CN106699407A - Culture medium capable of improving quality of selenium-enriched black fungus and preparation method of culture medium - Google Patents
Culture medium capable of improving quality of selenium-enriched black fungus and preparation method of culture medium Download PDFInfo
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- CN106699407A CN106699407A CN201710051886.6A CN201710051886A CN106699407A CN 106699407 A CN106699407 A CN 106699407A CN 201710051886 A CN201710051886 A CN 201710051886A CN 106699407 A CN106699407 A CN 106699407A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention relates to the technical field of edible fungus culture, in particular to a culture medium capable of improving the quality of selenium-enriched black fungus and a preparation method of the culture medium. A fungus culture medium is improved, namely composition and formulation of a base material are improved to ensure that the growth state of the fungus reaches the best state; meanwhile, through addition of selenium sulphite and selenium ore for cooperative use, the fungus is rich in selenium; in order to increase the nutritional value of the black fungus, fungus nutrients are increased by adding a nutritional additive; the culture medium achieves the purposes of endowing the fungus with rich selenium and rich nutrition and enabling the fungus to grow fast.
Description
【Technical field】
The present invention relates to edible mushroom culture technique field, more particularly to a kind of culture medium for improving black fungus rich in selenium quality and
Its preparation method.
【Background technology】
Selenium is the essential trace elements of the human body, is referred to as that " life has because of the prevention of its uniqueness, treatment and healthcare function
Effect element " or " life five elementses ".Black fungus quality is delicate, the crisp and refreshing mouth of cunning, nutritious, with important edibility,
Wherein, black fungus includes protein rich in the multiple nutritional components that human body needs, fatty, carbohydrate, fiber, also calcium,
The mineral matters such as phosphorus, iron and multivitamin.But wild black fungus Se content is less, in order to supplement enough selenium units to human body
Element, improves body immunity, and the culture of selenium-rich agaric is particularly important, at present, in the incubation of selenium-rich agaric, often
Selenium-rich is reached by additional inorganic selenium, the addition opportunity of selenium is after agaric fructification grows or adds in the medium,
But, because black fungus is grown up comparatively fast, inorganic selenium is added in the medium, selenium is tended not to be enriched to most preferably when agaric is ripe,
And directly spray selenium to agaric surface after fructification grows, although selenium fast enriching can be made, but agaric has little time toward contact
Inorganic selenium is converted into Organic Selenium just maturation, causes agaric surface to be attached with more inorganic selenium, usage amount is improper also to be made
It is exceeded into Se content；And, not disclosing any culture medium also nowadays can improve the nutritive value of agaric, and richness can be reached again
Selenium effect.Therefore, the present invention needs to solve following problem in the incubation of black fungus rich in selenium：(1) selenium-rich culturing base
The nutritive value of black fungus, particularly protein content, amino acid content, iron content, Vc contents, carbohydrate can be improved to contain
Amount；(2) selenium-rich culturing base can solve the problems, such as that Se content is not enough or selenium can be caused exceeded in prior art cultivation, can be right
Selenium is sustained, and accelerates Se accumulation.
【The content of the invention】
In view of the above, it is necessary to provide a kind of culture medium for improving black fungus rich in selenium quality and preparation method thereof, energy
Accelerate inorganic selenium to be enriched with black fungus, produce the black fungus containing abundant organic selenium composition, but also do not result in Se content
Excessively cause selenosis；Simultaneously, moreover it is possible to improve the nutritive value of black fungus, particularly protein content, amino acid content, iron and contain
Amount, Vc contents, carbohydrate content.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of culture medium for improving black fungus rich in selenium quality, including substrate material, nourishing additive agent and trace element solution, institute
The weight ratio for stating substrate material, nourishing additive agent and trace element solution is：46-53:23-27:3-8；
The substrate material is made up of the raw material of following weight portion：
7 parts -14 parts of selenium ore, 50 parts -63 parts of wood chip, 22 parts -34 parts of wheat bran, 16 parts -24 parts of red bayberry twig powder,
14 parts -23 parts of tea twig powder, 4 parts of -7 parts of density are 1.67 × 103kg/m3Quick lime, 3 parts -7 parts of sulfurous acid selenium and 16 parts -
28 parts of chestnut shell；
The nourishing additive agent is made up of the raw material of following weight portion：
22 parts -34 parts of seawood meal, 6 parts -14 parts of anthocyanidin, 9 parts -19 parts of Lotus Leafextract, 8 parts -17 parts of the moon
Ji Huaye extracts, 14 parts -34 parts of oatmeal, 6 parts -15 parts of Semen Nelumbinis extract and 3 parts -8 parts of egg yolk；
The trace element solution is obtained by the soluble-salt composition configuration of trace element；The trace element solution contains
The material of following mass percent：The ferrous ion of 2%-5%, the magnesium ion of 1%-3%, the potassium ion of 2%-5%, 3%-
6% sodium ion, the zinc ion of 1%-3%, the divalent manganese ion of 2%-5%.
