CN102550285A - Production method for mycelium pellets of edible and medicinal fungi and products of mycelium pellets of edible and medicinal fungi - Google Patents

Production method for mycelium pellets of edible and medicinal fungi and products of mycelium pellets of edible and medicinal fungi Download PDF

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CN102550285A
CN102550285A CN201210004392XA CN201210004392A CN102550285A CN 102550285 A CN102550285 A CN 102550285A CN 201210004392X A CN201210004392X A CN 201210004392XA CN 201210004392 A CN201210004392 A CN 201210004392A CN 102550285 A CN102550285 A CN 102550285A
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edible
juice
medical fungi
mycelium pellet
mycelium
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CN201210004392XA
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Chinese (zh)
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陈颖
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元拓(福州)生物技术有限公司
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Publication of CN102550285A publication Critical patent/CN102550285A/en

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Abstract

A production method for mycelium pellets of edible and medicinal fungi uses fruit and vegetable juice as main materials obtained by liquid fermentation, and fermentation medium with pH5-8 is made of the fruit and vegetable juice 50-300ml/L, granulated sugar 5-20g/L, KH2PO4 1-5g/L, MgSO4X7H2O 1-5g/L, ZnSO4X7H2O 0-0.5g/L and CaCO3 3-7g/L by adding water to meter the volume. The mycelium pellets of edible and medicinal fungi produced by the method and products of the mycelium pellets have the colors of natural fruit and vegetable juices and functional ingredients of edible and medicinal fungi, and the products are nutritious, unique in flavor, bright and attractive, and have the health functions of coloration, fragrance and taste, and have great market potential.

Description

Preparation method of a kind of edible and medical fungi mycelium pellet and products thereof

Technical field

The present invention relates to preparation method of a kind of edible and medical fungi mycelium pellet and products thereof, is raw material with the Juice particularly, prepares method of edible and medical fungi mycelium pellet and products thereof through solution fermentation.

Technical background

Edible mushroom is a kind of delicious flavour, nutritious health food, and its nutritive value is worth with health care and is mainly reflected in several like this aspects, the one, and protein content is high in the edible mushroom, and contains multiple essential amino acid; The 2nd, carbohydrate content is low in the edible mushroom, is a kind of food low in calories; The 3rd, unsaturated fatty acid content is high in the edible mushroom, the often edible urban disease that can prevent the modern times such as high fat of blood well; The 4th, contain the trace element and the multivitamin of multiple needed by human body such as phosphorus, iron in the edible mushroom; The 5th, edible mushroom contains a large amount of fungi polysaccharides, can effectively improve human immunological competence and anti-tumor capacity, and therefore, FAO's proposition 21 century, the most rational diet structure was " meat or fish one a plain mushroom ".

At present, all kinds of health products that at home and abroad are processed into the edible and medical fungi fruit body like various edible and medical fungi beverages, oral liquid, capsule etc., do not have still that to relate to the Juice be raw material, prepare method of edible and medical fungi mycelium pellet and products thereof through solution fermentation.

Summary of the invention

The objective of the invention is with the Juice is raw material, prepares edible and medical fungi mycelium pellet and products thereof through solution fermentation.

The present invention is a kind of preparation method of edible and medical fungi Juice mycelium pellet; Its making step is: 1.1 carry out activation, rejuvenation with the edible and medical fungi conserving species with potato culture; Put the 20-35 ℃ of static cultivation of insulating box 7-15 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described potato culture is: peeling potato 150-250 g, and glucose 15-25 g, agar 15-20 g adds water and is settled to 1L;

1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L adds water and is settled to 1L; Condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm 2Slant strains, rotary shaking table 70-250rpm cultivated 5-10 days, temperature 20-35 ℃;

1.3 the inoculum concentration of bottle one-level kind with 5-20% shaken in production, and 1.2 described shake-flask culture based formulas and condition of culture enlarged culture 3-7 days once more, must be produced and shake a bottle secondary kind set by step;

1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L, CaCO 33-7g/L adds water and is settled to 1L, pH 5-8; Condition of culture is: inoculum concentration 3-15%, warm 20-35 ℃ in jar, tank pressure 0.03-0.1MPa, speed of agitator 50-200 rev/min, throughput 1:0.2-1.0v/v.min (gas volume that feeds in the per minute in the unit's of being meant zymotic fluid), fermented and cultured 3-5 days;

1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh edible and medical fungi Juice mycelium pellet through filtering.

