CN106947723A - A kind of microbial bacterial agent and its application - Google Patents
A kind of microbial bacterial agent and its application Download PDFInfo
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- CN106947723A CN106947723A CN201710356716.9A CN201710356716A CN106947723A CN 106947723 A CN106947723 A CN 106947723A CN 201710356716 A CN201710356716 A CN 201710356716A CN 106947723 A CN106947723 A CN 106947723A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- General Chemical & Material Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
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- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to field of microbial fermentation, and in particular to a kind of microbial bacterial agent and its application.The microbial bacterial agent includes class cavings lactobacillus, Clavisporalusitaniae, photosynthetic bacteria, bacillus subtilis and actinomyces.The microbial bacterial agent that the present invention is provided can act synergistically, and promote plant growth, regulate and control crop metabolism, promote soil granular to be formed, and improved soil structure increases soil fertility, reaches increasing both production and income, improves the effect of crop quality and resistant to diseases and insects.
Description
Technical field
The present invention relates to field of microbial fermentation, and in particular to a kind of microbial bacterial agent and its application.
Background technology
Concern and food-safe concern pay attention to day by day that people remain to plant pesticide.Soil improvement is production Gao Pin
A kind of effective approach of matter crops, is the bad quality and structure for soil, takes corresponding physics, biological or chemical
Measure, improves soil property, increases soil fertility, and increases crop yield, and improve the process of human survival soil environment.
Probiotics in biological amelioration turns into an important member in modern agricultural development, due to its environmental protection
Characteristic favored by more and more people.Microbial bacterial agent is used for preparing organic fertilizer for agricultural production also increasingly by people
Attention.
Modern increasing people is realizing the plantation of green pollution-free, and increasing people's selection at present is used
Probiotics realizes green planting, and still, microbial inoculum species in the market is various, in some composite bacteria agents in efficient bacterium
Species is less, only two or three, and such composite bacteria agent species is few, the effect of stable fermentation is extremely difficult to, so that can not
Reach good improved soil and make the purpose of high-quality organic fertilizer；Also there is the species of efficient bacterium in composite bacteria agent various,
More than ten kinds, because the interaction principle between each strain is indefinite, the effect reached is difficult to predict.
The content of the invention
It is an object of the invention to according to above shortcomings in the prior art, there is provided a kind of microbial bacterial agent.
Another object of the present invention is the application for providing mentioned microorganism microbial inoculum in soil conditioner.
Another object of the present invention is to provide application of the mentioned microorganism microbial inoculum in organic fertilizer.
Above-mentioned purpose of the present invention is achieved by the following technical programs：
According to the microbial bacterial agent of the embodiment of the present invention, the microbial bacterial agent includes class cavings lactobacillus
(Lactobacillus parafarraginis ZH1), Clavisporalusitaniae (Clavispora lusitaniae) is photosynthetic
Bacterium (Photosynthetic Bacteria), bacillus subtilis (Bacillus subtilis) and actinomyces
(Actinomycete)。
According to the microbial bacterial agent of the embodiment of the present invention, it is preferable that also including culture medium, the culture medium is
Dusty yeast, peptone, corn flour, wheat bran and physiological saline.
According to the present invention embodiment microbial bacterial agent, it is preferable that the content of the microbial bacterial agent for >=
3.5 hundred million/mL.
According to the soil conditioner of the embodiment of the present invention, including microbial bacterial agent as described above.
According to the organic fertilizer of the embodiment of the present invention, including microbial bacterial agent as described above.
According to the organic fertilizer of the embodiment of the present invention, it is preferable that also fine including animal wastes and/or plant
Dimension.
According to the organic fertilizer of the embodiment of the present invention, it is preferable that the animal wastes are poultry animal wastes,
The poultry animal wastes include the one or more in cow dung, pig manure, sheep manure and/or chicken manure；The string includes wheat
One or more in stalk, wood chip, rice husk, cavings and/or bamboo scraps.
According to the preparation method of the organic fertilizer of the embodiment of the present invention, it the described method comprises the following steps：
(1) animal wastes and/or string and the microbial bacterial agent are well mixed；
(2) fermented mixture for obtaining step (1), under 50-60 DEG C of fermentation temperature, heat-preservation fermentation 20-30 days is obtained
The organic fertilizer.
Present invention also offers application of the microbial bacterial agent in soil improvement.According to the embodiment of the present invention,
The microbial bacterial agent is used to make seedling medium, and specific application method is that spray concentration is 1-2% into culture medium raw material
The microbial bacterial agent, and water keep nursery vessel in soil water content in 40-55%, with plastic film seal, composting
Fermentation 20-40 days, that is, obtain the seedling medium.
