CN105418291A - Edible fungus culture medium - Google Patents
Edible fungus culture medium Download PDFInfo
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- CN105418291A CN105418291A CN201511023680.XA CN201511023680A CN105418291A CN 105418291 A CN105418291 A CN 105418291A CN 201511023680 A CN201511023680 A CN 201511023680A CN 105418291 A CN105418291 A CN 105418291A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05D3/00—Calcareous fertilisers
Abstract
The invention discloses an edible fungus culture medium. The medium is composed of the following components: mulberry twig chips, walnut shell, peanut shell, edible fungus residues, oat residues, grosvenor momordica fruit residues, bamboo leaf residues, lime, gypsum, and additive; wherein the additive is composed of the following components in parts by weight: 20 to 25 parts of edible fungus extract, 10 to 20 parts of oat extract, 10 to 15 parts of grosvenor momordica fruit extract, 5 to 7 parts of bamboo leaf extract, 6 to 8 parts of chrysanthemum extract, 1 to 3 parts of sodium base bentonite, 0.3 to 0.5 parts of talcum powder, 0.1 to 0.3 part of magnesium sulfate, 0.2 to 0.4 part of calcium sulfate, 0.1 to 0.3 part of zinc sulfate, 0.01 to 0.03 part of sodium selenite, and 0.2 to 0.4 part of potassium dihydrogen phosphate. The raw materials are fully utilized, the culture medium can provide nutrients and also has a certain antibacterial function, and thus the edible fungus yield and polysaccharide content are both improved.
Description
Technical field
The present invention relates to a kind of agaric culture medium matter, belong to technical field of edible fungi production.
Background technology
Auricularia auriculajudae, has another name called black fungus, brightness program, is under the jurisdiction of Basidiomycetes, Auriculariale, Auriculariaceae, is a kind of edible mushrooms that Chinese is often eaten.Its property is put down, and taste is sweet, have enriching yin strong, enrich blood and invigorate blood circulation, improve body immunity, alleviate the effects such as arteriosclerosis.This gives the credit to it and contains the nutrient substance such as the element such as protein, fat, polysaccharide and calcium, phosphorus, iron and carotene, VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate, nicotinic acid, phosphatide, sterol, and multiple human body essential amino acid.In recent years, along with people rise hastily to the demand of auricularia auriculajudae, the emphasis of research little by little concentrates in agaric culture medium aspect by research worker.
At present, agaric culture medium mainly using corn cob, wood chip, cotton seed hull, wheat bran, agricultural crop straw etc. as culture material, terra alba, white sugar etc. as batching, and through preparing burden, adding water, stir, pack, the step such as sterilizing is made.Although culture material corn cob, wood chip, cotton seed hull, wheat bran, agricultural crop straw etc. are rich in xylogen or Mierocrystalline cellulose, but their protein contents are little, flavonoid, alkaloid are extremely low, nutrition is few, thus the auricularia auriculajudae causing substratum to be cultivated is second-rate, and then make the deficiency in economic performance of auricularia auriculajudae.In order to solve this technical problem, research worker, through years of researches, have found answer from research agaric culture medium matter.As Chinese patent CN104817354A discloses " a kind of agaric culture medium matter and preparation method thereof ", this culture medium is formed by the preparation of raw material of following weight part: seeds of a tung oil tree wood chip 80-120 part, seeds of a tung oil tree shell 40-60 part, bagasse 20-30 part, lime 1-3 part, gypsum 1-3 part, nutritional additive 3-8 part.Wherein, the every 1000g of nutritional additive is made up of following raw material: manganous sulfate 0.2-0.5 part, ammonium molybdate 0.2-0.5 part, calcium superphosphate 0.5-1.5 part, copper sulfate 0.3-0.8 part, zinc chloride 0.2-0.6 part, phosphoric acid selenium potassium 0.1-0.5 part, diatomite 4-10 part, honey 1-3 part, sucrose mix juice 2-5 part.Although this agaric culture medium matter has high containing selenium content, nutritious, its effect is not so desirable, and the output being mainly manifested in auricularia auriculajudae is not high or not with polysaccharide content.Therefore, it is very valuable for continuing Exploration & stu dy agaric culture medium matter.
Summary of the invention
For above-mentioned the deficiencies in the prior art, technical problem to be solved by this invention proposes a kind of agaric culture medium matter.
