CN102907572A - Banana silage microbial additive and preparation method thereof - Google Patents
Banana silage microbial additive and preparation method thereof Download PDFInfo
- Publication number
- CN102907572A CN102907572A CN2012104037519A CN201210403751A CN102907572A CN 102907572 A CN102907572 A CN 102907572A CN 2012104037519 A CN2012104037519 A CN 2012104037519A CN 201210403751 A CN201210403751 A CN 201210403751A CN 102907572 A CN102907572 A CN 102907572A
- Authority
- CN
- China
- Prior art keywords
- banana
- plant lactobacillus
- additive
- stem leaves
- ensiling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to a banana silage microbial additive and a preparation method thereof. The microbial additive consists of trichoderma viride, candida utilis, plant lactobacillus, banana stem and leaf powder and the like, and is prepared by a method of firstly performing liquid culture and then performing solid culture. The microbial additive is applied to silage of banana stems and leaves; the fermentation time for preparing the silage is short; after the silage is fermented, the coarse fiber content of the banana stems and leaves is reduced by 20 percent, the crude protein is increased by over 30 percent, the prepared feed has rich sour aroma, the utilization rate of the banana stems and leaves is increased and improved, and the research level reaches the national advanced level.
Description
Technical field
The present invention relates to a kind of microbe additive, a kind of additive and application for preparing feed for the banana stem leaves silage fermentation belongs to the ensilage fermentation technical field specifically.
Background technology
Ensilage is a kind of high-quality succulent fodder that fresh crop forms through the anaerobe fermentation, and its nutriment is preserved, and the activity of microorganism can produce the required little molecular nutrition material of a lot of animals during the fermentation.Microbial cells itself can provide mycoprotein, adds the cellulose components that certain microorganism can degrade in the raw material and is converted into micromolecular monose, oligosaccharides or compound sugar.Beneficial microorganism is movable in the ensilage, and the lactic acid that produces such as lactic acid bacteria can kill the disease microorganism, parasitic ovum of a lot of livestocks etc., improves the flora of beneficial bacterium in the livestock body, keeps the balance of beneficial bacterium, thereby improves the immunity of organisms of livestock.Artificial some ensiling agent of interpolation promote beneficial microorganisms movable in the ensiling raw material, can play preventing disease theraping effect.The microbionation agent is compared with chemical addition agent, have safe, easy to operate, without advantages such as burn into are free from environmental pollution, therefore be widely adopted in recent years.
Summary of the invention
The object of the invention is to provides a kind of microoganism additives for ensiling for weak points such as banana stem leaves are high as the tannin content of feed own, crude fibre is many, crude protein content is few.The present invention makes microbe additive by adding the banana stem leaves powder carrier after the major function bacterium is cultivated, and the additive of this method preparation has very strong adaptability in the banana stem leaves fermentation.
Technical scheme of the present invention is:
A kind of banana microbial additive for silage fodder is characterized in that: described microbe additive is comprised of Trichoderma viride, candida utili and plant lactobacillus and carrier.
Described plant lactobacillus separates from sauerkraut, screen and obtain.
Described Trichoderma viride: candida utili: the volume ratio of plant lactobacillus is 1:2:1.
The carrier of described microbe additive is the solid medium of banana stem leaves powder and the preparation of auxiliary material cane molasses.
The preparation method of described microbe additive: described plant lactobacillus dilution spread and line partition method by routine from banana garden soil, commercially available sauerkraut and other feed addictives filters out the plant lactobacillus that is suitable for the banana stem leaves ensiling; Plant lactobacillus, Trichoderma viride and the candida utili of screening are cultivated with MRS, PDA and brewer's wort culture medium slant respectively first, enlarged with fluid nutrient medium respectively again and cultivate; Be linked into after by volume ratio 1:2:1 mixes with plant lactobacillus, Trichoderma viride and candida utili nutrient solution after enlarge cultivating that 27 ℃ ~ 30 ℃ airtight cultivations were prepared into solid additive in 3 ~ 5 days in the solid medium of banana stem leaves powder and the preparation of auxiliary material cane molasses.
Microbe additive is solid, need be watered rear use when being used for the banana stem leaves ensiling, and the pH value of ensilage was between 4 ~ 5 after ensiling was finished.
