CN101228829B - Culture method of black fungus - Google Patents

Culture method of black fungus Download PDF

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CN101228829B
CN101228829B CN2007101593536A CN200710159353A CN101228829B CN 101228829 B CN101228829 B CN 101228829B CN 2007101593536 A CN2007101593536 A CN 2007101593536A CN 200710159353 A CN200710159353 A CN 200710159353A CN 101228829 B CN101228829 B CN 101228829B
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parts
bacterium
water
days
gypsum
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CN2007101593536A
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CN101228829A (en
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顾英梅
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顾英梅
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/10Process efficiency
    • Y02P20/133Renewable energy sources, e.g. sunlight

Abstract

A cultivating method for an Auricularia auricular belongs to the cultivating technology of edible fungi, which comprises the steps of inoculation and cultivation of the first grade fungus, inoculation and cultivation of the second grade fungus, inoculation and cultivation of the third grade fungus, accelerating germination and growing to ears. The culture medium of the strain is made of broadleafsawdust, soybean powder, gypsum, straw powder, wheat bran, white sugar, gypsum, lime and food grains, etc. The Auricularia auricular cultivated with the method is of large ear, thick flesh, rich nutrition and good taste, which has good shapes after the sun drying, thus having a good market prospect. In addition, with easy access to raw materials, low cost, short startup time and no land occupation, the method can be used in urban and rural areas with various conditions, which is not only suitable for factory management, but also suitable for household production.

Description

A kind of cultivating method of woodear
Technical field
The invention belongs to cultivation technology of edible fungi, particularly relate to a kind of cultivating method of woodear.
Background technology
Woodear one small part in the market is that collection is wild, and the overwhelming majority is tame.Artificial cultivation has dual mode: a kind of is to fell 10~20 years living broad-leaved trees to carry out artificial juggle cultivation, another kind is the cultivation that is packed in bag, this dual mode all has its deficiency, the juggle fungus cultivation heavy damage forest resources, though and existing bag cultivating has been saved the forest resources, but because of woodear temperature, humidity are waited until that external condition is strict, its auricularia auriculajudae is yielded poorly.
Summary of the invention
The present invention is intended to overcome the deficiencies in the prior art, has developed a kind of cultivating method of woodear.
The cultivating method of a kind of woodear of the present invention, specifically finish by following operating procedure:
The inoculation of a, one-level bacterium with cultivate: will prepare the one-level bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 40 minutes, reduces to normal temperature again; The one-level bacterium inoculates, and female kind of the auricularia auriculajudae of purifying inserted in the medium, and its control temperature is between 24~26 ℃, grew 15~20 days, promptly finishes the cultivation of one-level bacterium;
B, inoculation of secondary bacterium and cultivation: will prepare the secondary level bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is to normal temperature, with a step is that the one-level bacterium that makes is inserted in the secondary bacterium culture medium, in culturing room, grew 30~50 days, humidity is controlled at 45~60%, the indoor lucifuge that needs, temperature remained on 24~27 ℃ in wherein preceding 15~25 days, 15~25 days temperature in back remain on 20~23 ℃, again through 10~15 ℃ of room temperatures, 7~10 days dormancy, the secondary bacterium is cultivated and finishes;
C, three grades of bacterium inoculate and cultivation: will prepare three grades of bacterium culture mediums finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is that the secondary bacterium that makes is inserted in three grades of bacterium culture mediums to normal temperature with the b step; Bacteria, be to be to carry out under 45~60% conditions in lucifuge, relative moisture, its temperature be preceding 7~10 days be to be 23~26 ℃ after 26~28 ℃, 10 days, treat that mycelia reached for half growth stage, room temperature is reduced to 20~23 ℃, and three grades of bacterium were cultivated and finish when cultivation whole process was 30~40 days;
