CN104303825A - Method for cultivating selenium-enriched agrocybe cylindracea - Google Patents

Method for cultivating selenium-enriched agrocybe cylindracea Download PDF

Info

Publication number
CN104303825A
CN104303825A CN201410501834.0A CN201410501834A CN104303825A CN 104303825 A CN104303825 A CN 104303825A CN 201410501834 A CN201410501834 A CN 201410501834A CN 104303825 A CN104303825 A CN 104303825A
Authority
CN
China
Prior art keywords
selenium
agrocybe
mushroom
raw material
agrocybe cylindracea
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410501834.0A
Other languages
Chinese (zh)
Other versions
CN104303825B (en
Inventor
魏云辉
李宁
陈庆隆
胡中娥
王洪秀
熊涛
熊小文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangchang Zhenglian Biotechnology Co ltd
Original Assignee
Research Institute Of Agricultural Application Of Microorganisms Of Jiangxi Academy Of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Institute Of Agricultural Application Of Microorganisms Of Jiangxi Academy Of Agricultural Sciences filed Critical Research Institute Of Agricultural Application Of Microorganisms Of Jiangxi Academy Of Agricultural Sciences
Priority to CN201410501834.0A priority Critical patent/CN104303825B/en
Publication of CN104303825A publication Critical patent/CN104303825A/en
Application granted granted Critical
Publication of CN104303825B publication Critical patent/CN104303825B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/10Mycorrhiza; Mycorrhizal associations
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00Mixtures of fertilisers belonging individually to different subclasses of C05
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses a method for cultivating selenium-enriched agrocybe cylindracea. The method comprises the following steps of selecting agrocybe cylindracea bacterial strains which are selenium salt resistant and have high bio-concentration on selenium, and performing selenium salt resistant and selenium enriching domestication on the strains; preparing an agrocybe cylindracea selenium-enriching intensifying culture substrate material, bagging, sterilizing and inoculating the culture substrate material, and culturing agrocybe cylindracea mycelia; after the agrocybe cylindracea mycelia are physiologically mature, transferring agrocybe cylindracea fungi bags into a fruiting room for fruiting management, performing inducement to primordium and agrocybe cylindracea cultivation by adjusting agrocybe cylindracea growth temperature, humidity, ventilation and light conditions, promoting the growth, development and the bio-concentration on the selenium of the agrocybe cylindracea, and finally producing the selenium-enriched agrocybe cylindracea integrating nutritional, medical and health-care effects of the agrocybe cylindracea and good effects of organic selenium. The method is easy and convenient to operate and easy to implement, the selenium enriching effect is obvious, the cultivation material is sufficient, the cost is low, the cultivation area is large, the social and economic benefits are obvious, and the application prospect is wide.

