CN109136150A - A kind of complex microorganism liquid bacterial agent and its application - Google Patents
A kind of complex microorganism liquid bacterial agent and its application Download PDFInfo
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- CN109136150A CN109136150A CN201811117632.0A CN201811117632A CN109136150A CN 109136150 A CN109136150 A CN 109136150A CN 201811117632 A CN201811117632 A CN 201811117632A CN 109136150 A CN109136150 A CN 109136150A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The present invention provides a kind of complex microorganism liquid bacterial agent and its applications, belong to microorganisms technical field.The complex microorganism liquid bacterial agent includes the component of following mass parts: 24~40 portions of brown sugar, 2~6 parts of glucose, 1~3 part of peptone, 1~3 part of yeast extract, 75~125 parts of compound bacterias and 1500~2200 parts of water；The compound bacteria includes Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria, effective viable bacteria total amount >=1 × 10 in the compound bacteria8CFU/g.Complex microorganism liquid bacterial agent provided by the invention is not influenced by soil environment, and compound bacteria can quickly be bred using the nutritional ingredient of microbial inoculum itself, capture the ecological niche in soil, substantially reduces harmful bacteria breeding.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of complex microorganism liquid bacterial agent and its application.
Background technique
In China, many remote districts can not be realized and apply fertilizers scientifically, it is such as long-term a large amount of using not decomposed chicken manure and
Long-term single use chemical fertilizer, be easy to cause soil environment to destroy, and the soil issues such as hardened, salination, acid-base disorder occurs.
Since there are problems for soil, it is easy to cause crop to take root and not support root, root system buries i.e. extremely after bearing, and what cannot be survived very well asks
Topic.Studies have shown that yellow leaf, leaflet, rotten, dead, root rot, root knot nematode disease, the crops illness such as fruit paint face all with soil
Earth problem is related.
For many years, microbial bacterial agent is constantly subjected to the heat handful of government department supported energetically with dealers, can actually make
It is but and pessimistic with situation.Currently, the microbial bacterial agent promoted in the market is mostly single or composite bacteria agent, acts on single, effect
It is unstable, be manured into soil in enriched degree by the pH value and organic matter of soil shadow it is also very big.If edaphic condition is suitable,
Microbial bacterial agent effect is obvious；Conversely, microorganism own survival is difficult, also just it is difficult to play expected effect.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of effect stability, suitable for the composite microbial thing liquid of the betterment of land
Body microbial inoculum and its application.
In order to achieve the above-mentioned object of the invention, the present invention provides a kind of complex microorganism liquid bacterial agents, including following quality
The component of part: 24~40 portions of brown sugar, 2~6 parts of glucose, 1~3 part of peptone, 1~3 part of yeast extract, 75~125 parts of compound bacterias
With 1500~2200 parts of water；The compound bacteria includes Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria, institute
State effective viable bacteria total amount >=1 × 10 in compound bacteria8CFU/g。
Preferably, in the compound bacteria, the Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria
Effective viable bacteria ratio be 6~10:2~6:1~3:2~6.
Preferably, in the compound bacteria, the living bacteria count of the Lactobacillus rhamnosus is 1~3 × 108CFU/g；Institute
The living bacteria count for stating bacillus subtilis is 0.5~1.5 × 108CFU/g；The living bacteria count of the saccharomycete be 0.25~
0.75×108CFU/g；The living bacteria count of the photosynthetic bacteria is 0.5~1.5 × 108CFU/g。
Preferably, the complex microorganism liquid bacterial agent includes the component of following mass parts: 32 portions of brown sugar, 4 parts of glucose,
2 parts of peptones, 2 parts of yeast extracts, 100 parts of compound bacterias and 1860 parts of water.
The present invention also provides above-mentioned complex microorganism liquid bacterial agents in improvement soil, resists corps diseases, promotion agriculture
Application in crop maturity or raising crop yield.
Preferably, the corps diseases include yellow leaf, fruit paint face, root knot nematode disease, rotten, dead, root-rot and root
Any one or more in cancer.
Preferably, the application is that complex microorganism liquid bacterial agent is applied to soil with 5~20kg/ mus every time of amount.
Preferably, the application temperature of the complex microorganism liquid bacterial agent is 8~35 DEG C.
Preferably, the Dressing date of the complex microorganism liquid bacterial agent is after crop plant is colonized to fruit maturation
Before.
Preferably, the frequency of administration of the complex microorganism liquid bacterial agent is 20~30 days primary.
