CN102668878A - Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium - Google Patents
Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium Download PDFInfo
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Abstract
The invention relates to the technical field of cultivation of flammulina velutipes, and particularly to a method for cultivating flammulina velutipes by using an aquilaria-sinensis-chip edible mushroom culture medium. The aquilaria-sinensis-chip edible mushroom culture medium used in the method is composed of the following raw materials in percentages by weight: 10-60% of aquilaria sinensis chips, 10-35% of rice bran, 0-60% of corncobs, 0-20% of cornmeal, 0-20% of cottonseed hulls, 0.5-1.5% of calcium carbonate, and 0.5-1% of quicklime. The method for cultivating flammulina velutipes by using the aquilaria-sinensis-chip edible mushroom culture medium comprises the following steps of: a, raw material treatment: accumulating the aquilaria sinensis chips outdoors for more than three months, wherein the aquilaria sinensis chips keeps wet by being regularly watered during the accumulation process; b, blending; c, bottling; d, sterilizing; e, inoculating; f, mycelium culture; g, mycelium stimulation; and h, fruiting culture management. The flammulina velutipes cultivated by the method disclosed by the invention is good in quality, high in productivity, high in single yield, high in biotransformation rate, rich in nutritive ingredients, very fragrant, sweet, fresh and tender, and has a healthcare function and a medicinal value.
Description
Technical field
The present invention relates to the golden mushroom plantation technical field, be specifically related to method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes.
Background technology
All can use wood chip in the conventional formulation that Asparagus is cultivated, but employed wood chip kind is more single, is main with coniferals wood chips such as pine tree wood, Chinese fir wood mainly.But coniferals wood chips such as pine tree wood, Chinese fir wood contain a large amount of greases and aromatic substance, and wherein, aromatic substance is a noxious material, and grease and aromatic substance can seriously hinder hypha of edible fungus growth and fruit body development.If without ungrease treatment, this type coniferous tree wood chip is the raw material that can not directly be used as culture medium of edible fungus.In the prior art, the coniferous tree wood chip is carried out ungrease treatment, generally need the method through boiling, distillation, its technology is numerous and diverse, and cost is high, and effect is also bad.
In recent years; Many local miscellaneous resources advantages of local broadleaf tree that combine are arranged; Explore actively and be used for the kind kind of culture medium of edible fungus,, developed multiple deciduous species such as mulberry tree wood, rubber trees, birchwood, willow wood successively according to domestic authoritative edible mushroom bibliographical information with wood chip.For the raw material as culture medium of edible fungus, hardwood sawdust is compared with the coniferous tree wood chip, has value preferably.
The fragrant tree of tabernaemontanus bulrush is unique deciduous species with the local name in Dongguan in the Chinese trees, has the plantation in more than 1000 year historical in the Dongguan, has the deep historical cultural deposits.At present, the Dongguan City, Guangdong Province is planted the fragrant tree of tabernaemontanus bulrush in a large number, development tabernaemontanus bulrush incense culture industry, and medical value that the research promotion tabernaemontanus bulrush is fragrant and health are worth, and have developed series of products such as tabernaemontanus bulrush scented tea, incense drug, sesame oil, spices, perfume.With tabernaemontanus bulrush perfume (or spice) is that the Chinese medicine of prescription surpasses hundred kinds, and tabernaemontanus bulrush is fragrant to boil waterly can treat scytitis such as pruitus, burns the fragrant smog that produces of tabernaemontanus bulrush and can purify air sterilizing.But, there is not the pertinent literature report temporarily, the fragrant tabernaemontanus bulrush incense wood bits of setting of tabernaemontanus bulrush of the growth in the Dongguan are opened up utilization.
