CN103305434A - Microecological preparation with dual functions of probiotics and organic selenium and preparation method of microecological preparation - Google Patents
Microecological preparation with dual functions of probiotics and organic selenium and preparation method of microecological preparation Download PDFInfo
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Abstract
The invention relates to a microecological preparation with dual functions of probiotics and organic selenium and a preparation method of the microecological preparation, in particular relates to a microecological preparation, namely a probiotic living selenium microecological preparation, with the dual functions of probiotics and organic selenium and a preparation method of the microecological preparation and belongs to the field of fermentation industry. The microecological preparation comprises saccharomyces cerevisiae, rhodopseudomonas palustris and lactobacillus plantarum, wherein the saccharomyces cerevisiae (se-enriched yeast) accounts for 30-50 percent of the total number of living bacteria; the rhodopseudomonas palustris accounts for 10-20 percent of the total number of living bacteria; the lactobacillus plantarum accounts for 30-50 percent of the total number of living bacteria; the content of biological selenium (based on Se) is 300-4800ppm; and the total number of living bacteria is more than or equal to 400 million/ml. The probiotic living selenium microecological preparation with the dual functions of probiotics and organic selenium can be widely applied to livestock breeding, aquaculture industry, planting industry, forestry and fruit growing, production of edible fungi and cultivation of Chinese herbal medicines, and can be used for producing organic selenium functional foods.
Description
Technical field
The present invention relates to a kind of probiotics with probiotic bacterium and organoselenium dual function and preparation method thereof, referring to especially a kind of probiotics with probiotic bacterium and organoselenium dual function----probiotic bacterium live body selenium probiotics and preparation method thereof the invention belongs to the fermentation industry field.
Background technology
Selenium is one of essential micronutrient element of human body and animal; it is the activeconstituents of animal body glutathion inside peroxidase; this kind of enzyme can be removed superoxide and the hydroxy radical qiao that produces in the zooblast respiratory metabolism; the biometric safeguard membrane integrity; thereby have the raising immunity of organisms, protection liver and anti-aging effects are arranged.Said as the authoritative Field doctor of world trace element: " selenium is as a nuclear bomb, measure very little very little, act on and power but all very large, in case be familiar with by people, will produce far-reaching influence to Jiankang ".
Along with the development of biomedical technology, trace element-selenium has caused people's great attention as a kind of nutritive element of needed by human.When selenium was mended in research, gap was very little between nutritional requirement and the toxic dose, is easy to cause poisoning.People often are divided into organoselenium and inorganic selenium two large classes to selenium compound, experiment showed, the selenium that biological method obtains, and can effectively assimilate in vivo, and toxicity are little, be beneficial to again in vivo to absorb, and be a kind of more satisfactory micro-element nutrition reinforcer.
Biological selenium-enriched is the effective way of obtaining organoselenium, and at present, biological selenium-enriched method mainly is microbe transformation method.The microorganism that is used for rich selenium has edible fungi and probiotic bacterium class etc., and wherein yeast, milk-acid bacteria, photosynthetic bacteria are the ideal rich selenium carriers of generally acknowledging at present, and the biopotency of the live body selenium in the probiotics bacterial is 10～20 times of inorganic selenium.
The research of probiotic bacterium and prebiotics not only enters at present climax both at home and abroad, and has formed powerful industry.Probiotic bacterium is present in tellurian each corner, atmosphere, water, edatope the inside, and useful bacterium or fungi mainly contains in the animal body: clostridium butyricum, milk-acid bacteria, bifidus bacillus, actinomycetes, yeast, photosynthetic bacteria etc.Probiotics refers to improve host's microecological balance and the microorganism live body of bringing into play beneficial effect, reaches the active bacteria formulation and the meta-bolites thereof that improve host health level and state of health.The most powerful product of function of research mainly is the composite reactive probiotics micro-ecological formulation of above all kinds of the composition of the microorganisms in the world at present.Probiotics is different from other medicines, and it can play the vital role of " sickly auxiliaryly control, not sick diseases prevention, anosis health care ".The emphasis of probiotics is " anosis health care ".