Use microbe additive of the present invention and prepare the banana stem leaves ensilage, the fermentation time of preparation ensilage is short, can significantly improve its nutritive value, its mycoprotein and microbe metabolite obviously increase, the banana stem leaves crude fiber content reduces by 20% behind the silage fermentation, crude protein improves more than 35%, and the feed aromatic flavour of making has improved and improved the utilization rate of banana stem leaves.
The specific embodiment
Below in conjunction with specific embodiment, the present invention will be described, and the experimental technique among the embodiment and used reagent and equipment are conventional method, conventional reagent and equipment unless otherwise noted.
Embodiment 1
One, bacterial classification
Trichoderma viride strain: be purchased from microorganism fungus kind preservation center, Guangdong Microbes Inst, deposit number: GIM3.141;
Candida utili bacterial strain: be purchased from microorganism fungus kind preservation center, Guangdong Microbes Inst, deposit number: GIM2.148;
Plant lactobacillus: from banana garden soil, commercially available sauerkraut and other feed addictives, filter out the plant lactobacillus that is suitable for the banana stem leaves ensiling.
Two, culture medium
The Trichoderma viride culture medium: potato juice (200g peeling potato is cut into small pieces, and cold water is put into behind the boiling 20min with getting juice after 4 layers of filtered through gauze) adds 20g glucose, adds 5% agar when being used for the inclined-plane cultivation; Seed culture medium does not add agar.
The candida utili culture medium: the raw material (not hopping) with fermentation beer, be diluted to 10 Berlin, be used for adding when the inclined-plane is cultivated 2% agar; Seed culture medium does not add agar.
Plant lactobacillus culture medium: casein peptone 10.0g/L, beef leaching thing 10.0g/L, yeast extract 4.0g/L, glucose 10.0g/L, Tween 80 1.0mL/L, dipotassium hydrogen phosphate 2.0g/L, sodium acetate 5.0g/L, dibasic ammonium citrate 2.0g/L, epsom salt 0.2g/L, seven water manganese sulfate 0.05g/L are used for adding when the inclined-plane is cultivated 5% agar; Seed culture medium does not add agar.
Three, the preparation method of bacterial classification
The cultivation of Trichoderma viride bacterial classification: get the Trichoderma viride freeze-drying Guan Yizhi of purchase, in superclean bench, bacterial classification activated at slant medium, need continuous two generation renewal cultivation; Get eugonic Trichoderma viride and in fluid nutrient medium, enlarge cultivation with the spore inoculating that it is good that the transfer needle picking is grown, cultivated 5 days at 28 ℃.The cultivation of candida utili bacterial classification: get the candida utili freeze-drying Guan Yizhi of purchase, in superclean bench, bacterial classification activated at slant medium, need continuous two generation renewal cultivation; Get eugonic candida utili and be inoculated in the thalline that it is good that the transfer needle picking is grown and enlarge cultivation in the fluid nutrient medium, cultivated 2 days at 28 ℃.
The cultivation of plant lactobacillus bacterial classification: will screen the good plant lactobacillus that is applicable to the banana stem leaves ensiling and cultivate vigorous bacterial classification at the MRS solid medium, be inoculated in the thalline that it is good that the transfer needle picking is grown and enlarge cultivation in the fluid nutrient medium, cultivated 3 days at 37 ℃.
Four, the preparation method of microbe additive
A kind of banana stem leaves microoganism additives for ensiling is prepared from by the addition fermentation according to 2 ~ 6:100 of microorganism mixed-culture medium and solid medium; The optimum addition of described microorganism mixed-culture medium is 4:100; Described solid medium is put into the retort pouch high-temperature sterilization by banana stem leaves powder and molasses according to the ratio mixing of banana stem leaves powder: molasses=8:2 and is made; Described microorganism mixed-culture medium comprises that Trichoderma viride, candida utili and plant lactobacillus nutrient solution mix by a certain percentage; Described plant lactobacillus dilution spread and line partition method by routine from banana garden soil, commercially available sauerkraut and other feed addictives filters out the plant lactobacillus that is suitable for the banana stem leaves ensiling; The mixed volume of described 3 bacterium is than being Trichoderma viride: candida utili: plant lactobacillus=1:2:1, prepare three kinds of seed liquor, the various seed culture fluids that prepare are linked in proportion in the solid medium of banana stem leaves powder and the preparation of auxiliary material cane molasses, keep 27 ℃ ~ 30 ℃ of temperature, fermenting in the closed container was prepared into solid additive in 3 ~ 5 days.These microbe additive number of viable content 〉=10
6Individual/gram, the pH value of ensilage is between 4 ~ 5 after ensiling finishes.
