CN102771314A - Method for culturing selenium-enriched cordyceps militaris - Google Patents
Method for culturing selenium-enriched cordyceps militaris Download PDFInfo
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Abstract
The invention provides a method for culturing selenium-enriched cordyceps militaris. The method comprises the steps of (1) domestication of tolerance of cordyceps sinensis strains of selenium, (2) preparation and inoculation of a selenium-enriched culture medium and (3) selenium-enriched culture of fruiting bodies. The method has the following beneficial effects: the selenium-enriched cordyceps sinensis culture effect can be improved, that is, the enrichment factor in the prior art is less than 1 and is improved to be above 4.85; the content of total selenium in the fruiting bodies obtained through culture can reach 4-100mg/kg; the organic selenium in the selenium forms is above 90%; and the enrichment factor and the absorption and transformation efficiency are greatly improved.
Description
Technical field
The present invention relates to a kind of method of cultivating rich selenium Cordyceps sinensis, belong to the functional health care product production technical field.
Background technology
Selenium is the trace element of needed by human, and is closely bound up with health, and that selenium has is anticancer, protection heart, treating liver necrosis, control myopia and cataract, detoxifcation, raising immunity, delay senility and strengthen multiple pharmacological effect such as reproductive function.According to incompletely statistics, have more than 40 countries and regions to lack selenium in the world, and China there is 72% area to belong to lack the selenium area, therefore, seek and develop rich selenium product, take in sufficient selenium to guarantee human body, just paid close attention to by the various countries scientific worker.
Northern Chinese caterpillar Fungus has another name called Cordyceps militaris, on biosystematics, belongs to Ascomycetes, the Cordyceps of fungi together with Chinese Cordyceps sinensis.Cordycepin is a kind of 3 '-deoxyadenosine of from the medium of Cordyceps militaris, separating in nineteen fifty; It has significant pharmacological action; Like bacteriostasis, antitumor action, inhibition virus function, and immunologic function, control cardiovascular and cerebrovascular disease etc. have been increased.Modern scientific research shows that cordycepin is the important active component of northern CORDYCEPS, and cordycepin content is tens of times of Cordyceps sinensis in the northern CORDYCEPS, is the main source that obtains cordycepin.At present the artificial cultivation of northern Chinese caterpillar Fungus is achieved success, a large amount of pharmacology and chemical analysis confirmation, and northern Chinese caterpillar Fungus and natural Cordyceps sinensis composition, pharmacology are basic identical, its functional component cordycepin even be higher than Cordyceps sinensis.Northern Chinese caterpillar Fungus is as the substitute of wild Chinese caterpillar fungus, and the market space is big.
Northern Chinese caterpillar Fungus can be attached to the metabolism of selenium through the Chinese caterpillar fungus hypha intracellular matter on the big molecular activity, is converted into organic selenium polysaccharide and selenoprotein, thus the intrinsic and collaborative physiological action of performance selenium and northern Chinese caterpillar Fungus.At present existing partial monopoly is carried out the cultivation and the production of artificial rich selenium north CORDYCEPS; " selenium-rich cordyceps culturing method (number of patent application 201010252973.6) ", " a kind of breeding method of Se-enriched cordyceps militaris (number of patent application 200710010750.7) ", " the artificial culture method of selenium-enriched cordceps militaris and the application of fruit body (number of patent application 200610046673.6) ", " method for artificially culturing selenium-rich Northern Chinese caterpillar fungus " technical schemes such as (number of patent applications 93117344.2) all are that the rich selenium that carries out northern Chinese caterpillar Fungus is strengthened; But what technique scheme adopted all is in medium, to add inorganic selenite or spray the selenium fertilizer nutrition liquid that contains selenite; The employing selenite is that the source Chinese caterpillar fungus of selenium element is difficult to absorb; Availability is not high; So Chinese caterpillar fungus of adopting said method to cultivate; The concentration coefficient of selenium is not high, and the fruit body Se content of the northern Chinese caterpillar Fungus of cultivating in these technical schemes is up to 3.83～81.95 μ g/g, and its corresponding cultivate material selenium concentration is 1～32 mg/L; And the shared large percentage of inorganic selenium in its selenium form of the cordyceps militaris sporocarp that obtains is difficult to improve nutrition and the medical value of Chinese caterpillar fungus." a kind of breeding method of Se-enriched cordyceps militaris " (number of patent application 201010621575.7), " the female breeding method of planting of Se-enriched cordyceps militaris " technical schemes such as (number of patent applications 201110279521.1) are then improved selenium source; Adopt the selenium source of organic selenium yeast as cultivate material; But the organic selenium yeast is because cell wall is too thick; Chinese caterpillar fungus can't effectively absorb selenium, causes se use efficiency lower, and the concentration coefficient of selenium is very low; Adopt selenium yeast to make that the medium selenium concentration is 50 mg/L in the technique scheme, the Se-enriched cordyceps militaris Se content of cultivation is merely 35～50 mg/L.And organic selenium yeast cost is higher, if broken wall treatment is also comparatively complicated, and cost is also higher, is not suitable for cultivating selenium-rich cordyceps as selenium source.