Functional probiotics is used for tightening and improving the various physiological functions of host's body, plays and prevents and cures diseases, and promotes to grow, and promotes physique, delays senility and expects long-lived purpose.Probiotic bacterium has the intestinal microflora of keeping balance, control gastrointestinal tract disease, produces multiple enzyme, synthetic multiple nutrients material, promotes growth, and the biological functions such as enhancing body immunity function are widely used in organic foodstuff production at present.
The Multifunction probiotics is adjusted physiological equilibrium by fostering normal microbial population, the performance Ant agonism, thus can get rid of pathogenic bacterium and conditioned pathogen invasion and attack, eliminate the residual of heavy metal.Extensively improve at present the functional material of aspect as the little ecology of the contaminate environment such as soil improvement, sewage purification.
The present invention is by adopting the simultaneously compound cultivation in containing the liquid nutrient medium of sodium selenite of functional bacterial strain of saccharomycetic rich selenium bacterial strain and photosynthetic bacteria, milk-acid bacteria, optimum culture condition, promote that inorganic selenium is converted into organoselenium, develop a kind of probiotics with probiotic bacterium and organoselenium dual function----probiotic bacterium live body selenium preparation.
Summary of the invention
One of purpose of the present invention is to provide a kind of probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function.
Above-mentioned purpose of the present invention reaches by the following technical programs:
A kind of probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function is characterized in that: comprise S. cervisiae, Rhodopseudomonas palustris, plant lactobacillus.
A kind of optimal technical scheme, it is characterized in that: described S. cervisiae (yeast rich in selenium) accounts for 30～50% of total viable count, described Rhodopseudomonas palustris accounts for 10～20% of total viable count, and described plant lactobacillus accounts for 30～50% (preferred 30～40%) of total viable count.
A kind of optimal technical scheme is characterized in that: described probiotic bacterium live body selenium probiotics, and wherein, biological selenium (take Se) content is 300～4800ppm; Total viable count 〉=400,000,000/ml.
A kind of optimal technical scheme is characterized in that: described probiotic bacterium live body selenium probiotics is liquid, and wherein, biological selenium (take Se) content is 300～1200ppm; Total viable count is 〉=4 hundred million/ml, is preferably 4～800,000,000/ml.
A kind of optimal technical scheme is characterized in that: described probiotic bacterium live body selenium probiotics is solid, wherein, biological selenium (in Se) content 1200～4800ppm, total viable count is 〉=15 hundred million/g, is preferably 15～3,000,000,000/g.
Another object of the present invention provides above-mentioned preparation method with probiotic bacterium live body selenium probiotics of probiotic bacterium and organoselenium dual function.
Above-mentioned purpose of the present invention reaches by the following technical programs:
A kind of preparation method with probiotic bacterium live body selenium probiotics of probiotic bacterium and organoselenium dual function, its step is as follows:
(1) preparation of yeast saccharomyces cerevisiae bacteria culture fluid gets three grades of seed culture fluids of S. cervisiae;
(2) preparation of Rhodopseudomonas palustris nutrient solution gets the Rhodopseudomonas palustris seed culture fluid;
(3) preparation of plant lactobacillus nutrient solution gets three grades of seed culture fluids of plant lactobacillus;
(4) with the seed culture fluid of S. cervisiae, Rhodopseudomonas palustris, three bacterial classification single culture of plant lactobacillus, carry out compound cultivation in the fermentor tank by the liquid complex medium that 10～30% inoculum size access total reducing sugar amount is 8～15%, sodium selenite concentration is 0.3～1.2g/L respectively, under 28～32 ℃ condition, intermittent stirring was cultivated 24～48 hours, static fermentation is 48～96 hours again, cultivates to make liquid probiotic bacterium live body selenium probiotics.
A kind of optimal technical scheme, it is characterized in that: described step (4) gained liquid probiotic bacterium live body selenium probiotics, add and account for the modified porous starch of gross weight 18～25% and 2～5% clinoptilolite powder, abundant stirring and evenly mixing, adopt high speed centrifugation spraying flash dryer under 45～65 ℃ of conditions, the expansion drying of spraying gets solid probiotic bacterium live body selenium probiotics.