G, vernalization and growth: the mycelia that grows up to, be 75~85%, carry out appropriate illumination in 10~20 ℃ of temperature maintenances, humidity, promptly on the medium of c step, grew the ear bud through 7~15 days, grow and can gather in the crops into ear in 45~65 days;
The preparation method of aforesaid one-level bacterium culture medium is:
Comprise by its parts by weight batching: 180~220 parts of potatos, 17~22 parts of powdered glucoses, 17~22 parts in agar, 1400~1600 parts in water and vitamin B1 are 0.003~0.02 part, its compound method is potato to be cut into fragment add water boil, 10~20 minutes leaching juice, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product, or
Comprise by its parts by weight batching: 180~220 parts of 800~1200 parts in wheat bran, 800~1200 parts in water, 180~220 parts in agar and powdered glucoses, its compound method is to add the wheat bran infusion after heating water to 100 ℃, leaching juice after half an hour, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product;
The preparation method of aforesaid secondary bacterium culture medium is:
Comprise by its parts by weight batching: 72~80 parts of broad-leaved wood chips, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime, 0.3~0.7 part of white sugar, 17~22 parts in wheat bran and water, or
Comprise by its parts by weight batching: 74~82 parts of broad-leaved wood chips, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 72~78 parts in straw powder, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum, 0.5~2 part in lime, 1~3 part of soybean cake powder and water, or
Comprise by its parts by weight batching: 85~94 parts of grain, wheat bran or 8~13 parts of chaffs, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 64~72 parts of grain, 17~22 parts of broad-leaved wood chips, wheat bran or 8~13 parts of chaffs, 1~3 part in gypsum, 0.3~0.7 part of salt and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from;
The preparation method of aforesaid three grades of bacterium culture mediums is:
Comprise by its parts by weight batching: 82~90 parts of broad-leaved wood chips, wheat bran or 8~13 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water, or
Comprise by its parts by weight batching: 25~35 parts of maize cob meals, 40~60 parts in straw powder, 1.5~3 parts of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts in straw powder, wheat bran or 22~29 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts of corn straw powders, 22~28 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from.
The cultivating method of woodear of the present invention is fit to the female cultivation of planting of any woodear, can be buied or other commercially femalely plants, also can publish in December, 1999 according to Chinese agriculture publishing house female kind of method self-control of 144~146 pages of records in " the eating bacterium production technology pandect " of distribution, Chen Shiyu by Heilongjiang Province's using microbe research institute for its female kind.
Of the present inventionly connect bacterium several different methods is arranged, as connecing the bacterium chamber, indoorly want thorough disinfection, connect bacterium and ultraviolet ray, alcolhol burner, tweezers, alcohol, bacterium hook etc. are arranged with ion wind (also cry and connect the bacterium device) is indoor indoor.Used apparatus is put into indoor, carries out indoor sterilization the bag bacterium cooling, and common disinfectants has; Mushroom protects, and aerosols such as mushroom power god need to use by explanation.Aerosol is lighted and smog will be occurred, and light back chamber door and will seal, laggard people's work half an hour, the staff receives the secondary bacterium and seals suitable for readingly in the bacterium bag, carries out bacteria.Connect the also available case of bacterium, in the good packed cartonning of cooling, utensil (tweezers, alcohol, bacterium hook, lighter, the rubber gloves bacterial classification) carries out disinfection, disinfectant will use by explanation, lights and just can operate in back 30 minutes, and the staff will pitch both hands in the cartonning, be with gloves earlier, clean gloves with alcohol swab, bacterial classification is being put into bag, the cotton jam-pack with sterilization suitable for reading.Connect the also available tweezers of bacterium folder bacterial classification and implant in the bag, or with the bacterium hook also can, usefulness cotton jam-pack suitable for reading is just passable, connect one the case after, open case the bacterium bag transported on the bacteria frame of bacteria chamber.
