CN114606145A - Saccharomyces cerevisiae micro-ecological preparation and application thereof - Google Patents
Saccharomyces cerevisiae micro-ecological preparation and application thereof Download PDFInfo
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Abstract
The invention provides a saccharomyces cerevisiae micro-ecological preparation and application thereof. The preservation number of the saccharomyces cerevisiae is GDMCC No. 62246; the liquid saccharomyces cerevisiae microbial inoculum after the saccharomyces cerevisiae fermentation treatment is mixed with isomaltooligosaccharide, xylooligosaccharide, glucose and molasses, a microecological preparation is obtained through post treatment, and the microecological preparation is applied to the preparation of animal feed, can exert the functions of efficiently enhancing the immunity and disease resistance of the organism, has excellent and stable activity in the organism, shows the balance of the microecologics in the intestinal tract of the organism, and has the advantages of few components, low raw material price and obvious effect when being applied to the preparation of the animal feed. In addition, in the scheme, the saccharomyces cerevisiae can easily absorb selenium components in the liquid culture medium, the selenium expression capacity is excellent, and the organism immunity effect of the follow-up preparation of the microecological preparation is further promoted.
Description
Technical Field
The invention relates to the field of preparation of microecologics, and particularly relates to a saccharomyces cerevisiae microecologic preparation and application thereof.
Background
Saccharomyces cerevisiae is also known as baker's yeast or budding yeast. Saccharomyces cerevisiae is the most widely related yeast to human, not only because it is traditionally used for making bread, steamed bread and other food and brewing wine, but also as a eukaryotic model organism in modern molecular and cellular biology, and its function is equivalent to prokaryotic model organism Escherichia coli. Saccharomyces cerevisiae is the most commonly used biological species in fermentation. The cells of Saccharomyces cerevisiae are spherical or ovoid, 5-10 μm in diameter. The reproduction method is budding reproduction. Because of the use of a large amount of antibiotics, pathogenic microorganisms generate drug resistance and drugs are remained in animal products, potential harm is caused to the health of human beings, and because the saccharomyces cerevisiae is added into livestock and poultry feed products, the digestion capacity, the immunity and the disease resistance of organisms are improved, so that the application of the saccharomyces cerevisiae to antibiotic substitute products also becomes a hot point.
However, impurities are easily introduced in the culture process of the saccharomyces cerevisiae in the prior art, and the impurity removal process can cause the problem of high production cost. In addition, the saccharomyces cerevisiae cultured by the prior art is unstable in activity, shows activity imbalance in different temperature environments, and has poor anti-mixed bacteria capability and poor reproductive capacity, so that the saccharomyces cerevisiae has poor effect in the application process.
Disclosure of Invention
Based on the above, in order to solve the problems that impurities are easily introduced in the saccharomyces cerevisiae culture process, the activity of the saccharomyces cerevisiae is unstable, the activity is unbalanced in different temperature environments, and the application effect is poor due to poor anti-infectious bacteria capability and poor reproductive capacity in the application process of the saccharomyces cerevisiae in the prior art, the invention provides the saccharomyces cerevisiae microecological preparation, which has the following specific technical scheme:
a Saccharomyces cerevisiae (Saccharomyces cerevisiae) with a preservation time of: no. 02/9 in 2022; the preservation unit is as follows: guangdong province culture Collection of microorganisms (GDMCC) with the address: building No. 59, building No. 5 of the first-furious Zhonglu 100 yard in Guangzhou city; the preservation number is: GDMCC No. 62246; the preservation form is as follows: vacuum freeze drying tube.
Saccharomyces cerevisiae with the preservation number of GDMCC No. 62246.
A saccharomyces cerevisiae micro-ecological preparation, which comprises the saccharomyces cerevisiae.
Further, the amount of the saccharomyces cerevisiae live bacteria is not less than 1 multiplied by 108CFU/g。
Further, the saccharomyces cerevisiae micro-ecological preparation is obtained by mixing a liquid saccharomyces cerevisiae microbial inoculum obtained by fermenting the saccharomyces cerevisiae with a carrier and carrying out post-treatment.
Further, the carrier is isomaltooligosaccharide, xylooligosaccharide, glucose and molasses.
