CN102718577B - Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate - Google Patents
Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate Download PDFInfo
- Publication number
- CN102718577B CN102718577B CN2012101988137A CN201210198813A CN102718577B CN 102718577 B CN102718577 B CN 102718577B CN 2012101988137 A CN2012101988137 A CN 2012101988137A CN 201210198813 A CN201210198813 A CN 201210198813A CN 102718577 B CN102718577 B CN 102718577B
- Authority
- CN
- China
- Prior art keywords
- grams
- gram
- trichoderma
- bacillus
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates a special organic culturing substrate capable of preventing and treating diseases for peppers and the preparation method of the special organic culturing substrate. The special organic culturing substrate is prepared from the following raw materials: furfural residues, edible fungus residues, oil residues, saw dust, a microbial fermentation fungicide EM composite fungicide, a trichoderma fungicide and a bacillus fungicide, wherein the bacteria liquid of the trichoderma fungicide has the effective bacteria content of 2X10<8>/g-5X10<8>/g, and the bacteria liquid of the bacillus fungicide has the effective bacteria content of 2X10<9>/g-4X10<9>/g. According to the invention, different organic wastes are compounded, are fermented, decomposed and sterilized, then the trichoderma fungicide and the bacillus fungicide which have the function of preventing and treating diseases of pepper roots are added, compositing is carried out, and finally, the special organic culturing substrate is obtained. The special organic culturing substrate prepared by the preparation method contains a large amount of active trichoderma, bacillus and the metabolic products of the active trichoderma and the bacillus, has an obvious inhibition effect on the diseases of the peppers, and improves the quality and the yield of the peppers.
Description
Technical field
The present invention relates to a kind of cultivation matrix and preparation method thereof, particularly a kind of organic culture substrate of preventing and treating Main Diseases in Chili Peppers and preparation method thereof belongs to the cultivation technique without soil field.
Background technology
Capsicum (Chili pepper) is one of China various places vegetable variety of generally cultivating.In recent years, along with the fast development of facility plantation, pepper planting district continuous cropping phenomenon was more and more general, and the disease the lighter who causes because of continuous cropping can make the capsicum underproduction 20%～30%, and the severe one underproduction brings great financial loss to the green pepper farming 50% or more even total crop failure.
Fusarium solani, epidemic disease, damping-off, southern blight, root knot nematode disease etc. are very common fungal diseases during capsicum produces, for many years, not only fail to contain the trend that disease rises take chemical prevention as main measure, but also suppressed the beneficial microorganism in the soil, cause pathogenic bacteria to develop immunity to drugs, cause simultaneously objectionable impurities residual.Therefore, the method for seeking safe and effective control pepper diseases is the basis that promotes capsicum production benign development.
The capsicum soilless culture has the plurality of advantages such as controllability of avoiding soil-borne disease insect pest, continuous cropping obstacle and production, is one of important channel that alleviates pepper diseases.But because above-mentioned pepper diseases is mainly propagated in modes such as seed, seedling replantings, only depend on the change of planting type also to be difficult to the control morbidity.Utilizing the Biocontrol Effect of Trichoderma and genus bacillus, cut off the propagation of Main Diseases in Chili Peppers and what suppress disease is the important measures of soilless culture red pepper high-yield, high-quality by cultivation matrix, also is to solve one of serious approach that occurs of pepper diseases.
Be fit in the situation in conditions such as temperature, the breeding time of capsicum is longer, particularly most of soilless culture capsicum all is to carry out under facility condition, the artificial adjustment of temperature can make its bearing time reach 8-10 months, output is also higher, and this just need to provide good stability, nutritious cultivation matrix.
CN200410023791.6 provides a kind of preparation and application of fruit and vegetable organic substrate type soilless culture substrate, this disclosure of the Invention two substrate formula: the prescription 1: rice husk: the peat composed of rotten mosses: dried poultrymanure=2: 1: 1 (volume ratio); Prescription 2: rice husk: cotton seed hulls: the peat composed of rotten mosses: dried poultrymanure=1: 1: 1: 1 (volume ratio, cotton seed hulls are the waste material of cultivating edible mushrooms).Fully mix thoroughly by proportioning, when executing water to water content 70-80%, with 55-60 ℃ of diaphragm seal fermentation, kept 7-10 days.Use the substrate culture fruit and vegetable of above-mentioned prescription, average individual plant substrates quantity is the 10-15 liter.This matrix greatly reduces peat composed of rotten mosses consumption take rice husk and the discarded cotton seed hulls behind edible fungus culturing as main component, and it is with low cost easily to draw materials, and can be used as fertilizer after the use and apply the land for growing field crops, rich soil, free from environmental pollution.But the organic substrate type soilless culture substrate of this patent document does not have disease-resistant function.
