CN109456105A - A kind of microbial bacterial agent method of preparation and use for alleviating solanaceous crops continuous cropping obstacle - Google Patents

Abstract

A kind of microbial bacterial agent method of preparation and use for alleviating solanaceous crops continuous cropping obstacle, it is related to the technical field of microbial bacterial agent.It is made of the raw material of following parts by weight: Methylotrophic bacillus, bacillus subtilis, bacillus licheniformis, colloid bacillus cereus, saccharomycete, photosynthetic bacteria, humic acid, potassium fulvate, potassium humate, amino acid fertilizer, potassium hydrogen phosphate, modified biomass charcoal, alginic acid, chitin, peptone, middle microelement.The invention has the following beneficial effects: base fertilizer application can only be done and colonize difficult disadvantage by making up common microbial bacterial agent, it can occur in the root rot that the breeding time of entire solanaceous crops all plays prevention continuous cropping obstacle, and it applies conveniently, the auxiliary material in microbial inoculum is conducive to colonizing and active enhancing for functional microorganism simultaneously, better effect is applied with the punching of solanaceous crops water-fertilizer integral, the generation for preferably preventing root rot in continuous cropping, promotes the stable high yield of solanaceous crops.

Description

A kind of microbial bacterial agent method of preparation and use for alleviating solanaceous crops continuous cropping obstacle

Technical field

The present invention relates to the technical field of microbial bacterial agent, a kind of microbial bacterial agent system for alleviating solanaceous crops continuous cropping obstacle Standby and application method.

Background technique

With the adjustment of agricultural structure, a large amount of country labor force transfers, planting industry is quickly sent out to scale is intensive Exhibition, a large amount of vegetable cultivation village specializing in a certain trade, professional rich and influential family, base, cooperative society and enterprise continue to bring out, and vegetable cultivation area expands rapidly Greatly.During vegetable cultivation, tree-like big due to solanaceous crops, as a result fecund amount is big, and harvest time is long, cultural method simply and It is with a long history, it is good in economic efficiency, it is deep to be liked by farmer.Therefore, solanaceous crops cultivated area accounting is larger.

In the cultivation of the solanaceous crops such as capsicum, eggplant and tomato, due to large-scale planting and experience is cultivated The long-term same crop of continuous cropping is accustomed in limitation, farmer or enterprise, causes solanaceous crops continuous cropping obstacle to increasingly sharpen, soil-borne disease Occur seriously, to increase Pesticide use amount, cause the residual of victual exceeded, and reduce quality of vegetable.

Functional microorganism in the microbial bacteria of presently commercially available alleviation continuous cropping obstacles is due to climate, soil characteristic And the influence of Tu microorganism, it colonizes all relatively difficult, causes effect that functional microorganism plays in continuous cropping soil just It is more small, so the alleviation continuous cropping obstacles played the role of are all unobvious.It is answered even if having and alleviating capsicum continuous cropping obstacle It closes microbial bacterial agent (application number 201510228810.7), the raw material of patent is with cow dung, honeycomb cinder, recessed soil due to can not It is water-soluble, applied in base fertilizer it is more convenient, it is just inconvenient in the generation that prevention root rot is applied in later period punching, and solanaceous crops connect Make the soil root rot high-incidence season occurred before the florescence and tail fruiting period all after transplanting, was prevention solanaceous crops root before the two periods The emphasis of maize ear rot.The present invention is directed to the serious problem of solanaceous crops continuous cropping obstacle during facility cultivation, can will be of the invention Microbial bacterial agent is applied using seed dressing, root dipping, with base fertilizer application, pouring root or with the top dressing mode of water-fertilizer integral to root Near, and can primary or multiple applications, the generation of prevention continuous cropping root rot is all played in solanaceous crops entire breeding time, is protected It holds the wet of soil and is more advantageous to colonizing and its activity holding, the reduction applications of pesticide, prevention continuous cropping soil-borne disease for functional microorganism Harmful generation proposes a kind of microbial bacterial agent for alleviating solanaceous crops continuous cropping obstacle.

Summary of the invention

In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of alleviation solanaceous crops continuous cropping obstacles Microbial bacterial agent method of preparation and use, can preferably alleviate solanaceous crops continuous cropping obstacle, can with root dipping, pouring root, mix bottom Fertilizer perhaps water-fertilizer integral carry out top dressing can primary or multiple applications, base fertilizer can only be made by making up common microbial bacterial agent Difficult disadvantage is applied and colonized, can be sent out in the root rot that the breeding time of entire solanaceous crops all plays prevention continuous cropping obstacle It is raw, and apply conveniently, at the same the auxiliary material in microbial inoculum be conducive to functional microorganism colonize with active enhancing, with solanaceous crops Better effect is applied in water-fertilizer integral punching, and the generation of root rot, promotes the stable high yield of solanaceous crops preferably in prevention continuous cropping.