A kind of optimal technical scheme, it is characterized in that: described S. cervisiae (yeast rich in selenium) accounts for 30～50% of total viable count, described Rhodopseudomonas palustris accounts for 10～20% of total viable count, and described plant lactobacillus accounts for 30～50% (preferred 30～40%) of total viable count.
In the probiotic bacterium live body selenium probiotics that makes, described S. cervisiae (yeast rich in selenium) accounts for 30～50% of total viable count, and described Rhodopseudomonas palustris accounts for 10～20% of total viable count, and described plant lactobacillus accounts for 30～40% of total viable count.
In the probiotic bacterium live body selenium probiotics that makes, biological selenium (take Se) content is 300～4800ppm; Total viable count 〉=400,000,000/ml.In the described liquid probiotic bacterium live body selenium probiotics, biological selenium (take Se) content is 300～1200ppm; Total viable count is 〉=4 hundred million/ml, is preferably 4～800,000,000/ml.In the described solid probiotic bacterium live body selenium probiotics, biological selenium (in Se) content 1200～4800ppm, total viable count is 〉=15 hundred million/g, is preferably 15～3,000,000,000/g.
Beneficial effect:
The present invention on the basis of great many of experiments, 1) select and turn selenium and the strongest S. cervisiae of selenium rich ability is main selenium-enriched probiotics bacterial classification; 2) select have coordinate colony balance, nutrition high, produce enzyme high, suppress the harmful bacteria growth, the Rhodopseudomonas palustris bacterial classification that improves the coenocorrelation function is the major function bacterial classification; 3) select that to have the plant lactobacillus that can stablize pH value, degraded heavy metal, biological preservative function be the major function bacterial classification; And its independent batch culture become yeast rich in selenium, probiotic bacterium original bacteria liquid, preservation is for subsequent use.
And then by a certain percentage, S. cervisiae stoste, Rhodopseudomonas palustris stoste, plant lactobacillus stoste, coupling is compounded in compound cultivation in the liquid nutrient medium that contains sodium selenite, and the control culture condition is by first two sections fermentation methods cultivations of aerobic rear anaerobism, cultivation makes probiotic bacterium live body selenium stoste, i.e. a kind of probiotics product with probiotic bacterium and organoselenium dual function---probiotic bacterium live body selenium.
Adding accounts for the modified porous starch of gross weight 18～25% and 2～5% clinoptilolite powder in the probiotic bacterium live body selenium stoste that will cultivate again, and stirring and evenly mixing adopts Highspeedcentrifugingandsprayingdrier to carry out the flash boiling spray drying, makes the agent of probiotic bacterium live body selenium powder.
Probiotics probiotic bacterium live body selenium preparation of the present invention all has preferably impact to microbial population of animal intestinal tract, anti-oxidative effect and immunologic function.The bioavailability of probiotic bacterium live body selenium will be higher than inorganic selenium, easily utilized by the animal plant absorbing, and the organoselenium safety coefficient is high, can not cause detrimentally affect to human and environment.Its effect and to estimate its security reliable is used for biological preventing treatment disease and has a wide range of applications as the additional of probiotic bacterium and organoselenium.
Embodiment
By following examples the present invention is specifically described; it is important to point out that following examples are only for the present invention will be further described; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential adjustment to the present invention according to the content of foregoing invention.
Probiotics of the present invention---the preparation technology of probiotic bacterium live body selenium can do along with actual production conditions the variation of suitable increase or minimizing technique.
One, yeast (yeast rich in selenium) is cultivated
Saccharomyces Cerevisiae in S accharomyces cerevisiae.
Adopt commercially available yeast saccharomyces cerevisiae (Institute of Microorganism, Academia Sinica), its preserving number is CGMCC NO.0539, and depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center.
1, fermentative medium formula:
The total reducing sugar amount is 8～30% hydrolysis sugar; 1% starch; Ammonium sulfate 0.8%; Phosphoric acid 0.1%; Sodium selenite 0.3～1.2g/L; The pH of substratum is 4.5～7.5.