A kind of agaric culture medium matter, is made up of each component of following weight: 50 ~ 60 parts, ramulus mori bits, nut-shell 30 ~ 40 parts, Pericarppium arachidis hypogaeae 20 ~ 30 parts, auricularia auriculajudae slag 10 ~ 20 parts, oat slag 15 ~ 20 parts, Luohanguo's residue 10 ~ 15 parts, leaf of bamboo slag 20 ~ 30 parts, 2 ~ 4 parts, lime, 1 ~ 3 part, gypsum, additive 10 ~ 15 parts.
Preferably,
A kind of agaric culture medium matter, be made up of each component of following weight: ramulus mori consider to be worth doing 55 parts, nut-shell 35 parts, Pericarppium arachidis hypogaeae 25 parts, auricularia auriculajudae slag 15 parts, oat slag 18 parts, Luohanguo's residue 12 parts, leaf of bamboo slag 25 parts, 3 parts, lime, 2 parts, gypsum, additive 12 parts.
Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 20 ~ 25 parts, oat extracting solution 10 ~ 20 parts, Grosvenor Momordica extracting solution 10 ~ 15 parts, extract solution of bamboo leaves 5 ~ 7 parts, chrysanthemum extract liquid 6 ~ 8 parts, sodium bentonite 1 ~ 3 part, talcum powder 0.3 ~ 0.5 part, 0.1 ~ 0.3 part, magnesium sulfate, 0.2 ~ 0.4 part, calcium sulfate, 0.1 ~ 0.3 part, zinc sulfate, Sodium Selenite 0.01 ~ 0.03 part, potassium primary phosphate 0.2 ~ 0.4 part.
Preferably,
Described additive is made up of each component of following weight: edible tree fungi extracting liquid 22 parts, oat extracting solution 15 parts, Grosvenor Momordica extracting solution 12 parts, extract solution of bamboo leaves 6 parts, chrysanthemum extract liquid 7 parts, sodium bentonite 2 parts, talcum powder 0.4 part, 0.2 part, magnesium sulfate, 0.3 part, calcium sulfate, 0.2 part, zinc sulfate, Sodium Selenite 0.02 part, potassium primary phosphate 0.3 part.
The preparation of described edible tree fungi extracting liquid, comprises the following steps:
S
1, auricularia auriculajudae is cleaned, be ground into powder, and cross 60 ~ 80 mesh sieves; S
2, add the distilled water of 20 times of weight, soak 30 ~ 40min; S
3, to add massfraction be in the solution the prozyme of 0.1 ~ 0.3%, wherein prozyme is cellulase by weight part ratio: proteolytic enzyme=1:1 mixes, and regulates pH5.0 ~ 6.0, enzymolysis 50 ~ 60min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, add 10 times of weight 40 ~ 50% ethanol, in ultrasonic frequency 30 ~ 40kHz, at temperature 50 ~ 60 DEG C, carry out supersound extraction, extract 40 ~ 50min, centrifugal, filter, collect edible tree fungi extracting liquid and auricularia auriculajudae slag;
The preparation of described oat extracting solution, comprises the following steps:
S
1, by oat cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves, and carry out microwave drying: microwave power 30 ~ 40kW, microwave time 10 ~ 15min; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 30 ~ 50min; S
3, to add massfraction be in the solution the prozyme of 0.06 ~ 0.08%, wherein prozyme is by weight part ratio α-amylase: saccharifying enzyme=1:1 mixes, and regulates pH4.0 ~ 5.0, enzymolysis 40 ~ 50min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, enzymolysis solution after sterilizing is placed in ultrasonic frequency is 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects oat extracting solution and oat slag;
The preparation of described Grosvenor Momordica extracting solution, comprises the following steps:
S
1, by Grosvenor Momordica cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and Grosvenor Momordica extracting solution 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 30 ~ 40min, centrifugal, filters, collect Grosvenor Momordica extracting solution 2, and collect Luohanguo's residue; S
6, merge Grosvenor Momordica extracting solution 1,2, obtain Grosvenor Momordica extracting solution;
The preparation of described extract solution of bamboo leaves, comprises the following steps:
S
1, by the leaf of bamboo cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:10 ~ 15 is heated to 90 ~ 100 DEG C, decocts 20 ~ 30min; S
3, to be placed in ultrasonic frequency be 40 ~ 50KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and extract solution of bamboo leaves 1; S
4, in filter residue, add 60 ~ 70% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 20 ~ 30min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, collect extract solution of bamboo leaves 2, and collect leaf of bamboo slag; S
6, merge extract solution of bamboo leaves 1,2, obtain extract solution of bamboo leaves;
The preparation of described chrysanthemum extract liquid, comprises the following steps:
S
1, by chrysanthemum cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:15 is heated to 90 ~ 100 DEG C, decocts 30 ~ 40min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and chrysanthemum extract liquid 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10, soak 10 ~ 20min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, and collects chrysanthemum extract liquid 2; S
6, merge chrysanthemum extract liquid 1,2, obtain chrysanthemum extract liquid;
The granularity of described nut-shell, Pericarppium arachidis hypogaeae, auricularia auriculajudae slag, oat slag, Luohanguo's residue, leaf of bamboo slag is 60 ~ 80 orders; Described sodium bentonite, talcous granularity are 200 ~ 300 orders.
