CN104761308A - Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer - Google Patents
Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer Download PDFInfo
- Publication number
- CN104761308A CN104761308A CN201410768533.4A CN201410768533A CN104761308A CN 104761308 A CN104761308 A CN 104761308A CN 201410768533 A CN201410768533 A CN 201410768533A CN 104761308 A CN104761308 A CN 104761308A
- Authority
- CN
- China
- Prior art keywords
- paecilomyces lilacinus
- preparation
- nursing
- microbial
- bacterial fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg/mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.
Description
Technical field
The present invention relates to microbial-bacterial fertilizer technical field, particularly a kind of Synthesis and applications of Synthesis and applications of soil ecology nursing microbial-bacterial fertilizer.
Background technology
Nemas is the class root disease that coelenterates nematode causes, and has a strong impact on crop yield, as root knot nematode (Meloidogyne) main harm vegetables root, side root and fibrous root the most vulnerable.Root of being injured produces the knob or root knot that differ in size, and root knot is just white, soft, after become light tan or Vandyke brown, surface irregularity, sometimes split.Cutting root knot open, can see white pearl small-particle, is the female adult worm of root knot nematode.Vegetables grow after infecting by root knot nematode slowly, and blade is little and yellow.When morbidity is heavy, plant is short and small, shaky or abnormal seeding, and when meeting high temperature and drought weather, plant is easily wilted even withered.
Since spread along with the increase, particularly heliogreenhouse of vegetable at protected field production area, multiple crop index increases, and continuous cropping is serious in addition, and nemas occurs day by day serious, and nearly all crop has eelworm harm.One of parasitic nematode important pathogen thing becoming plant infectious diseases, has a significant impact whole world agriculture production.Nematode generally can cause the underproduction 10% ~ 20%, seriously can reach 30% ~ 40%, even have no harvest.According to estimates, the loss that the whole world causes to grain and fibre crops because of eelworm harm is every year approximately 12.3%, causes the loss of about 1,000 hundred million dollars.China's Agricultural planting is every year because of nearly 23,300,000,000 yuan of damage losses that nematode is caused.
In the control of current nematode most widely used general be adopt nematocides (as Furadan etc.) to carry out chemical prevention, but its, contaminate environment, spoiled soil biota poisonous to people and animals, and prevention effect is not very good.Increasing scholar recognizes that controlling parasitic nematode must take aggregate measures or adopt the Sustainable Treatment system being core with biological and ecological control.Fungi studies more natural enemy biology in biocontrol of nematodes, is also one of of paramount importance bio-control factors.
Wherein the most famous, be also a most important fungi be that Paecilomyces lilacinus (Paecilomyces lilacinus (Thom) Samson) is distributed widely in all over the world, easily from soil, fungi sclerotium, nematode obtains with insect is separated, and easily cultivates.Paecilomyces lilacinus Be very effective, and there is long-lasting; Preventing and treating usefulness to multiple nematode, is the most promising biological prevention and control agent of control parasitic nematode.
Chinese scholars has carried out large quantifier elimination to Paecilomyces lilacinus, facts have proved for many years: Paecilomyces lilacinus Be very effective, and has long-lasting; Preventing and treating usefulness to multiple nematode, is the most promising biological prevention and control agent of control parasitic nematode.
Simultaneously, in the process of growth of Paecilomyces lilacinus, particularly in Submerged fermentation process, this bacterium can produce abundant derivative, the similar indolylacetic acid product of the first, its the most significant physiological function is the growth promoting root system of plant and plant when lower concentration, can also produce the effect of similar growth hormone and phytokinin in addition.Therefore use Paecilomyces lilacinus at root system and obviously can not only suppress nematode infection, and the growth of plant nutrition organ can be promoted, also have promoter action to the Germination and growth of seed simultaneously.
Paecilomyces lilacinus also has antagonistic activity to various plants pathogenic bacteria, there are some researches show: Paecilomyces lilacinus bacterial strain 36-1 has remarkable antagonistic activity to 8 kind of plant pathogenic bacterias, they are corn southern leaf blights, wheat scab, cotton wilt, water rice bakanae disease, rice sheath blight disease, citrus pathogens penicillium, citrus green mold bacterium, cucumber anthracnose, Abdul (2000) utilizes Paecilomyces lilacinus to control cotton bacterialo wilt disease and cotton virus disease, result shows that paecilomycerol can obviously reduce cotton (NIAB-18, CIM-240 kind) Disease, and effective anti-bacteria blight, from greenhouse, also filter out high antagonism power bacterial strain, be sprayed on wheat leaf blade, in order to control speckled leaf blotch.Correlative study also confirms: Paecilomyces lilacinus significantly can reduce the sickness rate of Sunflower Receptacle and natto root knot, and can suppress the tomato root rot that caused by Fusarium.
