CN106977320A - A kind of culture medium of asparagus and preparation method thereof - Google Patents

A kind of culture medium of asparagus and preparation method thereof Download PDF

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CN106977320A
CN106977320A CN201710361550.XA CN201710361550A CN106977320A CN 106977320 A CN106977320 A CN 106977320A CN 201710361550 A CN201710361550 A CN 201710361550A CN 106977320 A CN106977320 A CN 106977320A
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parts
culture medium
asparagus
cotton seed
megasse
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CN106977320B (en
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陈肇祯
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Tianjin City Baodi District Taifeng And Edible Fungus Ltd
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Tianjin City Baodi District Taifeng And Edible Fungus Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

A kind of culture medium of asparagus and preparation method thereof, in terms of mass fraction, including following raw material:30 50 parts of rice bran;40 60 parts of corncob;48 parts of corn flour;48 parts of dregs of beans;20 32 parts of wheat bran;12 20 parts of soybean skin;8 12 parts of megasse;68 parts of cotton seed hulls;23 parts of Chinese herbal medicine;23 parts of calcium additive;23 parts of orange peel;The culture medium comprehensive nutrition has been reached, antibiotic property has been improved, while the effect increased economic efficiency.

Description

A kind of culture medium of asparagus and preparation method thereof
Technical field
The present invention relates to golden mushroom plantation technical field, the culture matrix of more particularly to a kind of asparagus and its preparation side Method.
Background technology
With 1970s edible mushroom bag stuff cultivation method being widely applied in China, the edible mushroom of each department gives birth to Produce producer and planting household it is more and more, particularly at the beginning of 21 century since, increasingly be particular about health care diet trend under, eat Bacterium industry occurs in that the situation developed rapidly.In common edible fungus culturing species, asparagus is simply easy with its cultivation technique OK, varietal adaptation is strong, can meet different regions plantation needs, and market price is higher, overall economic benefit it is preferably deep by The favor at vast planting edible mushroom family.
Asparagus is the main cultivated edible fungi of China, and asparagus nutrition is extremely enriched, and is a kind of high protein, low in calories, polysaccharide Class health food, containing 18 kinds of amino acid, its arginine, lysine content are higher than general edible mushroom, and teen-age growth is sent out Facilitation is given birth to, the title for having " increasing intelligence mushroom ".In addition asparagus also has higher medical value, and its is cold in nature, can sharp liver, beneficial intestines Stomach, contained Flammulin and asparagus essence has certain preventive and therapeutic effect to cancer, therefore, asparagus be a kind of economic value compared with High edible mushroom.
China is that asparagus is produced and the maximum country of export volume in the world at present, and cultivation asparagus has become mushroom grower and increases income cause Rich important channel.At present, domestic cultivation asparagus raw material is based on cotton seed hulls, because cotton seed hulls raw material resources are deficient so that The price continuous rise of cotton seed hulls, cultivates cost more and more higher, therefore each production unit is all attached great importance to replace to cheap and good-quality For looking for for raw material;In addition Needle mushroom cultivation medium is subject to miscellaneous bacteria infection, influences Growth of Flammulina Velutipes, and low yield causes plantation Family economic benefit is low.To increase economic efficiency, it is antibacterial that planting household uses medicament progress.The asparagus of existing market suppresses miscellaneous bacteria Medicament is generally antibiotic, the problems such as a large amount of uses can cause the raising of the miscellaneous bacteria resistance to the action of a drug, medicament residue, causes the quality institute of asparagus State reduction.
The content of the invention
It is an object of the invention to provide a kind of culture medium of asparagus, the culture medium comprehensive nutrition improves antibiotic property, together When improve economic benefit.
To achieve the above object, the invention provides following technical scheme:A kind of culture medium of asparagus, with mass fraction Meter, including following raw material:30-50 parts of rice bran;40-60 parts of corncob;4-8 parts of corn flour;4-8 parts of dregs of beans;Wheat bran 20-32 Part;12-20 parts of soybean skin;8-12 parts of megasse;6-8 parts of cotton seed hulls;2-3 parts of Chinese herbal medicine;2-3 parts of calcium additive;Orange peel 2-3 parts.