In the present invention, containing chemical compositions such as abundant polysaccharide, adenosine, melanochrome, ergosterol, phospholipid and multivitamins in edible tree fungi extracting liquid, can as the nutritive ingredient of agaric culture medium matter, especially polysaccharide can be used as carbon source; Containing chemical compositions such as rich in protein, unsaturated fatty acids, water-soluble dietary fibres (especially beta-glucan) in oat extracting solution, can as the Carbon and nitrogen sources of agaric culture medium matter; Containing abundant vitamins C and glucoside, fructose, glucose, protein, lipid etc. in Grosvenor Momordica extracting solution, simultaneously also containing multiple inorganic elements, as calcium, magnesium, selenium, nutritive ingredient can not only be provided like this for agaric culture medium matter, also provide inorganic elements, especially selenium element, reduces selenium compound addition, reduces production cost; Chromocor compound in extract solution of bamboo leaves can suppress varied bacteria growing effectively, protection edible fungus, is conducive to the seed output and quality improving auricularia auriculajudae; Containing compositions such as flavonoid compound, polysaccharide, protein, amino acid in chrysanthemum extract liquid, not only play antibacterial, nitrogenous source, carbon source effect, also play the effect of vitimin supplement, trace element.
Compared with prior art, the invention has the beneficial effects as follows:
Present invention achieves raw material to make full use of, both increased the added value of raw material, reduced production cost again, as auricularia auriculajudae slag/edible tree fungi extracting liquid, oat slag/oat extracting solution, Luohanguo's residue/Grosvenor Momordica extracting solution, leaf of bamboo slag/extract solution of bamboo leaves.Joined in culture medium, be not only culture medium and nutritive ingredient, inorganic elements and trace element are provided, also make culture medium have certain antibacterial, effectively suppress varied bacteria growing, protection edible fungus, be conducive to the seed output and quality improving auricularia auriculajudae.The more important thing is, it can improve the polysaccharide content of the auricularia auriculajudae cultivated, and can also turn waste into wealth simultaneously, economize on resources, and reduces toxigenic capacity.In addition, making full use of of auricularia auriculajudae slag, can not only realize auricularia auriculajudae recycle, can also improve the polysaccharide content of the auricularia auriculajudae cultivated.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
A kind of agaric culture medium matter, is made up of each component of following weight: 50 ~ 60 parts, ramulus mori bits, nut-shell 30 ~ 40 parts, Pericarppium arachidis hypogaeae 20 ~ 30 parts, auricularia auriculajudae slag 10 ~ 20 parts, oat slag 15 ~ 20 parts, Luohanguo's residue 10 ~ 15 parts, leaf of bamboo slag 20 ~ 30 parts, 2 ~ 4 parts, lime, 1 ~ 3 part, gypsum, additive 10 ~ 15 parts.
Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 20 ~ 25 parts, oat extracting solution 10 ~ 20 parts, Grosvenor Momordica extracting solution 10 ~ 15 parts, extract solution of bamboo leaves 5 ~ 7 parts, chrysanthemum extract liquid 6 ~ 8 parts, sodium bentonite 1 ~ 3 part, talcum powder 0.3 ~ 0.5 part, 0.1 ~ 0.3 part, magnesium sulfate, 0.2 ~ 0.4 part, calcium sulfate, 0.1 ~ 0.3 part, zinc sulfate, Sodium Selenite 0.01 ~ 0.03 part, potassium primary phosphate 0.2 ~ 0.4 part.