Description

A kind of method of selenium enriched tea mushroom cultivation
Technical field
The invention belongs to bacterium industry field, be specifically related to the method for a kind of selenium enriched tea mushroom cultivation.
Background technology
Agrocybe delicious flavour, nutritious, there is higher edible and medical value.Selenium is one trace element necessary in humans and animals body, to human body, there is much important effect, the shortage of selenium can directly cause Keshan disease and Kaschin-Beck disease, the extensive Problems existing in the soil Que Xishi world, 72% of China's area belongs to scarce selenium and low selenium area, sufficient selenium cannot be obtained in the process of growth of the crops in these areas, thus form the generally scarce selenium state of food chain from soil.Selenium-enriched food of ingesting has become the important means ensureing national health at present.But the toxicity of inorganic selenium is far longer than Organic Selenium, edible mushroom is one of best rich selenium carrier, a large amount of research and practice proves, agrocybe has extremely strong enrichment to selenium, by carrying out selenium nutrition-intensified to it, the a large amount of enrichment in tea plant mushroom fruit body of selenium element can be made to close biological transformation, improve the content of tea plant mushroom fruit body Organic Selenium.
The object of this method is to provide a kind of cultivation method of selenium enriched tea mushroom, produce the selenium enriched tea mushroom integrating agrocybe idiotrophic, medical treatment, health care and Organic Selenium excellent efficacy, there is provided an important channel for human body supplements efficient, safe and reliable Organic Selenium, and produce significant economic benefit and social benefit.
Summary of the invention
Method object provided by the invention is a kind of method providing selenium enriched tea mushroom to cultivate, take agrocybe as carrier, select salt tolerant and the strong cultivated strains of selenium enriching functions, and the salt of resistance to selenium and the domestication of rich selenium are carried out to it, add external source selenium, prepare rich selenium intensified culturing matrix, comprehensive regulation temperature, humidity, ventilation, the agrocybe growth conditionss such as illumination, promote the biological concentration of selenium element in tea plant mushroom fruit body, improve the content of Organic Selenium in agrocybe, produce collection agrocybe idiotrophic, medical treatment, the selenium enriched tea mushroom that health care and Organic Selenium excellent efficacy are integrated, for human body supplements efficient, safety, reliable Organic Selenium provides an important channel.
For achieving the above object, the present invention takes following technical scheme:
A kind of method of selenium enriched tea mushroom cultivation, first select the salt of resistance to selenium ability and the domestication of the salt of resistance to selenium and selenium rich ability carried out it to the strong agrocybe aegerita KMFJ-FC of selenium biological concentration, then the rich selenium enriched medium of agrocybe is prepared, medium is packed, sterilizing, inoculation, cultivate agrocybe mycelium, after agrocybe mycelium physiological ripening, agrocybe bacterium bag is moved into mushroom room and carries out management of producing mushroom, pass through regulating and controlling temperature, humidity, the agrocybe growth conditionss such as ventilation and illumination, carry out agrocybe and urge flower bud, educate mushroom, promote agrocybe growing to the enrichment of selenium and fruit body, final production goes out to collect agrocybe idiotrophic, medical treatment, the selenium enriched tea mushroom that health care and Organic Selenium excellent efficacy are integrated.
Its concrete steps are as follows:
1. the salt of resistance to selenium ability and the selenium enriched tea mushroom cultivated strains strong to selenium biological concentration is selected.Jiangxi tea AS-1 is selected to be agrocybe selenium-enriching cultivation bacterial strain, and it is carried out to the domestication of the salt of resistance to selenium and selenium rich ability, during domestication, domestication liquid sodium selenite aqueous solution is added in original seed and cultivated species domestication medium and carry out inoculating and cultivating, original seed and Cultivar culture medium raw material and mass fraction thereof are: wood chip 35%, wheat bran 25%, cotton seed hulls 33%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, and add water to it and make its water content 60% ~ 65%, in the wild Oryza species that adds water, sodium selenite mass fraction is 0.04%;
2. rich selenium intensified culturing based raw material is prepared.When preparing rich selenium intensified culturing based raw material, adding sodium selenite powder is external source selenium, with natural selenium-rich raw material weed tree sawdust and wheat bran for primary raw material, adds 5% corn flour, improve the content of protein in cultivation base raw material, strengthen agrocybe mycelium to the tolerance of selenium source.Rich selenium intensified culturing based raw material and mass fraction thereof are: wood chip 35%, wheat bran 25%, cotton seed hulls 32%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, add water to it and make its water content 60% ~ 65%, after adding water, in cultivation base raw material, sodium selenite mass fraction is 0.045 ~ 0.065%;