Said Juice mainly is meant one or more the mixing in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.

Described edible and medical fungi mainly is meant a kind of with in glossy ganoderma, rainbow conk, Uricularia polytricha, tremella, mushroom, straw mushroom, mushroom, flat mushroom, Hericium erinaceus, dictyophora phalloidea, tea tree mushroom, beautiful mushroom, Poria cocos or the Xingbao mushroom.

Preparation method of a kind of edible and medical fungi mycelium pellet of the present invention and products thereof; With in the Juices such as must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, Celery Juice one or more be mixed into raw material; Be meant that with described edible and medical fungi with edible and medical fungis such as glossy ganoderma, rainbow conk, Uricularia polytricha, tremella, mushroom, straw mushroom, mushroom, flat mushroom, Hericium erinaceus, dictyophora phalloidea, tea tree mushroom, beautiful mushroom, Poria cocos or Xingbao mushrooms be starting strain; Carry out liquid fermentation, obtain a large amount of edible and medical fungi mycelium pellets; The color that has contained natural fruit and vegetables juice in the mycelium pellet; Product has nutritious; The unique flavor color is attractive in appearance and the characteristics of certain function of health care are arranged; Therefore can carry out compatibility with the edible and medical fungi mycelium pellet as required, process products such as various edible and medical fungi mycelium pellet dry products, beverage, jelly, oral liquid, capsule.

Fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying.

Fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then be with formulation by weight: select for use 2-5% one or more edible and medical fungi mycelium pellet dry product or 10-25% one or more fresh edible and medical fungi mycelium pellets, edible suspending agent 0.1-0.5%, acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10%, add pure water to 100%; Be made into the mycelium pellet suspending beverage; Wherein said zymotic fluid is meant by the zymotic fluid that makes in the step 1.5; Described acid is meant citric acid or malic acid, and edible suspending agent is the pectin suspending agent.

The production technology of mycelium pellet suspending beverage is: one or more fresh edible and medical fungi mycelium pellets of the edible and medical fungi mycelium pellet dry product of one or more of 2-5% or 10-25%, and the pure water that adds 1-3 times of weight boiled 10-15 minute; Add pectin suspending agent 0.1-0.5% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, the agent of honey 0-3% flavour of candy, Juice 0-15%, one or more zymotic fluids 0-10% then; Add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; Beverage bottling while hot, capping, sterilization that allotment is good; Through being product after the assay was approved, described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.

Fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying, and then be: one or more edible and medical fungi mycelium pellet dry products of 2-5%, or one or more fresh edible and medical fungi mycelium pellets of 10-25% with formulation by weight; Jelly powder (can draw glue or konjac glucomannan) 0.3-0.8%; Acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-10%, one or more zymotic fluids 0-10%; Add pure water to 100%; Be made into edible and medical fungi Juice mycelium pellet jelly, described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.

The production technology of mycelium pellet jelly is: with one or more edible and medical fungi mycelium pellet dry products of 2-5%, or one or more fresh edible and medical fungi mycelium pellets of 10-25%, the pure water that adds 1-3 times of weight boiled 10-15 minute; Add jelly powder (can draw glue or konjac glucomannan) 0.3-0.8% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10% add pure water to 100%, stir; Mycelium pellet jelly package, sterilization that allotment is good are product through after the assay was approved.

Fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein; By weight adding acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3% etc., oral liquid bottling, sterilization that allotment is good; Through being edible and medical fungi oral liquid product after the assay was approved, described acid is meant citric acid or malic acid.

Fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; With said concentrate atomized drying, get edible and medical fungi extract dry powder, adopt prior art to process the edible and medical fungi capsule.

Preparation method of a kind of edible and medical fungi mycelium pellet of the present invention and products thereof; The edible and medical fungi mycelium pellet has contained the color of natural fruit and vegetables juice; Product has nutritious, unique flavor, and color is attractive in appearance and the characteristics of certain function of health care are arranged; Product integrates color, smell and taste and alimentary health-care function, and market potential is big.

Embodiment

Following embodiment can make the professional and technical personnel more understand the present invention, and following embodiment is made amendment, and it all is possible adding and replacing, and does not all exceed protection scope of the present invention.