Present invention also offers application of the microbial bacterial agent in Crops in Applying Fertilizer, during crop growth, with reference to pouring
Water carries out pouring root with the microbial bacterial agent.Also can be during crop growth, the microbial bacterial agent is diluted into concentration is
Hundred million/L of 3.5-6 carry out foliage spray fertilising to crops.
The microbial bacterial agent that the present invention is provided can act synergistically, and play fixed nitrogen, phosphorus decomposing, potassium decomposing, secretion auxin, cell
The effect of the plant hormones such as mitogen, gibberellin, heteroauxin, so as to promote plant growth, regulates and controls crop metabolism, and can be
Rhizosphere amount reproduction, produces mucopolysaccharide, promotes soil granular to be formed, and improved soil structure increases soil fertility, promotes crops
Growth and well developed root system improve with absorbing and set strong, reach increasing both production and income, improve the effect of crop quality and resistant to diseases and insects；This
The microbial bacterial agent that invention is provided also secretes various active enzyme and protein, cracking worm's ovum and harmful levels of pathogens cell membrane, and kill has
Opportunistic pathogen of falling ill and worm's ovum, are development green, organic agriculture, production green, the preferable production green organic food of organic food.
The present invention effective effect be：
1. pass through class cavings lactobacillus (Lactobacillus parafarraginis ZH1), Clavisporalusitaniae
(Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria), bacillus subtilis (Bacillus
Subtilis) the mutual cooperation between actinomyces (Actinomycete), reaches that promotion decomposition of soil organic matter is improved jointly
Soil fertility improves soil texture increase soil beneficial microbe colony, suppresses pathogenic microorganism, and prevention reduces the hair of pest and disease damage
It is raw, reduce chemical fertilizer, Pesticide use amount.
2. the microbial bacterial agent that the present invention is provided can secrete various plants hormone, their collective effects can strengthen plant
Metabolic function, promotes germination, and rhizome is flourishing, while photosynthetic bacteria can improve a variety of micro- in photosynthesis, microbial bacterial agent
Biological collective effect promotes plant prematurity, and many results, the maturity period shifts to an earlier date more than 17-28 days, gourd, fruit and vegetable is increased production 20-
30%, improve the quality and mouthfeel of gourd, fruit and vegetable；Microbial bacterial agent can produce lactic acid, heteroauxin isoequilibrium soil pH value,
The rotten stem of black root is prevented and treated, plant red root disease, acute wilting mitigates crop physiological disease.
3. the microbial bacterial agent that the present invention is provided can produce mucopolysaccharide, soil granular is promoted to be formed, improved soil structure,
Increase soil fertility, fixed nitrogen, phosphorus decomposing, potassium decomposing improve organic matter to the phosphorus in soil, the water conservation and permeability for improving soil disappear
Except soil hardening.
4. the microbial bacterial agent that the present invention is provided can strengthen antibiotic property, increase soil beneficial microbe colony, effectively suppression
Pathogen processed, pre- preventing disease and pest occurs, and reduces pesticide dosage, effectively improves resistivity of the plant to poor environments such as arids.
5. the organic fertilizer of the microbial bacterial agent making provided using the present invention the chemical fertilizer common with market is more environmentally-friendly, this hair
The organic fertilizer of bright offer can make the composition of soil effective K improve 17%, soil fertility is become better and better, not only improve beneficial
The multiplication of microorganism, infiltration, water conservation is also beneficial to the suitable gas enhancing of soil, promotes soil to evolve, can gradually decrease or generation
The pollution-free pure green of the fat existing planting industry of alternative, the crop for coming out plantation more meets the requirement of pollution-free food, adds agriculture
The economic benefit of crop commercially.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belongs to the scope that the present invention is protected.
Embodiment 1
A kind of microbial bacterial agent, including class cavings lactobacillus (Lactobacillus parafarraginis ZH1), Portugal
Grape tooth rod spore yeast (Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria), withered grass gemma
Bacillus (Bacillus subtilis) and actinomyces (Actinomycete).
The content of effective bacterium is 3.5 hundred million/mL in mentioned microorganism microbial inoculum.
Application using above-mentioned microbial bacterial agent as soil conditioner in soil improvement, adds into above-mentioned microbial bacterial agent
Enter the water of 1000 times of parts by weight, obtain the microbial bacterial agent of uniform sprinkling 5000L in microbial bacterial agent dilution, every mu of ground
Dilution.
Using above-mentioned microbial bacterial agent as foliar fertilizer, mentioned microorganism microbial inoculum is diluted 1000 times, dilution is obtained, planted
Thing grows second true leaf and started, and above-mentioned dilution is sprayed on plant leaf surface as foliar fertilizer.
Embodiment 2
A kind of microbial bacterial agent, including class cavings lactobacillus (Lactobacillus parafarraginis ZH1), Portugal
Grape tooth rod spore yeast (Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria), withered grass gemma
Bacillus (Bacillus subtilis) and actinomyces (Actinomycete), dusty yeast, peptone, corn flour, wheat bran and physiology
Salt solution.
