CN104303825B - The method of a kind of selenium enriched tea mushroom cultivation - Google Patents
The method of a kind of selenium enriched tea mushroom cultivation Download PDFInfo
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- CN104303825B CN104303825B CN201410501834.0A CN201410501834A CN104303825B CN 104303825 B CN104303825 B CN 104303825B CN 201410501834 A CN201410501834 A CN 201410501834A CN 104303825 B CN104303825 B CN 104303825B
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 137
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 115
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 115
- 239000011669 selenium Substances 0.000 title claims abstract description 115
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241001122767 Theaceae Species 0.000 title 1
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- 240000003553 Leptospermum scoparium Species 0.000 claims abstract description 59
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 59
- 239000002994 raw material Substances 0.000 claims abstract description 45
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- 235000008121 Agrocybe aegerita Nutrition 0.000 claims abstract description 4
- 244000045069 Agrocybe aegerita Species 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 230000033228 biological regulation Effects 0.000 claims abstract description 3
- 229940091258 selenium supplement Drugs 0.000 claims description 105
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 19
- 229960001471 sodium selenite Drugs 0.000 claims description 19
- 235000015921 sodium selenite Nutrition 0.000 claims description 19
- 239000011781 sodium selenite Substances 0.000 claims description 19
- 235000006468 Thea sinensis Nutrition 0.000 claims description 18
- 235000013399 edible fruits Nutrition 0.000 claims description 18
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- 102000004169 proteins and genes Human genes 0.000 claims description 5
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
A method for selenium enriched tea mushroom cultivation, comprising: first select the salt of resistance to selenium and the agrocybe aegerita KMFJ-FC that selenium biomagnification is extremely strong, and it carries out the salt of resistance to selenium and rich selenium domestication; Then prepare the rich selenium intensified culturing based raw material of tea tree mushroom, by the pack of cultivation base raw material, sterilizing, inoculation, cultivate tea tree mushroom mycelium; After tea tree mushroom mycelium physiological maturity, tea tree mushroom bacterium bag is moved into out mushroom room and carries out management of producing mushroom, undertaken urging flower bud and educating mushroom by regulation and control tea tree mushroom growth temperature, humidity, ventilation and illumination condition, promote tea tree mushroom grow and to the biomagnification of selenium, final production goes out to integrate the selenium enriched tea mushroom of tea tree mushroom self nutrition, medical treatment, health care and organoselenium excellent efficacy. The method is easy and simple to handle, is easy to implement, and enrichment selenium effect is remarkable, and culturing raw material is sufficient, with low cost, and cultivated area is extensive, and social and economic benefits is remarkable, has a extensive future.
Description
Technical field
The invention belongs to bacterium industry field, it is specifically related to the method for a kind of selenium enriched tea mushroom cultivation.
Background technology
Tea tree mushroom delicious flavour, nutritious, there is higher edible and pharmaceutical use. Selenium is a kind of trace element necessary in humans and animals body, human body is had much important effect, the shortage of selenium can directly cause Keshan disease and Kaschin-Beck disease, the extensive Problems existing in the soil Que Xishi world, the 72% of China's area belongs to scarce selenium and low selenium area, the process of growth of the farm crop in these areas cannot obtain sufficient selenium from soil, thus form the generally scarce selenium state of food chain. Selenium-enriched food of ingesting has become the important means ensureing national health at present. But the toxicity of inorganic selenium is far longer than organoselenium, edible mushrooms is one of best rich selenium carrier, a large amount of research and practice proves, selenium is had extremely strong inrichment by tea tree mushroom, by it is carried out selenium nutrition-intensified, the a large amount of enrichment in tea plant mushroom fruit body of selenium element can be made to close bioconversion, it is to increase the content of tea plant mushroom fruit body organoselenium.
The object of present method is to provide the cultivating method of a kind of selenium enriched tea mushroom, produce the selenium enriched tea mushroom integrating tea tree mushroom self nutrition, medical treatment, health care and organoselenium excellent efficacy, there is provided an important channel for human body supplements organoselenium efficient, safe and reliable, and produce significant economic benefit and social benefit.
