CN105948831B - A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains - Google Patents
A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
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Abstract
It is the method for main material production fungi biological and ecological methods to prevent plant disease, pests, and erosion fertilizer that the present invention relates to a kind of using brewex's grains, belongs to microbial pesticide and agricultural fertilizer technical field, and the biocontrol fungi strain is beauveria bassiana (Beauveria bassiana).The invention further relates to the production method of this kind of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, mainly include 4 steps: seed preparation, semi-solid ferment, solid state fermentation, post-processing technology, wherein semisolid and solid state fermentation with brewex's grains for main carbon nitrogen source and carrier, prepare solid biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.The present invention is not only that the recycling of brewex's grains waste provides a new way, and the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer produced can promote plant growth, have preferable control efficiency to various plants pest.
Description
Technical field
The invention belongs to agricultural fertilizer technical fields, and in particular to a kind of microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer and preparation method thereof, it is special
It is not a kind of high-effective microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of pest-resistant growth-promoting.
Background technique
With the development of China's economic, as the in the world first big alcoholic beverage, the beer consumption in China also gradually adds
Greatly.According to statistics, Chinese beer yield in 2014 is up to 4921.85 ten thousand kilolitres, is maximum Beer Brewage state in the world.According to statistics,
1 ton of beer of every production, needs to generate about 0.25 ton of brewex's grains.Brewex's grains are mainly beer raw material malt, rice and hops warp
After crossing saccharification gelatinization process, it is filtered the waste material that squeezing obtains.Moisture content in brewex's grains is about 50%~85%, simultaneously
Nutritional ingredient rich in, wherein crude protein accounts for about the 25% of dry weight, crude fat about 10%, crude fibre about 8%, nitrogen-free leaching
The ash content of object about 55% and 2% out.It is full of nutrition but since wherein moisture content is higher, so being easy to occur rotten
It loses.Currently, feed of the brewex's grains mostly by direct sale at low prices to surrounding peasants, as stock raising；Another part is direct by it
It is dried, then carries out subsequent feed processing processing, energy consumption is excessive；What is more by its direct emission, serious dirt
Contaminate environment.It would therefore be highly desirable to be developed and utilized for brewex's grains, the further added value for improving brewex's grains.
Biocontrol fungi be it is a kind of can invade insect endobiosis, insect is made to fall ill lethal fungi, be a kind of extremely important
Environmental microorganism, prevention and treatment harmful organism in have wide development potentiality.Beauveria bassiana (Beauveria
Bassiana) be a kind of broad spectrum activity biocontrol fungi, effective ingredient be include that spore or mycelia infect body, generally from pest
Body wall invades haemocoele, breeds or excretes poison using nutrition in host's haemocoele, and pest is because of nutrition failure or death due to toxaemia.
Researchers at home and abroad utilize beauveria bassiana prevention and treatment corn borer, pine moth, small sugarcane borer, blind Chinese toon, grain weevil, citrus red mite and aphid
The agriculture and forestry injurious insects such as worm.Especially to the biological control of corn borer and pine moth, it has been used as conventional means to use year after year at home.By
Population number can be efficiently controlled in beauveria bassiana, while not injuring other natural enemy insects and beneficial organism, has been complied fully with
The objective of evil biotechnology, simultaneously because it is easy mass production, control cost is more competitive, thus it has extensively
Application prospect.In addition, it has been investigated that, beauveria bassiana is red to sclerotinia sclerotiorum germ, cotton wilt germ and wheat
The pathogens such as mildew have certain antagonism.
Being disclosed patent in the prior art is " a kind of solid medium of muscardine and the cultural method of muscardine ", public
The number of opening is 103881930A.Wherein, the solid medium of the invention includes 1:(1~1.2 in mass ratio): the leech of (1~1.2)
Stone, wheat bran and corn flour obtain vermiculite, wheat bran and corn flour mixed vapour sterilizing 40min；On this basis, the hair
It is bright by muscardine inoculation liquid after the fluid enlargement culture base culture added with gentamicin sulphate, be inoculated in solid medium
In, it is harvested after 25~27 DEG C of 7.5~8d of culture；In addition to this tail feather, Chen Hongmei etc. are also thanked, it is " raw using rice solid state fermentation
Produce the process optimization of beauveria bassiana " food and fermentation industries, 2007,33 (10).Wherein, which is sent out using rice as solid-state
Ferment culture medium, inoculum concentration are 15% (v/w), and 25 DEG C of cultivation temperature, 4d is 100% before ambient humidity, and intermediate 3d is 80%, rear 3d
It is 60%, cultivates 10d.