The content of effective bacterium is 400,000,000/mL in mentioned microorganism microbial inoculum.
Organic fertilizer is made using mentioned microorganism bacteria fermentation cow dung and chicken manure, is comprised the following steps：
(1) cow dung, chicken manure and the microbial bacterial agent are well mixed；
(2) fermented mixture for obtaining step (1), when fermentation temperature is 50 DEG C, heat-preservation fermentation 30 days obtains described
Organic fertilizer.
Embodiment 3
A kind of microbial bacterial agent, including class cavings lactobacillus (Lactobacillus parafarraginis ZH1), Portugal
Grape tooth rod spore yeast (Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria), withered grass gemma
Bacillus (Bacillus subtilis) and actinomyces (Actinomycete), dusty yeast, peptone, corn flour, wheat bran and physiology
Salt solution.
The content of effective bacterium is 500,000,000/mL in mentioned microorganism microbial inoculum.
Organic fertilizer is made using mentioned microorganism bacteria fermentation pig manure, chicken manure, Wheat Straw, rice husk and cavings, including it is following
Step：
(1) by micro- life described in 0.5kg pig manures, 0.5kg chicken manures, 0.5kg Wheat Straws, 0.5kg rice husks, 0.5kg cavings and 1L
Thing microbial inoculum is well mixed；
(2) fermented mixture for obtaining step (1), when fermentation temperature is 60 DEG C, heat-preservation fermentation 20 days then will hair
The end-product dehumidifying of ferment, granulator granulation, are made organic fertiliser granulates, wherein temperature is no more than during dehumidifying and granulation
65℃。
Embodiment 4
A kind of microbial bacterial agent, including class cavings lactobacillus (Lactobacillus parafarraginis ZH1), Portugal
Grape tooth rod spore yeast (Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria), withered grass gemma
Bacillus (Bacillus subtilis) and actinomyces (Actinomycete).
The content of effective bacterium is 400,000,000/mL in mentioned microorganism microbial inoculum.
Mentioned microorganism microbial inoculum is applied to make seedling medium, comprises the following steps, and adds and uses into seedling medium raw material
Water dilutes 1000 times of mentioned microorganism microbial inoculum, and the water content for keeping culture medium is 40%, with plastic film seal, banks up fermentation 30
My god, obtain seedling medium.
According to the microbial bacterial agent of GB20287-2006 standard detection embodiment 1,1 the results are shown in Table.
The testing result of the microbial bacterial agent of 1 embodiment of table 1
From the result of table 1 it will be seen that the living bacteria count, mould for the microbial bacterial agent that the embodiment of the present invention 1 is provided
Bacterium miscellaneous bacteria number, miscellaneous bacteria rate and pH value all meet the regulation of GB, and miscellaneous bacteria rate is far below GB.
It is used for soil improvement using the microbial bacterial agent of above-described embodiment 1, wherein the experimental plot that the present invention is selected is
Primary standard soil environment as defined in GB15618-1995,2 are the results are shown in Table using the Soil K+adsorption after 2 years.
The mentioned microorganism microbial inoculum of table 2 is used for the use result of soil improvement
From Table 2, it can be seen that being used to reduce soil as soil conditioner using the microbial bacterial agent that the present invention is provided
The content of middle heavy metal element, serves significantly effect, and the content of heavy metals in soil is substantially reduced, and residues of pesticides are not
Detect, the microbial bacterial agent that the present invention is provided has reached decomposition heavy metals in soil by mutually cooperateing with, jointly, improve
The content of N P and K in soil.The microbial bacterial agent that the present invention is provided simultaneously also secretes various active enzyme and protein, cracks
Worm's ovum and harmful levels of pathogens cell membrane, kill harmful pathogen and worm's ovum, reduce the use for even replacing agricultural chemicals.
The organic fertilizer that the embodiment of the present invention 3 is provided is detected according to GB20287-2006, what is thus produced is multiple batches of organic
The coli-group number of fertilizer<100/g, induced worm egg death rate>95%, the content of organic matter (butt)>55%, total nitrogen >=4.5%,
Total phosphorus >=6.5%, total potassium >=5.5%, is up to state standards.Thus the multiple batches of organic fertilizer produced in the application can be steady
Determine, be efficiently used.Microorganism species can effectively decompose animal wastes and plant in the organic fertilizer that this explanation present invention is provided
Fiber, improves the content of N P and K in organic fertilizer, while worm's ovum and harmful microorganism can be killed.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of microbial bacterial agent, it is characterised in that including class cavings lactobacillus (Lactobacillus parafarraginis
ZH1), Clavisporalusitaniae (Clavispora lusitaniae), photosynthetic bacteria (Photosynthetic Bacteria) is withered
Careless bacillus (Bacillus subtilis) and actinomyces (Actinomycete).