3. pack, sterilizing.The mixing of the raw material of rich selenium intensified culturing base mixed thoroughly, adjustment moisture content in medium to 60% ~ 65%, adopts 12cm × 24cm size polyethylene normal pressure plastic sack or the packed bag of polypropylene high-pressure plastic.Carry out atmospheric steam sterilizing or high pressure steam sterilization.During atmospheric steam sterilizing, keep 12h ~ 14h when autoclave temperature reaches 98 DEG C ~ 100 DEG C, during high pressure steam sterilization, when 121 DEG C ~ 123 DEG C, keep 3h ~ 4h.
4. inoculate, send out bacterium.When pocket temperature after subject to sterilization drops to below 60 DEG C, be transported to by pocket in the transfer room after sterilization or inoculating hood, require to inoculate according to sterile working, every bag cultivating kind connects 30 bags ~ 40 Bag Material bags.Postvaccinal bacterium bag is transported in culturing room to be cultivated, and culturing room's temperature controls at about 22 DEG C, and once, each 30min ~ 40min, keeps indoor air relative humidity 60% ~ 70%, carry out shading cultivation in each ventilation sooner or later.
5. urge flower bud, educate mushroom.Bacterium bag is cultivated 50-60 days, mycelia covers with bacterium bag, the high resilience of bacterium bag, mycelia secretion pigment tells yellow water, during the mycelia band point brown of sack surface, take " four is poor " stimulus method (temperature difference, psychrometric difference, oxygen concentration are poor, illumination is poor) to carry out urging flower bud and educating mushroom, promote the biological concentration of selenium element in tea plant mushroom fruit body.
Concrete grammar is:
After mycelia is covered with bag and starts to tell the yellow globule, sack is stretching, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C ~ 22 DEG C, continuous 5d ~ 7d widens day and night temperature, the temperature difference is made to reach 6 DEG C ~ 8 DEG C, impel a large amount of former base to occur, temperature is brought up to 22 DEG C ~ 26 DEG C to the sporophore growth stage after buddingging, impel agrocybe stem long long thick.Psychrometric difference stimulates, before fruiting, every morning is to space spray water, relative air humidity brings up to 85% ~ 90% by 70%, build mushroom room dry wet alternating environment, in the sporophore growth stage, directly to sack water spray, relative air humidity remains on 90% ~ 95%, impel tea plant mushroom fruit body normal growth, before gathering, 1d stops water spray.Oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min ~ 40min, fruit body grow to five points ripe time, ventilate every day 1 time, ventilation time 20min.Illumination difference stimulates, and after mycelium physiological ripening, intensity of illumination is increased to 500Lex ~ 800 Lex, light stimulation 5d ~ 7d, until former base is formed and buddings.In the sporophore growth later stage, intensity of illumination is reduced to 200Lex ~ 500Lex, impels stem to bleach and cap dark brown, improve agrocybe commodity value.
6. gather.Pluck when agrocybe grows to 7-8 point of maturation, pluck 4 batches ~ 5 batches mushrooms according to above-mentioned management of producing mushroom method.
Remarkable advantage of the present invention is:
1. filter out the salt of resistance to selenium and be cultivated strains to selenium bioaccumulation ability strong Jiangxi tea AS-1, and the salt of resistance to selenium and the domestication of rich selenium are carried out to it, improve the cultivated strains salt of resistance to selenium ability and to selenium bioconcentration, concentration effect is remarkable;
2. adding commercially available sodium selenite powder is external source selenium, with natural selenium-rich raw material weedtree wood chip and wheat bran for selenium source carrier, improve weedtree wood chip and the wheat bran content at culture medium for cultivating, improve the content of protein in culture medium for cultivating, improve agrocybe mycelia to selenium source tolerance, prepare rich selenium intensified culturing matrix, raw material are extensive, with low cost;
3. urging flower bud and educating the mushroom phase, " four is poor " stimulus method (temperature difference, psychrometric difference, oxygen concentration are poor, illumination is poor) is taked to carry out urging flower bud and educating mushroom, promote that tea plant mushroom fruit body grows and the biological concentration of selenium element in tea plant mushroom fruit body, easy and simple to handle, easy to implement and popularization;
4. method provided by the invention not only can improve agrocybe output, agrocybe Organic Selenium, polysaccharide and ascorbic content can also be improved, an important channel is provided for human body supplements efficient, safe and reliable organic selenium source, significantly, popularizing application prospect is wide for economic benefit and social benefit.
 
