CN106699423A - Lycium barbarum root rot biocontrol preparation and preparation method thereof - Google Patents

Lycium barbarum root rot biocontrol preparation and preparation method thereof Download PDF

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CN106699423A
CN106699423A CN201710111500.6A CN201710111500A CN106699423A CN 106699423 A CN106699423 A CN 106699423A CN 201710111500 A CN201710111500 A CN 201710111500A CN 106699423 A CN106699423 A CN 106699423A
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bacteria
parts
matrimony vine
root rot
biological prevention
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张汉武
吕志刚
陶桂霞
马世清
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张汉武
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

Abstract

The invention relates to a Lycium barbarum root rot biocontrol preparation and a preparation method thereof. The Lycium barbarum root rot biocontrol preparation is composed of Streptomyces pactum in actinomycetes, photosynthetic bacteria, saccharomycetes, lactobacillus, Bacillus subtilis, phosphate-solubilizing bacteria, cellulase, humic acid, attapulgite and other beneficial microbial florae, and various organic acid plant growth regulators and other metabolites. Lycium barbarum root rot biocontrol preparation is not antagonistic with multiple strains and related enzymes, and can be compounded with the strains and related enzymes under synergic actions to prepare a composite microbial preparation mixture for preventing Lycium barbarum root rot. At the ambient temperature of 20-34 DEG C, the microbial preparation can quickly grow and reproduce in the humic acid and attapulgite environment, thereby increasing the quantity of soil actinomycetes, inhibiting the quantity of harmful fusarium, and achieving the balance of the Lycium barbarum growth environment and microbial environment; and thus, the Lycium barbarum root is free of interference of fusarium and other harmful bacteria and can grow healthily. The attapulgite provides 19 microelements, and achieves the microbial balance of the Lycium barbarum growth soil environment.

Description

A kind of matrimony vine root rot biological prevention and control agent and preparation method
Technical field
Biological field, more particularly to a kind of matrimony vine root rot biological prevention and control agent and preparation are prevented and treated the present invention relates to matrimony vine root rot Method.
Background technology
Qinghai Province is one of national tunnel Chinese medicine important locality, with advantageous natural resource advantages and a fixed output quota Industry development foundation.Matrimony vine is the traditional rare traditional Chinese medicine with help function of China.
Qinghai matrimony vine is planted in the growth of the Tibetan Plateau in one of more than 2000-3000 meters of big ultra-clean area of the world four, plantation Ground height above sea level, temperature is low, sunshine is long, Climate Dryness, light radiation are strong, day and night temperature is big, and plant photosynthetic efficiency is high, and night consumption is lacked, Growth period rain heat same season, relative air humidity is low, without high temperature influence, improves the accumulation of organic substance.Growing area away from cities and towns, Industrial and mining area, production environment is clean, and water body, soil, air are not polluted, and planting area is relatively independent, establishes one's own system, it is to avoid The harm that pest and disease damage is mutually propagated, produces the basic condition of organic matrimony vine preferably, has reached international counterparts industry standard.Matrimony vine resists Drought, salt tolerant domain are preferably economical and ecological and use seeds, can consolidate oasis, improve the overall ecology of sandy land and basin Environment, improves city dweller's production and living conditions, can be grown in sand ground, therefore can be as the shrub of water and soil conservation, in desertification Preventing and treating aspect effect is protruded.
Matrimony vine is that China's tradition has the rare traditional Chinese medicine with help function, and modern medicine study shows that matrimony vine has tonifying liver Kidney-nourishing, regulation body's immunity, can effectively resist tumour growth and red blood cell mutation, with anti-aging, AFL, tune The effect such as section blood fat and blood sugar, promotion hematopoiesis function.
Chaidamu Basin, Qinghai Province Chinese wolfberry fruit particle is big, full, the small plumpness of meat core, and sugared part is more and sweet, color and luster it is fresh and It is beautiful, be conducive to the matrimony vine flavones and LBP-X of health as many as to be doubled than allopatric species growing area.With outer producing region ratio, our province The tribute fruit level of matrimony vine 180-220/50g accounts for more than 70% (outer producing region only accounts for 35%), and the super large of wherein 100-130/50g is really Account for 15% or so in (outer producing region without super large really).Total sugar content is up to 53.6%-59.6%, and lycium barbarum total sugar content is than will It is low, it is 46.5%.Containing flavones 30%, total reducing sugar 12%, polysaccharide 10%, amino acid 9.08%, iron 16%, zinc 30%.Black fruit fructus lycii Containing 17 kinds of amino acid, 13 kinds of trace elements, wherein calcium, magnesium, copper, zinc, the content of iron are also far above lycium barbarum.Chinese Academy of Sciences northwest Plateau biological study to Qinghai Delingha District and Zhongweiof Ningxia, northwest China area matrimony vine sample determine, our province Chinese-wolfberry nutritive composition, Vitamin, mineral matter, amino acid equal size are substantially better than lycium barbarum.
