CN110317753A - A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application - Google Patents
A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application Download PDFInfo
- Publication number
- CN110317753A CN110317753A CN201910567038.XA CN201910567038A CN110317753A CN 110317753 A CN110317753 A CN 110317753A CN 201910567038 A CN201910567038 A CN 201910567038A CN 110317753 A CN110317753 A CN 110317753A
- Authority
- CN
- China
- Prior art keywords
- bacillus megaterium
- microbial inoculum
- liquid fermentation
- bacillus
- fermentation process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Sustainable Development (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of bacillus megaterium microbial inoculum, liquid fermentation process and its applications.The bacillus megaterium microbial inoculum is by deposit number: the bacillus megaterium access ingredient and proportion of GMCC No:1259 are as follows: the medium liquid fermentation of sucrose (0.5%), cornstarch (0.8%), soybean cake powder (3%), corn starch (0.8%), peptone (0.5%), calcium carbonate (0.05%), dipotassium hydrogen phosphate (0.08%), magnesium sulfate (0.13%), sodium chloride (0.1%), zinc sulfate (0.07%), potassium nitrate (0.01%) is made.The present invention substantially increases the production efficiency of bacillus megaterium 1259, increases yield, and method is simple.Bacillus megaterium BM1259 microbial inoculum is added in Cow product, in daily ration and can significantly improve milk cow production performance, is promoted the fermentation of cud and is reduced the loss of nitrogen in excreta.
Description
Technical field
The invention belongs to microbe additive fermenting and producing field, in particular to a kind of bacillus megaterium microbial inoculum, liquid
Zymotechnique and its application.
Background technique
It is nitrogen discharged with the continuous improvement of China's livestock and poultry breeding industry intensive degree and a large amount of investments of high nutrition feed
Caused by problem of environmental pollution constantly aggravating.Firstly, the excessive nitrogen discharged formation greenhouse gases (N into air2) and stench O
Gas (NH3), wherein foul gas not only influences breeding performonce fo animals, also can cause adverse effect to the health of keeper.
Be discharged into rivers secondly, excessive nitrogen enters fecaluria and cause water eutrophication, in addition can in the form of nitrate nitrogen and nitrite nitrogen into
Enter surface and ground water, threatens drinking water safety.Furthermore excessive nitrogen aggravates the negative of Soil Nitrogen by excrement returning to the field mode
Lotus.In addition, protein is feedstuff costly, and the excessive discharge of nitrogen is feed in fact from the point of view of production cost angle
The waste of resource and cost.Therefore, how to effectively control it is nitrogen discharged in Production of Livestock and Poultry be problem in the urgent need to address.
Currently, from the angle of livestock and poultry diet nutrition regulation, it is nitrogen discharged in Production of Livestock and Poultry on how to effectively control
Research is mainly around in terms of following two.On the one hand, the content of nitrogen in fecaluria is reduced by regulating and controlling the protein level of daily ration.Day
Crude protein content is related to the release altitude of ammonia in fecaluria in grain, increases crude protein content and the unbalanced daily ration of albumen in daily ration
The release of nitrogen can all be can increase.It is noted that although the reduction of Different Dietary Protein Levels can be significantly reduced in excreta
The content of nitrogen, but be sometimes also that cost is reduced to production performance.On the other hand, probiotics, enzyme preparation, plant extract
The application of the effectively feed addictive such as object, it is possible to reduce the discharge of nitrogen.Wherein, bacillus is added as optimal microorganism
Agent.Bacillus megaterium (Bacillus megaterium 1259, BM1259) is one plant for separating, extracting from soil
Bacterial strain with unique effects such as deodorizations, in Chinese microorganism strain collection preservation, preservation registration number is GMCC No
1259.The characteristic that feeding animals not only have the function of other feeding bacillus and safety is good, stability is good, but also to reduction
The effect of animal excrements stench is significant.
Bacillus megaterium has been widely used in the production of biological organic fertilizer, has been reported that and elaborates bacillus megaterium
BM1259 is as a kind of feed addictive to the positive influence of animal productiong.However, the liquid of bacillus megaterium BM1259 is sent out
Ferment technique has not been reported.
