JP3428851B2 - Organic fermented fertilizer and its use - Google Patents

Organic fermented fertilizer and its use

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Publication number
JP3428851B2
JP3428851B2 JP06086897A JP6086897A JP3428851B2 JP 3428851 B2 JP3428851 B2 JP 3428851B2 JP 06086897 A JP06086897 A JP 06086897A JP 6086897 A JP6086897 A JP 6086897A JP 3428851 B2 JP3428851 B2 JP 3428851B2
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JP
Japan
Prior art keywords
organic
fermentation
fertilizer
high temperature
rice
Prior art date
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JP06086897A
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JPH10251087A (en
Inventor
照文 荒木
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照文 荒木
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

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  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【発明の属する技術分野】本発明は、農作物の肥料とし
ての効果のみならず、連作障害や紫紋羽病等の予防、雑
草の防除、稲の倒伏防止、線虫の駆除等に有効な有機質
発酵肥料とその使用方法に関し、特に、研究培養の結果
得られた特許微生物寄託センター寄託のAT−3菌を用
いることを特徴とする。
TECHNICAL FIELD The present invention relates to an organic substance effective not only as a fertilizer for agricultural crops, but also for prevention of continuous cropping damage and purple crotch disease, control of weeds, prevention of lodging of rice, control of nematodes, etc. The fermented fertilizer and the method for using the fertilizer are characterized by using AT-3 bacteria deposited by the Patent Microorganism Depositary Center obtained as a result of research culture.

【0002】[0002]

【従来の技術】従来、鶏糞等の発酵には、野積み発酵が
主に行なわれており、発酵温度も発酵熱利用による55〜
60℃が維持されるのが通例であった。この発酵は高温で
の増殖が困難なかび類によるもので、70℃付近の高温に
なると菌が死滅して発酵しなくなるから、70℃付近もの
高温での発酵は行なわれていない。また、発酵後の鶏糞
も肥料としての用途以外には考えられなかったようであ
る。
2. Description of the Related Art Conventionally, for the fermentation of chicken manure and the like, open-field fermentation has been mainly carried out, and the fermentation temperature is 55-
It was customary to maintain 60 ° C. This fermentation is due to molds that are difficult to grow at high temperatures, and at high temperatures around 70 ° C, the bacteria die and do not ferment, so fermentation at temperatures as high as 70 ° C has not been carried out. Also, it seems that fermented chicken manure could not be considered except for its use as a fertilizer.

【0003】[0003]

【発明が解決しようとする課題】本発明者は鶏糞等の発
酵に特殊な菌を用いると、発酵後の鶏糞等が従来にない
作用を発揮するのではないかと考え鋭意検討した。特
に、ブドウ,ナシ,リンゴ,ウメ,イチヂク,モモなどの果
樹を中心に作物の根に寄生し、根を腐敗させて枯死させ
る病気で、最近特にビニールハウス、ガラス温室に発生
しているモンバ(紫紋羽)病は、果樹栽培農家で早急な解
決が待たれていた。この他、稲作の倒伏防止、連作障
害、雑草の発生防止、線虫被害の駆除と果樹園芸等を営
む農家、並びに稲作、畑作農家にとって解決すべき多く
の課題を有している。
DISCLOSURE OF THE INVENTION The present inventor has diligently studied that if fermenting chicken manure or the like uses a special bacterium, chicken manure or the like after fermentation may exert an unprecedented action. In particular, it is a disease that parasitizes the roots of crops, mainly fruit trees such as grapes, pears, apples, plums, figs, and peaches, and rots and kills the roots. For purple herb disease, fruit tree farmers were waiting for immediate solution. In addition to this, there are many problems to be solved for farmers who are involved in prevention of lodging of rice cultivation, obstacles to continuous cropping, prevention of weeds, elimination of nematode damage and fruit gardening, and rice cultivation and field cultivation.