Paecilomyces lilacinus can also promote the dissolving of difficultly-soluble phosphates, through laboratory test: Paecilomyces lilacinus to the correction degradation rate of Volaton about 94.Similar research also shows, Paecilomyces varioti can also promote the decomposition of many chemical polymerization things (as organophosphorus pesticide, leather-making waste water etc.).
In sum, Paecilomyces lilacinus not only can develop the wireworm-killing biologic agricultural chemicals becoming outstanding, and can prevent and treat plurality of plant diseases simultaneously, also can be used as efficient bio-feritlizer and uses, and reduces the residual of chemical pesticide and chemical fertilizer.
Paecilomyces lilacinus is that the habit of a kind of soil and various plants rhizosphere occupies bacterium, the embryo in energy failure line worm's ovum and early larvae.Paecilomyces lilacinus contains leucinostatin and has eelworm-killing activity.Paecilomyces lilacinus to can at multiple nature culture, as grown in agricultural byproducts waste material, waste residue and plant leaf or plant vat liquor.Although manioc waste and mushroom slag are the fabulous culture mediumes of the beneficial microorganisms such as Paecilomyces lilacinus, but the domestic research to bacterium chaff recycling mainly concentrates on regeneration plantation edible mushrooms, directly as aspects such as organic fertilizer or organic commercial fertilizer raw materials for production, and it is less to be used for cultivating beneficial microorganism research.
Chinese invention patent " a kind of method of producing sterilant with brew-house's waste material " (application number 95118468.7) discloses a kind of sterilant preparation method containing Paecilomyces lilacinus, be seeded on fungi culture medium inclined-plane by the Paecilomyces lilacinus bacterial classification after purifying, inoculate by Fructus Hordei Germinatus groove after cultivating, in the nutrient solution that waste beer yeast and Fructus Hordei Germinatus groove pressed juice are made into, be adsorbed onto on the solid medium that Fructus Hordei Germinatus groove and waste yeast cream forms again through shaking culture, 5-15 days is cultivated at 15-30 DEG C, the Paecilomyces lilacinus microbial inoculum made is as a kind of sterilant, can be used for the control of nematode of many farm crop, effectively can reduce population density, improve crop yield.And manufacture this microbial inoculum with the waste material of brew-house, both can make waste resource recovery, reduce environmental pollution again.The essence of this patent is only to utilize brew-house's waste material to carry out the cultivation of purple paecilomycerol as bacterium base, does not relate to the nursing of comprehensive soil ecology.
It is predicted, coming 10 years nematode will be still one of the Major Diseases of farm crop.Therefore, when selecting the method for control root knot nematode, should not put undue emphasis on the effect of a certain method, also lacking a kind of comprehensive Prevention Technique at present.
Summary of the invention
The present invention, in order to solve the problem, provides a kind of soil ecology to nurse the Synthesis and applications of microbial-bacterial fertilizer.
The technical solution adopted in the present invention is:
A kind of Synthesis and applications of soil ecology nursing microbial-bacterial fertilizer, consist predominantly of machine matter content >=40%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=0.2 hundred million CFU/ gram, wherein Paecilomyces lilacinus number >=0.1 hundred million CFU/ gram.
As preferably, soil ecology nursing microbial-bacterial fertilizer, consists predominantly of machine matter content >=50%, comprises photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=200,000,000 CFU/ gram, wherein Paecilomyces lilacinus number >=0.2 hundred million CFU/ gram.
As preferentially, soil ecology nursing microbial-bacterial fertilizer, consists predominantly of machine matter content >=50%, comprises photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=500,000,000 CFU/ gram, wherein Paecilomyces lilacinus number >=200,000,000 CFU/ gram.