In China, Qinghai matrimony vine cultivated area has occupied the whole nation second to world's matrimony vine plantation 95%, to the year two thousand twenty Qinghai matrimony vine Be the first in the nation for jump up to 600,000 mu by cultivated area.2012, Qinghai Nuo Muhong farms were regarded as by Guiness World Records " world height above sea level highest in flakes matrimony vine planting base ".Wood flood in the oasis in the Caidamu Basin-promise is also widely distributed to be can be rated as so far Domestic age of tree wild matrimony vine woodlot most long, there is 6 kinds of Lycium kinds such as black fruit fructus lycii, in the distribution of Dulan County oolong ditch There is the natural Chinese wolfberry fruit group that the state's inner area for finding so far is maximum, concentrate the most, these precious natural Chinese wolfberry fruit germ plasm resources are Matrimony vine rearing new variety provides abundant gene.
However, matrimony vine plantation at present there is problems:
Matrimony vine root rot occurs generally, and harm is serious, and Died Of Disease plant is annual in 2%-6%, and the morbidity of most important place reaches 40%, the death rate causes very big loss up to 25% to matrimony vine production.
Root-rot disease symptoms have following two types:
The rotten type of root.There is rotten, peeling phenomenon in various degree in root or root neck, stalk vascular bundle becomes brown, when moist Sick minister goes out white or pink mould layer.It can be divided to two kinds of microphyll type and yellow type again.
1. microphyll type:In evening lamina time in spring, leaf is small, branch is downgraded, bud and fruit are modest, and normal bud drop is full when serious Strain is withered.
2. yellow type:Yellowing leaf, has wilting and phenomenon of not wilting, and Chang great Liang fallen leaves are seriously withered to complete stool, also have Germinateed new leaves again after leaf, it is repeated multiple times rear withered.
Rot type.The cortex of root neck or branch stalk becomes brown or black rots, and vascular bundle is changed into brown.It is yellow when blade tip starts Color, gradually dried-up, upward warp, when rotten cortex is around trunk, sick portion's above leaf comes off entirely, and trunk is withered, and what is had is then Blade is wilted suddenly withered, and dead leaf still hangs on the tree.This phenomenon mostly occurs in the high temperature season in 7-8 months.
Above-mentioned all kinds, also often show as the half of withered or only branch morbidity of half of tree crown morbidity and wither.Have Trunk is died of illness, and is grown new tiller in tree root neck and given birth to seedling.
2nd, pathogenic factor:
Pathogen survives the winter with survival diseased plant, also can survive the winter and propagate with the diseased plant residuum in table soil and soil and disease fruit seed. Germ directly intrudes into inside plant tissues from wound or through tissue divots, causes morbidity.Different pathogen and different Dip-dye mode, the incubation period of its disease is also different.Under the conditions of 20 DEG C, Fusarium oxysporum causes under conditions of host's wound 3 days sick incubation periods, and when not injured it is 19 days, eggplant V. albo-altrum is followed successively by 5 days and 19 days;Fusarium concolor is in host's wound bar Caused a disease under part 5 days incubation periods.Field ponding is the major reason for increasing the incidence of disease.The poor soil of the aeration sand better than aeration Loam increases 9%-22.5%;Intertillage operation causes root to damage the invasion for being conducive to pathogen.
3rd, matrimony vine root rot
First, pathogen
Matrimony vine (Lyeium barbarum L.) root rot is a kind of system infections disease, and diseased plant root and root neck occur rotten Rotten, cane vascular bundle becomes brown.Disease portion grows white or pink mould layer (pathogen) once in a while when moist.Due to root-rot, blade Diminish, lack gloss, branch is downgraded, fruit slight of stature;Or blade flavescence is wilted, plant is gradually withered when serious.Some diseased plant roots Neck becomes brown with the cortex of limb, rots, and blade tip turns yellow, and crispaturas upwards, gradually shrivelled.When rotten cortex is around trunk, disease Portion's above trunk is withered.Some branches and leaves are wilted dry up suddenly, and dead leaf still hangs on the tree.Matrimony vine root rot the main pathogenic fungi is 3 kinds Sickle-like bacteria, (fusarium acuminatum Fusarium acuminatum, fusarium moniliforme F.monilifome Sheldon and sharp spore sickle Knife bacterium F.OxysporumSchlecht).
Virulent pathogen can directly invade from wound or epidermis, and the weak pathogen of pathogenicity enters from wound more Invade.
The generation of matrimony vine root rot is proportionate with epidemic disaster.General temperature is higher, humidity is bigger, falls ill heavier.It is of that month Temperature on average at 21-26 DEG C, when more than more than 78% easily fall ill by field relative humidity.Matrimony vine different cultivars is to root rot There were significant differences for resistance.