CN200810139332.2 discloses a kind of method for cultivating organic matrix of vegetable that overcomes the facility meloidogyne.The cultivation bed of this kind cultivating method be not with the open soil box of soil isolation, alternative three cultivation medium formulas of this kind cultivating method are by its volume ratio prescription 1: rice husk: fermentation poultry dung: river sand=2: 1: 1; Prescription 2: decomposed cow dung: rice husk: river sand=5: 4: 1; Prescription 3: corn or wheat stalk fermentation materials: Chicken dung (or sheep excrement): river sand=4: 1: 1.After having dug soil box by specification, cultivation matrix is inserted in the groove, can be in cultivation bed the vegetables such as field planting tomato, watermelon.Meloidogyne can not invaded in the cultivation matrix that is distributed with the main root system of vegetables, and therefore, this kind method can reduce the facility meloidogyne to the harm of vegetables.What prevent and treat that root knot nematode disease adopts is engineering method but this patent document is mentioned, but not biological method.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of special-purpose organic culture substrate of capsicum of preventing and treating disease based on biological process and preparation method thereof.
It is composite that the present invention selects different organic wastes to carry out, make the nutrient of its compound meet the Absorption By Hot Pepper Plant requirement as far as possible, sterilize behind the fermentation maturity, add Trichoderma microbial inoculum and gemma bacillus agent with control Fusarium solani, epidemic disease, damping-off and root knot nematode disease function, namely get cultivation matrix of the present invention behind the composting certain hour.The a large amount of active Trichoderma, genus bacillus and the meta-bolites thereof that contain in the matrix have obvious restraining effect to pepper diseases.Through overtesting, obtained good effect.
The term explanation:
Biocontrol microorganisms: be that a class can be brought out it and produced resistance or secretion antibacterial substance in host plant, affect the breeding, growth of pathogenic bacteria until death; Or by antibiosis, Competition, predation etc., reduce the infection rate of pathogenic bacteria and pathogenic microorganism.
Technical scheme of the present invention is as follows:
Prevent and treat the special-purpose organic culture substrate of capsicum of disease, raw materials quality part is as follows:
Described Trichoderma microbial inoculum is the liquid Trichoderma bacterium liquid that Trichoderma is cultivated, ferments and prepare by prior art, and the effective bacterial content of bacterium liquid is 2 * 10
8Individual/gram～5 * 10
8Individual/gram.
Described Trichoderma bacterial classification is selected from trichoderma harziarum (T.harzianum) or viride (T.viride), wherein trichoderma harziarum ACCC30371, trichoderma harziarum ACCC30906, viride ACCC31911 or the viride ACCC30902 of preferred Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China english abbreviation ACCC).
Described gemma bacillus agent is the liquid genus bacillus bacterium liquid that genus bacillus is cultivated, ferments and prepare by prior art, and the effective bacterial content of bacterium liquid is 2 * 10
9Individual/gram～4 * 10
9Individual/gram.
Described genus bacillus bacterial classification is selected from subtilis (Bacillus subtilis), Bacillus sphaericus (Bacillus sphaericus) or bacillus pumilus (Bacillus pumilus); A kind of among subtilis CGMCC1.933, the subtilis CGMCC1.936 of preferred Chinese common micro-organisms culture presevation administrative center (China General Microbiological Culture Colletion Center english abbreviation CGMCC), Bacillus sphaericus CGMCC1.929, Bacillus sphaericus CGMCC1.930, bacillus pumilus CGMCC1.937, the bacillus pumilus CGMCC1.938 wherein.
Above-mentioned Trichoderma microbial inoculum and gemma bacillus agent belong to biocontrol fungicide.
Described furfural dregs is that corn cob is that main raw material is made the waste residue in the furfural process.
Described edible fungi residue is planting material discarded in the Edible Fungi, and main component is cotton seed hulls, crop straw, sawdust, edible fungus mycelium etc.Preferred mushroom cultivation waste material, the base-material main component is cotton seed hulls and corn cob.
The described dregs of fat are that the plant oil pressing factory utilizes squeezing process that raw material is pushed, comes unstuck and produce remaining residue behind the edible oil, comprise soya-bean oil slag, the peanut dregs of fat, the cottonseed dregs of fat or the Semen Brassicae campestris dregs of fat, can select one of them, also use capable of being combined.
Described wood chip be in the wood working process waste wood crushed material.Preferably, hardwood sawdust is crossed aperture 3.0mm sieve, places more than half a year under the physical environment.
Described microbial fermentation microbial inoculum is EM composite fungus agent (EM is the abbreviation of EffectiveMicroorganesms), and it is that 5 sections 10 such as photosynthetic bacteria group, yeast flora, lactobacillus, genus bacillus group, mycorhiza flora are belonged to 80 multiple-microorganisms compound a kind of typing microbial inoculum of cultivating in same state.Be brown translucent liquid, the pH value is between 3.5～4.5.Market is on sale.
According to the present invention, preferred, the special-purpose organic culture substrate raw materials quality of the capsicum part of preventing and treating disease is as follows:
Among the preparation method of the special-purpose organic culture substrate of the capsicum that prevents and treats disease of the present invention, relevant Trichoderma microbial inoculum and gemma bacillus agent are cultivated, are fermented by prior art, the invention provides following concrete operations condition, but are not limited in this.