The water in mountain area is different with the water in Plain, and the mountain area rainwater is big, the natural air high humidity, and auricularia auriculajudae is watered just long very fast of a water, and mountain area wind is little, watered water and can not do very soon, and near spring, river waters.Water is upsurge, waters the back auricularia auriculajudae and is good at very much.But the Plain difference, wind is big, and is dry, do not have scenery with hills and waters.There is not river.As long as underground deep water waters, but the underground water cold, as long as 5 degree do not possess the growth conditions of auricularia auriculajudae, so water is carried out hyperthermic treatment.Underground water mentioned in drought resisting bucket or the hole be exposed to the sun, water can nature heats up.The delivery port of pouring will filter the red scale in the filtered water with boiler ash sediment and crushed stone.Just can pluck airing, airing screen window, bed, sunshade net in 60-70 days with adding the warm water pouring.Shine auricle earlier, tedding the basal part of the ear.Just can dry in 2-3 days.
The flat area cultivating black fungus has a lot of advantages than the mountain area, because the vegetative period of forest zone auricularia auriculajudae is short, physioclimate high humidity rainwater is too diligent, the auricularia auriculajudae fast growth of urging, and the auricularia auriculajudae auricle is big, ear thin, is prone to the stream ear, mashed ear phenomenon.If the mountain area runs into arid, there is not the water source pouring, the output of auricularia auriculajudae will be affected.And the Plain is just different, and Plain wind heavy rain is few, and is dry, use missile silo water, the manual watering increases humidity, and growth is slow, do not avoid the stream ear, mashed ear phenomenon, growth time is longer than the mountain area, the auricularia auriculajudae sheet that grows is big, meat is thick, nutritious, mouthfeel good, auricularia auriculajudae is shone the shapeliness that, and good market prospects are arranged.And raw material is easy to get, cost is low, produce effects soon, do not occupy cultivated land, and town and country can utilize that various conditions are cultivated, its both had been fit to factory management, also can single household have produced.
Embodiment
Embodiment 1
A kind of cultivating method of woodear, finish by following concrete operations step:
The inoculation of a, one-level bacterium with cultivate: will prepare the one-level bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 40 minutes, reduces to normal temperature again; The one-level bacterium inoculates, and female kind of the auricularia auriculajudae of purifying inserted in the medium, and its control temperature is between 24~26 ℃, grew 15~20 days, promptly finishes the cultivation of one-level bacterium;
B, inoculation of secondary bacterium and cultivation: will prepare the secondary level bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is to normal temperature, with a step is that the one-level bacterium that makes is inserted in the secondary bacterium culture medium, in culturing room, grew 30~50 days, humidity is controlled at 45~60%, the indoor lucifuge that needs, temperature remained on 24~27 ℃ in wherein preceding 15~25 days, 15~25 days temperature in back remain on 20~23 ℃, again through 10~15 ℃ of room temperatures, 7~10 days dormancy, the secondary bacterium is cultivated and finishes;
C, three grades of bacterium inoculate and cultivation: will prepare three grades of bacterium culture mediums finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is that the secondary bacterium that makes is inserted in three grades of bacterium culture mediums to normal temperature with the b step; Bacteria, be to be to carry out under 45~60% conditions in lucifuge, relative moisture, its temperature be preceding 7~10 days be to be 23~26 ℃ after 26~28 ℃, 10 days, treat that mycelia reached for half growth stage, room temperature is reduced to 20~23 ℃, and three grades of bacterium were cultivated and finish when cultivation whole process was 30~40 days;
G, vernalization and growth: the mycelia that grows up to, be 75~85%, carry out appropriate illumination in 10~20 ℃ of temperature maintenances, humidity, promptly on the medium of c step, grew the ear bud through 7~15 days, grow and can gather in the crops into ear in 45~65 days;
The preparation method of aforesaid one-level bacterium culture medium is:
Comprise by its parts by weight batching: 180~220 parts of potatos, 17~22 parts of powdered glucoses, 17~22 parts in agar, 1400~1600 parts in water and vitamin B1 are 0.003~0.02 part, its compound method is potato to be cut into fragment add water boil, 10~20 minutes leaching juice, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product, or
Comprise by its parts by weight batching: 180~220 parts of 800~1200 parts in wheat bran, 800~1200 parts in water, 180~220 parts in agar and powdered glucoses, its compound method is to add the wheat bran infusion after heating water to 100 ℃, leaching juice after half an hour, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product;
The preparation method of aforesaid secondary bacterium culture medium is:
Comprise by its parts by weight batching: 72~80 parts of broad-leaved wood chips, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime, 0.3~0.7 part of white sugar, 17~22 parts in wheat bran and water, or
Comprise by its parts by weight batching: 74~82 parts of broad-leaved wood chips, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 72~78 parts in straw powder, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum, 0.5~2 part in lime, 1~3 part of soybean cake powder and water, or
Comprise by its parts by weight batching: 85~94 parts of grain, wheat bran or 8~13 parts of chaffs, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 64~72 parts of grain, 17~22 parts of broad-leaved wood chips, wheat bran or 8~13 parts of chaffs, 1~3 part in gypsum, 0.3~0.7 part of salt and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from;
Aforesaid three grades of bacterium culture medium preparation methods are:
Comprise by its parts by weight batching: 82~90 parts of broad-leaved wood chips, wheat bran or 8~13 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water, or
Comprise by its parts by weight batching: 25~35 parts of maize cob meals, 40~60 parts in straw powder, 1.5~3 parts of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts in straw powder, wheat bran or 22~29 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts of corn straw powders, 22~28 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from
Embodiment 2
A kind of cultivating method of woodear, finish by following concrete operations step:
The inoculation of a, one-level bacterium with cultivate: will prepare the one-level bacterium culture medium of finishing and place in vitro, put into pressure cooker and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 40 minutes, reduces to normal temperature again; The one-level bacterium inoculates, and female kind of the auricularia auriculajudae of purifying inserted in the medium of test tube, and its control temperature is between 24~26 ℃, grew 15~20 days, promptly finishes the cultivation of one-level bacterium;
B, secondary bacterium inoculation with cultivate: will prepare the secondary level bacterium culture medium of finishing and pack in bacterium bag or the bottle, place the normal-pressure sterilization pot to carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is to normal temperature, with a step is that the one-level bacterium that makes is inserted in the secondary bacterium culture medium, place culturing room to grow 30~50 days in bacterium bag or bottle, humidity is controlled at 45~60%, the indoor lucifuge that needs, temperature remained on 24~27 ℃ in wherein preceding 15~25 days, 15~25 days temperature in back remain on 20~23 ℃, again through 10~15 ℃ of room temperatures, 7~10 days dormancy, the secondary bacterium is cultivated and finishes; But the resting period can not be long, and the time has been grown bacterial classification and can wear out, and the mycelia surface has the yellow globule, has viscosity, and mycelia will dried-uply shrink, a little less than the resistance against diseases,
C, the inoculation of three grades of bacterium and cultivate: will prepare three grades of bacterium culture mediums finishing pack in the bacterium bag, with the bacterium rod jam-pack suitable for reading, also available no cotton collarium and lid envelope suitable for reading tightly after, place the normal-pressure sterilization pot to carry out sterilization treatment, sterilising temp is 100~120 ℃, continues 8~10 hours, after stopping to heat 4 hours, the bacterium bag is moved to the indoor cold of drying in the air to normal temperature from autoclave, be that the secondary bacterium that makes is inserted in three grades of bacterium culture mediums with the b step; Bacteria, will sterilize in the previous day in the bacteria chamber, make room temperature at 28~30 ℃, relative moisture 70%, use aerosol, strengthen one times dose by explanation, it is more thorough to sterilize, the bacterium bag is moved in the bacteria chamber, in lucifuge, relative moisture is to carry out bacteria under 45~60% conditions, its temperature be preceding 7~10 days be to be 23~26 ℃ after 26~28 ℃, 10 days, treat that it is that mycelia is long during to half bag that mycelia reached for half growth stage, room temperature is reduced to 20~23 ℃, and cultivating whole process is that three grades of bacterium of promptly finishing in 30~40 days are cultivated;