By using above-mentioned technical proposal, in rice bran and wheat bran rich in dietary fiber, crude fat, protein, amino acid, with And vitamin and mineral element etc., provide main nutriment for the growth of asparagus;It is thick containing what is enriched in corncob The content of protein and NFE is far above many straws, and nitrogen source and carbon source are provided for the growth of asparagus;In addition Corncob is more loose, there are many spaces in corncob, and it strengthens the gas permeability of training matrix, and the oxygen of increase culture medium contains Amount so that asparagus preferably grows;This time, corncob is cheap, and more economic values can be brought to raiser;It is beautiful Not only contain thick protein and nitrogen source in ground rice, be the growth of asparagus also containing calcium, irony, vitamin E and trace elements of selenium There is provided necessary nutritional ingredient;It is asparagus synthetic protein and nucleic acid containing abundant protein in soybean skin and dregs of beans Raw material is provided;Cotton seed hulls provides required carbon source for Growth of Flammulina Velutipes, and cotton seed hulls gas permeability preferably, and is respectively provided with certain water lock Function, can keep moisture in culture medium, prevent quick loss;Megasse contains substantial amounts of crude fibre and a small amount of protein, Nutriment is provided for the growth of asparagus, and megasse is first steeped expanded in warm water when in use, so can be with The gas permeability of enhancing training matrix, increases the oxygen content of culture medium so that asparagus preferably grows;Due to the humidity in culture medium Larger, easy breed bacteria suppresses the growth of asparagus, and Chinese herbal medicine can be good at antibacterial action;Calcium additive can be provided The sufficient calcium source of asparagus so that asparagus grows more sturdy, and calcium additive contains calcium carbonate, and calcium carbonate can be adjusted The pH value in culture medium is saved to appropriate value, is conducive to the growth of asparagus.
Preferably, also including peanut shell, the quality of peanut shell is 3-5 times of cotton seed hulls quality.
By using above-mentioned technical proposal, peanut shell gas permeability preferably, and has certain water lock function, Neng Goubao Moisture in culture medium is held, quick loss is prevented;Peanut shell can replace part cotton seed hulls, reduce the consumption of cotton seed hulls, save one Fixed cost of investment, improves economic benefit, contains vitamin B in peanut shell in addition, and asparagus is vitamin B natural defect Type, it is necessary to add vitamin B from the external world, peanut shell disclosure satisfy that demand of the asparagus to vitamin B;And peanut shell and cotton The mass ratio of seed shell is 3-5:Effect preferably, that is, reduces the consumption of cotton seed hulls when 1, have nutrition that culture medium can be kept enough into Point.
Preferably, also including 2-3 parts of seawood meal.
By using above-mentioned technical proposal, containing crude protein, crude fibre and a small amount of polysaccharide in seawood meal, in aqueous It there is-COO- ,-OH ,-NH2Deng group, stable complex compound can be formed with heavy metal ion, so that heavy metal ion With adsorption capacity, prevent heavy metal ion from influenceing the growth of strain;The marine alga held in seawood meal rich in marine organisms is more The trace element such as a variety of marine active compositions such as sugar, mannitol, phycophaein and nitrogen, phosphorus, potassium, iodine, can either improve culture medium Fertility, provides the absorbable nutrient utilized again.
Preferably, it is fresh 5-7 parts also to include peat.
By using above-mentioned technical proposal, peat is fresh to have relatively large hydropote, and the fresh water imbibition ability of peat is stronger, With peanut shell come instead of part cotton seed hulls, due to carrying cotton-wool on cotton seed hulls, its water imbibition is stronger than peanut shell, so peanut shell is replaced Water lock function for the culture medium after cotton seed hulls has declined, and peat is fresh can preferably keep the moisture of culture medium for addition, prevents The only water loss in culture medium;Other peat is fresh to have medical value, there is stronger antibacterial action to fungi.
Preferably, Chinese herb residues include folium isatidis, Cortex Bulbus Allii, honeysuckle, purslane, cactus, ginkgo leaf, China tree, and And its weight ratio is 1:2:1:1:2:2:1.
By using above-mentioned technical proposal, folium isatidis, Cortex Bulbus Allii, honeysuckle, purslane, cactus, ginkgo leaf are respectively provided with The performance of antibacterial, and China tree has pest-resistant effect, and this Chinese herbal medicine property of medicine is weaker, and the harm to asparagus is smaller, and And the use of medicament, the problem of solving medicament residue, it is ensured that the quality of asparagus can be replaced with Chinese herbal medicine.
Preferably, calcium additive includes at least one of bone meal, oyster shell whiting and eggshell.
By using above-mentioned technical proposal, contain natural whiting in bone meal, oyster shell whiting and eggshell, can be in culture medium Increase sufficient calcium source, the growth that asparagus can be promoted more healthy and strong, into the asparagus for growing high calcium, can effectively go out in addition Free acid in matrix, is prevented because the addition of seawood meal and citrus peels makes the growth that is unfavorable for strain acidity is too high;Separately It is at least one that outer eggshell includes egg shell, Ovum Anas domestica putamina and goose egg-shell, in eggshell except containing in substantial amounts of rich in collagen and Hyaluronic acid membrane, can greatly improve the water-retaining property and gas permeability of matrix;The wherein size controlling of eggshell in 0.2-0.25mm, this The eggshell of the particle diameter of sample need not use the substantial amounts of time to go grinding simultaneously and eggshell can be made to have sufficiently large specific surface area, have Beneficial to the growth of strain.