The preparation method who prevents and treats the special-purpose organic culture substrate of capsicum of disease of the present invention, step is as follows:
(1) preparation of Trichoderma microbial inoculum:
To remove the peel potato 200 grams, add water 1000 grams, boil 20 minutes, filtered through gauze, filtrate 1000 grams that keep the skin wet add glucose 20 gram again, agar 16 grams, peptone 2 grams, potassium primary phosphate 2 grams, magnesium sulfate heptahydrate 1 gram is heated to all the components and dissolves, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, make the test tube slant, access Trichoderma bacterial classification is placed in 28 ℃ of constant incubators and cultivated 3 days after the cooling.After cultivate finishing routinely inoculum size and method be forwarded in the liquid nutrient medium, 28 ℃, 80 back and forth/minute shaking culture 4 days, get seed liquor.With in the inoculum size access liquid nutrient medium of seed liquor with 5～10wt%, in fermentor tank, cultivated 6 days under 28 ℃ of conditions again, get the Trichoderma microbial inoculum; The effective bacterial content of bacterium liquid is 2 * 10
8Individual/gram～5 * 10
8Individual/gram.
Described liquid nutrient medium: peeling potato 200 grams, add water 1000 grams, boiled 20 minutes, filtered through gauze, filtrate keep the skin wet 1000 the gram, add again glucose 20 grams, peptone 2 grams, potassium primary phosphate 2 grams, magnesium sulfate heptahydrate 1 gram, be heated to all the components and dissolve, sterilization is 30 minutes under 0.11 MPa pressure.
(2) preparation of gemma bacillus agent:
Inclined-plane seed: adopt the PDA substratum, will remove the peel potato 200 grams, add water 1000 grams, boiled 20 minutes, filtered through gauze, filtrate keep the skin wet 1000 the gram, add again glucose 20 grams, agar 18 grams are heated to all the components and dissolve, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, make the test tube slant, access genus bacillus bacterial classification is placed in 30 ℃ of constant incubators and cultivated 2 days after the cooling.Get the inclined-plane seed.
Liquid culture based formulas: analysis for soybean powder 5 grams, yeast extract paste 4 grams, peptone 4 grams, glucose 3 grams, Zulkovsky starch 5 grams, saltpetre 1 gram, calcium carbonate 0.5 gram, sodium-chlor 0.5 gram, potassium primary phosphate 0.5 gram, magnesium sulfate heptahydrate 0.5 gram, ferrous sulfate 0.01 gram, water 1000 grams, pH7.3.Sterilization is 30 minutes under 0.11 MPa pressure.Inoculum size and method are received the inclined-plane seed in the liquid nutrient medium routinely, 30 ℃, 80 back and forth/minute shaking culture 3 days, get seed liquor.With in the inoculum size access liquid nutrient medium of seed liquor with 5～8wt%, in fermentor tank, cultivated 4 days under 30 ℃ of conditions again, get gemma bacillus agent; The effective bacterial content of bacterium liquid is 2 * 10
9Individual/gram～4 * 10
9Individual/gram.
(3) by proportioning, get furfural dregs, edible fungi residue, the dregs of fat, wood chip, the microbial fermentation microbial inoculum mixes.The moisture of adjusting compound is 55～60wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5～7 days.Turning, temperature rise to 50 ℃ of timing again, keep 4～7 days.So operation repeats 2 times again, ferments altogether 27～42 days.Get the organic matter fermentation material.Gained organic matter fermentation material is carried out steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps the organic matter fermentation material of must sterilizing 7～10 hours.
(4) by proportioning, get the Trichoderma microbial inoculum of step (1) preparation, the gemma bacillus agent of step (2) preparation adds in the sterilization organic matter fermentation material of step (3) preparation, mix, the moisture of adjusting compound is 55～60wt%, composting is 4～6 days under 28 ℃～30 ℃ conditions, dry or 35 ℃～40 ℃ dry to moisture content 27～30wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
The application method of preventing and treating the special-purpose organic culture substrate of capsicum of disease of the present invention:
With this cultivation matrix cultivating container (basin or geosyncline etc.) of packing into, thickness is 15～25 centimetres, sows according to a conventional method pepper seed or the Hot Pepper Seedling of planting, and need not to use chemical pesticide, and other control measures are pressed the common cultivation matrix technology and got final product.
Excellent results of the present invention is as follows:
1. minimizing disease
Capsicum is one of vegetable variety than susceptible disease, is particularly generally occured in China various places by fungus-caused disease, and the easier morbidity of cultivation has a strong impact on yield of hot pepper and quality under facility condition.The capsicum fungal disease can be propagated by modes such as seed, seedling replantings, so adopt substrate culture also to be difficult to avoid disease to occur.The present invention is added in anti-bacteria in the cultivation matrix, has played prophylactic effect, has reduced pepper diseases.
2. protection of the environment
Chemical pesticide also has toxic except germ is played the killing action to other biology.According to the study, effective utilization of chemical pesticide only has about 10%, and major part remains in the environment, and long-term a large amount of the use can threatening environment safety.The present invention prevents and treats that Trichoderma and genus bacillus have good antagonistic activity to the capsicum pathogenic bacteria in the special-purpose organic culture substrate of capsicum of disease, to other Biosafety.
3. physical behavior is good
Because capsicum is longer breeding time, common organic culture substrate can continue to rot in the course of cultivation, has added wood chip in the matrix of the present invention, and its decomposition difficulty is large, can in the chili growth process, keep good physical behavior of long period, for chili growth provides good root system environment.