Summary of the invention
In order to overcome drawbacks described above, nitrogen discharged animal and fowl fodder microorganism can be reduced the purpose of the present invention is to provide a kind of
Liquid fermentation process and its application of additive-bacillus megaterium microbial inoculum and bacillus megaterium.
In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention are as follows:
A kind of bacillus megaterium microbial inoculum, by deposit number: the bacillus megaterium of GMCC No:1259 access ingredient and
Proportion are as follows: sucrose 0.5%, cornstarch 0.8%, soybean cake powder 3%, corn starch 0.8%, peptone 0.5%, calcium carbonate
0.05%, dipotassium hydrogen phosphate 0.08%, magnesium sulfate 0.13%, sodium chloride 0.1%, zinc sulfate 0.07%, potassium nitrate 0.01%
Culture medium is made through liquid fermentation；Percentage is mass percent；Wherein, in addition to mentioned component in culture medium, surplus is that water (adds
Entering water makes mass percent summation 100%).
The present invention also provides a kind of liquid fermentation process of bacillus megaterium microbial inoculum, include the following steps:
1) take deposit number: the bacillus megaterium of GMCC No:1259 is inoculated in thallus rejuvenation fluid nutrient medium, 30~
34 DEG C, pH6~9,180rpm cultivate 36~40h, obtain the bacteria suspension that light absorption value OD is 1.0~1.2 under 600nm；
2) liquid fermentation medium is poured into fermentor sterilizing；The ingredient and proportion of liquid fermentation medium are as follows: sucrose
0.5%, cornstarch 0.8%, soybean cake powder 3%, corn starch 0.8%, peptone 0.5%, calcium carbonate 0.05%, phosphoric acid hydrogen
Dipotassium 0.08%, magnesium sulfate 0.13%, sodium chloride 0.1%, zinc sulfate 0.07%, potassium nitrate 0.01%；
3) will be by bacterial suspension inoculation that step 1) prepares in liquid fermentation medium, bacterial suspension inoculation amount is liquid hair
1.0~5.0%v/v of ferment culture volume, is passed through filtrated air, and 33 ± 1 DEG C, 40~46h of 180rpm fermented and cultured；It fermented
Cheng Zhong is sampled microscopy every 6 hours, observes bacterium form and bacillus exfoliation situation, the gemma to 80% or more microscopy
When falling off, fermentation is terminated；
4) after fermentation terminates, fermentation liquid is centrifuged, collects thallus；The thallus of collection is placed in 45 DEG C of baking oven low temperature drying；
It is crushed after drying, bacillus megaterium BM1259 microbial inoculum is made.
The viable count of manufactured bacillus megaterium BM1259 microbial inoculum reaches 10 in step 4)10cfu/g.It is crushed in step 4)
The mesh number of the grinding screen of Shi Caiyong is 200 mesh.
Purposes and bacillus megaterium the present invention also provides bacillus megaterium microbial inoculum as additive for farm animal feed
The liquid fermentation process of microbial inoculum is preparing the purposes in additive for farm animal feed.
The present invention also provides a kind of cow feed additives, it is with deposit number: the huge gemma of GMCC No:1259
Bacillus fermentation is made.10~15g bacillus megaterium BM1259 microbial inoculum, milk cow basal diet are added in milk cow in lactation period daily ration
Group becomes corn silage (43%), corn (15.83%), soybean skin (5.8%), corn tabletting (5.72%), DDGS
(5.68%), beet pulp (4%), clover (4%), dregs of beans (3.52%), cottonseed (3%), Cottonseed Meal (2.86%), maize germ
The dregs of rice (2.5%), brewex's grains (2.46%), calcium monohydrogen phosphate (0.44%), calcium carbonate (0.35%), sodium bicarbonate (0.33%), salt
(0.22%), premix (0.18%) and yeast culture (0.11%).
Compared with the existing technology, advantages of the present invention:
The method of bacillus megaterium microbial inoculum liquid fermentation of the present invention, the shortcomings that overcoming solid fermentation low output,
Improve BM1259 production efficiency and yield.In addition, the supernatant that centrifugation generates, which can also be used for later period animal, takes orally opening for liquid product
Hair research, non-pollutant discharge realize clean manufacturing.The present invention is conducive to bacillus megaterium BM1259 and realizes that high-volume is raw
It produces, meets the needs of market is to animal and fowl fodder micro-ecological additive, may advantageously facilitate with China's independent intellectual property right, steady
Fixed, safe, green probiotics research and development, are also beneficial to develop china natural resources economizing type, environmentally friendly, " fish and bear's paw
Can get both " intensive high efficiency Production of Livestock and Poultry.