Further, the Se content of the selenium ore is on the basis of selenium ore total amount, by weight percentage, the content of selenium
It is 2%-4%.
Further, the wood chip is selected from least one in China fir, cypress and eucalyptus；The red bayberry twig powder is by strawberry tree
Branch processing is obtained；The tea twig powder is obtained by tea tree branch processing, and the tea tree is selected from white hair tea tree, tea oil tree and blue or green money willow tea tree
In at least one；The wood chip, red bayberry twig powder, the granularity of tea twig powder are the mesh of 300 mesh -500.
Further, the extracting method of the Lotus Leafextract is to be mixed with the water of equal quality after the lotus leaf that will be dried is crushed
Heating is closed, 60min-80min is extracted under the water-bath that temperature is 70 DEG C -80 DEG C；Filtered after extraction, by quality such as filtrate use
Percentage by volume be 60% (v/v) -70% (v/v) ethanol solution refluxing extraction 70min-80min, remove condensate liquid, will be remaining
Lower solution is concentrated, and be concentrated to the volume of concentrate for stoste volume 1/2 obtains Lotus Leafextract.
Further, the Chinese rose leaf extract mixes fresh Chinese rose leaf with the water of equal quality, after boiling
Infusion is carried out with small fire, the time of infusion is 3h-4h, and room temperature is cooled to after infusion, then carry out filtering removal filter residue and obtain the moon
Ji Huaye extracts.
Further, the lotus seeds that the Semen Nelumbinis extract will be dried pulverize, and are then placed in the percentage by volume of equal quality
To soak 5d-6d in the yellow rice wine of 53% (v/v) -65% (v/v), filter residue is removed by filtration after immersion, obtains Semen Nelumbinis extract.
Further, the egg yolk is the egg yolk of raw egg.
Further, the culture medium preparation method of described raising black fungus rich in selenium quality comprises the following steps：
(1) substrate material pretreatment：Selenium ore, chestnut shell are crushed respectively, then by the screen cloth of the mesh of 300 mesh -500
Screened, obtained corresponding selenium mineral powder, chestnut shell powder；Selenium mineral powder, wood chip, chestnut shell are weighed by the parts by weight
Fermented under conditions of being 25 DEG C -28 DEG C in temperature after powder, wheat bran, red bayberry twig powder, tea twig powder and quick lime are well mixed, sent out
Ferment process is irregularly sprayed water in fermentation material, it is ensured that the relative humidity of fermentation material is 40%-50%, a length of 40d- during total fermentation
50d；After the completion of fermentation, to the substrate material that addition sulfurous acid selenium solution in fermentation material is pre-processed；
(2) batch mixing sterilizing：Corresponding substrate material, nourishing additive agent and trace element are weighed by the weight ratio, it is fully mixed
Boiling sterilization is carried out after conjunction and obtains culture medium, the boiling temperature of the boiling sterilization is 130 DEG C -140 DEG C, and digestion time is 8h-
10h。
The present invention has the advantages that：
(1) substrate material formula of the invention is：7 parts -14 parts of selenium ore, 50 parts -63 parts of wood chip, 22 parts -34 parts
Wheat bran, 16 parts -24 parts of red bayberry twig powder, 14 parts -23 parts of tea twig powder, 4 parts of -7 parts of density are 1.67 × 103kg/m3Raw stone
Ash, 3 parts -7 parts of sulfurous acid selenium and 16 parts -28 parts of chestnut shell；Wood chip in substrate material from China fir, cypress and eucalyptus extremely
Few one kind；China fir (latin name：Cunninghamia Lanceolata), cypress (latin name：Platycladus
Orientalis (L.) Francoptmxjjkmsc) and eucalyptus (latin name：Eucalyptus) be all it is wooden neither too hard, nor too soft, carefully
Cause, there is fragrance, the strong trees of corrosion resistant power are adapted to be processed into agaric base-material；Containing a large amount of nutritional ingredients needed by human in wheat bran,
Wherein, p-aminobenzoic acid content is highest in plant, can improve the nutritive value of agaric as substrate material using wheat bran；Together
When, addition red bayberry twig powder can make agaric loose, healthy and strong, but will not accelerate the growth of fungus mycelium, can be added using tea twig powder