Embodiment
The present invention will be further described below in conjunction with embodiment, but be not limited to this.Raw material consumption umber among the embodiment is mass parts.Raw material is described as follows:
The Trichoderma bacterial classification is: trichoderma harziarum ACCC30906 and viride ACCC30902, and embodiment 1-2 ACCC30906 wherein, embodiment 3-4 ACCC30902, Chinese agriculture microbial strains preservation administrative center buys.
The genus bacillus bacterial classification is: subtilis CGMCC1.936 and Bacillus sphaericus CGMCC1.929, and embodiment 1-2 CGMCC1.936 wherein, embodiment 3-4 CGMCC1.929, Chinese common micro-organisms culture presevation administrative center buys.
Furfural dregs, Henan Hongye Chemical Industry Co., Ltd makes the waste residue in the furfural process take corn cob as main raw material.
The dregs of fat are that Shandong wide life limited-liability company produces.The embodiment 1-2 soya-bean oil dregs of fat, the embodiment 3-4 Semen Brassicae campestris dregs of fat.
The microbial fermentation microbial inoculum is the EM composite fungus agent, and Jiangxi Tianyi Biologic Technology Development Co., Ltd. produces.
Edible fungi residue, the mushroom cultivation waste material, the base-material main component is cotton seed hulls.
Wood chip is the poplar-branch crushed material, crosses aperture 3.0mm sieve, places more than half a year under the physical environment.
Embodiment 1:
The special-purpose organic culture substrate of capsicum of preventing and treating disease is composed as follows:
(1) preparation of Trichoderma microbial inoculum:
To remove the peel potato 200 grams, add water 1000 grams, boil 20 minutes, filtered through gauze, filtrate 1000 grams that keep the skin wet add glucose 20 gram again, agar 16 grams, peptone 2 grams, potassium primary phosphate 2 grams, magnesium sulfate heptahydrate 1 gram is heated to all the components and dissolves, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, make the test tube slant, access Trichoderma bacterial classification is placed in 28 ℃ of constant incubators and cultivated 3 days after the cooling.After cultivate finishing routinely inoculum size be forwarded to (it is identical with slant medium fill a prescription, does not just add agar) in the liquid nutrient medium with method, 28 ℃, 80 reciprocal/minute shaking culture 4 days get seed liquor.With in the inoculum size access liquid nutrient medium of seed liquor with 8wt%, in fermentor tank, cultivated 6 days under 28 ℃ of conditions again, get the Trichoderma microbial inoculum.The effective bacterial content of this bacterium liquid is 3.47 * 10
8Individual/gram.
(2) preparation of gemma bacillus agent:
The inclined-plane seed: adopt the PDA substratum, concrete operations are to remove the peel potato 200 grams, add water 1000 grams, boiled filtered through gauze 20 minutes, filtrate 1000 grams that keep the skin wet add glucose 20 gram, agar 18 grams again, being heated to all the components dissolves, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, makes the test tube slant, access genus bacillus bacterial classification is placed in 30 ℃ of constant incubators and cultivated 2 days after the cooling.
Fermented liquid: liquid culture based formulas: analysis for soybean powder 5 grams, yeast extract paste 4 grams, peptone 4 grams, glucose 3 grams, Zulkovsky starch 5 grams, saltpetre 1 gram, calcium carbonate 0.5 gram, sodium-chlor 0.5 gram, potassium primary phosphate 0.5 gram, magnesium sulfate heptahydrate 0.5 gram, ferrous sulfate 0.01 gram, water 1000 grams, pH7.3.Sterilization is 30 minutes under 0.11 MPa pressure.Inoculum size and method are received the inclined-plane seed in the liquid nutrient medium routinely, 30 ℃, 80 back and forth/minute shaking culture 3 days, get seed liquor.With in the inoculum size access liquid nutrient medium of seed liquor with 7wt%, in fermentor tank, cultivated 4 days under 30 ℃ of conditions again, get gemma bacillus agent.The effective bacterial content of this bacterium liquid is 2.91 * 10
9Individual/gram.
(3) get furfural dregs 310kg, edible fungi residue 240kg, dregs of fat 157kg, wood chip 280kg, microbial fermentation microbial inoculum 2kg mixes.The moisture of adjusting compound is 58wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 6 days.Turning, temperature rise to 50 ℃ of timing again, keep 6 days.So operation repeats 2 times again, ferments altogether 36 days.Get the organic matter fermentation material.The organic matter fermentation material is put into container, pass into steam, timing when temperature rises to 100 ℃ kept the organic matter fermentation material of must sterilizing 10 hours.
(4) getting the Trichoderma microbial inoculum 5kg of step (1) preparation, the gemma bacillus agent 6kg of step (2) preparation adds in the sterilization organic matter fermentation material of step (3) preparation, mix, the moisture of adjusting compound is 55wt%, composting is 6 days under 28 ℃ of conditions, dry in the air to moisture content 28wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
Embodiment 2:
The special-purpose organic culture substrate raw material of capsicum of preventing and treating disease is composed as follows:
(1) preparation of Trichoderma microbial inoculum and gemma bacillus agent is with embodiment 1.