A kind of continuous and effective is provided and the good matrimony vine root rot pesticide control of prevention effect just seems particularly necessary.
The content of the invention
To solve the problems, such as above-mentioned prior art, it is an object of the invention to a kind of matrimony vine root rot biological prevention and control agent and Preparation method, the component of the matrimony vine root rot biological prevention and control agent by:Close rotation streptomycete in actinomyces, photosynthetic bacteria, saccharomycete, breast Beneficial microbe colonies such as sour bacterium, bacillus subtilis, phosphate solubilizing bacteria, cellulase activity, humic acid, concave convex rod and various organic The metabolites such as acid plant growth regulator.And with various bacterial strains and relevant enzymes mutually not antagonism, mutually cooperate with and compounds and be made A kind of complex microorganism preparations mixture for preventing matrimony vine root rot.At a temperature of environment 20-34 degree, microorganism formulation is in corruption Grow acid and concave convex rod environment can fast-growth with breed, increased the quantity of soil actinomycete, it is suppressed that harmful bacteria sickle-like bacteria Quantity, reached the balance of the microbial environment of matrimony vine growing environment, make matrimony vine root not by the harmful bacteria such as reaping hook mycocriny Disturb and healthy growth, while providing 19 kinds of trace elements, reach the balance of matrimony vine soil for growth environmental microorganism, serve Rehabilitating soil, the purpose for improving yield, while saccharomycete, cellulase, phosphate solubilizing bacteria can decompose debirs, so as in soil Accelerate the decomposition of organic matter, carbon, nitrogen, phosphorus, potassium, sulphur etc. are decomposed, by high molecular weight protein and long-term use Diammonium phosphate (DAP) in soil The insoluble phosphate of middle formation is converted into the crop easily small molecular protein for utilizing and the phosphate that can be dissolved, and is conducive to Chinese holly Qi absorbs, so as to strengthen the resistance against diseases of crop, the effect of some functional microorganisms and trace element contained therein, to matrimony vine Growth is highly advantageous.
To reach above-mentioned purpose, the technical scheme is that:
A kind of matrimony vine root rot biological prevention and control agent, the component of the matrimony vine root rot biological prevention and control agent presses mass fraction by close rotation strepto- 60-70 parts of bacterium active bacteria formulation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, bacillus subtilis 5-15 parts, lactic acid bacteria 6-10 parts, Yeast strain 3-7 parts, cellulase activity 1-3 parts of composition.
A kind of matrimony vine root rot biological prevention and control agent, the relevant art index of each component is:
A kind of preparation method of matrimony vine root rot biological prevention and control agent, the preparation method of the matrimony vine root rot biological prevention and control agent is by following Step is constituted:
Step one, by it is close rotation 60-70 parts of streptomycete active bacteria formulation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, withered grass bud 5-15 parts of spore bacillus, lactic acid bacteria 6-10 parts, yeast strain 3-7 parts, cellulase activity 1-3 parts, be put into after weighing in mixer and stirred Mix uniform, obtain bacteria agent;
Step 2, bacteria agent is mixed in proportion with organic fertilizer:
Bacteria agent:The ratio of organic fertilizer is 1:1~100, fully mix and just obtain boxthorn root maize ear rot biological prevention and control agent.
A kind of preparation method of matrimony vine root rot biological prevention and control agent, in the step 2, organic fertilizer is well-rotted farmyard manure:Humic Acid:Concave convex rod in mass ratio 7:2:1 is mixed to get.
Further, application of the matrimony vine root rot biological prevention and control agent on rehabilitating soil.
Further, application of the matrimony vine root rot biological prevention and control agent in the anti-root rot ability of enhancing crop.
Relative to prior art, beneficial effects of the present invention are:
A kind of matrimony vine root rot biological prevention and control agent, it is characterised in that the component of the matrimony vine root rot biological prevention and control agent by:Actinomyces In close rotation streptomycete, photosynthetic bacteria, saccharomycete, lactic acid bacteria, bacillus subtilis, phosphate solubilizing bacteria, cellulase activity, humic acid, The metabolite such as the beneficial microbe colonies such as concave convex rod and various organic acid plant growth regulators.And with various bacterial strains and phase Close enzyme mutually not antagonism, mutually collaboration compounding and a kind of complex microorganism preparations mixture for preventing matrimony vine root rot for being made. At a temperature of environment 20-34 degree, microorganism formulation humic acid and concave convex rod environment can fast-growth and breeding, increased soil The quantity of earth actinomyces, it is suppressed that the quantity of harmful bacteria sickle-like bacteria, has reached the balance of the microbial environment of matrimony vine growing environment, Make matrimony vine root by the harmful bacteria such as reaping hook mycocriny interference and healthy growth, while providing 19 kinds of trace elements, reach Chinese holly The balance of Qi soil for growth environmental microorganism, serve rehabilitating soil, improve yield purpose, while saccharomycete, cellulase, Phosphate solubilizing bacteria can decompose debirs, so as to accelerate the decomposition of organic matter in soil, carbon, nitrogen, phosphorus, potassium, sulphur etc. be decomposed, will be big Molecule protein is converted into small point that crop easily utilizes with the insoluble phosphate that long-term use Diammonium phosphate (DAP) is formed in soil Sub- albumen and the phosphate that can be dissolved, are conducive to matrimony vine to absorb, so as to strengthen the resistance against diseases of crop, some contained therein The effect of functional microorganism and trace element, grows highly advantageous to matrimony vine.