Further, the proportion of the isomaltooligosaccharide, the xylooligosaccharide, the glucose and the molasses is 1-5:1-7:2-7:1-3 according to the mass ratio.
Further, the saccharomyces cerevisiae micro-ecological preparation also comprises compound vitamins.
Further, the fermentation treatment is divided into primary fermentation and secondary fermentation.
Further, the conditions of the primary fermentation are as follows: the primary fermentation temperature is 26-29 ℃, the stirring speed is 200-260rpm/min, the ventilation quantity is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the primary fermentation time is 16-20 h; the conditions of the secondary fermentation are as follows: the secondary fermentation temperature is 25-29 ℃, the stirring speed is 150-165rpm/min, the ventilation volume is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the secondary fermentation time is 18-25 h.
In addition, the application also provides an application of the saccharomyces cerevisiae microecological preparation, wherein the saccharomyces cerevisiae microecological preparation is added into the preparation of animal feed.
The saccharomyces cerevisiae is obtained by screening, has excellent activity stability, strong anti-infectious bacteria capability and strong reproductive capacity. The micro-ecological preparation containing the saccharomyces cerevisiae can exert the functions of efficiently enhancing the immunity and disease resistance of organisms, has excellent and stable activity in organisms, shows the balance of micro-ecology in intestinal tracts of the organisms, reduces the application of antibiotics, and has the advantages of few components, low raw material price and obvious effect when being applied to the preparation of animal feed. In addition, in the scheme, the saccharomyces cerevisiae can easily absorb selenium components in the liquid culture medium, the selenium expression capacity is excellent, and the organism immunity effect of the follow-up preparation of the microecological preparation is further promoted.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to embodiments thereof. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
In one embodiment of the present invention, the saccharomyces cerevisiae has a preservation number of GDMCC No.62246, and the preservation form is vacuum freeze drying tube preservation.
The Saccharomyces cerevisiae cell is oval, circular or cylindrical, has no flagellum and no movement, and has cell wall containing no cellulose, polysaccharides such as dextran, mannan, etc., and components such as protein, lipid and chitin, etc., and has rapid propagation, strong metabolism and many metabolites. In animal husbandry, the development and utilization of saccharomyces cerevisiae are also deepened, and the characteristics and distribution of saccharomyces cerevisiae contain a large amount of saccharomyces cerevisiae in the animal digestive tract, so that the saccharomyces cerevisiae can be separated from animal excrement.
Saccharomyces cerevisiae with the preservation number GDMCC No. 62246.
A saccharomyces cerevisiae micro-ecological preparation, which comprises the saccharomyces cerevisiae.
In one embodiment, the amount of the live bacteria of the saccharomyces cerevisiae is not less than 1 × 108CFU/g。
In one embodiment, the saccharomyces cerevisiae microecological preparation is obtained by mixing a liquid saccharomyces cerevisiae microbial inoculum obtained by fermenting saccharomyces cerevisiae with a carrier and performing post treatment.
In one embodiment, the carrier is isomaltooligosaccharide, xylooligosaccharide, glucose, and molasses.
In one embodiment, the ratio of isomaltooligosaccharide, xylooligosaccharide, glucose and molasses is 1-5:1-7:2-7:1-3 by mass.
In one embodiment, the saccharomyces cerevisiae micro-ecological preparation further comprises a vitamin complex.
In one embodiment, the fermentation process is divided into a primary fermentation and a secondary fermentation.
In one embodiment, the conditions of the primary fermentation are: the primary fermentation temperature is 26-29 ℃, the stirring speed is 200-260rpm/min, the ventilation quantity is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the primary fermentation time is 16-20 h; the conditions of the secondary fermentation are as follows: the secondary fermentation temperature is 25-29 ℃, the stirring speed is 150-165rpm/min, the ventilation volume is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the secondary fermentation time is 18-25 h.
In one embodiment, the medium of the primary fermentation is inoculated with 6-10% of liquid yeast species. The inoculum amount as used herein refers to the ratio of the volume of the liquid yeast species to the volume of the medium of the first fermentation stage after inoculation.
