CN110343628B - Microbial agent containing candida ethanolate and application thereof - Google Patents
Microbial agent containing candida ethanolate and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial agent containing candida ethanolate, and a preparation method of the microbial agent comprises the following steps: (1) fully dissolving the premixed solid fermentation culture medium by using water; (2) adding 0.01-1 per mill of pre-compounded solid microbial compound bacteria into the fermentation liquor prepared in the step (1), adjusting the pH value to 5-8 by using sodium hydroxide, uniformly stirring, and covering and sealing the container by using a cover; (3) fermenting for 5-9 days at the temperature of 25-40 ℃, and finally adding 10-30 wt% of corn biochemical fulvic acid to obtain the microbial agent. The microbial agent has stable composition and strong universality, has good application effect in the aspect of planting of fruits, vegetables and grain crops, and is convenient for large-scale use.
Description
Technical Field
The invention relates to the technical field of application of microbial agents, in particular to a microbial agent containing candida ethanolica and application thereof.
Background
With the development of green ecological agriculture, the reduction of the use amount of chemical fertilizers and pesticides is in the trend. By applying the organic fertilizer and the microbial preparation, the fertilizer can be partially or even completely replaced, and the dosage of the pesticide is reduced; the edible safety of the agricultural products is powerfully guaranteed while the yield and the quality of the agricultural products are ensured.
The current standard of the organic fertilizer is NY 525-2012, which puts requirements on indexes such as organic matter content, total nutrients and the like in the organic fertilizer. Agricultural microbial preparations are in the development stage, and no clear standard exists.
EM (Effective Microorganisms) is a common compound microbial agent and is compounded by 10 Microorganisms of more than 80, which mainly comprise photosynthetic bacteria, lactic acid bacteria, saccharomycetes and filamentous bacteria. The EM bacteria are easy to live and reproduce, can quickly and stably occupy the dominant position, and form a dominant community of beneficial microbial bacteria. The EM bacteria have the following main functions: organic acid, enzyme and other substances are secreted, and the release of soil nutrient components and the absorption and utilization of plants are promoted; protecting plant cell membrane system, regulating plant metabolism, enhancing immunity of plant, and improving its stress resistance; has the effects of inhibiting and killing soil pathogenic bacteria and preventing and treating diseases; the hormone such as gibberellin is secreted, the speed of activating the plant rooting is accelerated, and the plant rooting, growth, reproduction, flowering and fruiting are promoted. The common use modes of the EM fungicide are as follows: soaking seeds, dressing seeds, soaking roots and spraying leaves; fermenting and composting; spraying soil and topdressing.
The invention patent of the publication No. CN103332971B discloses a lemon ferment liquid fertilizer and a preparation method thereof. The lemon enzyme liquid fertilizer is prepared from the following components in percentage by weight: 40-50% of lemon peel, 30-40% of peanut meal, 10-20% of bone meal, 5-10% of brown sugar, 1-2% of pectin complex enzyme, 1-2% of ferment bacteria, 0.5-1.5% of EM flora and purified water according to the weight ratio of 100: 300. The invention has the beneficial effects that: the soil environment can be improved, the vigorous metabolism of soil microorganisms is promoted, the soil activity is improved, the soil hardening is loosened, the fertility and the disease and bacteria resistance are increased, the cold and drought resistance of crops are enhanced, the soil elements are adsorbed and degraded, the vigorous growth of the crops is promoted, the quality and the yield of the crops are improved, insect pests such as insects and mosquitoes can be reduced, the use of chemical pesticides is avoided as much as possible, and the soil environment-friendly, healthy and low-cost planting method has the advantages of environmental protection, health and low-cost planting.
The invention patent of the grant publication No. CN 104892119B provides a liquid microbial agent for preventing soil hardening and a preparation method thereof. The liquid microbial agent is composed of fulvic acid, brown sugar, urea, aspartic acid, polyacrylamide, EDTA-iron, alginic acid, chinaberry seed extract, rapeseed extract, bacillus subtilis, EM (effective microorganisms), lactic acid bacteria, trichoderma and bacillus mucilaginosus. After the microbial inoculum is used for one year, the volume weight of the soil is 1.0-1.2, the total porosity is 50-55%, the ventilation porosity is 15-22%, and the total amount of heavy metals in the soil is reduced from 600mg/kg of 500-260 mg/kg of 220-260 mg/kg; when the liquid microbial agent is used for planting the linear peppers for 3 years, soil hardening does not occur.
The composite microbial agent and related products which are easy to use and excellent in effect have become market hotspots.