(2) get furfural dregs 280kg, edible fungi residue 280kg, dregs of fat 176kg, wood chip 250kg, microbial fermentation microbial inoculum 1kg mixes.The moisture of adjusting compound is 60wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 7 days.Turning, temperature rise to 50 ℃ of timing again, keep 4 days.So operation repeats 2 times again, ferments altogether 33 days.Get the organic matter fermentation material.The organic matter fermentation material is put into container, pass into steam, timing when temperature rises to 100 ℃ kept the organic matter fermentation material of must sterilizing 8 hours.
(3) the Trichoderma microbial inoculum 6kg, the gemma bacillus agent 7kg that get step (1) preparation add in the sterilization organic matter fermentation material of step (2) preparation, mix, the moisture of adjusting compound is 60wt%, composting is 4 days under 30 ℃ of conditions, dry in the air to moisture content 30wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
Embodiment 3:
The special-purpose organic culture substrate raw material of capsicum of preventing and treating disease is composed as follows:
The preparation method:
(1) preparation of Trichoderma microbial inoculum and gemma bacillus agent is with embodiment 1, and difference is that the Trichoderma kind adopts ACCC30902, and the effective bacterial content of Trichoderma microbial inoculum bacterium liquid is 3.67 * 10
8Individual/gram; Genus bacillus is adopted CGMCC1.929, and the effective bacterial content of gemma bacillus agent bacterium liquid is 2.56 * 10
9Individual/gram.
(2) get furfural dregs 350kg, edible fungi residue 300kg, dregs of fat 134kg, wood chip 200kg, microbial fermentation microbial inoculum 3kg mixes.The moisture of adjusting compound is 55wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5 days.Turning, temperature rise to 50 ℃ of timing again, keep 5 days.So operation repeats 2 times again, ferments altogether 30 days.Get the organic matter fermentation material.The organic matter fermentation material is put into container, pass into steam, timing when temperature rises to 100 ℃ kept the organic matter fermentation material of must sterilizing 9 hours.
(3) the Trichoderma microbial inoculum 8kg, the gemma bacillus agent 5kg that get step (1) preparation add in the sterilization organic matter fermentation material of step (2) preparation, mix, the moisture of adjusting compound is 57wt%, composting is 5 days under 29 ℃ of conditions, dry in the air to moisture content 27wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
Embodiment 4:
The special-purpose organic culture substrate raw material of capsicum of preventing and treating disease is composed as follows:
The preparation method:
(1) preparation of Trichoderma microbial inoculum and gemma bacillus agent is with embodiment 1, and difference is that the Trichoderma kind adopts ACCC30902, and the effective bacterial content of Trichoderma microbial inoculum bacterium liquid is 3.67 * 10
8Individual/gram; Genus bacillus is adopted CGMCC1.929, and the effective bacterial content of gemma bacillus agent bacterium liquid is 2.56 * 10
9Individual/gram.
(2) get furfural dregs 250kg, edible fungi residue 300kg, dregs of fat 200kg, wood chip 237kg, microbial fermentation microbial inoculum 2kg mixes.The moisture of adjusting compound is 57wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 7 days.Turning, temperature rise to 50 ℃ of timing again, keep 7 days.So operation repeats 2 times again, ferments altogether 42 days.Get the organic matter fermentation material.The organic matter fermentation material is put into container, pass into steam, timing when temperature rises to 100 ℃ kept the organic matter fermentation material of must sterilizing 7 hours.
(3) the Trichoderma microbial inoculum 7kg, the gemma bacillus agent 4kg that get step (1) preparation add in the sterilization organic matter fermentation material of step (2) preparation, mix, the moisture of adjusting compound is 58wt%, composting is 4 days under 29 ℃ of conditions, dry in the air to moisture content 29wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
Comparative Examples: the coventional type cultivation matrix, Shandong Guangda Fertilizer Technology Co., Ltd. produces, and mainly is that organism and the vermiculite that becomes thoroughly decomposed makes with mass ratio mixing in 2: 1.Organic matter raw material is that Klinit dregs and furfural dregs are with the composition of 1: 1 mass ratio.
The effect of above embodiment 1～4 and Comparative Examples is as follows:
Test site: the county that conquers east, Shandong Province, Shandong Guangda Fertilizer Technology Co., Ltd. test farm.
Test period: year May in August, 2011 to 2012.
For trying capsicum variety: Shouguang goat's horn is yellow.
The cultivation of employing geosyncline, 25 centimetres of groove depths, long 800 centimetres, wide 40 centimetres, every groove is planted 2 row, and the interval is 60 centimetres between two grooves.If embodiment 1-4 and Comparative Examples be totally 5 processing, each processes plantation 2 grooves, triplicate.The cultivation matrix of embodiment 1-4 and Comparative Examples is placed in the cultivation bed, and cultivation matrix thickness is 20 centimetres, the Hot Pepper Seedling of planting according to a conventional method, and various management are carried out routinely.Effect sees Table 1.