The preparation of described edible tree fungi extracting liquid, comprises the following steps:
S
1, auricularia auriculajudae is cleaned, be ground into powder, and cross 60 ~ 80 mesh sieves; S
2, add the distilled water of 20 times of weight, soak 30 ~ 40min; S
3, to add massfraction be in the solution the prozyme of 0.1 ~ 0.3%, wherein prozyme is cellulase by weight part ratio: proteolytic enzyme=1:1 mixes, and regulates pH5.0 ~ 6.0, enzymolysis 50 ~ 60min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, add 10 times of weight 40 ~ 50% ethanol, in ultrasonic frequency 30 ~ 40kHz, at temperature 50 ~ 60 DEG C, carry out supersound extraction, extract 40 ~ 50min, centrifugal, filter, collect edible tree fungi extracting liquid and auricularia auriculajudae slag;
The preparation of described oat extracting solution, comprises the following steps:
S
1, by oat cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves, and carry out microwave drying: microwave power 30 ~ 40kW, microwave time 10 ~ 15min; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 30 ~ 50min; S
3, to add massfraction be in the solution the prozyme of 0.06 ~ 0.08%, wherein prozyme is by weight part ratio α-amylase: saccharifying enzyme=1:1 mixes, and regulates pH4.0 ~ 5.0, enzymolysis 40 ~ 50min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, enzymolysis solution after sterilizing is placed in ultrasonic frequency is 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects oat extracting solution and oat slag;
The preparation of described Grosvenor Momordica extracting solution, comprises the following steps:
S
1, by Grosvenor Momordica cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and Grosvenor Momordica extracting solution 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 30 ~ 40min, centrifugal, filters, collect Grosvenor Momordica extracting solution 2, and collect Luohanguo's residue; S
6, merge Grosvenor Momordica extracting solution 1,2, obtain Grosvenor Momordica extracting solution;
The preparation of described extract solution of bamboo leaves, comprises the following steps:
S
1, by the leaf of bamboo cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:10 ~ 15 is heated to 90 ~ 100 DEG C, decocts 20 ~ 30min; S
3, to be placed in ultrasonic frequency be 40 ~ 50KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and extract solution of bamboo leaves 1; S
4, in filter residue, add 60 ~ 70% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 20 ~ 30min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, collect extract solution of bamboo leaves 2, and collect leaf of bamboo slag; S
6, merge extract solution of bamboo leaves 1,2, obtain extract solution of bamboo leaves;
The preparation of described chrysanthemum extract liquid, comprises the following steps:
S
1, by chrysanthemum cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:15 is heated to 90 ~ 100 DEG C, decocts 30 ~ 40min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and chrysanthemum extract liquid 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10, soak 10 ~ 20min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, and collects chrysanthemum extract liquid 2; S
6, merge chrysanthemum extract liquid 1,2, obtain chrysanthemum extract liquid;
Embodiment 1
A kind of agaric culture medium matter, be made up of each component of following weight: ramulus mori consider to be worth doing 55 parts, nut-shell 35 parts, Pericarppium arachidis hypogaeae 25 parts, auricularia auriculajudae slag 15 parts, oat slag 18 parts, Luohanguo's residue 12 parts, leaf of bamboo slag 25 parts, 3 parts, lime, 2 parts, gypsum, additive 12 parts.Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 22 parts, oat extracting solution 15 parts, Grosvenor Momordica extracting solution 12 parts, extract solution of bamboo leaves 6 parts, chrysanthemum extract liquid 7 parts, sodium bentonite 2 parts, talcum powder 0.4 part, 0.2 part, magnesium sulfate, 0.3 part, calcium sulfate, 0.2 part, zinc sulfate, Sodium Selenite 0.02 part, potassium primary phosphate 0.3 part.
Embodiment 2
A kind of agaric culture medium matter, be made up of each component of following weight: ramulus mori consider to be worth doing 50 parts, nut-shell 30 parts, Pericarppium arachidis hypogaeae 20 parts, auricularia auriculajudae slag 10 parts, oat slag 15 parts, Luohanguo's residue 10 parts, leaf of bamboo slag 20 parts, 2 parts, lime, 1 part, gypsum, additive 10 parts.Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 20 parts, oat extracting solution 10 parts, Grosvenor Momordica extracting solution 10 parts, extract solution of bamboo leaves 5 parts, chrysanthemum extract liquid 6 parts, sodium bentonite 1 part, talcum powder 0.3 part, 0.1 part, magnesium sulfate, 0.2 part, calcium sulfate, 0.1 part, zinc sulfate, Sodium Selenite 0.01 part, potassium primary phosphate 0.2 part.