Disclosure of Invention
In view of the above problems in the prior art, the applicant of the present invention provides a microbial agent comprising candida ethanolate and an application thereof. The microbial agent has stable composition and strong universality, has good application effect in the aspect of planting of fruits, vegetables and grain crops, and is convenient for large-scale use.
The technical scheme of the invention is as follows:
a solid microbial composite microbial inoculum containing ethanol candida comprises strains and the content of each strain:
the preservation number of the ethanol Candida (Candida ethanolica) strain is CCTCC NO: M2018633.
A microbial agent containing candida ethanolica is prepared by a method comprising the following steps:
(1) fully dissolving the premixed solid fermentation culture medium by using water;
(2) adding 0.01-1 per mill of pre-compounded solid microbial compound bacteria into the fermentation liquor prepared in the step (1), adjusting the pH value to 5-8 by using sodium hydroxide, uniformly stirring, and covering and sealing the container by using a cover; wherein 0.01-1 per mill is based on the premixed solid fermentation culture medium solution after water dissolution;
(3) fermenting for 5-9 days at the temperature of 25-40 ℃, and finally adding 10-30 wt% of corn biochemical fulvic acid to obtain the microbial agent.
The premixed solid fermentation culture medium solution comprises the following raw materials in percentage by mass: 0.1-5% of glucose, 0.1-5% of brown sugar, 0.1-1% of sodium chloride, 0.01-2% of monopotassium phosphate, 0.01-2% of dipotassium phosphate, 0.01-2% of triammonium citrate and the balance of water.
The pre-compounded solid microbial compound agent contains the following strains and the content of each strain:
the preservation number of the ethanol Candida (Candida ethanolica) strain is CCTCC NO: M2018633.
An application of a microbial agent containing candida ethanolica in planting vegetables, fruits or grain crops.
The application method is irrigation, drip irrigation or foliar spraying.
The beneficial technical effects of the invention are as follows:
the microbial agent has stable composition, strong universality and good application effect, and is convenient for large-scale use.
The microbial agent can be used for planting fruits such as strawberries and vegetables such as Chinese chives, but is not limited to the plants, and is still effective for rice, wheat, barley, Chinese herbal medicines and rhizome plants. Can be used by means of irrigation, drip irrigation, page spraying and the like.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
A preparation method of a candida ethanolica-containing microbial agent comprises the following steps:
(1) fully dissolving the premixed solid fermentation culture medium in a fermentation tank by using water;
(2) adding 0.1 per mill of pre-compounded solid microbial compound inoculant (taking the pre-mixed solid fermentation culture medium solution after water dissolution as a reference) into the culture medium solution prepared in the step (1), adjusting the pH value to 6.5 by using sodium hydroxide, stirring uniformly, and covering and sealing the container by using a cover;
(3) fermenting for 6 days at the temperature of 30 ℃, then adding 20 wt% of corn biochemical fulvic acid, and standing for 1 day to prepare the candida ethanolica-containing microbial agent; its pH value is 3.8, total bacterial count is 2.1X 10 7 cfu/mL, wherein Candida ethanolica is 2X 10 5 cfu/mL。
After the premixed solid fermentation culture medium is fully dissolved by water, the mass percentage of each raw material is shown in table 1:
TABLE 1
| Name of raw materials | Content (wt.) |
| Glucose | 0.5％ |
| Brown sugar | 2％ |
| Sodium chloride | 0.1％ |
| Potassium dihydrogen phosphate | 0.8％ |
| Dipotassium hydrogen phosphate | 1％ |
| Citric acid triammonium salt | 1％ |
| Water (W) | 94.6％ |
The pre-compounded solid microbial compound inoculum consists of strains with the content shown in the table 2:
TABLE 2
The preservation number of the ethanol candida is CCTCC NO: M2018633.
