CN108184541A - The production method of Radix Astragali functional edible mushroom - Google Patents
The production method of Radix Astragali functional edible mushroom Download PDFInfo
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- CN108184541A CN108184541A CN201810191837.7A CN201810191837A CN108184541A CN 108184541 A CN108184541 A CN 108184541A CN 201810191837 A CN201810191837 A CN 201810191837A CN 108184541 A CN108184541 A CN 108184541A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of production method of Radix Astragali functional edible mushroom, including：The preparation of Radix Astragali material needed for culture medium, the mother culture media of Radix Astragali functional edible mushroom, liquid spawn culture medium, Cultivar culture medium, management of producing mushroom.It makes full use of rich in the main component in authentic medicinal herbs Radix Astragali, and the Edible Fungi method of all kinds of active component contents in common edible mushroom can be improved, this method is not only able to produce the new type functional edible mushroom rich in the functional activities ingredient such as flavonoids, phenolic acid class, saponins and polysaccharide, and can make full use of local agricultural wastes（Radix Astragali leftover bits and pieces, corncob, cotton seed hulls, sawdust etc.）, and cultural method is easy, growth cycle is short, yield is high, and it is at low cost, easy to spread universal.
Description
Technical field
The present invention relates to edible fungus culturings to plant field, and specially a kind of production method of functional edible mushroom utilizes Huang
Active ingredient in Radix Astragali crude drug and its leftover bits and pieces is enriched with by stilbene functional edible mushroom culture media formula, configuration method step
Novel Radix Astragali functional edible mushroom is cultivated in edible mushroom.
Background technology
Edible fungus culturing production is a wide market, rich people's industry of sustainable development, production raw material master
Local crop by-product resource is utilized, largely discarded Crop transformation can be become by Edible Fungi for people
Edible high-quality protein and healthy food, turn waste into wealth, have do not striven with people grain, do not striven with grain ground, do not striven with ground fertilizer, not with
When agriculture is striven, the features such as taking up an area less, be quick.At present, as the improvement of people's living standards, people to itself health increasingly
Pay attention to, food is increasingly particular about, not only to the nutritional ingredient of food, but also healthcare function value to food etc. is also closed very much
Note.Radix Astragali functional edible mushroom contain it is abundant in addition to the functional components such as original polysaccharide, flavones, saponin(e, phenolic acid greatly improve,
The active constituent being also enriched in Radix Astragali crude drug or leftover bits and pieces, as a kind of novel edible bacterium just meet modern for
The hobby of functional food.
Authentic medicinal herbs of the Radix Astragali as Shanxi Province, the Radix Astragali of Shanxi Province Hunyuan County is either from active component content or medicine
Efficacious prescriptions face is all best for the whole nation, is increased year by year with the demand of Milkvetch Root, the cultivated area of local Radix Astragali
Expanding year by year.Milkvetch Root is mainly used as medicine under ground portion.Radix Astragali crude drug described in the method for the present invention refers to that Radix Astragali harvests
The Radix Astragali main root of Shi Weijing Processing methods；Radix Astragali leftover bits and pieces described in the method for the present invention refers to remove the Huang that may be used as medicinal material
Outside stilbene main root, Radix Astragali stem branch, reed head, lateral root, root can not be specifically included as the Radix Astragali aerial part and under ground portion of medicinal material
And the shorter main root of excellent medicinal material can not be done.Past Radix Astragali main root is used for doing medicinal material, and rest part is then abandoned in Tanaka, agriculture
The people are usually accumulated burning.Great waste is not only caused in this way, and is polluted the environment.
Using Radix Astragali leftover bits and pieces as the matrix of culture edible mushroom, Radix Astragali leftover bits and pieces can either be made full use of, and between energy
What is connect protects environment, it is most important that can also cultivate the functional edible mushroom with more high nutrition and health value.Past
Due to lacking systematic research, by the use of traditional Chinese medicine Radix Astragali leftover bits and pieces as a kind of matrix of culturing edible fungus, additive amount
It is not known but with final edible mushroom yield, the relationship of quality.Artificial add only is instructed by experience, it has been investigated that Radix Astragali
Contain in leftover bits and pieces：32.35 ~ 98.14mg/g of total starches is the 15% ~ 50% of Radix Astragali crude drug；2.62 ~ 4.67mg/g of total phenolics,
It is the 50% ~ 100% of Radix Astragali crude drug；10.24 ~ 17.15mg/g of general flavone is the 50% ~ 100% of Radix Astragali crude drug；Total saposins
10.03 ~ 17.33mg/g is the 50% ~ 90% of Radix Astragali crude drug；Calycosin-7-O-BETA-D-glucoside is with ononin《Chinese Pharmacopoeia》（2015
Version）Described in Radix Astragali main pharmacodynamics ingredient, content of the two in leftover bits and pieces is respectively 13.56 ~ 58.39 μ g/g and 3.78
~ 16.55 μ g/g are the 25% ~ 85% of Radix Astragali crude drug.These data absolutely prove the potentiality of Radix Astragali leftover bits and pieces utilization very
It is huge.
Invention content
Present invention aims at provide a kind of make full use of rich in the main component in authentic medicinal herbs Radix Astragali, and can improve
The Edible Fungi method of all kinds of active component contents in common edible mushroom, this method be not only able to produce rich in flavonoids,
The new type functional edible mushroom of the functional activities ingredient such as phenolic acid class, saponins and polysaccharide, and locality can be made full use of
Agricultural wastes（Radix Astragali leftover bits and pieces, corncob, cotton seed hulls, sawdust etc.）, and cultural method is easy, growth cycle is short, production
Amount is high, at low cost, easy to spread universal.
The present invention adopts the following technical scheme that realization：
A kind of production method of Radix Astragali functional edible mushroom, including：The preparation of Radix Astragali material needed for culture medium, Radix Astragali are functional
The mother culture media of edible mushroom, liquid spawn culture medium, Cultivar culture medium, management of producing mushroom.
The preparation of Radix Astragali material in culture medium：Radix Astragali crude drug is dried, slicer be cut into after 0.5 ~ 2mm medicine materical crude slice be stored in it is dry
Dry shady place is spare.Radix Astragali leftover bits and pieces drying and crushing, it is spare to be stored in dry shady place.
Formula of each raw material of mother culture media based on parts by weight be：150 ~ 200 parts of potato, 25 ~ 30 parts of agar, Portugal
Grape sugar or 2 ~ 3 parts of 20 ~ 25 parts of sucrose, 1.5 ~ 2 parts of magnesium sulfate, 1.5 ~ 2 parts of potassium dihydrogen phosphate, peptone or dusty yeast, Radix Astragali are former
50 ~ 150 parts of 25 ~ 75 parts of medicinal material or Radix Astragali leftover bits and pieces, 1000 parts of water, pH7.0.