The utility model has the advantages that the present invention provides a kind of complex microorganism liquid bacterial agent, the component including following mass parts: 24~
40 portions of brown sugar, 2~6 parts of glucose, 1~3 part of peptone, 1~3 part of yeast extract, 75~125 parts of compound bacterias and 1500~2200 parts
Water；The compound bacteria includes Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria, having in the compound bacteria
Imitate viable bacteria total amount >=1 × 108CFU/g.In the present invention, the compound bacteria can secrete hormone, enzyme necessary to various crop, tool
Irritating plant growth, the function of improving utilization rate of fertilizer, and effect is stablized compared with single culture.The present invention by the compound bacteria with
Brown sugar, glucose, peptone and yeast extract, which compound, is made complex microorganism liquid bacterial agent, by the complex microorganism liquid bacterial agent
It is applied to soil to be improved, improvement soil can be played, abolish function that is hardened, reducing moss.Wherein, the brown sugar, glucose,
Peptone and yeast extract provide carbon source, nitrogen source and growth elements abundant for compound bacteria, microorganism can be made preferably to breed；
Meanwhile these components provide large amount of organic again for crop, are conducive to the content for increasing crop chlorophyll.In addition, in soil
A large amount of beneficial bacteriums capture ecological niche, can substantially reduce the breeding of harmful bacteria.
Detailed description of the invention
Fig. 1-A for experimental group asparagus described in the embodiment of the present invention 8 photo；
Fig. 1-B for control group asparagus belonging to the embodiment of the present invention 8 photo.
Specific embodiment
The present invention provides a kind of complex microorganism liquid bacterial agent, including brown sugar, glucose, peptone, yeast extract, water and
Compound bacteria.
In the present invention, the compound bacteria is activated by each original strain, it is numerous, quantitative to expand, is mixed to prepare.
In the present invention, the compound bacteria includes Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria,
The Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria effective viable bacteria ratio be 6~10:2~6:1~
3:2~6, effective viable bacteria total amount >=1 × 10 in the compound bacteria8CFU/g, preferably >=3 × 108CFU/g。
In the present invention, the compound bacteria includes Lactobacillus rhamnosus, and in the present invention, the Lactobacillus rhamnosus has
Imitating viable count is preferably 1~3 × 108CFU/g, more preferably 2 × 108CFU/g.Heretofore described Lactobacillus rhamnosus is used for
Crop can be attached to rapidly crop root, capture ecological niche；Crop dimension beam can be dredged again, to reduce crop disease.
In the present invention, the compound bacteria includes bacillus subtilis, and in the present invention, the bacillus subtilis has
Imitating viable count is preferably 0.5~1.5 × 108CFU/g, more preferably 1 × 108CFU/g.Heretofore described bacillus subtilis
The harmful bacteria in soil can be killed, layer protecting film is formed in crop surface, the intrusion of germ can be inhibited well.
In the present invention, the compound bacteria includes saccharomycete, and in the present invention, the living bacteria count of the saccharomycete is preferred
It is 0.25~0.75 × 108CFU/g, more preferably 0.5 × 108CFU/g.Heretofore described saccharomycete can make loosing soil saturating
Gas is conducive to microorganism and crop breathing, to be conducive to take root.
In the present invention, the compound bacteria includes photosynthetic bacteria, in the present invention, the living bacteria count of the photosynthetic bacteria
Preferably 0.5~1.5 × 108CFU/g, more preferably 1 × 108CFU/g.Heretofore described photosynthetic bacteria can help crop into
Row photosynthesis, improves the yield and quality.
The present invention proportions above-mentioned four bacterium, and each bacterium can secrete hormone, enzyme necessary to various crop, and collaboration is made
With, facilitate stimulate plant growth, improve utilization rate of fertilizer.The present invention is not particularly limited the source of each strain, this field
Conventional commercial strain.In an embodiment of the present invention, the strain is the original bacteria bought from the Ministry of Agriculture's strain library
Kind freeze-dried powder.The present invention is not particularly limited the activation of the original strain freeze-dried powder and the numerous mode of expansion, normal using this field
Rule operation.
Complex microorganism liquid bacterial agent of the present invention includes brown sugar, glucose, peptone, yeast extract, water and compound bacteria.
In the present invention, by weight, the dosage of the brown sugar is preferably 24~40 parts to each component, and more preferably 28~36 parts, most
Preferably 32 parts；The dosage of the glucose is preferably 2~6 parts, and more preferably 4 parts；The dosage of the peptone is preferably 1~
3 parts, more preferably 2 parts；The dosage of the yeast extract is preferably 1~3 part, and more preferably 2 parts；The dosage of the compound bacteria is preferred
It is 75~125 parts, more preferably 95~105 parts, most preferably 100 parts；The dosage of the water is preferably 1500~2200 parts, more
Preferably 1700~2000 parts, most preferably 1860 parts.