A kind of preparation method of edible and medical fungi Juice mycelium pellet; Its making step is: 1.1 carry out activation, rejuvenation with the edible and medical fungi conserving species with potato culture; Put the 20-35 ℃ of static cultivation of insulating box 7-15 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described potato culture is: peeling potato 150-250 g, and glucose 15-25 g, agar 15-20 g adds water and is settled to 1L;

1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L adds water and is settled to 1L; Condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm 2Slant strains, rotary shaking table 70-250rpm cultivated 5-10 days, temperature 20-35 ℃;

1.3 the inoculum concentration of bottle one-level kind with 5-20% shaken in production, and 1.2 described shake-flask culture based formulas and condition of culture enlarged culture 3-7 days once more, must be produced and shake a bottle secondary kind set by step;

1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L, CaCO 33-7g/L adds water and is settled to 1L, pH 5-8; Condition of culture is: inoculum concentration 3-15%, warm 20-35 ℃ in jar, tank pressure 0.03-0.1MPa, speed of agitator 50-200 rev/min, throughput 1:0.2-1.0v/v.min, fermented and cultured 3-5 days;

1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh edible and medical fungi Juice mycelium pellet through filtering.

Wherein Juice is meant one or more the mixing in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.

Wherein edible and medical fungi is meant a kind of in glossy ganoderma, rainbow conk, Uricularia polytricha, tremella, mushroom, straw mushroom, mushroom, flat mushroom, Hericium erinaceus, dictyophora phalloidea, tea tree mushroom, beautiful mushroom, Poria cocos or the Xingbao mushroom.

Owing to adopt the culture fluid of natural fruit and vegetables juice as edible and medical fungi; Carry out liquid fermentation; The color that has contained natural fruit and vegetables juice like this in the mycelium pellet; Can carry out compatibility with the edible and medical fungi mycelium pellet as required, process products such as edible and medical fungi mycelium pellet dry product, beverage, jelly, oral liquid, capsule, satisfy people's daily need.The preparation method of various edible and medical fungi mycelium pellet goods is:

Make the method for edible and medical fungi mycelium pellet dry product, fresh edible and medical fungi mycelium pellet obtains edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying.

The method of the mycelium pellet suspending beverage that the edible and medical fungi Juice mycelium pellet of making is made: formulation by weight is: select for use 2-5% one or more edible and medical fungi mycelium pellet dry product or 10-25% one or more fresh edible and medical fungi mycelium pellets, edible suspending agent 0.1-0.5%, acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10%, add pure water to 100%; Described zymotic fluid is meant by the zymotic fluid that makes in the step 1.5; Described acid is meant citric acid or malic acid, and edible suspending agent is the pectin suspending agent.

The production technology of mycelium pellet suspending beverage is: one or more fresh edible and medical fungi mycelium pellets of the edible and medical fungi mycelium pellet dry product of one or more of 2-5% or 10-25%, and the pure water that adds 1-3 times of weight boiled 10-15 minute; Add pectin suspending agent 0.1-0.5% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, the agent of honey 0-3% flavour of candy, Juice 0-15%, one or more zymotic fluids 0-10% then; Add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; Beverage bottling while hot, capping, sterilization that allotment is good; Through being product after the assay was approved, described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.

Mycelium pellet jelly, formulation by weight is: one or more edible and medical fungi mycelium pellet dry products of 2-5%, or one or more fresh edible and medical fungi mycelium pellets of 10-25%; Jelly powder (can draw glue or konjac glucomannan) 0.3-0.8%, acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%; Juice 0-10%, one or more zymotic fluids 0-10%; Add pure water to 100%, described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.

The production technology of mycelium pellet jelly is: one or more edible and medical fungi mycelium pellet dry products of 2-5%, or one or more fresh edible and medical fungi mycelium pellets of 10-25%, and the pure water that adds 1-3 times of weight boiled 10-15 minute; Add jelly powder (can draw glue or konjac glucomannan) 0.3-0.8% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10% add pure water to 100%, stir; Mycelium pellet jelly package, sterilization that allotment is good, through being product after the assay was approved, described acid is meant citric acid or malic acid.

The method of making the edible and medical fungi oral liquid is: with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein; By weight adding acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3% etc., oral liquid bottling, sterilization that allotment is good; Through being edible and medical fungi oral liquid product after the assay was approved, described acid is meant citric acid or malic acid.

