CN102815973A - Preparation method of biological selenium-rich fertilizer - Google Patents
Preparation method of biological selenium-rich fertilizer Download PDFInfo
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- CN102815973A CN102815973A CN2011101501422A CN201110150142A CN102815973A CN 102815973 A CN102815973 A CN 102815973A CN 2011101501422 A CN2011101501422 A CN 2011101501422A CN 201110150142 A CN201110150142 A CN 201110150142A CN 102815973 A CN102815973 A CN 102815973A
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Abstract
The invention relates to a preparation method of a biological selenium-rich fertilizer. According to the invention, various microbes with specific functions are respectively cultured or are combined and cultured, such that a large amount of selenium-rich living cells are obtained; at a same time, a combined culturing product of various probiotics is fermented under an anaerobic condition, such that a large amount of selenium-rich multi-strain fermentation product is obtained; the products are blended according to a certain ratio, such that the fertilizer is obtained. The fertilizer is characterized in multiple microbe floras, multiple functions, multiple metabolites, stable selenium element content, and substantial application effect. Through foliar fertilization, selenium nutrient contents in various crops can be increased, and agricultural product qualities can be improved. The fertilizer is suitable for spray applications on crops such as melons, leafy vegetables, tobaccos, teas, fruit trees, grains, beans, and the like.
Description
Technical field
The present invention is specifically related to a kind of preparation method of biological selenium-rich fertilizer, belongs to the technical field that fertilizer is made, and is to adopt multiple functional microbial and pre-biotic microorganism to combine with the inorganic selenium element, prepares the method for biological selenium-rich fertilizer.
Background technology
China lacks one of the most serious country of selenium in the world, has 72% area to be in the serious selenium area that lacks.Comprise main grain districts such as northeast, North China, southwest.The grain, fruits and vegetables 95% selenium content that cause us to eat now all do not reach international minimum standard.Selenium is the trace element that needed by human obtains from food, and it is healthy that food lacks the selenium direct relation person.Add up according to WHO; One of country that sickness rate such as China's hepatitis, mellitus, cardiovascular diseases, cancer are the highest, relevant with scarce selenium, just the contriver knows; At present through leaf dressing; Making various farm crop contain the selenium nutrient increases, and improves the preparation method of the biological selenium-rich fertilizer of quality of agricultural product, does not also have relevant report.
Summary of the invention
The object of the invention provides a kind of through leaf dressing exactly for overcoming above deficiency, and making various farm crop contain the selenium nutrient increases, and improves a kind of preparation method of biological selenium-rich fertilizer of the biological selenium-rich fertilizer of quality of agricultural product,
Main technical schemes of the present invention is achieved in that the preparation method of described a kind of biological selenium-rich fertilizer, it is characterized in that comprising the preparation of function bacterial classification, the cultivation of probiotic bacterium bacterial classification and contain the design of selenium element substratum.Take aerobic cultivation and anaerobism to cultivate dual mode respectively according to the physilogical characteristics of bacterial classification, obtain the living bacterial cells and the meta-bolites of maximum quantity.Described bacterial classification comprises bacillus, streptomyces, Azotobacter, rhizobium, Saccharomycodes, genus lactubacillus, 7 genus of acetic acid Pseudomonas, the mikrobe of totally 15 kinds.Have fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth and various active enzyme and secondary metabolite, like multiple organic acid, small-molecular peptides, VITAMINs, oligosaccharide substance.Can improve crop alimentary effectively, improve resistance, improve quality, increase output.
Described aerobic training method is to adopt the general deep liquid culture tank of fermentation industry, selects the substratum of suitable growth according to the needs of each bacterial classification, and aeration condition is cultivated, and thalline reaches maximum quantity, and maturation is put jar.
Described anaerobism training method is to adopt deep liquid to leave standstill cultivation, selects appropriate media according to the needs of each bacterial classification, mainly is under anaerobic to make its fermentation, obtains the tunning of maximum.
The slant medium that said process for preparing strain thereof is used has following component and preferable weight proportion: peptone 8～12g Carnis Bovis seu Bubali cream 4～6g glucose 1～3g agar 12～20g zero(ppm) water 1L.