Summary of the invention
The objective of the invention is to deficiency to prior art; Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes is provided; Utilize Asparagus that method provided by the present invention plants not only productive rate height, quality better, abundant nutrients; Fragrant and sweet especially fresh and tender, and have health care and medical value.Wherein, the tabernaemontanus bulrush incense wood bits that only are utilized in the fragrant tree of tabernaemontanus bulrush of Dongguan growth carry out cultivating flammulina velutipes, just can reach above-mentioned technical purpose.
To achieve these goals, the present invention adopts following technical scheme:
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and said tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 10%-60%
Rice bran 10%-35%
Corncob 0%-60%
Corn flour 0%-20%
Cotton seed hulls 0%-20%
Calcium carbonate 0.5%-1.5%
Quicklime 0.5%-1%;
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are open-air to be piled up 3 months ~ 12 months, in banking process, kept tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice;
C, bottling;
D, sterilization;
E, inoculation;
F, cultural hypha;
G, mycelium stimulation;
H, mushroom producing culture management.
Concrete, with the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, said raw material is placed agitator, do earlier and stirred 15 minutes ~ 30 minutes, add the water stirring 15 minutes ~ 30 minutes of wet then, obtain composts or fertilisers of cultivating;
C, bottling: said composts or fertilisers of cultivating is sent to bottling machine by pipeline, by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 121 ℃ ~ 123 ℃, and sterilization time is 30 minutes ~ 90 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing;
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Said mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1cm～2cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.
In the technique scheme, the moisture of the composts or fertilisers of cultivating that obtains in the step b spice is controlled between 62% ~ 64%.
In the technique scheme, in the step b spice, the time of said dried stirring is 20 minutes, and the time of said wet stirring is 20 minutes.
In the technique scheme, in the step f cultural hypha, the temperature of said culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated.
In the technique scheme, in the step h mushroom producing culture management, saidly urge the flower bud stage to be called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 13 ℃～16 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.
In the technique scheme, in the step h mushroom producing culture management, the said inhibition stage is: long during to 1cm～2cm when the mushroom bud, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃～6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 1 h ~ 2h that carries out 200LX every day.
In the technique scheme, in the step h mushroom producing culture management, the said encapsulate sheet stage is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
In the technique scheme, in the management of step h mushroom producing culture, said indoor temperature of all educating the stage should be controlled at 6 ℃～8 ℃, and air humidity remains on more than 75%; Light is main with dark, carries out the illumination of 300LX in per 3 days ~ 5 days, and each light application time is 30 minutes ~ 45 minutes.
In the technique scheme, in the step h mushroom producing culture management, said harvest stages is: when the fruit body of Asparagus is grown to above film 1cm～2cm, get into harvest stages.
The present invention compared with prior art, beneficial effect is:
1) in the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes provided by the invention; Because the fragrant tabernaemontanus bulrush incense wood bits of setting of tabernaemontanus bulrush with the growth in the Dongguan replace traditional wood chip, and tabernaemontanus bulrush incense wood bits can provide abundant cellulose, hemicellulose, lignin and special flavor component for the growth of Asparagus, thereby the Asparagus quality better that adopts method of the present invention to plant; Fragrant and sweet especially fresh and tender; Rounded and the homogeneous of mushroom cap, the diameter of mushroom cap are about 5mm, and " parachute-opening " phenomenon can not appear in the mushroom cap; The mushroom body is as white as polished jade, and mushroom handle consolidation is not loose.
2) adopt the productive rate of the Asparagus that method of the present invention plants to increase; Adopting effective radical of the Asparagus that traditional sawdust medium plants out is 200 ~ 400; Adopting effective radical of the Asparagus that method of the present invention plants is 400 ~ 600; Therefore, adopt the productive rate of the Asparagus that method of the present invention plants to increase nearly 1 times.
3) method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes provided by the invention, the single rate of the Asparagus that is planted is big, and the average single rate of the culture bottle of each 1200mL is 360g.
