CN102406068B - Method for preparing microbial fermented feed capable of keeping intestinal health of pigs - Google Patents
Method for preparing microbial fermented feed capable of keeping intestinal health of pigs Download PDFInfo
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- CN102406068B CN102406068B CN2011103165252A CN201110316525A CN102406068B CN 102406068 B CN102406068 B CN 102406068B CN 2011103165252 A CN2011103165252 A CN 2011103165252A CN 201110316525 A CN201110316525 A CN 201110316525A CN 102406068 B CN102406068 B CN 102406068B
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Abstract
The invention provides a method for preparing microbial fermented feed capable of keeping intestinal health of pigs, comprising the following steps: activating the strains of lactobacillus plantarum and the strains of clostridium butyricum; fermenting and cultivating the first liquid, fermenting and cultivating the second liquid, and fermenting and cultivating the third liquid; freezing and drying the solid fermentation culture of the lactobacillus plantarum and the solid fermentation culture of the clostridium butyricum, and mixing to obtain the microbial fermented feed. The invention has good biological function, keeps the micro-ecological balance of the intestinal tract, improves the production volume, saves the drug cost, and greatly improves the pig raising efficiency. The preparation method of the invention is simple and practical, and has large function and good market prospect.
Description
Technical field
The present invention relates to a kind of preparation method that can protect the fermentative feedstuff of microbe of chitling road health, it is healthy to be mainly used in improving the chitling road, belongs to the probiotics technical field.
Background technology
Along with improving constantly of people's living standard, the quality requirement of livestock products improves constantly, the scale of aquaculture forward, standardization future development.In pig industry, the resistance against diseases of pig, digestion power are very crucial.At present, the interpolation of antibiotic and hormone is very general in the pig feed, the feed micro-ecological additive is unstable to high temperature, hydrochloric acid in gastric juice and cholate, cause and have activity after only having the minority bacterial strain to enter enteron aisle, thereby do not reach the needed number of viable that plays a role, also have in addition some these unstable, that the shelf-life is short problems, thereby affected the growth result of pig.Correct raising pig and science to implement method most important, allow be grown normally, grow in the pig body, prevent indigestion and insufficient to absorption of nutrient ingredients.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method that can protect the fermentative feedstuff of microbe of chitling road health; the fermentative feedstuff of microbe of gained has effective chitling road environment that improves; promote digestion; improve the speed of growth of pig; improve the effect of resistance against diseases, the development of pig industry is had great significance.
In order to achieve the above object, the invention provides a kind of preparation method of fermentative feedstuff of microbe, it is characterized in that, comprise: with Lactobacillus plantarum bacterial classification and clostridium butyricum bacterial classification through overactivation, for the first time liquid fermentation and culture, after liquid fermentation and culture and solid fermentation are cultivated for the second time, with Lactobacillus plantarum solid fermentation culture and the freeze drying of clostridium butyricum solid fermentation culture of gained, and be mixed to get fermentative feedstuff of microbe.
Preferably, the activation step of described Lactobacillus plantarum bacterial classification is: be in 0.5%～1.5% the physiological saline with the weight percent concentration of Lactobacillus plantarum bacterial classification inoculation after the sterilization, concussion makes its abundant mixing, leaves standstill activation.
Preferably, the activation step of described clostridium butyricum bacterial classification is: be in 0.5%～1.5% the physiological saline with the weight percent concentration of clostridium butyricum bacterial classification inoculation after the sterilization, concussion makes its abundant mixing, leaves standstill activation.
Preferably, described Lactobacillus plantarum bacterial classification the first time liquid fermentation and culture step be: in brewer's wort, add yeast extract and calcium carbonate and obtain liquid fermentation medium, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1～1:0.1～1, liquid fermentation medium is sterilized, Lactobacillus plantarum bacterial classification inoculation after the activation in described liquid fermentation medium, is carried out liquid fermentation and culture at shaking table.
Preferably, described clostridium butyricum bacterial classification the first time liquid fermentation and culture step be: in distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5～4:0.5～3:0.1～1, liquid fermentation medium is sterilized, clostridium butyricum bacterial classification inoculation after the activation in described liquid fermentation medium, is carried out liquid fermentation and culture at shaking table.