The substratum that described bacterial classification liquid culture is used has following component and preferable weight proportion:
Soybean hydrolyzate 10～30g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g inorganic selenium, 10～100ppm water 1L.
Described bacterial classification deep liquid leaves standstill the substratum of cultivating usefulness, has following component and preferable weight proportion:
Sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10gMgSO
41～6g inorganic selenium, 10～100ppm water 1L.
Positively effect of the present invention is: with the multiple specific function of aerobic training method acquisition; Like: fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth etc.; Be rich in the active somatic cell of organoselenium and fermentation under anaerobic in a large number; The a large amount of multi-strain fermentation product that obtains like: multiple organic acid, small-molecular peptides, VITAMINs, oligosaccharides with through the materials such as organoselenium of bio-transformation, proportions.Obviously improve the multiple effect of product, promptly improved crop alimentary, improved resistance, improved quality, increased output, and satisfied the demand of selenium element.
Production technique:
(1) the aerobic cultivation of bacterial classification comprises the steps:
Slant strains: the test tube slant is in 25-32 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: the test tube slant is poured sterilized water into, scrapes off lawn, the preparation suspension.
Liquid nutrient medium: the substratum that the bacterial classification liquid culture is used has following component and preferable heavy
Amount proportioning: soybean hydrolyzate 10～30g peptone 4～10g sucrose 16～24gKH
2PO
43～8g MgSO
41～6g inorganic selenium, 10～100ppm water 1L.
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilizing 30 minutes.
Inoculation: bacteria suspension is inserted in the substratum of sterilization.
Cultivate: 25-32 ℃, tank pressure 0.04-0.05 cultivates 36-72h.
1. culture compound: will cultivate the nutrient solution of acquisition respectively, and mix, and stir.
(2) the bacterial classification deep liquid leaves standstill cultivation:
Slant strains: the test tube slant is in 25-32 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: the test tube slant is poured sterilized water into, scrapes off lawn, the preparation suspension.
Liquid nutrient medium: sucrose 10～30g peptone 4～10g yeast extract paste 5-10gKH
2PO
45～10g MgSO
41～6g inorganic selenium, 10～100ppm water 1L
Sterilization: 115 ℃, steam sterilizing 20 minutes.
Inoculation: bacteria suspension is inserted in the substratum of sterilization.
Cultivate: 25-32 ℃, cultivate 36-72h.
2. culture compound: will cultivate the nutrient solution of acquisition respectively, and mix, and stir.
(3) culture is compound 1. compound 2. compound with culture: two kinds of mixtures are mixed, stir, be the liquor product.
The present invention is compared with prior art: the present invention is at first with the mikrobe of multiple specific function, respectively or compound cultivation obtain a large amount of rich selenium active somatic cells, meanwhile; With the compound culture of multiple probiotic bacterium, under anaerobic ferment, obtain the multi-strain fermentation product of a large amount of rich selenium; Proportion then, many with making it have a microbe groups, function is many; Meta-bolites is many, and it is stable to contain the selenium element, the tangible advantage of effect.
Embodiment
Embodiment 1
1) with the bacterial classification of fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth function, is inoculated on the test tube slant respectively.The test tube slant places 25-32 ℃ of constant temperature culture, 24-72h.
2) cultured test tube slant is poured sterilized water into, and scrapes and get lawn and prepare bacteria suspension in aseptic bottle.
3) obtaining liq substratum, its component is: soybean hydrolyzate 10～30g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g inorganic selenium 10-100PPM water 1L.
4) steam sterilizing, 0.12-0.15Mpa, 121-125 ℃, 30 minutes.
5) bacteria suspension is inserted in the substratum of sterilization.
6) 25-37 ℃, cultivate 36-72h.
7) will cultivate the nutrient solution of acquisition respectively, mix, stir.Be compound 1. liquid.
8) will be used for the bacterial classification that deep layer leaves standstill cultivation, respectively activation in the sterilization bottle.