Summary of the invention
Method object provided by the invention is a kind of method providing selenium enriched tea mushroom to cultivate, take tea tree mushroom as carrier, select salt tolerant and the strong cultivated strain of selenium enriching functions, and it is carried out the salt of resistance to selenium and rich selenium domestication, add external source selenium, the rich selenium intensified culturing matrix of preparation, comprehensive regulation temperature, humidity, ventilation, the tea tree mushroom growth conditionss such as illumination, promote the biomagnification of selenium element in tea plant mushroom fruit body, improve the content of organoselenium in tea tree mushroom, produce collection tea tree mushroom self nutrition, medical treatment, the selenium enriched tea mushroom that health care and organoselenium excellent efficacy are integrated, for human body supplements efficient, safety, reliable organoselenium provides an important channel.
For achieving the above object, the present invention takes following technical scheme:
The method of a kind of selenium enriched tea mushroom cultivation, first select the salt of resistance to selenium ability and to the strong agrocybe aegerita KMFJ-FC of selenium biomagnification and it is carried out the domestication of the salt of resistance to selenium and selenium rich ability, then the rich selenium hot housing base of tea tree mushroom is prepared, substratum is packed, sterilizing, inoculation, cultivate tea tree mushroom mycelium, after tea tree mushroom mycelium physiological maturity, tea tree mushroom bacterium bag is moved into out mushroom room and carries out management of producing mushroom, pass through regulating and controlling temperature, humidity, the tea tree mushroom growth conditionss such as ventilation and illumination, carry out tea tree mushroom and urge flower bud, educate mushroom, promote tea tree mushroom growing to the enrichment of selenium and sporophore, final production goes out to collect tea tree mushroom self nutrition, medical treatment, the selenium enriched tea mushroom that health care and organoselenium excellent efficacy are integrated.
Its concrete steps are as follows:
1. the salt of resistance to selenium ability and the selenium enriched tea mushroom cultivated strain that selenium biomagnification is strong is selected. Jiangxi tea AS-1 is selected to be tea tree mushroom selenium-enriching cultivation bacterial strain, and it is carried out the domestication of the salt of resistance to selenium and selenium rich ability, during domestication, domestication liquid sodium selenite aqueous solution is added in original seed and cultivar domestication substratum and carry out inoculating and cultivating, original seed and Cultivar culture medium raw material and massfraction thereof be: wood chip 35%, wheat bran 25%, cotton seed hulls 33%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, and add water to it and make its water content 60%��65%, in the wild Oryza species that adds water, Sodium Selenite massfraction is 0.04%;
2. the rich selenium intensified culturing based raw material of preparation. When preparing rich selenium intensified culturing based raw material, adding Sodium Selenite powder is external source selenium, taking natural selenium-rich raw material weed tree sawdust and wheat bran as main raw material, adds 5% Semen Maydis powder, improve the content of protein in cultivation base raw material, strengthen tea tree mushroom mycelium to the tolerance in selenium source. Rich selenium intensified culturing based raw material and massfraction thereof be: wood chip 35%, wheat bran 25%, cotton seed hulls 32%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, adding water to it and make its water content 60%��65%, after adding water, in cultivation base raw material, Sodium Selenite massfraction is 0.045��0.065%;
3. pack, sterilizing. The raw material mixing of rich selenium intensified culturing base being mixed thoroughly, adjustment moisture content in medium to 60%��65%, adopts 12cm �� 24cm size polyethylene normal pressure plastics bag or the packed bag of polypropylene high-pressure plastic. Carry out atmospheric steam sterilizing or high pressure steam sterilization. During atmospheric steam sterilizing, when Autoclave temperature reaches 98 DEG C��100 DEG C, keep 12h��14h, during high pressure steam sterilization, when 121 DEG C��123 DEG C, keep 3h��4h.
4. inoculate, send out bacterium. Time below material bag temperature decrease to 60 DEG C after subject to sterilization, material bag being transported to the inoculation room after sterilization or in inoculation tank, requires to inoculate according to aseptic technique, every bag cultivating kind connects 30 bags��40 Bag Material bags. Bacterium bag after inoculation is transported in culturing room to cultivate, and culturing room's temperature controls at about 22 DEG C, and once, each 30min��40min, keeps indoor air relative humidity 60%��70%, carry out shading cultivation in each ventilation sooner or later.
5. urge flower bud, educate mushroom. Bacterium bag cultivates 50 60 days, mycelia covers with bacterium bag, bacterium bag high resilience, mycelia secretion pigment tells yellow water, during bag discharge surface mycelia band point brown, take " four is poor " stimulus method (temperature head, psychrometric difference, oxygen concentration are poor, illumination is poor) to carry out urging flower bud and educating mushroom, promote the biomagnification of selenium element in tea plant mushroom fruit body.