【0004】[0004]

【課題を解決するための手段】従来の鶏糞等の自然委せ
の発酵に代えて、特殊な菌による発酵の結果得られた有
機質発酵肥料が、上記課題を解決するものと考え、種々
検討した結果、鶏糞,牛糞,豚糞,稲藁,落葉の1種又
は2種以上の混合物からなる有機物に対して、工業技術
院生命工学工業技術研究所特許微生物寄託センター(特
許微生物寄託センターと略記)寄託(受託番号 FERM P-16
073、平成9年2月10日受託、受託通知番号 9生寄文第
221号、生存に関する証明書番号も同じ、通知日:平成
9年3月10日)のAT−3菌を混合して、発酵熟成させ
た有機質発酵肥料が上記課題を解決することを確認し
た。また、このAT−3菌は60〜70℃の高温での発酵熟
成を可能とし、これにより得られた有機質発酵肥料も上
記課題を解決することを確認した。
[Means for Solving the Problems] In place of the conventional method of naturally fermenting chicken manure and the like, organic fertilizers obtained as a result of fermentation by a special fungus are considered to solve the above problems, and various studies were conducted. a result, chicken droppings, cow dung, feces, rice straw, one of the deciduous also
For the organic matter consisting of a mixture of two or more kinds , the Institute of Biotechnology, Institute of Industrial Science, Institute of Biotechnology, Patent Microorganism Depositary Center (abbreviated as Patent Microorganism Depositary Center) deposit (accession number FERM P-16
073, Contracted on February 10, 1997 , Contract Notification Number 9
No. 221, the same certificate number for survival, date of notification: Heisei
It was confirmed that an organic fermented fertilizer fermented and aged by mixing AT-3 bacteria (March 10, 1997 ) can solve the above problems. It was also confirmed that this AT-3 bacterium enables fermentation and ripening at a high temperature of 60 to 70 ° C., and the organic fertilizer obtained by this also solves the above problems.

【0005】ここにいう本発明者が培養に成功した特許
微生物寄託センター寄託の高温発酵可能なAT−3菌
は、バチルス・サブチリス(Bacillus subtilis)AT-3とバ
チルス・ステアロサーモフィルス(Bacillus stearotherm
ophilus)AT-3の2種の細菌種の混合物を称す。Bacillus
subtilis AT-3は少なくとも植物病原菌である紋羽病菌
等の真菌に対して抗真菌活性を有する。Bacillus subti
lis AT-3は発育の至適温度が37℃で、Bacillus stearot
hermophilus AT-3は至適温度が55℃である。分類学上は
2種ともバチルス(Bacillus)属の細菌である。このよう
な2種の細菌種の混合により相乗作用をもたらすものと
考えられる。
[0005] The AT-3 bacteria capable of high temperature fermentation deposited by the Patent Microorganism Depositary Center succeeded in culturing by the present inventors are Bacillus subtilis AT-3 and Bacillus stearothermophilus.
ophilus) AT-3 refers to a mixture of two bacterial species. Bacillus
subtilis AT-3 has at least antifungal activity against fungi such as phytopathogenic fungus Monobia. Bacillus subti
The optimal temperature for growth of lis AT-3 is 37 ° C, and Bacillus stearot
The optimum temperature of hermophilus AT-3 is 55 ° C. Taxonomically, both species belong to the genus Bacillus. It is considered that such a mixture of two bacterial species brings about a synergistic effect.

【0006】この相乗作用を利用して、予め85〜95℃で
加熱殺菌後の鶏糞,牛糞,豚糞,稲藁,落葉等の有機物
に対して、至適温度が55℃であるBacillus stearotherm
ophilus AT-3により、まず、60〜70℃の範囲内における
高温での発酵熟成が行われ、これは通常の乳酸菌,嫌気
性菌等が繁殖不可能な温度での発酵であり、これら乳酸
菌や嫌気性菌等の雑菌の繁殖を防ぎながら発酵を可能に
する。次に、至適温度が37℃のBacillus subtilis AT-3
を45〜30℃で低温熟成させる。この時には既に乳酸菌,
嫌気性菌等が前発酵工程での高温により繁殖不可能とな
っており、雑菌の繁殖を防ぎながら発酵を可能にすると
いった理想的な発酵熟成が行われるのである。
Utilizing this synergistic effect, Bacillus stearotherm has an optimum temperature of 55 ° C. for organic substances such as chicken manure, cow manure, pig manure, rice straw, and litter, which have been previously heat-sterilized at 85 to 95 ° C.
With ophilus AT-3, first, fermentation ripening at a high temperature in the range of 60 to 70 ° C is performed. This is fermentation at a temperature at which ordinary lactic acid bacteria and anaerobic bacteria cannot reproduce. Fermentation is possible while preventing the growth of various bacteria such as anaerobic bacteria. Next, Bacillus subtilis AT-3 with an optimum temperature of 37 ° C
Aged at 45-30 ° C at low temperature. By this time the lactic acid bacteria were already
Anaerobic bacteria and the like cannot be propagated due to the high temperature in the pre-fermentation process, and ideal fermentation and aging is performed such that fermentation is possible while preventing the growth of miscellaneous bacteria.