Specific embodiment
Present invention is described in further detail with reference to specific embodiment:
A kind of matrimony vine root rot biological prevention and control agent, the component of the matrimony vine root rot biological prevention and control agent presses mass fraction by close rotation strepto- 60-70 parts of bacterium active bacteria formulation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, bacillus subtilis 5-15 parts, lactic acid bacteria 6-10 parts, Yeast strain 3-7 parts, cellulase activity 1-3 parts of composition.
A kind of matrimony vine root rot biological prevention and control agent, the relevant art index of each component is:
A kind of preparation method of matrimony vine root rot biological prevention and control agent, the preparation method of the matrimony vine root rot biological prevention and control agent is by following Step is constituted:
Step one, by it is close rotation 60-70 parts of streptomycete active bacteria formulation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, withered grass bud 5-15 parts of spore bacillus, lactic acid bacteria 6-10 parts, yeast strain 3-7 parts, cellulase activity 1-3 parts, be put into after weighing in mixer and stirred Mix uniform, obtain bacteria agent;
Step 2, bacteria agent is mixed in proportion with organic fertilizer:
Bacteria agent:The ratio of organic fertilizer is 1:1~100, fully mix and just obtain boxthorn root maize ear rot biological prevention and control agent.
A kind of preparation method of matrimony vine root rot biological prevention and control agent, in the step 2, organic fertilizer is well-rotted farmyard manure:Humic Acid:Concave convex rod in mass ratio 7:2:1 is mixed to get.
Application of the matrimony vine root rot biological prevention and control agent on rehabilitating soil.
Application of the matrimony vine root rot biological prevention and control agent in the anti-root rot ability of enhancing crop.
Experimental example:
If control, conidia powder are mixed the inoculation of humic acid root dipping, organic fertilizer and be inoculated with 3 treatment, each treatment is repeated 2 times.
Conidia powder mixes humic acid inoculation:Bacterium amount is connect for 1.5g/kg, spore amount is 8,000,000,000.
Root dipping is inoculated with:By actinospore powder and humic acid in mass ratio 1:9 mix, and add water 10-15kg, matrimony vine root It is placed at lucifuge after dipping in, bacterium powder is uniformly wrapped up general planting after matrimony vine seedling appearance root.The average inoculating spores amount of each matrimony vine seedling It is 400,000,000, measurement average value before spending, the florescence for the treatment of, the root system for the treatment of increased than control length than control 6-7 days in advance 19%.Radical amount increases by 21.6%, year-end units increased in production 19.8%.
With reference to influence of the biocontrol microorganisms to plant strain growth, to application principle of the invention, the present invention is further described.
Organic fertilizer is inoculated with:1 is by the biological prevention and control agent of the invention:By 1000 grams+organic fertilizer, (wherein organic matter is more than 40% 66kg or well-rotted farmyard manure 1000kg), stir, when First Year is sowed with organic fertilizer with base manure form uniformly spread fertilizer over the fields in Ground, Second Year, life in the 3rd year with reference to tillage and weeding and are topdressed, and will be prepared bacterial manure and are applied in ditch spread mode, and 2 are:By 1000 grams (wherein organic matter is more than 40%66kg or well-rotted farmyard manure to the preventing and treating matrimony vine root rot biological agent+organic fertilizer for preparing 1000kg, stirs, and uniformly spreads fertilizer over the fields in 666.7 square metres of Tanaka, and every square metre of microbial bacteria dosage for applying is 8,100,000,000, Because the rational density of matrimony vine is 333 plants/acre, every plant accounts for 2 square metres of areas, and every plant of average beneficial bacterium bacterium amount that possesses is 10,000,000,000, Saccharomycete, lactic acid bacteria, photosynthetic bacteria, cellulose enzyme activity decompose organic matter in medlar field, and the effect of energy is provided for actinomyces Under in Tanaka's field planting, being capable of fast-growth and breeding under the conditions of suitable (temperature, humidity), it is suppressed that the quantity of harmful bacteria, reach The balance of the microorganism arrived in matrimony vine growing environment, make matrimony vine root by the harmful bacteria such as fungi, sickle-like bacteria interference without Balance that is sick, reaching matrimony vine growth microorganism, serves rehabilitating soil microorganism species, the purpose of yield is improved, while ferment The floras such as female bacterium can decompose debirs, so as to accelerate the decomposition of organic matter in soil, carbon, nitrogen, phosphorus, potassium, sulphur etc. be decomposed, High molecular weight protein is converted into the small molecular protein that crop easily utilizes, it is easy to which matrimony vine absorbs, so as to strengthen the disease-resistant energy of crop Power, because every square metre of beneficial bacterium increased 8,100,000,000, while applying 66kg humic acid or 1000 to be effectively prevented root (stem) rotten Disease, volume increase reaches 22.3%.