Summary of the invention
The objective of the invention is to provide a kind of cultural method of Se-enriched cordyceps militaris; Can increase substantially the Se content of the fruit body of northern Chinese caterpillar Fungus with respect to the northern Chinese caterpillar Fungus of prior art cultivation with this cultural method; And; The selenium form overwhelming majority is an organic selenium in the cordyceps militaris fruit body, selenium form safety.
The purpose of foregoing invention realizes through following technical scheme, comprises following process step:
(1) carry out actication of culture at the PDA solid culture medium, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months;
(2) selenium of Coragyceps militaris bacterium domestication: utilizing a series of selenium concentration that contains is that the liquid nutrient medium of 1～50 mg/kg carries out the selenium domestication to the Cordyceps militaris bacterial classification; Obtain tolerating the Coragyceps militaris bacterium under the concentration at last, then selenium concentration be in the liquid nutrient medium of 20 mg/kg enlarged culture as the cultivate material original seed.Wherein the prescription of liquid nutrient medium is: powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml.Adding the selenium material that contains of 2.19～109.5 mg according to the selenium concentration of the liquid nutrient medium of design then, to form selenium concentration be the liquid nutrient medium of 1～50 mg/kg.The above-mentioned selenium material that contains can be in selenite, selenate, high selenium plant or the microorganism one or more;
(3) preparation of the cultivate material additive of Se-enriched cordyceps militaris: adopt inorganic selenium to contain the selenium material as raw material through the vegetalitas of secondary biological transformation; Through pulverizing, screening, dry, design the required cultivate material additive of cultivation of selenium-rich northern Chinese caterpillar Fungus on this basis as the selenium source in the cultivation process.Above-mentioned selenium-enriched plant material can be one or more mixing natural or in the high selenium wheat flour of artificial-strengthening, high selenium corn flour, high selenium analysis for soybean powder, high selenium broccoli powder, high selenium Chinese milk vetch powder or high selenium clover pollen;
(4) the rich selenium of fruit body is cultivated: the prescription of a, the rich seleno culture medium of fruit body is following: it is made up of rice, selenate, glutelin hydrolyzate; Glutelin hydrolyzate system is to be rich in the wheat class of methionine, cystine and cysteine or the enzymolysis aqueous solution of rice glutelin; Mark compares by weight: 20～25 parts of rice weight fractions, and 30～35 parts of glutelin hydrolyzates, 1～10 part in selenium-enriched plant material powder, the medium selenium concentration of its formation is 1～20 mg/kg; B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of the fruit body of above-mentioned prescription, insert above-mentioned original seed through the selenium domestication; Incubation time 40～50 days; Bacteria developing period management wherein: the cultural hypha temperature is controlled at 10～25 ℃, air humidity 60%～70%, and dark condition is cultivated fully; Colour-change period management: cultivation temperature is at 18～26 ℃, every day light application time more than 10 hours, carrying out continuous alternating temperature simultaneously stimulates, day and night temperature is between 10～20; The sporophore growth stage management: after button occurring on the charge level, temperature suitably reduces, and is controlled at 19～23 ℃, air humidity 80%～90%, and light stimulation promote fruit body to form, and it is strong, bright in colour to grow; Gather: when the fruit body height reaches 5-8 ㎝, the oven dry of can gathering of about 50% fruit body ultimate swelling.
Wherein, above-mentioned selenium-enriched plant material can be one or more mixing natural or in the high selenium wheat flour of artificial-strengthening, high selenium corn flour, high selenium analysis for soybean powder, high selenium broccoli powder, high selenium Chinese milk vetch powder or high selenium clover pollen.
Above-mentioned Se-enriched cordyceps militaris cultural method; One of its method is that PDA solid culture based formulas is following: it is made up of potato, glucose, KH2PO4, agar, water; By ratio of weight and the number of copies: 150～250 parts in potato, 15～25 parts of glucose, 1.7～2.3 parts of KH2PO4; 13～20 parts in agar, 950～980 parts in water.