Using deposit number of the present invention: the additive for farm animal feed of the bacillus megaterium preparation of GMCC No:1259, it can be straight
It connects and makes an addition in livestock and poultry diet, livestock and poultry production performance not only can be improved, improve intestinal health, and can be by reducing livestock and poultry
The excessive discharge of nitrogen is to alleviate Production of Livestock and Poultry pollution on the environment.
Bacillus megaterium microbial inoculum liquid fermentation agent is applied to ruminant (milk cow) for the first time by the present invention, and is used as anti-
Hay animal diets additive.The digestive system of milk cow (ruminant) is different from general poultry.This fermentation process later period is dried
Cheng Caiyong low temperature drying can promote the spore of the bacillus to continue to generate in drying stage, and then improve rate containing spore.
Detailed description of the invention
The homologous comparison of Fig. 1 bacillus megaterium BM1259 16S rRNA sequence；
Degradation rate of Fig. 2 bacillus megaterium BM1259 to various concentration ammonia nitrogen.
Specific embodiment
Above content of the invention is described in further detail below by way of example forms, but not to limit of the invention
System.The scope of the present invention is determined with core content according to claims.
Liquid fermentation process of the present invention is specific as follows:
A, take deposit number: the bacillus megaterium of GMCC No:1259 is inoculated in equipped with thallus rejuvenation fluid nutrient medium (ox
Meat extract peptone) triangular flask in, 30~34 DEG C, pH6~9,180rpm cultivate 36~40h, obtaining light absorption value OD under 600nm is
1.0~1.2 bacteria suspension.
B, after the sterilizing of Zymolysis Equipment pipeline, prepared liquid fermentation medium is poured into fermentor sterilizing.Wherein, liquid
The ingredient and proportion of fermentation medium are as follows: sucrose (0.5%), cornstarch (0.8%), soybean cake powder (3%), corn starch
(0.8%), peptone (0.5%), calcium carbonate (0.05%), dipotassium hydrogen phosphate (0.08%), magnesium sulfate (0.13%), sodium chloride
(0.1%), zinc sulfate (0.07%), potassium nitrate (0.01%).
C, after cooling is completed in sterilizing, the bacterial suspension inoculation prepared by step a is trained in bacillus megaterium liquid fermentation
It supports in base, inoculum concentration is 1.0~5.0% (v/v) of culture volume, is passed through filtrated air, 33 ± 1 DEG C, 180rpm fermentation is trained
Support 40~46h.In fermentation process, it is sampled microscopy every 6 hours, bacterium form and bacillus exfoliation situation are observed, to mirror
When the bacillus exfoliation of 80% or more inspection, fermentation is terminated.
D, after fermentation terminates, fermentation liquid is passed through tube centrifuge and is centrifuged, collect thallus.The thallus of collection is placed in 45
DEG C baking oven low temperature drying.It is crushed after drying, the mesh number of grinding screen is 200 mesh, and bacillus megaterium BM1259 bacterium is made
Agent, viable count reach 1010cfu/g。
Experimental material is the bacterium powder produced by aforesaid liquid zymotechnique.
The identification of the bacillus megaterium BM1259 of the present invention of embodiment 1
According to the DNA sequence dna design primer for having obtained bacterial strain BM1259, amplification sequencing is carried out to its 16S rRNA.Obtain sequence
It is compared with the 16S rRNA of strains other in ncbi database, as the result is shown BM1259 and bacillus megaterium B.megaterium
The homology of ATCC14581 is higher, identifies that BM1259 is bacillus megaterium kind (Fig. 1).