4) adopt the biological transformation ratio of the Asparagus that method of the present invention plants high, biological transformation ratio surpasses 110%; And adopt the biological transformation ratio of the Asparagus of traditional sawdust medium plantation to be generally 80%, therefore, the biological transformation ratio of the Asparagus that method of the present invention plants is 1.4 times of biological transformation ratio of the Asparagus of traditional sawdust medium plantation.
5) Asparagus that adopts method of the present invention to plant, fragrant and sweet tender and crisp, abundant nutrients especially is rich in lysine and arginine, is of value to children's brain cell development, has great health care function; And the Asparagus of being cultivated contains proflavin, and that proflavin has is anticancer, reducing blood lipid, dietotherapy function and medical value such as protect the liver.
6) method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes of the present invention is simple.
Embodiment
Below in conjunction with embodiment the present invention is further described.
embodiment 1.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 35kg, rice bran 20kg, corncob 30 kg, corn flour 13 kg, calcium carbonate 1.5 kg, quicklime 0.5 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 20 minutes, add the water stirring 20 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 62% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 121 ℃, and sterilization time is 50 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 15 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 13 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and illumination 1 h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 3 days, and each light application time is 30 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1cm, get into harvest stages.
Wherein, the Asparagus after gathering is in time packed after need cutting old root.Culture bottle after having gathered, culturing room is pulled out in unification, delivers to and digs the bottle district, through automatic scratching machine used medium in the culture bottle is dug totally, delivers to bottling after the culture bottle of sky is collected and distinguishes, and gets into next time and produces.In addition, be rich in plant growing nutrition, can carry out the mushroom slag and collect, and the mushroom slag is delivered to fertilizer plant be processed into the plant organic bio-fertilizer owing to use in the medium later.
Wherein, tabernaemontanus bulrush incense wood bits mainly play the effect of preserving moisture and ventilating in culture medium prescription, for the Asparagus growth provides cellulose, hemicellulose and lignin; Rice bran contains the Asparagus necessary whole nutrient that grows, for Asparagus provides crude protein and nitrogen free extract, and the required C/N ratio of adjustment Asparagus growth; Corncob mainly provides carbon source, raw fiber and nitrogen free extract, and has the effect of suction, water conservation and ventilation; Corn flour is that the growth of Asparagus provides crude protein, nitrogen free extract, raw fiber etc.; Calcium carbonate and quicklime are mainly used in the pH value of regulating medium, and calcium ion are provided for the growth of Asparagus.
Wherein, the pressure in the high steam moist heat sterilization mentioned of the present invention is 1Kg/cm
2~ 1.25Kg/cm
2
embodiment 2.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 50kg, rice bran 33.5 kg, cotton seed hulls 15 kg, calcium carbonate 1 kg, quicklime 0.5 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 15 minutes, add the water stirring 15 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 64% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 123 ℃, and sterilization time is 30 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 20 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 2cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 16 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 2cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 2h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 8 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 5 days, and each light application time is 45 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 2cm, get into harvest stages.
embodiment 3.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 25kg, rice bran 29 kg, corncob 45 kg, calcium carbonate 0.5 kg, quicklime 0.5 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 25 minutes, add the water stirring 30 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 63% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the steam moist heat sterilization, sterilising temp is 122 ℃, and sterilization time is 60 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 18 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1.5cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 15 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1.5cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 5 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 1.5h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 7 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 4 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1.5cm, get into harvest stages.
embodiment 4.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 35kg, rice bran 18.7 kg, corncob 30 kg, corn flour 15 kg, calcium carbonate 0.8kg, quicklime 0.5 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 30 minutes, add the water stirring 25 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 62.5% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 121.5 ℃, and sterilization time is 70 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 16 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1.8cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 14 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1.2cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 1.2h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 7 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 5 days, and each light application time is 35 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1.8cm, get into harvest stages.