The method of making the edible and medical fungi oral capsule is: with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; With said concentrate atomized drying, get edible and medical fungi extract dry powder, adopt prior art to process the edible and medical fungi capsule.

Embodiment 1: preparation method of a kind of tremella mycelium pellet and products thereof may further comprise the steps:

1. the preparation of tremella mycelium pellet

1.1 the tremella conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 26 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 200 g, and glucose 15 g, agar 20 g add water and are settled to 1 L, the pH nature;

1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): carrot juice 200 ml, white granulated sugar 5 g/L, KH 2PO 42g, MgSO 47H 2O 2g, ZnSO 47H 2O 0.25g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 200mL/500mL loading amount, insert 1cm 2Slant strains, 120 rev/mins of rotary shaking tables were cultivated 26 ℃ of temperature 7 days;

1.3 production is shaken bottle one-level kind with 10% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 5 days once more, must produce and shake a bottle secondary kind;

1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): carrot juice 200 ml, white granulated sugar 5 g/L, KH 2PO 42g, MgSO 47H 2O 2g, ZnSO 47H 2O 0.25g, CaCO 33g adds water and is settled to volume requiredly, and pH 6.5; Condition of culture is: inoculum concentration 10%, 26 ℃ of jar temperature, tank pressure 0.05MPa, 120 rev/mins of speeds of agitator, throughput 1:05v/v.min (gas volume that feeds in the per minute in the unit's of being meant zymotic fluid), fermented and cultured 5 days;

1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 7 days, karusen obtains tremella zymotic fluid and fresh tremella mycelium pellet through filtering.

A kind of cultivation the in same available glossy ganoderma, rainbow conk, Uricularia polytricha, mushroom, straw mushroom, mushroom, flat mushroom, Hericium erinaceus, dictyophora phalloidea, tea tree mushroom, beautiful mushroom, Poria cocos or the Xingbao mushroom.

2. tremella mycelium pellet product

2.1 tremella mycelium pellet dry product

Fresh tremella mycelium pellet obtains tremella mycelium pellet dry product after heated-air drying or freeze drying or microwave drying.

2.2 tremella mycelium pellet suspending beverage

Prescription: fresh tremella mycelium pellet 20%, suspending agents such as pectin 0.2%, citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5%, carrot juice 5%, tremella zymotic fluid 5% add pure water to 100%.

Production technology: fresh tremella mycelium pellet 20% adds 2 times of pure water and boiled 10-15 minute; Add suspending agents 0.2% such as pectin then, stirring and dissolving continues heating 3-5 minute; Add citric acid 0.05%, malic acid 0.03%, granulated sugar 6%, honey 0.5%, carrot juice 5%, tremella zymotic fluid 5% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of tremella mycelium pellet suspending beverage, capping, sterilization that allotment is good are tremella mycelium pellet suspending beverage finished product through after the assay was approved.

The mixed juice of one or more in available must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice substitutes carrot juice.

2.3 tremella mycelium pellet jelly

Prescription: tremella mycelium pellet dry product 3%, jelly powder 0.5%, citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5%, pear juice 5%, tremella zymotic fluid 5% add pure water to 100%.

Production technology: tremella mycelium pellet dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5%, pear juice 5%, tremella zymotic fluid 5% add pure water to 100%, stir; Tremella mycelium pellet jelly package, sterilization that allotment is good are product through after the assay was approved.

2.4 tremella oral liquid, capsule

With the water of fresh 2 times of weight of tremella mycelium pellet adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.05-0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.

Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5% etc.; Oral liquid bottling, sterilization that allotment is good are tremella oral liquid product through after the assay was approved.

With said concentrate atomized drying, get tremella extract dry powder, adopt prior art to process the tremella capsule.