The growth of fast fungus mycelium, but influence is not resulted on other nutriments, due to having added other wood chip in invention, such as
Wood chip content is excessive in fruit culture medium, can cause agaric nutrient imbalance, is unfavorable for that agaric grows, and is constantly contrasted by inventor and ground
Study carefully and show that optimal proportioning formula is：50 parts -63 parts of wood chip, 16 parts -24 parts of red bayberry twig powder, 14 parts -23 parts of tea twig powder
Tea twig powder with 14 parts -23 parts；In order to play a part of sterilizing, invention also added quick lime；The present invention is also in substrate
Chestnut shell is with the addition of in material, the cellulose and polysaccharide material content of chestnut shell are of a relatively high, cotton seed hulls can be substituted and provided to agaric
Nutrient, and, the chestnut shell also stink without cotton seed hulls, the agaric smell of culture is more preferable.Meanwhile, in order to improve the selenium of black fungus
Content, the present invention makes black fungus enrichment selenium element reach selenium-rich by adding the inorganic selenium solution of sulfurous acid selenium, but due to black
Agaric growth is rapid, and only addition sulfurous acid selenium solution will not make inorganic selenium fast enriching, and inventor has found by research, in culture
7 parts -14 parts of selenium ore is added in base can accelerate enrichment of the black fungus to selenium element, and selenium ore contains micro selenium unit in itself
Element, meanwhile, also a large amount of micropores have adsorption sustained-release to act on, and can accelerate enrichment of the black fungus to selenium in base-material, and inorganic selenium is converted
It is Organic Selenium；
(2) present invention is provided with corresponding nourishing additive agent to improve the nutritive value of black fungus：22 parts -34 parts
Seawood meal, 6 parts -14 parts of anthocyanidin, 9 parts -19 parts of Lotus Leafextract, 8 parts -17 parts of Chinese rose leaf extract, 14 part -34
Part oatmeal, 6 parts -15 parts of Semen Nelumbinis extract and 3 parts -8 parts of egg yolk；In invention, seawood meal is rich in algal polysaccharides, sweet
The trace element such as dew alcohol, amino acid, protein, vitamin and potassium, iron, calcium, phosphorus, iodine, selenium, cobalt is a kind of foster natural agaric
Point；Anthocyanidin is a kind of natural pigment, and the color and luster of agaric can be made more attractive；Lotus Leafextract, Chinese rose leaf extract and lotus
There is abundant iron simultaneously containing abundant vitamin C in seed extract, enrichment of the agaric to vitamin C and iron can be promoted；
Containing abundant amino acid and protein in oatmeal, egg yolk, agaric can be accelerated to amino using oatmeal and protein
Acid and the enrichment of protein.
【Specific embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine by any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
1st, the culture medium prescription of the present embodiment
The culture medium prescription of the present embodiment includes substrate material, nourishing additive agent and trace element solution, wherein, substrate material,
The weight ratio of nourishing additive agent and trace element solution is：46:23:3；
In above-mentioned formula, substrate material is made up of the raw material of following weight portion：7 parts of selenium ore, 50 parts of wood chip, 22 parts
Wheat bran, 16 parts of red bayberry twig powder, 14 parts of tea twig powder, 4 parts of density are 1.67 × 103kg/m3Quick lime, 3 parts of sulfurous acid selenium
Chestnut shell with 16 parts；
Wherein, on the basis of selenium ore total amount, by weight percentage, the content of selenium is 2% to the Se content of selenium ore；
Wherein, above-mentioned wood chip is selected from China fir, wherein, the granularity of wood chip is 300 mesh；Above-mentioned red bayberry twig powder is added by red bayberry branch
Work is obtained, and the granularity of red bayberry twig powder is 300 mesh；Above-mentioned tea twig powder is obtained by tea tree branch processing, and the granularity of tea twig powder is 300 mesh,
Wherein, tea tree is selected from white hair tea tree.