Beneficial effect of the present invention shows:
At first plant and just carry out the selenium domestication in the cultivation the mother of northern Chinese caterpillar Fungus; Make it can adapt to the selenium environment of high concentration; Carry out the accumulation and the absorption of selenium, its efficient that absorbs selenium of the rich selenium bacterial classification that obtains under this environment is high, is more conducive to Chinese caterpillar fungus and from medium, accumulates selenium;
Secondly, adopt natural or through the vegetable material of artificial-strengthening as selenium source, the selenium overwhelming majority wherein all is an organic selenium; Its toxic action to northern Chinese caterpillar Fungus is littler, and is more conducive to absorbing of Chinese caterpillar fungus, with respect to other inorganic seleniums or yeast selenium; Can reach more accumulative effect; Be generally less than 1 with respect to concentration coefficient in the selenium-enriching cultivation of the prior art, the concentration coefficient of selenium can reach 4.85 times among the present invention, and fruit body selenium concentration and cultivate material selenium concentration are linear between the two; Linear equation is y=4.86x (R2=0.98); The total Se content of fruit body that cultivation obtains can reach 4～100 mg/kg, and the organic selenium ratio all is greatly improved with absorbing on the transformation efficiency at the enrichment multiple up to more than 90% in the selenium form.
Embodiment
Following examples are used to explain the present invention, but can not be used for limiting scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and unaccounted implementation condition is generally the condition in the normal experiment.
Embodiment 1: selenium concentration is the selenium domestication of northern Chinese caterpillar Fungus under 1～10 mg/kg
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) selenium of Coragyceps militaris bacterium domestication: utilize that to contain selenium concentration be that the liquid nutrient medium of 1～10 mg/kg carries out the selenium domestication to the Cordyceps militaris bacterial classification; Obtain tolerating the Coragyceps militaris bacterium under the concentration at last, wherein the prescription of liquid nutrient medium is: powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml.It is the liquid nutrient medium of 1～10 mg/kg that the sodium selenite that adds 2.19～21.9 mg according to the selenium concentration of the liquid nutrient medium of design then forms selenium concentration.
Embodiment 2: selenium concentration is the selenium domestication of northern Chinese caterpillar Fungus under 1～30 mg/kg
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) selenium of Coragyceps militaris bacterium domestication: utilize that to contain selenium concentration be that the liquid nutrient medium of 1～30 mg/kg carries out the selenium domestication to the Cordyceps militaris bacterial classification; Obtain tolerating the Coragyceps militaris bacterium under the concentration at last, wherein the prescription of liquid nutrient medium is: powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml.It is the liquid nutrient medium of 1～30 mg/kg that the sodium selenate that adds 2.19～65.7 mg according to the selenium concentration of the liquid nutrient medium of design then forms selenium concentration.
Embodiment 3: selenium concentration is the selenium domestication of northern Chinese caterpillar Fungus under 1～50 mg/kg
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) selenium of Coragyceps militaris bacterium domestication: utilize that to contain selenium concentration be that the liquid nutrient medium of 1～50 mg/kg carries out the selenium domestication to the Cordyceps militaris bacterial classification; Obtain tolerating the Coragyceps militaris bacterium under the concentration at last, wherein the prescription of liquid nutrient medium is: powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml.It is the liquid nutrient medium of 1～50 mg/kg that the yeast selenium that adds 2.19～109.5 mg according to the selenium concentration of the liquid nutrient medium of design then forms selenium concentration.
Embodiment 4: the rich selenium of cordyceps militaris fruit body is cultivated
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) the rich selenium of fruit body is cultivated:
The prescription of a, the rich seleno culture medium of fruit body is following: it is by rice, the additive by the configuration of selenium-rich corn powder, the enzyme hydrolyzate of rice glutelin; Mark compares by weight: 35 parts of solution weight umbers, 1 part in selenium-rich corn powder that 25 parts of rice parts by weight, glutelin hydrolyzate form, and finally forming selenium concentration is the rich seleno culture medium of 1 mg/kg;
B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of above-mentioned fruit body, insert the selenium-rich cordyceps bacterial classification that obtains through the selenium domestication, the bacterial classification weight of access is 5 % of the rich seleno culture medium of fruit body; Incubation time 45 days.Through hair tube reason, annesl management and sporophore growth stage management, the final fruit body that obtains northern Chinese caterpillar Fungus of cultivating, gathering gets final product;
(3) implementation result:
Through detecting, the biomass of northern Chinese caterpillar Fungus is compared with common control group, slightly improves; But not obvious, the total Se content of fruit body is 4.79 mg/kg (DW), is 10 times of common cultivation group; The selenium form is main with various seleno-amino acids mainly, and the organic selenium proportion reaches more than 90 %, and fruit body is in growth cycle; The relative standard deviation of selenium content can be controlled in 15%, can realize even selenium-enriching cultivation.