Formula of each raw material of liquid spawn culture medium based on parts by weight be：150 ~ 200 parts of potato, brown sugar or sucrose
10 ~ 15 parts, 10 ~ 12 parts of glucose, 1.0 ~ 1.5 parts of magnesium sulfate, 1.5 ~ 2 parts of potassium dihydrogen phosphate, 2 ~ 3 parts of peptone or dusty yeast,
Vitamin B11 ~ 2 part, 50 ~ 200 parts of 25 ~ 100 parts of Radix Astragali crude drug or Radix Astragali leftover bits and pieces, 1000 parts of water, pH7.0.
Formula of each raw material of Cultivar culture medium based on parts by weight be：Under 50 ~ 150 parts of Radix Astragali crude drug or Radix Astragali
600 ~ 1000 parts of 100 ~ 300 parts of heel, corncob or cotton seed hull, thick 150 ~ 350 parts of sawdust, thin 150 ~ 300 parts of sawdust, wheat bran
150 ~ 300 parts, 10 ~ 30 parts of lime, 30 ~ 50 parts of corn flour, water content 60 ~ 65%, pH7.0 ~ 7.5.
It is as follows：
（1）, astragalus dispensing parent species make
Fetch earth 20 ~ 25 parts of 150 ~ 200 parts of beans, 25 ~ 30 parts of agar, glucose or sucrose, 1.5 ~ 2 parts of magnesium sulfate, di(2-ethylhexyl)phosphate respectively
2 ~ 3 parts of 1.5 ~ 2 parts of hydrogen potassium, peptone or dusty yeast, 25 ~ 75 parts of Radix Astragali crude drug or 50 ~ 150 parts of Radix Astragali leftover bits and pieces, water 1000
Part；
Radix Astragali leftover bits and pieces in 90 ~ 100 DEG C or more water is impregnated 2 ~ 4 hours, Radix Astragali leftover bits and pieces is pulled out, be then placed in be cut into it is thin
The potato of piece adds water and heats and boil, filter, agar is added in filtrate, and heating is boiled and is stirred continuously, boils complete to agar
Until being melted into liquid, glucose or sucrose, peptone or dusty yeast, magnesium sulfate, potassium dihydrogen phosphate are then added, is fully stirred
It mixes uniformly, dispenses test tube while hot；Pressure cooker sterilizing, 0.15 ~ 0.18MPa of pressure are put into, temperature is 120 ~ 125 DEG C of heat preservations 30 ~ 60
Minute, after the completion of sterilizing, obtain Radix Astragali mother culture media；It treats that temperature is down to 70 DEG C, mother culture media is removed into pressure cooker, place
Inclined-plane is inoculated with after anhydrous steam in test tube, and test tube is put into superclean bench before inoculation, ultra violet lamp 30 minutes,
Ultraviolet lamp is closed, starts to be inoculated with edible mushroom parent species, the test tube after inoculation is positioned in incubator and cultivates 7 ~ 14 days, cultivation temperature 20
~ 25 DEG C, humidity 40 ~ 80%.
（2）, astragalus dispensing liquid spawn making
Fetch earth 10 ~ 15 parts of 150 ~ 200 parts of beans, brown sugar or sucrose, 10 ~ 12 parts of glucose, 1.0 ~ 1.5 parts of magnesium sulfate, phosphoric acid respectively
1.5 ~ 2 parts of potassium dihydrogen, peptone or 2 ~ 3 parts of dusty yeast, vitamin B11 ~ 2 part, 25 ~ 100 parts of Radix Astragali crude drug or Radix Astragali are got a foothold
Expect 50 ~ 200 parts, 1000 parts of water, pH7.0；
Radix Astragali leftover bits and pieces in 90 ~ 100 DEG C or more water is impregnated 2 ~ 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and cuts
The potato of flakiness, heating are boiled, filtering, and brown sugar or sucrose, glucose, peptone or dusty yeast, dimension life are added in filtrate
Plain B1, magnesium sulfate, potassium dihydrogen phosphate, be sufficiently stirred, be dispensed into triangular flask, be put into pressure cooker sterilizing, pressure 0.15 ~
0.18MPa, temperature keep the temperature 30 ~ 60 minutes for 120 ~ 125 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium；Treat triangle
It is inoculated with after anhydrous steam in bottle, triangular flask is put into superclean bench before inoculation, ultra violet lamp 30 ~ 60 minutes is closed
Ultraviolet lamp starts inoculation step（1）The Radix Astragali edible mushroom parent species of making, the triangular flask after inoculation are put into shaking table with 80 ~ 160
Rev/min culture 7 ~ 10 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%；
The triangular flask liquid spawn made is accessed and further expands culture in fermentation tank, obtains the Huang for being inoculated with cultivating bag
Stilbene formulation liquid seed.
Liquid spawn fermentation tank technological process：Inspection → purge tank → fermentation tank sterilizes → prepares culture substrate → filter of going out
Core, sterile water, inoculation gun barrel → feeding → sterilizing → cooling down → fermentation tank inoculation → culture, observation, detection → connect bacterium bag.
Step：A, cleaning and inspection.Fermentation tank all must be cleaned thoroughly after each or before reusing,
B, filling disinfection, c, tank body, pipeline sterilization, d, filling matrix, e, sterilizing, f, culture medium cooling, g, inoculation, h, fermentation training are boiled
It supports, i, sampling observation, j, liquid spawn inoculation Radix Astragali functional edible mushroom bacterium bag.