In above-mentioned patent and paper, the edible agricultural product of the people and animals such as rice, wheat bran, corn flour have largely been used, have been increased
The consumption of grain resource；Solid culture based component therein is many and diverse, higher cost；Produced using beauveria bassiana spore powder as final
Object need to carry out many and diverse operating procedure and individually be separated and recovered to conidia powder, and original solid culture Quito is by again
It is discarded；And it is not directed to the fertilizer field of plant growth-promoting.
Therefore, biocontrol fungi how is cultivated using a large amount of brewex's grains as urgent problem to be solved.
Summary of the invention
It is a kind of raw using beer useless grain the technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide
The method for generating anti-fertilizer.
The brewex's grains waste material that the present invention is generated using beer fermentation is culture medium, the significant white deadlock of ball spore of culture insecticidal power
Bacterium, and the fertilizer after fermentation has richer nutriment, can effectively facilitate the growth of plant, while realizing to plant
The prevention and treatment of insect pest, food-safety problem caused by reducing chemical pesticide during planting.
The specific technical solution of the present invention is as follows:
A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains, it is characterised in that: specific step is as follows:
(1) prepared by seed: beauveria bassiana being accessed in SADY fluid nutrient medium, inoculum concentration is 1~3%, 25~28
DEG C, 3~7d is cultivated in the shaking table of 150~180r/min, obtains seed liquor；
(2) semi-solid ferment: will be added semi-solid ferment in fermentor, consisting of: the young beer of 50~150g
Grain, 5~40g glucose are settled to 1L with water.15~30min is boiled in heating, to eliminate miscellaneous bacteria in culture medium.It is cooled to room
Wen Hou is inoculated with 0.5~5% seed liquor, in 25~28 DEG C of ventilation 24~72h of aerobic culture, obtains semi-solid ferment culture solution；
(3) solid state fermentation: taking the original sugarcane Icing Sugar that young beer is poor, and addition accounts for 0.5~2wt% of brewex's grains to mix well,
In 80 DEG C of 30~45min of baking, brewex's grains humidity is reduced to 50~65%, cooling is spare；
The biocontrol fungi semisolid culture solution obtained in step (2) is added to step (3) according to 10~20% ratio
In the solid medium of middle acquisition, it is uniformly mixed；By the solid medium after inoculation, it is put in 25~28 DEG C of isoperibols and seals
8~15d is cultivated, 3~5d of desiccation culture is then aerated, is lower than 15% to moisture content in medium, and there are a large amount of muscardines
When conidia powder, purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer can be obtained.
Disinfecting action can be realized in 80 DEG C of 30~45min of baking in brewex's grains in this step；
(Beauveria bassiana) strain of beauveria bassiana described in step (1) is commercially available beauveria bassiana microbial inoculum,
As " general " beauveria bassiana of Jiangxi Tianren Ecology Co., Ltd., Xiamen the more Biotechnology Co., Ltd it is " more
More biologies " beauveria bassiana etc..
SADY fluid nutrient medium described in step (1) is the preparation method is as follows: glucose 4g, peptone 1g, yeast leach
Powder 1g is settled to 100mL with water, after high-temperature sterilization, is cooled to room temperature spare.
Since the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer obtained in step (3) is bulk, in order to facilitate use can by blocking biological and ecological methods to prevent plant disease, pests, and erosion fertilizer into
Row break process can be obtained biological and ecological methods to prevent plant disease, pests, and erosion fertilizer finished product.
The invention has the benefit that
1. biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the invention is to be formed by brewex's grains through fermentation of white muscardine fungi, it can effectively improve soil micro-ecosystem
Environment, killing off plant insect pest, nutriment needed for providing plant growth provide beneficial conditions for crop growth.
2. the culture medium using brewex's grains as biocontrol fungi, main reason is that it both contained as microbiological culture media
The carbon source for thering is residual sugar to grow as microorganism, and the nitrogen source that crude protein rich in is required as microorganism growth, while
Containing microelements such as calcium, phosphorus, manganese, iron, copper needed for microorganism growth, it is one kind without additional addition or need to only adds on a small quantity
The outstanding microbiological culture media of other chemical agents.In addition, after ball embraces fermentation of white muscardine fungi, the thick egg that contains in brewex's grains
White equal substances are gradually decomposed into small molecule inorganic nitrogen, are conducive to plant and are directly absorbed.Meanwhile contain in brewex's grains calcium, phosphorus,
The microelements such as manganese, iron, copper are also nutriment necessary to plant growth.