Starch hydrolyzate that can preferred 8%～22% total reducing sugar amount includes 0.4～1.2g/L sodium selenite, and the pH of substratum is 4.5～7.5.
2, fermentation culture conditions:
The condition of fermentation culture yeast rich in selenium is: the inoculum size of seed liquor is 10～20%, under 26～32 ℃ condition, and stir culture 14～72 hours.
3, the technological process of production:
Slant strains → liquid spawn → level liquid seed culture → → three grades of liquid seeds cultivation → ferment tank cultivation → zymophyte stostes of secondary liquid seeds cultivation → for subsequent use.
Concrete preparation process is as follows:
(1) slant strains: total sugar content is 8～18% to yeast saccharomyces cerevisiae (yeast rich in selenium) bacterial classification inoculation in containing, sodium selenite concentration is on the wort solid inclined-plane of 0.3～0.6g/L, after 24～72 hours, put into 4 ℃ of Refrigerator stores 28～32 ℃ of cultivations.
(2) liquid spawn: after the yeast rich in selenium bacterial classification activation of preserving, connect a garland cells in the triangular flask that the malt extract medium that the 150ml total sugar content is 8～22%, sodium selenite concentration is 0.4～0.8g/L is housed, in 26～32 ℃ of stir culture 14～28 hours, be liquid spawn.
(3) level liquid seed culture: be equipped with liquid spawn in the triangular flask of the Starch Hydrolysis liquid culture medium that 150 milliliters of total sugar contents are 8～22%, sodium selenite concentration is 0.6～1.2g/L by 30 of 10～15% inoculum size accesses, 26～32 ℃ of stir culture 14～28 hours, be the level liquid inoculum.
(4) the secondary liquid seeds is cultivated: the first order seed nutrient solution is equipped with by 10～15% inoculum size access 50 liters of total sugar contents are 8～22%, sodium selenite concentration is the fermentor tank of the Starch Hydrolysis liquid culture medium of 0.6～1.2g/L, 26～32 ℃ of stir culture 14～48 hours, be secondary liquid seeds culture.
(5) three grades of liquid seeds are cultivated: the secondary seed nutrient solution is equipped with by 10～15% inoculum size access 500 liters of total sugar contents are 8～22%, sodium selenite concentration is the fermentor tank of the Starch Hydrolysis liquid culture medium of 0.6～1.2g/L, 26～32 ℃ of stir culture 24～36 hours, be three grades of liquid seeds nutrient solutions.The living bacteria count of three grades of seed culture original bacteria liquids of gained S. cervisiae 〉=10
8/ ml, for subsequent use.
Two, photosynthetic bacterium is cultivated:
Rhodopseudomonas palustris Rhodopseudomonas palustris.
Adopt commercially available Rhodopseudomonas palustris (Institute of Microorganism, Academia Sinica), its preserving number is ACCC No.10649, and depositary institution is Chinese agriculture microbial strains preservation administrative center.
1, culture medium prescription
(1) seed culture medium: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, calcium chloride 0.3g, distilled water 1.0L, pH6.8～7.0.
(2) fermention medium:
Dipotassium hydrogen phosphate 0.78g, potassium primary phosphate 0.5g, sal epsom 0.2g, yeast extract paste 2.5g, sodium-chlor 0.5g, calcium chloride 0.05g, ammonium sulfate 0.05g, sodium-acetate 1.65g, Sodium Tetraborate 0.38g, ammonium molybdate 0.22g, water 1.0L, pH6.5～7.52.
2, preparation technology:
(1) seed slant culture: Rhodopseudomonas palustris is inoculated on the solid slant culture base, and culture temperature is between 26～30 ℃, and intensity of illumination is 3000～6000lux, and anaerobism was cultivated 7～10 days.
(2) the seed shaking table is cultivated: in liquid nutrient medium, temperature 32～34 degree were cultivated 36～48 hours, the acquisition seed culture fluid with the bacterial classification inoculation of slant culture.
