CN107439218A - The method for integrated control of warmhouse booth Organic cucumber root knot nematode disease - Google Patents
The method for integrated control of warmhouse booth Organic cucumber root knot nematode disease Download PDFInfo
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- CN107439218A CN107439218A CN201710866899.9A CN201710866899A CN107439218A CN 107439218 A CN107439218 A CN 107439218A CN 201710866899 A CN201710866899 A CN 201710866899A CN 107439218 A CN107439218 A CN 107439218A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The present invention provides a kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, and described method includes cultural control；Heavy dressing bio-bacterial manure；Physical control is to utilize root-knot nematode biological characteristics, takes water to fill, high temperature boils in a covered pot over a slow fire the measures such as canopy, Soil tillage, kill root-knot nematode；Biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs.Fertilizer and medicament used in the present invention is environmentally friendly, without any pollution；It is without any side effects to people, storage, to soil without any residual；Fertilizer, medicament and material used used is commercially commercially available from, and plant resources used is very abundant, is easy to get, widely distributed, no matter walks anywhere, can be seen on field, the edge of a field, Around the house, hills hillside etc.；Fertilizer used and the manufacture craft of medicament are simple, and fertilizer is exactly the processing method of peasant convention, and medicament is exactly to be extracted with ethanol, it is not necessary to which machine, equipment, cost are easy to spread also than relatively low.
Description
Technical field
The invention belongs to vegetables production technical field, prevents more particularly to the synthesis of warmhouse booth Organic cucumber root knot nematode disease
Control method.
Background technology
Cucumber root-knot nematode disease is one of Common Diseases in cucumber production, especially in vegetable at protected field cultivation, is often resulted in
Serious economic loss.Root-knot nematode not only causes the direct losses of vegetables, and can aggravate the hair of soil-borne fungus venereal disease evil
It is raw, carry out huge disaster to cucumber production active belt.
Preventing and treating for root-knot nematode, crop rotation are most important farming measures, effect also highly significant.It is but big for greenhouse
Working condition as canopy（Investment is big, input is high, benefit must maximize）Just seem especially difficult that there is larger limitation
Property.Second, the disease-resistant quality of plantation resists the harm of root-knot nematode in which can do limits, but resist due to being limited by various factors
Nematode species seed selection and promoting then seems very slowly, if conventional breeding is due to the deficient restriction of anti-source material, breeding week in addition
The characteristics of phase is long, progress are very slow；And the work of Nematode resistance breeding is carried out still in exploration rank using modern biotechnology
Section（Wish it is bright, 2003）.But just it is few to carry out quantity for this current veriety one, it is impossible to meets production needs, two are limited by kind, are trained
The kind for educating completely high anti-root-knot nematode needs time.Third, cultivating grafting green cucumber seedling, this measure has been implemented in some areas,
Effect is preferable, but because technical reason, a wide range of promote still have not short distance.In physical control, using root-knot nematode biology
Characteristic is learned, takes water filling, high temperature to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage, kills root-knot nematode, but is an ancillary method after all, is planted
The upper people of thing growth period morbidity Zen Me Ban ﹖ currently productions are generally prevented and treated using chemical nematicide agent, but traditional smoked
Steam harm of the using effect " vertical rod takes effect " of agent and non-fumigant chemical agent to nematodiasis and serve very big control action,
And long-term use brings a series of problems, such as residues of pesticides to people and other biological harm, the wildness again of Minor diseases, to ring
The pollution in border etc. increasingly highlights, and such medicament is mostly high poison, persistent pesticide, and many of which is disabled successively.Biology
Prevention Technique can overcome the disadvantages that the deficiency of chemical prevention, but biocontrol bacterial strain is influenceed in soil by the soil variables of complexity, raw
The stability of anti-microbial inoculum cannot be guaranteed, and the factors such as preventive effect is unstable influence, and directly restrict promotion and application.