Preferably, described Lactobacillus plantarum bacterial classification the second time liquid fermentation and culture step be: in brewer's wort, add yeast extract and calcium carbonate and obtain liquid fermentation medium, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1～1:0.1～1, liquid fermentation medium is sterilized, the product of liquid fermentation and culture is inoculated in the described liquid fermentation medium with the first time, carries out liquid fermentation and culture at shaking table.
Preferably, described clostridium butyricum bacterial classification the second time liquid fermentation and culture step be: in distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5～4:0.5～3:0.1～1, liquid fermentation medium is sterilized, the product of liquid fermentation and culture is inoculated in the described liquid fermentation medium with the first time, carries out liquid fermentation and culture at shaking table.
Preferably, the step that the solid fermentation of described Lactobacillus plantarum bacterial classification is cultivated is: corn flour, wheat bran and dregs of beans are mixed to get solid mixture, the weight ratio of corn flour, wheat bran and dregs of beans is 50～60:20～25:15～20, solid mixture is mixed by weight 100:10～30 with skimmed milk power, after the sterilization, inoculation is the product of liquid fermentation and culture for the second time, mix, the mixture of gained is shakeout, thickness remains on 15～20cm, cover film and seal, under 30～40 ℃, carry out solid fermentation and cultivate.
Preferably, the step that the solid fermentation of described clostridium butyricum bacterial classification is cultivated is: with corn flour, molasses, soybean-cake flour and calcium carbonate are mixed to get solid mixture, corn flour, molasses, the weight ratio of soybean-cake flour and calcium carbonate is 30～40:30～40:10～15:5～10, solid mixture is mixed by weight 100:10～30 with skimmed milk power, after the sterilization, inoculation is the product of liquid fermentation and culture for the second time, mix, the mixture of gained is shakeout, thickness remains on 15～20cm, cover film and seal, under 30～40 ℃, carry out solid fermentation and cultivate.
Preferably, after the described freeze drying, the moisture weight content of Lactobacillus plantarum solid fermentation culture and clostridium butyricum solid fermentation culture all is lower than 10%.
Preferably, the mixed weight ratio of described Lactobacillus plantarum solid fermentation culture and clostridium butyricum solid fermentation culture is 10～18:12～15.
The present invention has good biological function, can keep the intestinal microecology balanced action, improve gut flora, antimutagenic effect, the speed of growth to pig improves a lot, effectively reduce the diarrhea rate of pig and the death rate of young pig, improved production capacity and saved drug cost, greatly improved the efficient of raising pigs.Preparation method of the present invention is simple and practical, and is powerful, has good market prospects.
The specific embodiment
Specify the present invention below in conjunction with embodiment.
Embodiment 1: the preparation method of fermentative feedstuff of microbe
(1) activation: with Lactobacillus plantarum bacterial classification (Lactobacillus planta, available from Chinese agriculture microbial preservation administrative center, deposit number ACCC11118) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 0.9% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.With clostridium butyricum bacterial classification (Clostridium butyricum, available from Chinese microorganism strain preservation center, deposit number CGMCC 1.0209) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 0.9% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.
(2) preparation of first order seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1:0.1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, Lactobacillus plantarum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5:0.5:0.1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, clostridium butyricum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
(3) preparation of secondary seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1:0.1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the Lactobacillus plantarum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5:0.5:0.1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the clostridium butyricum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
(4) solid fermentation is cultivated: corn flour, wheat bran and dregs of beans are mixed to get solid mixture, the weight ratio of corn flour, wheat bran and dregs of beans is 50:25:20, solid mixture is mixed by weight 100:10 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, according to volume ratio 100:5 inoculation Lactobacillus plantarum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 15cm, cover film and seal, under 30 ℃, cultivate 48h.
Corn flour, molasses, soybean-cake flour and calcium carbonate are mixed to get solid mixture, the weight ratio of corn flour, molasses, soybean-cake flour and calcium carbonate is 30:40:15:10, solid mixture is mixed by weight 100:10 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, with volume ratio 100:10 inoculation clostridium butyricum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 15cm, cover film and seal, under 30 ℃, cultivate 48h.