To achieve the above object, the present invention is using following technical scheme: a kind of to alleviate the micro- of solanaceous crops continuous cropping obstacle Bacteria agent, it is made of the raw material of following parts by weight: 5-40 parts of Methylotrophic bacillus, bacillus subtilis 5- 40 parts, 1-30 parts of bacillus licheniformis, 1-30 parts of colloid bacillus cereus, 1-25 parts of saccharomycete, 1-25 parts of photosynthetic bacteria, humic acid 2-25 parts, 2-25 parts of potassium fulvate, 1-20 parts of potassium humate, 1-15 parts of amino acid fertilizer, 1-10 parts of potassium hydrogen phosphate, modification biological It is 0.5-8 parts of matter charcoal, 1-10 parts of alginic acid, 0.5-3 parts of chitin, 2-8 parts of peptone, microelement 1-5 parts middle.

Potassium fulvate, humic acid and the potassium humate is biochemical treated water-soluble substances.

The alginic acid is using brown alga as raw material, using full cell physical disruption methods, content release, by shape is concentrated At middle low temperature drying after algae essence concentrate, the active skull cap components of seaweed are kept as far as possible.

The chitin is the shell dilute hydrochloric acid removing calcium carbonate of the crustaceans such as shrimp crab, with hot diluted alkaline deproteination Matter, then be made through decolorization.

The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, sulfuric acid Ferrous iron, manganese sulfate, cerium nitric acid rare earth are according to 10~20 parts: 10~20 parts: 5~10 parts: 5~10 parts: 1~5 part: 0.2 of mass ratio ~3 parts: 0.2~3 parts: 0.5~3 part: 0.5~2 parts are mixed；

The potassium hydrogen phosphate is potassium dihydrogen phosphate or dipotassium hydrogen phosphate.

A kind of preparation method of microbial bacterial agent that alleviating solanaceous crops continuous cropping obstacle, it is comprised the steps of:

Step 1: the preparation of Methylotrophic bacillus: the Methylotrophic gemma bar that will be stored under -4 DEG C of environment Bacteria strain is seeded to 30 DEG C of culture 48h, picking single colonie on beef peptone plate and is seeded to beef extract-peptone Liquid Culture In base, beef-protein medium: beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, After temperature is cultivated for 24 hours in the environment of being 20-40 DEG C, required inoculation liquid is obtained；Inoculation liquid is inoculated in equipped with seed culture medium In 300L seeding tank, seed culture medium: glucose 5.0-10.0g/L, peptone 3.0-8.0g/L, yeast extract 3.0-5.0g/L, Beef extract 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.5-1.0g/L, connecing bacterium amount is 5-10%, after connecing bacterium It ferments, the initial pH value 6.5-7.8 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6- 1.6, when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, is added to the fermentation liquid in seeding tank is equipped at this time The 3000L fermentor of fermentation medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 15.0- 30.0g/L, yeast powder 3.0-5.0g/L, sucrose 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.5-1g/ L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.5-1.0g/L, pH 6.5-7.8, the same seeding tank of control condition cultivate 64- 96 hours, as thallus sporulation 90-95%, terminates fermentation, obtain Methylotrophic fermentation of bacillus liquid, it is dense by film After contracting, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 2: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 8.0-15.0g/L, yeast extract 2.0-5.0g/L, beef extract 3.0-5.0g/L, glucose 3.0- 5.0g/L, sodium chloride 2.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01- 0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.8-7.5 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, this When the fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, fermentation medium: beautiful Rice & peanut milk 20.0-30.0g/L, beancake powder 30.0-50.0g/L, yeast powder 3.0-5.0g/L, sucrose 5.0-8.0g/L, MgSO₄· 7H₂O 0.3-0.5g/L, NaH₂PO₄0.5-1.0g/L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01-0.05g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90- When 95%, terminates fermentation, obtain bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, by its powder After broken, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 3: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 5.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, beef extract 3.0- 5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L,K₂HPO₄0.1-0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, The initial pH value 6.5-7.5 of fermentation, 20-45 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, work as seed Tank ferments 30-50 hours, and thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relay supervention ferment, fermentation medium: corn pulp 15.0-30.0g/L, beancake powder 20.0-30.0g/L, yeast Powder 3.0-5.0g/L, sucrose 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂· 2H₂O.1-0.3g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.5, the same seeding tank of control condition are cultivated 64-96 hours, work as bacterium When body sporulation 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；Pass it through film concentration after, then in it is low Temperature is dry, and after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 4: the preparation of colloid bacillus cereus: extremely by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 18-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 8.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, sucrose 3.0-5.0g/ L, beef extract 2.0-5.0g/L, NaH₂PO₄0.2-0.5g/L, MgSO₄·7H₂O 0.2-0.5g/L, connecing bacterium amount is 5-10%, is connect It ferments after bacterium, the initial pH value 6.8-7.5 of fermentation, 20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio is 1: 0.6-1.6, when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, is at this time added to the fermentation liquid in seeding tank 3000L fermentor equipped with fermentation medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 20.0-30.0g/L yeast powder 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.2-0.5g/L, CaCl₂· 2H₂O.2-0.5g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, work as bacterium When body sporulation 90-95%, terminates fermentation, obtain colloid bacillus cereus fermentation liquid；Pass it through film concentration after, then in it is low Temperature is dry, and after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment being seeded to beef peptone and is put down 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on plate, beef-protein medium: Beef extract 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are trained in the environment of temperature is 20-35 DEG C After supporting for 24 hours, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: Glucose 5.0-10.0g/L, peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄· 7H₂O 0.8-1.0g/L, FeSO₄0.03-0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value of fermentation 6.5-7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small When, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the 3000L fermentor equipped with fermentation medium at this time Supervention ferment is relayed, fermentation medium: mung bean sprouts 50.0-80.0g/L (is cooked into bean sprout juice), potato starch 20.0-30.0g/L, sugarcane Sugared 8.0-12.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂·2H₂O 0.1-0.3g/L, pH 6.5-7.8, the same seeding tank of control condition cultivate 64-96 Hour, as thallus sporulation 90-95%, terminates fermentation, obtain saccharomycetes to make fermentation liquid；After passing it through film concentration, then Middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 6: the preparation of photosynthetic bacteria: the Photosynthetic bacterium strain being stored under -4 DEG C of environment has been seeded to seed training In the 1L glass airtight bottle for supporting base, seed culture medium: CH₃COONa 3.0-5.0g/L, NH₄Cl 0.7-1.5g/L, yeast extract 0.5-1.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, KH₂PO₄0.5-0.1g/L, K₂HPO₄0.3- 0.5g/L, CaCl₂·2H₂O 0.1-0.3g/L, the initial pH value 6.5-7.5 of fermentation, control is in the glass airtight bottle of 1L, temperature The fermentation liquid of glass airtight bottle, is added to equipped with hair by 28-35 DEG C of degree, illumination 2800-3600Lx, culture 60-90 hours at this time The 10L glass capsulation bottle of ferment culture medium relays supervention ferment, fermentation medium and condition of culture with the identical of seed culture medium, culture 90-120 hours, terminates fermentation, obtain Fermentation by Photosynthetic Bacteria liquid；After passing it through film concentration, cell concentration >=1,000,000,000 CFU/g liquid Body microbial inoculum, it is spare；

Step 7: the preparation of amino acid fertilizer: amino acid is the animal sources amino acid of commodity, under various ocean fish processing factories Heel, boiling pulp-water are raw material, through microbial fermentation solution and biodegradable protein, after generating some new active materials, spray Mist drying is process, and obtains amino acid fertilizer, spare；

Step 8: the preparation of modified biomass charcoal: modified biomass charcoal is that husk is formed by anaerobism burn incompletely After carbonized substance, then through ball mill ball milling modified biomass charcoal is obtained using the sieve of 500 mesh at nano-scale particle, it is standby With；

Step 9: the preparation of middle microelement: the middle microelement be amino acid chelated calcium, magnesium sulfate, zinc sulfate, Borax, ammonium molybdate, copper sulphate, ferrous sulfate, manganese sulfate, cerium nitric acid rare earth according to 10~20 parts: 10~20 parts: 5 of mass ratio~ 10 parts: 5~10 parts: 1~5 part: 0.2~3 parts: 0.2~3 part: 0.5~3 part 0.5~2 part are mixed, spare；