3. experiment shows that plant seed germination and growth can be promoted using biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the invention, through field experiment
Can be improved grain yield 10~35%, improve yield of vegetables 15~35%, can also effectively improve the qualities of grain vegetables with
Flavor.Land use fertilizer of the invention about 200~300g per acre is the less microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of current amount of application.Tool
100~200 times of water are added when body uses in biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the present invention, 12h is activated in 22~28 DEG C, then together with former applicationization
Fertile (the 50% of normal use amount) is sprinkled into soil.
In conclusion cheap waste material --- the brewex's grains that the present invention effectively will largely generate in beer actual production
Development of resources recycling is carried out, environmental sanitation problem caused by dealing with improperly because of brewex's grains is effectively avoided, meanwhile, into one
The added value for improving brewex's grains of step, provides a new approaches for the high-value-use of brewex's grains；Using brewex's grains as biological and ecological methods to prevent plant disease, pests, and erosion
Fungi culture medium, rather than the edible agricultural product of the people and animals such as rice, wheat bran, corn flour are used, reduce the consumption of grain resource；
By brewex's grains fermenting and producing desinsection strength, strong, environmental-friendly, person poultry harmless the beauveria bassiana of endurance, mesh can be used as
The substitute of the preceding chemical pesticide excessively used, effectively reduces its usage amount, to avoid applying due to chemical pesticide excessive
The food-safety problems such as the environmental pollutions such as caused soil, water source, atmosphere and pesticide residue；The beer that will be obtained after fermentation
Poor beauveria bassiana mixture is simply processed, and conidia powder recycling step is omitted, and has both been had abundant from brewex's grains
Nutriment is a kind of excellent bio-feritlizer but also with the insecticidal effect of biocontrol fungi.
Specific embodiment
The specific embodiment of form by the following examples does further specifically above content of the invention
It is bright, but the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Embodiment 1
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biocontrol fungi seed bacterial strain, it can
Select " general " beauveria bassiana, the limited public affairs of Xiamen the more biotechnology of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of department.Beauveria bassiana is accessed into SADY fluid nutrient medium (glucose 4g, peptone
1g, yeast extract powder 1g are settled to 100mL with water, after high-temperature sterilization, are cooled to room temperature, spare) in, inoculum concentration 3%；?
26 DEG C, 4d is cultivated in the shaking table of 150r/min, obtains seed liquor, it is spare.
2. semi-solid ferment: semi-solid ferment, composition ratio will be added in fermentor are as follows: the young beer of 100g
Grain, 20g glucose are settled to 1L with water, and heating boils 30min, sterilizes to semi-solid ferment, be cooled to room temperature
Afterwards, it is inoculated with 3% seed liquor of semi-solid ferment, for 24 hours in the aerobic culture of 27 DEG C of ventilations, obtains semi-solid ferment culture solution；
3. solid state fermentation: it takes young beer poor, 1wt% original sugarcane Icing Sugar is added and mixes well, in 80 DEG C of drying 45min,
Brewex's grains humidity is reduced to 50%, cooling is spare；
The biocontrol fungi semisolid culture solution of acquisition is added in solid medium according to 20% ratio, is uniformly mixed；It will
Solid medium after inoculation is put in sealing culture 10d in 27 DEG C of isoperibols, culture 5d is then aerated, to culture medium
Water content is down to 15% hereinafter, and there are when a large amount of beauveria bassiana spore powders, can be obtained purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.
4. subsequent processing: blocking biological and ecological methods to prevent plant disease, pests, and erosion fertilizer being carried out break process, can be obtained biological and ecological methods to prevent plant disease, pests, and erosion fertilizer finished product.
Embodiment 2
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biocontrol fungi seed bacterial strain, it can
Select " general " beauveria bassiana, the limited public affairs of Xiamen the more biotechnology of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of department.Beauveria bassiana is accessed into SADY fluid nutrient medium (glucose 4g, peptone
1g, yeast extract powder 1g are settled to 100mL with water, after high-temperature sterilization, are cooled to room temperature, spare) in, inoculum concentration 2%,