In one embodiment, the medium for the primary fermentation comprises the following components: 15-20g/L of agar, 2-6g/L of peptone, 6-10g/L of urea, 1-5g/L of sodium acetate, 1-4g/L of dipotassium hydrogen phosphate, 0.01-1 g/L of selenium and the balance of water.
In one embodiment, the primary fermentation medium is prepared by the following method: the components of the formula are uniformly mixed according to the content, and the mixture is sterilized for 20-30min at the temperature of 100-120 ℃.
In one embodiment, the saccharomyces cerevisiae after the primary fermentation is inoculated in the culture medium of the secondary fermentation according to the inoculation amount of 7-9%. The inoculum size as used herein refers to the ratio of the volume of the primary seed solution transferred to the volume of the secondary fermentation medium after inoculation.
In one embodiment, the formula of the culture medium for the secondary fermentation is as follows: 10-15g/L of molasses, 1-3g/L of urea, 1-6g/L of cane sugar, 1-2g/L of citric acid hydrogen diamine, 1-3g/L of dipotassium hydrogen phosphate, 0.03g/L-1g/L of selenium and the balance of water.
In one embodiment, the secondary fermentation medium is prepared by the following method: the components of the formula are uniformly mixed according to the content, and the mixture is sterilized for 20-30min at the temperature of 100-120 ℃.
In one embodiment, under the condition that the temperature is 24-28 ℃, the weight percentage of the mixture is 0.5-1.7: and adding the liquid saccharomyces cerevisiae microbial inoculum of 8-12 and the carrier into a stirring container, starting the stirring speed of the stirring container to be 100-300rpm/min, stirring for 20-60min, then carrying out freeze drying, and crushing until the mesh number is 60-120 meshes to obtain the saccharomyces cerevisiae micro-ecological preparation.
In one embodiment, under the condition that the temperature is 24-28 ℃, the weight percentage of the mixture is 0.5-1.7: 7-10:1-5 of the liquid saccharomyces cerevisiae microbial inoculum, the carrier and the compound vitamin are added into a stirring container, the stirring speed of the stirring container is started to be 100-300rpm/min, and after stirring for 20-60min, freeze drying is carried out, and the powder is crushed to 60-120 meshes, so as to obtain the saccharomyces cerevisiae micro-ecological preparation
In one embodiment, the saccharomyces cerevisiae micro-ecological preparation is added in the preparation of animal feed in the following amount: 0.5-3.5 g/Kg.
In addition, the application also provides a saccharomyces cerevisiae micro-ecological preparation applied to the preparation of animal feed.
The saccharomyces cerevisiae is obtained by screening, has excellent activity stability, strong anti-infectious bacteria capability and strong reproductive capacity. The micro-ecological preparation containing the saccharomyces cerevisiae can exert the functions of efficiently enhancing the immunity and disease resistance of organisms, has excellent and stable activity in organisms, shows the balance of micro-ecology in intestinal tracts of the organisms, reduces the application of antibiotics, and has the advantages of few components, low raw material price and obvious effect when being applied to the preparation of animal feed. In addition, in the scheme, the saccharomyces cerevisiae can easily absorb selenium components in the liquid culture medium, the selenium expression capacity is excellent, and the organism immunity effect of the follow-up preparation of the microecological preparation is further promoted.
Embodiments of the present invention will be described in detail below with reference to specific examples.
Example 1:
in the embodiment, the saccharomyces cerevisiae is obtained by extracting, separating, screening and purifying cow dung.
The cow dung is the dung of healthy cows, the dung is selected and needs to be frozen in liquid nitrogen for 1d, then the dung is placed at the temperature of 75 ℃ for storage, and a saccharomyces cerevisiae mixture is extracted and separated for later use.
Separation of yeasts 500 Saccharomyces cerevisiae strains, i.e.the above-mentioned Saccharomyces cerevisiae mixture, were selected from 100 fecal samples in this test.
Inoculating 10% of saccharomyces cerevisiae in the following formula components: culturing beef extract 10g, glucose powder 20g, L-selenium-methyl selenocysteine 0.05g, and water 1L in culture medium at 26 deg.C for 24h, if the culture medium becomes turbid, microscopic checking whether it is infected with bacteria, screening Saccharomyces cerevisiae capable of growing for the first time to obtain selenium-rich Saccharomyces cerevisiae, and standing at 75 deg.C for use.