Preferably, orange peel is included in shaddock pericarp, orange pericarp, orange pericarp, lemon pericarp, mandarin orange fruit pericarp extremely Few one kind.
By using above-mentioned technical proposal, multivitamin and organic acid are rich in orange peel, can be carried for strain For nutriment, while also having physiologically active and stimulation, promote the nutrient absorption and growth metabolism of strain, promote root system Growth and resistance against diseases, can effectively improve crop yield and improve product quality;It is fine that thin skin on the inside of pericarp contains meals Peacekeeping pectin, coordinates the seawood meal with water formation colloidality dope, can form colloidality water conservation net inside the base and improve water conservation Property, while making matrix be difficult loosely to be more convenient for using, the string being rich in both it adds the space between water conservation grid, Gas permeability is ensure that while adding moisture-holding capacity.
Another object of the present invention is to provide a kind of preparation method of the culture medium of asparagus, and this method can make Culture medium nutrition is balanced.
To achieve the above object, the invention provides following technical scheme:A kind of preparation method of the culture medium of asparagus, Comprise the following steps:
Step 1, crushing material:Corncob is crushed, 150 mesh sieves are crossed standby;Peanut shell and cotton seed hulls are crushed, 100 mesh sieves are crossed It is standby;Fresh dry of peat is crushed to the particle that particle diameter is 0.2mm-0.3mm;Orange peel is crushed to particle diameter 0.2mm- 0.3mm;
The preparation of step 2, Chinese herbal medicine:By folium isatidis, Radix Isatidis, Cortex Bulbus Allii, honeysuckle, the capsule of weeping forsythia, purslane, cactus, ginkgo Crushed after leaf, China tree mixing, cross 50 mesh sieves standby;
The pretreatment of step 3, megasse:Megasse is steeped in warm water to expanding, taken out standby;
Step 4, material mixing:Material in step 1, step 2 and rice bran, corn flour, dregs of beans, calcium carbonate, soybean skin are put into and stirred Mix and stirred in machine to well mixed;
Step 5, add water stirring:Add water and stir in the mixed material obtained to step 4, water content is reached 80-90%;
Step 6, sterilizing:Culture medium for golden mushroom made from step 5 is sterilized.
By using above-mentioned technical proposal, first dry material is crushed, is so conducive to the well mixed of material;Then prepare Chinese herbal medicine, Chinese herbal medicine is manufactured separately, and by Chinese herbal medicine each component it is well mixed after be then added in material, so can Ensure the integral medicinal energy of Chinese herbal medicine;Other megasse, which is placed on to expand in warm water before the use, first, to be added in culture medium, The gas permeability of training matrix can so be strengthened, increase the oxygen content of culture medium so that asparagus preferably grows;And megasse Pretreatment be located at after prepared by crushing material and Chinese herbal medicine, the megasse expanded can so be entered directly into next In step, the degree diminution for preventing the water loss in megasse placement for a long time, megasse and expanding megasse, influence training Support the gas permeability of base;Added water after material is well mixed, so make the more uniform of material mixing, make culture medium nutrition equal Weighing apparatus;Finally sterilize, the miscellaneous bacteria in culture medium is eliminated, the growth to Flammulina velutipes mycelium provides an excellent growing environment.
Compared with prior art, the characteristics of present invention has following and technique effect:
1st, the present invention instead of part cotton seed hulls using peanut shell, that is, ensure that the nutritional ingredient of culture medium, cottonseed is reduced again The consumption of shell, saves certain cost of investment, increases economic efficiency;
2nd, present invention uses bog moss, with peanut shell come instead of part cotton seed hulls, due to carrying cotton-wool on cotton seed hulls, it absorbs water Property it is stronger than peanut shell, so the water lock function of culture medium that peanut shell substitutes after cotton seed hulls has declined so that the water of culture medium Partial volume is easy to run off, and peat is fresh to have relatively large hydropote, and the fresh water imbibition ability of peat is stronger, and peat is fresh to make up for addition This shortcoming, can preferably keep the moisture of culture medium, prevent the water loss in culture medium;Other peat is fresh with medicinal Value, there is stronger antibacterial action to fungi;
3rd, present invention uses Chinese herbal medicine, this Chinese herbal medicine has the performance of antibacterial, and this Chinese herbal medicine property of medicine is weaker, to asparagus Harm it is smaller, and with Chinese herbal medicine can replace medicament use, the problem of solving medicament residue, it is ensured that asparagus Quality;
4th, present invention uses megasse, megasse contains substantial amounts of crude fibre and a small amount of protein, and megasse is asparagus Growth nutriment is provided, and megasse first expanded when in use, can so strengthen the gas permeability of training matrix, Increase the oxygen content of culture medium so that asparagus preferably grows;
5th, present invention uses seawood meal, containing crude protein, crude fibre and a small amount of polysaccharide in seawood meal, exist in aqueous - COO- ,-OH ,-NH2Deng group, stable complex compound can be formed with heavy metal ion, so that heavy metal ion has Adsorption capacity, prevents heavy metal ion from influenceing the growth of strain.