(3) fermentor cultivation: it is 6.5～7.5 that the pH value of substratum is transferred to, use again 121 ℃ of high-temperature sterilizations 20 minutes, after cooling, access photosynthetic bacteria seed liquor 5～10%, 28～32 ℃ of culture temperature, intensity of illumination are 3000～6000lux, airtight cultivation 5～7 days, nutrient solution is by the colourless redness that becomes, and obtaining wine-colored liquid is photosynthetic bacterium original bacteria liquid (living bacteria count 〉=10
8/ ml).The former liquid state of Rhodopseudomonas palustris seed culture of gained saves backup.
Three, plant lactobacillus is cultivated
Plant lactobacillus Lactobacillus Plantarum.
Adopt commercially available plant lactobacillus (Institute of Microorganism, Academia Sinica), its preserving number is CGMCC NO.1.19, and depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center.
1, culture medium prescription:
(1) slant preservation substratum
Glucose 1.5%, yeast powder 1%, calcium carbonate 1.5%, tween-80 0.1%, agar 2%, 1.6% purpurum bromocresolis ethanolic soln 0.1%, pH6.8～7.2.
(2) seed culture medium
Glucose 0.5%, peptone 1%, extractum carnis 1%, sodium acetate 0.5%, diammonium hydrogen citrate 0.2%, tween-80 0.1%, potassium primary phosphate 0.2%, bitter salt 0.002%, anhydrous manganese 0.005%, calcium carbonate 1%, pH6.8～7.2.
(3) fermention medium
Sucrose 2.9%, yeast powder 3.1%, peptone 2.5%, sodium acetate 0.6%, dipotassium hydrogen phosphate 0.2%, ammonium dihydrogen citrate 0.2%, tween-80 0.2%, sal epsom 0.01%, manganous sulfate 0.01%, pH6.5～7.5.
2, fermentation culture conditions:
The inoculum size of seed liquor is 5～30%, under 32～37 ℃ condition, and stir culture 20～30 hours.
3, preparation technology's flow process:
Slant strains → level liquid seed culture → → three grades of liquid seeds cultivation → ferment tank cultivation → stostes of secondary liquid seeds cultivation → for subsequent use.
Concrete preparation process is as follows:
(1) slant strains is cultivated: bacterial classification inoculation on substratum solid inclined-plane, was cultivated 24～36 hours at 32～37 ℃.
(2) level liquid seed culture: slant strains 1～4 ring is linked in the triangular flask that 150～500 milliliters of substratum are housed, cultivated 24～36 hours at 32～37 ℃.
(3) the secondary liquid seeds is cultivated: the first order seed nutrient solution is equipped with the fermentor tank of 50 liters of substratum by 10～20% inoculum size access, cultivated 20～30 hours at 32～37 ℃.
(4) three grades of liquid seeds are cultivated: the secondary seed nutrient solution is equipped with in the fermentor tank of 500 liters of substratum by 10～20% inoculum size access, cultivated 20～30 hours at 32～37 ℃.Stop three grades of seed culture stoste (living bacteria count 〉=10 of fermentation → plant lactobacillus
8/ ml), for subsequent use.
Four, compound cultivation
Complex medium:
The total reducing sugar amount is that 8～30% hydrolysis sugar liquids, starch 0.1～1%, urea 0.2～0.25%, ammonium sulfate 0.4～0.5%, potassium primary phosphate 0.2～0.25%, sodium selenite 0.3～1.2g/L, pH are 6.5～7.5.
Seed culture fluid with S. cervisiae, Rhodopseudomonas palustris, three bacterial classification single culture of plant lactobacillus, the inoculum size access of (can preferred 10～20%) is equipped with in the fermentor tank of the liquid complex medium that 5000 liters of total reducing sugar amounts are 8～15%, sodium selenite concentration is 0.3～1.2g/L and is mixed compound cultivation by 10～30%, under 28～32 ℃ condition, intermittent stirring was cultivated 24～48 hours, and static fermentation is 48～96 hours again.Cultivation makes probiotic bacterium live body selenium stoste, namely gets a kind of probiotics finished product with probiotic bacterium and organoselenium dual function---probiotic bacterium live body selenium.