It in the present invention, include brown sugar, glucose, peptone and yeast extract in the complex microorganism liquid bacterial agent.One
Aspect, these groups are divided into compound bacteria and provide carbon source, nitrogen source and growth elements abundant, microorganism can be made preferably to breed；
On the other hand, these components provide large amount of organic again for crop, are conducive to the content for increasing crop chlorophyll.Except this it
Outside, the saccharomycete in compound bacteria cooperates organic matter abundant to be conducive to improve soil aggregate structure, makes loosing soil ventilative, in turn
Be conducive to the breathing and growth of beneficial bacterium and crop root.Above-mentioned complex microorganism liquid bacterial agent is applied to wait improve by the present invention
Soil, compound bacteria are no longer influenced by soil acidity or alkalinity and the content of organic matter, and the nutriment that can be carried using microbial inoculum is quickly numerous
It grows, play a role.The present invention is not particularly limited the source of the brown sugar, glucose, peptone and yeast extract, and this field is normal
Advise commercial product.
The present invention provides above-mentioned complex microorganism liquid bacterial agents in improvement soil, resists corps diseases, promotion farming
Application in object maturation or raising crop yield.
In the present invention, the crops are preferably long bean, asparagus or grape.The disease preferably includes yellow leaf, fruit
Paint face, root knot nematode disease, rotten, dead, one of root-rot and crown gall or a variety of.
In the present invention, above-mentioned complex microorganism liquid bacterial agent is preferably administered to wait improve or have crops by the application
In the soil of disease.For the present invention when applying above-mentioned complex microorganism liquid bacterial agent, each amount of application is preferably 5~20 public affairs
Pound/acre, more preferably 10~15 kgs/acre.Heretofore described application preferably punching is applied.The complex microorganism liquid bacterial agent
Application temperature be preferably 8~35 DEG C, more preferably 15~30 DEG C.The Dressing date of the complex microorganism liquid bacterial agent is preferred
To before fruit maturation after crop plant field planting.The frequency of administration of the complex microorganism liquid bacterial agent is preferably 20~30 days
Once, more preferably 25 days it is primary.
A kind of compound bacteria provided by the invention, complex microorganism liquid bacterial agent and its application are carried out below with reference to embodiment
Detailed description, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Original strain freeze-dried powder (bacillus subtilis, Lactobacillus rhamnosus, saccharomycete are bought from the Ministry of Agriculture's strain library
And photosynthetic bacteria), strain recovery expands culture.
Expand culture concrete operations: (1500L pure water is added, temperature rises to 50 DEG C or more in medium sterilization in 2000L tank
When in the case of stirring by fermentation tank culture medium be added tank in, after stirring, start to sterilize, steam is heated to
118 DEG C sterilize 30 minutes, from the pH value of sample tap sample detection culture medium after having sterilized.PH value is adjusted to 6.0 hereinafter, aseptic area connects
Kind (utilizes cooling water circulation, when tank temperature degree is down to 35 DEG C, be inoculated with using flame inoculation method), anaerobic fermentation (cultivation temperature 35
~37 DEG C, tank pressure is maintained at 0.05Mpa, and every 4h opens stirring once, stirs 5min, 100r/min, incubation time 48 every time
~72h).
According to Lactobacillus rhamnosus: bacillus subtilis: saccharomycete: the effective viable bacteria ratio of photosynthetic bacteria=8:4:2:4,
Four kinds are mixed through expanding micro- raw bacterium that culture obtains, obtains compound bacteria.
After measured, in compound bacteria each strain content are as follows:
Lactobacillus rhamnosus living bacteria count is 2 × 108cuf/g。
Bacillus subtilis living bacteria count is 1 × 108cuf/g。
Saccharomycete living bacteria count is 0.5 × 108cuf/g。
Photosynthetic bacteria living bacteria count is 1 × 108cuf/g。
Embodiment 2
Pure water 1860kg, brown sugar 32kg, peptone 2kg are weighed, glucose 4kg, yeast extract 2kg are multiple described in embodiment 1
Combined bacteria 100kg.Mixing, obtains complex microorganism liquid bacterial agent.
Embodiment 3
Weigh pure water 1766kg, brown sugar 28kg, peptone 1.5kg, glucose 3kg, yeast extract 1.5kg, 1 institute of embodiment
State compound bacteria 100kg.Mixing, obtains complex microorganism liquid bacterial agent.