In above-mentioned formula, nourishing additive agent is made up of the raw material of following weight portion：22 parts of seawood meal, 6 parts of anthocyanidin,
9 parts of Lotus Leafextract, 8 parts of Chinese rose leaf extract, 14 parts of oatmeal, 6 parts of Semen Nelumbinis extract and 3 parts of egg yolk
(wherein, egg yolk of the invention is the egg yolk of raw egg)；
Wherein, the extracting method of Lotus Leafextract is the water Hybrid Heating with equal quality after the lotus leaf that will be dried is crushed,
60min is extracted under the water-bath that temperature is 70 DEG C；Filtered after extraction, the percentage by volume by quality such as filtrate use is 60%
(v/v) ethanol solution refluxing extraction 70min, removes condensate liquid, and resting solution is concentrated, and the volume for being concentrated to concentrate is
The 1/2 of stoste volume obtains Lotus Leafextract；
Wherein, Chinese rose leaf extract mixes fresh Chinese rose leaf with the water of equal quality, is entered with small fire after boiling
Row infusion, the time of infusion is 3h, and room temperature is cooled to after infusion, then carries out filtering removal filter residue and obtains Chinese rose leaf extraction
Thing；
Wherein, the lotus seeds that Semen Nelumbinis extract will be dried pulverize, and the percentage by volume for being then placed in equal quality is 53% (v/
V) 5d is soaked in yellow rice wine, filter residue is removed by filtration after immersion, obtain Semen Nelumbinis extract；
In above-mentioned formula, trace element solution is obtained by the soluble-salt composition configuration of trace element；The trace element
Solution contains the material of following mass percent：2% ferrous ion, 1% magnesium ion, 2% potassium ion, 3% sodium from
Son, 1% zinc ion, 2% divalent manganese ion.
2nd, the culture medium preparation method of the present embodiment raising black fungus rich in selenium quality comprises the following steps：
(1) substrate material pretreatment：Selenium ore, wood chip, chestnut shell are crushed respectively, then by the screen cloth of 300 mesh
Screened, obtained corresponding selenium mineral powder, wood dust, chestnut shell powder；Selenium mineral powder, wood chip are weighed by the parts by weight
Sent out under conditions of being 25 DEG C in temperature after powder, chestnut shell powder, wheat bran, red bayberry twig powder, tea twig powder and quick lime are well mixed
Ferment, fermentation process is irregularly sprayed water in fermentation material, it is ensured that the relative humidity of fermentation material is 40%, a length of 40d during total fermentation；Hair
After the completion of ferment, to the substrate material that addition sulfurous acid selenium solution in fermentation material is pre-processed；
(2) batch mixing sterilizing：Corresponding substrate material, nourishing additive agent and trace element are weighed by the weight ratio, it is fully mixed
Boiling sterilization is carried out after conjunction and obtains culture medium, the boiling temperature of the boiling sterilization is 130 DEG C, and digestion time is 8h.
Embodiment 2：
1st, the culture medium prescription of the present embodiment
The culture medium prescription of the present embodiment includes substrate material, nourishing additive agent and trace element solution, wherein, substrate material,
The weight ratio of nourishing additive agent and trace element solution is：53:27:8；
In above-mentioned formula, substrate material is made up of the raw material of following weight portion：14 parts of selenium ore, 63 parts of wood chip, 34 parts
Wheat bran, 24 parts of red bayberry twig powder, 23 parts of tea twig powder, 7 parts of density be 1.67 × 103kg/m3Quick lime, 7 parts of sulfurous acid
Selenium and 28 parts of chestnut shell；
Wherein, on the basis of selenium ore total amount, by weight percentage, the content of selenium is 4% to the Se content of selenium ore；
Wherein, above-mentioned wood chip is selected from the mixing of cypress and eucalyptus, wherein, the granularity of wood chip is 500 mesh；Above-mentioned red bayberry twig powder
It is obtained by the processing of red bayberry branch, the granularity of red bayberry twig powder is 500 mesh；Above-mentioned tea twig powder is obtained by tea tree branch processing, tea twig powder
Granularity is 500 mesh, wherein, tea tree is selected from the mixing of tea oil tree and blue or green money willow tea tree.