9) leave standstill the liquid nutrient medium of cultivation, its component is: sucrose 10～30g peptone 4～10g yeast extract paste 5-10g KH
2PO
45～10g MgSO
41～6g inorganic selenium 10-100PPM water 1L
10) above-mentioned cultivation is based on 115 ℃, steam sterilizing 20 minutes.
11) the activatory bacteria suspension is inserted in the substratum, 25-37 ℃, cultivated 2-7 days.Be compound 2. liquid.
12) 2. culture compound 1. compound with culture, and two kinds of mixtures mix, and stir, and are the liquor product.
100 times of this product dilutions spray, vegetable category raising the output 10-25%, and leaf vegetables is as leading to heart dish up to 40%.Cigarette, tea, fruit tree all have obvious effect of increasing production.Experiment showed, grain, beans raising the output 5-15%.These article selenium content of crops of spraying reaches the normal requirement 25-40PPM. of human body
This product safety is reliable, and is to people, animal, plant soil, nontoxic, have no side effect environmentally safe.Consumption is few, cost is low, use convenience, and is safe and reliable.
Claims (6)
1. the preparation method of a biological selenium-rich fertilizer; Comprise the preparation of function bacterial classification; The cultivation of probiotic bacterium bacterial classification; With the design that contains selenium element substratum, it is characterized in that taking aerobic cultivation and anaerobism to cultivate dual mode respectively according to the physilogical characteristics of bacterial classification, obtain the living bacterial cells and the meta-bolites of maximum quantity.In yeast culture, accomplish of the conversion of inorganic selenium element to organoselenium.
2. the preparation method of a kind of biological selenium-rich fertilizer according to claim 1; It is characterized in that the preparation of function bacterial classification; Employing has the mikrobe of physiological properties such as fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth, selects aerobic training method, in the culture medium culturing that contains the selenium element.
3. the preparation method of a kind of biological selenium-rich fertilizer according to claim 2 is characterized in that having in the preparation substratum of function bacterial classification the ratio of soybean hydrolyzate and each component: soybean hydrolyzate 10～50g peptone 4～10g sucrose 16～24g KH
2PO
43～8g MgSO
41～6g selenium 10-100PPM water 1L.
4. the preparation method of a kind of biological selenium-rich fertilizer according to claim 1; The cultivation that it is characterized in that the probiotic bacterium bacterial classification adopts bacterial classification in vital movement; Can produce various active enzyme and secondary metabolite, like the mikrobe of multiple organic acid, small-molecular peptides, VITAMINs, oligosaccharide substance.The anaerobism training method of selecting deep layer to leave standstill is in the culture medium culturing that contains the selenium element.
5. the preparation method of a kind of biological selenium-rich fertilizer according to claim 4; It is characterized in that having following component and weight proportion in the anaerobic culture medium that deep layer leaves standstill: sucrose 10-30g peptone 4-10g, yeast extract paste 5-10g KHPO 5-1Og MgSO 1-6g selenium 10-100PPM water 1L.
6. according to the preparation method of claim 1,2,3,4 or 5 described a kind of biological selenium-rich fertilizers; The cultivation that it is characterized in that multiple mikrobe adopts many bacterial classifications to select aerobic training method and anaerobism training method; The culture that is obtained proportions, and its ratio is 1: 2-5.