The content of the invention
In summary, the purpose of the present invention is in view of the above-mentioned problems, a kind of and warmhouse booth Organic cucumber root knot of development
The method for integrated control of nematodiasis, can fundamentally it solve the above problems, specific method is cultural control, and crop rotation is most important
Farming measure, effect also highly significant.But for working condition as warmhouse booth（Investment is big, input is high, benefit is necessary
Maximize）Just seem especially difficult that there is larger limitation.But it can be aggravated in the season neutral gear of main breed to root knot line
The lighter vegetables of parasitosis perception, such as green onion, leek, the kind of garlic class.There are a variety of Fast-growing vegetables, such as Chinese cabbage, spinach class energy quilt
Root-knot nematode endangers, because growth time is short, root-knot nematode it is endangered it is smaller, though can not solve root problem, but can be with
Reduce the quantity of root-knot nematode in plastic shed soil.
The technical scheme is that be accomplished by the following way：
A kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, described prevention and controls comprise the following steps：
Step 1: cultural control, it is possible to reduce the quantity of root-knot nematode in plastic shed soil；
Step 2: heavy dressing bio-bacterial manure, bio-bacterial manure is used when deep ploughing is improved the soil, bio-bacterial manure be common Nong Jia Fei ︰ bio-bacterial manures=
100 ︰ 1 carry out retting, and bio-bacterial manure is per 667m2Apply 2000~2500kg；Deep plough at least 20cm in site preparation, kills most of root
Tie lines worm, root-knot nematode are distributed in 10~15cm of surface soil；
Step 3: physical control is to utilize root-knot nematode biological characteristics, water filling, high temperature is taken to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage,
Kill root-knot nematode；
Step 4: biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, described Organic cucumber root knot
Nematodiasis preventing and treating medicine based on herbs is by the preventing and treating of the Yao Ji ︰ water=pouring roots of 1 ︰ 100~200, by the ︰ of Yao Ji ︰ water=1 during cucumber growth
600~800 sprinkling blade preventing and treatings.
The formula of described Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, by weight, including Exocarpium Juglandis Immaturus
60 parts, 50 parts of ginkgo leaf, Artemisia santonica 30-40 parts, chrysanthemum indicum 30-40 parts, 30 parts of marigold flower, 25 parts of piemarker, tobacco cane 100
Part, 25 parts of corydlis bungeana, 40 parts of pepper, 35 parts of Folium Meliae, 30 parts of wilsonii, 40 parts of yellow cinnamon leaf, 30 parts of rhizoma alismatis, stabilizer and anti-corrosion
Agent；
The preparation method of described medicine based on herbs comprises the following steps：
Step 1: air blast is dried up to embrittlement in 40 DEG C of freeze-day with constant temperature case apparatus by plant material used, with pulverizer by plant
Raw material crushes, standby after 425 μm of sieves excessively；
The crushed material that step 1 is obtained is put into the container that can be sealed, and adds the ethanol solution that volumetric concentration is 30-40%,
The addition of ethanol solution is 3-5 times of crushed material quality, and in the case where temperature is 18-24 DEG C, condition soaks 150-170 hours, enters
Row seepage is extracted, and is carried again toward the 5-10 times of ethanol solution continuation seepage of addition leakage slag charge quality in the leakage slag charge of seepage after extraction
Take, obtain extract solution；
Step 3: the extract solution that step 2 is collected adds 0.8% stabilizer and 1.5% preservative precipitates 12-48 hours, supernatant is taken
Liquid concentrates under 50-60 DEG C of negative pressure, removed the gred, and the extract solution that concentration is made prevents and treats plant source medicinal for Organic cucumber root knot nematode disease
Agent.