In above-mentioned formula, nourishing additive agent is made up of the raw material of following weight portion：34 parts of seawood meal, 14 parts of cyanine
Element, 19 parts of Lotus Leafextract, 17 parts of Chinese rose leaf extract, 34 parts of oatmeal, 15 parts of Semen Nelumbinis extract and 8 parts
Egg yolk (wherein, egg yolk of the invention is the egg yolk of raw egg)；
Wherein, the extracting method of Lotus Leafextract is the water Hybrid Heating with equal quality after the lotus leaf that will be dried is crushed,
80min is extracted under the water-bath that temperature is 80 DEG C；Filtered after extraction, the percentage by volume by quality such as filtrate use is 70%
(v/v) ethanol solution refluxing extraction 80min, removes condensate liquid, and resting solution is concentrated, and the volume for being concentrated to concentrate is
The 1/2 of stoste volume obtains Lotus Leafextract；
Wherein, Chinese rose leaf extract mixes fresh Chinese rose leaf with the water of equal quality, is entered with small fire after boiling
Row infusion, the time of infusion is 4h, and room temperature is cooled to after infusion, then carries out filtering removal filter residue and obtains Chinese rose leaf extraction
Thing；
Wherein, the lotus seeds that Semen Nelumbinis extract will be dried pulverize, and the percentage by volume for being then placed in equal quality is 65% (v/
V) 6d is soaked in yellow rice wine, filter residue is removed by filtration after immersion, obtain Semen Nelumbinis extract；
In above-mentioned formula, trace element solution is obtained by the soluble-salt composition configuration of trace element；The trace element
Solution contains the material of following mass percent：5% ferrous ion, 3% magnesium ion, 5% potassium ion, 6% sodium from
Son, 3% zinc ion, 5% divalent manganese ion.
2nd, the culture medium preparation method of the present embodiment raising black fungus rich in selenium quality comprises the following steps：
(1) substrate material pretreatment：Selenium ore, wood chip, chestnut shell are crushed respectively, then by the screen cloth of 350 mesh
Screened, obtained corresponding selenium mineral powder, wood dust, chestnut shell powder；Selenium mineral powder, wood chip are weighed by the parts by weight
Sent out under conditions of being 26 DEG C in temperature after powder, chestnut shell powder, wheat bran, red bayberry twig powder, tea twig powder and quick lime are well mixed
Ferment, fermentation process is irregularly sprayed water in fermentation material, it is ensured that the relative humidity of fermentation material is 45%, a length of 45d during total fermentation；Hair
After the completion of ferment, to the substrate material that addition sulfurous acid selenium solution in fermentation material is pre-processed；
(2) batch mixing sterilizing：Corresponding substrate material, nourishing additive agent and trace element are weighed by the weight ratio, it is fully mixed
Boiling sterilization is carried out after conjunction and obtains culture medium, the boiling temperature of the boiling sterilization is 135 DEG C, and digestion time is 9h.
Embodiment 3：
1st, the culture medium prescription of the present embodiment
The culture medium prescription of the present embodiment includes substrate material, nourishing additive agent and trace element solution, wherein, substrate material,
The weight ratio of nourishing additive agent and trace element solution is：40:24:5；
In above-mentioned formula, substrate material is made up of the raw material of following weight portion：10 parts of selenium ore, 55 parts of wood chip, 27 parts
Wheat bran, 20 parts of red bayberry twig powder, 20 parts of tea twig powder, 5 parts of density be 1.67 × 103kg/m3Quick lime, 4 parts of sulfurous acid
Selenium and 20 parts of chestnut shell；
Wherein, on the basis of selenium ore total amount, by weight percentage, the content of selenium is 3% to the Se content of selenium ore；
Wherein, above-mentioned wood chip is selected from the mixing of China fir, cypress and eucalyptus, wherein, the granularity of wood chip is 350 mesh；Above-mentioned poplar
Plum twig powder is obtained by the processing of red bayberry branch, and the granularity of red bayberry twig powder is 350 mesh；Above-mentioned tea twig powder is obtained by tea tree branch processing, tea
The granularity of twig powder is 350 mesh, wherein, tea tree is selected from the mixing of white hair tea tree, tea oil tree and blue or green money willow tea tree.