Embodiment 5: the rich selenium of cordyceps militaris fruit body is cultivated
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) the rich selenium of fruit body is cultivated:
The prescription of a, the rich seleno culture medium of fruit body is following: it is by rice, the additive by the configuration of Se-rich wheat powder, the enzyme hydrolyzate of rice glutelin; Mark compares by weight: 30 parts of solution weight umbers, 5 parts in Se-rich wheat powder that 25 parts of rice parts by weight, glutelin hydrolyzate form, and finally forming selenium concentration is the rich seleno culture medium of 10 mg/kg;
B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of above-mentioned fruit body, insert the selenium-rich cordyceps bacterial classification that obtains through the selenium domestication, the bacterial classification weight of access is 5 % of the rich seleno culture medium of fruit body; Incubation time 45 days.Through hair tube reason, annesl management and sporophore growth stage management, the final fruit body that obtains northern Chinese caterpillar Fungus of cultivating, gathering gets final product;
(3) implementation result:
Through detecting, the biomass of northern Chinese caterpillar Fungus is compared with common control group, slightly improves; But not obvious, the total Se content of fruit body is 48.2 mg/kg (DW), and the selenium form is main with various seleno-amino acids mainly; The organic selenium proportion reaches more than 90 %; Fruit body is in growth cycle, and the relative standard deviation of selenium content can be controlled in 15%, can realize even selenium-enriching cultivation.
Embodiment 6: the rich selenium of cordyceps militaris fruit body is cultivated
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) the rich selenium of fruit body is cultivated:
The prescription of a, the rich seleno culture medium of fruit body is following: it is by rice, the additive by rich selenium analysis for soybean powder configuration, the enzyme hydrolyzate of rice glutelin; Mark compares by weight: 30 parts of solution weight umbers, 10 parts of rich selenium analysis for soybean powder that 20 parts of rice parts by weight, glutelin hydrolyzate form, and finally forming selenium concentration is the rich seleno culture medium of 20 mg/kg;
B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of above-mentioned fruit body, insert the selenium-rich cordyceps bacterial classification that obtains through the selenium domestication, the bacterial classification weight of access is 5 % of the rich seleno culture medium of fruit body; Incubation time 45 days.Through hair tube reason, annesl management and sporophore growth stage management, the final fruit body that obtains northern Chinese caterpillar Fungus of cultivating, gathering gets final product;
(3) implementation result:
Through detecting, the biomass of northern Chinese caterpillar Fungus is compared with common control group, slightly improves; But not obvious, the total Se content of fruit body is 97.02 mg/kg (DW), and the selenium form is main with various seleno-amino acids mainly; The organic selenium proportion reaches more than 90 %; Fruit body is in growth cycle, and the relative standard deviation of selenium content can be controlled in 15%, can realize even selenium-enriching cultivation.
Embodiment 7: the rich selenium of cordyceps militaris fruit body is cultivated
(1) on the PDA medium, carry out the activation of bacterial classification, the excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months; The PDA culture medium prescription is the mark ratio by weight: get potato 200 g of filtrating, glucose 20 g, KH2PO4 2 g, agar 15 g, water 970 ml through the peeling liquor;
(2) the rich selenium of fruit body is cultivated:
The prescription of a, the rich seleno culture medium of fruit body is following: it is by rice, the additive by rich selenium broccoli powder configuration, the enzyme hydrolyzate of rice glutelin; Mark compares by weight: 30 parts of solution weight umbers, 10 parts of rich selenium broccoli powders that 20 parts of rice parts by weight, glutelin hydrolyzate form, and finally forming selenium concentration is the rich seleno culture medium of 30 mg/kg;
B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of above-mentioned fruit body, insert the selenium-rich cordyceps bacterial classification that obtains through the selenium domestication, the bacterial classification weight of access is 5 % of the rich seleno culture medium of fruit body; Incubation time 45 days.Through hair tube reason, annesl management and sporophore growth stage management, the final fruit body that obtains northern Chinese caterpillar Fungus of cultivating, gathering gets final product;
(3) implementation result:
Through detecting, the biomass of northern Chinese caterpillar Fungus is compared with common control group, slightly improves; But not obvious, the total Se content of fruit body is 146.58 mg/kg (DW), and the selenium form is main with various seleno-amino acids mainly; The organic selenium proportion reaches more than 90 %; Fruit body is in growth cycle, and the relative standard deviation of selenium content can be controlled in 15%, can realize even selenium-enriching cultivation.