Embodiment 4
Pure water 1876kg, brown sugar 34kg, peptone 3kg are weighed, glucose 4kg, yeast extract 3kg are multiple described in embodiment 1
Combined bacteria 80kg.Mixing, obtains complex microorganism liquid bacterial agent.
Embodiment 5
Experiment place is Henan Province Ningling County, and experimental period is in March, 2017, and experimental group is compound micro- described in embodiment 2
Biofluid microbial inoculum is rushed on grape and applied: primary before rudiment, first fruiting period is primary, and fruit expanding period is primary, and punching applies 10 kilograms every time
Complex microorganism liquid bacterial agent and 10 kilograms of Water soluble fertilizers (Soviet Union's rice a great number of elements Water soluble fertilizer, 5 kilograms/bag；Article No.: 2033)；Control
Group uses bacillus subtilis bacterium solution, and living bacteria count is 5 × 108Cuf/g, every time 10 kilograms and 10 kilograms of Water soluble fertilizers (Soviet Union's rice
A great number of elements Water soluble fertilizer, 5 kilograms/bag；Article No.: 2033) it, with experimental group same time carries out punching applies.
Experimental result:
(1) colouring of experimental group fruit is well-balanced, and color is bright；Control color obfuscation.Experimental group is improved compared with control group glossiness
50%-90%.
(2) the experimental group fruit maturation time is more early, and July 10 was started selling；Control group fruit maturation is later, July 25
It starts selling.
(3) sugar content of experimental group fruit is 22%；Control group at the same time the phase the sugar content of fruit be 15.2%.
(4) after picking fruit, storage is protected from light under room temperature, experimental group fruit can extend 3 compared with the storage time of control group fruit
It.
(5) experimental group fruit pure in mouth feel is sweet；Control group fruit mouthfeel is turned sour astringent.Experimental group is commented compared with control group mouthfeel
Divide and improves 50%~90%.
(6) experimental group plant disease resistance is good, and with spraing 3 medicines in one season of fungicide, and plant growing way is healthy and strong, root system hair
White, blade thickness leaf color is vivid.Control group plant growing way is weak, and disease resistance is poor, is gone and buy Chinese medicine 5 times with one season of fungicide, but leaf spot lesion
It is more, root system jaundice, the thin small darker in color of blade.
(7) average quality of experimental group single mature grape is 12 grams, and the average quality of control group single mature grape is
11.5 grams.From the point of view of overall yield, under same cultivated area, experimental group improves 40%-70% than the fruit gross mass of control group.
Embodiment 6
Experiment place is Shen county of Shandong, and experimental material is yellow, and color is light, the thin long bean of leaf.Experimental group is described in embodiment 2
The punching of complex microorganism liquid bacterial agent is applied, and punching applies period as long bean growth medium, and the punching amount of applying is 8 kilograms per acre.Control group does not rush
Apply processing.
Experimental result: experimental group long bean yellow blade switchs to green, and plant top grows young leaves again；Long bean is individually average
Length is 35cm, 5~7 centimetres longer than compareing；10 plants of long beans are respectively selected at random from experimental group and control group, what experimental group was grown
Long bean fruit sum is 1686, and the long bean fruit number that control group is grown is 830, and experimental group increases compared with control group fruit number
1 times.From the point of view of overall yield, under same cultivated area, experimental group is than control group volume increase 30~50%.
Embodiment 7
Experiment place is Henan Province Puyang County doctor township, and experimental period is on May 20th, 2017, and experiment crop is sponge gourd
(three mu of greenhouses, the ground are planted sponge gourd 3 years, after having root-knot nematode generation, are destroyed the root system of plant, are caused the plant cannot be more
Good absorption nutrition, so sponge gourd is not grown).1 mu of experimental group land used, remaining as control land used；Experimental group is multiple described in embodiment 3
It closes the punching of microbial liquid microbial inoculum to apply, 16 kilograms per acre, control group is not dealt with.After 10 days, sponge gourd growth conditions: experimental group are observed
Start growth, result；Root system observation is pulled up, experimental group plant root grows much white again, can absorb nutrition.And it compares
Group sponge gourd does not prick new root.
After 30 days, two mu of control lands used are divided into two groups of A, B, complex microorganism liquid bacteria described in embodiment 3 is applied in the punching of A group
Agent, 8 kilograms per acre, monthly punching is applied primary.Finally, A group sponge gourd can normal outcome.B group sponge gourd is not dealt with still, takes result to
Phase, sponge gourd result is latter to enter the i.e. generation death of expanding stage plant, cannot result.Observation when finally pulling up seedlings: used 3 institute of embodiment
It states the plot nematode that the punching of complex microorganism liquid bacterial agent is applied to occur less, unused place nematode completely is all.The description of test, this
Inventing the complex microorganism liquid bacterial agent can stimulate crop to take root and have inhibiting effect to root-knot nematode.