Concrete grammar is:
After mycelia is covered with bag and starts to tell the yellow globule, bag mouth is stretched, remove the old bacterium block of inoculation, carrying out thermal stimulation, reduce the temperature to 14 DEG C��22 DEG C, continuous 5d��7d widens day and night temperature, the temperature difference is made to reach 6 DEG C��8 DEG C, impel a large amount of former base to occur, temperature is brought up to 22 DEG C��26 DEG C to the sporophore growth stage after buddingging, impel tea tree mushroom stem length length length thick. Psychrometric difference stimulates, before going out mushroom, every morning is to space spray water, relative air humidity brings up to 85%��90% by 70%, build mushroom room dry wet alternating environment, in the sporophore growth stage, directly to bag mouth water spray, relative air humidity remains on 90%��95%, impelling tea plant mushroom fruit body normal growth, before gathering, 1d stops water spray. Oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min��40min, sporophore grow to five points ripe time, ventilate every day 1 time, ventilation time 20min. Illumination difference stimulates, and after mycelium physiological maturity, intensity of illumination is increased to 500Lex��800Lex, light stimulation 5d��7d, until former base is formed and buddings. In the sporophore growth later stage, intensity of illumination is reduced to 200Lex��500Lex, impels stem to become white and cap Vandyke brown, it is to increase tea tree mushroom commodity value.
6. gather. Pluck when tea tree mushroom grows to when 7-8 divides maturation, pluck 4 batches��5 batches mushrooms according to above-mentioned management of producing mushroom method.
The remarkable advantage of the present invention is:
1. filtering out the salt of resistance to selenium and be cultivated strain to the strong Jiangxi tea AS-1 of selenium bioaccumulation ability, and it carries out the salt of resistance to selenium and rich selenium domestication, it is to increase the cultivated strain salt of resistance to selenium ability and to selenium bioconcentration, concentration effect is remarkable;
2. adding commercially available Sodium Selenite powder is external source selenium, taking natural selenium-rich raw material weedtree wood chip and wheat bran as selenium source carrier, improve weedtree wood chip and the wheat bran content at culture medium for cultivating, improve the content of protein in culture medium for cultivating, improve tea tree mushroom mycelia to selenium source tolerance, the rich selenium intensified culturing matrix of preparation, starting material are extensive, with low cost;
3. urging flower bud and educating the mushroom phase, " four is poor " stimulus method (temperature head, psychrometric difference, oxygen concentration are poor, illumination is poor) is taked to carry out urging flower bud and educating mushroom, promote that tea plant mushroom fruit body grows and the biomagnification of selenium element in tea plant mushroom fruit body, easy and simple to handle, it is easy to implement and promote;
4. method provided by the invention is possible not only to improve tea tree mushroom output, tea tree mushroom organoselenium, polysaccharide and ascorbic content can also be improved, an important channel is provided for human body supplements organoselenium source efficient, safe and reliable, economic benefit and social benefit are remarkable, and popularizing application prospect is wide.
Embodiment
Example 1
1. the salt of resistance to selenium and the tea tree mushroom cultivated strain that selenium biomagnification is extremely strong is selected, prioritizing selection high yield, the high-quality salt of resistance to selenium and be tea tree mushroom selenium-enriching cultivation bacterial strain to the Jiangxi tea AS-1 that selenium biomagnification is extremely strong simultaneously, it is carried out the salt of resistance to selenium domestication, during domestication, domestication liquid sodium selenite aqueous solution is added in original seed and Cultivar culture medium and carry out inoculating and cultivating, preferably this domestication liquid sodium selenite aqueous solution concentration is 5 �� g/ml, original seed and cultivar tame culture medium raw material and massfraction is: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, add water to this domestication culture medium raw material, make its water content 60%��65%, the Sodium Selenite massfraction in wild Oryza species that adds water is 0.04%.
2. the rich selenium intensified culturing based raw material of preparation, adding Sodium Selenite powder during preparation richness selenium intensified culturing based raw material is external source selenium, taking natural selenium-rich raw material weed tree sawdust and wheat bran as main raw material, add 5% Semen Maydis powder, improve the content of protein in cultivation base raw material, strengthen tea tree mushroom mycelium to the tolerance in selenium source. Wherein Sodium Selenite powder concentration is preferably 45mg/kg��65mg/kg, it is more preferable to be 45mg/kg. Make rich selenium intensified culturing based raw material and massfraction is: wood chip 35%, cotton seed hulls 32%, wheat bran 25%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, add water to this richness selenium intensified culturing culture medium raw material, make its water content 60%��65%, preferably 60%, after adding water, in cultivation base raw material, Sodium Selenite massfraction is 0.045��0.065%, it is preferable that 0.045%.