Relative to prior art, present invention has the advantages that：
1. the fertilizer and medicament used in method for integrated control of the present invention are environmentally friendly, without any pollution；To people, store without any poison
Side effect, to soil without any residual；
2. the fertilizer, medicament and material used used in the present invention are commercially commercially available from, plant resources used is very rich
Richness, it is easy to get, it is widely distributed, no matter walk anywhere, can be seen on field, the edge of a field, Around the house, hills hillside etc.；
3. the manufacture craft of the fertilizer and medicament used in the present invention is simple, fertilizer is exactly the processing method of peasant convention, and medicament is just
It is to be extracted with ethanol, it is not necessary to which machine, equipment, cost are easy to spread also than relatively low.
4. the economic benefit of the present invention is obvious, compared with the control, prevention effect 83.1-90.2%, yield increase 95.4-
95.6%。
Embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1：
A kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, this method comprise the following steps:
Step 1: cultural control, it is possible to reduce the quantity of root-knot nematode in plastic shed soil；
Step 2: heavy dressing bio-bacterial manure, bio-bacterial manure is used when deep ploughing is improved the soil, and bio-bacterial manure is common Nong Jia Fei ︰ bio-bacterial manures
（Contain Bacillus subtillis, Bacillus megatherium, Dipel composition）=100 ︰ 1 carry out retting, and bio-bacterial manure is per 667m2
Apply 2000kg；Deep plough at least 20cm in site preparation, kills most of root-knot nematode, root-knot nematode be distributed in surface soil 10~
15cm；
Step 3: physical control is to utilize root-knot nematode biological characteristics, water filling, high temperature is taken to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage,
Kill root-knot nematode；
Step 4: biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, described Organic cucumber root knot
Nematodiasis preventing and treating medicine based on herbs is by the preventing and treating of the Yao Ji ︰ water=pouring roots of 1 ︰ 150, or by the ︰ 700 of Yao Ji ︰ water=1 during cucumber growth
Spray blade preventing and treating；
Organic cucumber root knot nematode disease preventing and treating medicine based on herbs specific formula be：By weight, 60 parts of Exocarpium Juglandis Immaturus, ginkgo
50 parts of leaf, 30 parts of Artemisia santonica, 30 parts of chrysanthemum indicum, 30 parts of marigold flower, 25 parts of piemarker, 100 parts of tobacco cane, 25 parts of corydlis bungeana, Hu
40 parts of green pepper, 35 parts of Folium Meliae, 30 parts of wilsonii, 40 parts of yellow cinnamon leaf, 30 parts of rhizoma alismatis, stabilizer and preservative；
The preparation method of described medicine based on herbs comprises the following steps：
Step 1: air blast is dried up to embrittlement in 40 DEG C of freeze-day with constant temperature case apparatus by plant material used, with pulverizer by plant
Raw material crushes, standby after 425 μm of sieves excessively；
Step 2: the crushed material that step 1 is obtained is put into the container that can be sealed, the second that volumetric concentration is 30-40% is added
Alcoholic solution, the addition of ethanol solution are 3-5 times of crushed material quality, and condition soaks 150-170 in the case where temperature is 18-24 DEG C
Hour, seepage extraction is carried out, is continued again toward 5-10 times of ethanol solution of addition leakage slag charge quality in the leakage slag charge of seepage after extraction
Seepage is extracted, and obtains extract solution；
Step 3: the extract solution that step 2 is collected adds 0.8% stabilizer and 1.5% preservative precipitates 12-48 hours, supernatant is taken
Liquid concentrates under 50-60 DEG C of negative pressure, removed the gred, and the extract solution that concentration is made prevents and treats plant source medicinal for Organic cucumber root knot nematode disease
Agent.