Step 10: prepared by microbial premix: by above-mentioned Methylotrophic bacillus, bacillus subtilis, lichens Bacillus, colloid bacillus cereus, saccharomycete solid thalli powder, according to certain parts by weight and spare modified biomass Charcoal, humic acid are first mixed, and are then taken certain parts by weight photosynthetic bacteria to be sprayed in mixed raw material again and are placed 24 hours Left and right is uniformly mixed to obtain microbial premix, spare；

Step 11: prepared by potassium fulvate premix: by potassium fulvate, potassium humate, amino acid fertilizer, alginic acid, first Shell element, peptone and potassium hydrogen phosphate, middle microelement are mixed, due to potassium fulvate, amino acid fertilizer and peptone Etc. the sticky feature of moisture absorption is easy to, most potassium hydrogen phosphate and middle micro material can be embedded, be played with micro- life The buffer action of object is further conducive to the activity for keeping functional microorganism and extension function microorganism shelf-life, spare；

Step 13: microbial premix and potassium fulvate premix preparation to be stirred uniformly mixed, can be obtained A kind of microbial bacterial agent for alleviating solanaceous crops continuous cropping obstacle.

It is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent application method can using seed dressing, root dipping, the bottom of with Fertilizer is applied, pouring root or is applied with the top dressing mode of water-fertilizer integral near root, can primary or multiple applications, Solanaceous crops entire breeding time all plays the generation of prevention continuous cropping root rot, is more advantageous to holding soil under the conditions of water-fertilizer integral It is wet convenient in functional microorganism colonizing and its activity is kept, reduce the applications of pesticide, prevent the generation of continuous cropping soil-borne disease.

After adopting the above technical scheme, the invention has the following beneficial effects: with Methylotrophic bacillus, bacillus subtilis Bacterium, bacillus licheniformis, colloid bacillus cereus, saccharomycete, photosynthetic bacteria are strain, by mutual after the independent Fermented Condensed of strain Match, is mixed with modified biomass charcoal, the shelf-life of protection microbial inoculum and active effect, in the fulvic acid for mixing certain number Potassium, potassium humate, amino acid fertilizer, alginic acid, chitin, peptone, potassium hydrogen phosphate, middle microelement raw material, and it is existing Anti- continuous cropping microbial bacterial agent compare, have the advantage that (1) substance used of the invention is largely water-soluble, it is insoluble Agent particle size is smaller, can satisfy pouring root or water-fertilizer integral rushes the requirement applied；(2) of the invention is connected for solanaceous crops Being easy to happen under work causes sickle-like bacteria, silk core fungi, ralstonia solanacearum etc. to breed, and preferred pin is to alleviation solanaceous crops continuous cropping obstacle The cooperation of multiple-microorganism microbial inoculum, not simple proportion combination, synergistic effect, while the physics and chemistry of synergic adjustment soil Matter forms beneficial rhizosphere environment, enhances the disease resistance of solanaceous crops, nutrition-concerted, and water conservation, fertilizer conservation can prevent Solanaceous crops continuous cropping obstacle is alleviated in the generation of solanaceous crops root-rot disease；(3) pass through the mixed of microbial bacterial agent and other raw materials It closes, allows functional microorganism preferably to adsorb and live in be in carrier with modified biomass charcoal and humic acid etc., while using yellow Rotten acid potassium, amino acid fertilizer, peptone are easy to the sticky feature of moisture absorption, it is first mixed with chemical raw material and plays packet It wraps up in effect and humic acid and modified biomass charcoal etc. and suction-operated is risen to functional microorganism, make functional microorganism and chemical fertilizer More preferable isolation is conducive to the activity for keeping functional microorganism and extension function microorganism shelf-life；(4) potassium fulvate, humic acid, The substances such as humic acid potassium, modified biomass charcoal, amino acid fertilizer are conducive to function as functional microorganism is applied together to soil Energy microorganism preferably colonizes in the soil；(5) humic acid, amino acid fertilizer, fulvic acid contain some active groups and function Energy microbial bacterial agent is generating the bioactive substances such as some indole acids, gibberellic acid, can stimulate crop growth, improve enzyme activity Power, the disease-resistant degeneration-resistant effect of enhancing, to take root, growth promoting, flower and fruit protecting all have certain effect, the stable yields for being conducive to solanaceous crops is high It produces.(6) chitin has prevention subterranean pest-insect, the generation to prevent virus diseases, while alginic acid can activate crop root cell, Root activity is improved, is taken root fast, the more root long Gen Baigen of root are strong, can be heavy metal-passivated, continuous cropping obstacle, fertilizer damage, medicine is effectively relieved The adverse environmental factors such as evil, freeze injury and salt damage promote solanaceous crops fruit to shift to an earlier date maturation.