The present invention also provides this soil ecology to nurse the preparation method of microbial-bacterial fertilizer, comprise the following steps "
(1) preparation of Paecilomyces lilacinus liquid spawn: the test tube slant culture medium inoculated Paecilomyces lilacinus pure strain of the suitable Paecilomyces lilacinus growth of preparation, is cultured to and occurs lavender powdery conidium; Again to prepare the one-level test tube slant bacterial classification of the triangular flask liquid nutrient medium inoculation Paecilomyces lilacinus of suitable Paecilomyces lilacinus growth, constant temperature rotating and culturing is stand-by, and water used in above-mentioned culturing process is the high-energy water of molecular oscillation frequency at more than 3000Mhz;
(2) for the production of the enlarged culturing of liquid spawn: molecular oscillation frequency at the high-energy water of more than 3000Mhz as the large fermentation tank substratum of the suitable Paecilomyces lilacinus growth of water source preparation, ripe triangular flask Paecilomyces lilacinus liquid spawn is cultivated by by above-mentioned triangular flask liquid nutrient medium, by in aseptic technique access fermentor tank, continue to pass into sterile air and carry out stir culture;
(3) configuration of substratum: by biological organic matter material and organism decomposing agent according to 100: 0.3-0.5 ratio fully mix, and carry out fermenting, becoming thoroughly decomposed, this organism decomposing agent contains the bacterial classifications such as photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus;
(4) the Paecilomyces lilacinus microbial inoculum enlarged culturing stage is added: after the biological organism material temperature degree fermenting, become thoroughly decomposed is down to 40 DEG C, add the Paecilomyces lilacinus microbial inoculum cultivated in step (2), additional proportion is: biological organism material: Paecilomyces lilacinus microbial inoculum=1000: 0.5-5, Paecilomyces lilacinus microbial inoculum and biological organism material is fully mixed and ferments;
(5) Secondary Fermentation: the material in step (4) is carried out stacking Secondary Fermentation, leavening temperature is 30-35 DEG C, keeps biological organism material water rate control 30%, fermentation time: 3-5 days, completes product preparation.
As preferably, described in step (1), the solid slant culture method of Paecilomyces lilacinus liquid spawn is: configuration Cha Shi solid medium or PDA solid medium, sterilizing, streak inoculation Paecilomyces lilacinus, is placed in constant incubator to be cultured at 25-30 DEG C and occurs lavender powdery conidium; Liquid cultivating method be culture medium prescription with in described solid slope spawn culture based formulas except not adding agar all the other compositions or preparation method identical, on aseptic operating platform, hook from the Paecilomyces lilacinus that solid slant culture base is preserved with transfering loop and get conidium one ring, be seeded in triangular flask in liquid nutrient medium, cultivate 36 hours for shaking table 110 ~ 150rpm, 25-30 DEG C;
As preferably, culture condition described in step (2) is fermentor tank internal pressure is 0.03 MPa, and temperature is 25-28 DEG C, and incubation time 54 hours is stand-by.
As preferably, biological organic matter material described in step (3) is the mixture of one or more of fowl and animal excrement, agricultural crop straw, bacterium mushroom slag, manioc waste, and leavening temperature is 60-70 DEG C, fermentation time 5-7 days;
As preferably, continuing fermentation time described in step (4) is 24 hours, and leavening temperature is 30-35 DEG C;
As preferably, aforesaid method can also comprise granulation and wrapping process.
Described in described step (1), Cha Shi liquid culture based formulas is: NaNO3 3g, K2HPO4 1g, KCl 0.5g, MgSO47H2O 0.5g, FeSO40.01g, sucrose 30g, H2O 1000mL, pH nature; The preparation method of PDA solid medium is: take 200g potato, and clean peeling is cut into small pieces, and the 1000mL that adds water boils half an hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, then adds 10 ~ 20g glucose, filtered through gauze while hot after fully dissolving.
The present invention also provides this soil ecology to nurse the using method of microbial-bacterial fertilizer: using this soil ecology nursing microbial-bacterial fertilizer as during base manure is manured into soil before crop-planting, every mu of consumption is 100-200kg, can to soil particularly plastic shed soil play good result.Show and can improve soil microorganisms environment, effective reduction soil salinization, harden degree, increases soil fertility, and promotes plant growth, obviously can alleviate the harm of the nemases such as various crop root knot nematode, Cyst nematode, Ditylenchus dipsaci simultaneously, alleviate continuous cropping obstacle.
Beneficial effect of the present invention:
(1) this soil ecology nursing microbial-bacterial fertilizer contains multiple effective viable bacteria, and the toxic substance of Pesticide Residue in Soil, fertilizer residue can be degraded to non-toxic substance by its specific enzymes produced, and avoids the injury to crop; Phosphorus decomposing, potassium decomposing, nitrogen fixation, effectively can improve the utilization ratio of fertilizer.
(2) Paecilomyces lilacinus contained by, obviously can alleviate the harm of the nemases such as various crop root knot nematode, Cyst nematode, Ditylenchus dipsaci, alleviates continuous cropping obstacle.
(3) this soil ecology is nursed in the preparation process of microbial-bacterial fertilizer and is employed the high-energy water of molecular oscillation frequency at more than 3000Mhz, the energy water machine that this energy water is sold by market is prepared, the substratum of this high-energy water preparation biological bacteria, there is the effect starting and strengthen bacterial strain vigor fast, relative to common water medium, present method shortens incubation time, improves culture efficiency.