Inoculating the selenium-enriched saccharomyces cerevisiae with the inoculation amount of 1% (volume ratio) obtained by the first screening into the following formula components: 0.01g of anhydrous copper sulfate, 10g of beef extract, 20g of glucose powder, 0.05g of L-selenium-methyl selenocysteine and 1L of water, and observing after culturing at 26 ℃ for 24h, if the culture medium becomes turbid, the strain is proved to be high-copper resistant. Then, whether the bacteria are infected or not is checked under a microscope. And screening the saccharomyces cerevisiae capable of growing for the second time, and placing at 75 ℃ for later use.
Inoculating the saccharomyces cerevisiae with the inoculation amount of 1% (volume ratio) obtained by the second screening to the components of the formula: 0.01g of zinc oxide, 10g of beef extract, 20g of glucose powder, 0.05g of L-selenium-methyl selenocysteine and 1L of water, and observing after culturing at 26 ℃ for 24h, if the culture medium becomes turbid, the strain is proved to be high-zinc resistant. Then, whether the bacteria are infected or not is checked under a microscope. And thirdly, screening the saccharomyces cerevisiae capable of growing, and placing the saccharomyces cerevisiae at 75 ℃ for later use.
Inoculating the saccharomyces cerevisiae with the inoculation amount of 1% (volume ratio) obtained by the third screening to the components of the formula: 8g of vitamin complex, 10g of beef extract, 20g of glucose powder, 0.05g of L-selenium-methyl selenocysteine and 1L of water, and observing after culturing at 26 ℃ for 24h, if the culture medium becomes turbid, the strain is suitable for survival in the vitamin complex environment. Then, whether the bacteria are infected or not is checked under a microscope. And screening the saccharomyces cerevisiae capable of growing for the fourth time, and placing at 75 ℃ for later use.
The Saccharomyces cerevisiae selected by the method is preserved in Guangdong province microorganism culture collection center at No. 09.02/2022, and the preservation number is GDMCC No. 62246.
Example 2:
preparing a culture medium for primary fermentation: the formula of the culture medium for the first-stage fermentation comprises the following components: agar 10g, peptone 2g, urea 10g, sodium acetate 5g, dipotassium hydrogen phosphate 8g, selenium 0.01g, and water 1L, mixing well, sterilizing at 120 deg.C for 30 min.
Preparing a culture medium for secondary fermentation: the formula of the culture medium for secondary fermentation is as follows: 15g of molasses, 1-3g/L of urea, 1g of sucrose, 2g of diammonium hydrogen citrate, 1g of dipotassium hydrogen phosphate and 1L of selenium 0.03g of water, uniformly mixing, and sterilizing at 100 ℃ for 20 min.
The preparation method of the saccharomyces cerevisiae micro-ecological preparation comprises the following steps:
A1. the selected Saccharomyces cerevisiae with the preservation number of GDMCC No.62246 in example 1 was used as a strain, and 7% (volume ratio) of the strain was inoculated into a liquid culture medium and shake-cultured at 28 ℃ for 18 hours to obtain a shake flask liquid strain.
A2. Transferring the shake flask liquid strain to the culture medium of the first-stage fermentation at 6% (volume ratio), and preparing into first-stage seed liquid at a fermentation temperature of 26 ℃, a stirring speed of 250rpm/min, a ventilation rate of 1.0V/V.min, a dissolved oxygen concentration of not less than 40% and a fermentation time of 16 h;
A3. transferring the primary seed liquid into a culture medium of the secondary fermentation in an inoculation amount of 8% (volume ratio), and obtaining a liquid saccharomyces cerevisiae microbial inoculum at a fermentation temperature of 25 ℃, a stirring speed of 150rpm/min, a ventilation volume of 1.2V/V.min, a dissolved oxygen concentration of not less than 40% and a fermentation time of 20 h;
A4. mixing isomaltooligosaccharide, xylooligosaccharide, glucose and molasses in a mass ratio of 1:6:3:1 to obtain a carrier;
A5. under the condition of 24 ℃, mixing the components in percentage by mass as follows: and 5, adding the liquid saccharomyces cerevisiae microbial inoculum and the carrier into a stirring container, starting the stirring speed of the stirring container to be 100rpm/min, stirring for 20min to obtain a mixture, then performing freeze drying, and crushing to obtain the saccharomyces cerevisiae micro-ecological preparation with the mesh number of 60.