Example 2
A preparation method of a candida ethanolica-containing microbial agent comprises the following steps:
(1) fully dissolving the premixed solid fermentation culture medium in a fermentation tank by using water;
(2) adding 1 per mill of pre-compounded solid microbial compound inoculant (taking the premixed solid fermentation culture medium solution after water dissolution as a reference) into the culture medium solution prepared in the step (1), adjusting the pH value to 5 by using sodium hydroxide, stirring uniformly, and covering and sealing the container by using a cover;
(3) fermenting for 5 days at 40 ℃, then adding 25 wt% of corn biochemical fulvic acid, and standing for 1 day to prepare the candida ethanolica-containing microbial agent; its pH value is 3.5, total bacterial count is 2.6X 10 7 cfu/mL, wherein Candida ethanolica is 6X 10 5 cfu/mL。
After the premixed solid fermentation culture medium is fully dissolved by water, the mass percentage of each raw material is shown in table 3:
TABLE 3
| Name of raw materials | Content (wt.) |
| Glucose | 5％ |
| Brown sugar | 0.1％ |
| Sodium chloride | 0.1％ |
| Potassium dihydrogen phosphate | 1％ |
| Dipotassium hydrogen phosphate | 2％ |
| Citric acid triammonium salt | 0.5％ |
| Water (W) | 91.3％ |
The pre-compounded solid microbial compound inoculum consists of strains with the content shown in the table 4:
TABLE 4
| Bacterial species name | Content (c) of |
| Bacillus licheniformis | 5×10 8 cfu/g |
| Lactobacillus plantarum | 1×10 5 cfu/g |
| Lactobacillus acidophilus | 5×10 8 cfu/g |
| Enterococcus faecalis | 1×10 5 cfu/g |
| Saccharomyces cerevisiae | 1×10 8 cfu/g |
| Candida ethanolica | 1×10 6 cfu/g |
| Candida utilis | 1×10 9 cfu/g |
| Pediococcus pentosaceus | 1×10 5 cfu/g |
| Bifidobacterium | 1×10 9 cfu/g |
| Total number of bacteria | 3.1013×10 9 cfu/g |
The preservation number of the ethanol candida is CCTCC NO: M2018633.
Example 3
A preparation method of a candida ethanolica-containing microbial agent comprises the following steps:
(1) fully dissolving the premixed solid fermentation culture medium in a fermentation vat by using water;
(2) adding 0.5 per mill of pre-compounded solid microbial compound inoculant (taking the pre-mixed solid fermentation culture medium solution after water dissolution as a reference) into the culture medium solution prepared in the step (1), adjusting the pH value of the solution to 8 by using sodium hydroxide, stirring the solution uniformly, and covering and sealing the container by using a cover;
(3) fermenting for 9 days at 25 ℃, then adding 30 wt% of corn biochemical fulvic acid, and standing for 1 day to prepare the candida ethanolica-containing microbial agent; its pH value is 3.5, total bacterial count is 2.4X 10 7 cfu/mL, wherein Candida ethanolica is 4X 10 5 cfu/mL。
After the premixed solid fermentation culture medium is fully dissolved by water, the mass percentage of each raw material is shown in table 5:
TABLE 5
The pre-compounded solid microbial compound inoculum consists of strains with the content shown in the table 6:
TABLE 6
| Bacterial species name | Content (wt.) |
| Bacillus subtilis | 1×10 9 cfu/g |
| Bacillus licheniformis | 1×10 5 cfu/g |
| Lactobacillus plantarum | 1×10 9 cfu/g |
| Lactobacillus acidophilus | 1×10 5 cfu/g |
| Enterococcus faecalis | 1×10 5 cfu/g |
| Saccharomyces cerevisiae | 1×10 8 cfu/g |
| Candida ethanolica | 1×10 6 cfu/g |
| Candida utilis | 1×10 5 cfu/g |
| Pediococcus pentosaceus | 1×10 5 cfu/g |
| Bifidobacterium | 1×10 9 cfu/g |
| Total number of bacteria | 3.105×10 9 cfu/g。 |
The preservation number of the ethanol candida strain is CCTCC NO: M2018633.
Comparative example
Reference is made to example 1, except that Candida ethanolica is not included and the conditions are otherwise the same.
Test example 1
The microbial agent prepared in example 1 was used for strawberry colonization. The planting place is located in Jiangsu Wuxi Anzhen. 4 groups of test groups are set, each group is 200 square meters, and the test groups are respectively marked as 1 group to 4 groups, the row spacing is 20 cm to 25 cm, the plant spacing is 15 cm to 20 cm, and the density is 8000 plants/mu. 150kg of commercial organic fertilizer is applied to the groups 1 to 4. Group 1 was administered with the microbial agent prepared in example 1, group 2 was administered with the microbial agent prepared in comparative example, and group 3 was administered with a commercially available EM liquid (total bacteria count 3X 10) 7 cfu/mL), group 4 was a control, no microbial agent was administered.