Compost:2 kilograms of biocontrol microorganisms are added in 1000 kilograms of oxen of becoming thoroughly decomposed, sheep manure organic fertilizer (water content 33%-40%) Mixing, and stir (stand-by).
Need further exist for explanation be:Biological prevention and control agent of the invention, can not be mixed with chemical bactericide to person poultry harmless, extremely Few time interval is used again after 48 hours.
Watered if soil moisture content is poor, after fertilising or be watered to pour and applied.Avoid exposure in sunshine.
Be in a bad way plot can appropriate escalated dose, additionally need explanation is all:This product can not be used instead of bactericide.
Actinomycetes strain used of the invention is the multi-functional actinomyces screened in the western extreme habitat of China, by Shaanxi poplar Ling Kang is provided according to agricultural development Co., Ltd.
Product active ingredient is streptomycete viable spore.It is the continuous cropping obstacle nitrate nitrogen contamination prepared by solid state fermentation Agent, bacterial strain uses therefor is the multi-functional actinomyces screened from the western extreme habitat of China.The bacterium can produce the specific crop soil of suppression Antibiotic, secretion plant hormone and the specific root of the crop of degrading for passing disease pathogen secrete suppression harmful bacteria, can effectively repair continuous cropping work The soil microenvironment that Wu Gen areas degenerate, suppresses growth of pathogenic bacteria, reduces or eliminates root and secretes suppression harmful bacteria, promotes plant growth Development.This product has growth-promoting diseases prevention dual-use function, is mainly used in the microorganism remediation of garden crop continuous cropping obstacle, to applicable crops Soil-borne disease pathogen has obligate inhibitory action, can have matrimony vine to plant (continuous cropping) soil-borne disease, and enhancing plant root and blade are protected Shield property enzymatic activity, stimulates root growth, promotes absorption of the crop to nutrient and water, improves yield, improves quality, promotes precocious.
The active ingredient of microbial inoculum is actinomyces viable bacteria spore.Microbial inoculum is water insoluble, with root system surrounding soil directly contact Can play a role.
Microbial inoculum has micro organism quantity species effect in adjustment root system surrounding soil, can suppress harmful bacteria growing, and increase has Beneficial bacteria quantity, mitigates soil-borne disease and continuous cropping obstacle, stimulates root system development, promotes plant growth, improves yield.
Microbial inoculum should not be administered alone, it is necessary to the cake powder such as high-quality organic fertilizer, the rape of abundant fermentation maturity or humic acid, etc. Fertilizer is applied, and synchronously applies NPK composite fertilizers effect more preferable.
Mixed proportion, microbial inoculum:Organic fertilizer=1~100, fully mix, and spread manuer in holes, every plant of 650--1000 grams of left side of 4 years raw matrimony vines It is right.
Microbial inoculum mix with organic fertilizer using have be mutually improved effect:Organic fertilizer provides nutriment for actinomyces, promotes Actinomyces breed in soil, and bacterium number is higher, and effect is better;Decomposition of the actinomyces to organic fertilizer has a facilitation, formation it is small Molecular organic has facilitation to plant growth;Microbial inoculum stimulates root system development, when synchronously applying N, P, K, due to crop root Prosperity, can promote crop to N, P, K for being applied in soil and the Utilization ability of moisture.
Microbial inoculum is specially with the matching method of organic fertilizer:
Microbial inoculum presses 1 before use with organic fertilizer:100 ratios are fully mixed.
Method:Microbial inoculum:Organic fertilizer=1:1 expands step by step, it is impossible to during a small amount of microbial inoculum is once added a large amount of organic fertilizers, so Mix irregular impact effect.
For example:200g microbial inoculum+200g organic fertilizers, fully mix, and obtain 400g bacterial manure mixtures;400g mixtures+400g Organic fertilizer, fully mixes, and obtains 800g bacterial manure mixtures;800g bacterial manure mixture+800g organic fertilizers, fully mix, and obtain 1600g bacterial manure mixtures, the like.Concrete operations so must carefully claim, but it should expanding step by step, be sure not once to add!And will The last ratio of control.Potassium humate class organic fertilizer effect is good,
Additionally need supplementary notes is:
Microbial inoculum can not replace normal fertilising, but can improve fertilizer and efficiency of water application by promoting root system development.