3. pack, sterilizing. The raw material mixing of rich selenium intensified culturing base is mixed thoroughly, adjustment cultivation base water content to 60%, adopt the pack of 12cm �� 24cm size polyethylene normal pressure plastics bag. Carry out atmospheric steam sterilizing or high pressure steam sterilization, during normal-pressure sterilization, when Autoclave temperature reaches 98 DEG C��100 DEG C, keep 12h. 3h��4h is kept when 121 DEG C��123 DEG C during autoclaving.
4. inoculate, send out bacterium. Time below material bag temperature decrease to 60 DEG C after subject to sterilization, being transported to by material bag in the inoculation room after sterilization, require to inoculate according to aseptic technique, every bag cultivating kind connects 30 Bag Material bags. Bacterium bag after inoculation is transported in culturing room to cultivate, and culturing room's temperature controls at 22 DEG C, and once, each 30min��40min, keeps indoor air relative humidity 60%��70%, carry out shading cultivation in each ventilation sooner or later.
5. urge flower bud, educate mushroom. bacterium bag cultivates 50 60 days, and mycelia covers with bacterium bag, bacterium bag high resilience, and mycelia secretion pigment tells yellow water, and bag discharge surface mycelia band point brown, now moves into out mushroom room by tea tree mushroom bacterium bag and carry out management of producing mushroom. urge flower bud and educate the mushroom phase take " four is poor " stimulation (temperature head, psychrometric difference, oxygen concentration is poor, illumination is poor) carry out urging flower bud and educating mushroom, promote the biomagnification of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body organoselenium, concrete grammar is: secretes pigment mycelia and tells yellow water, during bag discharge surface mycelia band point brown, bag mouth is stretched, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C��22 DEG C, continuous 5d��7d, preferred 6d widens the temperature difference, the temperature difference is made to reach 6 DEG C��8 DEG C, preferably 6 DEG C, a large amount of former base is impelled to occur, budding to the sporophore growth stage and return to 22 DEG C��26 DEG C, preferably 24 DEG C, impel tea tree mushroom stem length length length thick. carry out psychrometric difference stimulation, before going out mushroom, every morning is increased to 85%��90% to space spray water, relative air humidity by 70%, it is preferable that be increased to 85%, cause out mushroom room dry wet alternating environment, in the sporophore growth stage, directly to bag mouth water spray, relative air humidity remains on 90%��95%, preferably 90%, impel tea plant mushroom fruit body normal growth. before gathering, 1d stops water spray. oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min��40min, it is preferable that 30min, sporophore grow to five points ripe time, reduce ventilate, ventilate every day 1 time, ventilation time 20min. illumination difference stimulates, after mycelium physiological maturity, intensity of illumination is increased to 500Lex��800Lex, preferred 500Lex, carry out light stimulation 5d��7d, it is preferable that 5d, until former base is formed and mushroom flower bud is formed, in the sporophore growth later stage, illumination is reduced to 200Lex��500Lex, preferred 250Lex, impels stem to become white and cap Vandyke brown, it is to increase tea tree mushroom commodity value.
6. gather. Pluck when tea tree mushroom grows to 7��8 points of maturations, gather 4 batches��5 batches tea tree mushroom according to above-mentioned management of producing mushroom.
So namely produce the selenium enriched tea mushroom integrating self nutrition, medical treatment, health care and organoselenium excellent efficacy.
Example 2
1. the salt of resistance to selenium and the agrocybe aegerita KMFJ-FC that selenium biomagnification is extremely strong is selected. Prioritizing selection Jiangxi tea AS-1 is tea tree mushroom selenium-enriching cultivation bacterial strain, it is carried out the salt of resistance to selenium domestication, domestication liquid sodium selenite aqueous solution is added in original seed and Cultivar culture medium and carry out inoculating and taming cultivation, original seed and Cultivar culture medium raw material and massfraction thereof be: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, afterwards domestication culture medium raw material is added water, making its water content 60%��65%, in the wild Oryza species that adds water, Sodium Selenite massfraction is 0.04%.