（3）, astragalus dispensing cultigen making
50 ~ 150 parts of Radix Astragali crude drug or 100 ~ 300 parts of Radix Astragali leftover bits and pieces, corncob or 600 ~ 1000 parts of cotton seed hull, thick is taken respectively
150 ~ 300 parts of sawdust, 150 ~ 200 parts of thin sawdust, 150 ~ 300 parts of wheat bran, 10 ~ 30 parts of lime, 30 ~ 50 parts of corn flour, water content
60 ~ 65%, pH value 7.0 ~ 7.5；
When making the astragalus dispensing cultigen based on cotton seed hull, first Radix Astragali crude drug or Radix Astragali leftover bits and pieces and sawdust are pressed with water
1:1.5~1:1.7 ratios have been mixed, after being sufficiently humidified so as to it, then by cotton seed hull and water by 1:1.6~1:1.7 ratio has been mixed, then
Wheat bran, lime, corn flour are added in by formula rate again, is covered 2 ~ 5 hours with plastic film after fully mixing thoroughly；It is adjusted again before pack aqueous
Amount is 60 ~ 65%；
When making the astragalus dispensing cultigen based on corncob, first Radix Astragali crude drug or Radix Astragali leftover bits and pieces and sawdust are pressed with water
1:1.5~1:1.7 ratios have been mixed, then the corncob crushed is immersed in 2 ~ 4 hours in 1 ~ 2% limewash, after pulling out again
It adds in wheat bran, lime, corn flour to mix thoroughly, composting 1 ~ 3 hour；Adjustment pH, water content 60 ~ 68% before pack；
The culture medium high pressure sterilization for installing bag is kept for 2 ~ 4 hours at 121 ~ 126 DEG C, alternatively, normal-pressure sterilization will reach 100 in temperature
It is kept for 24 ~ 48 hours after DEG C；
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, in terms of 0.5kg siccatives each cultivating bag inoculation 10 ~ 15mL Radix Astragalis match
Square liquid spawn, wherein astragalus dispensing liquid spawn bacterium ball concentration 60 ~ 100%, 10 ~ 20/milliliter of bacterium ball number；Connect bacterium
Cultivating bag is put into 25 ~ 30 DEG C of culturing room and cultivates 24 ~ 48 hours, moves into 16 ~ 23 DEG C of culturing room and trains after strain field planting
It supports until mycelia covers with bacterium bag.
（4）, Radix Astragali functional edible mushroom cultivation management
The bacterium bag for covering with mycelia is further cultured for 15 ~ 30 days, it is made further to complete physiological maturity, is then moved into mushroom room, into
Row management of producing mushroom, control day temperature are 16 ~ 23 DEG C, and night temperatures are 5 ~ 15 DEG C, humidity 80 ~ 95%, sunshade network control on daytime
Intensity of illumination processed, 10 ~ 30 days long fruiting flower bud, continues culture 7 ~ 15 days, you can picking Radix Astragali functional edible mushroom, entire fruiting training
It is 32 ~ 75 days to support the period.
The cultivation management mode of different Radix Astragali functional edible mushrooms is different, drying also different from, specific to cultivate
Method is identical with common edible mushroom with drying step.
Culture medium prescription of the present invention, configuration method step, have the advantages that cultivating bag pollution rate is low, yield is high, quality is good,
Active constituent particularly in the Radix Astragali high conversion rate in edible mushroom.The Radix Astragali functionality produced using the method for the present invention is eaten
Bacterium and the traditional edible mushroom produced with the culture medium for being not added with Radix Astragali（Control）It compares, active constituent content is obtained for accordingly
It improves.After measured, wherein：Total starches 45.57 ~ 80.26mg/g of content, increases 10.5 ~ 64.8% compared with the control；Total phenolics
0.12 ~ 1.66mg/g of content increases 30.5 ~ 54.7% compared to control；0.68 ~ 3.83mg/g of general flavone content increases compared to control
Add 30.8 ~ 57.5%；1.43 ~ 4.59 mg/g of total saponin content, 43.5 ~ 175.8% are increased compared to control；Element Se content
For 0.15 ~ 0.32 μ g/g, 10.5 ~ 21.6% are increased compared to control.Radix Astragali main pharmacodynamics ingredient Calycosin-7-O-BETA-D-glucoside and rest-harrow
Glycosides content in the Radix Astragali functional edible mushroom of addition Radix Astragali respectively reaches 0.90 ~ 1.48 μ g/g and 0.35 ~ 0.68 μ g/g, and
It is zero to be not added with content in the common edible mushroom of Radix Astragali.
Specific embodiment
Specific embodiments of the present invention are described in detail below.
Embodiment 1
A kind of Radix Astragali functional edible mushroom（Mushroom）Production method, step is as follows：
（1）, make Radix Astragali functionality mushroom parent species
Fetch earth 200 grams of beans respectively, 25 grams of agar, 20 grams of glucose, 2 grams of magnesium sulfate, 2 grams of potassium dihydrogen phosphate, 3 grams of peptone, yellow
80 grams of stilbene leftover bits and pieces, pH7.0.
Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 2 hours, Radix Astragali leftover bits and pieces is pulled out, is then placed in and thinly slices
Potato, add water to 1000mL, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, removes potato
Piece takes filtrate to use, and filtrate is packed into aluminum pot, adds agar, and heating is boiled and is stirred continuously, and prevents agar from gluing the bottom of a pan and liquid
It overflows, boils until agar is melted into liquid completely, then add glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, fill
Divide and stir evenly, dispense test tube while hot.During packing, try not that liquid medium is made to be sticked to test tube mouth, in order to avoid adhere on tampon.Six
A test tube is put into pressure cooker sterilizing, pressure 0.15MPa a bundle, and temperature keeps the temperature 40 minutes for 121 DEG C, after the completion of sterilizing, obtains Huang
Stilbene mother culture media.It treats that kettle temperature is down to 60 DEG C, mother culture media is removed into pressure cooker, inclined-plane is placed, treats anhydrous in test tube
It is inoculated with after steam, test tube is put into superclean bench, ultra violet lamp 30 minutes before inoculation；Ultraviolet lamp is closed, starts to connect
Kind, every test tube is inoculated with the mushroom parent species of 1 piece of a diameter of 0.5cm size, the test tube after inoculation be positioned over culture 7 in incubator ~
14 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%.
（2）, make Radix Astragali functionality mushroom liquid bacterial culture medium
50 grams of Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and is cut into
200 grams of the potato of thin slice, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, is removed potato block, is taken
Filtrate uses, and 10 grams of brown sugar, 12 grams of glucose, 3 grams of peptone, vitamin B will be added in filtrate11 gram, 2.0 grams of magnesium sulfate,
1.5 grams of potassium dihydrogen phosphate, water 1000mL are sufficiently stirred, and are adjusted pH to 7.0, are dispensed into triangular flask, are put into pressure cooker sterilizing, pressure
Power 0.15MPa, temperature keep the temperature 40 minutes for 121 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium.It treats in triangular flask
It being inoculated with after anhydrous steam, triangular flask is put into superclean bench before inoculation, ultra violet lamp 30 minutes closes ultraviolet lamp,
Start to be inoculated with, each 250mL triangular flasks are inoculated with 3 pieces of a diameter of 0.5cm sizes Radix Astragali mushroom parent species, and the triangular flask after inoculation is put into
It is cultivated 12 days with 100 revs/min in shaking table, 25 DEG C of cultivation temperature, humidity 60%.