2 shake flask test of embodiment: influence of the bacillus megaterium BM1259 to ammonia nitrogen degradation
Take deposit number: the bacillus megaterium of GMCC No:1259 is inoculated in equipped with thallus rejuvenation fluid nutrient medium (beef
Cream peptone) triangular flask in, 30~34 DEG C, 180rpm cultivate 18~20h.By 10% inoculum concentration, by the bacterium solution after activation
It accesses in the ammonia nitrogen culture medium of different initial concentrations, 7.0,32 DEG C of shaking table cultures of pH value are for 24 hours.After culture, ammonia nitrogen concentration is measured,
Ammonia nitrogen degradation rate is calculated, determines bacillus megaterium BM1259 to the degradation capability of various concentration ammonia nitrogen.As shown in Fig. 2, huge
Bacillus BM1259 reaches 80% or more to the ammonia nitrogen degradation rate of various concentration (25~300mg/L).
3 zoopery of embodiment: bacillus megaterium BM1259 is added in daily ration to milk cow in lactation period production performance, cud
Fermentation and the influence of biochemical indicator
24 outputs of milk, lactation age in days, china holstein cows similar in parity are selected, is randomly divided into 4 groups, every group 6,
In daily ration bacillus megaterium BM1259 preparation additive amount daily every be respectively as follows: 0g/d (control group), 5g/d, 10g/d and
15g/d studies influence of the bacillus megaterium BM1259 to milk cow in lactation period production performance, lumen fermentation and biochemical indicator.Knot
Fruit is as follows:
(1) milk yield, protein ratio and the cream of 10~15g bacillus megaterium BM1259 test group are added in daily ration
Rouge rate is significantly higher than control group.Neutral detergent fiber nutriment apparent digestibility 15g/d group is significantly higher than control group (table 1),
Wherein control group: bacillus megaterium BM1259 pitch-based sphere (0 gram/day), it is the same below.
Influence of the 1 bacillus megaterium BM1259 of table to milk cow in lactation period milk composition and digestibility of fiber
(2) there was no significant difference between cow rumen liquid pH value test group and control group, within the appropriate range；Between each group
Cud ammoniacal nitrogen, there was no significant difference for general volatile fatty acid concentration (table 2).
Influence of the 2 bacillus megaterium BM1259 of table to milk cow in lactation period Rumen Fermentation
(3) bacillus megaterium BM1259 microbial inoculum is added in daily ration, cow serum Histaglobin mean concentration is significantly high
In control group；There was no significant difference between gpt activity, glutamic-oxalacetic transaminease activity and uric acid each group；15g/d urea nitrogen is dense
Degree is significantly higher than control group (table 3).
Influence of the 3 bacillus megaterium BM1259 of table to milk cow in lactation period blood parameters
(4) excrement urea nitrogen concentration 15g/d group is substantially less than control group；Excrement ammonia nitrogen concentration 10g/d group be substantially less than pair
According to group, urine urea nitrogen concentration 15g/d group is substantially less than control group (table 4).
Influence of the 4 bacillus megaterium BM1259 of table to milk cow excreta nitrogen biochemical indicator
Cow-feeding is experiments have shown that milk cow production performance can be improved, promote cud by adding bacillus megaterium microbial inoculum in daily ration
Fermentation and reduce excreta in nitrogen loss.
Basic principles and main features and advantages of the present invention of the invention have been shown and described above.The skill of the industry
Art personnel it should be appreciated that the present invention is not limited to the above embodiments, the above embodiments and description only describe
The principle of the present invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these
Changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and
Its equivalent thereof.
Claims (8)
1. a kind of bacillus megaterium microbial inoculum, which is characterized in that it is by deposit number: the bacillus megaterium of GMCC No:1259 connects
Enter ingredient and proportion are as follows: sucrose 0.5%, cornstarch 0.8%, soybean cake powder 3%, corn starch 0.8%, peptone 0.5%, carbonic acid
The culture of calcium 0.05%, dipotassium hydrogen phosphate 0.08%, magnesium sulfate 0.13%, sodium chloride 0.1%, zinc sulfate 0.07%, potassium nitrate 0.01%
Base is made through liquid fermentation；Percentage is mass percent.
