CN102172273B - Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice - Google Patents
Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice Download PDFInfo
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- CN102172273B CN102172273B CN2011100554259A CN201110055425A CN102172273B CN 102172273 B CN102172273 B CN 102172273B CN 2011100554259 A CN2011100554259 A CN 2011100554259A CN 201110055425 A CN201110055425 A CN 201110055425A CN 102172273 B CN102172273 B CN 102172273B
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Abstract
The invention provides a method for producing cordyceps militaris fermentation rice (cordyceps rice for short) by utilizing solid state fermentation of rice, and the method is characterized in that the rice is taken as a main fermentation substrate; and the cordyceps mycelia grows in the rice through a solid state fermentation manner so as to obtain the rice rich in the cordyceps mycelia. The method comprises the following steps: 1. preparing a cordyceps militaris liquid strain; 2. preparing a rice solid state fermentation culture medium; and 3. inoculating and fermenting. In the method, the medical effect of the cordyceps militaris is organically combined with the nutrition of the rice, thus the product contains various nutritive components and mineral elements, especially cordycepin and cordycepic acid, wherein, the content of the cordycepin is more than or equal to 600mg/100g, and the content of the cordycepic acid is more than or equal to 3g/100g, thereby improving the immunity of the organism, inhibiting tumours, preventing ageing, and satisfying the requirements of healthy eating of people.
Description
Technical field
The invention belongs to a kind of method of producing the fermentation rice, is a kind of method of utilizing the rice solid state fermentation to produce Cordyceps militaris fermentation rice specifically.
Background technology
Cordyceps militaris is a kind of medicinal fungi of preciousness, for a long time, and the treasure that always is considered as promoting longevity by people.Modern medicine proves, Cordyceps militaris has the artery sclerosis of preventing, improves immunity, antitumor, the anti-ageing multiple effect of waiting for a long time.
Being used at present the primary raw material of Development and Production Cordyceps militaris product mainly is tame fructification, but because of the fruiting bodies of cordyceps militaris mouthfeel relatively poor, can not directly eat, and fruiting bodies of cordyceps militaris belongs to medicine, can not in food, arbitrarily add, thereby so that there is certain limitation in the consumption of Cordyceps militaris product.And the fruiting bodies of cordyceps militaris formation cycle is long, and required labour intensity is large, has improved the cost of Cordyceps militaris product.The existence of these problems has restricted the development of Cordyceps militaris product and has popularized.
How can produce and be easy to Cordyceps militaris product edible and that digest and assimilate, simplify simultaneously production technology, reduce cost, be the common technical tasks of paying close attention to of present people.
Summary of the invention
The objective of the invention is the problem for prior art and product existence, a kind of method of utilizing the rice solid state fermentation to produce Cordyceps militaris fermentation rice is provided, make people can eat and digest and assimilate safely, easily the nutritional labeling of Cordyceps militaris, simultaneously, solve Cordyceps militaris product complex manufacturing, problem that cost is high.
The present invention seeks to be achieved through the following technical solutions: it is characterized in that: take rice as main fermentation substrate, the mode by solid state fermentation is grown cordyceps mycelium in rice, obtain being rich in the rice of cordyceps mycelium.Comprise the steps:
1, with after the activated cultivation of slant preservation bacterial classification, accesses in the semi-synthetic fluid nutrient medium, cultivate 5d～10d, namely get the Cordyceps militaris bacterial classification for 20 ℃～28 ℃.
2, the round of packing into after rice and water are mixed according to 4: 1～6: 1 weight ratio, 115 ℃～132 ℃ sterilization 15min～40min.Sterilization is cooled to normal temperature after finishing under cleaning condition, for subsequent use.
3, by 10%～15% inoculum concentration access Cordyceps militaris bacterial classification in the rice, under 20 ℃～28 ℃ conditions, after lucifuge is cultivated 5d～8d, change over to again under the illumination condition of 100lx～150lx and cultivate 15d～20d, finally natural drying at ambient temperature, namely obtain Cordyceps militaris fermentation rice.
After the activated cultivation of slant preservation bacterial classification, access in the semi-synthetic fluid nutrient medium, the primary raw material of semi-synthetic fluid nutrient medium has potato or wheat bran liquor, glucose, skimmed milk power, cultivate 5d～10d for 20 ℃～28 ℃, make the Cordyceps militaris bacterial classification, wherein the primary raw material of semi-synthetic fluid nutrient medium composition is expressed as potato or wheat bran liquor: glucose: skimmed milk power=1000: 20: 2 by weight.Wherein optimum culturing temperature is 25 ℃, and best incubation time is 7d.