Table 1. is compared the effect of embodiment 1-4 with Comparative Examples
| Cultivation matrix of the present invention | Output increases % | Sickness rate reduces % |
| Embodiment 1 | 11.3 | 60.1 |
| Embodiment 2 | 9.6 | 51.2 |
| Embodiment 3 | 10.5 | 57.0 |
| Embodiment 4 | 8.4 | 49.3 |
Claims (4)
1. prevent and treat the preparation method of the special-purpose organic culture substrate of capsicum of disease, raw materials quality part is as follows:
Described Trichoderma microbial inoculum is the liquid Trichoderma bacterium liquid that Trichoderma is cultivated, ferments and prepare by prior art, and the effective bacterial content of bacterium liquid is 2 * 10
8Individual/gram～5 * 10
8Individual/gram; Described Trichoderma bacterial classification is selected from trichoderma harziarum (T.harzianum) or viride (T.viride);
Described gemma bacillus agent is the liquid genus bacillus bacterium liquid that genus bacillus is cultivated, ferments and prepare by prior art, and the effective bacterial content of bacterium liquid is 2 * 10
9Individual/gram～4 * 10
9Individual/gram; Described genus bacillus bacterial classification is selected from subtilis (Bacillus subtilis), Bacillus sphaericus (Bacillus sphaericus) or bacillus pumilus (Bacillus pumilus);
Described microbial fermentation microbial inoculum is the EM composite fungus agent;
Step is as follows:
(1) preparation of Trichoderma microbial inoculum:
To remove the peel potato 200 grams, add water 1000 grams, boil 20 minutes, filtered through gauze, filtrate 1000 grams that keep the skin wet add glucose 20 gram again, agar 16 grams, peptone 2 grams, potassium primary phosphate 2 grams, magnesium sulfate heptahydrate 1 gram is heated to all the components and dissolves, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, make the test tube slant, access Trichoderma bacterial classification is placed in 28 ℃ of constant incubators and cultivated 3 days after the cooling; After cultivate finishing routinely inoculum size and method be forwarded in the liquid nutrient medium, 28 ℃, 80 back and forth/minute shaking culture 4 days, get seed liquor; With in the inoculum size access liquid nutrient medium of seed liquor with 5～10wt%, in fermentor tank, cultivated 6 days under 28 ℃ of conditions again, get the Trichoderma microbial inoculum; The effective bacterial content of bacterium liquid is 2 * 10
8Individual/gram～5 * 10
8Individual/gram;
Described liquid nutrient medium: peeling potato 200 grams, add water 1000 grams, boiled 20 minutes, filtered through gauze, filtrate keep the skin wet 1000 the gram, add again glucose 20 grams, peptone 2 grams, potassium primary phosphate 2 grams, magnesium sulfate heptahydrate 1 gram, be heated to all the components and dissolve, sterilization is 30 minutes under 0.11 MPa pressure;
(2) preparation of gemma bacillus agent:
Inclined-plane seed: adopt the PDA substratum, will remove the peel potato 200 grams, add water 1000 grams, boiled 20 minutes, filtered through gauze, filtrate keep the skin wet 1000 the gram, add again glucose 20 grams, agar 18 grams are heated to all the components and dissolve, the packing test tube, sterilization is 30 minutes under 0.11 MPa pressure, make the test tube slant, access genus bacillus bacterial classification is placed in 30 ℃ of constant incubators and cultivated 2 days after the cooling; Get the inclined-plane seed;
Liquid culture based formulas: analysis for soybean powder 5 grams, yeast extract paste 4 grams, peptone 4 grams, glucose 3 grams, Zulkovsky starch 5 grams, saltpetre 1 gram, calcium carbonate 0.5 gram, sodium-chlor 0.5 gram, potassium primary phosphate 0.5 gram, magnesium sulfate heptahydrate 0.5 gram, ferrous sulfate 0.01 gram, water 1000 grams, pH7.3; Sterilization is 30 minutes under 0.11 MPa pressure; Inoculum size and method are received the inclined-plane seed in the liquid nutrient medium routinely, 30 ℃, 80 back and forth/minute shaking culture 3 days, get seed liquor; With in the inoculum size access liquid nutrient medium of seed liquor with 5～8wt%, in fermentor tank, cultivated 4 days under 30 ℃ of conditions again, get gemma bacillus agent; The effective bacterial content of bacterium liquid is 2 * 10
9Individual/gram～4 * 10
9Individual/gram;
(3) by proportioning, get furfural dregs, edible fungi residue, the dregs of fat, wood chip, the microbial fermentation microbial inoculum mixes; The moisture of adjusting compound is 55～60wt%, and fermentation reactor system becomes thoroughly decomposed; Temperature rises to 55 ℃ of timing, keeps 5～7 days; Turning, temperature rise to 50 ℃ of timing again, keep 4～7 days; So operation repeats 2 times again, ferments altogether 27～42 days, gets the organic matter fermentation material; Gained organic matter fermentation material is carried out steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps the organic matter fermentation material of must sterilizing 7～10 hours;
(4) by proportioning, get the Trichoderma microbial inoculum of step (1) preparation, the gemma bacillus agent of step (2) preparation adds in the sterilization organic matter fermentation material of step (3) preparation, mix, the moisture of adjusting compound is 55～60wt%, composting is 4～6 days under 28 ℃～30 ℃ conditions, dry or 35 ℃～40 ℃ dry to moisture content 27～30wt%, must prevent and treat the special-purpose organic culture substrate of capsicum of disease.