Claims (10)
1. method of cultivating Se-enriched cordyceps militaris is characterized in that: may further comprise the steps: (1) aweto strain is to the tolerance domestication of selenium; (2) preparation of rich seleno culture medium and inoculation; (3) the rich selenium of fruit body is cultivated.
2. the method for cultivation Se-enriched cordyceps militaris according to claim 1 is characterized in that: the medium of the said tolerance domestication of carrying out selenium except basal medium, also comprise concentration be 1～50 mg/kg contain the selenium material.
3. the method for cultivation Se-enriched cordyceps militaris according to claim 2 is characterized in that: the said selenium material that contains is in selenite, selenate, high selenium plant or the microorganism one or more.
4. the method for cultivation Se-enriched cordyceps militaris according to claim 1; It is characterized in that: the said medium that carries out the tolerance domestication of selenium is a liquid nutrient medium; Its prescription is powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml, and the adding selenium concentration is the sodium selenite of 1～50 mg/kg.
5. the method for cultivation Se-enriched cordyceps militaris according to claim 1 is characterized in that: the selenium source material in the said rich seleno culture medium is the high selenium vegetable material through the secondary biological transformation.
6. the method for cultivation Se-enriched cordyceps militaris according to claim 5 is characterized in that: said selenium-enriched plant material can be one or more mixing natural or in the high selenium wheat flour of artificial-strengthening, high selenium corn flour, high selenium analysis for soybean powder, high selenium broccoli powder, high selenium Chinese milk vetch powder or high selenium clover pollen.
7. the method for cultivation Se-enriched cordyceps militaris according to claim 1 is characterized in that: the prescription of said rich seleno culture medium is: rice, selenate, glutelin hydrolyzate; Wherein glutelin hydrolyzate system is to be rich in the wheat class of methionine, cystine and cysteine or the enzymolysis aqueous solution of rice glutelin; Mark compares by weight: 20～25 parts of rice weight fractions, and 30～35 parts of glutelin hydrolyzates, 1～10 part in selenium-enriched plant material powder, the medium selenium concentration of its formation is 1～20 mg/kg.
8. the method for cultivation Se-enriched cordyceps militaris according to claim 1; It is characterized in that: saidly be: in rich seleno culture medium, insert original seed through the selenium domestication to the rich selenium cultured method of fruit body; Incubation time 40～50 days; Bacteria developing period management wherein: the cultural hypha temperature is controlled at 10～25 ℃, air humidity 60%～70%, and dark condition is cultivated fully; Colour-change period management: cultivation temperature is at 18～26 ℃, every day light application time more than 10 hours, carrying out continuous alternating temperature simultaneously stimulates, day and night temperature is between 10～20; The sporophore growth stage management: after button occurring on the charge level, temperature suitably reduces, and is controlled at 19～23 ℃, air humidity 80%～90%, and light stimulation promote fruit body to form, and it is strong, bright in colour to grow; Gather: when the fruit body height reaches 5-8 ㎝, the oven dry of can gathering of about 50% fruit body ultimate swelling.
9. the method for cultivation Se-enriched cordyceps militaris according to claim 1; It is characterized in that: carry out carrying out actication of culture at the PDA solid culture medium before the tolerance domestication of aweto strain to selenium; The excellent species of preserving in the refrigerator, activation was once on the PDA solid culture medium in per two months.