Embodiment
Example 1
1. the salt of resistance to selenium is selected and to the extremely strong agrocybe cultivated strains of selenium biological concentration, prioritizing selection high yield, the high-quality simultaneously salt of resistance to selenium and be agrocybe selenium-enriching cultivation bacterial strain to the Jiangxi tea AS-1 that selenium biological concentration is extremely strong, the salt of resistance to selenium domestication is carried out to it, during domestication, domestication liquid sodium selenite aqueous solution is added in original seed and Cultivar culture medium and carry out inoculating and cultivating, preferably this domestication liquid sodium selenite aqueous solution concentration is 5 μ g/ml, original seed and cultivated species tame culture medium raw material and mass fraction is: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, add water to this domestication culture medium raw material, make its water content 60% ~ 65%, the sodium selenite mass fraction in wild Oryza species that adds water is 0.04%.
2. rich selenium intensified culturing based raw material is prepared, adding sodium selenite powder when preparing rich selenium intensified culturing based raw material is external source selenium, with natural selenium-rich raw material weed tree sawdust and wheat bran for primary raw material, add 5% corn flour, improve the content of protein in cultivation base raw material, strengthen agrocybe mycelium to the tolerance of selenium source.Wherein sodium selenite powder concentration is preferably 45mg/kg ~ 65mg/kg, is more preferably 45mg/kg.Make rich selenium intensified culturing based raw material and mass fraction is: wood chip 35%, cotton seed hulls 32%, wheat bran 25%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, add water to this rich selenium intensified culturing culture medium raw material, make its water content 60% ~ 65%, preferably 60%, after adding water, in cultivation base raw material, sodium selenite mass fraction is 0.045 ~ 0.065%, preferably 0.045%.
3. pack, sterilizing.The mixing of the raw material of rich selenium intensified culturing base is mixed thoroughly, adjustment cultivation base water content to 60%, adopt the pack of 12cm × 24cm size polyethylene normal pressure plastic sack.Carry out atmospheric steam sterilizing or high pressure steam sterilization, during normal-pressure sterilization, when autoclave temperature reaches 98 DEG C ~ 100 DEG C, keep 12h.3h ~ 4h is kept when 121 DEG C ~ 123 DEG C during autoclaving.
4. inoculate, send out bacterium.When pocket temperature after subject to sterilization drops to below 60 DEG C, be transported to by pocket in the transfer room after sterilization, require to inoculate according to sterile working, every bag cultivating kind connects 30 Bag Material bags.Postvaccinal bacterium bag is transported in culturing room to be cultivated, and culturing room's temperature controls at 22 DEG C, and once, each 30min ~ 40min, keeps indoor air relative humidity 60% ~ 70%, carry out shading cultivation in each ventilation sooner or later.
5. urge flower bud, educate mushroom.Bacterium bag is cultivated 50-60 days, and mycelia covers with bacterium bag, the high resilience of bacterium bag, and mycelia secretion pigment tells yellow water, and sack surface mycelia band point brown, now moves into mushroom room by agrocybe bacterium bag and carry out management of producing mushroom.Urge flower bud and educate the mushroom phase take " four is poor " stimulation (temperature difference, psychrometric difference, oxygen concentration is poor, illumination is poor) carry out urging flower bud and educating mushroom, promote the biological concentration of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body Organic Selenium, concrete grammar is: tell yellow water at mycelia secretion pigment, during the mycelia band point brown of sack surface, sack is stretching, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C ~ 22 DEG C, continuous 5d ~ 7d, preferred 6d widens the temperature difference, the temperature difference is made to reach 6 DEG C ~ 8 DEG C, preferably 6 DEG C, a large amount of former base is impelled to occur, budding to the sporophore growth stage and return to 22 DEG C ~ 26 DEG C, preferably 24 DEG C, impel agrocybe stem long long thick.Carry out psychrometric difference stimulation, before fruiting, every morning, relative air humidity was increased to 85% ~ 90% by 70%, is preferably increased to 85% to space spray water, cause mushroom room dry wet alternating environment, in the sporophore growth stage, directly to sack water spray, relative air humidity remains on 90% ~ 95%, preferably 90%, impel tea plant mushroom fruit body normal growth.Before gathering, 1d stops water spray.Oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min ~ 40min, preferred 30min, fruit body grow to five points ripe time, reduce ventilate, ventilate every day 1 time, ventilation time 20min.Illumination difference stimulates, after mycelium physiological ripening, intensity of illumination is increased to 500Lex ~ 800 Lex, preferred 500Lex, carry out light stimulation 5d ~ 7d, preferred 5d, until former base is formed and mushroom flower bud is formed, in the sporophore growth later stage, illumination is reduced to 200Lex ~ 500Lex, preferred 250Lex, impels stem to bleach and cap dark brown, improves agrocybe commodity value.
6. gather.Pluck when agrocybe grows to 7 ~ 8 points of maturations, to gather 4 batches ~ 5 batches agrocybes according to above-mentioned management of producing mushroom.
So namely produce the selenium enriched tea mushroom integrating idiotrophic, medical treatment, health care and Organic Selenium excellent efficacy.
Example 2