Embodiment 3
A kind of agaric culture medium matter, be made up of each component of following weight: ramulus mori consider to be worth doing 60 parts, nut-shell 40 parts, Pericarppium arachidis hypogaeae 30 parts, auricularia auriculajudae slag 20 parts, oat slag 20 parts, Luohanguo's residue 15 parts, leaf of bamboo slag 30 parts, 4 parts, lime, 3 parts, gypsum, additive 15 parts.Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 25 parts, oat extracting solution 20 parts, Grosvenor Momordica extracting solution 15 parts, extract solution of bamboo leaves 7 parts, chrysanthemum extract liquid 8 parts, sodium bentonite 3 parts, talcum powder 0.5 part, 0.3 part, magnesium sulfate, 0.4 part, calcium sulfate, 0.3 part, zinc sulfate, Sodium Selenite 0.03 part, potassium primary phosphate 0.4 part.
The auricularia auriculajudae comparative result that in the agaric culture medium matter of the embodiment of the present invention 1, embodiment 2, embodiment 3 and background technology, the culture medium of prior art is cultivated out is as shown in table 1:
Table 1 auricularia auriculajudae comparative result
In addition, in order to the advantage of agaric culture medium matter of the present invention is described, applicant carried out following test:
Auricularia polysaccharide extracts yield simultaneous test
The auricularia auriculajudae that the culture medium of prior art in the agaric culture medium matter of the embodiment of the present invention 1, embodiment 2, embodiment 3 and background technology is cultivated out is carried out Polyose extraction respectively, and concrete extracting method is as follows:
S
1, auricularia auriculajudae is cleaned, be ground into powder, and cross 60 mesh sieves; S
2, accurately take 100 grams, auricularia auriculajudae powder, add the distilled water of 20 times of weight, soak 30min; S
3, to add massfraction be in the solution the prozyme of 0.3%, wherein prozyme is cellulase by weight part ratio: proteolytic enzyme=1:1 mixes, and regulates pH5.0, enzymolysis 60min at 50 DEG C; S
4, enzymolysis solution is warming up to 90 DEG C and keeps 10min to carry out sterilizing; S
5, add 20 times of weight 40% ethanol, in ultrasonic frequency 30kHz, under temperature 60 C, carry out supersound extraction, extract 40min, filter, collect filtrate; S
6, filtrate rotary evaporation is concentrated, and with Sevag fado time deproteinated to protein eliminates, dialysis, rotary evaporation concentrates, and add 95% ethanol and make alcohol volume fraction reach 70%, in 4 DEG C of refrigerators, alcohol precipitation spends the night; S
7, be placed in 4000 turns/min whizzer on centrifugal 20 minutes, collecting precipitation thing, dry Crude polysaccharides.Adopt phend-sulphuric acid to measure polysaccharide content in Crude polysaccharides, calculate polysaccharide yield, calculation result is as described in Table 2.
Table 2 Auricularia polysaccharide yield
Certainly, just the preferred embodiments of the disclosure is described in detail above, not limits practical range of the present invention with this, and all equivalence changes done according to principle of the present invention, structure and structure, all should be covered by protection scope of the present invention.
Claims (2)
1. an agaric culture medium matter, is characterized in that: be made up of each component of following weight: 50 ~ 60 parts, ramulus mori bits, nut-shell 30 ~ 40 parts, Pericarppium arachidis hypogaeae 20 ~ 30 parts, auricularia auriculajudae slag 10 ~ 20 parts, oat slag 15 ~ 20 parts, Luohanguo's residue 10 ~ 15 parts, leaf of bamboo slag 20 ~ 30 parts, 2 ~ 4 parts, lime, 1 ~ 3 part, gypsum, additive 10 ~ 15 parts; Wherein, described additive is made up of each component of following weight: edible tree fungi extracting liquid 20 ~ 25 parts, oat extracting solution 10 ~ 20 parts, Grosvenor Momordica extracting solution 10 ~ 15 parts, extract solution of bamboo leaves 5 ~ 7 parts, chrysanthemum extract liquid 6 ~ 8 parts, sodium bentonite 1 ~ 3 part, talcum powder 0.3 ~ 0.5 part, 0.1 ~ 0.3 part, magnesium sulfate, 0.2 ~ 0.4 part, calcium sulfate, 0.1 ~ 0.3 part, zinc sulfate, Sodium Selenite 0.01 ~ 0.03 part, potassium primary phosphate 0.2 ~ 0.4 part.