The preparation method is simple, the saccharomyces cerevisiae with good activity is easy to obtain, and the saccharomyces cerevisiae shows high stability and strong reproductive capacity. In addition, the saccharomyces cerevisiae is not easy to generate mixed bacteria in the culture process, is rich in selenium and can obtain the saccharomyces cerevisiae with the purity of 99.6 percent.
Example 3:
preparing a liquid culture medium: the formula comprises the following components: 10g of glucose powder, 0.01g of L-selenium-methyl selenocysteine and 1L of water are evenly mixed and sterilized for 20min at the temperature of 120 ℃.
Preparing a culture medium for primary fermentation: the formula of the primary fermentation medium comprises the following components: agar 10g, peptone 2g, urea 10g, sodium acetate 5g, dipotassium hydrogen phosphate 8g, selenium 0.02g, and water 1L, mixing well, sterilizing at 120 deg.C for 30 min.
Preparing a culture medium for secondary fermentation: the formula of the secondary fermentation medium is as follows: 15g of molasses, 3g of urea, 6g of cane sugar, 2g of diamine hydrogen citrate, 3g of dipotassium hydrogen phosphate, 0.04g of selenium and 1L of water, uniformly mixing, and sterilizing at 120 ℃ for 30 min.
The preparation method of the saccharomyces cerevisiae micro-ecological preparation comprises the following steps:
A1. taking the saccharomyces cerevisiae with the screened preservation number of GDMCC No.62246 in the example 1 as a strain, inoculating the saccharomyces cerevisiae with the inoculation amount of 5% (volume ratio) into a liquid culture medium, and carrying out shake culture at 28 ℃ for 18h to obtain a shake flask liquid strain;
A2. transferring the shake flask liquid strain to the culture medium of the first-stage fermentation at an inoculation amount of 10% (volume ratio), and preparing a first-stage seed solution at a fermentation temperature of 28 ℃, a stirring speed of 260rpm/min, a ventilation rate of 1.2V/V.min, a dissolved oxygen concentration of not less than 40% and a fermentation time of 20 h;
A3. transferring the primary seed liquid into a culture medium of the secondary fermentation in an inoculation amount of 7% (volume ratio), and obtaining a liquid saccharomyces cerevisiae microbial inoculum at a fermentation temperature of 29 ℃, a stirring speed of 170rpm/min, a ventilation volume of 1.2V/V.min, a dissolved oxygen concentration of not less than 40% and a fermentation time of 25 h;
A5. mixing the raw materials in a mass ratio of 1:6: 5:1, mixing isomaltooligosaccharide, xylooligosaccharide, glucose and molasses to obtain a carrier;
A6. under the condition of 28 ℃, mixing the components in percentage by mass as 1.7: 10, adding the liquid saccharomyces cerevisiae microbial inoculum and the carrier into a stirring container, starting the stirring speed of the stirring container to be 300rpm/min, stirring for 60min, then carrying out freeze drying, and crushing to 120 meshes to obtain the saccharomyces cerevisiae micro-ecological preparation.
Example 4:
preparing a liquid culture medium: the formula comprises the following components: 15g of glucose powder, 0.03g of L-selenium-methyl selenocysteine and 1L of water are uniformly mixed and sterilized for 30min at the temperature of 120 ℃.
Preparing a culture medium for primary fermentation: the formula of the primary fermentation medium comprises the following components: 15g of agar, 5g of peptone, 8g of urea, 3g of sodium acetate, 7g of dipotassium hydrogen phosphate, 0.03g of selenium and 1L of water, uniformly mixing, and sterilizing at 110 ℃ for 25 min.