25 DEG C, 5d is cultivated in the shaking table of 160r/min, obtains seed liquor, it is spare.
2. semi-solid ferment: semi-solid ferment, composition ratio will be added in fermentor are as follows: the young beer of 150g
Grain, 10g glucose are settled to 1L with water, and heating boils 20min, sterilizes to semi-solid ferment, be cooled to room temperature
Afterwards, it is inoculated with 5% seed liquor of semi-solid ferment, for 24 hours in the aerobic culture of 26 DEG C of ventilations, obtains semi-solid ferment culture solution；
3. solid state fermentation: taking young beer poor, 1.5wt% original sugarcane Icing Sugar is added and mixes well, is dried in 80 DEG C
35min reduces brewex's grains humidity to 60%, and cooling is spare；By the biocontrol fungi semisolid culture solution of acquisition according to 15% ratio
Example is added in solid medium, is uniformly mixed；By the solid medium after inoculation, it is put in 26 DEG C of isoperibols and seals culture
Then 12d is aerated culture 3d, when moisture content in medium be down to 15% hereinafter, and there are when a large amount of beauveria bassiana spore powders, i.e.,
It can get purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.
4. subsequent processing: blocking biological and ecological methods to prevent plant disease, pests, and erosion fertilizer being carried out break process, can be obtained biological and ecological methods to prevent plant disease, pests, and erosion fertilizer finished product.
Embodiment 3
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biocontrol fungi seed bacterial strain, it can
Select " general " beauveria bassiana, the limited public affairs of Xiamen the more biotechnology of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of department.Beauveria bassiana is accessed into SADY fluid nutrient medium (glucose 4g, peptone
1g, yeast extract powder 1g are settled to 100mL with water, after high-temperature sterilization, are cooled to room temperature, spare) in, inoculum concentration 2%,
25 DEG C, 5d is cultivated in the shaking table of 160r/min, obtains seed liquor, it is spare.
2. semisolid state is fermented: semi-solid ferment, composition ratio will be added in fermentor are as follows: the young beer of 150g
Grain, 10g glucose are settled to 1L with water, and heating boils 20min, sterilizes to semi-solid ferment, be cooled to room temperature
Afterwards, it is inoculated with 5% seed liquor of semi-solid ferment, for 24 hours in the aerobic culture of 26 DEG C of ventilations, obtains semi-solid ferment culture solution；
3. solid state fermentation: taking young beer poor, 1.5wt% original sugarcane Icing Sugar is added and mixes well, is dried in 80 DEG C
35min reduces brewex's grains humidity to 60%, and cooling is spare；By the biocontrol fungi semisolid culture solution of acquisition according to 10% ratio
Example is added in solid medium, is uniformly mixed；By the solid medium after inoculation, it is put in 26 DEG C of isoperibols and seals culture
Then 14d is aerated culture 3d, when moisture content in medium be down to 15% hereinafter, and there are when a large amount of beauveria bassiana spore powders, i.e.,
It can get purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.
4. subsequent processing: blocking biological and ecological methods to prevent plant disease, pests, and erosion fertilizer being carried out break process, can be obtained biological and ecological methods to prevent plant disease, pests, and erosion fertilizer finished product.
The detection of 4 biocontrol fungi sporulation quantity of embodiment
Example 1, embodiment 2, the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer 5g obtained in embodiment 3 are immersed in containing 2wt% Tween-80 respectively
500ml sterile water in, be thoroughly mixed, after 30min, carry out using blood cell after a certain proportion of dilution under the microscope
Tally counts, and calculates its sporulation quantity.It can be obtained by table 1, biocontrol fungi sporulation quantity is more in this biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, and up to 8.9 × 1011
A/g illustrates that being very suitable for biocontrol fungi as solid medium using brewex's grains in the present invention grows, and higher
Biocontrol fungi sporulation quantity is the basic guarantee of insecticidal effect.
1 biological and ecological methods to prevent plant disease, pests, and erosion fertilizer sporulation quantity of table detects table
The detection of 5 biocontrol fungi germination rate of embodiment
2 biological and ecological methods to prevent plant disease, pests, and erosion fertilizer germination rate of table detects table
Example 1, embodiment 2, the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer 1g obtained in embodiment 3 are immersed in 100ml sterile water respectively, are filled
Point stirring, counts under microscope, with sterile water by its strain concentration dilution to 107A/mL.Every group of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer sample sampling 5
It is a, 26 DEG C of constant temperature incubations are respectively placed in for 24 hours, in its germination rate of microscopically observation.Experiment in triplicate, is averaged.By
Table 2 can obtain, this biological and ecological methods to prevent plant disease, pests, and erosion fertilizer generate spore germination rate reach 98% or more, can effective germination and growth, illustrate this solid train
Feeding base will not influence the spore germination of generation, and higher germination rate can effectively ensure that the spore for infecting pest can play
The effect of desinsection.