Embodiment
Involved all substances are commercially available in the embodiment of the present invention.
The cultivation of asparagus, comprises the following steps:
Step 1, the preparation of the culture medium of asparagus
1st, crushing material:Corncob vinasse are crushed, 150 mesh sieves are crossed standby;Peanut shell and cotton seed hulls are crushed, 100 mesh sieves are crossed It is standby;Fresh dry of peat is crushed to the particle that particle diameter is 0.2mm-0.3mm;Orange peel is crushed to particle diameter 0.2mm- 0.3mm;
2nd, the preparation of Chinese herb residues:Folium isatidis, Cortex Bulbus Allii, honeysuckle, purslane, cactus, ginkgo leaf, China tree are mixed laggard Row is crushed, and crosses 50 mesh sieves standby;
3rd, the pretreatment of megasse:Megasse is steeped in warm water to expanding, taken out standby;
4th, material is mixed:Material in step 1, step 2 and step 3 and rice bran, corn flour, dregs of beans, calcium carbonate, soybean skin are put into Stirred in mixer to well mixed;
5th, add water stirring:Add water and stir in the mixed material obtained in 4, water content is reached 80-90%;
6th, sterilize:By obtained culture medium for golden mushroom high-temp steam sterilizing 25 minutes in 5, take out cooling and be divided in blake bottle, It is one group by 16 bottles of blake bottles, is put into same blake bottle frame, then punches:Blake bottle is beaten be through to from top to bottom The inoculating hole that bottom of bottle vertical direction is set, then covers bottle cap.
Step 2, strain is connect
Then the blake bottle that will be equipped with culture medium is moved to sterile workshop, carries out connecing bacterium by connecing bacterium equipment at 18 DEG C, to the greatest extent may be used Equivalent strain can be accessed, and takes into account fast accurate, send out uniform, in time with bottle cap is covered, every bottle of each group connects in interior whole in short-term It is complete.
Step 3, observation and measurement
The culture medium for having connect bacterium of different groups is come in culturing room by sequence number, daily the upgrowth situation of observation mycelia, timing Increment and growing way are recorded, the daily speed of growth is calculated.According to the dense degree of mycelia, mycelia color and the sturdy degree of mycelia, The bacterium infection degree of mycelia is watched simultaneously, and typically in a humid environment, the easy breed bacteria of culture medium causes mycelia to infect Bacterium and fall ill, common are root rot and spot disease, for the ease of distinguish the infected degree of mycelia, be divided into three ranks: "+" is the mycelia of this experimental group not or minimal amount of mycelia obtains root rot and spot disease;" ++ " is the mycelia of this experimental group Root rot and spot disease amount number exceed total hyphae length 1/5 to 1/3;" +++ " for this experimental group mycelia root rot and The amount number of spot disease exceedes the 1/3 of total hyphae length.
The measuring method of mycelium morphology factor:When every bottle of mycelia all starts material feeding and colonized, starting note is made with permanent pen Number, 1 sub-value is surveyed with micrometer separated in time, and average value is calculated, use cmd-1Represent.To record what mycelia was covered with simultaneously Number of days, its data are shown in Table 1- tables 4.
Embodiment 1:The culture medium of asparagus includes rice bran 30Kg;Corncob 40Kg;Corn flour 4Kg;Dregs of beans 4Kg;Wheat bran 20Kg;Soybean skin 12Kg;Megasse 8Kg;Cotton seed hulls 6Kg;Peanut shell 30Kg;Folium isatidis 0.2Kg;Cortex Bulbus Allii 0.4Kg;Honeysuckle 0.2Kg;Purslane 0.2Kg;Cactus 0.4Kg;Ginkgo leaf 0.4Kg;China tree 0.2Kg;Bone meal 1Kg;Oyster shell whiting 0.5Kg;Eggshell Calcium 0.5Kg;Shaddock pericarp 1Kg;Orange pericarp 1Kg;Seawood meal 2Kg;The fresh 5Kg of peat.