The triangular flask liquid spawn made is accessed and further expands culture in fermentation tank（Culture substrate is matched in fermentation tank
Side is identical with Radix Astragali functionality mushroom liquid bacterial culture medium prescription）, be available for inoculation cultivating bag astragalus dispensing liquid
Seed.
（3）, make Radix Astragali functionality cultivating champignon kind culture medium
100 grams of Radix Astragali leftover bits and pieces, 800 grams of cotton seed hull, thick 300 grams of sawdust, thin 200 grams of sawdust, 200 grams of wheat bran, lime are taken respectively
30 grams, 30 grams of corn flour, pH value 7.0.
Radix Astragali leftover bits and pieces and sawdust are pressed 1 with water:1.5~1:1.7 ratios have been mixed, after being sufficiently humidified so as to it, then by cotton seed hull
1 is pressed with water:1.6~1:1.7 ratio has been mixed, and is then added in wheat bran, lime, corn flour by formula rate again, is used after fully mixing thoroughly
Plastic film covers 4 hours, and material is allowed to suction moisture.Water content is adjusted before pack again 65% or so, i.e., has water with hand-tight hold between webs
Dripping is advisable.
The culture medium high pressure sterilization for installing bag is kept for 2 hours at 121 DEG C, alternatively, normal-pressure sterilization will reach 100 DEG C in temperature
It is kept for 24 hours afterwards.
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, each cultivating bag（In terms of 0.5kg siccatives）It is yellow to be inoculated with 15mL
Stilbene liquid spawn（It is shown experimentally that, Radix Astragali has certain inhibiting effect for the growth of mushroom mycelium, and inoculum concentration is small to be caused
Mushroom mycelium was grown slowly, and access amount 15mL is best access amount, wherein astragalus dispensing liquid spawn bacterium ball concentration 70%, bacterium ball
15/milliliter of number）.The cultivating bag for connecting bacterium is first put into 28 DEG C of culturing room and cultivates 48 hours, is moved into after strain field planting
It is cultivated in 23 DEG C of culturing room until mycelia covers with bacterium bag.
（4）, fruiting
The bacterium bag for covering with mycelia is further cultured for 15 days, it is made further to complete physiological maturity, is moved into out after mycelia starts annesl
In mushroom house, management of producing mushroom is carried out, control day temperature is 23 DEG C, and night temperatures are 15 DEG C, more than 85% humidity, sunshade on daytime
Network control intensity of illumination, 20 days long fruiting flower bud continue culture 7 days, you can picking Radix Astragali functionality mushroom, entire mushroom producing culture week
Phase is 42 days.
（5）, it is drying
By the fresh mushroom of harvesting, remove mushroom handle base portion impurity, lamella is placed on sieve upward, is shone 2 days under the sun, is treated mushroom body surface face
It when slightly dry, is then displaced into 50 DEG C of baking room, is dried to when mushroom body pinches slightly dough kneading sensation with hand and raises the temperature to
60 DEG C, temperature is kept to obtain Radix Astragali functionality mushroom dry product until mushroom body is dried.
After measured, the present embodiment cultivates to obtain 6 ~ 8cm of mushroom lid diameter of Radix Astragali functionality mushroom；Average fresh mushroom production is
0.85kg/kg siccatives increase by 3.5% compared with ordinary culture medium control yield.Through UV-VIS spectrophotometry and high-efficient liquid phase color
Spectrometry measures, and bioactive ingredients content is compareed with what ordinary culture medium was cultivated in the Radix Astragali functionality mushroom fruiting body cultivated
For mushroom compared to there is different degrees of raising, detailed results are shown in Table 1.
Bioactive ingredients content in 1 Radix Astragali functionality mushroom fruiting body of table
ND is not detected；N/A is not applied to.
Embodiment 2
A kind of Radix Astragali functional edible mushroom（Pleurotus eryngii）Production method, step is as follows：
（1）, make Radix Astragali functionality Pleurotus eryngii parent species
Fetch earth 180 grams of beans respectively, 20 grams of agar, 25 grams of glucose, 2 grams of magnesium sulfate, 3 grams of potassium dihydrogen phosphate, 2 grams of dusty yeast, yellow
50 grams of stilbene crude drug, water 1000mL, pH7.0.
Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 2 hours, Radix Astragali leftover bits and pieces is pulled out, is then placed in and thinly slices
Potato, add water to 1000mL, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, removes potato
Piece takes filtrate to use, and filtrate is packed into aluminum pot, adds agar, and heating is boiled and is stirred continuously, and prevents agar from gluing the bottom of a pan and liquid
It overflows, boils until agar is melted into liquid completely, then add glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, fill
Divide and stir evenly, dispense test tube while hot.During packing, try not that liquid medium is made to be sticked to test tube mouth, be such as stained with, use wet cloth
It cleans, in order to avoid adhere on tampon.Six test tubes are put into pressure cooker sterilizing, pressure 0.15MPa a bundle, and temperature is 121 DEG C of heat preservations 40
Minute, after the completion of sterilizing, obtain Radix Astragali mother culture media.It treats that kettle temperature is down to 60 DEG C, mother culture media is removed into pressure cooker,
Inclined-plane is placed, is inoculated with after anhydrous steam in test tube, test tube is put into superclean bench before inoculation, ultra violet lamp 30 divides
Clock；Ultraviolet lamp is closed, starts to be inoculated with, every test tube is inoculated with the Pleurotus eryngii parent species of 1 piece of a diameter of 0.5cm size, the examination after inoculation
Pipe is positioned in incubator and cultivates 7 ~ 14 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%.
（2）Make Radix Astragali functionality Cultivation Medium of Liquid Fungus Seeds of Pleurotus Eryngii
75 grams of Radix Astragali crude drug in 90 DEG C or more water is impregnated 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and is cut into
200 grams of the potato of thin slice, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, is removed potato block, is taken
Filtrate uses, and 10 grams of brown sugar, 15 grams of sucrose, 3 grams of dusty yeast, vitamin B will be added in filtrate11 gram, 2.0 grams of magnesium sulfate, phosphorus
2.0 grams of acid dihydride potassium, water 1000mL are sufficiently stirred, and are adjusted pH to 7.0, are dispensed into triangular flask, are put into pressure cooker sterilizing, pressure
0.15MPa, temperature keep the temperature 40 minutes for 121 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium, treat nothing in triangular flask
It is inoculated with after vapor, triangular flask is put into superclean bench before inoculation, ultra violet lamp 30 minutes is closed ultraviolet lamp, opened
Begin to be inoculated with, each 250mL triangular flasks connect 3 pieces of a diameter of 0.5cm sizes Radix Astragali Pleurotus eryngii parent species, and the triangular flask after inoculation, which is put into, to be shaken
It is cultivated 10 days with 130 revs/min in bed, 22 DEG C of cultivation temperature, humidity 50%.