(4) this soil ecology nursing microbial bacteria fertilizer product use range is extensive, has good effect special in booth
Embodiment
Embodiment 1:
A kind of Synthesis and applications product of soil ecology nursing microbial-bacterial fertilizer: its component is organic content >=40%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=0.2 hundred million CFU/ gram, wherein Paecilomyces lilacinus number >=0.1 hundred million CFU/ gram.
The preparation method of this soil ecology nursing microbial bacteria fertilizer product:
(1) preparation of Paecilomyces lilacinus liquid spawn: water be the high-energy water configuration Cha Shi solid medium of molecular oscillation frequency at more than 3000Mhz, sterilizing, streak inoculation Paecilomyces lilacinus, is placed in constant incubator to be cultured at 25-30 DEG C and occurs lavender powdery conidium; Liquid cultivating method is that Cha Shi solid medium does not add agar, on aseptic operating platform, hook from the Paecilomyces lilacinus that solid slant culture base is preserved with transfering loop and get conidium one ring, be seeded in triangular flask in liquid nutrient medium, cultivate 36 hours for shaking table 110-150rpm, 25-30 DEG C;
(2) for the production of the enlarged culturing of liquid spawn: molecular oscillation frequency at the high-energy water of more than 3000Mhz as the large fermentation tank substratum of the suitable Paecilomyces lilacinus growth of water source preparation, ripe triangular flask Paecilomyces lilacinus liquid spawn is cultivated by by above-mentioned triangular flask liquid nutrient medium, by in aseptic technique access fermentor tank, continue to pass into sterile air and carry out stir culture, fermentor tank internal pressure is 0.03 MPa, temperature is 25-28 DEG C, and incubation time 54 hours is stand-by;
(3) configuration of substratum: by biological organic matter material and organism decomposing agent according to 100: 0.3-0.5 ratio fully mix, and ferment, wherein biological organic matter material is the feces of livestock and poultry 50 parts that becomes thoroughly decomposed; 50 parts, edible fungus culturing waste material; Organic matter decomposing inoculant 0.3 part;
(4) the Paecilomyces lilacinus microbial inoculum enlarged culturing stage is added: after the biological organism material temperature degree fermenting, become thoroughly decomposed is down to 40 DEG C, add the Paecilomyces lilacinus microbial inoculum cultivated in step (2), additional proportion is: biological organism material: Paecilomyces lilacinus microbial inoculum=1000; 0.5, Paecilomyces lilacinus microbial inoculum and biological organism material are fully mixed and ferments, leavening temperature is 60-70 DEG C, fermentation time 5-7 days;
(5) in the Secondary Fermentation stage: the material in step (4) is carried out stacking Secondary Fermentation, leavening temperature is 30-35 DEG C, keeps biological organism material water rate control 30%, fermentation time: 3-5 days, completes product preparation.
(6) granulation and wrapping process, after testing, the technical indicator of product meets the requirements.
The application of this soil ecology nursing microbial-bacterial fertilizer, apply in Plantula Brassicae chinensis planting greenhouse soil before crop-planting as base manure, every mu of consumption 200kg, eelworm harm obviously reduces, and product increases by 15%.
Embodiment 2:
A kind of Synthesis and applications product of soil ecology nursing microbial-bacterial fertilizer: its component is organic content >=50%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=200,000,000 CFU/ gram, wherein Paecilomyces lilacinus number >=0.2 hundred million CFU/ gram.
The preparation method of this soil ecology nursing microbial bacteria fertilizer product:
(1) preparation of Paecilomyces lilacinus liquid spawn: water be the high-energy water configuration Cha Shi solid medium of molecular oscillation frequency at more than 3000Mhz, sterilizing, streak inoculation Paecilomyces lilacinus, is placed in constant incubator to be cultured at 25-30 DEG C and occurs lavender powdery conidium; Liquid cultivating method is that Cha Shi solid medium does not add agar, on aseptic operating platform, hook from the Paecilomyces lilacinus that solid slant culture base is preserved with transfering loop and get conidium one ring, be seeded in triangular flask in liquid nutrient medium, cultivate 36 hours for shaking table 110-150rpm, 25-30 DEG C;
(2) for the production of the enlarged culturing of liquid spawn: molecular oscillation frequency at the high-energy water of more than 3000Mhz as the large fermentation tank substratum of the suitable Paecilomyces lilacinus growth of water source preparation, ripe triangular flask Paecilomyces lilacinus liquid spawn is cultivated by by above-mentioned triangular flask liquid nutrient medium, by in aseptic technique access fermentor tank, continue to pass into sterile air and carry out stir culture, fermentor tank internal pressure is 0.03 MPa, temperature is 25-28 DEG C, and incubation time 54 hours is stand-by;
(3) configuration of substratum: by biological organic matter material and organism decomposing agent according to 100: 0.3-0.5 ratio fully mix, and ferment, wherein biological organic matter material is the feces of livestock and poultry 50 parts that becomes thoroughly decomposed; 50 parts, edible fungus culturing waste material; Organic matter decomposing inoculant 0.3 part;
(4) the Paecilomyces lilacinus microbial inoculum enlarged culturing stage is added: after the biological organism material temperature degree fermenting, become thoroughly decomposed is down to 40 DEG C, add the Paecilomyces lilacinus microbial inoculum cultivated in step (2), additional proportion is: biological organism material: Paecilomyces lilacinus microbial inoculum=1000: 01, Paecilomyces lilacinus microbial inoculum and biological organism material are fully mixed ferment, leavening temperature is 60-70 DEG C, fermentation time 5-7 days;
(5) in the Secondary Fermentation stage: the material in step (4) is carried out stacking Secondary Fermentation, leavening temperature is 30-35 DEG C, keeps biological organism material water rate control 30%, fermentation time: 3-5 days, completes product preparation.