1. the salt of resistance to selenium is selected and to the extremely strong agrocybe aegerita KMFJ-FC of selenium biological concentration.Prioritizing selection Jiangxi tea AS-1 is agrocybe selenium-enriching cultivation bacterial strain, the salt of resistance to selenium domestication is carried out to it, domestication liquid sodium selenite aqueous solution is added in original seed and Cultivar culture medium and carry out inoculating and taming cultivation, original seed and Cultivar culture medium raw material and mass fraction thereof are: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, afterwards domestication culture medium raw material is added water, make its water content 60% ~ 65%, in the wild Oryza species that adds water, sodium selenite mass fraction is 0.04%.
2. rich selenium intensified culturing based raw material is prepared.When preparing rich selenium intensified culturing based raw material, adding sodium selenite powder is external source selenium, with natural selenium-rich raw material weed tree sawdust and wheat bran for primary raw material, adds 5% corn flour, improves the content of protein in cultivation base raw material, strengthen agrocybe mycelium to the tolerance of selenium source.Make rich selenium intensified culturing based raw material and mass fraction is: wood chip 35%, cotton seed hulls 32%, wheat bran 25%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, afterwards rich selenium intensified culturing culture medium raw material is added water, make cultivation base feed moisture content 65%, after adding water, in cultivation base raw material, sodium selenite mass fraction is 0.045 ~ 0.065%, preferably 0.065%.
3. pack, sterilizing.The mixing of the raw material of rich selenium intensified culturing base mixed thoroughly, adjustment moisture content in medium to 65%, adopts the packed bag of 12cm × 24cm grade polypropylene high-pressure plastic.Carry out high pressure steam sterilization, during sterilizing, when 121 DEG C ~ 123 DEG C, keep 4h.
4. inoculate, send out bacterium.When pocket temperature after subject to sterilization drops to below 60 DEG C, be transported to by pocket in the inoculating hood after sterilization, require to inoculate according to sterile working, every bag cultivating kind inoculates 40 Bag Material bags.Postvaccinal bacterium bag is transported in culturing room to be cultivated, and culturing room's temperature controls at 24 DEG C, and each ventilation sooner or later is once ventilated, each 40min, keeps indoor air relative humidity 70%, carries out shading cultivation.
5. urge flower bud, educate mushroom.Bacterium bag cultivates 60 days, and mycelia covers with bacterium bag, the high resilience of bacterium bag, and mycelia secretion pigment tells the yellow globule, sack surface mycelia band point brown.Urge flower bud and educate the mushroom phase take " four is poor " stimulation (temperature difference, psychrometric difference, oxygen concentration is poor, illumination is poor) carry out urging flower bud and educating mushroom, promote the biological concentration of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body Organic Selenium, concrete grammar is: tell yellow water at mycelia secretion pigment, during the mycelia band point brown of sack surface, sack is stretching, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C ~ 22 DEG C, continuous 7d widens the temperature difference, the temperature difference is made to reach 8 DEG C, a large amount of former base is impelled to occur, budding to the sporophore growth stage and bring up to 26 DEG C, impel agrocybe stem long long thick.Carry out psychrometric difference stimulation, before fruiting, every morning is to space spray water, relative air humidity is increased to 90% by 70%, mushroom room is built into dry wet alternating environment, in the later stage in sporophore growth stage, sprays water directly to sack, relative air humidity remains on 92% ~ 94%, impels tea plant mushroom fruit body normal growth.Before gathering, 1d stops water spray.Oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 40min, fruit body grow to five points ripe time, reduce ventilate, ventilate every day 1 time, ventilation time 20min.Illumination difference stimulates, and after mycelium physiological ripening, intensity of illumination is increased to 800 Lex, carries out light stimulation 5d, until former base is formed and buddings, in the sporophore growth later stage, illumination is reduced to 400Lex, impels stem to bleach and cap dark brown, improve agrocybe commodity value.
6. gather.Pluck when agrocybe grows to 7-8 point of maturation, 4 batches ~ 5 batches mushrooms can be gone out according to above-mentioned management of producing mushroom.
Method of the present invention, in the mode of production, take solid fermentation pure culture planting type, carry out the rich selenium of agrocybe to cultivate, " four is poor " stimulus method (temperature difference, psychrometric difference, oxygen concentration are poor, illumination is poor) is taked to carry out urging flower bud and educate mushroom urging flower bud and educate the mushroom phase, promote the biological concentration of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body Organic Selenium.This method not only can improve Organic Selenium in agrocybe, polysaccharide and ascorbic content, agrocybe output can also be improved, agrocybe selenium content is up to 225.80mg/kg, agrocybe biological transformation ratio reaches 100 ~ 110%, provides an important channel for human body supplements efficient, safe and reliable Organic Selenium.Meanwhile, the method is easy and simple to handle, easy to implement, Enriching Selenium Be very effective, and culturing raw material is sufficient, with low cost, and area under cultivation is extensive, and social and economic benefits is remarkable, has a extensive future.
Describe several embodiment of the present invention and advantage thereof by means of example above, describe comparatively concrete and detailed, it should be noted that and do not think that description forms limiting the scope of the invention above.It should be pointed out that to those skilled in the art, when not departing from the present invention's design, multiple changes and improvements can be made to above-mentioned embodiment, thinking that this changes and improvements all belong to protection scope of the present invention.Protection scope of the present invention is only defined by the following claims.