The triangular flask liquid spawn made is accessed and further expands culture in fermentation tank（Culture substrate is matched in fermentation tank
Side is identical with Radix Astragali functionality Cultivation Medium of Liquid Fungus Seeds of Pleurotus Eryngii formula）, the Radix Astragali for being available for inoculation cultivating bag is functional
Pleurotus eryngii formulation liquid seed.
（3）, make Radix Astragali functionality planting almond abalone mushroom kind culture medium
125 grams of Radix Astragali crude drug, 600 grams of corncob, thick 200 grams of sawdust, thin 200 grams of sawdust, 300 grams of wheat bran, 30 grams of lime are beautiful
50 grams of rice flour, pH value 7.0.
Radix Astragali crude drug and sawdust are pressed 1 with water:1.5~1:1.7 ratios have been mixed, then the corncob crushed is immersed in
2 ~ 4 hours in 2% limewash add wheat bran, lime, corn flour and mix thoroughly after pulling out, composting 3 hours.It is adjusted before pack
PH, water content 65% have with hand-tight hold between webs under water droplet.
The culture medium high pressure sterilization for installing bag is kept for 4 hours at 125 DEG C, alternatively, normal-pressure sterilization will reach 100 DEG C in temperature
It is kept for 48 hours afterwards.
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, each cultivating bag（0.5kg siccatives）It is inoculated with 10mL liquid bacterias
Kind（Be shown experimentally that, Radix Astragali for Pleurotus eryngii mycelia growth inhibition unobvious, therefore inoculum concentration be 10mL, astragalus dispensing
Liquid spawn bacterium ball concentration 80%, 20/milliliter of bacterium ball number）.The cultivating bag for connecting bacterium is first put into culture in 28 DEG C of culturing room
It 24 hours, moves into 20 DEG C of culturing room and is cultivated until mycelia covers with bacterium bag after strain field planting.
（4）, fruiting
The bacterium bag for covering with mycelia is further cultured for 20 days, it is made further to complete physiological maturity, is then moved into mushroom room, is gone out
Mushroom manages, and control day temperature is 18 DEG C, and night temperatures are 10 DEG C, more than 85% humidity, and daytime controls illumination strong with sunshade net
Degree, 20 days long fruiting flower bud continue culture 10 days, you can picking Radix Astragali functionality Pleurotus eryngii, entire mushroom producing culture period are 50 days.
（5）, it is drying
By the fresh mushroom of harvesting, remove mushroom handle base portion impurity, be cut into the thin slice that thickness is 0.1cm, slice will longitudinal hacking, stem and bacterium
Lid is connected.The fresh mushroom of same day harvesting, same day chip drying obtain Radix Astragali functionality Pleurotus eryngii dry product.
After measured, the present embodiment cultivates to obtain 70-100 grams of single mushroom weight of Radix Astragali functionality Pleurotus eryngii；Average fresh mushroom production
For 0.70kg/kg siccatives, the culture medium yield with being not added with Radix Astragali maintains an equal level.Through spectrophotometry hair and high-efficient liquid phase color
Spectrometry measures, pair that bioactive ingredients content is cultivated with ordinary culture medium in the Radix Astragali functionality Pleurotus eryngii fructification cultivated
According to Pleurotus eryngii compared to there is different degrees of raising, detailed results are shown in Table 2.
Bioactive ingredients content in 2 Radix Astragali functionality Pleurotus eryngii fructification of table
ND is not detected；N/A is not applied to.
Embodiment 3
A kind of Radix Astragali functional edible mushroom（Pleurotus nebrodensis）Production method, step is as follows：
（1）, make Radix Astragali functionality Pleurotus nebrodensis parent species
Fetch earth 200 grams of beans respectively, 20 grams of agar, 20 grams of sucrose, 2 grams of magnesium sulfate, 3 grams of potassium dihydrogen phosphate, 3 grams of dusty yeast, Radix Astragali
150 grams of leftover bits and pieces, water 1000mL, pH7.0.
Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 2 hours, Radix Astragali leftover bits and pieces is pulled out, is then placed in and thinly slices
Potato, add water to 1000mL, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, removes potato
Piece takes filtrate to use, and filtrate is packed into aluminum pot, adds agar, and heating is boiled and is stirred continuously, and prevents agar from gluing the bottom of a pan and liquid
It overflows, boils until agar is melted into liquid completely, then add glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, fill
Divide and stir evenly, dispense test tube while hot.During packing, try not that liquid medium is made to be sticked to test tube mouth, be such as stained with, use wet cloth
It cleans, in order to avoid adhere on tampon.Six test tubes are put into pressure cooker sterilizing, pressure 0.15MPa a bundle, and temperature is 121 DEG C of heat preservations 40
Minute, after the completion of sterilizing, Radix Astragali mother culture media is obtained, treats that kettle temperature is down to 60 DEG C, mother culture media is removed into pressure cooker,
Inclined-plane is placed, is inoculated with after anhydrous steam in test tube, test tube is put into superclean bench before inoculation, ultra violet lamp 30 divides
Clock；Ultraviolet lamp is closed, starts to be inoculated with, every test tube is inoculated with the Pleurotus nebrodensis parent species of 1 piece of a diameter of 0.5cm size, the examination after inoculation
Pipe is positioned in incubator and cultivates 7 ~ 14 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%.
（2）, make Radix Astragali functionality Pleurotus nebrodensis liquid spawn culture medium
200 grams of Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and cuts
200 grams of the potato of flakiness, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, removes potato block,
Filtrate is taken to use, 10 grams of brown sugar, 15 grams of sucrose, 3 grams of dusty yeast, vitamin B will be added in filtrate11 gram, 2.0 grams of magnesium sulfate,
2.0 grams of potassium dihydrogen phosphate, water 1000mL are sufficiently stirred, and are adjusted pH to 7.0, are dispensed into triangular flask, are put into pressure cooker sterilizing, pressure
Power 0.15MPa, temperature keep the temperature 40 minutes for 121 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium, treat in triangular flask
It being inoculated with after anhydrous steam, triangular flask is put into superclean bench before inoculation, ultra violet lamp 30 minutes closes ultraviolet lamp,
Start to be inoculated with, each 500mL triangular flasks connect 6 pieces of Radix Astragali Pleurotus nebrodensis parent species, the triangular flask after inoculation be put into shaking table with 150 turns/
Minute culture 10 days, 22 DEG C of cultivation temperature, humidity 50%.