2. the rich selenium intensified culturing based raw material of preparation. During the rich selenium intensified culturing based raw material of preparation, adding Sodium Selenite powder is external source selenium, taking natural selenium-rich raw material weed tree sawdust and wheat bran as main raw material, adds 5% Semen Maydis powder, it is to increase the content of protein in cultivation base raw material, strengthens tea tree mushroom mycelium to the tolerance in selenium source. Make rich selenium intensified culturing based raw material and massfraction is: wood chip 35%, cotton seed hulls 32%, wheat bran 25%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, afterwards rich selenium intensified culturing culture medium raw material is added water, make cultivation base feed moisture content 65%, after adding water, in cultivation base raw material, Sodium Selenite massfraction is 0.045��0.065%, it is preferable that 0.065%.
3. pack, sterilizing. The raw material mixing of rich selenium intensified culturing base being mixed thoroughly, adjustment moisture content in medium to 65%, adopts 12cm �� 24cm specification packed bag of polypropylene high-pressure plastic. Carry out high pressure steam sterilization, during sterilizing, when 121 DEG C��123 DEG C, keep 4h.
4. inoculate, send out bacterium. Time below material bag temperature decrease to 60 DEG C after subject to sterilization, being transported to by material bag in the inoculation tank after sterilization, require to inoculate according to aseptic technique, every bag cultivating kind inoculates 40 Bag Material bags. Bacterium bag after inoculation is transported in culturing room to cultivate, and culturing room's temperature controls at 24 DEG C, and each ventilation sooner or later is once ventilated, each 40min, keeps indoor air relative humidity 70%, carries out shading cultivation.
5. urge flower bud, educate mushroom. bacterium bag cultivates 60 days, and mycelia covers with bacterium bag, bacterium bag high resilience, and mycelia secretion pigment tells the yellow globule, bag discharge surface mycelia band point brown. urge flower bud and educate the mushroom phase take " four is poor " stimulation (temperature head, psychrometric difference, oxygen concentration is poor, illumination is poor) carry out urging flower bud and educating mushroom, promote the biomagnification of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body organoselenium, concrete grammar is: secretes pigment mycelia and tells yellow water, during bag discharge surface mycelia band point brown, bag mouth is stretched, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C��22 DEG C, continuous 7d widens the temperature difference, the temperature difference is made to reach 8 DEG C, a large amount of former base is impelled to occur, budding to the sporophore growth stage and bring up to 26 DEG C, impel tea tree mushroom stem length length length thick. carry out psychrometric difference stimulation, before going out mushroom, every morning is to space spray water, relative air humidity is increased to 90% by 70%, build into dry wet alternating environment going out mushroom room, in the later stage in sporophore growth stage, spray water directly to bag mouth, relative air humidity remains on 92%��94%, impels tea plant mushroom fruit body normal growth. before gathering, 1d stops water spray. oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 40min, sporophore grow to five points ripe time, reduce ventilate, ventilate every day 1 time, ventilation time 20min. illumination difference stimulates, and after mycelium physiological maturity, intensity of illumination is increased to 800Lex, carries out light stimulation 5d, until former base is formed and buddings, in the sporophore growth later stage, illumination is reduced to 400Lex, impels stem to become white and cap Vandyke brown, it is to increase tea tree mushroom commodity value.
6. gather. Pluck when tea tree mushroom grows to when 7-8 divides maturation, 4 batches��5 batches mushrooms can be gone out according to above-mentioned management of producing mushroom.
The method of the present invention, in the mode of production, take solid fermentation pure culture planting type, carry out the rich selenium of tea tree mushroom to cultivate, " four is poor " stimulus method (temperature head, psychrometric difference, oxygen concentration are poor, illumination is poor) is taked to carry out urging flower bud and educate mushroom urging flower bud and educate the mushroom phase, promote the biomagnification of selenium element in tea plant mushroom fruit body, it is to increase the content of tea plant mushroom fruit body organoselenium. Present method is possible not only to improve organoselenium, polysaccharide and ascorbic content in tea tree mushroom, tea tree mushroom output can also be improved, tea tree mushroom selenium content is up to 225.80mg/kg, tea tree mushroom biological transformation ratio reaches 100��110%, provides an important channel for human body supplements organoselenium efficient, safe and reliable. Meanwhile, the method is easy and simple to handle, is easy to implement, and enrichment selenium effect is remarkable, and culturing raw material is sufficient, with low cost, and cultivated area is extensive, and social and economic benefits is remarkable, has a extensive future.
Describe several enforcement mode and the advantage thereof of the present invention above by means of example, describe comparatively concrete and detailed, it should be noted that do not think that description forms limiting the scope of the invention above. It should be appreciated that to those skilled in the art, when not departing from present inventive concept, above-mentioned enforcement mode can be made multiple changes and improvements, it is believed that this kind of changes and improvements all belong to protection scope of the present invention. Protection scope of the present invention is only defined by the following claims.