Embodiment 2: preparation method of a kind of Ganderma lucidum pompon and products thereof may further comprise the steps:

1. the preparation of Ganderma lucidum pompon

1.1 the glossy ganoderma conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 24 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 250, glucose 15, agar 15, add water be settled to volume required, pH nature;

1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): must 150 ml, white granulated sugar 10 g, KH 2PO 42g, MgSO 47H 2O 2g, ZnSO 47H 2O 0.25g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 250mL/500mL loading amount, insert 1cm 2Slant strains, rotary shaking table 120rpm cultivates 7d, 24 ℃ of temperature;

1.3 production is shaken bottle one-level kind with 10% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 5 days once more, must produce and shake a bottle secondary kind;

1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): must 150 ml, white granulated sugar 10 g, KH 2PO 42g, MgSO 47H 2O 2g, ZnSO 47H 2O 0.25g, CaCO 33g adds water and is settled to volume requiredly, and pH 6.5; Condition of culture is: inoculum concentration 10%, 24 ℃ of jar temperature, tank pressure 0.05MPa, speed of agitator 120rpm, throughput 1:05v/v.min, fermented and cultured 5d;

1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 10 days, karusen obtains ganoderma lucidum fermented liquid and fresh Ganderma lucidum pompon through filtering.

2. Ganderma lucidum pompon product

2.1 Ganderma lucidum mycelium pellet dry product

Obtain Ganderma lucidum pompon dry product after fresh Ganoderma lucidum mycelium ball warp heated-air drying or freeze drying or the microwave drying.

2.2 Ganderma lucidum pompon suspending beverage

Prescription: fresh Ganderma lucidum pompon 20%, suspending agents such as pectin 0.1%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1%, must 3%, ganoderma lucidum fermented liquid 6% add pure water to 100%.

Production technology: fresh Ganderma lucidum pompon 20% adds 2 times of pure water and boiled 10-15 minute; Add suspending agent 0.1-0.5% such as pectin then, stirring and dissolving continues heating 3-5 minute; Add citric acid 0.06%, malic acid 0.02%, granulated sugar 5%, honey 1%, must 3%, ganoderma lucidum fermented liquid 6% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of Ganderma lucidum pompon suspending beverage, capping, sterilization that allotment is good are Ganderma lucidum pompon suspending beverage finished product through after the assay was approved.

2.3 the Ganoderma lucidum mycelium cone freezes

Prescription: Ganderma lucidum pompon dry product 3%, jelly powder 0.5%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1%, must 3%, ganoderma lucidum fermented liquid 6% add pure water to 100%.

Production technology: Ganderma lucidum pompon dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 5%, honey 1%, must 3%, ganoderma lucidum fermented liquid 6% add pure water to 100%, stir; The Ganoderma lucidum mycelium cone that allotment is good freezes packing, sterilization, is product through after the assay was approved.

2.4 Ganoderma lucidum oral liquid, capsule

With the water of fresh 2 times of weight of Ganderma lucidum pompon adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.

Said concentrate is boiled, and behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1% etc. with allotment oral liquid bottling, sterilization well, are the Ganoderma lucidum oral liquid product through after the assay was approved.

With said concentrate atomized drying, get lucidum extracting liquid dry powder, adopt prior art to process ganoderma lucidum capsule.

Embodiment 3: preparation method of a kind of mushroom mycelium ball and products thereof may further comprise the steps:

1. the preparation of mushroom mycelium ball

1.1 the mushroom conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 27 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 150, glucose 20, agar 15, add water be settled to volume required, pH nature;

1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): apple juice 150 ml, white granulated sugar 10 g, KH 2PO 41g, MgSO 47H 2O 1g, ZnSO 47H 2O 0.2g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 250mL/500mL loading amount, insert 1cm 2Slant strains, rotary shaking table 120rpm cultivates 5d, 27 ℃ of temperature;

1.3 production is shaken bottle one-level kind with 10% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 5 days once more, must produce and shake a bottle secondary kind;

1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): apple juice 150 ml, white granulated sugar 10 g, KH 2PO 42g, MgSO 47H 2O 2g, ZnSO 47H 2O 0.25g, CaCO 33g adds water and is settled to volume requiredly, and pH 6.5; Condition of culture is: inoculum concentration 10%, 27 ℃ of jar temperature, tank pressure 0.05MPa, speed of agitator 150rpm, throughput 1:05v/v.min, fermented and cultured 5d;

1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 7 days, karusen obtains Lentinus edodes fermented liquid and fresh mushroom mycelium ball through filtering.

2. mushroom mycelium ball product

2.1 mushroom mycelium ball dry product

Obtain mushroom mycelium ball dry product after fresh mushroom mycelium ball warp heated-air drying or freeze drying or the microwave drying.

2.2 mushroom mycelium ball suspending beverage

Prescription: fresh mushroom mycelium ball 15%, suspending agents such as pectin 0.2%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 0.5%, cabbage juice 3%, Lentinus edodes fermented liquid 5% add pure water to 100%.