2. agaric culture medium matter according to claim 1, is characterized in that: the preparation of described edible tree fungi extracting liquid, comprises the following steps:
S
1, auricularia auriculajudae is cleaned, be ground into powder, and cross 60 ~ 80 mesh sieves; S
2, add the distilled water of 20 times of weight, soak 30 ~ 40min; S
3, to add massfraction be in the solution the prozyme of 0.1 ~ 0.3%, wherein prozyme is cellulase by weight part ratio: proteolytic enzyme=1:1 mixes, and regulates pH5.0 ~ 6.0, enzymolysis 50 ~ 60min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, add 10 times of weight 40 ~ 50% ethanol, in ultrasonic frequency 30 ~ 40kHz, at temperature 50 ~ 60 DEG C, carry out supersound extraction, extract 40 ~ 50min, centrifugal, filter, collect edible tree fungi extracting liquid and auricularia auriculajudae slag;
The preparation of described oat extracting solution, comprises the following steps:
S
1, by oat cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves, and carry out microwave drying: microwave power 30 ~ 40kW, microwave time 10 ~ 15min; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 30 ~ 50min; S
3, to add massfraction be in the solution the prozyme of 0.06 ~ 0.08%, wherein prozyme is by weight part ratio α-amylase: saccharifying enzyme=1:1 mixes, and regulates pH4.0 ~ 5.0, enzymolysis 40 ~ 50min at 40 ~ 50 DEG C; S
4, enzymolysis solution is warming up to 90 ~ 100 DEG C and keeps 10 ~ 15min to carry out sterilizing; S
5, enzymolysis solution after sterilizing is placed in ultrasonic frequency is 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects oat extracting solution and oat slag;
The preparation of described Grosvenor Momordica extracting solution, comprises the following steps:
S
1, by Grosvenor Momordica cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, add 30 ~ 40 DEG C of warm water that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and Grosvenor Momordica extracting solution 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 40 ~ 50min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 30 ~ 40min, centrifugal, filters, collect Grosvenor Momordica extracting solution 2, and collect Luohanguo's residue; S
6, merge Grosvenor Momordica extracting solution 1,2, obtain Grosvenor Momordica extracting solution;
The preparation of described extract solution of bamboo leaves, comprises the following steps:
S
1, by the leaf of bamboo cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:10 ~ 15 is heated to 90 ~ 100 DEG C, decocts 20 ~ 30min; S
3, to be placed in ultrasonic frequency be 40 ~ 50KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and extract solution of bamboo leaves 1; S
4, in filter residue, add 60 ~ 70% ethanol that solid-liquid ratio is 1:10 ~ 15, soak 20 ~ 30min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, collect extract solution of bamboo leaves 2, and collect leaf of bamboo slag; S
6, merge extract solution of bamboo leaves 1,2, obtain extract solution of bamboo leaves;
The preparation of described chrysanthemum extract liquid, comprises the following steps:
S
1, by chrysanthemum cleaning screening, then pulverize, and cross 60 ~ 80 mesh sieves; S
2, to add solid-liquid ratio be that the water of 1:15 is heated to 90 ~ 100 DEG C, decocts 30 ~ 40min; S
3, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 40 ~ 50 DEG C, extracts 20 ~ 30min, centrifugal, filters, and collects filter residue and chrysanthemum extract liquid 1; S
4, in filter residue, add 30 ~ 40% ethanol that solid-liquid ratio is 1:10, soak 10 ~ 20min; S
5, to be placed in ultrasonic frequency be 30 ~ 40KHz, extracts at ultrasonic temperature 30 ~ 40 DEG C, extracts 20 ~ 40min, centrifugal, filters, and collects chrysanthemum extract liquid 2; S
6, merge chrysanthemum extract liquid 1,2, obtain chrysanthemum extract liquid.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766377A (en) * | 2016-04-19 | 2016-07-20 | 牡丹江师范学院 | Culture method capable of increasing the flavonoid content and varieties of auricularia auricula |