The round of packing into after rice and water mixed according to 4: 1～6: 1 weight ratio, behind autoclave sterilization, cooling makes rice solid state fermentation substrate under cleaning condition.Wherein the optimum proportioning of rice and water is 5: 1, and best sterilising conditions is 121 ℃ of 35min, and round can be used fermentation tank (pond, cylinder, bag, bottle etc.).
Further, optimum temperature range is 23 ℃～25 ℃ in the step (3), and the Best Times that lucifuge is cultivated is 7d, and best incubation time is 15d under the illumination condition, and optimum illumination intensity is 110lx.
The present invention compared with prior art has many advantages and good effect:
1, pure fermented grain is rich in nutrition and is easy to edible and digests and assimilates.
Cordyceps militaris fermentation rice is to carry out solid state fermentation to form take rice as raw material, can cater to compatriots to the preference of cereal.By fermentation, the drug effect of Cordyceps militaris and the nutrition of rice can organically combine, the Cordyceps militaris fermentation Mi Hanyou multiple nutritional components and the mineral matter element that finally obtain, particularly be rich in cordycepin and cordycepic acid, cordycepin content 〉=600mg/100g wherein, cordycepic acid content 〉=3g/100g has effects such as improving immunity of organisms, improve microcirculqtory system, be antitumor.Exist because Cordyceps militaris fermentation rice is the form with the grain of rice, therefore, people can eat it easily as edible general rice.
2, production link has good security through strict monitoring.
The production of Cordyceps militaris bacterial classification is carried out in strict accordance with the sterile working rules; Being used for the liquid medium starting material of bacterial classification production and kind and the consumption of additive is to need strict control, and its quality will detect through strict; Solid state fermentation raw material-rice is through autoclave sterilization; Sweat is to need to get rid of miscellaneous bacteria and noxious pollutant interference.By the strict monitoring to each production link, make the cordyceps decimillimeter possess good security.
3, production technology is simple, easily grasps, and cost is low.
The present invention adopts the form of solid state fermentation, and therefore used fermentation substrate, can get rid of the interference of miscellaneous bacteria during the fermentation substantially through strict sterilization, more easily grasp than liquid state fermentation, and biochemical reactor designing requirement and expense is also low.Whole production procedure is divided into three link: make the Cordyceps militaris bacterial classification; Make the rice solid-state fermentation culture medium; Inoculation and fermentation.Three link can be realized automated job, can reduce cost of human resources.
The specific embodiment
The below illustrates the inventive method concrete example:
The present invention is a kind of method of utilizing the rice solid state fermentation to produce Cordyceps militaris fermentation rice, comprises the steps:
1, with slant preservation bacterial classification activation culture 7d on the PDA flat board, the bacterium piece access of beating cut-off footpath 0.5cm contain the 500mL triangle of 150mL potato glucose fluid nutrient medium bottled in, 25 ℃, 150r/min concussion are cultivated 7d, namely get the Cordyceps militaris bacterial classification.
2, get 50g rice and 10mL water and pack in the polypropylene plastics pocket of 20 * 22cm 121 ℃ of sterilization 35min into.Sterilization is cooled to normal temperature after finishing under cleaning condition, for subsequent use.
3, every bag of rice access 8mL Cordyceps militaris bacterial classification under 23 ℃～25 ℃ conditions, after lucifuge is cultivated 7d, continues to cultivate 15d under the 110lx intensity of illumination, takes off bag, and is natural drying at ambient temperature, namely obtains Cordyceps militaris fermentation rice.
Certainly, above-mentioned explanation is not to be restriction to invention, the present invention also be not limited to above-mentioned for example, those skilled in the art, in essential scope of the present invention, the variation of making, remodeling, interpolation or replacement all should belong to protection scope of the present invention.