2. the preparation method of the special-purpose organic culture substrate of the capsicum that prevents and treats disease as claimed in claim 1, its feature is as follows raw materials quality part:
3. the preparation method who prevents and treats the special-purpose organic culture substrate of capsicum of disease as claimed in claim 1 or 2 is characterized in that described Trichoderma bacterial classification is selected from trichoderma harziarum ACCC30371, trichoderma harziarum ACCC30906, viride ACCC31911 or viride ACCC30902.
4. the preparation method of the special-purpose organic culture substrate of the capsicum that prevents and treats disease as claimed in claim 1 or 2 is characterized in that the genus bacillus bacterial classification is selected from a kind of among subtilis CGMCC1.933, subtilis CGMCC1.936, Bacillus sphaericus CGMCC1.929, Bacillus sphaericus CGMCC1.930, bacillus pumilus CGMCC1.937, the bacillus pumilus CGMCC1.938.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101988137A CN102718577B (en) | 2012-06-15 | 2012-06-15 | Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101988137A CN102718577B (en) | 2012-06-15 | 2012-06-15 | Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102718577A CN102718577A (en) | 2012-10-10 |
| CN102718577B true CN102718577B (en) | 2013-10-30 |
Family
ID=46944484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101988137A Expired - Fee Related CN102718577B (en) | 2012-06-15 | 2012-06-15 | Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102718577B (en) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934583B (en) * | 2012-11-30 | 2014-03-26 | 赖月娥 | Integrated pest and disease prevention and control management of hot peppers |
| CN103299811B (en) * | 2013-06-18 | 2014-07-23 | 江苏省农业科学院 | Method for enhancing soil waterflooding effect to control pepper phytophthora blight |
| CN104177138B (en) * | 2014-07-25 | 2017-02-15 | 山东宝源生物科技股份有限公司 | Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer |
| CN105801237A (en) * | 2014-12-31 | 2016-07-27 | 徐立江 | Capsicum soilless culture matrix for preventing and controlling insect diseases and preparation method thereof |
| CN104892088A (en) * | 2015-03-24 | 2015-09-09 | 上海宇强贸易有限公司 | Soil-free cultivation medium with microorganisms and promoting healthy growth and production of crops and preparation method thereof |
| CN105175106A (en) * | 2015-08-17 | 2015-12-23 | 中国科学院沈阳应用生态研究所 | Crop cultivation matrix and preparation method therefor |
| CN105145309A (en) * | 2015-09-23 | 2015-12-16 | 山东农业大学 | Integrated ecological strawberry cultivation method for reducing pest and disease damage and improving fruit quality |
| CN105684779A (en) * | 2016-01-26 | 2016-06-22 | 赵兰 | Compound substrate for hot-pepper plug seedling and preparation method thereof |
| CN105815144A (en) * | 2016-04-06 | 2016-08-03 | 牛福永 | Strong pepper seedling raising method |
| CN105815143A (en) * | 2016-04-06 | 2016-08-03 | 牛福永 | Seedling-raising matrix for cultivating strong pepper seedlings |
| CN105794535A (en) * | 2016-04-14 | 2016-07-27 | 梁英强 | Pepper seedling culture matrix |
| CN106045775A (en) * | 2016-05-31 | 2016-10-26 | 中国热带农业科学院环境与植物保护研究所 | Culture medium for overcoming pepper successive cropping obstacle and preparing method thereof |
| CN106478266A (en) * | 2016-10-21 | 2017-03-08 | 广州市林业和园林科学研究院 | A kind of disease preventing type cultivation matrix and its preparation method and application |
| CN106916012A (en) * | 2017-03-13 | 2017-07-04 | 安徽省卫国农业科技有限公司 | A kind of fertilizer for improving Quality of Capsicum |
| CN106810379A (en) * | 2017-03-14 | 2017-06-09 | 山东省联合农药工业有限公司 | A kind of pepper seedling raising medium and preparation method and the method that pepper seedling raising is carried out using the matrix |
| CN107306660A (en) * | 2017-08-24 | 2017-11-03 | 广西田阳煦日农业科技发展有限公司 | A kind of prevention black rotten method of succulent |
| CN107935667A (en) * | 2017-10-11 | 2018-04-20 | 湖北省农业科学院植保土肥研究所 | Solanaceous vegetable seedling raising ground substance fermentation materials and preparation method thereof, solanaceous vegetable seedling raising ground substance and preparation method thereof |
| CN109041845A (en) * | 2018-08-21 | 2018-12-21 | 杭州富阳飞博科技有限公司 | Zelkova schneideriana is taken root cutting medium and preparation method thereof |
| CN110122266A (en) * | 2018-11-22 | 2019-08-16 | 贵州恒创源农业发展有限公司 | A kind of dedicated organic culture substrate preventing capsicum pest and disease damage and its preparation process |
| CN110024659A (en) * | 2019-04-09 | 2019-07-19 | 福建农林大学 | A method of utilizing edible fungi residue production matrix and secondary use |
| CN110054517A (en) * | 2019-05-10 | 2019-07-26 | 潍坊市烟草专卖局 | A method of Trichoderma atroviride organic fertilizer is prepared using mushroom bran |
| CN113603544A (en) * | 2021-09-07 | 2021-11-05 | 山东新超农业科技有限公司 | Preparation method of pepper biological bacterial fertilizer |
| CN113698235A (en) * | 2021-09-07 | 2021-11-26 | 山东新超农业科技有限公司 | Preparation method of disease-resistant biological bacterial fertilizer for increasing yield of field crops |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101031203A (en) * | 2004-09-24 | 2007-09-05 | 生物耐克斯特公司 | Compositions for use against one or more pathogens |