Embodiment 2
The present embodiment provides the using method of the microbe additive of banana stem leaves ensiling: the microbial solid additive for preparing is added 2kg normal temperature fresh water according to the 1kg additive, make resurgent water after fully stirring, contain the viable bacteria amount in the resurgent water 10
6Cfu/mL ~ 10
8Cfu/mL.Banana stem leaves is cut into two groups of 1cm ~ 2cm and 4cm ~ 5 cm, process to water content about 60% ~ 65%, the resurgent water made 2% ~ 4% is stirred with banana stem leaves by volume, in the fermenter of the 50L that packs into, compacting, airtight after at normal temperatures lucifuge cultivated 25 ~ 30 days.The quality of ensilage sees Table 1.
Table 1 ensilage on-site evaluation score
Compare with the ensiling raw material, ensilage can be found out according to result of the test after processing: what material was cut is little all better than large its color, smell and quality.Material is cut to such an extent that press greatly unreally, and other varied bacteria growings are preponderated, and material pH value and moisture raise, cause material in ensilage, to occur corrupt, rotten, the color blackening, the rear material of ensiling end becomes sticky not soft, carries out ensiling so select that banana stem leaves is cut into 1cm ~ 2cm.
Embodiment 3
The present embodiment provides the using method of the microbe additive of banana stem leaves ensiling: the microbial solid additive for preparing is added 2kg normal temperature fresh water according to the 1kg additive, make resurgent water after fully stirring, contain the viable bacteria amount in the resurgent water 10
6Cfu/mL ~ 10
8Cfu/mL.Banana stem leaves is cut into 1cm ~ 2cm, process to water content 60% ~ 65% and 70% ~ 75% liang of group, the resurgent water made 2% ~ 4% is stirred with banana stem leaves by volume, in the fermenter of the 50L that packs into, compacting, airtight after at normal temperatures lucifuge cultivated 25 ~ 30 days.The quality of ensilage sees Table 2.
Table 2 ensilage on-site evaluation score
Compare with the ensiling raw material, ensilage can be found out according to result of the test after processing: moisture content of material is little few all better than its color, smell and the quality of Duoing.Many other varied bacteria growings of material moisture are preponderated, material pH value and moisture raise, and cause material to occur in ensilage corrupt, mildew and rot, the color blackening, the material caking that is clamminess after ensiling finishes is so select the processing of banana stem leaves water content is carried out ensiling to about 60% ~ 65%.
Embodiment 4
The present embodiment provides the using method of the microbe additive of banana stem leaves ensiling: the microbial solid additive for preparing is added 2kg normal temperature fresh water according to the 1kg additive, make resurgent water after fully stirring, contain the viable bacteria amount in the resurgent water 10
6Cfu/mL ~ 10
8Cfu/mL.Banana stem leaves is cut into 1cm ~ 2cm, process to water content about 60% ~ 65%, the resurgent water made 2% ~ 4% is stirred with banana stem leaves by volume, in the fermenter of the 50L that packs into, compacting, airtight after at normal temperatures lucifuge cultivate and got final product in 25 ~ 30 days.The Variation of Main Nutritional Components situation sees Table 3, and the quality of ensilage sees Table 4.
Table 3 Variation of Main Nutritional Components situation
| Testing index | Before the processing | After the processing |
| The pH value | 5.27 | 3.96 |
| Dry (%) | 38.39 | 33.45 |
| Crude protein (%) | 4.42 | 6.13 |
| Ammonium nitrogen (mg/L) | 0.44 | 0.61 |
| Crude fibre (%) | 46.8 | 36.6 |
| Soluble-carbohydrate (%) | 6.11 | 3.05 |
Table 4 ensilage on-site evaluation score
Compare before and after processing, banana stem leaves adds the microoganism additives for ensiling ensiling finish after the pH of ensilage below 4, dry reduces before than ensiling to some extent; Crude protein content increases to some extent, and the content recruitment reaches 37%; Ammonium nitrogen increases, but total content is still very low; Crude fiber content decreases before than ensiling, and its content reduction reaches 22%; Carry out the scene scoring according to the evaluation criteria of ensilage quality behind the ensilage Kaifeng, as can be seen from Table 2, ensilage pH value and moisture are suitable, and smell is good, color is bright, quality is soft, reaches the quality requirements of excellent ensilage.