【0007】この有機質発酵肥料を紫紋羽病,連作障
害,雑草防除,稲作の倒伏防止,線虫被害の駆除等の生
じるおそれのある圃場等に用いて予防又は防除を行なう
使用方法についても検討し、好結果を得た。本発明にお
ける有機質発酵肥料には、固形分が主体のものと、水中
に存在させた前記鶏糞等の有機物にAT−3菌を混合し
て、発酵熟成させた液状やペースト状のものがある。
[0007] The use of this organic fermented fertilizer for prevention or control is also investigated in fields where there is a risk of purple purpura rot, continuous cropping damage, weed control, lodging prevention of rice cultivation, control of nematode damage, etc. And got good results. The organic fermented fertilizers of the present invention include those mainly composed of solids, and liquid and pastes obtained by mixing AT-3 bacteria with organic matter such as chicken dung which is present in water and fermenting and aging.

【0008】[0008]

【発明の実施の形態】DETAILED DESCRIPTION OF THE INVENTION

実施例 AT−3菌の培養 鶏糞を常温で堆積して約2週間放置したものの頂上へ白
いチーズ状の菌糸の塊を成長させた。これを寒天培地と
ブイヨンにより孵卵器中で37℃,2日間培養した。ブイ
ヨンの組成は表1の通りである。
Cultured chicken of Example AT-3 was deposited at room temperature and allowed to stand for about 2 weeks, and a white cheese-like mass of hypha was grown on the top of the pile. This was cultured in an incubator at 37 ° C. for 2 days with an agar medium and broth. The composition of broth is shown in Table 1.

【0009】 表1 寒天培地又はブイヨン組成(重量部) 牛脳エキス末 7.5 塩化ナトリウム 5.0 ハートエキス 8.0 リン酸-水素カリウム 2.5 ペプトン 10.0 寒天 15.0 ブドウ糖 2.0 (ブイヨンの時は寒天がない。)[0009]                              Table 1                     Agar medium or broth composition (parts by weight)       Beef brain extract powder 7.5 Sodium chloride 5.0       Heart extract 8.0 Phosphoric acid-potassium hydrogen 2.5       Peptone 10.0 Agar 15.0       Glucose 2.0 (There is no agar in bouillon.)

【0010】形成したコロニーからブイヨン培養を複数
回繰返した後、以下の試験を行なった。生化学的性質の
確認は細菌同定キット(API50CH及びAPI20)による。
After broth culture was repeated a plurality of times from the formed colonies, the following tests were carried out. Confirmation of biochemical properties is by bacterial identification kit (API50CH and API20).

【0011】有機質発酵肥料の製造 公知の縦型(又は横型も可)で通気性かつ撹拌可能な発酵
槽の中へ鶏糞(牛糞等でも構わない)を入れ、まず、85〜
95℃で加熱殺菌した。次に、これを室温に戻したのち、
鶏糞2000Kg当り15KgのAT−3菌を植菌混合し、60℃
で3日間、15分間回転させては30分間止める操作を繰返
して発酵させた。その後3日間37℃で熟成させた。これ
を発酵槽から取出して、以下に示す使用試験を行なっ
た。
Manufacture of Organic Fermentation Fertilizers Put chicken manure (or cow manure etc.) into a well-known vertical (or horizontal) fermentable and breathable fermenter, and then 85-
It sterilized by heating at 95 ° C. Next, after returning it to room temperature,
Inoculate and mix 15kg of AT-3 bacteria per 2000kg of chicken manure, 60 ℃
Fermentation was repeated for 3 days, rotating for 15 minutes and stopping for 30 minutes. After that, it was aged at 37 ° C. for 3 days. This was taken out from the fermenter and subjected to the use test shown below.