Microbial inoculum is more preferable with composite fertilizer and organic fertilizer compounding application effect;It is good in water and fertilizer condition and soil organic matter content is higher When, growth-promoting effect becomes apparent from.
Microbial inoculum can not simultaneously be used with bactericide.
Actinomyces are adapted to neutral and slightly alkaline soil.When being applied on acid soil, coordinate alkaline fertilizer, effect can be more It is good.
Matrimony vine root rot generally occurs have been carried out separating identification to its cause of disease, and common typically has 3 kinds of pathogens:It is sharp Push up sickle-like bacteria Fusarium acuminatum, fusarium moniliforme F.monilifomeSheldon and Fusarium oxysporum F.OxysporumSchlecht), activated in PDA culture medium standby.
The advantage unwrapping wire dientification of bacteria
Actinomyces are extracted using enzymatic isolation method, send Nanjing X bio tech ltd to be sequenced.The ferment filtrate bacteriostatic test bacterium It is that the advantage actinomyces for trying to be separated in healthy tree soil, identified from confession are close rotation streptomycete, with advantage actinomyces in inclined-plane culture 7d, Plus sterilized water 4mL, spore is scraped with bamboo stick, is stirred evenly, it is made spore suspension.Spore suspension 1mL is drawn with 1mL aseptic straws extremely In 250mL vials equipped with No. 1 fluid nutrient medium of 60mL Gao Shi, sealed with 4 layers of cotton, repeat 3 bottles.27 DEG C, 160 turns every point Shaking table shaken cultivation 7d after filtered with filter paper, 0.45 μm of miillpore filter vacuum filtration, 0.45 μm of sterilizing filtering with microporous membrane are removed Bacterium obtains ferment filtrate.To cultivated sickle-like bacteria, culture 7d) in add sterilized water 4mL, mycelia is scraped with bamboo stick, is stirred evenly.With 1mL aseptic straws draw 0.1mL bacteria suspensions in PDA bacteria suspensions in improved culture medium) on plate, smoothened with spatula.28 DEG C of cultures After 4d, 7mm circle bacteria cakes are made of card punch, it is standby.Actinomycete fermentation filtrate be cooled to 50 DEG C or so PDA (or improvement PDA) culture medium is down flat plate after 1 ︰ 4 is mixed by volume, and actinomycete fermentation filtrate as control is replaced with sterilized water.With sterilizing bamboo stick Picking pathogen agar block puts above-mentioned flat board center, and bacterium downwards, often processes 3 repetitions.27 DEG C of culture 60h, use right-angled intersection Method measurement colony diameter simultaneously calculate bacteriostasis rate, its result be actinomyces 5,400,000,000/gram.
Fusarium oxysporum F.OxysporumSchlecht, fusarium acuminatum Fusarium acuminatum, beading reaping hook Bacterium F.monilifome Sheldon and, activated in PDA culture medium standby.Wherein Fusarium oxysporum pathogenicity is most strong.To upper State the growth temperature of cause of disease, pH value has made indoor measurement this sick pathogen can survive the winter with strain, invalid body and disease fruit seed.Matrimony vine Different cultivars has notable difference to this sick resistance, and field ponding and root damage are beneficial to morbidity.Diseased plant root and root neck occur rotten Rotten, cane vascular bundle becomes brown, and disease portion grows white or pink mould layer (cause of disease seedling) once in a while when moist.Due to root-rot, Ye Shang Trunk is withered.Some branches and leaves are wilted dry up suddenly, and dead leaf still hangs on the tree.What above-mentioned symptom had on one tree shows as part Major branch is fallen ill, and some trunks can again send forth branches bar after dying of illness in root neck.GeneraI investigation matrimony vine root-rot summer in 2013 generally occurs, Most light diseased plant 0.8%, withered strain 0.3%;Most heavy diseased plant 40%, withered strain 25.5%;Average diseased plant l5%, withered 5%, It is that the major disease 2013-2016 author of matrimony vine one has made intensive studies with regard to the generation and preventing and treating of this disease, it is as a result as follows:
Materials and methods, Isolation and identification of pathogen gathers the rhizome and branch of different type diseased plant in Dulan county wolfberry orchard Bar, rinses well, after selecting diseased tissues sterilized, is separately cultured on potato sucrose agar (PDA) culture medium.Treat that spore grows Afterwards with " agar-agar planar surface monospore picking method carries out monospore separation and culture.Categorizing system is identified.Plant causing a disease for separator Property determine.
Strain separating thing is accessed sickle-like bacteria plant inoculating nutrient solution by the preparation for trying bacterium solution respectively, in 24 DEG C of incubators Culture 20 days, is made respective inoculation liquid after filtering.
For the examination potted plant disease-free matrimony vine seedling that grows directly from seeds of plant.