10. the method for cultivation Se-enriched cordyceps militaris according to claim 1 is characterized in that: comprise following steps: actication of culture is carried out at the PDA solid culture medium in (1); (2) selenium of Coragyceps militaris bacterium domestication: utilizing a series of selenium concentration that contains is that the liquid nutrient medium of 1～50 mg/kg carries out the selenium domestication to the Cordyceps militaris bacterial classification; Obtain tolerating the Coragyceps militaris bacterium under the concentration at last, then selenium concentration be in the liquid nutrient medium of 20 mg/kg enlarged culture as the cultivate material original seed; Wherein the prescription of liquid nutrient medium is: powdery glucose 20 g, analysis for soybean powder 10 g, vitamin B1 50 mg, magnesium sulfate 2 g, potassium dihydrogen phosphate 3 g, edible monosodium glutamate 1g, salt 2 g, water 1000 ml; It is the liquid nutrient medium of 1～50 mg/kg that the sodium selenite that adds 2.19～109.5 mg according to the selenium concentration of the liquid nutrient medium of design then forms selenium concentration; (3) preparation of the cultivate material additive of Se-enriched cordyceps militaris: adopt the selenium-rich corn powder as raw material,, design the required cultivate material additive of cultivation of selenium-rich northern Chinese caterpillar Fungus on this basis through pulverizing, screening, dry as the selenium source in the cultivation process; (4) the rich selenium of fruit body is cultivated: the prescription of a, the rich seleno culture medium of fruit body is following: it is made up of rice, selenate, glutelin hydrolyzate; Glutelin hydrolyzate system is to be rich in the wheat class of methionine, cystine and cysteine or the enzymolysis aqueous solution of rice glutelin; Mark compares by weight: 20～25 parts of rice weight fractions, and 30～35 parts of glutelin hydrolyzates, 1～10 part in selenium-rich corn powder, the medium selenium concentration of its formation is 1～20 mg/kg; B, the rich selenium cultural method of fruit body: in the rich seleno culture medium of the fruit body of above-mentioned prescription, insert above-mentioned original seed through the selenium domestication; Incubation time 40～50 days; Bacteria developing period management wherein: the cultural hypha temperature is controlled at 10～25 ℃, air humidity 60%～70%, and dark condition is cultivated fully; Colour-change period management: cultivation temperature is at 18～26 ℃, every day light application time more than 10 hours, carrying out continuous alternating temperature simultaneously stimulates, day and night temperature is between 10～20; The sporophore growth stage management: after button occurring on the charge level, temperature suitably reduces, and is controlled at 19～23 ℃, air humidity 80%～90%, and light stimulation promote fruit body to form, and it is strong, bright in colour to grow; Gather: when the fruit body height reaches 5-8 ㎝, the oven dry of can gathering of about 50% fruit body ultimate swelling.
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| CN105875194A (en) * | 2015-01-26 | 2016-08-24 | 孙备宽 | Planting method of selenium-enriched cordyceps militaris |
| CN107027516A (en) * | 2017-04-17 | 2017-08-11 | 滁州学院 | A kind of selenium-rich Periostracum cicadae, its cultural method and application |
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| CN103070011A (en) * | 2013-01-07 | 2013-05-01 | 浙江工业大学 | Preparation method of selenium-rich cordyceps sinensis mycelia |
| CN103030452A (en) * | 2013-01-17 | 2013-04-10 | 李卫旗 | Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris |
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| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
| CN103535187A (en) * | 2013-09-27 | 2014-01-29 | 苏州硒泰克生物科技有限公司 | Method for preparing ganoderma lucidum high in selenium content for food additive |
| CN104303825A (en) * | 2014-09-27 | 2015-01-28 | 江西省农业科学院农业应用微生物研究所 | Method for cultivating selenium-enriched agrocybe cylindracea |
| CN105875194A (en) * | 2015-01-26 | 2016-08-24 | 孙备宽 | Planting method of selenium-enriched cordyceps militaris |
| CN104871827A (en) * | 2015-06-11 | 2015-09-02 | 孙建然 | Artificially cultivated cordyceps sinensis and cultivation method thereof |
| CN107027516A (en) * | 2017-04-17 | 2017-08-11 | 滁州学院 | A kind of selenium-rich Periostracum cicadae, its cultural method and application |
| CN108476861A (en) * | 2018-03-04 | 2018-09-04 | 江西农业大学 | A kind of nunchakus spore Chinese caterpillar fungus strain and its cultural method |
| CN110463506A (en) * | 2018-05-12 | 2019-11-19 | 安徽过湾农业科技有限公司 | A kind of breeding method of selenium-rich cordyceps sinensis |
| CN109618815A (en) * | 2019-01-18 | 2019-04-16 | 福州隆利信生物制品有限公司 | The method for extracting defective material culture Chinese caterpillar fungus hypha using red yeast rice |
| CN109618815B (en) * | 2019-01-18 | 2021-04-16 | 福州隆利信生物制品有限公司 | Method for culturing cordyceps sinensis mycelia by using red yeast rice extraction residues |
| CN115500204A (en) * | 2022-05-09 | 2022-12-23 | 江苏中农科食品工程股份有限公司 | Method for cultivating edible fungi rich in organic selenium |
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