After adopting the above technical scheme, beneficial effect of the present invention can preferably alleviate solanaceous crops continuous cropping obstacle, make up Most microbial bacterial agent is due to being rushed using the absorption of the non-water soluble substances such as turf, husk and plant ash and being applied difficult lack Point, the present invention can satisfy dress seed under solanaceous crops Continuous Mono-cropping, root dipping, with base fertilizer apply, pouring root or with liquid manure one The top dressing mode of change is applied near root together, can primary or multiple applications, be more advantageous under the conditions of water-fertilizer integral The wet convenient for keeping in colonizing for functional microorganism with its activity of soil is kept, reduces the applications of pesticide, it is possible to reduce Solanaceae is made Root rot disease in object greenhouse facility cultivation occurs, and in the cultivation of continuous cropping capsicum, does not apply microbial bacteria relatively Agent has increased production 8.93%, and opposite control efficiency is 59.45%.In the cultivation of continuous cropping eggplant, microbial bacteria is not applied relatively Agent has increased production 8.71%, and opposite control efficiency is respectively 60.67%.Therefore, the present invention alleviates the micro- of solanaceous crops continuous cropping obstacle Bacteria agent is conducive to the high and stable yields of solanaceous crops, the effect of improving quality.

Specific embodiment

Embodiment 1

Present embodiment the technical solution adopted is that, it is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent, It is made of the raw material of following parts by weight: 5 parts of Methylotrophic bacillus, 5 parts of bacillus subtilis, lichens gemma bar 1 part of bacterium, 1 part of colloid bacillus cereus, 1 part of saccharomycete, 1 part of photosynthetic bacteria, 15 parts of humic acid, 25 parts of potassium fulvate, potassium humate 10 parts, 15 parts of amino acid fertilizer, 10 parts of potassium hydrogen phosphate, 0.5 part of modified biomass charcoal, 2 parts of alginic acid, 0.5 part of chitin, albumen 7 parts of peptone, 1 part of middle microelement.

The registration number of the Methylotrophic bacillus 12, bacillus subtilis is CICC10275, lichens gemma bar Bacterium registration number is ATCC11946, and bacillus laterosporus registration number ATCC10249, colloid bacillus cereus registration number is GIM1.16, Saccharomycete is candida utili bacterium registration number ATCC22023, and photosynthetic bacteria is Rhodopseudomonas palustris registration number ATCC17001。

Potassium fulvate, humic acid and the potassium humate is biochemical treated water-soluble substances.

The alginic acid is using brown alga as raw material, using full cell physical disruption methods, content release, by shape is concentrated At middle low temperature drying after algae essence concentrate, the active skull cap components of seaweed are kept as far as possible.

The chitin is the shell dilute hydrochloric acid removing calcium carbonate of the crustaceans such as shrimp crab, with hot diluted alkaline deproteination Matter, then be made through decolorization.

The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, sulfuric acid Ferrous iron, manganese sulfate, cerium nitric acid rare earth are according to 10 parts: 10 parts: 5 parts: 5 parts: 1 part: 0.2 part: 0.2 part: 0.5 parts: 0.5 part of mass ratio It is mixed；

The potassium hydrogen phosphate is potassium dihydrogen phosphate.

A kind of preparation method of microbial bacterial agent that alleviating solanaceous crops continuous cropping obstacle, it is comprised the steps of:

Step 1: the preparation of Methylotrophic bacillus: the Methylotrophic gemma bar that will be stored under -4 DEG C of environment Bacteria strain is seeded to 30 DEG C of culture 48h, picking single colonie on beef peptone plate and is seeded to beef extract-peptone Liquid Culture In base, beef-protein medium: beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, After temperature is cultivated for 24 hours in the environment of being 20-40 DEG C, required inoculation liquid is obtained；Inoculation liquid is inoculated in equipped with seed culture medium In 300L seeding tank, seed culture medium: glucose 5.0g/L, peptone 5.0g/L, yeast extract -5.0g/L, beef extract 3.0g/L, MgSO₄·7H₂O 0.5g/L, K₂HPO₄1.0g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value of fermentation 6.5-7.8,28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small When, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the 3000L fermentor equipped with fermentation medium at this time Supervention ferment is relayed, fermentation medium: corn pulp 30.0g/L, beancake powder 30.0g/L, yeast powder 5.0g/L, sucrose 3.0g/L, MgSO₄·7H₂O 0.3g/L, NaH₂PO₄0.5g/L, CaCl₂·2H₂O 0.2g/L, K₂HPO₄0.5g/L, pH 6.5-7.8, control The same seeding tank of condition is cultivated 64-96 hours, as thallus sporulation 90-95%, is terminated fermentation, is obtained Methylotrophic bud Spore bacillus fermentation liquid, after membrane concentration, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solids Powder, it is spare；