Claims (6)
1. banana microbial additive for silage fodder, it is characterized in that: described microbe additive is comprised of Trichoderma viride, candida utili and plant lactobacillus and carrier.
2. microbe additive according to claim 1 is characterized in that: described plant lactobacillus separates from sauerkraut, screen and obtain.
3. microbe additive claimed in claim 1, it is characterized in that: described Trichoderma viride: candida utili: the volume ratio of plant lactobacillus is 1:2:1.
4. microbe additive according to claim 1 is characterized in that: the carrier of described microbe additive is the solid medium of banana stem leaves powder and the preparation of auxiliary material cane molasses.
5. the preparation method of microbe additive according to claim 1: described plant lactobacillus dilution spread and line partition method by routine from banana garden soil, commercially available sauerkraut and other feed addictives filters out the plant lactobacillus that is suitable for the banana stem leaves ensiling; Plant lactobacillus, Trichoderma viride and the candida utili of screening are cultivated with MRS, PDA and brewer's wort culture medium slant respectively first, enlarged with fluid nutrient medium respectively again and cultivate; Be linked into after by volume ratio 1:2:1 mixes with plant lactobacillus, Trichoderma viride and candida utili nutrient solution after enlarge cultivating that 27 ℃ ~ 30 ℃ airtight cultivations were prepared into solid additive in 3 ~ 5 days in the solid medium of banana stem leaves powder and the preparation of auxiliary material cane molasses.
6. microbe additive according to claim 5, it is characterized in that: microbe additive is solid, need be watered rear use when being used for the banana stem leaves ensiling, the pH value of ensilage was between 4 ~ 5 after ensiling was finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104037519A CN102907572A (en) | 2012-10-22 | 2012-10-22 | Banana silage microbial additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104037519A CN102907572A (en) | 2012-10-22 | 2012-10-22 | Banana silage microbial additive and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102907572A true CN102907572A (en) | 2013-02-06 |
Family
ID=47606335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104037519A Pending CN102907572A (en) | 2012-10-22 | 2012-10-22 | Banana silage microbial additive and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102907572A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404745A (en) * | 2013-08-29 | 2013-11-27 | 南宁欣欣壮德农牧科技有限公司 | Overhead-price pellet feed for goats in captivity |
| CN103598420A (en) * | 2013-11-20 | 2014-02-26 | 广西壮族自治区农业科学院农产品加工研究所 | Compound microorganism bacterium agent for banana stem and leaf silage and preparation method of compound microorganism bacterium agent |
| CN103652355A (en) * | 2013-11-27 | 2014-03-26 | 广西武宣金泰丰农业科技发展有限公司 | Method utilizing banana stems and leaves to manufacture biological feed for beef cattle |
| CN103689254A (en) * | 2013-11-27 | 2014-04-02 | 广西武宣金泰丰农业科技发展有限公司 | Method for manufacturing biological feed for beef cattle from sugarcane tail |
| CN104322438A (en) * | 2014-09-29 | 2015-02-04 | 河口鑫利畜牧产业有限公司 | Method for breeding cattle by using banana stems and leaves |
| CN104585481A (en) * | 2015-02-13 | 2015-05-06 | 南通大学 | Method for adding lucid ganoderma to assist fermentation of straw silage feed |
| CN104799129A (en) * | 2015-05-22 | 2015-07-29 | 浦北县科学技术开发中心 | Method for preparing pork pig feed by adopting banana leaf sheath as main raw material |
| CN104814356A (en) * | 2015-05-22 | 2015-08-05 | 浦北县科学技术开发中心 | Method for preparing pork pig feed by taking banana cauloid as main raw material |
| CN105505994A (en) * | 2015-12-16 | 2016-04-20 | 清华大学 | Comprehensive utilizing method of banana waste |
| CN106490344A (en) * | 2016-01-13 | 2017-03-15 | 中弘新缘生物科技（天津）有限公司 | A kind of hybrid giant napier silage inoculants bacteria and preparation method thereof |