Embodiment 2:The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 35Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Gold and silver Flower 0.25Kg;Purslane 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg; Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Embodiment 3:The culture medium of asparagus includes rice bran 50Kg;Corncob 60Kg;Corn flour 8Kg;Dregs of beans 8Kg;Wheat bran 32Kg;Soybean skin 20Kg;Megasse 12Kg;Cotton seed hulls 8Kg;Peanut shell 40Kg;Folium isatidis 0.3Kg;Cortex Bulbus Allii 0.6Kg;Gold and silver Flower 0.3Kg;Purslane 0.3Kg;Cactus 0.6Kg;Ginkgo leaf 0.6Kg;China tree 0.3Kg;Bone meal 1.5Kg;Oyster shell whiting 1.5Kg; Shaddock pericarp 1.5Kg;Orange pericarp 1.5Kg;Seawood meal 3Kg;The fresh 7Kg of peat.
Embodiment 4:The culture medium of asparagus includes rice bran 30Kg;Corncob 40Kg;Corn flour 4Kg;Dregs of beans 4Kg;Wheat bran 20Kg;Soybean skin 12Kg;Megasse 8Kg;Cotton seed hulls 6Kg;Peanut shell 24Kg;Folium isatidis 0.2Kg;Cortex Bulbus Allii 0.4Kg;Honeysuckle 0.2Kg;Purslane 0.2Kg;Cactus 0.4Kg;Ginkgo leaf 0.4Kg;China tree 0.2Kg;Bone meal 1Kg;Oyster shell whiting 0.5Kg;Eggshell Calcium 0.5Kg;Shaddock pericarp 1Kg;Orange pericarp 1Kg;Seawood meal 2Kg;The fresh 5Kg of peat.
Embodiment 5:The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 28Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Gold and silver Flower 0.25Kg;Purslane 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg; Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Embodiment 6:The culture medium of asparagus includes rice bran 50Kg;Corncob 60Kg;Corn flour 8Kg;Dregs of beans 8Kg;Wheat bran 32Kg;Soybean skin 20Kg;Megasse 12Kg;Cotton seed hulls 8Kg;Peanut shell 32Kg;Folium isatidis 0.3Kg;Cortex Bulbus Allii 0.6Kg;Gold and silver Flower 0.3Kg;Purslane 0.3Kg;Cactus 0.6Kg;Ginkgo leaf 0.6Kg;China tree 0.3Kg;Bone meal 1.5Kg;Oyster shell whiting 1.5Kg; Shaddock pericarp 1.5Kg;Orange pericarp 1.5Kg;Seawood meal 3Kg;The fresh 7Kg of peat.
Embodiment 7:The culture medium of asparagus includes rice bran 30Kg;Corncob 40Kg;Corn flour 4Kg;Dregs of beans 4Kg;Wheat bran 20Kg;Soybean skin 12Kg;Megasse 8Kg;Cotton seed hulls 6Kg;Peanut shell 18Kg;Folium isatidis 0.2Kg;Cortex Bulbus Allii 0.4Kg;Honeysuckle 0.2Kg;Purslane 0.2Kg;Cactus 0.4Kg;Ginkgo leaf 0.4Kg;China tree 0.2Kg;Bone meal 1Kg;Oyster shell whiting 0.5Kg;Eggshell Calcium 0.5Kg;Shaddock pericarp 1Kg;Orange pericarp 1Kg;Seawood meal 2Kg;The fresh 5Kg of peat.
Embodiment 8:The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 21Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Gold and silver Flower 0.25Kg;Purslane 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg; Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Embodiment 9:The culture medium of asparagus includes rice bran 50Kg;Corncob 60Kg;Corn flour 8Kg;Dregs of beans 8Kg;Wheat bran 32Kg;Soybean skin 20Kg;Megasse 12Kg;Cotton seed hulls 8Kg;Peanut shell 24Kg;Folium isatidis 0.3Kg;Cortex Bulbus Allii 0.6Kg;Gold and silver Flower 0.3Kg;Purslane 0.3Kg;Cactus 0.6Kg;Ginkgo leaf 0.6Kg;China tree 0.3Kg;Bone meal 1.5Kg;Oyster shell whiting 1.5Kg; Shaddock pericarp 1.5Kg;Orange pericarp 1.5Kg;Seawood meal 3Kg;The fresh 7Kg of peat.