（3）, make Radix Astragali functionality Cultivation of Pleurotus nebrodensis kind culture medium
300 grams of Radix Astragali leftover bits and pieces, 800 grams of corncob, thick 200 grams of sawdust, thin 200 grams of sawdust, 300 grams of wheat bran, 30 grams of lime are beautiful
50 grams of rice flour, pH value 7.5.
Radix Astragali leftover bits and pieces and sawdust are pressed 1 with water:1.5~1:1.7 ratios have been mixed, then the corncob crushed is immersed in
2 ~ 4 hours in 2% limewash add wheat bran, lime, corn flour and mix thoroughly after pulling out, composting 3 hours.It is adjusted before pack
PH, water content 65% have with hand-tight hold between webs under water droplet.
The culture medium high pressure sterilization for installing bag is kept for 4 hours at 125 DEG C, alternatively, normal-pressure sterilization will reach 100 DEG C in temperature
It is kept for 48 hours afterwards.
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, each cultivating bag（0.5kg siccatives）It is inoculated with 10mL liquid bacterias
Kind（Be shown experimentally that, Radix Astragali for Pleurotus nebrodensis mycelia growth inhibition unobvious, therefore inoculum concentration be 10mL, astragalus dispensing
Liquid spawn bacterium ball concentration 80%, 15/milliliter of bacterium ball number）.The cultivating bag for connecting bacterium is first put into culture in 28 DEG C of culturing room
It 24 hours, moves into 18 DEG C of culturing room and is cultivated until mycelia covers with bacterium bag after strain field planting.
（4）Fruiting
The bacterium bag for covering with mycelia is further cultured for 20 days, it is made further to complete physiological maturity, is then moved into mushroom room, is gone out
Mushroom manages, and control day temperature is 16 DEG C, and night temperatures are 5 DEG C, more than 85% humidity, and daytime controls illumination strong with sunshade net
Degree, thermal stimulation 15 days scratch bacterium bag after the long fruiting flower bud of bacterium bag, continue culture 14 days, you can the functional white spirit of picking Radix Astragali
Mushroom, entire mushroom producing culture period are 49 days.
（5）It is drying
By the fresh mushroom of harvesting, remove mushroom handle base portion impurity, be cut into the thin slice that thickness is 0.2cm, slice will longitudinal hacking, stem and bacterium
Lid is connected.The fresh mushroom of same day harvesting, same day chip drying obtain Radix Astragali functionality Pleurotus nebrodensis dry product.
After measured, the present embodiment cultivates to obtain single mushroom of Radix Astragali functionality Pleurotus nebrodensis and weighs 125 ~ 180 grams, average fresh mushroom production
For 0.65kg/kg siccatives, maintain an equal level compared with Radix Astragali yield is not added in culture medium.Through UV-VIS spectrophotometry and high-efficient liquid phase color
Spectrometry measures, pair that bioactive ingredients content is cultivated with ordinary culture medium in the Radix Astragali functionality Pleurotus nebrodensis sporophore cultivated
According to Pleurotus nebrodensis compared to there is different degrees of raising, detailed results are shown in Table 3.
Bioactive ingredients content in 3 Radix Astragali functionality Pleurotus nebrodensis sporophore of table
ND is not detected；N/A is not applied to.
Embodiment 4
A kind of Radix Astragali functional edible mushroom（Agaric）Production method, step is as follows：
（1）, make Radix Astragali functionality agaric parent species
Fetch earth 200 grams of beans respectively, 20 grams of agar, 20 grams of glucose, 2 grams of magnesium sulfate, 3 grams of potassium dihydrogen phosphate, 3 grams of peptone, yellow
50 grams of stilbene leftover bits and pieces, water 1000mL, pH7.0.
Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 2 hours, Radix Astragali leftover bits and pieces is pulled out, is then placed in and thinly slices
Potato, add water to 1000mL, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, removes potato
Piece takes filtrate to use, and filtrate is packed into aluminum pot, adds agar, and heating is boiled and is stirred continuously, and prevents agar from gluing the bottom of a pan and liquid
It overflows, boils until agar is melted into liquid completely, then add glucose, peptone, magnesium sulfate, potassium dihydrogen phosphate, fill
Divide and stir evenly, dispense test tube while hot.During packing, try not that liquid medium is made to be sticked to test tube mouth, be such as stained with, use wet cloth
It cleans, in order to avoid adhere on tampon.Six test tubes are put into pressure cooker sterilizing, pressure 0.15MPa a bundle, and temperature is 121 DEG C of heat preservations 40
Minute, after the completion of sterilizing, Radix Astragali mother culture media is obtained, treats that kettle temperature is down to 60 DEG C, mother culture media is removed into pressure cooker,
Inclined-plane is placed, is inoculated with after anhydrous steam in test tube, test tube is put into superclean bench before inoculation, ultra violet lamp 30 divides
Clock；Ultraviolet lamp is closed, starts to be inoculated with, every test tube is inoculated with the black fungus parent species of 1 piece of a diameter of 0.5cm size, the examination after inoculation
Pipe is positioned in incubator and cultivates 7 ~ 14 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%.
（2）, make Radix Astragali functionality black fungus liquid spawn culture medium
50 grams of Radix Astragali leftover bits and pieces in 90 DEG C or more water is impregnated 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and is cut into
200 grams of the potato of thin slice, heating is boiled, and is boiled until potato block is thoroughly well cooked but not mushy, with filtered through gauze, is removed potato block, is taken
Filtrate use, will be added in filtrate 10 grams of sucrose, 15 grams of glucose, 3 grams of peptone, vitamin B12 gram, 2.0 grams of magnesium sulfate,
2 grams of potassium dihydrogen phosphate, water 1000mL are sufficiently stirred, and are adjusted pH to 7.0, are dispensed into 250mL triangular flasks, are put into pressure cooker sterilizing,
Pressure 0.15MPa, temperature keep the temperature 40 minutes for 121 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium, treat triangular flask
It is inoculated with after middle anhydrous steam, triangular flask is put into superclean bench before inoculation, ultra violet lamp 40 minutes is closed ultraviolet
Lamp starts to be inoculated with, and each triangular flask connects 3 pieces of a diameter of 0.5cm sizes Radix Astragali black fungus parent species, and the triangular flask after inoculation, which is put into, to be shaken
It is cultivated 15 days with 150 revs/min in bed, 25 DEG C of cultivation temperature, humidity 60%.