In above-mentioned formula, nourishing additive agent is made up of the raw material of following weight portion：28 parts of seawood meal, 10 parts of cyanine
Element, 14 parts of Lotus Leafextract, 11 parts of Chinese rose leaf extract, 20 parts of oatmeal, 12 parts of Semen Nelumbinis extract and 5 parts
Egg yolk (wherein, egg yolk of the invention is the egg yolk of raw egg)；
Wherein, the extracting method of Lotus Leafextract is the water Hybrid Heating with equal quality after the lotus leaf that will be dried is crushed,
70min is extracted under the water-bath that temperature is 75 DEG C；Filtered after extraction, the percentage by volume by quality such as filtrate use is 65%
(v/v) ethanol solution refluxing extraction 75min, removes condensate liquid, and resting solution is concentrated, and the volume for being concentrated to concentrate is
The 1/2 of stoste volume obtains Lotus Leafextract；
Wherein, Chinese rose leaf extract mixes fresh Chinese rose leaf with the water of equal quality, is entered with small fire after boiling
Row infusion, the time of infusion is 3.5h, and room temperature is cooled to after infusion, then carries out filtering removal filter residue and obtains Chinese rose leaf extraction
Thing；
Wherein, the lotus seeds that Semen Nelumbinis extract will be dried pulverize, and the percentage by volume for being then placed in equal quality is 60% (v/
V) 5.5d is soaked in yellow rice wine, filter residue is removed by filtration after immersion, obtain Semen Nelumbinis extract；
In above-mentioned formula, trace element solution is obtained by the soluble-salt composition configuration of trace element；The trace element
Solution contains the material of following mass percent：4% ferrous ion, 2% magnesium ion, 4% potassium ion, 5% sodium from
Son, 2% zinc ion, 3% divalent manganese ion.
2nd, the culture medium preparation method of the present embodiment raising black fungus rich in selenium quality comprises the following steps：
(1) substrate material pretreatment：Selenium ore, wood chip, chestnut shell are crushed respectively, then by the screen cloth of 400 mesh
Screened, obtained corresponding selenium mineral powder, wood dust, chestnut shell powder；Selenium mineral powder, wood chip are weighed by the parts by weight
Sent out under conditions of being 28 DEG C in temperature after powder, chestnut shell powder, wheat bran, red bayberry twig powder, tea twig powder and quick lime are well mixed
Ferment, fermentation process is irregularly sprayed water in fermentation material, it is ensured that the relative humidity of fermentation material is 50%, a length of 50d during total fermentation；Hair
After the completion of ferment, to the substrate material that addition sulfurous acid selenium solution in fermentation material is pre-processed；
(2) batch mixing sterilizing：Corresponding substrate material, nourishing additive agent and trace element are weighed by the weight ratio, it is fully mixed
Boiling sterilization is carried out after conjunction and obtains culture medium, the boiling temperature of the boiling sterilization is 140 DEG C, and digestion time is 10h.
Control group 1：
The substrate material of this control group is without selenium ore, and other formulas are identical with embodiment 1.
Control group 2：
Without sulfurous acid selenium in the substrate material of this control group, other formulas are identical with embodiment 1.
Control group 3：
Without seawood meal in the substrate material of this control group, other formulas are identical with embodiment 1.
Control group 4：
Without oatmeal in the substrate material of this control group, other formulas are identical with embodiment 1.
Control group 5：
The nourishing additive agent of this control group is without anthocyanidin, and other formulas are identical with embodiment 1.
Control group 6：
The nourishing additive agent of this control group is without Lotus Leafextract, and other formulas are identical with embodiment 1.
Control group 7：
The nourishing additive agent of this control group is without Chinese rose leaf extract, and other formulas are identical with embodiment 1.
Control group 8：
The nourishing additive agent of this control group is without Semen Nelumbinis extract, and other formulas are identical with embodiment 1.