Embodiment 2：
A kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, this method comprise the following steps:
Step 1: cultural control, it is possible to reduce the quantity of root-knot nematode in plastic shed soil；
Step 2: heavy dressing bio-bacterial manure, bio-bacterial manure is used when deep ploughing is improved the soil, and bio-bacterial manure is common Nong Jia Fei ︰ bio-bacterial manures
（Contain Bacillus subtillis, Bacillus megatherium, Dipel composition）=100 ︰ 1 carry out retting, and bio-bacterial manure is per 667m2
Apply 2250kg；Deep plough at least 20cm in site preparation, can kill most of root-knot nematode, root-knot nematode be distributed in surface soil 10~
15cm；
Step 3: physical control is to utilize root-knot nematode biological characteristics, water filling, high temperature is taken to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage,
Root-knot nematode can be killed；
Step 4: biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, described Organic cucumber root knot
Nematodiasis preventing and treating medicine based on herbs is An the preventing and treating of the Yao ︰ water=pouring roots of 1 ︰ 100, or is sprayed during cucumber growth An the ︰ 600 of Yao ︰ water=1
Blade is prevented and treated；
Organic cucumber root knot nematode disease preventing and treating medicine based on herbs specific formula be：By weight, 60 parts of Exocarpium Juglandis Immaturus, ginkgo
50 parts of leaf, 35 parts of Artemisia santonica, 35 parts of chrysanthemum indicum, 30 parts of marigold flower, 25 parts of piemarker, 100 parts of tobacco cane, 25 parts of corydlis bungeana, Hu
40 parts of green pepper, 35 parts of Folium Meliae, 30 parts of wilsonii, 40 parts of yellow cinnamon leaf, 30 parts of rhizoma alismatis, stabilizer, preservative；
The preparation method of described medicine based on herbs comprises the following steps：
Step 1: air blast is dried up to embrittlement in 40 DEG C of freeze-day with constant temperature case apparatus by plant material used, with pulverizer by plant
Raw material crushes, standby after 425 μm of sieves excessively；
Step 2: the crushed material that step 1 is obtained is put into the container that can be sealed, the second that volumetric concentration is 30-40% is added
Alcoholic solution, the addition of ethanol solution are 3-5 times of crushed material quality, and condition soaks 150-170 in the case where temperature is 18-24 DEG C
Hour, seepage extraction is carried out, is continued again toward 5-10 times of ethanol solution of addition leakage slag charge quality in the leakage slag charge of seepage after extraction
Seepage is extracted, and obtains extract solution；
Step 3: the extract solution that step 2 is collected adds 0.8% stabilizer and 1.5% preservative precipitates 12-48 hours, supernatant is taken
Liquid concentrates under 50-60 DEG C of negative pressure, removed the gred, and the extract solution that concentration is made prevents and treats plant source medicinal for Organic cucumber root knot nematode disease
Agent.
Embodiment 3：
A kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, this method comprise the following steps:
Step 1: cultural control, it is possible to reduce the quantity of root-knot nematode in plastic shed soil；
Step 2: heavy dressing bio-bacterial manure, bio-bacterial manure is used when deep ploughing is improved the soil, and bio-bacterial manure is common Nong Jia Fei ︰ bio-bacterial manures
（Contain Bacillus subtillis, Bacillus megatherium, Dipel composition）=100 ︰ 1 carry out retting, and bio-bacterial manure is per 667m2
Apply 2500kg；Deep plough at least 20cm in site preparation, can kill most of root-knot nematode, root-knot nematode be distributed in surface soil 10~
15cm；
Step 3: physical control is to utilize root-knot nematode biological characteristics, water filling, high temperature is taken to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage,
Root-knot nematode can be killed；
Step 4: biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, described Organic cucumber root knot
Nematodiasis preventing and treating medicine based on herbs is An the preventing and treating of the Yao ︰ water=pouring roots of 1 ︰ 200, or is sprayed during cucumber growth An the ︰ 800 of Yao ︰ water=1