【0012】有機質発酵肥料の使用例1 夏栽培ホウレン草の連作障害予防試験に本発明の有機質
発酵肥料を使用した。 試験場所 岡山県真庭郡八束村 試験区:AT−3菌を含む本発明の有機質発酵肥料を10
アール当り300Kg鋤込む。 対照区:クロールピクリンで土壌消毒 ホウレン草は平成7年7月28日に播種し、同年8月24,
27,31日に収穫した。その結果、試験区の収穫 29.3箱 対照区の収穫 23.3箱であり、連作障害防止効果が顕著
であった。
Use Example 1 of Organic Fermented Fertilizer The organic fermented fertilizer of the present invention was used for a test for preventing continuous cropping damage of spinach grown in summer. Test place Yatsuka-mura, Maniwa-gun, Okayama Prefecture: 10 organic fertilizers of the present invention containing AT-3 fungus
Plow 300 kg per are. Control area: Soil disinfecting spinach with chlorpicrin Seed on July 28, 1995, August 24,
Harvested on the 27th and 31st. As a result, the harvest was 29.3 boxes in the test plot and 23.3 boxes in the control plot, and the effect of preventing continuous crop failure was remarkable.

【0013】使用例2 試験場所 岡山県岡山市草ケ部 平成7年3月〜5月の間、モンバ(紫紋羽)病にかかった
ブドウの木の両側の木に、AT−3菌を含む本発明の有
機質発酵肥料を1坪当り1〜2Kgを撒き、水を大量に
散水し、AT−3菌の溶解水が地下50cmに達するまで、
しかも、まんべんなく行き渡るようにした。その結果、
平成9年1月現在、処置を施したブドウの木は1本もモ
ンバ病にかかっていない。
Example of use 2 Test place Sokabe, Okayama City, Okayama Prefecture From March to May 1995, a book containing AT-3 fungus on both sides of a vine affected by Momba (purple herring feather) disease. The organic fertilizer of the invention is sprinkled at 1 to 2 kg per tsubo and sprinkled with a large amount of water until the dissolved water of AT-3 bacteria reaches 50 cm underground.
Moreover, I tried to spread it evenly. as a result,
As of January 1997, none of the treated vines have Momba disease.

【0014】使用例3 ナスの青枯れ病の発生予防試験を行なった。 試験場所 岡山県児島郡灘崎町 試験区:平成6年ナスに青枯れ病の多発した場所にAT
−3菌を含む本発明の有機質発酵肥料を10アール当り45
0Kg鋤込む。 対照区:平成6年には青枯れ病が全く発生していない場
所 この結果、試験区は平成9年1月現在青枯れ病が全く発
生していない。一方、対照区は平成6年には青枯れ病が
全く発生していなかったが、かなりの発生がみられる。
AT−3菌で青枯れ病の病原菌の滅菌が確認された。
Use Example 3 A test for preventing the occurrence of bacterial wilt of eggplant was carried out. Test place Nadasaki-cho, Okayama Prefecture, test area: AT at the place where wilt disease occurred frequently in eggplant in 1994
-The organic fertilizer of the present invention containing 3 fungi is used for 45 per 10 ares.
Plow 0 kg. Control area: a place where no bacterial wilt disease occurred in 1994. As a result, the experimental area has no bacterial wilt disease as of January 1997. On the other hand, in the control plot, wilt disease did not occur at all in 1994, but a considerable occurrence is seen.
Sterilization of bacterial pathogen of bacterial wilt disease was confirmed with AT-3.