Step 2: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 15.0g/L, yeast extract 5.0g/L, beef extract 3.0g/L, glucose 5.0g/L, sodium chloride 3.0g/L, MgSO₄·7H₂O 0.3g/L, K₂HPO₄0.3g/L, FeCl₃0.01g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, is fermented Initial pH value 6.8-7.5,28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio be 1:0.6-1.6, when seeding tank send out Ferment 30-50 hours, thalli growth was in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relays supervention ferment, fermentation medium: corn pulp 20.0g/L, beancake powder 50.0g/L, yeast powder 5.0g/L, sugarcane Sugared 5.0g/L, MgSO₄·7H₂O 0.3g/L, NaH₂PO₄0.5g/L, CaCl₂·2H₂O 0.1g/L, K₂HPO₄0.3g/L, FeCl₃0.01g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90-95% When, terminate fermentation, obtains bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, is crushed Afterwards, cell concentration >=10,000,000,000 CFU/g solid powders, it is spare；

Step 3: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 10.0g/L, peptone 10.0g/L, yeast extract 3.0g/L, beef extract 3.0g/L, MgSO₄·7H₂O 0.3g/L,K₂HPO₄0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.5-7.5 of fermentation, temperature 20-45 DEG C, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small, at thalli growth In logarithmic phase, the fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, hair at this time Ferment culture medium: corn pulp 20.0g/L, beancake powder 20.0g/L, yeast powder 3g/L, sucrose 3g/L, MgSO₄·7H₂O 0.3g/L, NaH₂PO₄0.3g/L, CaCl₂·2H₂O.1g/L, K₂HPO₄0.3g/L, pH 6.5-7.5, the same seeding tank of control condition cultivate 64- 96 hours, as thallus sporulation 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；Pass it through film concentration Afterwards, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 4: the preparation of colloid bacillus cereus: extremely by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 18-35 DEG C in temperature After cultivating for 24 hours under environment, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, is planted Sub- culture medium: glucose 10.0g/L, peptone 15.0g/L, yeast extract 3.0g/L, sucrose 3.0g/L, beef extract 5.0g/L, NaH₂PO₄0.5g/L, MgSO₄·7H₂O 0.5g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value of fermentation 6.8-7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small When, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the 3000L fermentor equipped with fermentation medium at this time Relay supervention ferment, fermentation medium: corn pulp 30.0g/L, beancake powder 30.0g/L, yeast powder 5.0g/L, MgSO₄·7H₂O 0.5g/L, NaH₂PO₄0.5g/L, CaCl₂·2H₂0.5g/L, K₂HPO₄0.5g/L, pH 6.5-7.8, the same seed of control condition Tank is cultivated 64-96 hours, as thallus sporulation 90-95%, is terminated fermentation, is obtained colloid bacillus cereus fermentation liquid；By its After membrane concentration, then middle low temperature drying, after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment being seeded to beef peptone and is put down 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on plate, beef-protein medium: Beef extract 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are trained in the environment of temperature is 20-35 DEG C After supporting for 24 hours, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: Glucose 10.0g/L, peptone 10.0g/L, yeast extract 5.0g/L, KCl 1.0g/L, MgSO₄·7H₂O 1.0g/L, FeSO₄0.03g/L, connect bacterium amount be 5-10%, ferment after connecing bacterium, the initial pH value 6.5-7.5 of fermentation, 20-40 DEG C of temperature, Revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithm Fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, fermented and cultured at this time by the phase Base: mung bean sprouts 50.0g/L (is cooked into bean sprout juice), potato starch 30.0g/L, sucrose 8.0g/L, yeast extract 3.0g/L, KCl 0.5g/L, MgSO₄·7H₂O 0.3g/L, NaH₂PO₄0.3g/L, CaCl₂·2H₂O 0.1g/L, pH 6.5-7.8, control condition Same seeding tank is cultivated 64-96 hours, as thallus sporulation 90-95%, is terminated fermentation, is obtained saccharomycetes to make fermentation liquid；By its After membrane concentration, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 6: the preparation of photosynthetic bacteria: the Photosynthetic bacterium strain being stored under -4 DEG C of environment has been seeded to seed training In the 1L glass airtight bottle for supporting base, seed culture medium: CH₃COONa 5.0g/L, NH₄Cl 0.7g/L, yeast extract 0.5g/L, KCl 0.5g/L, MgSO₄·7H₂O 0.3g/L, KH₂PO₄0.5g/L, K₂HPO₄0.3g/L, CaCl₂·2H₂O 0.1g/L, at the beginning of fermentation Beginning pH value 6.5-7.5, control in the glass airtight bottle of 1L, 28-35 DEG C of temperature, illumination 2800-3600Lx, culture 60-90 it is small When, the fermentation liquid of glass airtight bottle is added to the relaying supervention ferment of the 10L glass capsulation bottle equipped with fermentation medium, fermentation at this time Culture medium and condition of culture are cultivated 90-120 hours with the identical of seed culture medium, terminate fermentation, obtain Fermentation by Photosynthetic Bacteria Liquid；After passing it through film concentration, cell concentration >=1,000,000,000 CFU/g liquid bacterial agents is spare；