Claims (3)

1. a method for selenium enriched tea mushroom cultivation, is characterized in that being realized by following steps: first select the salt of resistance to selenium and to the extremely strong agrocybe aegerita KMFJ-FC of selenium biological concentration, and carries out the salt of resistance to selenium domestication to it; Then prepare agrocybe rich selenium intensified culturing based raw material, by the pack of cultivation base raw material, sterilizing, inoculation, cultivate agrocybe mycelium; After agrocybe mycelium physiological ripening, agrocybe bacterium bag is moved into mushroom room and carries out management of producing mushroom, undertaken urging flower bud and educating mushroom by regulation and control agrocybe growth temperature, humidity, ventilation and illumination condition, promote growing and biological concentration to selenium of agrocybe, final production goes out to integrate the selenium enriched tea mushroom of agrocybe idiotrophic, medical treatment, health care and Organic Selenium excellent efficacy.
2. the method for a kind of selenium enriched tea mushroom cultivation according to claim 1, wherein, select the salt of resistance to selenium and the cultivated strains Jiangxi tea AS-1 strong to selenium bioaccumulation ability, and the salt of resistance to selenium domestication is carried out to it, when taming, domestication liquid sodium selenite aqueous solution is added in original seed and cultivated species domestication medium and carry out inoculating and cultivating, original seed and cultivated species tame culture medium raw material and mass fraction is: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, add water to it and make its water content 60% ~ 65%, the sodium selenite mass fraction in wild Oryza species that adds water is 0.04%.
3. the method for a kind of selenium enriched tea mushroom cultivation according to claim 1, when preparing rich selenium intensified culturing based raw material, adding sodium selenite powder is external source selenium, with natural selenium-rich raw material weed tree sawdust and wheat bran for primary raw material, add 5% corn flour, improve the content of protein in cultivation base raw material, strengthen agrocybe mycelium to the tolerance of selenium source, wherein rich selenium intensified culturing based raw material and mass fraction thereof are: wood chip 35%, wheat bran 25%, cotton seed hulls 32%, corn flour 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, add water to it and make its water content 60% ~ 65%, after adding water, in cultivation base raw material, sodium selenite mass fraction is 0.045 ~ 0.065%,
The method of a kind of selenium enriched tea mushroom cultivation according to claim 1, take " four is poor " to stimulate to carry out urging flower bud and educate mushroom urging flower bud and educate the mushroom phase, promote the biological concentration of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body Organic Selenium, this four difference is for temperature difference, psychrometric difference, oxygen concentration is poor, illumination is poor, and concrete management of producing mushroom method is:
After mycelia is covered with bag and starts to tell the yellow globule, sack is stretching, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C ~ 22 DEG C, continuous 5d ~ 7d widens day and night temperature, the temperature difference is made to reach 6 DEG C ~ 8 DEG C, impel a large amount of former base to occur, temperature is brought up to 22 DEG C ~ 26 DEG C to the sporophore growth stage after buddingging, impel agrocybe stem long long thick; Psychrometric difference stimulates, before fruiting, every morning is to space spray water, relative air humidity brings up to 85% ~ 90% by 70%, build mushroom room dry wet alternating environment, in the sporophore growth stage, directly to sack water spray, relative air humidity remains on 90% ~ 95%, impel tea plant mushroom fruit body normal growth, before gathering, 1d stops water spray; Oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min ~ 40min, fruit body grow to five points ripe time, ventilate every day 1 time, ventilation time 20min; Illumination difference stimulates, after mycelium physiological ripening, intensity of illumination is increased to 500Lex ~ 800 Lex, light stimulation 5d ~ 7d, until former base is formed and mushroom flower bud is formed, in the sporophore growth later stage, intensity of illumination is reduced to 200Lex ~ 500Lex, impel stem to bleach and cap dark brown, improve agrocybe commodity value;
Pluck when agrocybe grows to 7-8 point of maturation, 4 batches ~ 5 batches mushrooms can be gone out according to above-mentioned management of producing mushroom.
CN201410501834.0A 2014-09-27 2014-09-27 The method of a kind of selenium enriched tea mushroom cultivation Active CN104303825B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410501834.0A CN104303825B (en) 2014-09-27 2014-09-27 The method of a kind of selenium enriched tea mushroom cultivation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410501834.0A CN104303825B (en) 2014-09-27 2014-09-27 The method of a kind of selenium enriched tea mushroom cultivation