2. microbial bacterial agent according to claim 1, it is characterised in that also including culture medium, the culture medium is yeast
Powder, peptone, corn flour, wheat bran and physiological saline.
3. microbial bacterial agent according to claim 1, it is characterised in that the content of effective bacterium in the microbial bacterial agent >=
3.5 hundred million/mL.
4. soil conditioner, it is characterised in that including the microbial bacterial agent described in claim 1.
5. organic fertilizer, it is characterised in that including the microbial bacterial agent described in claim 1.
6. organic fertilizer according to claim 5, it is characterised in that also including animal wastes and/or string.
7. organic fertilizer according to claim 6, it is characterised in that the animal wastes are poultry animal wastes, the fowl
Raiseeing animal wastes includes the one or more in cow dung, pig manure, sheep manure and/or chicken manure；The string includes Wheat Straw, wood
One or more in bits, rice husk, cavings and/or bamboo scraps.
8. the preparation method of the organic fertilizer described in claim 5, it is characterised in that the described method comprises the following steps：
(1) animal wastes and/or string and the microbial bacterial agent are well mixed；
(2) fermented mixture for obtaining step (1), under 50-60 DEG C of fermentation temperature, heat-preservation fermentation 20-30 days obtains described
Organic fertilizer.
9. application of the microbial bacterial agent described in claim 1 in soil improvement.
10. application of the microbial bacterial agent described in claim 1 in Crops in Applying Fertilizer.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107455243A (en) * | 2017-08-13 | 2017-12-12 | 浙江省农业科学院 | A kind of seedling medium and its preparation method and application |
| CN107488078A (en) * | 2017-08-10 | 2017-12-19 | 黑龙江九穗谷农业科技发展有限公司 | A kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria |
| CN107594070A (en) * | 2017-09-08 | 2018-01-19 | 四川亿客农食品有限公司 | A kind of breeding method of Se-rich farm products and its application |
| CN107628881A (en) * | 2017-10-31 | 2018-01-26 | 博白县富山水果种植专业合作社 | A kind of insect prevention base manure of lichee and preparation method thereof |
| CN107857611A (en) * | 2017-11-24 | 2018-03-30 | 马新世 | A kind of corps nutrient pillow and preparation method thereof |
| CN108718578A (en) * | 2018-04-17 | 2018-11-02 | 仁维国际股份有限公司 | Soil improvement method |
| CN109136150A (en) * | 2018-09-21 | 2019-01-04 | 郭春灵 | A kind of complex microorganism liquid bacterial agent and its application |
| CN109735342A (en) * | 2017-12-08 | 2019-05-10 | 苏州市农业科学院 | The method that the acidified soil for being implanted with acidproof crop to kind launches acidified soil conditioner |
| CN110438019A (en) * | 2019-06-06 | 2019-11-12 | 浙江工业大学 | A kind of composite bacteria agent and its fermented garbage from kitchen prepare the application of organic liquid fertilizer |
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| CN103214320A (en) * | 2013-04-18 | 2013-07-24 | 淮安柴米河农业科技发展有限公司 | Soil improvement matrix for alleviating soil continuous cropping obstacles and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488078A (en) * | 2017-08-10 | 2017-12-19 | 黑龙江九穗谷农业科技发展有限公司 | A kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria |
| CN107455243A (en) * | 2017-08-13 | 2017-12-12 | 浙江省农业科学院 | A kind of seedling medium and its preparation method and application |
| CN107594070A (en) * | 2017-09-08 | 2018-01-19 | 四川亿客农食品有限公司 | A kind of breeding method of Se-rich farm products and its application |
| CN107628881A (en) * | 2017-10-31 | 2018-01-26 | 博白县富山水果种植专业合作社 | A kind of insect prevention base manure of lichee and preparation method thereof |
| CN107857611A (en) * | 2017-11-24 | 2018-03-30 | 马新世 | A kind of corps nutrient pillow and preparation method thereof |
| CN109735342A (en) * | 2017-12-08 | 2019-05-10 | 苏州市农业科学院 | The method that the acidified soil for being implanted with acidproof crop to kind launches acidified soil conditioner |
| CN108718578A (en) * | 2018-04-17 | 2018-11-02 | 仁维国际股份有限公司 | Soil improvement method |
| CN109136150A (en) * | 2018-09-21 | 2019-01-04 | 郭春灵 | A kind of complex microorganism liquid bacterial agent and its application |
| CN110438019A (en) * | 2019-06-06 | 2019-11-12 | 浙江工业大学 | A kind of composite bacteria agent and its fermented garbage from kitchen prepare the application of organic liquid fertilizer |
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Application publication date: 20170714 |