(6) granulation and wrapping process, after testing, the technical indicator of product meets the requirements.
The application of this soil ecology nursing microbial-bacterial fertilizer, apply in Plantula Brassicae chinensis planting greenhouse soil before crop-planting as base manure, every mu of consumption 150k g, eelworm harm obviously reduces, and product increases by 25%.
Embodiment 3:
A kind of Synthesis and applications product of soil ecology nursing microbial-bacterial fertilizer: its component is organic content >=50%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=500,000,000 CFU/ gram, wherein Paecilomyces lilacinus number 200,000,000 CFU/ gram.
The preparation method of this soil ecology nursing microbial bacteria fertilizer product:
(1) preparation of Paecilomyces lilacinus liquid spawn: water be the high-energy water configuration Cha Shi solid medium of molecular oscillation frequency at more than 3000Mhz, sterilizing, streak inoculation Paecilomyces lilacinus, is placed in constant incubator to be cultured at 25-30 DEG C and occurs lavender powdery conidium; Liquid cultivating method is that Cha Shi solid medium does not add agar, on aseptic operating platform, hook from the Paecilomyces lilacinus that solid slant culture base is preserved with transfering loop and get conidium one ring, be seeded in triangular flask in liquid nutrient medium, cultivate 36 hours for shaking table 110-150rpm, 25-30 DEG C;
(2) for the production of the enlarged culturing of liquid spawn: molecular oscillation frequency at the high-energy water of more than 3000Mhz as the large fermentation tank substratum of the suitable Paecilomyces lilacinus growth of water source preparation, ripe triangular flask Paecilomyces lilacinus liquid spawn is cultivated by by above-mentioned triangular flask liquid nutrient medium, by in aseptic technique access fermentor tank, continue to pass into sterile air and carry out stir culture, fermentor tank internal pressure is 0.03 MPa, temperature is 25-28 DEG C, and incubation time 54 hours is stand-by;
(3) configuration of substratum: by biological organic matter material and organism decomposing agent according to 100: 0.3-0.5 ratio fully mix, and ferment, wherein biological organic matter material is the feces of livestock and poultry 50 parts that becomes thoroughly decomposed; 50 parts, edible fungus culturing waste material; Organic matter decomposing inoculant 0.3 part;
(4) the Paecilomyces lilacinus microbial inoculum enlarged culturing stage is added: after the biological organism material temperature degree fermenting, become thoroughly decomposed is down to 40 DEG C, add the Paecilomyces lilacinus microbial inoculum cultivated in step (2), additional proportion is: biological organism material: Paecilomyces lilacinus microbial inoculum=1000: 5, Paecilomyces lilacinus microbial inoculum and biological organism material are fully mixed ferment, leavening temperature is 60-70 DEG C, and fermentation time 5-7 is large;
(5) in the Secondary Fermentation stage: the material in step (4) is carried out stacking Secondary Fermentation, leavening temperature is 30-35 DEG C, keeps biological organism material water rate control 30%, fermentation time: 3-5 days, completes product preparation.
(6) granulation and wrapping process, after testing, the technical indicator of product meets the requirements.
The application of this soil ecology nursing microbial-bacterial fertilizer, apply in Plantula Brassicae chinensis planting greenhouse soil before crop-planting as base manure, every mu of consumption 100kg, eelworm harm obviously reduces, and product increases by 30%.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.Any mark in claim should be considered as the claim involved by limiting.
The above; be only preferred embodiment of the present invention; not in order to limit the present invention, every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalently replace and improve, within the protection domain that all should be included in technical solution of the present invention.
Claims (10)
1. the Synthesis and applications of a soil ecology nursing microbial-bacterial fertilizer, it is characterized in that comprising organic content >=40%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=0.2 hundred million CFU/ gram, wherein Paecilomyces lilacinus number >=0.1 hundred million CFU/ gram.