Production technology: fresh mushroom mycelium ball 15% adds 2 times of pure water and boiled 10-15 minute; Add suspending agent 0.1-0.5% such as pectin then, stirring and dissolving continues heating 3-5 minute; Add citric acid 0.06%, malic acid 0.02%, granulated sugar 5%, honey 0.5%, cabbage juice 3%, Lentinus edodes fermented liquid 5% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of mushroom mycelium ball suspending beverage, capping, sterilization that allotment is good are mushroom mycelium ball suspending beverage finished product through after the assay was approved.

2.3 the mushroom mycelium cone freezes

Prescription: mushroom mycelium ball dry product 3%, jelly powder 0.5%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 0.5%, apple juice 3%, Lentinus edodes fermented liquid 5% add pure water to 100%.

Production technology: mushroom mycelium ball dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 5%, honey 0.5%, apple juice 3%, Lentinus edodes fermented liquid 5% add pure water to 100%, stir; The mushroom mycelium cone that allotment is good freezes packing, sterilization, is product through after the assay was approved.

2.4 mushroom oral liquid, capsule

With the water of fresh 2 times of weight of mushroom mycelium ball adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.

Said concentrate is boiled, and behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1% etc. with allotment oral liquid bottling, sterilization well, are mushroom oral liquid product through after the assay was approved.

With said concentrate atomized drying, get mushroom extract dry powder, adopt prior art to process mushroom capsule.

Claims (10)