Add in the probiotic bacterium live body selenium stoste that also can further again cultivation be made and account for the modified porous starch of gross weight 18～25% and 2～5% clinoptilolite powder, abundant stirring and evenly mixing, adopt high speed centrifugation spraying flash dryer under 45～65 ℃ of conditions, the expansion drying of spraying makes the agent of probiotic bacterium live body selenium powder.
The probiotics with probiotic bacterium and organoselenium dual function by fermentation process production provided by the present invention---probiotic bacterium live body selenium liquid product:
Biological selenium (in Se) content 300～1200ppm;
Total viable count 〉=4～800,000,000/ml.
Solid phase prod (pulvis):
Biological selenium (in Se) content 1200～4800ppm;
Total viable count 〉=15～3,000,000,000/g.
The present invention's said " probiotic bacterium live body selenium " refers to utilize microbe fermentation method that inorganic selenium is converted into and contains the probiotics viable bacteria body probiotics of selenium.This kind microbial preparation integrates the selenoamino acid that contains of organoselenium tropina, viable bacteria body, active enzyme preparation and free state, be called " probiotic bacterium live body selenium probiotics ", it has three large functions of probiotics viable bacteria preparation, biological enzyme formulation, organoselenium concurrently, and cheap for manufacturing cost, can replace for animals, agricultural antibiotic class medicine and inorganic selenium additive, be the developing ecology Organic farming, Development and Production organic Se-rich functional health-care food is taken a firm foundation.
One of principal character of the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function of the present invention's development is that it contains profitable probliotics active somatic cell, active biological enzyme and organoselenium tropina.Wherein probiotic bacterium comprises yeast, milk-acid bacteria, photosynthetic bacteria and meta-bolites thereof.Active biological enzyme comprises proteolytic enzyme, amylase, lipase and cellulase.To be inorganic selenium and protein transform the mixture of the seleno-amino acids that forms, selenium polypeptide, seleno nucleic acid fragment through the microorganism live body to the organoselenium tropina.
Total viable count reaches 500,000,000/ml～2,000,000,000/ml in the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function of the present invention's development, wherein S. cervisiae (yeast rich in selenium) accounts for 30～50% of total viable count, Rhodopseudomonas palustris accounts for 10～20% of total viable count, and plant lactobacillus accounts for 30～50% of total viable count.Above-mentioned thalline ratio slightly changes because of the change of culture condition.
The probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function that the present invention is mentioned can be widely used in livestock and poultry cultivation, culture fishery, plant husbandry, industry of planting forest or fruit tress, Edible Fungi, herbal medicine cultivation, produces the organic Se-rich functional foodstuff.
Important technology of the present invention is characterized as the preparation method of the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function.
The bacterial classification that the present invention adopts is the microbial strains that registers on the books in Ministry of Agriculture's " allowing the fodder additives kind catalogue of use ", comprises S. cervisiae, Rhodopseudomonas palustris, three bacterial classifications of plant lactobacillus.All buy from Institute of Microorganism, Academia Sinica, stability, the security of its product are reliable.This product can directly come into the market and Application Areas, easily accepts the supervision and management of Government departments.
Probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function provided by the invention, with the obvious advantage:
(1) substitute for animals, agricultural antibiotic class medicine and inorganic selenium additive comprehensively, be the developing ecology Organic farming, the functional selenium food of Development and Production organic Se-rich injects new vitality for the ecological agriculture of current stagnation.
(2) the present invention adopts the common micro-organisms zymotechnique, can work as factory building by heliogreenhouse, and fermentation equipment is simple, less investment, and material cost is low, and product has significantly functional and price advantage and practicality.Be the innovative technology product that the peasant " affords, can use ", be conducive to promote, popularize.
(3) probiotic bacterium live body selenium probiotics provided by the invention, because the toxicity of selenium has dropped near nonpoisonous state, therefore can suitably improve the consumption in agricultural-food are produced, be that the organic Se-rich functional foodstuff provides material guarantee for producing the organic Se-rich agricultural-food in enormous quantities.
(4) method of employing probiotic bacterium live body selenium probiotics provided by the invention: raw material is extensive, simple to operate, fermentation period is short, is fit to scale operation and on the basis of the existing conditions no matter how simple and crude small serial production, have widely actual application prospect, and have great social benefit and huge economic benefit.