2. soil ecology nursing microbial-bacterial fertilizer according to claim 1, it is characterized in that containing organic content >=50%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=200,000,000 CFU/ gram, wherein Paecilomyces lilacinus number >=0.2 hundred million CFU/ gram.
3. soil ecology nursing microbial-bacterial fertilizer according to claim 1, it is characterized in that containing organic content >=50%, comprise photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus, Paecilomyces lilacinus, living bacteria count >=500,000,000 CFU/ gram, wherein Paecilomyces lilacinus number >=200,000,000 CFU/ gram.
4. the preparation method of soil ecology nursing microbial-bacterial fertilizer according to claim 1, is characterized in that comprising the following steps:
(1) preparation of Paecilomyces lilacinus liquid spawn: the test tube slant culture medium inoculated Paecilomyces lilacinus pure strain of the suitable Paecilomyces lilacinus growth of preparation, is cultured to and occurs lavender powdery conidium; Again to prepare the one-level test tube slant bacterial classification of the triangular flask liquid nutrient medium inoculation Paecilomyces lilacinus of suitable Paecilomyces lilacinus growth, constant temperature rotating and culturing is stand-by, and water used in above-mentioned culturing process is the high-energy water of molecular oscillation frequency at more than 3000Mhz;
(2) for the production of the enlarged culturing of liquid spawn: molecular oscillation frequency at the high-energy water of more than 3000Mhz as the large fermentation tank substratum of the suitable Paecilomyces lilacinus growth of water source preparation, ripe triangular flask Paecilomyces lilacinus liquid spawn is cultivated by by above-mentioned triangular flask liquid nutrient medium, by in aseptic technique access fermentor tank, continue to pass into sterile air and carry out stir culture;
(3) configuration of substratum: by biological organic matter material and organism decomposing agent according to 100: 0.3-0.5 ratio fully mix, and carry out fermenting, becoming thoroughly decomposed, this organism decomposing agent contains the bacterial classifications such as photosynthetic bacteria, vinelandii, phosphorus bacteria, colloid bacillus cereus;
(4) the Paecilomyces lilacinus microbial inoculum enlarged culturing stage is added: after the biological organism material temperature degree fermenting, become thoroughly decomposed is down to 40 DEG C, add the Paecilomyces lilacinus microbial inoculum cultivated in step (2), additional proportion is: biological organism material: Paecilomyces lilacinus microbial inoculum=1000: 0.5-5, Paecilomyces lilacinus microbial inoculum and biological organism material is fully mixed and ferments;
(5) Secondary Fermentation: the material in step (4) is carried out stacking Secondary Fermentation, leavening temperature is 30-35 DEG C, keeps biological organism material water rate control 30%, fermentation time: 3-5 days, completes product preparation.
5. the preparation method of soil ecology nursing microbial-bacterial fertilizer according to claim 4, it is characterized in that the solid slant culture method of Paecilomyces lilacinus liquid spawn described in step (1) is: configuration Cha Shi solid medium or PDA solid medium, sterilizing, streak inoculation Paecilomyces lilacinus, is placed in constant incubator to be cultured at 25-30 DEG C and occurs lavender powdery conidium; Liquid cultivating method be culture medium prescription with in described solid slope spawn culture based formulas except not adding agar all the other compositions or preparation method identical, on aseptic operating platform, hook from the Paecilomyces lilacinus that solid slant culture base is preserved with transfering loop and get conidium one ring, be seeded in triangular flask in liquid nutrient medium, cultivate 36 hours for shaking table 110 ~ 150rpm, 25-30 DEG C.
6. the preparation method of soil ecology according to claim 4 nursing microbial-bacterial fertilizer, it is characterized in that culture condition described in step (2) be fermentor tank internal pressure is 0.03 MPa, temperature is 25-28 DEG C, and incubation time 54 hours is stand-by.
7. the preparation method of soil ecology nursing microbial-bacterial fertilizer according to claim 4, it is characterized in that biological organic matter material described in step (3) is the mixture of one or more of fowl and animal excrement, agricultural crop straw, bacterium mushroom slag, manioc waste, leavening temperature is 60-70 DEG C, fermentation time 5-7 days.
8. the preparation method of soil ecology nursing microbial-bacterial fertilizer according to claim 4, it is characterized in that continuing fermentation time described in step (4) is 24 hours, leavening temperature is 30-35 DEG C.
9. the preparation method of soil ecology nursing microbial-bacterial fertilizer according to claim 4, is characterized in that aforesaid method can also comprise granulation and wrapping process.