6 biocontrol fungi virulence determination of embodiment
Example 1, embodiment 2, the 1g biological and ecological methods to prevent plant disease, pests, and erosion fertilizer obtained in embodiment 3 are immersed in 100ml sterile water, are sufficiently stirred
It mixes, is counted under microscope, with sterile water by its strain concentration dilution to 107A/mL.The experiment pair using greater wax moth as virulence determination
As taking greater wax moth larva 200,50 one group, being grouped observation.Every group of greater wax moth is soaked in biological and ecological methods to prevent plant disease, pests, and erosion fertilizer dilution
Middle 10s, taking-up are put in room temperature, observe and record its daily survival number, calculate daily average viability.Experimental result can obtain, this
Biological and ecological methods to prevent plant disease, pests, and erosion fertilizer median lethal time LT50For 3.8d, there is stronger insecticidal toxicity, it being capable of effectively preventing plant insect.
The detection of 7 plant growth-promoting ability of embodiment
Biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is detected to the promotion growth of plant with soybean germination.Take 600 soybean, every 100 conducts
It one group, is observed.Soya seeds are soaked in 10min in 60 ° of alcohol, taking-up washes down.Respectively by embodiment 1, embodiment 2,
The 1g biological and ecological methods to prevent plant disease, pests, and erosion fertilizer and dry beer vinasse obtained in embodiment 3 is immersed in 20ml sterile water, is thoroughly mixed, spare, each
The biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of embodiment does 3 groups of parallel tests.Then soybean is immersed in water, beer according to 1: 3mL ratio respectively
8h in grain, fertilizer leachate is placed in 25~26 DEG C of constant incubators after then taking out, is protected from light culture.Observe and record every group
Soybean is averaged daily plumule shaft length.Experimental result shows that this biological and ecological methods to prevent plant disease, pests, and erosion fertilizer has certain facilitation, energy to soybean growth
The effect of fertilizer for promoting plant growth is enough played, and effect is obvious.
Claims (1)
1. a kind of method using brewex's grains production biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, it is characterised in that: specific step is as follows:
(1) prepared by seed: beauveria bassiana is accessed in SADY fluid nutrient medium, inoculum concentration is 1~3%, and at 25~28 DEG C,
3~7d is cultivated in the shaking table of 150~180r/min, obtains seed liquor；
(2) semi-solid ferment: will be added semi-solid ferment in fermentor, consisting of: the young beer grain of 50~150g, 5
~40g glucose is settled to 1L with water, and 15~30min is boiled in heating, to eliminate miscellaneous bacteria in culture medium, after being cooled to room temperature,
It is inoculated with 0.5~5% seed liquor, in 25~28 DEG C of ventilation 24~72h of aerobic culture, obtains semi-solid ferment culture solution；
(3) solid state fermentation: the original sugarcane Icing Sugar that young beer is poor, and addition accounts for 0.5~2wt% of brewex's grains is taken to mix well, in 80
DEG C 30~45min of baking, reduces brewex's grains humidity to 50~65%, cooling is spare；
The biocontrol fungi semisolid culture solution obtained in step (2) is linked into step (3) according to 10~20% inoculum concentration
In the solid medium of acquisition, it is uniformly mixed；By the solid medium after inoculation, it is put in 25~28 DEG C of isoperibols and seals training
8~15d is supported, 3~5d of desiccation culture is then aerated, is lower than 15% to moisture content in medium, and there are a large amount of muscardine spores
When sub- powder, purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer can be obtained.
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| CN105948831B true CN105948831B (en) | 2019-08-27 |
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| WO2024003323A1 (en) * | 2022-06-29 | 2024-01-04 | Mushlabs Gmbh | Production of a fungal fermentation medium from brewer's spent grain |
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| CN108624512B (en) * | 2018-05-30 | 2021-03-30 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation substrate, preparation method and method for culturing mycorrhiza biological preparation |
| CN113698254A (en) * | 2020-05-07 | 2021-11-26 | 交城县田丰肥业有限公司 | Multi-organic-matter polymerized fertilizer and preparation process thereof |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN115053916A (en) * | 2022-06-29 | 2022-09-16 | 济南大学 | Microbial agent capable of improving stability of beauveria bassiana and preparation method thereof |
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| CN103086760A (en) * | 2013-01-11 | 2013-05-08 | 李继生 | Preparation method of ecological fertilizer |
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| CN103086760A (en) * | 2013-01-11 | 2013-05-08 | 李继生 | Preparation method of ecological fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024003323A1 (en) * | 2022-06-29 | 2024-01-04 | Mushlabs Gmbh | Production of a fungal fermentation medium from brewer's spent grain |
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