Step 7: the preparation of amino acid fertilizer: amino acid is the animal sources amino acid of commodity, under various ocean fish processing factories Heel, boiling pulp-water are raw material, through microbial fermentation solution and biodegradable protein, after generating some new active materials, spray Mist drying is process, and obtains amino acid fertilizer, spare；

Step 8: the preparation of modified biomass charcoal: modified biomass charcoal is that husk is formed by anaerobism burn incompletely After carbonized substance, then through ball mill ball milling modified biomass charcoal is obtained using the sieve of 500 mesh at nano-scale particle, it is standby With；

Step 9: the preparation of middle microelement: the middle microelement be amino acid chelated calcium, magnesium sulfate, zinc sulfate, Borax, ammonium molybdate, copper sulphate, ferrous sulfate, manganese sulfate, cerium nitric acid rare earth are according to 10 parts: 10 parts: 5 parts: 5 parts: 1 part of mass ratio: 0.2 part: 0.2 part: 0.5 part: 0.5 part is mixed, spare；

Step 10: prepared by microbial premix: by above-mentioned Methylotrophic bacillus, bacillus subtilis, lichens Bacillus, colloid bacillus cereus, saccharomycete thalli powder are according to certain parts by weight and spare modified biomass charcoal, corruption Phytic acid is first mixed, then take again certain parts by weight photosynthetic bacteria be sprayed in mixed raw material place 24 hours or so it is mixed Uniform microbial premix is closed, it is spare；

Step 11: prepared by potassium fulvate premix: by potassium fulvate, potassium humate, amino acid fertilizer, alginic acid, first Shell element, peptone and potassium hydrogen phosphate, middle microelement are mixed, due to potassium fulvate, amino acid fertilizer and peptone Etc. the sticky feature of moisture absorption is easy to, most potassium hydrogen phosphate and middle micro material can be embedded, be played with micro- life The buffer action of object is further conducive to the activity for keeping functional microorganism and extension function microorganism shelf-life, spare；

Step 13: microbial premix and potassium fulvate premix preparation to be stirred uniformly mixed, can be obtained A kind of microbial bacterial agent for alleviating solanaceous crops continuous cropping obstacle.

The microbial bacterial agent obtained for alleviating solanaceous crops continuous cropping obstacle according to the method described above, meets following index:

(1) organic matter >=45%；

(2)N+P₂O₅+K₂O >=12%；

(3) moisture content≤25%；

(4) fineness >=85%；

(5) miscellaneous bacteria number≤15%；

(6) amino acid >=1.5%

(7) effectively bacterium number >=1,000,000,000/g；

(8) validity period is >=12 months.

Points for attention: the high organic complex microorganism punching property the applied fertilizer of the present embodiment does chasing after for solanaceous crops facility cultivation Fertilizer punching application, suitably keeps ground moistening after application, the high organic complex microorganism punching property applied of the present embodiment can be improved The function and fertilizer efficiency of fertilizer.Due to containing microbial flora in special fertilizer, cannot mix make with fungicide or antibacterial material With.Pay attention to avoiding strong illumination when preservation, prevent with strong acid and strong base stack etc..