Claims (3)
1. utilize the rice solid state fermentation to produce the method for Cordyceps militaris fermentation rice, it is characterized in that: take rice as main fermentation substrate, mode by solid state fermentation is grown cordyceps mycelium in rice, obtain being rich in the rice of cordyceps mycelium, comprises the steps:
(1), with after the activated cultivation of slant preservation bacterial classification, access in the semi-synthetic fluid nutrient medium, cultivate 5d～10d, namely get the Cordyceps militaris bacterial classification for 20 ℃～28 ℃;
(2), the round of packing into after rice and water mixed according to 4: 1～6: 1 weight ratio, 115 ℃～132 ℃ sterilization 15min～40min, sterilization is cooled to normal temperature after finishing under cleaning condition, for subsequent use;
(3), press 10%～15% access Cordyceps militaris bacterial classification of rice weight, under 20 ℃～28 ℃ conditions, after lucifuge is cultivated 5d～8d, change over to again under the illumination condition of 100lx～150lx and cultivate 15d～20d, natural drying at ambient temperature, namely obtain Cordyceps militaris fermentation rice, Cordyceps militaris fermentation rice exists with the form of the grain of rice;
The primary raw material of described semi-synthetic fluid nutrient medium has potato or wheat bran liquor, glucose, skimmed milk power, wherein the primary raw material of semi-synthetic fluid nutrient medium composition is expressed as potato or wheat bran liquor: glucose: skimmed milk power=1000: 20: 2 by weight.
2. method according to claim 1, it is characterized in that: wherein the proportioning of rice and water is 5: 1, and sterilising conditions is 121 ℃ of 35min, and round is fermentation tank.
3. method according to claim 1, it is characterized in that: the temperature range in the step (3) is 23 ℃～25 ℃, and the time that lucifuge is cultivated is 7d, and incubation time is 15d under the illumination condition, and intensity of illumination is 110lx.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100554259A CN102172273B (en) | 2011-03-09 | 2011-03-09 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
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| CN2011100554259A CN102172273B (en) | 2011-03-09 | 2011-03-09 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
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| CN102172273A CN102172273A (en) | 2011-09-07 |
| CN102172273B true CN102172273B (en) | 2013-02-20 |
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| CN102586048B (en) * | 2011-11-14 | 2014-01-15 | 尹志强 | Health care alcohol brewed with cordyceps sinensis funginite |
| CN102823806B (en) * | 2012-09-20 | 2013-09-25 | 王永显 | Method for producing fermented oatmeal using Cordyceps militaris |
| CN103960357A (en) * | 2013-02-06 | 2014-08-06 | 殷锋科 | Ecological transformation process for producing low-glycemic-index staple food by utilizing cordyceps militaris |
| CN103271281A (en) * | 2013-06-09 | 2013-09-04 | 食品行业生产力促进中心 | Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation |
| CN105018262A (en) * | 2014-04-15 | 2015-11-04 | 温州大学 | Cordyceps militaris draft beer and brewing process thereof |
| CN104718987A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis chenopodium quinoa |
| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104686202A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis sweet potatoes |
| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN104718989A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis dasheen |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
| CN105077028B (en) * | 2015-09-10 | 2018-10-16 | 即墨市第一职业中等专业学校 | A kind of preparation method of Cordceps militaris health-care rice |
| CN108771116A (en) * | 2018-05-22 | 2018-11-09 | 王长虹 | A kind of processing method of multi-functional composite rice |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
| CN109805270A (en) * | 2019-01-28 | 2019-05-28 | 江南大学 | A method of low albumen millet is produced using two-way solid state fermentation |
| CN110484404A (en) * | 2019-09-11 | 2019-11-22 | 河南科技学院 | A kind of Cordyceps militaris alcoholic beverage and its application |
| CN113133951B (en) * | 2020-01-19 | 2023-06-09 | 克一股份有限公司 | Production method for extracting rice protein extract from cordyceps militaris fermentation product and application of rice protein extract |
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| CN1078051C (en) * | 1998-07-21 | 2002-01-23 | 李健 | Method for preparing health-care food contg. chinese caterpillar fungus |
| CN1576362A (en) * | 2003-06-26 | 2005-02-09 | 薛丽贞 | Cordycepin food and cordycepin substrate and preparing process thereof |
| CN1944625B (en) * | 2006-10-30 | 2010-05-12 | 刘桢鋆 | Coterpillar fungus hypha cultivating method of rice solid fermenting for caterpillar fungus drink |
| CN101177665B (en) * | 2006-11-06 | 2010-09-15 | 张刚 | Industrial method for culturing north winter worm summer herb with high-content of cordycepin |
| CN101372671B (en) * | 2008-09-28 | 2010-09-29 | 鲁东大学 | Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof |
| CN101695257B (en) * | 2009-10-30 | 2011-03-30 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
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