Embodiment 8
Experiment place is the Henan Province Puyang County town Wang Zhu, and experimental period is in June, 2017, the asparagus gross area of practice ground
It is 100 mu, practice ground is 10 mu, remaining as control land used；The punching of experimental group complex microorganism liquid bacterial agent described in embodiment 4
It applies, 16 kilograms per acre；Control group is not dealt with.
When complex microorganism liquid bacterial agent is applied in punching, asparagus is in and stays the stem phase experimental group.Experimental group asparagus is whole before punching is applied
Body root rot is serious, and dead rate reaches 50%.Experimental group does not make other any processing after punching is applied, and is observed within the 16th day, real
(Fig. 1-A) newly sends out asparagus seedling and with comparing control group asparagus (Fig. 1-B) significantly increases with testing group asparagus.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of complex microorganism liquid bacterial agent, which is characterized in that the component including following mass parts: 24~40 portions of brown sugar, 2~
6 parts of glucose, 1~3 part of peptone, 1~3 part of yeast extract, 75~125 parts of compound bacterias and 1500~2200 parts of water；It is described compound
Bacterium bag includes Lactobacillus rhamnosus, bacillus subtilis, saccharomycete and photosynthetic bacteria, and effective viable bacteria total amount in the compound bacteria >=
1×108CFU/g。
2. complex microorganism liquid bacterial agent according to claim 1, which is characterized in that in the compound bacteria, the mouse
Lee's sugar lactobacillus, bacillus subtilis, saccharomycete and effective viable bacteria ratio of photosynthetic bacteria are 6~10:2~6:1~3:2~6.
3. complex microorganism liquid bacterial agent according to claim 1, which is characterized in that in the compound bacteria, the mouse
The living bacteria count of Lee's sugar lactobacillus is 1~3 × 108CFU/g；The living bacteria count of the bacillus subtilis is 0.5~1.5
×108CFU/g；The living bacteria count of the saccharomycete is 0.25~0.75 × 108CFU/g；Effective viable bacteria of the photosynthetic bacteria
Number is 0.5~1.5 × 108CFU/g。
4. complex microorganism liquid bacterial agent according to claim 1, which is characterized in that the component including following mass parts:
32 portions of brown sugar, 4 parts of glucose, 2 parts of peptones, 2 parts of yeast extracts, 100 parts of compound bacterias and 1860 parts of water.
5. any one of Claims 1 to 4 complex microorganism liquid bacterial agent is in improvement soil, resistance corps diseases, promotion
Application in crop maturity or raising crop yield.
6. application according to claim 5, which is characterized in that the corps diseases include yellow leaf, fruit paint face, root knot
Any one or more in nematodiasis, rotten, dead, root-rot and crown gall.
7. application according to claim 5 or 6, which is characterized in that the application is by the complex microorganism liquid bacteria
Agent is applied to soil with 5~20kg/ mus every time of amount.
8. application according to claim 7, which is characterized in that the application temperature of the complex microorganism liquid bacterial agent is 8
~35 DEG C.
9. application according to claim 7, which is characterized in that the Dressing date of the complex microorganism liquid bacterial agent is agriculture
To before fruit maturation after crop plant field planting.
10. application according to claim 7, which is characterized in that the frequency of administration of the complex microorganism liquid bacterial agent is
20~30 days primary.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111567261A (en) * | 2020-05-14 | 2020-08-25 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN112574930A (en) * | 2021-01-15 | 2021-03-30 | 济南达信生物科技有限公司 | Lactobacillus rhamnosus and application thereof in preventing and treating plant root-knot nematodes |
| CN114634903A (en) * | 2022-05-05 | 2022-06-17 | 湖州师范学院 | Directional activation method of beneficial bacteria in soil and application of yeast lysate in growth promotion of lactic acid bacteria |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111567261A (en) * | 2020-05-14 | 2020-08-25 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN111567261B (en) * | 2020-05-14 | 2022-03-04 | 中国科学院沈阳应用生态研究所 | Plant toner and application and use method thereof |
| CN112574930A (en) * | 2021-01-15 | 2021-03-30 | 济南达信生物科技有限公司 | Lactobacillus rhamnosus and application thereof in preventing and treating plant root-knot nematodes |
| CN114634903A (en) * | 2022-05-05 | 2022-06-17 | 湖州师范学院 | Directional activation method of beneficial bacteria in soil and application of yeast lysate in growth promotion of lactic acid bacteria |
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