The triangular flask liquid spawn made is accessed and further expands culture in fermentation tank（Culture substrate is matched in fermentation tank
Side is identical with Radix Astragali functionality black fungus liquid spawn culture medium formula）, be available for inoculation cultivating bag astragalus dispensing liquid
Body seed.
（3）, make Radix Astragali functionality cultivation of auricularia auricula kind culture medium
100 grams of Radix Astragali leftover bits and pieces, 1000 grams of cotton seed hull, thick 350 grams of sawdust, thin 300 grams of sawdust, 150 grams of wheat bran, lime are taken respectively
30 grams, 50 grams of corn flour, pH value 7.5.
Radix Astragali leftover bits and pieces and sawdust are pressed 1 with water:1.7 ratios have been mixed, and after being sufficiently humidified so as to it, then cotton seed hull and water are pressed
1:1.6 ratio has been mixed, and then adds in wheat bran, lime, corn flour by formula rate again, small with plastic film covering 4 after fully mixing thoroughly
When, material is allowed to suction moisture.Water content is adjusted before pack again 65% or so, i.e., with it is hand-tight hold between webs to have be advisable under water droplet.
The culture medium high pressure sterilization for installing bag is kept for 3 hours at 125 DEG C, alternatively, normal-pressure sterilization will reach 100 DEG C in temperature
It is kept for 48 hours afterwards.
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, each cultivating bag（0.5kg siccatives）It is inoculated with 15mL liquid bacterias
Kind（It is shown experimentally that, Radix Astragali is notable for the growth inhibition of black fungus mycelia, and inoculum concentration is small to cause black fungus mycelia to grow
It crosses slowly, inoculum concentration too conference causes bacterium bag humidity to increase, and miscellaneous bacteria grows, our experiments show that access amount 15mL is best access amount,
The black fungus astragalus dispensing liquid spawn bacterium ball concentration 80% of access, 20/milliliter of bacterium ball number）.The cultivating bag for connecting bacterium is first put
Enter in 28 DEG C of culturing room and cultivate 48 hours, move into 22 DEG C of culturing room and cultivated until mycelia covers with bacterium after strain field planting
Bag.
（4）Fruiting
The bacterium bag for covering with mycelia is further cultured for 20 days, it is made further to complete physiological maturity, is then moved into fruiting field, concentrates and draws
Mouthful, then bacteria 7 days, water spray urge ear to divide bed after 15 days again, carry out ear management, and day temperature is less than 25 DEG C, night temperatures
It is 15 DEG C, more than 85% humidity, daytime controls intensity of illumination with sunshade net, and Radix Astragali functionality black fungus can be picked after 15 days,
The entire mushroom producing culture period is 57 days.
（5）It is drying
By the fresh ear of harvesting, remove ear handle base portion impurity, auricle is put upward to be dried in sieve, not stirred, and waits to shine to auricle one
It carries out turning over ear drying, the agaric bunch that will can't not dry during drying again after the drying of face.Drying obtains Huang to after doing
Stilbene functionality black fungus dry product.
After measured, the present embodiment cultivates to obtain Radix Astragali functionality black fungus and is averaged fresh ear yield, the fresh ears of 1.0kg/kg siccatives,
Relatively being not added with the ordinary culture medium yield of Radix Astragali increases by 3.5%.It is surveyed through UV-VIS spectrophotometry and high performance liquid chromatography
It is fixed, in addition to Calycosin-7-O-BETA-D-glucoside and ononin, bioactive ingredients content in the Radix Astragali functionality agaric fructification cultivated
There is different degrees of raising compared with the control agaric that ordinary culture medium is cultivated, detailed results are shown in Table 4.
Bioactive ingredients content in 4 Radix Astragali functionality black fungus fructification of table
ND is not detected；N/A is not applied to.
It should be pointed out that for the those skilled in the art of the art, without departing from the principle of the present invention,
Several improvement and application can also be made, these are improved and application is also considered as protection scope of the present invention.
Claims (1)
1. a kind of production method of Radix Astragali functional edible mushroom, it is characterised in that：Include the following steps：
（1）, astragalus dispensing parent species make
Fetch earth 20 ~ 25 parts of 150 ~ 200 parts of beans, 25 ~ 30 parts of agar, glucose or sucrose, 1.5 ~ 2 parts of magnesium sulfate, di(2-ethylhexyl)phosphate respectively
2 ~ 3 parts of 1.5 ~ 2 parts of hydrogen potassium, peptone or dusty yeast, 25 ~ 75 parts of Radix Astragali crude drug or 50 ~ 150 parts of Radix Astragali leftover bits and pieces, water 1000
Part；
Radix Astragali leftover bits and pieces in 90 ~ 100 DEG C or more water is impregnated 2 ~ 4 hours, Radix Astragali leftover bits and pieces is pulled out, be then placed in be cut into it is thin
The potato of piece adds water and heats and boil, filter, agar is added in filtrate, and heating is boiled and is stirred continuously, boils complete to agar
Until being melted into liquid, glucose or sucrose, peptone or dusty yeast, magnesium sulfate, potassium dihydrogen phosphate are then added, is fully stirred
It mixes uniformly, dispenses test tube while hot；Pressure cooker sterilizing, 0.15 ~ 0.18MPa of pressure are put into, temperature is 120 ~ 125 DEG C of heat preservations 30 ~ 60
Minute, after the completion of sterilizing, obtain Radix Astragali mother culture media；It treats that temperature is down to 70 DEG C, mother culture media is removed into pressure cooker, place
Inclined-plane is inoculated with after anhydrous steam in test tube, and test tube is put into superclean bench before inoculation, ultra violet lamp 30 minutes,
Ultraviolet lamp is closed, starts to be inoculated with edible mushroom parent species, the test tube after inoculation is positioned in incubator and cultivates 7 ~ 14 days, cultivation temperature 20
~ 25 DEG C, humidity 40 ~ 80%；
（2）, astragalus dispensing liquid spawn making
Fetch earth 10 ~ 15 parts of 150 ~ 200 parts of beans, brown sugar or sucrose, 10 ~ 12 parts of glucose, 1.0 ~ 1.5 parts of magnesium sulfate, phosphoric acid respectively
