for improving rainbow conk yield |
| CN108575555A (en) * | 2018-05-02 | 2018-09-28 | 习水县富农菌业种植农民专业合作社 | A kind of implantation methods of black fungus |
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| CN107434723A (en) * | 2016-05-27 | 2017-12-05 | 临桂铭泰果蔬农民专业合作社 | A kind of planting material for improving rainbow conk yield |
| CN106810357A (en) * | 2016-12-30 | 2017-06-09 | 新昌县拜特夫农业科技有限公司 | The method of ramulus mori fungus cultivation |
| CN108575555A (en) * | 2018-05-02 | 2018-09-28 | 习水县富农菌业种植农民专业合作社 | A kind of implantation methods of black fungus |
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