Inoculation method is tried in bacterium solution and control (clear water), and daytime, put in 24 DEG C of incubators in the evening at room temperature, is counted after 5 days Get a haircut everywhere diseased plant number.Bacterium solution root-pouring method:To be poured onto for examination bacterium solution and fill 200ml through hindering root and not hindering root, hair will be investigated after 20 days Diseased plant, records symptom, separates again and identifies.
Pathogen survive the winter and inspection (one) invalid body of route of transmission in pathogen survive the winter and checked invalid body in the l0 months (root, stem) is respectively placed in field soil table and is embedded in the deep soil of 10-20cm, and April in next year is separately cultured, inspection pathogen survival Situation.(2) seed and nursery stock carry disease germs inspection adopt disease fruit seed and randomly select seedling art sample strain, be separately cultured.
For examination bacterium solution matrimony vine pine root fungus Fusarium oxysporum and the equivalent mixed liquor of fusarium acuminatum.Inoculation and investigation, often Flowerpot is poured for examination bacterium solution 200ml, is inoculated with the 35th day and is investigated.
The potted plant inoculation test of relation 1. of root is hindered in morbidity with intertillage:If hindering root and not hindering two treatment of root, 2 basins 4 are often processed Strain, is repeated 4 times, 35 days investigation diseased plant rates after treatment;2. field test:The age of tree wolfberry orchard of 10 years is selected, it is severely injured if not hindering root Till (control) 3 is processed and observes accordingly result in root and routine.
The medium diseased plant of crop field biological agent pouring root experiment selection severity and bacteria agent, adjust for 40 days after liquid pouring root Look into prevention effect.As a result, matrimony vine root rot Species of Pathogens and its pathogenicity result show that matrimony vine Pathogens Causing Root Rot Disease is 3 kinds Typical sickle-like bacteria;
1 kilogram of function bacterium is added to 2000 kilograms of organic fertilizer (water content 33%- by biological prevention and control agent of the present invention in use 35%) mixing in, and it is stand-by to stir.
Microbial inoculum mix with organic fertilizer using have be mutually improved effect:Organic fertilizer provides nutriment for actinomyces, promotes Actinomyces breed in soil, and bacterium number is higher, and effect is better;Decomposition of the actinomyces to organic fertilizer has a facilitation, formation it is small Molecular organic has facilitation to plant growth;Microbial inoculum stimulates root system development, when synchronously applying NPK, due to crop root hair Reach, crop can be promoted to the NPK applied in soil and the Utilization ability of moisture.
Another object of the present invention is to photosynthetic bacteria, saccharomycete, lactic acid bacteria, bacillus subtilis, phosphate solubilizing bacteria, cellulose Enzyme activity belongs to autotrophy microorganism, and it can utilize the solar thermal energy of soil receiving or with ultraviolet as the energy, by soil Hydrogen in hydrogen sulfide and hydrocarbon is separated, become harmful substance be innocuous substance, and with the secretion of plant root, have Machine thing, pernicious gas (hydrogen sulfide etc.) and carbon dioxide, nitrogen etc. are matrix, synthesis carbohydrate, amino acid, vitamins, nitrogen Compound and physiological activator etc. are rich soils and promote the main force of animal and plant growth.The metabolite of photosynthetic bacteria or Person is directly absorbed by plant, the nutrient as other microbial reproductions, can breed photosynthetic bacteria, and other beneficial micro- lifes also can Propagation.For example:As bait, it can dissolve insoluble phosphorus to the amino acid that phosphorus decomposing VA Mycorrhizal Fungi is secreted with photosynthetic bacteria, but can and fixed nitrogen Bacterium symbiosis, significantly improves its nitrogen fixing capacity.
Another object of the present invention is to lactobacillus:Lactic acid bacteria (anaerobic type), it is produced with absorbing photosynthetic bacteria saccharomycete Based on the materials such as raw carbohydrate, lactic acid is produced.Lactic acid has very strong sterilizing ability, can effectively suppress harmful microbe work Move, and the drastically corrupt of organic matter is decomposed.Lactic acid bacteria can become lignin and cellulose for being not easily decomposed under normality etc. Easily decompose, and eliminate a variety of drawbacks that undecomposed organic matter is produced, the weight of shock brigade is played in organic matter fermentation decomposition Act on, the organic substance not become thoroughly decomposed is changed into the nutrient effective to animals and plants by it.Lactic acid bacteria can also effectively suppress continuous cropping barrier The pathogenic bacteria propagation of umbilical dystocia life.Pathogenic bacteria are enlivened, and harmful nematode can be sharply increased, and plant will be weak, and lactic acid bacteria inhibits cause Germ, harmful nematode will fade away.