Control group 9：
The nourishing additive agent of this control group is without egg yolk, and other formulas are identical with embodiment 1.
Control group 10：
The culture medium of this control group is without nourishing additive agent, and other formulas are identical with embodiment 1.
Control group 11：
The culture medium of this control group is without trace element solution, and other formulas are identical with embodiment 1.
Control group 12：
The culture medium of this control group is only cotton seed hulls, bagasse, Mai Pi and quick lime.
Checking test：
With the culture medium inoculated black fungus of embodiment 1-3 and control group 1-12, under equal conditions cultivated, taken out at random
Sample determines the iron content after black fungus maturation, carbohydrate content, Se content, protein content, Vc contents and free amine group
Acid content concrete condition is shown in Table 1：
The black fungus quality versus' table of table 1
As seen from the above table, the iron content of embodiment 1-3 is essentially identical with control group 1-4 and control group 6-8, hence it is evident that higher than right
According to 5 and control group 9-12 of group, illustrate that anthocyanidin and egg yolk in nourishing additive agent of the invention can influence agaric iron content；It is real
The carbohydrate content for applying a 1-3 is essentially identical with control group 1-4 and control group 11, hence it is evident that higher than control group 5-10 and control
Group 12, illustrates that nourishing additive agent of the invention can influence black fungus carbohydrate content；The protein content of embodiment 1-3 with it is right
It is essentially identical according to group 1-2 and control group 11, hence it is evident that higher than control group 3-10 and control group 12, the content of wherein control group 3-4 is inclined
It is low, it is close with control group 12, illustrate that the seawood meal in substrate material, oatmeal and nourishing additive agent can influence the albumen of black fungus to contain
Amount, while nourishing additive agent can also influence the protein content of black fungus, but the seawood meal and oatmeal in substrate material to black wood
The protein content influence of ear is bigger；The Vc contents of embodiment 1-3 are essentially identical with control group 1-5, control group 9, control group 11, bright
Aobvious is higher than control group 6-8, control group 10 and control group 12, illustrates Lotus Leafextract, Chinese rose leaf extraction in nutrient solution of the present invention
Thing, Semen Nelumbinis extract can increase the content of Vc in black fungus；It is the free aminoacid content of embodiment 1-3 and control group 1-2, right
According to 11 essentially identical, slightly above control group 3-4 of group, hence it is evident that apparently higher than control group 5-10, illustrate the sea in substrate material of the present invention
Algae powder, oatmeal and nourishing additive agent can improve the content of free amino acid in black fungus, but nourishing additive agent is to free ammonia
The content influence of base acid is bigger；The Se content of control group 1-3 is essentially identical with control group 3-11, hence it is evident that higher than control group 1-2 and
Control group 12, it was demonstrated that sulfurous acid selenium can improve the Se content in black fungus, selenium ore can accelerate selenium and is enriched with black fungus body.
In sum, can be reached using selenium-rich agaric culture medium of the invention makes agaric selenium-rich, fast-growth while can also
Make the purpose that agaric is nutritious.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of culture medium for improving black fungus rich in selenium quality, including substrate material, nourishing additive agent and trace element solution, it is special
Levy and be, the weight ratio of the substrate material, nourishing additive agent and trace element solution is：46-53:23-27:3-8；
The substrate material is made up of the raw material of following weight portion：
7 parts -14 parts of selenium ore, 50 parts -63 parts of wood chip, 22 parts -34 parts of wheat bran, 16 parts -24 parts of red bayberry twig powder, 14
The tea twig powder of -23 parts of part, 4 parts of -7 parts of density are 1.67 × 103kg/m3Quick lime, 3 parts -7 parts of sulfurous acid selenium and 16 part -28
The chestnut shell of part；
The nourishing additive agent is made up of the raw material of following weight portion：
22 parts -34 parts of seawood meal, 6 parts -14 parts of anthocyanidin, 9 parts -19 parts of Lotus Leafextract, 8 parts -17 parts of China rose
Leaf extract, 14 parts -34 parts of oatmeal, 6 parts -15 parts of Semen Nelumbinis extract and 3 parts -8 parts of egg yolk；
The trace element solution is obtained by the soluble-salt composition configuration of trace element；The trace element solution contains as follows
The material of mass percent：The ferrous ion of 2%-5%, the magnesium ion of 1%-3%, the potassium ion of 2%-5%, 3%-6%
Sodium ion, the zinc ion of 1%-3%, the divalent manganese ion of 2%-5%.