Blade is prevented and treated；
Organic cucumber root knot nematode disease preventing and treating medicine based on herbs specific formula be：By weight, 60 parts of Exocarpium Juglandis Immaturus, ginkgo
50 parts of leaf, 30 parts of Artemisia santonica, 35 parts of chrysanthemum indicum, 30 parts of marigold flower, 25 parts of piemarker, 100 parts of tobacco cane, 25 parts of corydlis bungeana, Hu
40 parts of green pepper, 35 parts of Folium Meliae, 30 parts of wilsonii, 40 parts of yellow cinnamon leaf, 30 parts of rhizoma alismatis, stabilizer, preservative；
The preparation method of described medicine based on herbs comprises the following steps：
Step 1: air blast is dried up to embrittlement in 40 DEG C of freeze-day with constant temperature case apparatus by plant material used, with pulverizer by plant
Raw material crushes, standby after 425 μm of sieves excessively；
Step 2: the crushed material that step 1 is obtained is put into the container that can be sealed, the second that volumetric concentration is 30-40% is added
Alcoholic solution, the addition of ethanol solution are 3-5 times of crushed material quality, and condition soaks 150-170 in the case where temperature is 18-24 DEG C
Hour, seepage extraction is carried out, is continued again toward 5-10 times of ethanol solution of addition leakage slag charge quality in the leakage slag charge of seepage after extraction
Seepage is extracted, and obtains extract solution；
Step 3: the extract solution that step 2 is collected adds 0.8% stabilizer and 1.5% preservative precipitates 12-48 hours, supernatant is taken
Liquid concentrates under 50-60 DEG C of negative pressure, removed the gred, and the extract solution that concentration is made prevents and treats plant source medicinal for Organic cucumber root knot nematode disease
Agent.
In order to illustrate that the medicament of the present invention to the prevention effect of Cucumber root-knot nematode disease, is tested embodiment 1-3, as a result
It is as follows.
1st, experimental condition：Experimental field it is located in the day-light greenhouse in the agricultural industry garden of Dengfeng City Lu Dian towns reed East Village.
It is repeated 3 times.Often repeat to set four treatment groups：1-3 groups correspond to embodiment 1-3, and the 4th group is control, is 3 × 4m per processing area,
Isolation strip is set between processing；Embodiment 1-3 prepare medicament according to liquid medicine than 1 ︰ 200 dilution liquid irrigating root, every plant of 200ml, the 4th group
Clear water 200ml is filled, is carried out within 30 days in transplanting and after planting respectively, method, dosing are just the same.
2nd, investigation and computational methods：Investigation is carried out for 30 days after pouring root respectively, and often processing takes 10 plants, and sampling point cucumber is connected
Root is dug out, and is rinsed well with clear water, observes root root knot formational situation.Strain grade scale fall ill according to section imperial jade seal（Plant nematode
Learn, in July, 2011, Science Press）Divide 6 grades：0 grade, root system is without root knot；1 grade, root system have a small amount of root knot；2 grades, root knot accounts for always
Root system ratio ﹤ 25%；3 grades, root knot account for total root system ratio 25-50%；4 grades, root knot account for total root system ratio 51-75%；5 grades, root knot
Account for total root system ratio ﹥ 75%.
Root disease index=∑（Old complaint numbers at different levels × relative value of series）/（Investigate total radical × 5）
With respect to prevention effect（%）=（Compare root disease index-processing root disease index）/ processing root disease index
Test data uses Duncan ' s newly multiple through DPS softwares and the software statistics of Micrsofe Office Excel 2003
Extreme difference carries out significance difference analysis.
3rd, result of the test is referring to table 1.
As can be seen from Table 1, prevention effect highly significant of the medicament to Cucumber root-knot nematode disease of the present invention is illustrated.