| CN106045615A (en) * | 2016-05-26 | 2016-10-26 | 柳州市金绿生物科技有限公司 | Black fungus culture medium |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351580A (en) * | 2011-10-14 | 2012-02-15 | 福建农林大学 | Method for preparing black fungus nutrient solution by utilizing food processing residues |
| CN103467169A (en) * | 2013-08-12 | 2013-12-25 | 凤台县星展食用菌有限公司 | Black fungus cultivation base material and preparation method thereof |
| CN104193462A (en) * | 2014-06-30 | 2014-12-10 | 桂林丰茂源农业技术开发有限公司 | Agaric culture medium |
| CN104686198A (en) * | 2015-03-12 | 2015-06-10 | 象州县科学技术局 | Method for cultivation of edible tree funguses through mulberry branches |
| CN104892264A (en) * | 2015-06-19 | 2015-09-09 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii fruit residues in cultivation of black fungi |
| CN104909894A (en) * | 2015-06-03 | 2015-09-16 | 广西大学 | Black fungus culture medium and preparation method thereof |
-
2015
- 2015-12-31 CN CN201511023680.XA patent/CN105418291A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351580A (en) * | 2011-10-14 | 2012-02-15 | 福建农林大学 | Method for preparing black fungus nutrient solution by utilizing food processing residues |
| CN103467169A (en) * | 2013-08-12 | 2013-12-25 | 凤台县星展食用菌有限公司 | Black fungus cultivation base material and preparation method thereof |
| CN104193462A (en) * | 2014-06-30 | 2014-12-10 | 桂林丰茂源农业技术开发有限公司 | Agaric culture medium |
| CN104686198A (en) * | 2015-03-12 | 2015-06-10 | 象州县科学技术局 | Method for cultivation of edible tree funguses through mulberry branches |
| CN104909894A (en) * | 2015-06-03 | 2015-09-16 | 广西大学 | Black fungus culture medium and preparation method thereof |
| CN104892264A (en) * | 2015-06-19 | 2015-09-09 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii fruit residues in cultivation of black fungi |
Cited By (15)
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|---|---|---|---|---|
| CN105766377A (en) * | 2016-04-19 | 2016-07-20 | 牡丹江师范学院 | Culture method capable of increasing the flavonoid content and varieties of auricularia auricula |
| CN107434512A (en) * | 2016-05-26 | 2017-12-05 | 钟建军 | Carbon source is the Grifola frondosa culture material of cotton stalk Luohanguo's residue |
| CN106045615A (en) * | 2016-05-26 | 2016-10-26 | 柳州市金绿生物科技有限公司 | Black fungus culture medium |
| CN107434491A (en) * | 2016-05-26 | 2017-12-05 | 莫秋贵 | Application of the Luohanguo's residue grape branch in Pleurotus tuber-regium compost is made |
| CN107434511A (en) * | 2016-05-26 | 2017-12-05 | 钟建军 | Application of the Luohanguo's residue grape branch in black fungus cultivation material is made |
| CN106171514A (en) * | 2016-07-04 | 2016-12-07 | 贵州根生科技农业有限公司 | A kind of mushroom cultivating method |
| CN106083407A (en) * | 2016-07-28 | 2016-11-09 | 柳州创宇科技有限公司 | A kind of agaric culture medium and fungus cultivation method thereof |
| CN108129223A (en) * | 2016-11-30 | 2018-06-08 | 丹阳市大丰收苗木专业合作社 | A kind of self-control soil-ripening agent |
| CN106518479A (en) * | 2016-12-15 | 2017-03-22 | 李玉龙 | Nutrient solution for narcissus planting |
| CN106699407A (en) * | 2017-01-20 | 2017-05-24 | 陆川县新英食用菌专业合作社 | Culture medium capable of improving quality of selenium-enriched black fungus and preparation method of culture medium |
| CN107258329A (en) * | 2017-08-08 | 2017-10-20 | 莫永忠 | The artificial method for planting of black fungus |
| CN107365198A (en) * | 2017-08-08 | 2017-11-21 | 莫永忠 | The mountain planting method of agaric |
| CN107698317A (en) * | 2017-10-20 | 2018-02-16 | 贵州省印江县印兰生态菌业有限公司 | A kind of mushroom culture medium for improving lentinan content and preparation method thereof |
| CN109089723A (en) * | 2018-07-16 | 2018-12-28 | 绩溪县桐坑源家庭农场 | A kind of implantation methods of high-quality black fungus |
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