| CN108835374A (en) * | 2018-08-03 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | One kind being rich in carrotene feed and its processing method |
| CN109730192A (en) * | 2019-03-06 | 2019-05-10 | 广西壮族自治区农业科学院 | Utilize the method for bagasse production protein feed |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754961A (en) * | 2005-09-13 | 2006-04-05 | 新疆大学 | Production of ethyl alcohol and micro-fermentation feedstuff by sorgo straw |
| CN1759703A (en) * | 2005-10-10 | 2006-04-19 | 新疆大学 | Method for producing beverage of lactic acid and tiny storable feedstuff through stalk of sorgo |
| CN101248845A (en) * | 2008-03-28 | 2008-08-27 | 成都枫澜科技有限公司 | Method for processing raw material of aquatic product feed stuff by fermenting |
| CN101313728A (en) * | 2008-05-17 | 2008-12-03 | 邹宗森 | Stalk biochemistry forage grass and its processing method |
| CN101579041A (en) * | 2009-06-14 | 2009-11-18 | 中国热带农业科学院热带作物品种资源研究所 | Preparation method of biological feed of banana stem leaves |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102449156A (en) * | 2009-03-17 | 2012-05-09 | 全技术公司 | Compositions and methods for conversion of lignocellulosic material to fermentable sugars and products produced therefrom |
-
2012
- 2012-10-22 CN CN2012104037519A patent/CN102907572A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754961A (en) * | 2005-09-13 | 2006-04-05 | 新疆大学 | Production of ethyl alcohol and micro-fermentation feedstuff by sorgo straw |
| CN1759703A (en) * | 2005-10-10 | 2006-04-19 | 新疆大学 | Method for producing beverage of lactic acid and tiny storable feedstuff through stalk of sorgo |
| CN101248845A (en) * | 2008-03-28 | 2008-08-27 | 成都枫澜科技有限公司 | Method for processing raw material of aquatic product feed stuff by fermenting |
| CN101313728A (en) * | 2008-05-17 | 2008-12-03 | 邹宗森 | Stalk biochemistry forage grass and its processing method |
| CN102449156A (en) * | 2009-03-17 | 2012-05-09 | 全技术公司 | Compositions and methods for conversion of lignocellulosic material to fermentable sugars and products produced therefrom |
| CN101579041A (en) * | 2009-06-14 | 2009-11-18 | 中国热带农业科学院热带作物品种资源研究所 | Preparation method of biological feed of banana stem leaves |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404745B (en) * | 2013-08-29 | 2014-12-03 | 南宁欣欣壮德农牧科技有限公司 | Overhead-price pellet feed for goats in captivity |
| CN103404745A (en) * | 2013-08-29 | 2013-11-27 | 南宁欣欣壮德农牧科技有限公司 | Overhead-price pellet feed for goats in captivity |
| CN103598420A (en) * | 2013-11-20 | 2014-02-26 | 广西壮族自治区农业科学院农产品加工研究所 | Compound microorganism bacterium agent for banana stem and leaf silage and preparation method of compound microorganism bacterium agent |
| CN103689254B (en) * | 2013-11-27 | 2015-04-15 | 广西武宣金泰丰农业科技发展有限公司 | Method for manufacturing biological feed for beef cattle from sugarcane tail |
| CN103689254A (en) * | 2013-11-27 | 2014-04-02 | 广西武宣金泰丰农业科技发展有限公司 | Method for manufacturing biological feed for beef cattle from sugarcane tail |
| CN103652355A (en) * | 2013-11-27 | 2014-03-26 | 广西武宣金泰丰农业科技发展有限公司 | Method utilizing banana stems and leaves to manufacture biological feed for beef cattle |
| CN103652355B (en) * | 2013-11-27 | 2015-11-18 | 广西武宣金泰丰农业科技发展有限公司 | Banana stem leaves is utilized to make the method for stocker biological feedstuff |
| CN104322438A (en) * | 2014-09-29 | 2015-02-04 | 河口鑫利畜牧产业有限公司 | Method for breeding cattle by using banana stems and leaves |
| CN104585481A (en) * | 2015-02-13 | 2015-05-06 | 南通大学 | Method for adding lucid ganoderma to assist fermentation of straw silage feed |
| CN104799129A (en) * | 2015-05-22 | 2015-07-29 | 浦北县科学技术开发中心 | Method for preparing pork pig feed by adopting banana leaf sheath as main raw material |
| CN104814356A (en) * | 2015-05-22 | 2015-08-05 | 浦北县科学技术开发中心 | Method for preparing pork pig feed by taking banana cauloid as main raw material |
| CN105505994A (en) * | 2015-12-16 | 2016-04-20 | 清华大学 | Comprehensive utilizing method of banana waste |