Preparing a culture medium for secondary fermentation: the formula of the secondary fermentation medium is as follows: 12g of molasses, 2g of urea, 4g of cane sugar, 1g of diamine hydrogen citrate, 2g of dipotassium hydrogen phosphate and 0.03g of water are uniformly mixed and sterilized for 25min at 110 ℃.
The preparation method of the saccharomyces cerevisiae micro-ecological preparation comprises the following steps:
A1. the Saccharomyces cerevisiae with the preservation number of GDMCC No 62246 screened in example 1 is used as a strain, the Saccharomyces cerevisiae with the inoculation amount of 7 percent (volume ratio) is inoculated in a liquid culture medium, and shaking culture is carried out for 18h at 28 ℃ to obtain a shake flask liquid strain.
A2. Transferring the shake flask liquid strain to the culture medium of the first-stage fermentation at an inoculation amount of 8% (volume ratio), and preparing a first-stage seed solution at a fermentation temperature of 28 ℃, a stirring speed of 255rpm/min, a ventilation rate of 1.0V/V.min, a dissolved oxygen concentration of not less than 40% and a fermentation time of 18 h;
A3. transferring the primary seed liquid into a culture medium of the secondary fermentation according to the inoculation amount of 7% (volume ratio), and preparing the liquid saccharomyces cerevisiae microbial inoculum at the fermentation temperature of 28 ℃, the stirring speed of 160rpm/min, the ventilation volume of 1.1V/V.min, the dissolved oxygen concentration of not less than 40% and the fermentation time of 23 h;
A4. mixing isomaltooligosaccharide, xylooligosaccharide, glucose and molasses in a mass ratio of 3:7:2:3 to obtain a carrier;
A5. under the condition of 25 ℃, mixing the components in percentage by mass as follows: and 8, adding the liquid saccharomyces cerevisiae microbial inoculum and the carrier into a stirring container, starting the stirring speed of the stirring container to be 200rpm/min, stirring for 40min, then carrying out freeze drying, and crushing to 100 meshes to obtain the saccharomyces cerevisiae micro-ecological preparation.
Application test example 1:
and (3) detecting the liquid saccharomyces cerevisiae microbial inoculum in the embodiment 3 and detecting the prepared saccharomyces cerevisiae microecological preparation.
Reference document GB5009.93-2010 determination of selenium in food for a method for detecting the content of selenium in a microecological preparation.
In the liquid saccharomyces cerevisiae bacterial agent in the embodiment 3, 2.6g of dry bacteria can be obtained in each liter of the liquid saccharomyces cerevisiae bacterial agent, and the content of the total selenium obtained by detection in the obtained dry bacteria is 50398 mug/g. The culture method in the scheme can ensure higher activity and reproductive capacity of the saccharomyces cerevisiae, and simultaneously can obtain and apply the saccharomyces cerevisiae with high selenium content.
The liquid saccharomyces cerevisiae microbial inoculum is applied to the preparation of the microecological preparation, and the content of the total selenium is 50137 mu g/g in 15.8g of the dried microecological preparation through detection, which indicates that the content of the selenium in the microecological preparation can be ensured by applying the liquid saccharomyces cerevisiae microbial inoculum to the preparation of the microecological preparation.
Application test example 2:
the probiotic prepared in example 4 was applied to the preparation of animal feed, weaned piglets were selected as test subjects in this application test, 3 treatment groups were selected, and labeled treatment group 1, treatment group 2 and treatment group 3, each treatment group having 5 replicates, each having 10 piglets, and a control group was set. The test period is 60 days, the whole process is free to feed and drink water, and after the test is finished, 5 butchering heads are selected from each group to take the longissimus dorsi to determine the meat quality index.
The addition amount of the microecologics in the basal ration of the piglets is as follows: 0.5-3.5 g/Kg.
The formulation table of the piglet diet in application test example 1 is shown in table 1 below.
Table 1:
the preparation method of the piglet diet in the table 1 comprises the following steps: adding the components into a stirring kettle according to a formula, adding a certain amount of water, stirring and decocting for 20-120 min at the temperature of 60-100 ℃ and under the pressure of 1.2-5 MPa, concentrating to be viscous, and cooling to room temperature.