(5) Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are respectively in freeze drier the inside freeze-drying, until the moisture weight content in the solid mixt all is lower than 10%, can stop drying, after crushed, Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are mixed by weight 1.5:2, obtain fermentative feedstuff of microbe.
Embodiment 2: the preparation method of fermentative feedstuff of microbe
(1) activation: with Lactobacillus plantarum bacterial classification (Lactobacillus planta, available from Chinese agriculture microbial preservation administrative center, deposit number ACCC11118) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 1.5% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.With clostridium butyricum bacterial classification (Clostridium butyricum, available from Chinese microorganism strain preservation center, deposit number CGMCC 1.0209) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 1.5% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.
(2) preparation of first order seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:1:1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, Lactobacillus plantarum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:4:3:1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, clostridium butyricum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
(3) preparation of secondary seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:1:1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the Lactobacillus plantarum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:4:3:1, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the clostridium butyricum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
(4) solid fermentation is cultivated: corn flour, wheat bran and dregs of beans are mixed to get solid mixture, the weight ratio of corn flour, wheat bran and dregs of beans is 60:20:15, solid mixture is mixed by weight 100:30 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, according to volume ratio 100:5 inoculation Lactobacillus plantarum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 20cm, cover film and seal, under 40 ℃, cultivate 48h.
Corn flour, molasses, soybean-cake flour and calcium carbonate are mixed to get solid mixture, the weight ratio of corn flour, molasses, soybean-cake flour and calcium carbonate is 40:30:10:5, solid mixture is mixed by weight 100:30 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, with volume ratio 100:10 inoculation clostridium butyricum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 20cm, cover film and seal, under 40 ℃, cultivate 48h.
(5) Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are respectively in freeze drier the inside freeze-drying, until the moisture weight content in the solid mixt all is lower than 10%, can stop drying, after crushed, Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are mixed by weight 1.8:1.5, obtain fermentative feedstuff of microbe.
Embodiment 3: the preparation method of fermentative feedstuff of microbe
(1) activation: with Lactobacillus plantarum bacterial classification (Lactobacillus planta, available from Chinese agriculture microbial preservation administrative center, deposit number ACCC11118) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 0.9% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.With clostridium butyricum bacterial classification (Clostridium butyricum, available from Chinese microorganism strain preservation center, deposit number CGMCC 1.0209) is inoculated into through 121 ℃ of sterilizations 20 minutes and is cooled to 25 ℃ weight percent concentration in 0.9% physiological saline 10ml take volume ratio 2:100, concussion makes its abundant mixing, leaves standstill activation 30 minutes.
(2) preparation of first order seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.5:0.6, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, Lactobacillus plantarum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:2:1.5:0.2, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, clostridium butyricum bacterial classification after the activation is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as first order seed on shaking table.
(3) preparation of secondary seed: adding yeast extract and calcium carbonate obtain liquid fermentation medium in brewer's wort, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.5:0.6, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the Lactobacillus plantarum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
In distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:2:1.5:0.2, liquid fermentation medium 100ml is placed in the 250ml triangular flask, through 121 ℃ of sterilizations 20 minutes and be cooled to 25 ℃, the clostridium butyricum first order seed is inoculated in the described liquid fermentation medium with volume ratio 1:10, with 37 ℃, 180 rev/mins of anaerobism of shaking speed are cultivated 24 hours as secondary seed on shaking table.
(4) solid fermentation is cultivated: corn flour, wheat bran and dregs of beans are mixed to get solid mixture, the weight ratio of corn flour, wheat bran and dregs of beans is 55:25:17, solid mixture is mixed by weight 100:20 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, according to volume ratio 100:5 inoculation Lactobacillus plantarum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 17cm, cover film and seal, under 35 ℃, cultivate 48h.