2. a kind of liquid fermentation process of bacillus megaterium microbial inoculum according to claim 1, which is characterized in that including such as
Lower step:
1) take deposit number: the bacillus megaterium of GMCC No:1259 is inoculated in thallus rejuvenation fluid nutrient medium, 30~34
DEG C, pH6~9,180rpm cultivate 36~40h, obtain the bacteria suspension that light absorption value OD is 1.0~1.2 under 600nm；
2) liquid fermentation medium is poured into fermentor sterilizing；The ingredient and proportion of liquid fermentation medium are as follows: sucrose 0.5%, jade
Rice starch 0.8%, soybean cake powder 3%, corn starch 0.8%, peptone 0.5%, calcium carbonate 0.05%, dipotassium hydrogen phosphate 0.08%, sulphur
Sour magnesium 0.13%, sodium chloride 0.1%, zinc sulfate 0.07%, potassium nitrate 0.01%；
3) by the bacterial suspension inoculation prepared by step 1) in liquid fermentation medium, bacterial suspension inoculation amount is liquid fermentation training
1.0~the 5.0%v/v for supporting matrix product, is passed through filtrated air, and 33 ± 1 DEG C, 40~46h of 180rpm fermented and cultured；In fermentation process,
It is sampled microscopy every 6 hours, observes bacterium form and bacillus exfoliation situation, when the bacillus exfoliation of 80% or more microscopy,
Terminate fermentation；
4) after fermentation terminates, fermentation liquid is centrifuged, collects thallus；The thallus of collection is placed in 45 DEG C of baking oven low temperature drying；It is dry
After crushed, bacillus megaterium BM1259 microbial inoculum is made.
3. liquid fermentation process according to claim 2, which is characterized in that manufactured bacillus megaterium in step 4)
The viable count of BM1259 microbial inoculum reaches 1010cfu/g。
4. liquid fermentation process according to claim 2, which is characterized in that the grinding screen used when being crushed in step 4)
Mesh number is 200 mesh.
5. a kind of purposes of the bacillus megaterium microbial inoculum described in claim 1 as additive for farm animal feed.
6. the liquid fermentation process of bacillus megaterium microbial inoculum described in claim 2-4 any one adds preparing animal and fowl fodder
Add the purposes in agent.
7. a kind of purposes of the bacillus megaterium microbial inoculum described in claim 1 as cow feed additive.
8. milk cow in lactation period daily ration, which is characterized in that by bacillus megaterium microbial inoculum described in claim 1 and milk cow basis
Daily ration composition, milk cow basal diet group become corn silage 43%, corn 15.83%, soybean skin 5.8%, corn tabletting 5.72%,
DDGS5.68%, beet pulp 4%, clover 4%, dregs of beans 3.52%, cottonseed 3%, Cottonseed Meal 2.86%, corn germ cake 2.5%, brewex's grains
2.46%, calcium monohydrogen phosphate 0.44%, calcium carbonate 0.35%, sodium bicarbonate 0.33%, salt 0.22%, premix 0.18% and yeast culture
0.11%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910567038.XA CN110317753A (en) | 2019-06-27 | 2019-06-27 | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910567038.XA CN110317753A (en) | 2019-06-27 | 2019-06-27 | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110317753A true CN110317753A (en) | 2019-10-11 |
Family
ID=68121247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910567038.XA Pending CN110317753A (en) | 2019-06-27 | 2019-06-27 | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110317753A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN113913324A (en) * | 2021-09-01 | 2022-01-11 | 郑州轻工业大学 | Composite microbial inoculum for efficient deodorization of biological filter and preparation method thereof |
| CN114702357A (en) * | 2022-01-13 | 2022-07-05 | 山东绿邦生物科技有限公司 | High-activity composite microbial fertilizer and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786151A (en) * | 2005-08-15 | 2006-06-14 | 戴承镛 | Large bacillus, its preparation method and use |
| CN101999519A (en) * | 2010-11-02 | 2011-04-06 | 戴承镛 | Deodorization conditioner and preparation method thereof for bacillus megaterium |
| CN104480052A (en) * | 2015-01-05 | 2015-04-01 | 江苏省苏微微生物研究有限公司 | Composite bacillus preparation containing three strains, preparation method of composite bacillus preparation and application of composite bacillus preparation to ecological breeding |
| CN106010995A (en) * | 2016-01-07 | 2016-10-12 | 扬州大学 | Fermentation method of Bacillus megaterium preparation |