Effect of the present invention is topmost to be embodied in: along with applying of the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function, organic Se-rich functional foodstuff on the market will be enriched gradually, with benefiting more than 40 the serious countries and regions that lack selenium in the Chinese nation and the whole world, make positive contribution for promoting human health.21 century is bio-science century, it also is the splendid age of probiotics, allow little ecosphere and pharmaceutical field interpenetrate, mutually cooperation, take the research and development of functional factor modification core technology as point of penetration and breach, to promoting the scientific-technical progress of China's functional foodstuff, enhance the capability of independent innovation and the core competitiveness of industry, make contributions for developing better more functional probiotics.Promoting China's structure of agriculture and village economy strategical adjustment and solving the agriculture, rural areas and farmers problem to reach that the equal tool in aspect is of great significance to developing a circular economy, realize Sustainable development, build a harmonious society etc.
Claims (8)
1. the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function is characterized in that: comprise S. cervisiae, Rhodopseudomonas palustris and plant lactobacillus.
2. the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function according to claim 1, it is characterized in that: described S. cervisiae accounts for 30～50% of total viable count, described Rhodopseudomonas palustris accounts for 10～20% of total viable count, and described plant lactobacillus accounts for 30～50% of total viable count.
3. the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function according to claim 2, it is characterized in that: in the described probiotics, in Se, biological selenium content is 300～4800ppm; Total viable count 〉=400,000,000/ml.
4. the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function according to claim 3, it is characterized in that: described probiotics is liquid, in Se, biological selenium content is 300～1200ppm; Total viable count is 4～800,000,000/ml.
5. the probiotic bacterium live body selenium probiotics with probiotic bacterium and organoselenium dual function according to claim 3, it is characterized in that: described probiotics is solid, in Se, biological selenium content is 1200～4800ppm, and total viable count is 15～3,000,000,000/g.
6. the preparation method with probiotic bacterium live body selenium probiotics of probiotic bacterium and organoselenium dual function comprises the steps:
(1) preparation of yeast saccharomyces cerevisiae bacteria culture fluid gets three grades of seed culture fluids of S. cervisiae;
(2) preparation of Rhodopseudomonas palustris nutrient solution gets the Rhodopseudomonas palustris seed culture fluid;
(3) preparation of plant lactobacillus nutrient solution gets three grades of seed culture fluids of plant lactobacillus;
(4) with the seed culture fluid of S. cervisiae, Rhodopseudomonas palustris, three bacterial classification single culture of plant lactobacillus, carry out compound cultivation in the fermentor tank by the liquid complex medium that 10～30% inoculum size access total reducing sugar amount is 8～15%, sodium selenite concentration is 0.3～1.2g/L, under 28～32 ℃ condition, intermittent stirring was cultivated 24～48 hours, static fermentation is 48-96 hour again, cultivates to make liquid probiotic bacterium live body selenium probiotics.
7. the preparation method with probiotic bacterium live body selenium probiotics of probiotic bacterium and organoselenium dual function according to claim 6, it is characterized in that: described liquid probiotic bacterium live body selenium probiotics, add and account for the modified porous starch of gross weight 18～25% and 2～5% clinoptilolite powder, abundant stirring and evenly mixing, adopt high speed centrifugation spraying flash dryer under 45～65 ℃ of conditions, the expansion drying of spraying gets solid probiotic bacterium live body selenium probiotics.