Table 1 is the increment record and speed of growth situation of mycelia in embodiment 1- embodiments 9:From bacterium the 1st day is connect to going out During the existing full bottle of mycelia the 1st, mycelium morphology factor record and the speed of growth
Table 1 is the increment record and speed of growth situation of Flammulina velutipes mycelium in embodiment 1-9
Table 2 is the growing way situation of Flammulina velutipes mycelium
In 1-embodiment of embodiment 3, the mass ratio of peanut shell and cotton seed hulls is 5:1;In 4-embodiment of embodiment 6, peanut shell Mass ratio with cotton seed hulls is 4:1;The mass ratio of peanut shell and cotton seed hulls is 3:1;Reality is can be seen that from the data of Tables 1 and 2 The speed of growth for applying mycelia in 1-embodiment of example 9 is very fast, average out of the 14th day to 29 days daily to be grown in 0.71- 0.732cm·d-1Between, and the cycle grown is shorter, and the growing way of mycelia is preferable:Mycelia is dense, and color is pure white, and stalk is sturdy, And the degree being infected by bacterial is relatively low, particularly embodiment 5, the average daily growth most block out of the 14th day to 29 days is 0.732cm·d-1, and it is 29 days that cycle for growing is most short, so it is sample that the comparative example done below, which is tested with embodiment 5,.
Comparative example then has been done again, has been 6 from the mass ratio of peanut shell and cotton seed hulls in comparative example unlike embodiment 1: 1;The mass ratio of peanut shell and cotton seed hulls is 7 in comparative example 2:1;The mass ratio of peanut shell and cotton seed hulls is 2 in comparative example 3:1; Without addition Chinese herb residues in comparative example 4;It is fresh without addition peat in comparative example 5.
Comparative example 1
The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 42Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Honeysuckle 0.25Kg;Horse Bitterroot 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg;Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Comparative example 2
The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 49Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Honeysuckle 0.25Kg;Horse Bitterroot 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg;Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Comparative example 3
The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 14Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Honeysuckle 0.25Kg;Horse Bitterroot 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg;Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 6Kg of peat.
Comparative example 4
The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 28Kg;Folium isatidis 0Kg;Cortex Bulbus Allii 0Kg;Honeysuckle 0Kg;Purslane 0Kg; Cactus 0Kg;Ginkgo leaf 0Kg;China tree 0Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg;Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Sea Algae powder 2.5Kg;The fresh 6Kg of peat.
Comparative example 5
The culture medium of asparagus includes rice bran 40Kg;Corncob 50Kg;Corn flour 6Kg;Dregs of beans 6Kg;Wheat bran 26Kg;Soybean skin 16Kg;Megasse 10Kg;Cotton seed hulls 7Kg;Peanut shell 28Kg;Folium isatidis 0.25Kg;Cortex Bulbus Allii 0.5Kg;Honeysuckle 0.25Kg;Horse Bitterroot 0.25Kg;Cactus 0.5Kg;Ginkgo leaf 0.5Kg;China tree 0.25Kg;Bone meal 1.5Kg;Oyster shell whiting 1Kg;Shaddock pericarp 1Kg;Orange pericarp 1.5Kg;Seawood meal 2.5Kg;The fresh 0Kg of peat.
Table 3 is the increment record and speed of growth situation of Flammulina velutipes mycelium in comparative example 1-5
Table 4 is the growing way situation of Flammulina velutipes mycelium
The speed of growth, growing way and flower as can be seen that mycelia are contrasted from 1-comparative example of comparative example 3 and 1-embodiment of embodiment 9 Mass values between raw shell and cotton seed hulls have an obvious correlation, the mass ratio with cotton seed hulls of increase peanut shell or reduction The mass ratio with cotton seed hulls of peanut shell, is unfavorable for the speed of growth of mycelia and growing way situation (except the color of mycelia), from It can be seen that the mass ratio with cotton seed hulls of increase peanut shell, the color of mycelia is preferable in table 4;Reduce peanut shell and cotton seed hulls Mass ratio, the color of mycelia is dark yellow;Because predominant amount has crude fibre in peanut shell, nutritive value is relatively low, but it Containing containing vitamin B, and asparagus is vitamin B natural defect type, it is necessary to add vitamin B from the external world, peanut shell can Demand of the asparagus to vitamin B is met, the relatively white light of color of mycelia can be made;And cotton seed hulls contains crude protein, battalion Foster value is higher, when the mass ratio of peanut shell and cotton seed hulls is 3-5:When 1, the rice bran, corn flour, beans of certain content are added The dregs of rice, wheat bran, soybean skin, megasse so that it is more reasonable that the nutritional ingredient of cultural hypha base is distributed so that the growth speed of mycelia Degree, growing way are more preferable.
Contrasted from comparative example 4 with 1-embodiment of embodiment 9, it is fresh without addition peat in comparative example 4;With peanut shell come generation For part cotton seed hulls, due to carrying cotton-wool on cotton seed hulls, its water imbibition is stronger than peanut shell, so peanut shell is substituted after cotton seed hulls The water lock function of culture medium has declined so that the moisture of culture medium is easy to be lost in, and the humidity being caused by culture medium reaches Cause that the speed of mycelial growth is slow less than standard, and mycelia density, stalk is thin and delicate;There is relatively large water suction because peat is fresh Cell, the fresh water imbibition ability of peat is stronger, and peat is fresh can make up this shortcoming for addition, can preferably keep the water of culture medium Point, the water loss in culture medium is prevented, makes to keep preferable humidity environment in culture medium, mycelia is grown up strong and sturdy growth.