The using method of the microbial inoculum comprises the following steps: the dosage is 2 kg/mu/time, each time is 7 days, and the spray is diluted by clear water according to the ratio of 1:100 before use. At the end of one planting season, strawberry harvest statistics are shown in table 7:
TABLE 7
Test example 2
The microbial inoculant prepared in example 1 was used for tomato planting. The planting place is located in Jiangsu Wuxi Anzhen. 4 groups of test groups are set, each group is 200 square meters and is respectively marked as 1 group to 4 groups, the row spacing of a large row is 80cm, the row spacing of a small row is 50cm, the plant spacing is 30cm, and the density is 3000 plants/mu. 250kg of commercial organic fertilizer is applied to groups 1-4. Group 1 was administered with the microbial agent prepared in example 1, group 2 was administered with the microbial agent prepared in comparative example, and group 3 was administered with a commercially available EM liquid (total bacteria count 3X 10) 7 cfu/mL), group 4 was a control, no microbial agent was administered. The using method of the microbial inoculum comprises the following steps: the dosage is 3 kg/mu/time, each time is 7 days, and the spray is diluted by clear water according to the ratio of 1:50 before use. At the end of one planting season, tomato harvest statistics are shown in table 8:
TABLE 8
Test example 3
The microbial agent prepared in example 1 is used for disease control of leeks. The planting place is located in the Xiangshui of Jiangsu. Setting 3 groups of test groups, each group is 30 square meters, respectively recording as groups 1-3, planting leek according to conventional method and managing. Group 1 was administered with the microbial preparation prepared in example 1, and group 2 was administered with a commercial EM liquid (total bacterial count 3X 10) 7 cfu/mL), group 3 was a control, no microbial agent was administered. The using method of the microbial inoculum comprises the following steps: the dosage is 2 kg/mu/time, each time is 10 days, and the spray is diluted by clear water according to the ratio of 1:50 before use. The microbial inoculum control effect is shown in table 9:
TABLE 9
| Incidence of disease | |
| Group 1 | 1.9％ |
| Group 2 | 8.3％ |
| Group 3 | 21.4％ |
Test example 4
The microbial agent prepared in example 1 was used for rice planting at a place located in the northHubei Shibataea. Setting 2 groups of test groups, each group is 2 mu, respectively recording as a group 1-a group 2, and planting and managing the rice according to a conventional method. Group 1 was administered the microbial agent prepared in the examples, and group 2 was used as a control without administration of the microbial agent.
The using method of the microbial inoculum comprises the following steps: (1) firstly diluting the fermentation liquor by 50 times and then taking the diluted fermentation liquor as a base fertilizer; (2) in daily management, the fermentation liquor is diluted by 50 times and used as a foliar fertilizer, the dosage is 3 kilograms per mu per time, and the interval is 10 days.
The rice using the microbial inoculum has dark green and strong leaves and obviously reduced diseases. When harvested, the yield of the group 1 is increased by 8.5 percent compared with the yield of the group 2.
Claims (5)
1. A solid microbial composite microbial inoculum containing ethanol candida is characterized in that the composite microbial inoculum contains strains and the content of each strain is as follows:
the Candida ethanolica (C.) (Candida ethanolica) BacteriaThe seed preservation number is CCTCC NO: M2018633.
2. The microbial agent containing the candida ethanolica is characterized by comprising the following steps:
(1) fully dissolving the premixed solid fermentation culture medium by using water;
(2) adding 0.1 per mill of pre-compounded solid microbial compound bacteria into the fermentation liquor prepared in the step (1), adjusting the pH value to 6.5 by using sodium hydroxide, uniformly stirring, and covering and sealing the container by using a cover; wherein 0.1 per mill is based on the premixed solid fermentation culture medium solution after water dissolution;
(3) fermenting at 30 ℃ for 6 days, finally adding 20 wt% of corn biochemical fulvic acid, and standing for 1 day to prepare the candida ethanolica-containing microbial agent; its pH value is 3.8, total bacterial count is 2.1X 10 7 cfu/mL, wherein Candida ethanolica is 2X 10 5 cfu/mL；
The pre-compounded solid microbial compound agent contains the following strains and the content of each strain:
the Candida ethanolica (C.) (Candida ethanolica) The preservation number of the strain is CCTCC NO: M2018633.
3. The microbial agent according to claim 2, wherein the premix solid fermentation medium solution comprises the following raw materials in percentage by mass: 0.1-5% of glucose, 0.1-5% of brown sugar, 0.1-1% of sodium chloride, 0.01-2% of monopotassium phosphate, 0.01-2% of dipotassium phosphate, 0.01-2% of triammonium citrate and the balance of water.
4. Use of the candida ethanolica-containing microbial inoculant according to claim 2, for the cultivation of vegetables, fruits or food crops.
5. The use according to claim 4, wherein the application method is irrigation, drip irrigation, or foliar spraying.
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| CN110713952B (en) * | 2019-11-12 | 2021-09-03 | 南宁市甜蜜蜜饲料有限公司 | Probiotic-herbaceous plant composite solid fermentation agent and application thereof |
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