2. it is according to claim 1 it is a kind of improve black fungus rich in selenium quality culture medium, it is characterised in that the selenium ore
Se content on the basis of selenium ore total amount, by weight percentage, the content of selenium is 2%-4%.
3. a kind of culture medium for improving black fungus rich in selenium quality according to claim 1, it is characterised in that wood chip choosing
At least one from China fir, cypress and eucalyptus；The red bayberry twig powder is obtained by the processing of red bayberry branch；The tea twig powder is by tea tree
Branch processing is obtained, and the tea tree is selected from least one in white hair tea tree, tea oil tree and blue or green money willow tea tree；The wood chip, red bayberry
Twig powder, the granularity of tea twig powder are the mesh of 300 mesh -500.
4. it is according to claim 1 it is a kind of improve black fungus rich in selenium quality culture medium, it is characterised in that the lotus leaf is carried
The extracting method for taking thing is the water Hybrid Heating with equal quality after the lotus leaf that will be dried is crushed, and is 70 DEG C -80 DEG C in temperature
60min-80min is extracted under water-bath；Filtered after extraction, by filtrate with etc. quality percentage by volume for 60% (v/v)-
70% (v/v) ethanol solution refluxing extraction 70min-80min, removes condensate liquid, and resting solution is concentrated, and is concentrated to concentration
The volume of liquid obtains Lotus Leafextract for the 1/2 of stoste volume.
5. it is according to claim 1 it is a kind of improve black fungus rich in selenium quality culture medium, it is characterised in that the China rose
Leaf extract mixes fresh Chinese rose leaf with the water of equal quality, carries out infusion after boiling with small fire, and the time of infusion is
3h-4h, is cooled to room temperature after infusion, then carry out filtering removal filter residue and obtain Chinese rose leaf extract.
6. it is according to claim 1 it is a kind of improve black fungus rich in selenium quality culture medium, it is characterised in that the lotus seeds are carried
Take the lotus seeds that thing will dry to pulverize, the percentage by volume for being then placed in equal quality is the yellow rice wine of 53% (v/v) -65% (v/v)
Middle immersion 5d-6d, is removed by filtration filter residue after immersion, obtain Semen Nelumbinis extract.
7. it is according to claim 1 it is a kind of improve black fungus rich in selenium quality culture medium, it is characterised in that the egg yolk
It is the egg yolk of raw egg.
8. according to claim 1-7 any one raising black fungus rich in selenium quality culture medium preparation method, its feature
It is that methods described comprises the following steps：
(1) substrate material pretreatment：Selenium ore, chestnut shell are crushed respectively, is then carried out by the screen cloth of the mesh of 300 mesh -500
Screening, obtains corresponding selenium mineral powder, chestnut shell powder；Selenium mineral powder, wood chip, chestnut shell powder, bran are weighed by the parts by weight
Fermented under conditions of being 25 DEG C -28 DEG C in temperature after skin, red bayberry twig powder, tea twig powder and quick lime are well mixed, fermented
Journey is irregularly sprayed water in fermentation material, it is ensured that the relative humidity of fermentation material is 40%-50%, a length of 40d-50d during total fermentation；Hair
After the completion of ferment, to the substrate material that addition sulfurous acid selenium solution in fermentation material is pre-processed；
(2) batch mixing sterilizing：Corresponding substrate material, nourishing additive agent and trace element are weighed by the weight ratio, after being sufficiently mixed
Carry out boiling sterilization and obtain culture medium, the boiling temperature of the boiling sterilization is 130 DEG C -140 DEG C, and digestion time is 8h-10h.
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| CN111527986A (en) * | 2020-06-08 | 2020-08-14 | 广西壮族自治区林业科学研究院 | Method for planting ganoderma lucidum wood-segment fungus sticks made of camellia oleifera branches in camellia oleifera forest |
| CN113831199A (en) * | 2021-09-29 | 2021-12-24 | 高富嘻(海南)生物科技集团有限公司 | Selenium-rich nutrient solution and preparation method and application thereof |
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