The surface area of the influence attapulgite clay another object of the present invention is to concave convex rod to crops soil environment 140m2The perfect adaptation of/g concave convex rods and slow-release fertilizer, greatly alleviates infringement of the chemical fertilizer to soil, and farming is improved well The soil environment of thing growth, for the nutrient attapulgite that the growth of its crops provides abundance has very strong adsorptivity, water suction Property, catalytic and ion exchangeable, are in a kind of nonmetallic mineral resource for having very much use value.The unique thing of attapulgite Physical chemistry characteristic, can gather together with slow-release fertilizer well, can effectively improve the utilization rate of fertilizer, and can be its crop Grow beneficial Mg be provided2+、Si4+、Al3+、Fe3+Its content is SiO255.6-60.5;Al2O33 9.0-10.1; Fe2O35.7-6.7;Na2O0.03-0.11;K2O 0.96-1.30;CaO 0.42-1.95MgO 10.7-11.35;MnO 0.61; TiO2 0.32-0.6 trace elements.
Another object of the present invention is to humic acid inherently containing the elemental carbon needed for plant growth, hydrogen, oxygen, nitrogen etc., It can improve the utilization rate of nitrogen, phosphorus, potash fertilizer again simultaneously, so, humic acid is obviously for the effect of increasing production of crop.
Product principle active component is streptomycete viable spore.Be prepared by humic acid medium solid state fermentation and with light Close the collective effect matrimony vine root rot of bacterium, saccharomycete, lactic acid bacteria, bacillus subtilis, phosphate solubilizing bacteria, cellulase activity etc., connect Make obstacle micro-ecological repairing additive, bacterial strain uses therefor is the multi-functional actinomyces screened from the western extreme habitat of China.The bacterium can produce The raw antibiotic for suppressing specific soil-borne disease of crop pathogen, secretion plant hormone and specific root of the crop secretion from poisonous substance of degrading Matter, can effectively repair the soil microenvironment of succession crop root area degeneration, suppress growth of pathogenic bacteria, reduce or eliminate root secretion Suppression harmful bacteria is produced, promotes crop growth.This product has growth-promoting diseases prevention dual-use function, is mainly used in matrimony vine and makees root rot Microorganism remediation, has obligate inhibitory action to applicable crops soil-borne disease pathogen, can effectively prevent and control strawberry, watermelon, Muskmelon continuous cropping plants (continuous cropping) soil-borne disease, strengthens plant root and blade protection enzymatic activity, stimulates root growth, promotes to make Absorption of the thing to nutrient and water, improves yield, improves quality, promotes precocious.
After 750 kilograms of organic fertilizers uniformly can be spread fertilizer over the fields when planting then, cultivation matrimony vine seedling after rotary tillage, -3 years 1 year are raw every 300-550 grams of ditch spread of strain is spreaded manuer in holes, 4 years 650 grams or so of raw every plant of matrimony vines, and 1000 grams of every plant of matrimony vine adds nitrogen, phosphorus, potassium within more than 4 years.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any The change or replacement expected without creative work, should all be included within the scope of the present invention.Therefore, it is of the invention Protection domain should be determined by the scope of protection defined in the claims.

Claims (6)

1. a kind of matrimony vine root rot biological prevention and control agent, it is characterised in that the component of the matrimony vine root rot biological prevention and control agent presses mass fraction By close 60-70 parts of streptomycete active bacteria formulation of rotation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, bacillus subtilis 5-15 parts, breast Sour bacterium 6-10 parts, yeast strain 3-7 parts, cellulase activity 1-3 parts of composition.
2. a kind of matrimony vine root rot biological prevention and control agent according to claim 1, it is characterised in that the relevant art of each component refers to It is designated as:
3. a kind of preparation method of matrimony vine root rot biological prevention and control agent, it is characterised in that the preparation of the matrimony vine root rot biological prevention and control agent Method is made up of following steps:
Step one, by it is close rotation 60-70 parts of streptomycete active bacteria formulation, photosynthetic bacteria 5-15 parts, phosphate solubilizing bacteria 3-4 parts, bacillus subtilis Bacterium 5-15 parts, lactic acid bacteria 6-10 parts, yeast strain 3-7 parts, cellulase activity 1-3 parts, stirring is equal during mixer is put into after weighing It is even, obtain bacteria agent;
Step 2, bacteria agent is mixed in proportion with organic fertilizer:
Bacteria agent:The ratio of organic fertilizer is 1:1~100, fully mix and just obtain boxthorn root maize ear rot biological prevention and control agent.
4. a kind of preparation method of matrimony vine root rot biological prevention and control agent according to claim 3, it is characterised in that the step In two, organic fertilizer is well-rotted farmyard manure:Humic acid:Concave convex rod in mass ratio 7:2:1 is mixed to get.
5. application of any matrimony vine root rot biological prevention and control agents of claim 1-2 on rehabilitating soil.
6. application of any matrimony vine root rot biological prevention and control agents of claim 1-2 in the anti-root rot ability of enhancing crop.
CN201710111500.6A 2017-02-28 2017-02-28 Lycium barbarum root rot biocontrol preparation and preparation method thereof Pending CN106699423A (en)

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