| RU2652832C1 (en) * | 2017-08-02 | 2018-05-03 | Общество с ограниченной ответственностью "БИОТРОФ" (ООО "БИОТРОФ") | Method of feeding farm birds |
-
2019
- 2019-06-27 CN CN201910567038.XA patent/CN110317753A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786151A (en) * | 2005-08-15 | 2006-06-14 | 戴承镛 | Large bacillus, its preparation method and use |
| CN101999519A (en) * | 2010-11-02 | 2011-04-06 | 戴承镛 | Deodorization conditioner and preparation method thereof for bacillus megaterium |
| CN104480052A (en) * | 2015-01-05 | 2015-04-01 | 江苏省苏微微生物研究有限公司 | Composite bacillus preparation containing three strains, preparation method of composite bacillus preparation and application of composite bacillus preparation to ecological breeding |
| CN106010995A (en) * | 2016-01-07 | 2016-10-12 | 扬州大学 | Fermentation method of Bacillus megaterium preparation |
| RU2652832C1 (en) * | 2017-08-02 | 2018-05-03 | Общество с ограниченной ответственностью "БИОТРОФ" (ООО "БИОТРОФ") | Method of feeding farm birds |
Non-Patent Citations (2)
| Title |
|---|
| 余同水: "巨大芽孢杆菌对泌乳期奶牛生产性能、瘤胃发酵及生化指标的影响", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 王锋 等: "《高产奶牛养殖7日通》", 31 January 2004, 中国农业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN111925964B (en) * | 2020-08-21 | 2022-04-22 | 扬州大学 | Bacillus megaterium and application thereof |
| CN113913324A (en) * | 2021-09-01 | 2022-01-11 | 郑州轻工业大学 | Composite microbial inoculum for efficient deodorization of biological filter and preparation method thereof |
| CN113913324B (en) * | 2021-09-01 | 2023-06-30 | 郑州轻工业大学 | Composite microbial inoculant for efficient deodorization of biological filter and preparation method thereof |
| CN114702357A (en) * | 2022-01-13 | 2022-07-05 | 山东绿邦生物科技有限公司 | High-activity composite microbial fertilizer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102181376B (en) | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof | |
| CN101463329B (en) | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof | |
| CN103275907B (en) | Bacillus amyloliquefacien and preparation method and application thereof | |
| CN101914460B (en) | Method for fermenting phodotorula benthica culture | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN107801837B (en) | Biological fermentation process for forage-based utilization of chicken manure | |
| CN111838408A (en) | Functional amino acid enzyme and preparation method thereof | |
| CN107312732B (en) | Probiotic feed additive | |
| CN103087964A (en) | Bacillus subtilis, microecological preparation and application of bacillus subtilis in animal feed | |
| CN113604404B (en) | Bacillus coagulans YSF17 and application thereof | |
| CN110317753A (en) | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application | |
| CN104012803A (en) | Preparation method of fermented feed capable of preventing cow mastitis | |
| CN110218688A (en) | Hai Neishi bacillus LY-23, microbial inoculum and its application and the product for applying it | |
| CN102406068B (en) | Method for preparing microbial fermented feed capable of keeping intestinal health of pigs | |
| CN115067419A (en) | Application of feed additive and preparation method of earthworm fermentation liquor | |
| CN106071143B (en) | A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved | |
| CN102559560A (en) | Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis | |
| CN106035988B (en) | Production method of arginine active peptide powder for livestock and poultry | |
| CN107760612A (en) | A kind of aspergillus niger yy07 bacterial strains and its application in solid fermentation produces feeding acid protease | |
| CN106819625A (en) | A kind of mandarin fish fermented feed and preparation method thereof | |
| CN116478882A (en) | Preparation method of acid-producing mixed bacteria preparation capable of improving sheep daily gain and feed conversion rate | |
| CN107198038B (en) | Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs | |
| CN109170149A (en) | A method of microecological fermented forage is produced using antibiotic milk | |
| CN108441452A (en) | A kind of store pig compound microbial culture starter and its application | |
| CN109321478A (en) | It is a kind of degrade mycotoxin bacterial strain yk18 and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191011 |
|
| RJ01 | Rejection of invention patent application after publication |