Contrasted from comparative example 5 with 1-embodiment of embodiment 9, without addition Chinese herbal medicine in comparative example 5;Culture medium is in itself Humidity is larger, easy breed bacteria so that mycelia is infected by bacterial and fallen ill, and most common mycelia disease is root rot and black spot; The bacterial growth in culture medium can effectively be suppressed by adding Chinese herbal medicine, and this Chinese herbal medicine property of medicine is weaker, to the danger of asparagus Evil is smaller, it is ensured that the quality of asparagus.
In summary, part cotton seed hulls is replaced in this culture medium with peanut shell, the cost of investment can be so reduced, increased Economic benefit, and when the mass ratio of peanut shell and cotton seed hulls is 3-5:When 1, best results;Added simultaneously into culture medium A certain amount of peat is fresh, and peat is fresh to have water lock effect, culture medium is kept preferable humidity environment, mycelia is grown up strong and sturdy; In addition, the addition of Chinese herbal medicine, can effectively suppress the growth of bacterium in culture medium, reduce mycelia and obtained because being infected by bacterial Disease.

Claims (8)

1. a kind of culture medium of asparagus, it is characterised in that in terms of mass fraction, including following raw material:30-50 parts of rice bran; 40-60 parts of corncob;4-8 parts of corn flour;4-8 parts of dregs of beans;20-32 parts of wheat bran;12-20 parts of soybean skin;8-12 parts of megasse;Cotton 6-8 parts of seed shell;2-3 parts of Chinese herbal medicine;2-3 parts of calcium additive;2-3 parts of orange peel.
2. the culture medium of a kind of asparagus according to claim 1, it is characterised in that also including peanut shell, peanut shell Quality is 3-5 times of cotton seed hulls quality.
3. a kind of culture medium of asparagus according to claim 2, it is characterised in that also including 2-3 parts of seawood meal.
4. the culture medium of a kind of asparagus according to claim 3, it is characterised in that also fresh 5-7 parts including peat.
5. a kind of culture medium of asparagus according to claim 1, it is characterised in that the Chinese herbal medicine include folium isatidis, Cortex Bulbus Allii, honeysuckle, purslane, cactus, ginkgo leaf, China tree, and its mass ratio is 1:2:1:1:2:2:1.
6. a kind of culture medium of asparagus according to claim 1, it is characterised in that the calcium additive include bone meal, At least one of shell part, eggshell.
7. the culture medium of a kind of asparagus according to claim 1, it is characterised in that the orange peel includes shaddock fruit At least one of skin, orange pericarp, orange pericarp, lemon pericarp, mandarin orange fruit pericarp.
8. a kind of preparation method for preparing the culture medium such as claim 1-7 any one asparagus, it is characterised in that including with Lower step:
Step 1, crushing material:Corncob is crushed, 150 mesh sieves are crossed standby;Peanut shell and cotton seed hulls are crushed, 100 mesh sieves are crossed It is standby;Fresh dry of peat is crushed to the particle that particle diameter is 0.2mm-0.3mm;Orange peel is crushed to particle diameter 0.2mm- 0.3mm;
The preparation of step 2, Chinese herb residues:Folium isatidis, Cortex Bulbus Allii, honeysuckle, purslane, cactus, ginkgo leaf, China tree are mixed Crushed afterwards, cross 50 mesh sieves standby;
The pretreatment of step 3, megasse:Megasse is steeped in warm water to expanding, taken out standby;
Step 4, material mixing:By material in step 1, step 2 and step 3 and rice bran, corn flour, dregs of beans, calcium carbonate, soybean skin It is put into mixer and stirs to well mixed;
Step 5, add water stirring:Add water and stir in the mixed material obtained to step 4, water content is reached 80-90%;
Step 6, sterilizing:Culture medium for golden mushroom made from step 5 is sterilized.