10. the using method of soil ecology according to claim 1 nursing microbial-bacterial fertilizer: using this soil ecology nursing microbial-bacterial fertilizer as during base manure is manured into soil before crop-planting, every mu of consumption is 100-200kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410768533.4A CN104761308A (en) | 2014-12-04 | 2014-12-04 | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410768533.4A CN104761308A (en) | 2014-12-04 | 2014-12-04 | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104761308A true CN104761308A (en) | 2015-07-08 |
Family
ID=53643470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410768533.4A Pending CN104761308A (en) | 2014-12-04 | 2014-12-04 | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104761308A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105061098A (en) * | 2015-07-27 | 2015-11-18 | 侯期任 | Microbial fertilizer for preventing and controlling citrus Huanglongbing, and preparation method thereof |
| CN105199995A (en) * | 2015-10-27 | 2015-12-30 | 山西省农业科学院生物技术研究中心 | Micro-ecological composite inoculant special for overcoming watermelon continuous cropping obstacle and application method |
| CN105541442A (en) * | 2016-02-29 | 2016-05-04 | 河北西马仑生物肥料有限公司 | Continuous cropping soil conditioner and preparation method thereof |
| CN105859347A (en) * | 2016-04-06 | 2016-08-17 | 福建农林大学 | Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof |
| CN106977344A (en) * | 2016-12-26 | 2017-07-25 | 山东美吉多生态农业科技有限公司 | A kind of mulch biological organic fertilizer and preparation method thereof |
| CN107439218A (en) * | 2017-09-22 | 2017-12-08 | 何振贤 | The method for integrated control of warmhouse booth Organic cucumber root knot nematode disease |
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN108243695A (en) * | 2018-01-19 | 2018-07-06 | 贵州省生物研究所 | A kind of Chinese actinidia root maize ear rot pesticide control and its application method |
| CN108484291A (en) * | 2018-06-08 | 2018-09-04 | 沈阳中博环保科技研发有限公司 | Discarded Cordyceps militaris rice cake bio-bacterial manure and production method |
| CN109574765A (en) * | 2018-12-14 | 2019-04-05 | 河北百禾丰化肥有限公司 | A kind of microbial-bacterial fertilizer |
| CN110498714A (en) * | 2019-09-10 | 2019-11-26 | 江苏萤火虫环境科技有限公司 | A kind of preparation method and microbial-bacterial fertilizer of soil ecology nursing microbial-bacterial fertilizer |
| CN110668903A (en) * | 2019-10-23 | 2020-01-10 | 中国农业科学院烟草研究所 | Microbial fertilizer for comprehensive treatment of nematode |
| CN112680366A (en) * | 2021-02-02 | 2021-04-20 | 吉林农业大学 | Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum |
| CN116805175A (en) * | 2023-06-02 | 2023-09-26 | 中哲国际工程设计有限公司 | Medical care building operation and maintenance management system based on CIM technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101139231A (en) * | 2006-09-08 | 2008-03-12 | 中国科学院过程工程研究所 | Method for preparing organic composite fertilizer by micro-wave pretreatment of solid organic waste material |
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN102924133A (en) * | 2012-11-19 | 2013-02-13 | 王天喜 | Method for producing microbial agent by using filter mud of sugar mills through fermentation |
-
2014
- 2014-12-04 CN CN201410768533.4A patent/CN104761308A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101139231A (en) * | 2006-09-08 | 2008-03-12 | 中国科学院过程工程研究所 | Method for preparing organic composite fertilizer by micro-wave pretreatment of solid organic waste material |
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN102924133A (en) * | 2012-11-19 | 2013-02-13 | 王天喜 | Method for producing microbial agent by using filter mud of sugar mills through fermentation |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105061098A (en) * | 2015-07-27 | 2015-11-18 | 侯期任 | Microbial fertilizer for preventing and controlling citrus Huanglongbing, and preparation method thereof |
| CN105199995B (en) * | 2015-10-27 | 2018-08-28 | 山西省农业科学院生物技术研究中心 | One kind overcoming the special micro-ecological complex bacterial agent of watermelon continuous cropping and application process |
| CN105199995A (en) * | 2015-10-27 | 2015-12-30 | 山西省农业科学院生物技术研究中心 | Micro-ecological composite inoculant special for overcoming watermelon continuous cropping obstacle and application method |
| CN105541442A (en) * | 2016-02-29 | 2016-05-04 | 河北西马仑生物肥料有限公司 | Continuous cropping soil conditioner and preparation method thereof |
| CN105859347A (en) * | 2016-04-06 | 2016-08-17 | 福建农林大学 | Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof |
| CN105859347B (en) * | 2016-04-06 | 2019-05-03 | 福建农林大学 | A kind of radix pseudostellariae Special compound microbial fertilizer and the preparation method and application thereof |