Publications (2)

Publication Number Publication Date
CN104303825A true CN104303825A (en) 2015-01-28
CN104303825B CN104303825B (en) 2016-06-01

Family

ID=52359266

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410501834.0A Active CN104303825B (en) 2014-09-27 2014-09-27 The method of a kind of selenium enriched tea mushroom cultivation

Country Status (1)

Country Link
CN (1) CN104303825B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104770214A (en) * 2015-03-22 2015-07-15 邬金飞 Agrocybe cylindracea cultivation method
CN104770215A (en) * 2015-03-22 2015-07-15 邬金飞 Agrocybe cylindracea cultivation method
CN106472106A (en) * 2016-09-30 2017-03-08 桂林淮安天然保健品开发有限公司 A kind of ganoderma lucidum cultivation method improving ganoderma polyoses content
CN106748231A (en) * 2016-12-30 2017-05-31 广西壮族自治区农业科学院微生物研究所 A kind of selenium enriched oyster mushroom culture medium and application method containing Moringa bits
CN112997805A (en) * 2021-03-31 2021-06-22 广东雄达实业发展有限公司 Agrocybe cylindracea cultivation method taking stem raw material of camellia fruit tree as culture medium
CN113348961A (en) * 2021-03-31 2021-09-07 广东雄达实业发展有限公司 Agrocybe cylindracea cultivation method taking camellia fruit shell raw material as culture medium

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060091840A (en) * 2005-02-16 2006-08-22 김승 Process for cultivating agrocybe cylindracea containing minerals and fertilizer containing the by-product thereof
CN101182266A (en) * 2007-10-31 2008-05-21 杭州农发科技开发有限公司 Method for planting selenium-enriched pleurotus eryngii and culture medium thereof
CN101407798A (en) * 2008-11-01 2009-04-15 甘肃省科学院生物研究所 Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material
CN101496484A (en) * 2008-01-31 2009-08-05 马俊生 Method for domesticating and cultivating selenium-rich mushroom strain
CN101755615A (en) * 2009-12-31 2010-06-30 张寿禄 Cultivation method of high trace element edible and medical fungi
CN102047811A (en) * 2009-10-30 2011-05-11 周玲 High-quality and high-yield culturing method of agrocybe aegerita
CN102511305A (en) * 2011-12-07 2012-06-27 苏州硒谷科技有限公司 Method for cultivating high-selenium-tolerability strain of edible fungi
CN102771314A (en) * 2012-08-15 2012-11-14 苏州硒谷科技有限公司 Method for culturing selenium-enriched cordyceps militaris
CN103975763A (en) * 2014-05-14 2014-08-13 河北沃达生物技术有限公司 Agrocybe cylindracea cultivation method

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060091840A (en) * 2005-02-16 2006-08-22 김승 Process for cultivating agrocybe cylindracea containing minerals and fertilizer containing the by-product thereof
CN101182266A (en) * 2007-10-31 2008-05-21 杭州农发科技开发有限公司 Method for planting selenium-enriched pleurotus eryngii and culture medium thereof
CN101496484A (en) * 2008-01-31 2009-08-05 马俊生 Method for domesticating and cultivating selenium-rich mushroom strain
CN101407798A (en) * 2008-11-01 2009-04-15 甘肃省科学院生物研究所 Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material
CN102047811A (en) * 2009-10-30 2011-05-11 周玲 High-quality and high-yield culturing method of agrocybe aegerita
CN101755615A (en) * 2009-12-31 2010-06-30 张寿禄 Cultivation method of high trace element edible and medical fungi
CN102511305A (en) * 2011-12-07 2012-06-27 苏州硒谷科技有限公司 Method for cultivating high-selenium-tolerability strain of edible fungi
CN102771314A (en) * 2012-08-15 2012-11-14 苏州硒谷科技有限公司 Method for culturing selenium-enriched cordyceps militaris
CN103975763A (en) * 2014-05-14 2014-08-13 河北沃达生物技术有限公司 Agrocybe cylindracea cultivation method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104770214A (en) * 2015-03-22 2015-07-15 邬金飞 Agrocybe cylindracea cultivation method
CN104770215A (en) * 2015-03-22 2015-07-15 邬金飞 Agrocybe cylindracea cultivation method
CN106472106A (en) * 2016-09-30 2017-03-08 桂林淮安天然保健品开发有限公司 A kind of ganoderma lucidum cultivation method improving ganoderma polyoses content
CN106748231A (en) * 2016-12-30 2017-05-31 广西壮族自治区农业科学院微生物研究所 A kind of selenium enriched oyster mushroom culture medium and application method containing Moringa bits
CN112997805A (en) * 2021-03-31 2021-06-22 广东雄达实业发展有限公司 Agrocybe cylindracea cultivation method taking stem raw material of camellia fruit tree as culture medium
CN113348961A (en) * 2021-03-31 2021-09-07 广东雄达实业发展有限公司 Agrocybe cylindracea cultivation method taking camellia fruit shell raw material as culture medium