The average daily gain, average daily feed intake and feed-weight ratio were calculated by feeding the piglet diets in table 1 to the corresponding treatment groups, respectively, and the results are shown in table 2 below.
Table 2:
as can be seen from the data analysis in Table 2, the addition of a certain amount of the microecologics to the ration of the piglets in the treatment group is helpful for improving the weight gain of the piglets, but the average daily feed intake is reduced, and the weight gain of the piglets can still be ensured on the premise of less daily ration feeding of the piglets.
Slaughtering and taking the longissimus dorsi to determine the meat quality index, wherein the crude protein is determined by adopting a Dumas combustion method; muscle fat was measured by soxhlet extraction; l (lightness of flesh), a (redness) and b (yellowness) were measured using a colorimeter and the recordings are shown in table 3 below.
Table 3:
as can be seen from the analysis in table 3, the piglets in the treated group were superior to the control group in the values of crude protein, muscle fat, and flesh color brightness. The microecological preparation added in the scheme is beneficial to improving the pork quality, does not need to increase the growth period and change the existing feeding mode, and achieves better production performance.
Relevant morbidity rates were recorded throughout the test period observed for piglets in application test 1 and the structural record is shown in table 4 below.
Table 4:
| group of | Control group | Treatment group 1 | Treatment group 2 | Treatment group 3 |
| Incidence rate/%) | 8 | 0 | 0 | 0 |
As can be seen from the data analysis in table 4, the incidence of the piglets in the treatment group is low during the feeding process, wherein the incidence of the piglets in the treatment group 1, the treatment group 2 and the treatment group 3 is 0, but the incidence of the piglets in the control group is 8%, which indicates that the addition of a certain amount of the probiotics to the ration can effectively improve the body immunity of the piglets and reduce the incidence of the piglets.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. Saccharomyces cerevisiae with the preservation number GDMCC No. 62246.
2. A saccharomyces cerevisiae microecological formulation comprising the saccharomyces cerevisiae according to claim 1.
3. The saccharomyces cerevisiae micro-ecological preparation according to claim 2, wherein the amount of the saccharomyces cerevisiae live bacteria is not less than 1 x 108CFU/g。
4. The saccharomyces cerevisiae micro-ecological preparation according to claim 3, wherein the saccharomyces cerevisiae micro-ecological preparation is obtained by mixing a liquid saccharomyces cerevisiae microbial agent obtained by fermenting saccharomyces cerevisiae with a carrier and performing post-treatment.
5. The saccharomyces cerevisiae micro-ecological formulation according to claim 2, wherein the carrier is isomalto-oligosaccharide, xylose-oligosaccharide, glucose and molasses.
6. The saccharomyces cerevisiae micro-ecological preparation according to claim 5, wherein the ratio of the isomaltooligosaccharide, the xylooligosaccharide, the glucose and the molasses is 1-5:1-7:2-7:1-3 by mass ratio.
7. The saccharomyces cerevisiae micro-ecological formulation according to claim 6, wherein said saccharomyces cerevisiae micro-ecological formulation further comprises a vitamin complex.
8. The saccharomyces cerevisiae micro-ecological preparation according to claim 7, wherein the fermentation treatment is divided into a primary fermentation and a secondary fermentation.
9. The saccharomyces cerevisiae micro-ecological preparation according to claim 8, wherein the conditions of the primary fermentation are: the primary fermentation temperature is 26-29 ℃, the stirring speed is 200-260rpm/min, the ventilation quantity is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the primary fermentation time is 16-20 h; the conditions of the secondary fermentation are as follows: the secondary fermentation temperature is 25-29 ℃, the stirring speed is 150-165rpm/min, the ventilation volume is 1.0-1.2V/V.min, the dissolved oxygen concentration is not lower than 40 percent, and the secondary fermentation time is 18-25 h.
10. Use of a saccharomyces cerevisiae micro-ecological preparation, characterized in that it is added to an animal feed as described in any of claims 1 to 9.
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| CN111826293A (en) * | 2020-09-07 | 2020-10-27 | 广东海纳川生物科技股份有限公司 | Saccharomyces cerevisiae micro-ecological preparation and application thereof |
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