1.5 ~ 2 parts of potassium dihydrogen, peptone or 2 ~ 3 parts of dusty yeast, vitamin B11 ~ 2 part, 25 ~ 100 parts of Radix Astragali crude drug or Radix Astragali are got a foothold
Expect 50 ~ 200 parts, 1000 parts of water, pH7.0；
Radix Astragali leftover bits and pieces in 90 ~ 100 DEG C or more water is impregnated 2 ~ 4 hours, Radix Astragali leftover bits and pieces is pulled out and is removed, is then placed in and cuts
The potato of flakiness, heating are boiled, filtering, and brown sugar or sucrose, glucose, peptone or dusty yeast, dimension life are added in filtrate
Plain B1, magnesium sulfate, potassium dihydrogen phosphate, be sufficiently stirred, be dispensed into triangular flask, be put into pressure cooker sterilizing, pressure 0.15 ~
0.18MPa, temperature keep the temperature 30 ~ 60 minutes for 120 ~ 125 DEG C, after the completion of sterilizing, obtain Radix Astragali liquid spawn culture medium；Treat triangle
It is inoculated with after anhydrous steam in bottle, triangular flask is put into superclean bench before inoculation, ultra violet lamp 30 ~ 60 minutes is closed
Ultraviolet lamp starts inoculation step（1）The Radix Astragali edible mushroom parent species of making, the triangular flask after inoculation are put into shaking table with 80 ~ 160
Rev/min culture 7 ~ 10 days, 20 ~ 25 DEG C of cultivation temperature, humidity 40 ~ 80%；
The triangular flask liquid spawn made is accessed and further expands culture in fermentation tank, obtains the Huang for being inoculated with cultivating bag
Stilbene formulation liquid seed；
（3）, astragalus dispensing cultigen making
50 ~ 150 parts of Radix Astragali crude drug or 100 ~ 300 parts of Radix Astragali leftover bits and pieces, corncob or 600 ~ 1000 parts of cotton seed hull, thick is taken respectively
150 ~ 300 parts of sawdust, 150 ~ 200 parts of thin sawdust, 150 ~ 300 parts of wheat bran, 10 ~ 30 parts of lime, 30 ~ 50 parts of corn flour, water content
60 ~ 65%, pH value 7.0 ~ 7.5；
When making the astragalus dispensing cultigen based on cotton seed hull, first Radix Astragali crude drug or Radix Astragali leftover bits and pieces and sawdust are pressed with water
1:1.5~1:1.7 ratios have been mixed, after being sufficiently humidified so as to it, then by cotton seed hull and water by 1:1.6~1:1.7 ratio has been mixed, then
Wheat bran, lime, corn flour are added in by formula rate again, is covered 2 ~ 5 hours with plastic film after fully mixing thoroughly；It is adjusted again before pack aqueous
Amount is 60 ~ 65%；
When making the astragalus dispensing cultigen based on corncob, first Radix Astragali crude drug or Radix Astragali leftover bits and pieces and sawdust are pressed with water
1:1.5~1:1.7 ratios have been mixed, then the corncob crushed is immersed in 2 ~ 4 hours in 1 ~ 2% limewash, after pulling out again
It adds in wheat bran, lime, corn flour to mix thoroughly, composting 1 ~ 3 hour；Adjustment pH, water content 60 ~ 68% before pack；
The culture medium high pressure sterilization for installing bag is kept for 2 ~ 4 hours at 121 ~ 126 DEG C, alternatively, normal-pressure sterilization will reach 100 in temperature
It is kept for 24 ~ 48 hours after DEG C；
Gone out bacterium cultivating bag cool down 48 hours after be inoculated with, in terms of 0.5kg siccatives each cultivating bag inoculation 10 ~ 15mL Radix Astragalis match
Square liquid spawn, wherein astragalus dispensing liquid spawn bacterium ball concentration 60 ~ 100%, 10 ~ 20/milliliter of bacterium ball number；Connect bacterium
Cultivating bag is put into 25 ~ 30 DEG C of culturing room and cultivates 24 ~ 48 hours, moves into 16 ~ 23 DEG C of culturing room and trains after strain field planting
It supports until mycelia covers with bacterium bag；
（4）, Radix Astragali functional edible mushroom cultivation management
The bacterium bag for covering with mycelia is further cultured for 15 ~ 30 days, it is made further to complete physiological maturity, is then moved into mushroom room, into
Row management of producing mushroom, control day temperature are 16 ~ 23 DEG C, and night temperatures are 5 ~ 15 DEG C, humidity 80 ~ 95%, sunshade network control on daytime
Intensity of illumination processed, 10 ~ 30 days long fruiting flower bud, continues culture 7 ~ 15 days, you can picking Radix Astragali functional edible mushroom, entire fruiting training
It is 32 ~ 75 days to support the period.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109429901A (en) * | 2018-12-14 | 2019-03-08 | 马山县山山农业科技有限公司 | The preparation method of edible fungus production increasing culture medium |
| CN111052982A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Liquid culture medium for Astragalus membranaceus mushroom and preparation method thereof |
| CN111052978A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Astragalus membranaceus mushroom cultivation method |
| CN112753486A (en) * | 2019-11-05 | 2021-05-07 | 中国医学科学院药用植物研究所 | Edible fungus culture medium containing astragalus stem leaves and using method |
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| CN104770222A (en) * | 2015-05-06 | 2015-07-15 | 青岛华盛绿能农业科技有限公司 | Method for inoculating stock spawn of edible fungi |
| CN106588279A (en) * | 2016-11-24 | 2017-04-26 | 颍上县唐垛湖现代农业科技有限公司 | Cultivation method of selenium-rich edible mushrooms |
| CN107667775A (en) * | 2017-08-08 | 2018-02-09 | 雷燕梅 | A kind of implantation methods of agrocybe |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104770222A (en) * | 2015-05-06 | 2015-07-15 | 青岛华盛绿能农业科技有限公司 | Method for inoculating stock spawn of edible fungi |
| CN106588279A (en) * | 2016-11-24 | 2017-04-26 | 颍上县唐垛湖现代农业科技有限公司 | Cultivation method of selenium-rich edible mushrooms |
| CN107667775A (en) * | 2017-08-08 | 2018-02-09 | 雷燕梅 | A kind of implantation methods of agrocybe |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111052982A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Liquid culture medium for Astragalus membranaceus mushroom and preparation method thereof |
| CN111052978A (en) * | 2018-10-16 | 2020-04-24 | 郭立潮 | Astragalus membranaceus mushroom cultivation method |
| CN109429901A (en) * | 2018-12-14 | 2019-03-08 | 马山县山山农业科技有限公司 | The preparation method of edible fungus production increasing culture medium |
| CN112753486A (en) * | 2019-11-05 | 2021-05-07 | 中国医学科学院药用植物研究所 | Edible fungus culture medium containing astragalus stem leaves and using method |
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Application publication date: 20180622 |