Also Published As

Publication number Publication date
CN104303825B (en) 2016-06-01

Similar Documents

Publication Publication Date Title
CN102786333B (en) Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same
CN104041330B (en) Ganoderma tsugae imitates wild juggle cultivation method
CN102187787B (en) Method for culturing rare edible fungi including tricholoma lobayense heim and clitocybe maxima by using vine shoot dust
CN101444170B (en) Strain separation method of apricot ormer mushroom and cultivating method thereof
CN103918475B (en) The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom
CN103891524B (en) The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma
CN106258483B (en) Morchella multilayer base material planting method in high-cold high-altitude area
CN102630481B (en) Cultivation method for oospore oudemansiella mucida
CN104303825B (en) The method of a kind of selenium enriched tea mushroom cultivation
CN103907478B (en) The method that flat mushroom bonsai type is cultivated and the substratum for cultivating white mushroom
CN102960184A (en) Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof
CN102668878A (en) Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium
CN105940960B (en) A kind of soil covering culture method of interplanting ganoderma lucidum in tea gardens
CN103583225A (en) Method for cultivating good-quality high-yield pleurotus geesteranus by means of cassava stems
CN108812053A (en) A kind of culture medium and cultural method of indoor growing selenium-rich Stropharia rugoso-annulata
CN104823703A (en) Culture method for pleurotus nebrodensis
CN106358755A (en) Interplanting cultivation method of lentinula edodes, morchella vulgarises and kudzuvine roots
CN107347461A (en) A kind of method of comprehensive utilization of bamboo resource
CN103907477A (en) Method and medium for cultivating mushrooms in potted landscape manner
CN105493894A (en) Cultivation method for implanting mulberry trees with straw mushroom
CN104641943A (en) Method for cultivating pleurotu comucopiae on mulberry twigs
CN105009940A (en) Method for culturing enoki mushroom with herb residues
CN101411289B (en) Method for cultivating gold needle mushroom using ramie core
CN104472219A (en) Agrocybe cylindracea cultivation method
CN102835246A (en) Cultivation method for producing coprinus comatus by using mushroom residue and biogas residue mixture

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20170327

Address after: Jade Lake Road 330200 Jiangxi city of Nanchang province Nanchang County Blue Economic Development Zone No. 487

Patentee after: JIANGXI ZHENGWEI FOOD CO.,LTD.

Address before: 330200 Jiangxi province Qingyunpu District of Nanchang City Nan Road No. 602

Patentee before: RESEARCH INSTITUTE OF AGRICULTURAL APPLICATION OF MICROORGANISMS OF JIANGXI ACADEMY OF AGRICULTURAL SCIENCES

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20190422

Address after: 344900 Guangchang County Industrial Park, Fuzhou City, Jiangxi Province

Patentee after: Guangchang Zhenglian Biotechnology Co.,Ltd.

Address before: 330200 Yuhu Road 487, Xiaolan Economic Development Zone, Nanchang County, Nanchang City, Jiangxi Province

Patentee before: JIANGXI ZHENGWEI FOOD CO.,LTD.

PE01 Entry into force of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: A method for cultivating selenium rich tea tree mushroom

Effective date of registration: 20231211

Granted publication date: 20160601

Pledgee: Jiangxi Guangchang Rural Commercial Bank Co.,Ltd.

Pledgor: Guangchang Zhenglian Biotechnology Co.,Ltd.

Registration number: Y2023980070822