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107266151A (en) * 2017-08-16 2017-10-20 安徽农耕年华农业发展有限公司 A kind of implantation methods of mushroom
CN107324916A (en) * 2017-08-17 2017-11-07 安徽农耕年华农业发展有限公司 A kind of mushroom plantation culture medium and preparation method
CN107867890A (en) * 2017-11-08 2018-04-03 大新县科学技术情报研究所 A kind of Moringa culture medium and preparation method thereof
CN108046898A (en) * 2017-12-11 2018-05-18 温立超 A kind of culture medium for golden mushroom and preparation method thereof
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CN108887086A (en) * 2018-06-27 2018-11-27 宜昌市慧成知识产权运营有限责任公司 A kind of culture medium for golden mushroom and its proportioner
CN109197377A (en) * 2018-10-26 2019-01-15 濮阳市农业科学院 A kind of cultural method promoting needle mushroom volume increase
CN112568060A (en) * 2020-12-07 2021-03-30 江西省农业生态与资源保护站 Pollution-free culture material and cultivation method for selenium-rich agaricus bisporus
CN112715280A (en) * 2020-12-30 2021-04-30 南京金万辰生物科技有限公司 Flammulina velutipes culture material based on seaweed, preparation method of flammulina velutipes culture material and flammulina velutipes culture method
CN113079949A (en) * 2021-05-11 2021-07-09 广西东兰贵隆生态农业科技有限公司 Edible fungus culture medium and preparation method thereof
CN113099947A (en) * 2021-05-11 2021-07-13 广西东兰贵隆生态农业科技有限公司 Edible fungus culture method
CN114190231A (en) * 2022-01-06 2022-03-18 泗阳华盛生物科技有限公司 Flammulina velutipes culture medium and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104109027A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Oyster mushroom culture medium containing peanut hull and preparation method thereof
CN104151025A (en) * 2014-07-08 2014-11-19 滁州市南谯区大柳镇凤胜食用菌专业合作社 Agaricus blazei murill culture medium taking mulberry branches as raw materials and preparation method of culture medium
CN104829336A (en) * 2015-04-28 2015-08-12 吴中区胥口精益生物医药研究所 Mixed culture base material for pleurotus eryngii culture
CN105036857A (en) * 2015-07-24 2015-11-11 青岛文创科技有限公司 Lentinus edodes culture medium and preparation method thereof
CN106365749A (en) * 2016-09-28 2017-02-01 山东康瑞食用菌科技有限公司 Needle mushroom culture medium and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104151025A (en) * 2014-07-08 2014-11-19 滁州市南谯区大柳镇凤胜食用菌专业合作社 Agaricus blazei murill culture medium taking mulberry branches as raw materials and preparation method of culture medium
CN104109027A (en) * 2014-07-22 2014-10-22 肥东县丰宝种养殖有限责任公司 Oyster mushroom culture medium containing peanut hull and preparation method thereof
CN104829336A (en) * 2015-04-28 2015-08-12 吴中区胥口精益生物医药研究所 Mixed culture base material for pleurotus eryngii culture
CN105036857A (en) * 2015-07-24 2015-11-11 青岛文创科技有限公司 Lentinus edodes culture medium and preparation method thereof
CN106365749A (en) * 2016-09-28 2017-02-01 山东康瑞食用菌科技有限公司 Needle mushroom culture medium and preparation method thereof

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107266151A (en) * 2017-08-16 2017-10-20 安徽农耕年华农业发展有限公司 A kind of implantation methods of mushroom
CN107324916A (en) * 2017-08-17 2017-11-07 安徽农耕年华农业发展有限公司 A kind of mushroom plantation culture medium and preparation method
CN107867890A (en) * 2017-11-08 2018-04-03 大新县科学技术情报研究所 A kind of Moringa culture medium and preparation method thereof
CN108046898A (en) * 2017-12-11 2018-05-18 温立超 A kind of culture medium for golden mushroom and preparation method thereof
CN108834764A (en) * 2018-06-19 2018-11-20 贵州省贵福菌业发展有限公司 A kind of preparation method of oil tea mushroom activated liquid strain
CN108887086A (en) * 2018-06-27 2018-11-27 宜昌市慧成知识产权运营有限责任公司 A kind of culture medium for golden mushroom and its proportioner
CN109197377A (en) * 2018-10-26 2019-01-15 濮阳市农业科学院 A kind of cultural method promoting needle mushroom volume increase
CN112568060A (en) * 2020-12-07 2021-03-30 江西省农业生态与资源保护站 Pollution-free culture material and cultivation method for selenium-rich agaricus bisporus
CN112715280A (en) * 2020-12-30 2021-04-30 南京金万辰生物科技有限公司 Flammulina velutipes culture material based on seaweed, preparation method of flammulina velutipes culture material and flammulina velutipes culture method
CN113079949A (en) * 2021-05-11 2021-07-09 广西东兰贵隆生态农业科技有限公司 Edible fungus culture medium and preparation method thereof
CN113099947A (en) * 2021-05-11 2021-07-13 广西东兰贵隆生态农业科技有限公司 Edible fungus culture method
CN114190231A (en) * 2022-01-06 2022-03-18 泗阳华盛生物科技有限公司 Flammulina velutipes culture medium and preparation method thereof

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