embodiment 5.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 50kg, rice bran 33 kg, cotton seed hulls 15 kg, calcium carbonate 1kg, quicklime 1 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 20 minutes, add the water stirring 15 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 63.5% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 123 ℃, and sterilization time is 45 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 19 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1.6cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 13 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1.8cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 2h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 3 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1.2cm, get into harvest stages.
embodiment 6.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 25kg, rice bran 23 kg, corncob 50 kg, calcium carbonate 1kg, quicklime 1 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 15 minutes, add the water stirring 20 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 64% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 122.5 ℃, and sterilization time is 90 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 17 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1.4cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 13.5 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1.3cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 5 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 1.6h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 8 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 4 days, and each light application time is 45 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1.4cm, get into harvest stages.
embodiment 7.
Method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes; It is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw material: tabernaemontanus bulrush incense wood bits 60kg, rice bran 10 kg, corn flour 20 kg, cotton seed hulls 8 kg, calcium carbonate 1.4kg, quicklime 0.6 kg.
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, raw material is placed agitator, do earlier and stirred 20 minutes, add the water stirring 26 minutes of wet then, obtain composts or fertilisers of cultivating, wherein, the moisture of composts or fertilisers of cultivating is 63% of a composts or fertilisers of cultivating total amount;
C, bottling: composts or fertilisers of cultivating is sent to bottling machine by pipeline, and by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the composts or fertilisers of cultivating; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 121 ℃, and sterilization time is 55 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing; In the present embodiment, the culture bottle of each 1200ml inserts 20 gram bacterial classifications, evenly receives the composts or fertilisers of cultivating surface, and wherein, the outside with seed bottle before the inoculation disinfects in alcohol, and the old mycoderma of removing bacterial classification top re-uses.
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate, wherein, the temperature of culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1.7cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.Wherein, whole mushroom producing culture administrative time is 30 days.
Concrete, the flower bud stage of urging of mushroom producing culture management is called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 15 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.In the present embodiment, carry out humidification with the ultrasonic humidifier.
Concrete, the inhibition stage of mushroom producing culture management is: when mushroom bud length arrived 1.7cm, mushroom producing culture entered into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 2h that carries out 200LX every day.In the inhibition stage, though mushroom body poor growth is very neat, strong, strong.Wherein, the inhibition stage generally needs about 10 days.
Concrete, the encapsulate sheet stage of mushroom producing culture management is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
Concrete, the indoor temperature in the stage of all educating of mushroom producing culture management should be controlled at 6 ℃, and air humidity remains on more than 75%; Light is main with dark, per illumination of carrying out 300LX in 5 days, and each light application time is 40 minutes.
Concrete, the harvest stages of mushroom producing culture management is: when the fruit body of Asparagus is grown to above film 1.6cm, get into harvest stages.
Should be noted that at last; Above embodiment is only in order to explain technical scheme of the present invention; But not to the restriction of protection domain of the present invention, although with reference to preferred embodiment the present invention has been done explanation at length, those of ordinary skill in the art is to be understood that; Can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the essence and the scope of technical scheme of the present invention.
Claims (10)
1. with the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it is characterized in that: it is to cultivate Asparagus with tabernaemontanus bulrush incense wood bits culture medium of edible fungus, and said tabernaemontanus bulrush incense wood bits culture medium of edible fungus is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 10%-60%
Rice bran 10%-35%
Corncob 0%-60%
Corn flour 0%-20%
Cotton seed hulls 0%-20%
Calcium carbonate 0.5%-1.5%
Quicklime 0.5%-1%;
With the method for tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes, it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice;
C, bottling;
D, sterilization;
E, inoculation;
F, cultural hypha;
G, mycelium stimulation;
H, mushroom producing culture management.
2. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 1, it is characterized in that: it may further comprise the steps:
A, raw material are handled: tabernaemontanus bulrush incense wood bits are piled up more than 3 months in the open, in banking process, keep tabernaemontanus bulrush incense wood bits moist through watering regularly; That rice bran, corncob, corn flour, cotton seed hulls, calcium carbonate, quicklime require is fresh, do not lump, do not have and go mouldy and do not have worm;
B, spice: according to above-mentioned formula ratio raw materials weighing, said raw material is placed agitator, do earlier and stirred 15 minutes ~ 30 minutes, add the water stirring 15 minutes ~ 30 minutes of wet then, obtain composts or fertilisers of cultivating;
C, bottling: said composts or fertilisers of cultivating is sent to bottling machine by pipeline, by the bottling machine composts or fertilisers of cultivating of packing into automatically in the culture bottle; Utilize perforator then, the composts or fertilisers of cultivating in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the composts or fertilisers of cultivating center is a big hole, and four duck eyes evenly distribute around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then;
D, sterilization: after the bottling capping, at once culture bottle is carried out the high steam moist heat sterilization, sterilising temp is 121 ℃ ~ 123 ℃, and sterilization time is 30 minutes ~ 90 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃, just can send into transfer room and inoculate after finishing;
E, inoculation: under aseptic condition, carry out mechanical automatic vaccination through inoculation device with the bacterial classification of choosing;
F, cultural hypha: after inoculation finishes, send into culturing room at once and cultivate; Preceding 5 days of cultural hypha is that mycelia recovers the stage, and the mycelia respiratory capacity is less, and can ventilate 2～3 times every day to culturing room, each 15～30 minutes; Get into cultural hypha after the 6th day, the mycelia respiratory capacity begins to increase gradually, and at this moment ventilation equipment is set to often open, and promptly sends into fresh air continually, discharges indoor carbon dioxide; Inoculation back 23～25 days, mycelia cover with whole composts or fertilisers of cultivating surface;
G, mycelium stimulation: after the mycelia of culture bottle is covered with, cultivate and finish, should carry out mycelium stimulation immediately; Said mycelium stimulation is exactly old bacterial classification piece and a mycoderma of removing culture bottle the top 1cm～2cm through mushroom culturing device automatically, takes place simultaneously from media surface to impel fruit body;
H, mushroom producing culture management: be divided into and urge flower bud stage, inhibition stage, encapsulate sheet stage, all educate stage, harvest stages.
3. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 2, it is characterized in that: the moisture of the composts or fertilisers of cultivating that obtains in the step b spice is controlled between 62% ~ 64%.
4. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 3, it is characterized in that: in the step b spice, the time of said dried stirring is 20 minutes, and the time of said wet stirring is 20 minutes.
5. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 2, it is characterized in that: in the step f cultural hypha, the temperature of said culturing room is controlled at about 18 ℃, and air humidity is controlled at below 75%, and lucifuge is cultivated.
6. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 5 is characterized in that: in the management of step h mushroom producing culture, saidly urge the flower bud stage to be called the stage of sprouting again; The indoor temperature of urging the flower bud stage was 13 ℃～16 ℃ in order to urge the flower bud stage in preceding 7 days of the mushroom producing culture process, and indoor humidity remains on more than 95%, and indoor carbon dioxide concentration is controlled at below 0.1%, and suitably ventilate every day.
7. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 6 is characterized in that: in the management of step h mushroom producing culture, the said inhibition stage is: long during to 1cm～2cm when the mushroom bud, mushroom producing culture enters into the inhibition stage; The indoor temperature in this stage is controlled at 4 ℃～6 ℃, and humidity remains on more than 85%, and gas concentration lwevel is controlled at below 0.1%, and ventilate to every day, and the illumination 1 h ~ 2h that carries out 200LX every day.
8. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 7 is characterized in that: in the management of step h mushroom producing culture, the said encapsulate sheet stage is: when the mushroom body grows to when exceeding the bottleneck 2cm left and right sides, promptly enter into the encapsulate sheet stage; This stage needs to live bottleneck with the film circle, so that cultivation bottleneck top forms a small-sized gas concentration lwevel district, to impel the mushroom handle growth of Asparagus, suppresses the growth of mushroom cap, and Asparagus is grown by fan shape.
9. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 8, it is characterized in that: in the management of step h mushroom producing culture, said indoor temperature of all educating the stage should be controlled at 6 ℃～8 ℃, and air humidity remains on more than 75%; Light is main with dark, carries out the illumination of 300LX in per 3 days ~ 5 days, and each light application time is 30 minutes ~ 45 minutes.
10. the method with tabernaemontanus bulrush incense wood bits culture medium of edible fungus cultivating flammulina velutipes according to claim 9; It is characterized in that: in the step h mushroom producing culture management, said harvest stages is: when the fruit body of Asparagus is grown to above film 1cm～2cm, get into harvest stages.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210139688.2A CN102668878B (en) | 2012-05-08 | 2012-05-08 | Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium |
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| CN103172434A (en) * | 2012-10-29 | 2013-06-26 | 上海雪榕生物科技股份有限公司 | Process for improving whiteness of needle mushrooms |
| CN103508797A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Enokitake cultivation material using hargil as raw material and preparation method thereof |
| CN103980048A (en) * | 2014-05-22 | 2014-08-13 | 徐州绿维现代农业科技有限公司 | Preparation raw material and cultivation method of flammulina velutipes |
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| CN104119126A (en) * | 2013-04-26 | 2014-10-29 | 如意情集团股份有限公司 | Production process for flammulina velutipes medium |
| CN104151086A (en) * | 2014-09-01 | 2014-11-19 | 四川大自然惠川食品有限公司 | Culture medium and culture method of flammulina velutiper |
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| CN105165399A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for adjusting production states of edible fungi through measurement of respiratory capacity of edible fungi |
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| CN110583361A (en) * | 2019-10-22 | 2019-12-20 | 富川生物科技(江苏)有限公司 | Edible fungus cultivation method |
| CN113207553A (en) * | 2021-06-08 | 2021-08-06 | 上海光明森源生物科技有限公司 | Flammulina velutipes strain medium preservation method for improving strain stability and medium used by method |
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| CN104119126A (en) * | 2013-04-26 | 2014-10-29 | 如意情集团股份有限公司 | Production process for flammulina velutipes medium |
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| CN104956912A (en) * | 2015-06-09 | 2015-10-07 | 广西大学 | Cultivation method for golden mushroom |
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| CN108293592A (en) * | 2017-10-17 | 2018-07-20 | 辽宁天赢生物科技股份有限公司 | A method of cultivating needle mushroom using sorghum flour mixture |
| CN108029446A (en) * | 2017-11-28 | 2018-05-15 | 广东代米生物科技有限公司 | Agriculture and forestry organic waste material is used for the production method of the mushroom industrialized circulation of acupuncture needle |
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| CN108967033A (en) * | 2018-06-26 | 2018-12-11 | 福建神农菇业股份有限公司 | Bottle cultivated edible fungi cultural method |
| CN108834882A (en) * | 2018-08-21 | 2018-11-20 | 长沙县国进食用菌专业合作社 | A kind of faint yellow needle mushroom and its breeding method |
| CN110073900A (en) * | 2019-05-31 | 2019-08-02 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of culture medium for golden mushroom and its preparation method and application containing chicken feather degradation product |
| CN110073900B (en) * | 2019-05-31 | 2022-03-11 | 广东省农业科学院蚕业与农产品加工研究所 | Flammulina velutipes culture medium containing chicken feather degradation product and preparation method and application thereof |
| CN110583361A (en) * | 2019-10-22 | 2019-12-20 | 富川生物科技(江苏)有限公司 | Edible fungus cultivation method |
| CN113207553A (en) * | 2021-06-08 | 2021-08-06 | 上海光明森源生物科技有限公司 | Flammulina velutipes strain medium preservation method for improving strain stability and medium used by method |
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