【0015】使用例4 除草効果試験を行なった。 試験場所 岡山県岡山市日近 平成8年5月20日に水田に1反当り15Kg入りのAT−3
菌を含む本発明の有機質発酵肥料を20袋を撒布耕耘し
た。20日経て6月10日に田植えした。稲の品種は「九重
糯」、「秋田小町」、「こしひかり」と三品種で試験を行な
った。6月末、7月末、8月末及び10月中旬の収穫時の
それぞれの時期に草が生えるか否かを観察した結果、い
ずれの時期においてもその発生が認められなかった。雑
草の発生を完全に抑制し、また、稲の倒伏防止にも顕著
な効果を発揮した。更に、収穫も対照区に比べて10〜30
%増収となった。
Use Example 4 A herbicidal effect test was conducted. Test place Okayama City, Okayama Prefecture Hikichi AT-3 containing 15 kg per rice paddy on May 20, 1996
20 bags of the organic fermented fertilizer of the present invention containing fungi were spread and cultivated. After 20 days, I planted rice on June 10. The rice varieties were tested in three varieties, including "Kujumaki", "Akita Komachi", and "Koshihikari". As a result of observing whether or not grass grows at the time of harvesting at the end of June, the end of July, the end of August, and the middle of October, the occurrence was not recognized at any time. It completely suppressed the generation of weeds and also showed a remarkable effect in preventing lodging of rice. Furthermore, the harvest is 10-30 compared to the control area.
% Increase in sales.

【0016】線虫駆除試験 平成9年1月20日研究室に於いてAT−3菌の培養液に
線虫を投入し、線虫の動きを観察した。6時間後、線虫
は全く動かなくなった。線虫は死滅していないがAT−
3菌の増殖により活動を停止したものと推察する。この
結果に基づき、線虫の被害の大きい圃場(作物 人参)に
AT−3菌の培養液を3.3m2当り5リットル散布した。
その結果、4箇月経ても線虫の被害の発生が全くなかっ
た。
Nematode Extermination Test On January 20, 1997, nematodes were put into a culture solution of AT-3 bacteria in a laboratory, and the movement of the nematodes was observed. After 6 hours, the nematodes were completely immobile. The nematodes are not dead, but AT-
It is speculated that the activity was stopped due to the growth of 3 bacteria. Based on this result, 5 liters of the AT-3 bacterial culture solution was sprayed per 3.3 m 2 on a field (crops of ginseng) that was heavily damaged by nematodes.
As a result, no nematode damage occurred even after 4 months.

【0017】[0017]

【発明の効果】従来肥料として利用されていた鶏糞に特
殊な高温でも発酵が可能なAT−3菌を混合して発酵す
ることによって、肥料としての効果のみならず、連作障
害や紫紋羽病等の予防、雑草の防除、稲作の倒伏防止,
線虫被害の駆除等に有効な有機質発酵肥料の提供を可能
とした。農薬によらないで農作物や園芸産品の増産を可
能にした意義有る発明である。
EFFECT OF THE INVENTION By mixing and fertilizing chicken manure, which has been conventionally used as a fertilizer, with AT-3 bacteria that can be fermented even at a special high temperature, not only the effect as a fertilizer but also the continuous cropping disorder and purple rot Prevention of weeds, prevention of lodging of rice,
It has made it possible to provide organic fertilizers that are effective in exterminating nematode damage. This is a significant invention that enabled the increase of production of agricultural products and horticultural products without using pesticides.

───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平4−240177(JP,A) 特開 平6−279158(JP,A) 特開 平6−100391(JP,A) 特開 平7−82066(JP,A) 特開 平5−301791(JP,A) 特開 平2−108609(JP,A) 特開 平6−41532(JP,A) 特開 平9−241089(JP,A) 特開 昭57−81894(JP,A) (58)調査した分野(Int.Cl.7,DB名) C05B 1/00 - C05G 5/00 A01N 63/00 - 65/02 ─────────────────────────────────────────────────── ─── Continuation of the front page (56) Reference JP-A-4-240177 (JP, A) JP-A-6-279158 (JP, A) JP-A-6-100391 (JP, A) JP-A-7- 82066 (JP, A) JP 5-301791 (JP, A) JP 2-108609 (JP, A) JP 6-41532 (JP, A) JP 9-241089 (JP, A) JP-A-57-81894 (JP, A) (58) Fields investigated (Int.Cl. 7 , DB name) C05B 1/00-C05G 5/00 A01N 63/00-65/02