| CN101734960A (en) * | 2009-12-18 | 2010-06-16 | 山东光大肥业科技有限公司 | Special organic fertilizer for leaf-vegetable organic vegetable and preparation method |
| CN101759493A (en) * | 2009-10-21 | 2010-06-30 | 山东光大肥业科技有限公司 | Biological disease-preventing organic fertilizer and preparation method thereof |
| CN101913961A (en) * | 2010-08-12 | 2010-12-15 | 新疆天枣源龟兹生物技术有限责任公司 | Weathered coal bio-organic fertilizer and production method thereof |
| CN102027998A (en) * | 2009-09-24 | 2011-04-27 | 华东理工大学 | Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof |
| CN102417424A (en) * | 2011-08-23 | 2012-04-18 | 山东光大肥业科技有限公司 | Special biological organic fertilizer for traditional Chinese medicinal material isatis and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000109386A (en) * | 1998-10-01 | 2000-04-18 | Asahi Kankyo System Kk | Composting method of organic waste and evaluation method of composted material thereby |
-
2012
- 2012-06-15 CN CN2012101988137A patent/CN102718577B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101031203A (en) * | 2004-09-24 | 2007-09-05 | 生物耐克斯特公司 | Compositions for use against one or more pathogens |
| CN102027998A (en) * | 2009-09-24 | 2011-04-27 | 华东理工大学 | Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof |
| CN101759493A (en) * | 2009-10-21 | 2010-06-30 | 山东光大肥业科技有限公司 | Biological disease-preventing organic fertilizer and preparation method thereof |
| CN101734960A (en) * | 2009-12-18 | 2010-06-16 | 山东光大肥业科技有限公司 | Special organic fertilizer for leaf-vegetable organic vegetable and preparation method |
| CN101913961A (en) * | 2010-08-12 | 2010-12-15 | 新疆天枣源龟兹生物技术有限责任公司 | Weathered coal bio-organic fertilizer and production method thereof |
| CN102417424A (en) * | 2011-08-23 | 2012-04-18 | 山东光大肥业科技有限公司 | Special biological organic fertilizer for traditional Chinese medicinal material isatis and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2000-109386A 2000.04.18 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102718577A (en) | 2012-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102718577B (en) | Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate | |
| CN101696390B (en) | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof | |
| CN102964178B (en) | Composite microbial bactericide, and preparation method and application thereof | |
| CN101659933B (en) | Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof | |
| CN101759494B (en) | Diseases-prevention organic fertilizer prepared by edible fungi residue | |
| KR101016859B1 (en) | Antagonistic bacteria and its microbial organic fertilizers to prevent the wilting of serial bananas | |
| CN104496707B (en) | Fertilizer and preparation method and application is controlled a kind of liquid compound bio more | |
| CN102249753B (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN102643148B (en) | Special biological disease-resistant vermin-proof culture medium for flowers and preparation method thereof | |
| CN102154156B (en) | Antagonists capable of preventing and controlling tomato bacterial wilt or knot nematode and microbial organic fertilizer thereof | |
| CN102701803B (en) | Special cultivation substrate for fruit type organic vegetables and preparation method of special cultivation substrate | |
| WO2011032330A1 (en) | Antagonistic bacteria for preventing and treating bacterial wilt disease of continuously planted tobacco and microorganism organic fertilizer thereof | |
| CN102690769A (en) | Compound microbial bacterial preparation for efficiently degrading feces and preparation method and application of compound microbial bacterial preparation | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN102627504B (en) | Culture substrate special for organic herbal fruits and preparation method thereof | |
| CN103074287B (en) | The antibacterial bacillus amyloliquefaciens of a kind of growth-promoting, amendment preparation method and application | |
| CN104761308A (en) | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer | |
| CN104016745B (en) | microbial organic fertilizer and preparation method thereof | |
| CN105948831B (en) | A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains | |
| CN103651582A (en) | Broad-spectrum composite microbial fungicide and preparation method thereof | |
| CN102972192A (en) | Method for improving pathogen-inhibiting and disease-controlling pesticide effect of rapeseed meal by applying myrosinase preparation in field | |
| CN102443559B (en) | Baclillus subtilis used for controlling cotton verticillium wilt and application thereof | |
| CN103951483A (en) | Biological antibacterial fertilizer for preventing and controlling soil-borne diseases of crops as well as production process and application of biological antibacterial fertilizer | |
| CN103275892B (en) | Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes | |
| CN105967900A (en) | Pest-resistant nutrient solution for indoors cultivated flowers and plants and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131030 Termination date: 20140615 |
|
| EXPY | Termination of patent right or utility model |