1. the preparation method of an edible and medical fungi Juice mycelium pellet; It is characterized in that: its making step is: 1.1 carry out activation, rejuvenation with the edible and medical fungi conserving species with potato culture; Put the 20-35 ℃ of static cultivation of insulating box 7-15 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described potato culture is: peeling potato 150-250 g, and glucose 15-25 g, agar 15-20 g adds water and is settled to 1L;
1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L adds water and is settled to 1L; Condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm 2Slant strains, rotary shaking table 70-250rpm cultivated 5-10 days, temperature 20-35 ℃;
1.3 the inoculum concentration of bottle one-level kind with 5-20% shaken in production, and 1.2 described shake-flask culture based formulas and condition of culture enlarged culture 3-7 days once more, must be produced and shake a bottle secondary kind set by step;
1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula is: Juice 50-300 ml/L, white granulated sugar 5-20 g/L, KH 2PO 41-5g/L, MgSO 47H 2O 1-5g/L, ZnSO 47H 2O 0-0.5g/L, CaCO 33-7g/L adds water and is settled to 1L, pH 5-8; Condition of culture is: inoculum concentration 3-15%, warm 20-35 ℃ in jar, tank pressure 0.03-0.1MPa, speed of agitator 50-200 rev/min, throughput 1:0.2-1.0v/v.min, fermented and cultured 3-5 days;
1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh edible and medical fungi Juice mycelium pellet through filtering.
2. the preparation method of a kind of edible and medical fungi Juice mycelium pellet according to claim 1 is characterized in that: said Juice is meant one or more the mixing in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.
3. the preparation method of a kind of edible and medical fungi Juice mycelium pellet according to claim 1 is characterized in that: described edible and medical fungi is meant a kind of in glossy ganoderma, rainbow conk, Uricularia polytricha, tremella, mushroom, straw mushroom, mushroom, flat mushroom, Hericium erinaceus, dictyophora phalloidea, tea tree mushroom, beautiful mushroom, Poria cocos or the Xingbao mushroom.
4. the edible and medical fungi Juice mycelium pellet made of a preparation method who utilizes the described edible and medical fungi Juice of the claim 1 mycelium pellet method of making edible and medical fungi mycelium pellet dry product, it is characterized in that: fresh edible and medical fungi mycelium pellet obtains edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying.
5. the mycelium pellet suspending beverage made of the edible and medical fungi Juice mycelium pellet made of a preparation method who utilizes the described edible and medical fungi Juice of claim 1 mycelium pellet; It is characterized in that: fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then be with formulation by weight: select for use 2-5% one or more edible and medical fungi mycelium pellet dry product or 10-25% one or more fresh edible and medical fungi mycelium pellets, edible suspending agent 0.1-0.5%, acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10%, add pure water to 100%; Described zymotic fluid is meant by the zymotic fluid that makes in the step 1.5; Described acid is meant citric acid or malic acid, and edible suspending agent is the pectin suspending agent.
One kind processing mycelium pellet suspending beverage as claimed in claim 5 production technology; It is characterized in that: one or more fresh edible and medical fungi mycelium pellets of the edible and medical fungi mycelium pellet dry product of one or more of 2-5% or 10-25%, the pure water that adds 1-3 times of weight boiled 10-15 minute; Add pectin suspending agent 0.1-0.5% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, the agent of honey 0-3% flavour of candy, Juice 0-15%, one or more zymotic fluids 0-10% then; Add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; Beverage bottling while hot, capping, sterilization that allotment is good; Through being product after the assay was approved, described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.
7. the mycelium pellet jelly made of the edible and medical fungi Juice mycelium pellet made of a preparation method who utilizes the described edible and medical fungi Juice of claim 1 mycelium pellet; It is characterized in that: one or more edible and medical fungi mycelium pellet dry products of 2-5%, or one or more fresh edible and medical fungi mycelium pellets of 10-25%, jelly powder 0.3-0.8%; Acid 0.08%-0.15%; White granulated sugar 3-9%, honey 0-3%, Juice 0-10%, one or more zymotic fluids 0-10% add pure water to 100%; Described zymotic fluid is meant that by the zymotic fluid that makes in the step 1.5, described acid is meant citric acid or malic acid.
One kind processing mycelium pellet jelly as claimed in claim 7 production technology; It is characterized in that: production technology is: one or more edible and medical fungi mycelium pellet dry products of 2-5%; Or one or more fresh edible and medical fungi mycelium pellets of 10-25%, the pure water that adds 1-3 times of weight boiled 10-15 minute; Add jelly powder 0.3-0.8% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, one or more zymotic fluids 0-10% add pure water to 100%, stir; Mycelium pellet jelly package, sterilization that allotment is good, through being product after the assay was approved, described acid is meant citric acid or malic acid.
9. the edible and medical fungi Juice mycelium pellet made of a preparation method who utilizes the described edible and medical fungi Juice of the claim 1 mycelium pellet method of making the edible and medical fungi oral liquid; It is characterized in that: fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein; By weight adding acid 0.08%-0.15%, white granulated sugar 3-9%, honey 0-3% etc., oral liquid bottling, sterilization that allotment is good; Through being edible and medical fungi oral liquid product after the assay was approved, described acid is meant citric acid or malic acid.
10. the edible and medical fungi Juice mycelium pellet made of a preparation method who utilizes the described edible and medical fungi Juice of the claim 1 mycelium pellet method of making the edible and medical fungi oral capsule; It is characterized in that: fresh edible and medical fungi mycelium pellet is obtained edible and medical fungi mycelium pellet dry product after heated-air drying or freeze drying or microwave drying; And then with one or more edible and medical fungi mycelium pellet dry products or one or more fresh edible and medical fungi mycelium pellets; The water that adds 2-5 times of weight; Put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract and filter residue through plate compression; The water that this filter residue is added 2-5 times of weight repeats to extract, filter 1-2 time again; Extract, the zymotic fluid of gained in extracted twice liquid and the step 1.5 is merged 40-75 ℃; 0.05-0.1MPa it is 3-10% that cryogenic vacuum is concentrated into soluble solid, gets concentrate; With said concentrate atomized drying, get edible and medical fungi extract dry powder, adopt prior art to process the edible and medical fungi capsule.
CN201210004392XA 2012-01-09 2012-01-09 Production method for mycelium pellets of edible and medicinal fungi and products of mycelium pellets of edible and medicinal fungi CN102550285A (en)