Claims (3)

(57)【特許請求の範囲】(57) [Claims] 【請求項1】 鶏糞,牛糞,豚糞,稲藁,落葉の1種又
は2種以上の混合物からなる有機物に対して、工業技術
院生命工学工業技術研究所特許微生物寄託センター寄
(受託番号 FERM P-16073)の高温発酵可能なAT−3菌
であるバチルス・サブチリス(Bacillus subtilis)とバ
チルス・ステアロサーモフィルス(Bacillusstearotherm
ophilus)の2種の細菌種の混合物添加混合して、発酵
熟成させてなる有機質発酵肥料。
1. One of chicken dung, cow dung, pig dung, rice straw, and deciduous leaf , or
Is an industrial technology for organic substances consisting of a mixture of two or more
Hospital life Institute of Advanced Industrial Science and Technology, Patent Microorganisms Depositary Center nearest surrender
AT-3 bacterium capable of high temperature fermentation with ( accession number FERM P-16073)
Bacillus subtilis and Bacillus subtilis
Chilles stearothermophilus
A fermented organic fertilizer obtained by fermenting and aging by adding and mixing a mixture of two bacterial species of (ophilus) .
【請求項2】 高温発酵可能なAT−3菌は予め85〜95
℃で加熱殺菌後の有機物に対して混合し、60〜70℃の範
囲内において高温前発酵させた後、45〜30℃で低温熟成
させてなる請求項1記載の有機質発酵肥料。
2. AT-3 bacteria capable of high temperature fermentation are 85 to 95 in advance.
The fermented organic fertilizer according to claim 1, which is obtained by mixing the organic matter after heat sterilization at ℃, pre-fermenting at a high temperature within a range of 60 to 70 ℃, and aging at a low temperature at 45 to 30 ℃.
【請求項3】 請求項1又は2記載の有機質発酵肥料を
モンバ病,連作障害,雑草防除、稲作の倒伏、線虫被
生じるおそれのある圃場に用いて、これら病害の予防
又は防除を行なうことを特徴とする有機質発酵肥料の使
用方法。
3. A process according to claim 1 or 2 organic fermentation fertilizer Monba disease according, injury by continuous cropping, weed control, lodging of rice, nematodes the damage
Using thefield which may the resulting, use of organic fermentation fertilizer and performing prevention or control of these diseases.
JP06086897A 1997-03-14 1997-03-14 Organic fermented fertilizer and its use Expired - Fee Related JP3428851B2 (en)

Priority Applications (1)

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JP3428851B2 true JP3428851B2 (en) 2003-07-22

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003041257A (en) * 2001-08-01 2003-02-13 Tatsuya Kajikuri Soil-conditioning method
JP5260814B2 (en) * 2001-09-20 2013-08-14 株式会社大川原製作所 Manufacturing method of functional compost
JP2006248886A (en) * 2005-03-11 2006-09-21 Kazue Watanabe Rapid odorless composting method of organic waste such as plant produced material using environmental microorganisms and an oxidizing agent
KR101069219B1 (en) 2009-11-09 2011-09-30 김정이 A fallen leaves pulverizer
GB2478929B (en) 2010-03-23 2013-08-14 Biomax Technologies Pte Ltd Treatment of organic waste
CN102757277B (en) * 2012-07-31 2013-12-04 长春华御实业集团有限公司 Paddy biofertilizer, preparation method thereof and bacillus subtilis
CN102939878B (en) * 2012-10-09 2014-10-08 延边高丽有机大米开发有限公司 Method for cultivating rice
JP6232602B2 (en) * 2013-08-23 2017-11-22 国立大学法人 鹿児島大学 Soil infectious disease control agent and soil infectious disease control method using the same
CN105541423A (en) * 2016-01-06 2016-05-04 长春孙老乐生物科技开发有限公司 Microbial fertilizer containing bacillus subtilis
CN115053762A (en) * 2022-06-20 2022-09-16 阿勒泰戈宝茶股份有限公司 Apocynum venetum plant rejuvenation method and Apocynum venetum rejuvenation microbial preparation

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