G, vernalization and growth: behind the mycelia that the bacterium bag grows up to, punch with the V-arrangement cutter and to dig mouth, keep 10~20 ℃, humidity to be 75~85%, to carry out appropriate illumination, promptly on the medium of bacterium bag, grew the ear bud through 7~15 days, grow and to gather in the crops into ear in 45~65 days in temperature;
The preparation method of aforesaid one-level bacterium culture medium is: comprise by its parts by weight batching: 180~220 parts of potatos, 17~22 parts of powdered glucoses, 17~22 parts in agar, 1400~1600 parts in water and vitamin B1 are 0.003~0.02 part, its compound method is potato to be cut into fragment add water boil, 10~20 minutes leaching juice, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product
The preparation method of aforesaid secondary bacterium culture medium is hybridly prepared into siccative for 17~22 parts with 0.3~0.7 part of 0.3~0.7 part in 0.5~2 part in 1~3 part of 72~80 parts of broad-leaved wood chips, soybean cake powder, gypsum, lime, the white sugar of parts by weight and wheat bran, and the water that adds siccative total amount 120~150% again stirs and makes;
Aforesaid three grades of bacterium culture medium preparation methods are hybridly prepared into siccative for 0.3~0.7 part with 82~90 parts of the broad-leaved wood chips of parts by weight, wheat bran or 8~13 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum and lime, and the water that adds siccative total amount 120~150% again stirs and makes.
Embodiment 3
A kind of cultivating method of woodear, its
The one-level bacterium culture medium comprises by its parts by weight batching: 200 parts of 1000 parts in wheat bran, 1000 parts in water, 200 parts in agar and powdered glucoses, its compound method is to add the wheat bran infusion after heating water to 100 ℃, leaching juice after half an hour, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product;
The secondary bacterium culture medium comprises by its parts by weight batching: 78 parts of broad-leaved wood chips, 20 parts in wheat bran, 1 part of white sugar, 1 part in gypsum and water, its preparation method are that above-mentioned each prescription all needs siccative is mixed, and add 120% water by siccative weight again and stir and be prepared from;
Three grades of bacterium culture mediums comprise by its parts by weight batching: 30 parts of maize cob meals, 50 parts of straw pulverizing, 2 parts of soybean cake powder, 1 part in gypsum, 0.7 part in lime and water, its preparation method is that above-mentioned each prescription all needs siccative is mixed, and adds 150% water by siccative weight again and stirs and be prepared from.
Other operation working condition is identical with embodiment 2.
Set forth component, prescription and the preparation method of different medium below respectively:
(1) the present invention prepares the prescription and the parts by weight of one-level bacterium culture medium and is:
Table one: table two:
Wherein: the preparation method of medium is identical with the preparation method of one-level bacterium culture medium among the embodiment 2 in the table one;
The preparation method of medium is identical with the preparation method of one-level bacterium culture medium among the embodiment 3 in the table two.
(2) the present invention prepares the siccative prescription and the parts by weight of secondary bacterium culture medium and is:
Table one: table two:
Table three: table four:
Table five:
The preparation method that table one is respectively organized the secondary bacterium culture medium to the table five is identical with the preparation method of secondary bacterium culture medium among the embodiment 2.
(3) the present invention prepares the siccative prescription and the parts by weight of three grades of bacterium culture mediums and is:
Table one: table two:
Table three: table four:
The preparation method that table one is respectively organized three grades of bacterium culture mediums to the table four is identical with the preparation method of three grades of bacterium culture mediums among the embodiment 2.