Corn flour, molasses, soybean-cake flour and calcium carbonate are mixed to get solid mixture, the weight ratio of corn flour, molasses, soybean-cake flour and calcium carbonate is 35:35:12:7, solid mixture is mixed by weight 100:20 with skimmed milk power, under 100 ℃ of temperature, steamed 40 minutes, then be cooled to 25 ℃, with volume ratio 100:10 inoculation clostridium butyricum secondary seed, mix, the mixture of gained is shakeout, thickness remains on 17cm, cover film and seal, under 35 ℃, cultivate 48h.
(5) Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are respectively in freeze drier the inside freeze-drying, until the moisture weight content in the solid mixt all is lower than 10%, can stop drying, after crushed, Lactobacillus plantarum solid fermentation product and clostridium butyricum solid fermentation product are mixed by weight 2:1.5, obtain fermentative feedstuff of microbe.
Application examples
1, material: the fermentative feedstuff of microbe of gained of the present invention.
2, experimental animal and grouping: select 40 of 90 age in days weanling pigs, be divided into control group and test group, 20 every group.
3, test method:
Control group: the basal diet+normal diet of feeding, weight ratio are 9:1.
Test group: the fermentative feedstuff of microbe of the basal diet of feeding+gained of the present invention, weight ratio are 9:1.
Take group as unit, the data such as the every nest piglet of detail record every morning weight, diarrhoea.Calculate at last the performance indications such as average daily gain, average cost, diarrhoea ratio situation of full phase, contrast as follows:
| Group | Number | Diarrhoea ratio % | Sick ratio % | Head is medical expense all | Average daily gain | The pig house smell |
| Control group | 20 | 10% | 20% | 13.50 unit | 364g | Stink is heavy |
| Test group | 20 | 0% | 5% | 6.10 unit | 545g | Stink is light |
Interpretation of result: product of the present invention effectively reduces diarrhea rate and the sick rate of growing and fattening pigs, and medical expense has reduced more than 50%, Effective Raise adding weight, alleviated the stink of discharging ight soil, otherness is remarkable.Comprehensively improve the speed of growth of pig, improved production capacity and saved drug cost, greatly improved the efficient of raising pigs.
Claims (10)
1. the preparation method of a fermentative feedstuff of microbe is characterized in that, comprising: with deposit number be ACCC11118 the Lactobacillus plantarum bacterial classification (
Lactobacillus planta) and deposit number be CGMCC 1.0209 the clostridium butyricum bacterial classification (
Clostridium butyricum) through overactivation, for the first time liquid fermentation and culture, after liquid fermentation and culture and solid fermentation are cultivated for the second time, with Lactobacillus plantarum solid fermentation culture and the freeze drying of clostridium butyricum solid fermentation culture of gained, and be mixed to get fermentative feedstuff of microbe.
2. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, the activation step of described Lactobacillus plantarum bacterial classification is: be in 0.5%～1.5% the physiological saline with the weight percent concentration of Lactobacillus plantarum bacterial classification inoculation after the sterilization, concussion makes its abundant mixing, leaves standstill activation.
3. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, the activation step of described clostridium butyricum bacterial classification is: be in 0.5%～1.5% the physiological saline with the weight percent concentration of clostridium butyricum bacterial classification inoculation after the sterilization, concussion makes its abundant mixing, leaves standstill activation.
4. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, described Lactobacillus plantarum bacterial classification the first time liquid fermentation and culture step be: in brewer's wort, add yeast extract and calcium carbonate and obtain liquid fermentation medium, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1～1:0.1～1, liquid fermentation medium is sterilized, Lactobacillus plantarum bacterial classification inoculation after the activation in described liquid fermentation medium, is carried out liquid fermentation and culture at shaking table.
5. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, described clostridium butyricum bacterial classification the first time liquid fermentation and culture step be: in distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5～4:0.5～3:0.1～1, liquid fermentation medium is sterilized, clostridium butyricum bacterial classification inoculation after the activation in described liquid fermentation medium, is carried out liquid fermentation and culture at shaking table.
6. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, described Lactobacillus plantarum bacterial classification the second time liquid fermentation and culture step be: in brewer's wort, add yeast extract and calcium carbonate and obtain liquid fermentation medium, the weight ratio of brewer's wort, yeast extract and calcium carbonate is 100:0.1～1:0.1～1, liquid fermentation medium is sterilized, the product of liquid fermentation and culture is inoculated in the described liquid fermentation medium with the first time, carries out liquid fermentation and culture at shaking table.
7. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, described clostridium butyricum bacterial classification the second time liquid fermentation and culture step be: in distilled water, add molasses, cornstarch and calcium carbonate and obtain liquid fermentation medium, the weight ratio of distilled water, molasses, cornstarch and calcium carbonate is 100:0.5～4:0.5～3:0.1～1, liquid fermentation medium is sterilized, the product of liquid fermentation and culture is inoculated in the described liquid fermentation medium with the first time, carries out liquid fermentation and culture at shaking table.
8. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, the step that the solid fermentation of described Lactobacillus plantarum bacterial classification is cultivated is: with corn flour, wheat bran and dregs of beans are mixed to get solid mixture, corn flour, the weight ratio of wheat bran and dregs of beans is 50～60:20～25:15～20, solid mixture is mixed by weight 100:10～30 with skimmed milk power, after the sterilization, inoculation is the product of liquid fermentation and culture for the second time, mix, the mixture of gained is shakeout, thickness remains on 15～20cm, covers film and seals, and carries out solid fermentation and cultivate under 30～40 ℃.
9. the preparation method of fermentative feedstuff of microbe as claimed in claim 1, it is characterized in that, the step that the solid fermentation of described clostridium butyricum bacterial classification is cultivated is: with corn flour, molasses, soybean-cake flour and calcium carbonate are mixed to get solid mixture, corn flour, molasses, the weight ratio of soybean-cake flour and calcium carbonate is 30～40:30～40:10～15:5～10, solid mixture is mixed by weight 100:10～30 with skimmed milk power, after the sterilization, inoculation is the product of liquid fermentation and culture for the second time, mix, the mixture of gained is shakeout, thickness remains on 15～20cm, covers film and seals, and carries out solid fermentation and cultivate under 30～40 ℃.
10. the preparation method of fermentative feedstuff of microbe as claimed in claim 1 is characterized in that, the mixed weight ratio of described Lactobacillus plantarum solid fermentation culture and clostridium butyricum solid fermentation culture is 10～18:12～15.
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| CN103283943B (en) * | 2013-05-31 | 2015-04-15 | 青岛根源生物技术集团有限公司 | Compound microbiological preparation for fermenting soybean meal and applications of preparation |
| CN103392923A (en) * | 2013-07-23 | 2013-11-20 | 上海创博生态工程有限公司 | Microorganism feed additive for improving reproduction ability of animals and preparation method thereof |
| CN103609870B (en) * | 2013-10-08 | 2015-10-21 | 天津市畜牧兽医研究所 | Take DDGS as the full fermented feed preparation method of large pig of major protein raw material |
| CN103875975A (en) * | 2014-03-19 | 2014-06-25 | 淮北嘉吉农牧科技有限公司 | Pig feed for adjusting intestines and stomach and preparation method of feed |
| CN104186923A (en) * | 2014-07-14 | 2014-12-10 | 陈方龙 | Method for producing efficient feed by treating non-conventional feed raw material resources through multilevel fermentation |
| CN104920915A (en) * | 2015-07-15 | 2015-09-23 | 紫金县蓝野农业开发有限公司 | Microbial fermentation pig feed |
| CN105166385A (en) * | 2015-09-23 | 2015-12-23 | 安徽省兴源草业有限公司 | Fermented feed with ragimillet seeds and fermenting agent |
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| CN1663573A (en) * | 2004-03-04 | 2005-09-07 | 青岛东海药业有限公司 | A stable and safe microecological formulation, its preparation and usage |
| CN1957953A (en) * | 2005-10-14 | 2007-05-09 | 德阳创新生物工程有限公司 | Composition of multiple fungus possessing immunoloregulation function, preparation method and usage |
| CN101485402A (en) * | 2009-02-16 | 2009-07-22 | 北京市农林科学院 | Composite biological feed additive agent for fattening early weaning mutton sheep |
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