| CN106490344A (en) * | 2016-01-13 | 2017-03-15 | 中弘新缘生物科技（天津）有限公司 | A kind of hybrid giant napier silage inoculants bacteria and preparation method thereof |
| CN108835374A (en) * | 2018-08-03 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | One kind being rich in carrotene feed and its processing method |
| CN109730192A (en) * | 2019-03-06 | 2019-05-10 | 广西壮族自治区农业科学院 | Utilize the method for bagasse production protein feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102907572A (en) | Banana silage microbial additive and preparation method thereof | |
| CN102864095B (en) | Lactobacillus plantarum used for silage alfalfa and use method thereof | |
| CN105002116B (en) | A kind of microbial straw feed microbial inoculum and preparation method thereof | |
| CN107603924A (en) | A kind of complex microorganism preparations and its preparation method and application | |
| CN103598420B (en) | Compound microorganism bacterium agent for banana stem and leaf silage and preparation method of compound microorganism bacterium agent | |
| CN107828699A (en) | A kind of farm composite bacteria preparation and preparation method | |
| CN102696860B (en) | Highly efficient and low-cost microbiological feed proteins based on vinegar residue and miscellaneous meal | |
| CN102344326B (en) | Organic biofertilizer capable of inhibiting continuous cropping obstacle of flue-cured tobaccos | |
| CN105918615B (en) | Large-scale production method of rice and wheat straw microbial feed | |
| CN105918614A (en) | Integration processing method of high-efficient corn straw biological feed | |
| CN106635911A (en) | Composite strain fermentation agent and preparation method of silage | |
| CN109022333A (en) | A kind of preparation method and applications of composite microbial fermentation bacteria agent | |
| CN101946853A (en) | Method for preparing silage feed by fermenting straw through composite microbes | |
| CN114586890A (en) | Method for preparing composite flora feed by utilizing juncao | |
| CN102406068B (en) | Method for preparing microbial fermented feed capable of keeping intestinal health of pigs | |
| CN106889306A (en) | A kind of feed fermentation agent and the application on feed fermentation | |
| CN101869230B (en) | Microbial additive for alfalfa haylage, preparation method and application thereof | |
| CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN104694431B (en) | One plant of lactobacillus plantarum（Lactobacillus plantarum）And its application | |
| CN102994421B (en) | Lactic acid bacteria suitable for ensiling oat and applications thereof | |
| CN102815973A (en) | Preparation method of biological selenium-rich fertilizer | |
| CN109666597A (en) | A kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed | |
| CN101965906A (en) | Preparation method of stylosanthes guianensis biological feed | |
| CN1324980C (en) | Method for preparing microoganism additives for ensiling lucerne | |
| CN102450525A (en) | Leguminous forage silage complex bacteria additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Zhichun Inventor after: Pan Jieke Inventor after: Teng Lidian Inventor after: You Xiangrong Inventor after: Zhang Yayuan Inventor after: Sun Jian Inventor after: Li Li Inventor after: Li Changbao Inventor after: Liao Fen Inventor after: Li Jiemin Inventor after: Fu Guiming Inventor before: Li Zhichun Inventor before: Pan Jieke Inventor before: Teng Lidian Inventor before: You Xiangrong Inventor before: Zhang Yayuan Inventor before: Sun Jian Inventor before: Li Li Inventor before: Li Changbao Inventor before: Liao Fen Inventor before: Li Jiemin Inventor before: Gui Ming |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LI ZHICHUN YOU XIANGRONG ZHANG YAYUAN SUN JIAN LI LI LI CHANGBAO LIAO FEN LI JIEMIN GUI MING PAN JIEKE TENG LIDIAN TO: LI ZHICHUN YOU XIANGRONG ZHANG YAYUAN SUN JIAN LI LI LI CHANGBAO LIAO FEN LI JIEMIN FU GUIMING PAN JIEKE TENG LIDIAN |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130206 |