Claims (1)

1. the cultivating method of a woodear, finish by following concrete operations step:
The inoculation of a, one-level bacterium with cultivate: will prepare the one-level bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 40 minutes, reduces to normal temperature again; The one-level bacterium inoculates, and female kind of the auricularia auriculajudae of purifying inserted in the medium, and its control temperature is between 24~26 ℃, grew 15~20 days, promptly finishes the cultivation of one-level bacterium;
B, inoculation of secondary bacterium and cultivation: will prepare the secondary bacterium culture medium of finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air is to normal temperature, the one-level bacterium that a step makes is inserted in the secondary bacterium culture medium, grow in culturing room 30~50 days, humidity are controlled at 45~60%, the indoor lucifuge that needs, temperature remained on 24~27 ℃ in wherein preceding 15~25 days, 15~25 days temperature in back remain on 20~23 ℃, through 10~15 ℃ of room temperatures, 7~10 days dormancy, the secondary bacterium is cultivated and finishes again;
C, three grades of bacterium inoculate and cultivation: will prepare three grades of bacterium culture mediums finishing and carry out sterilization treatment, sterilising temp is 100~120 ℃, constant temperature 8~10 hours, stop to heat the nature cooling and continue 4 hours, the indoor cold of drying in the air inserts the secondary bacterium that the b step makes in three grades of bacterium culture mediums to normal temperature; Bacteria, be to be to carry out under 45~60% conditions in lucifuge, relative moisture, its temperature be preceding 7~10 days be to be 23~26 ℃ after 26~28 ℃, 10 days, treat that mycelia reached for half growth stage, room temperature is reduced to 20~23 ℃, and three grades of bacterium are cultivated and finish when cultivating whole process to 30~40 days;
D, vernalization and growth: the mycelia that grows up to is 75~85%, carries out appropriate illumination that in 10~20 ℃ of temperature maintenances, humidity promptly grew the ear bud on the medium of c step through 7~15 days, regrowth can be gathered in the crops into ear in 45~65 days;
The preparation method of aforesaid one-level bacterium culture medium is:
Comprise by its parts by weight batching: 180~220 parts of 800~1200 parts in wheat bran, 800~1200 parts in water, 180~220 parts in agar and powdered glucoses, its compound method is to add the wheat bran infusion after heating water to 100 ℃, leaching juice after half an hour, again juice and other batch mixes in the prescription are mixed all, continue heating again, enduring of agar got final product;
The preparation method of aforesaid secondary bacterium culture medium is:
Comprise by its parts by weight batching: 72~80 parts of broad-leaved wood chips, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime, 0.3~0.7 part of white sugar, 17~22 parts in wheat bran and water, or
Comprise by its parts by weight batching: 74~82 parts of broad-leaved wood chips, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 72~78 parts in straw powder, 17~22 parts in wheat bran, 0.5~2 part of white sugar, 0.5~2 part in gypsum, 0.5~2 part in lime, 1~3 part of soybean cake powder and water, or
Comprise by its parts by weight batching: 85~94 parts of grain, wheat bran or 8~13 parts of chaffs, 0.5~2 part in gypsum and water, or
Comprise by its parts by weight batching: 64~72 parts of grain, 17~22 parts of broad-leaved wood chips, wheat bran or 8~13 parts of chaffs, 1~3 part in gypsum, 0.3~0.7 part of salt and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from;
Aforesaid three grades of bacterium culture medium preparation methods are:
Comprise by its parts by weight batching: 82~90 parts of broad-leaved wood chips, wheat bran or 8~13 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water, or
Comprise by its parts by weight batching: 25~35 parts of maize cob meals, 40~60 parts in straw powder, 1.5~3 parts of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts in straw powder, wheat bran or 22~29 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.5~1 part in lime and water, or
Comprise by its parts by weight batching: 66~74 parts of corn straw powders, 22~28 parts in rice bran, 1~3 part of soybean cake powder, 0.5~2 part in gypsum, 0.3~0.7 part in lime and water,
Above-mentioned each prescription all needs siccative is mixed, and adds 120~150% water by siccative weight again and stirs and be prepared from.
CN2007101593536A 2007-12-30 2007-12-30 Culture method of black fungus CN101228829B (en)

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CN102690141B (en) * 2012-01-15 2013-12-25 河南科技大学 Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus
CN103408350B (en) * 2013-07-08 2014-12-10 西北农林科技大学 Culture medium for edible fungi
CN103688758B (en) * 2013-12-15 2016-03-16 张爱美 A kind of original ecology cultivating method of Chinese scholartree ear
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