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CN104256819A (en) * 2014-10-09 2015-01-07 哈尔滨艾克尔食品科技有限公司 Making method for straw mushroom oral liquid
CN104823710A (en) * 2015-04-18 2015-08-12 余臣义 Preparation method of hericium erinaceus mycelium powder
CN105639627A (en) * 2015-12-31 2016-06-08 天津天林欣然生物科技有限公司 Phellinus igniarius juice health-care food and preparation method thereof
CN105725175A (en) * 2016-02-16 2016-07-06 顾伟 Nutritional composition for reducing radiation hazards and tumor suffering risk
CN105876033A (en) * 2016-06-21 2016-08-24 福建农林大学 Bamboo fungus-buckwheat tea and preparation method thereof
CN106119123A (en) * 2016-06-27 2016-11-16 合肥福泉现代农业科技有限公司 A kind of white beech mushroom liquid spawn culture medium based on bean curd yellow pulp water and white beech mushroom liquid spawn preparation method
CN106520450A (en) * 2016-12-22 2017-03-22 阜阳市殿兴农业科技有限公司 Iodine-rich strawberry fruit wine and preparing method thereof
CN106578025A (en) * 2016-11-30 2017-04-26 蚌埠市众星蔬果科技专业合作社联合社 Antistaling agent for storing pomegranate at normal temperature and method for preserving fresh pomegranate by using same
CN106578762A (en) * 2016-12-12 2017-04-26 吉林农业大学 Pleurotus citrinopileatus mycelium pellet and pomelo aerated water and making method thereof
CN106720367A (en) * 2016-12-12 2017-05-31 吉林农业大学 A kind of Pleurotus citrinopileatus mycelium pellet pineapple juice drink and preparation method thereof
CN107494735A (en) * 2017-09-20 2017-12-22 福建农林大学 It is a kind of containing resistant starch, Juice and the health care yoghourt of edible mushroom
CN108034593A (en) * 2018-01-31 2018-05-15 北京农学院 A kind of fungi culture medium and its application
CN108323376A (en) * 2018-03-19 2018-07-27 山东省农业科学院农业资源与环境研究所 A kind of Resistant Volvaria volvacea cultivation material and preparation method thereof and application
CN108587924A (en) * 2018-04-20 2018-09-28 湖南农业大学 A kind of Ganoderma Lucidum pompon product
CN108967049A (en) * 2018-07-05 2018-12-11 贵州省农作物品种资源研究所 A kind of Dictyophora rubrovalvata liquid spawn quick-breeding method

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CN104256819A (en) * 2014-10-09 2015-01-07 哈尔滨艾克尔食品科技有限公司 Making method for straw mushroom oral liquid
CN104823710A (en) * 2015-04-18 2015-08-12 余臣义 Preparation method of hericium erinaceus mycelium powder
CN105639627A (en) * 2015-12-31 2016-06-08 天津天林欣然生物科技有限公司 Phellinus igniarius juice health-care food and preparation method thereof
CN105725175A (en) * 2016-02-16 2016-07-06 顾伟 Nutritional composition for reducing radiation hazards and tumor suffering risk
CN105876033A (en) * 2016-06-21 2016-08-24 福建农林大学 Bamboo fungus-buckwheat tea and preparation method thereof
CN106119123A (en) * 2016-06-27 2016-11-16 合肥福泉现代农业科技有限公司 A kind of white beech mushroom liquid spawn culture medium based on bean curd yellow pulp water and white beech mushroom liquid spawn preparation method
CN106578025A (en) * 2016-11-30 2017-04-26 蚌埠市众星蔬果科技专业合作社联合社 Antistaling agent for storing pomegranate at normal temperature and method for preserving fresh pomegranate by using same
CN106578762A (en) * 2016-12-12 2017-04-26 吉林农业大学 Pleurotus citrinopileatus mycelium pellet and pomelo aerated water and making method thereof
CN106720367A (en) * 2016-12-12 2017-05-31 吉林农业大学 A kind of Pleurotus citrinopileatus mycelium pellet pineapple juice drink and preparation method thereof
CN106520450A (en) * 2016-12-22 2017-03-22 阜阳市殿兴农业科技有限公司 Iodine-rich strawberry fruit wine and preparing method thereof
CN107494735A (en) * 2017-09-20 2017-12-22 福建农林大学 It is a kind of containing resistant starch, Juice and the health care yoghourt of edible mushroom
CN108034593A (en) * 2018-01-31 2018-05-15 北京农学院 A kind of fungi culture medium and its application
CN108323376A (en) * 2018-03-19 2018-07-27 山东省农业科学院农业资源与环境研究所 A kind of Resistant Volvaria volvacea cultivation material and preparation method thereof and application
CN108587924A (en) * 2018-04-20 2018-09-28 湖南农业大学 A kind of Ganoderma Lucidum pompon product
CN108967049A (en) * 2018-07-05 2018-12-11 贵州省农作物品种资源研究所 A kind of Dictyophora rubrovalvata liquid spawn quick-breeding method

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Application publication date: 20120711