Claims (3)
- A kind of 1. method for integrated control of warmhouse booth Organic cucumber root knot nematode disease, it is characterised in that described prevention and controls Comprise the following steps：Step 1: cultural control, it is possible to reduce the quantity of root-knot nematode in plastic shed soil；Step 2: heavy dressing bio-bacterial manure, bio-bacterial manure is used when deep ploughing is improved the soil, bio-bacterial manure be common Nong Jia Fei ︰ bio-bacterial manures= 100 ︰ 1 carry out retting, and bio-bacterial manure is per 667m2Apply 2000~2500kg；Deep plough at least 20cm in site preparation, kills most root knots Nematode, root-knot nematode are distributed in 10~15cm of surface soil；Step 3: physical control is to utilize root-knot nematode biological characteristics, water filling, high temperature is taken to boil in a covered pot over a slow fire the measures such as canopy, Soil tillage, Kill root-knot nematode；Step 4: biological control is using Organic cucumber root knot nematode disease preventing and treating medicine based on herbs, described Organic cucumber root knot Nematodiasis preventing and treating medicine based on herbs is by the preventing and treating of the Yao Ji ︰ water=pouring roots of 1 ︰ 100~200, by the ︰ of Yao Ji ︰ water=1 during cucumber growth 600~800 sprinkling blade preventing and treatings.
- 2. a kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease according to claim 1, its feature It is, described Organic cucumber root knot nematode disease prevents and treats the formula of medicine based on herbs, by weight, including Exocarpium Juglandis Immaturus 60 Part, 50 parts of ginkgo leaf, Artemisia santonica 30-40 parts, chrysanthemum indicum 30-40 parts, 30 parts of marigold flower, 25 parts of piemarker, 100 parts of tobacco cane, 25 parts of corydlis bungeana, 40 parts of pepper, 35 parts of Folium Meliae, 30 parts of wilsonii, 40 parts of yellow cinnamon leaf, 30 parts of rhizoma alismatis, stabilizer and preservative.
- 3. a kind of method for integrated control of warmhouse booth Organic cucumber root knot nematode disease according to claim 1 or 2, it is special Sign is that the preparation method of the medicine based on herbs comprises the following steps：Step 1: air blast is dried up to embrittlement in 40 DEG C of freeze-day with constant temperature case apparatus by plant material used, with pulverizer by plant Raw material crushes, standby after 425 μm of sieves excessively；Step 2: the crushed material that step 1 is obtained is put into the container that can be sealed, the second that volumetric concentration is 30-40% is added Alcoholic solution, the addition of ethanol solution are 3-5 times of crushed material quality, and condition soaks 150-170 in the case where temperature is 18-24 DEG C Hour, seepage extraction is carried out, is continued again toward 5-10 times of ethanol solution of addition leakage slag charge quality in the leakage slag charge of seepage after extraction Seepage is extracted, and obtains extract solution；Step 3: the extract solution that step 2 is collected adds 0.8% stabilizer and 1.5% preservative precipitates 12-48 hours, supernatant is taken Liquid concentrates under 50-60 DEG C of negative pressure, removed the gred, and the extract solution that concentration is made prevents and treats plant source medicinal for Organic cucumber root knot nematode disease Agent.
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| CN107960399A (en) * | 2017-12-25 | 2018-04-27 | 南京信息工程大学 | A kind of pesticide concentration control system of greenhouse and use |
| CN109089887A (en) * | 2018-10-26 | 2018-12-28 | 湖南湘研种业有限公司 | A kind of method of anther tissue culture |
| CN109496623A (en) * | 2018-12-07 | 2019-03-22 | 王其俊 | A kind of root knot nematode control method in greenhouse crops plantation |
| CN111567571A (en) * | 2020-05-15 | 2020-08-25 | 湖北大学 | Plant source compound meloidogyne incognita insecticide and preparation method thereof |
| CN112772280A (en) * | 2021-02-07 | 2021-05-11 | 贵州大学 | Dictyophora rubrovalvata-morchella esculenta-vegetable continuous cropping cultivation method |
| CN113812323A (en) * | 2021-09-28 | 2021-12-21 | 云南省农业科学院药用植物研究所 | Method for preventing and controlling pseudo-ginseng nematode disease |
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| CN109496623A (en) * | 2018-12-07 | 2019-03-22 | 王其俊 | A kind of root knot nematode control method in greenhouse crops plantation |
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