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN106977344A (en) * | 2016-12-26 | 2017-07-25 | 山东美吉多生态农业科技有限公司 | A kind of mulch biological organic fertilizer and preparation method thereof |
| CN107439218A (en) * | 2017-09-22 | 2017-12-08 | 何振贤 | The method for integrated control of warmhouse booth Organic cucumber root knot nematode disease |
| CN108243695A (en) * | 2018-01-19 | 2018-07-06 | 贵州省生物研究所 | A kind of Chinese actinidia root maize ear rot pesticide control and its application method |
| CN108484291A (en) * | 2018-06-08 | 2018-09-04 | 沈阳中博环保科技研发有限公司 | Discarded Cordyceps militaris rice cake bio-bacterial manure and production method |
| CN109574765A (en) * | 2018-12-14 | 2019-04-05 | 河北百禾丰化肥有限公司 | A kind of microbial-bacterial fertilizer |
| CN110498714A (en) * | 2019-09-10 | 2019-11-26 | 江苏萤火虫环境科技有限公司 | A kind of preparation method and microbial-bacterial fertilizer of soil ecology nursing microbial-bacterial fertilizer |
| CN110668903A (en) * | 2019-10-23 | 2020-01-10 | 中国农业科学院烟草研究所 | Microbial fertilizer for comprehensive treatment of nematode |
| CN112680366A (en) * | 2021-02-02 | 2021-04-20 | 吉林农业大学 | Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum |
| CN112680366B (en) * | 2021-02-02 | 2023-01-31 | 吉林农业大学 | Liquid culture medium for paecilomyces lilacinus and preparation method of paecilomyces lilacinus microbial inoculum |
| CN116805175A (en) * | 2023-06-02 | 2023-09-26 | 中哲国际工程设计有限公司 | Medical care building operation and maintenance management system based on CIM technology |
| CN116805175B (en) * | 2023-06-02 | 2023-12-26 | 中哲国际工程设计有限公司 | Medical care building operation and maintenance management system based on CIM technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104761308A (en) | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer | |
| CN102718577B (en) | Special organic culturing substrate capable of preventing and treating diseases for peppers and preparation method of special organic culturing substrate | |
| CN102964178B (en) | Composite microbial bactericide, and preparation method and application thereof | |
| CN101696390B (en) | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof | |
| KR101190650B1 (en) | A novel Bacillus velezensis B-42 for multi function | |
| CN101659933B (en) | Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof | |
| US20110214463A1 (en) | Antagonistic bacteria for controlling the fusarium wilt of continuous cropping banana and their microbial organic fertilizer | |
| CN102643148B (en) | Special biological disease-resistant vermin-proof culture medium for flowers and preparation method thereof | |
| AU2019204482A1 (en) | Hydroponic method utilizing beneficial micro-organisms | |
| CN102154156A (en) | Antagonists capable of preventing and controlling tomato bacterial wilt or knot nematode and microbial organic fertilizer thereof | |
| CN108277177B (en) | Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application | |
| CN110283753A (en) | A kind of complex micro organism fungicide and preparation method thereof for clearing up soil poison | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN103320330A (en) | Trichoderma aureoviride strain and application thereof | |
| CN103651582A (en) | Broad-spectrum composite microbial fungicide and preparation method thereof | |
| CN106831015A (en) | A kind of bacillus laterosporus microbial manure and its production and use | |
| CN103420692A (en) | Novel special bio-fertilizer applied in crop transplanting and fix planting period | |
| CN104988094A (en) | Method for manufacturing quinclorac solid degrading inoculant | |
| CN108901755A (en) | A kind of watermelon seedling culturing growth-promoting matrix and preparation method thereof | |
| JP3428851B2 (en) | Organic fermented fertilizer and its use | |
| CN102443559B (en) | Baclillus subtilis used for controlling cotton verticillium wilt and application thereof | |
| CN104719345A (en) | Preparation method of biological preparation for preventing and controlling vegetable nematode disease | |
| CN106431661A (en) | Preparation method and application of Pythium oligandrum microbial fertilizer | |
| CN103275892B (en) | Antagonism actinomycetes for preventing and killing continuous cropping cucumber fusarium wilt and microorganism organic fertilizer with antagonism actinomycetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170524 Address after: Xinghu street Suzhou Industrial Park in Jiangsu province 215123 BioBAY Building No. 218 A Applicant after: Nanjing Civil Engineering (Suzhou) Environmental Rehabilitation Technology Co., Ltd. Address before: 230000, D1 building, Hefei hi tech Limited by Share Ltd, No. 800 Wangjiang West Road, hi tech Zone, Anhui, Hefei 7, China Applicant before: Garden bio tech ltd, Huifeng, Anhui |
|
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150708 |