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| CN2011101501422A CN102815973A (en) | 2011-06-07 | 2011-06-07 | Preparation method of biological selenium-rich fertilizer |
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| CN2011101501422A CN102815973A (en) | 2011-06-07 | 2011-06-07 | Preparation method of biological selenium-rich fertilizer |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103626587A (en) * | 2013-12-03 | 2014-03-12 | 广西新方向化学工业有限公司 | Preparation of sugar alcohol organic selenium-rich foliar fertilizer by fermentation method |
| CN103708960A (en) * | 2013-12-24 | 2014-04-09 | 安徽师范大学 | Liquid-state compound-microorganism bacterial fertilizer as well as production method and application thereof |
| CN104609924A (en) * | 2015-01-19 | 2015-05-13 | 安徽大学 | Preparation method and application of special nutrient solution for blueberry rhizospheric microorganism activation |
| CN105601363A (en) * | 2016-02-04 | 2016-05-25 | 王植 | Selenium-enriched fertilizer additive, selenium-enriched fertilizer and preparation method thereof |
| CN108046932A (en) * | 2018-01-18 | 2018-05-18 | 曲秋虹 | A kind of production technology of organism organic selenium fertilizer |
| CN108358725A (en) * | 2017-12-26 | 2018-08-03 | 张渝钢 | A kind of Se-enriched bio organic fertilizer and its preparation method and application |
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| CN1556070A (en) * | 2003-12-31 | 2004-12-22 | 南京新世纪园艺研究所 | Selenium enriched liquid fertilizer and public hazard less production method of selenium enriched aqgricultural product thereof |
| CN1594234A (en) * | 2004-07-05 | 2005-03-16 | 吴敦虎 | Organic selenium biologics and its preparation method, and method for producing selenium-rich vegetable using the same |
| KR20050032050A (en) * | 2005-02-14 | 2005-04-06 | 김현채 | Culture method of chlorella containing selenium and germanium |
| KR100728585B1 (en) * | 2005-05-16 | 2007-06-15 | 주식회사 메디오젠 | lactic ferments with selenium and manufacturing method thereof |
| CN101054315A (en) * | 2007-05-08 | 2007-10-17 | 李学珍 | Selenium-rich zoology whole-element fertilizer and producing method thereof |
| CN101054564A (en) * | 2007-04-09 | 2007-10-17 | 崔澎 | Selenium-rich combination azotobacter and preparation method for freezing dry powder, liquid fertilizer and solid fertilizer of the same |
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2011
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1556070A (en) * | 2003-12-31 | 2004-12-22 | 南京新世纪园艺研究所 | Selenium enriched liquid fertilizer and public hazard less production method of selenium enriched aqgricultural product thereof |
| CN1594234A (en) * | 2004-07-05 | 2005-03-16 | 吴敦虎 | Organic selenium biologics and its preparation method, and method for producing selenium-rich vegetable using the same |
| KR20050032050A (en) * | 2005-02-14 | 2005-04-06 | 김현채 | Culture method of chlorella containing selenium and germanium |
| KR100728585B1 (en) * | 2005-05-16 | 2007-06-15 | 주식회사 메디오젠 | lactic ferments with selenium and manufacturing method thereof |
| CN101054564A (en) * | 2007-04-09 | 2007-10-17 | 崔澎 | Selenium-rich combination azotobacter and preparation method for freezing dry powder, liquid fertilizer and solid fertilizer of the same |
| CN101054315A (en) * | 2007-05-08 | 2007-10-17 | 李学珍 | Selenium-rich zoology whole-element fertilizer and producing method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103626587A (en) * | 2013-12-03 | 2014-03-12 | 广西新方向化学工业有限公司 | Preparation of sugar alcohol organic selenium-rich foliar fertilizer by fermentation method |
| CN103708960A (en) * | 2013-12-24 | 2014-04-09 | 安徽师范大学 | Liquid-state compound-microorganism bacterial fertilizer as well as production method and application thereof |
| CN103708960B (en) * | 2013-12-24 | 2016-06-01 | 安徽师范大学 | A kind of liquid composite microbic bacterial fertilizer and production method and purposes |
| CN104609924A (en) * | 2015-01-19 | 2015-05-13 | 安徽大学 | Preparation method and application of special nutrient solution for blueberry rhizospheric microorganism activation |
| CN105601363A (en) * | 2016-02-04 | 2016-05-25 | 王植 | Selenium-enriched fertilizer additive, selenium-enriched fertilizer and preparation method thereof |
| CN108358725A (en) * | 2017-12-26 | 2018-08-03 | 张渝钢 | A kind of Se-enriched bio organic fertilizer and its preparation method and application |
| CN108046932A (en) * | 2018-01-18 | 2018-05-18 | 曲秋虹 | A kind of production technology of organism organic selenium fertilizer |
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Application publication date: 20121212 |