8. the preparation method with probiotic bacterium live body selenium probiotics of probiotic bacterium and organoselenium dual function according to claim 6, it is characterized in that: described S. cervisiae accounts for 30～50% of total viable count, described Rhodopseudomonas palustris accounts for 10～20% of total viable count, and described plant lactobacillus accounts for 30～50% of total viable count.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103652329A (en) * | 2013-12-18 | 2014-03-26 | 大连金砣水产食品有限公司 | Compound probiotic biological agent |
| CN105166459A (en) * | 2015-08-07 | 2015-12-23 | 淮安正昌饲料有限公司 | Pig feed |
| CN105166458A (en) * | 2015-08-07 | 2015-12-23 | 淮安正昌饲料有限公司 | Fermented selenium-rich plant feed additive resistant to pig heat stress |
| CN105166485A (en) * | 2015-08-28 | 2015-12-23 | 上海普缇康生物技术有限公司 | Making method of dry dog food made through microorganism fermentation |
| CN106954640A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of method of utilization microbial control trees disease |
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| CN108486001A (en) * | 2018-03-13 | 2018-09-04 | 常州大学 | A kind of photosynthetic microbial inoculum of new and effective active selenium-rich and its preparation method and application for paddy growth |
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| CN113773149A (en) * | 2021-10-22 | 2021-12-10 | 武汉富田生物科技有限公司 | Novel fertilizer production process for improving selenium enrichment of crops |
| CN114606145A (en) * | 2022-04-22 | 2022-06-10 | 广东海纳川生物科技股份有限公司 | Saccharomyces cerevisiae micro-ecological preparation and application thereof |
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| CN114947002A (en) * | 2022-05-26 | 2022-08-30 | 西北工业大学 | Composite microecological preparation for feed and preparation method thereof |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103652329A (en) * | 2013-12-18 | 2014-03-26 | 大连金砣水产食品有限公司 | Compound probiotic biological agent |
| CN105166459A (en) * | 2015-08-07 | 2015-12-23 | 淮安正昌饲料有限公司 | Pig feed |
| CN105166458A (en) * | 2015-08-07 | 2015-12-23 | 淮安正昌饲料有限公司 | Fermented selenium-rich plant feed additive resistant to pig heat stress |
| CN105166485A (en) * | 2015-08-28 | 2015-12-23 | 上海普缇康生物技术有限公司 | Making method of dry dog food made through microorganism fermentation |
| CN106954640A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of method of utilization microbial control trees disease |
| CN106954639A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of preparation of complex microorganism bacterium solution and its application process |
| JP2020516699A (en) * | 2017-04-17 | 2020-06-11 | ジャンスー デイセバイオテック リミテッド | Tumor Chemotherapy Microbial Agents to Prevent Enterotoxicity |
| CN108486001A (en) * | 2018-03-13 | 2018-09-04 | 常州大学 | A kind of photosynthetic microbial inoculum of new and effective active selenium-rich and its preparation method and application for paddy growth |
| CN109349644A (en) * | 2018-10-30 | 2019-02-19 | 广州普维君健药业有限公司 | Probiotic composition, health food and its preparation method and application |
| CN111466503A (en) * | 2020-04-22 | 2020-07-31 | 江南大学 | Method for preparing selenium-rich enzyme beverage |
| CN112094779A (en) * | 2020-09-25 | 2020-12-18 | 安徽希普生物科技有限公司 | Compound microbial preparation containing bacillus subtilis |
| CN112094779B (en) * | 2020-09-25 | 2021-05-28 | 安徽希普生物科技有限公司 | Compound microbial preparation containing bacillus subtilis |
| CN112266882A (en) * | 2020-10-21 | 2021-01-26 | 重庆灵水生物科技有限公司 | Preparation method of micro-ecological probiotic preparation |
| TWI769644B (en) * | 2020-12-30 | 2022-07-01 | 陳俊宏 | Composite composition of clostridium butyricum, yeast and enzyme, oral composite including the same and application on liver protection |
| CN113773149A (en) * | 2021-10-22 | 2021-12-10 | 武汉富田生物科技有限公司 | Novel fertilizer production process for improving selenium enrichment of crops |
| CN114606145A (en) * | 2022-04-22 | 2022-06-10 | 广东海纳川生物科技股份有限公司 | Saccharomyces cerevisiae micro-ecological preparation and application thereof |
| CN114606145B (en) * | 2022-04-22 | 2023-10-13 | 广东海纳川生物科技股份有限公司 | Saccharomyces cerevisiae microecological preparation and application thereof |
| CN114947002A (en) * | 2022-05-26 | 2022-08-30 | 西北工业大学 | Composite microecological preparation for feed and preparation method thereof |
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