Claims (2)
1. the method for a selenium enriched tea mushroom cultivation, it is characterised in that realized by following step: first select the salt of resistance to selenium and the agrocybe aegerita KMFJ-FC that selenium biomagnification is extremely strong, and it is carried out the salt of resistance to selenium domestication, then prepare the rich selenium intensified culturing based raw material of tea tree mushroom, by the pack of cultivation base raw material, sterilizing, inoculation, cultivate tea tree mushroom mycelium, after tea tree mushroom mycelium physiological maturity, tea tree mushroom bacterium bag is moved into out mushroom room and carries out management of producing mushroom, by regulation and control tea tree mushroom growth temperature, humidity, ventilation and illumination condition carry out urging flower bud and educating mushroom, promote tea tree mushroom grow and to the biomagnification of selenium, final production goes out to collect tea tree mushroom self nutrition, medical treatment, the selenium enriched tea mushroom that health care and organoselenium excellent efficacy are integrated, wherein when preparing rich selenium intensified culturing based raw material, adding Sodium Selenite powder is external source selenium, taking natural selenium-rich raw material weed tree sawdust and wheat bran as main raw material, add 5% Semen Maydis powder, improve the content of protein in cultivation base raw material, strengthen tea tree mushroom mycelium to the tolerance in selenium source, wherein rich selenium intensified culturing based raw material and massfraction thereof are: wood chip 35%, wheat bran 25%, cotton seed hulls 32%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, lime 1%, add water to it and make its water content 60%��65%, after adding water, in cultivation base raw material, Sodium Selenite massfraction is 0.045��0.065%,
Take " four is poor " to stimulate to carry out urging flower bud and educate mushroom urging flower bud and educate the mushroom phase, promote the biomagnification of selenium element in tea plant mushroom fruit body, improve the content of tea plant mushroom fruit body organoselenium, this four difference is temperature head, psychrometric difference, oxygen concentration is poor, illumination is poor, concrete management of producing mushroom method is: after mycelia is covered with bag and starts to tell the yellow globule, bag mouth is stretched, remove the old bacterium block of inoculation, carry out thermal stimulation, reduce the temperature to 14 DEG C��22 DEG C, continuous 5d��7d widens day and night temperature, the temperature difference is made to reach 6 DEG C��8 DEG C, a large amount of former base is impelled to occur, temperature is brought up to 22 DEG C��26 DEG C to the sporophore growth stage after buddingging, tea tree mushroom stem is impelled to look longer thicker, psychrometric difference stimulates, before going out mushroom, every morning is to space spray water, relative air humidity brings up to 85%��90% by 70%, build mushroom room dry wet alternating environment, in the sporophore growth stage, directly to bag mouth water spray, relative air humidity remains on 90%��95%, impelling tea plant mushroom fruit body normal growth, before gathering, 1d stops water spray, oxygen concentration difference stimulate, after opening bag, every day ventilates 3 times, early, middle and late each 1 time, each 30min��40min, sporophore grow to five points ripe time, ventilate every day 1 time, ventilation time 20min, illumination difference stimulates, after mycelium physiological maturity, intensity of illumination is increased to 500Lex��800Lex, light stimulation 5d��7d, until former base is formed and mushroom flower bud is formed, in the sporophore growth later stage, intensity of illumination is reduced to 200Lex��500Lex, stem is impelled to become white and cap Vandyke brown, it is to increase tea tree mushroom commodity value, pluck when tea tree mushroom grows to when 7-8 divides maturation, 4 batches��5 batches mushrooms can be gone out according to above-mentioned management of producing mushroom.
2. the method for a kind of selenium enriched tea mushroom according to claim 1 cultivation, wherein, select the salt of resistance to selenium and the cultivated strain Jiangxi tea AS-1 that selenium bioaccumulation ability is strong, and it is carried out the salt of resistance to selenium domestication, when taming, domestication liquid sodium selenite aqueous solution is added in original seed and cultivar domestication substratum and carry out inoculating and cultivating, original seed and cultivar tame culture medium raw material and massfraction is: wood chip 35%, cotton seed hulls 33%, wheat bran 25%, Semen Maydis powder 5%, gypsum 1%, phosphate fertilizer 1%, add water to it and make its water content 60%��65%, the Sodium Selenite massfraction in wild Oryza species that adds water is 0.04%.
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