Embodiment 2:

Present embodiment the technical solution adopted is that, it is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent, It is made of the raw material of following parts by weight: 20 parts of Methylotrophic bacillus, 10 parts of bacillus subtilis, lichens gemma 5 parts of bacillus, 5 parts of colloid bacillus cereus, 5 parts of saccharomycete, 5 parts of photosynthetic bacteria, 15 parts of humic acid, 10 parts of potassium fulvate, humic acid 5 parts of potassium, 5 parts of amino acid fertilizer, 1 part of potassium hydrogen phosphate, 3 parts of modified biomass charcoal, 5 parts of alginic acid, 2 parts of chitin, peptone 2 Part, 2 parts of middle microelement.

The registration number of the Methylotrophic bacillus 12, bacillus subtilis is CICC10275, lichens gemma bar Bacterium registration number is ATCC11946, and bacillus laterosporus registration number ATCC10249, colloid bacillus cereus registration number is GIM1.16, Saccharomycete is candida utili bacterium registration number ATCC22023, and photosynthetic bacteria is Rhodopseudomonas palustris registration number ATCC17001。

Potassium fulvate, humic acid and the potassium humate is biochemical treated water-soluble substances.

The alginic acid is using brown alga as raw material, using full cell physical disruption methods, content release, by shape is concentrated At middle low temperature drying after algae essence concentrate, the active skull cap components of seaweed are kept as far as possible.

The chitin is the shell dilute hydrochloric acid removing calcium carbonate of the crustaceans such as shrimp crab, with hot diluted alkaline deproteination Matter, then be made through decolorization.

The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, sulfuric acid Ferrous iron, manganese sulfate, cerium nitric acid rare earth are according to 20 parts: 20 parts: 10 parts: 10 parts: 5 parts: 3 parts: 3 parts: 3 parts of mass ratio: 2 parts are mixed It closes；

The potassium hydrogen phosphate is dipotassium hydrogen phosphate.

The microbial bacterial agent obtained for alleviating solanaceous crops continuous cropping obstacle according to the method described above, meets following index:

(1) organic matter >=50%；

(2)N+P₂O₅+K₂O >=8%；

(3) moisture content≤25%；

(4) fineness >=85%；

(5) miscellaneous bacteria number≤15%；

(6) amino acid >=0.5%

(7) effectively bacterium number >=2,000,000,000/g；

(8) validity period is >=12 months.

The present embodiment is not directed to place, with embodiment 1.

Embodiment 3

The experimental program and application effect that the microbial bacterial agent of the present embodiment production is applied with capsicum water-fertilizer integral:

(1) experimental design

The planting base greenhouse cultivation (first year cultivation is capsicum) tested in salon, Liuyang City carries out, and experimental cultivar is capsicum Fine work nine, spacing in the rows 45cm, line-spacing 50cm.Test sets 3 processing: control 1: not applying microbial bacterial agent；Control 2: commodity are resisted For the microbial bacterial agent of continuous cropping with base fertilizer applied once, living bacteria count is 1,000,000,000/g, and dosage is 4kg/ mus；Processing 1: this hair is applied In bright microbial bacterial agent described in embodiment 1 with water-fertilizer integral top dressing when apply, living bacteria count is 1,000,000,000/g, and dosage is It 4kg/ mus, is applied twice after transplanting seedling-slowing stage and after the maximum fruit harvesting phase respectively, water management, dose and fertilizer application frequency It is all identical.Every processing is repeated 3 times, and amounts to 9 cells, and every plot area is 20m².Capsicum after record harvests every time in detail produces Amount takes first fruiting period to measure the plant height (length of basal part of stem to growing point) of each cell capsicum, stem thickness (rugosity of basal part of stem) and newly Foliation number plays the 5th SPAD value.It produces fruit later period collection rhizosphere surrounding soil and measures bacterium, fungi, actinomyces, each cell 3 Point sampling, every investigation are connected 7 plants, are classified and are investigated according to wilt disease occurring degree difference as unit of strain, and record investigates total strain Diseased plant numbers several, at different levels.Stage division:

0 grade: complete stool is disease-free；

1 grade: the slight browning of basal part of stem, chili growth are normal；

3 grades: the obvious browning of basal part of stem, it is with softening and slightly rotten；

5 grades: the obvious browning of basal part of stem and rot, lobus cardiacus wither hang down crispatura；

7 grades: basal part of stem browning rots, and the withered or yellowish-brown of complete stool blueness is withered.

Diseased plant rate (%)=diseased plant number/investigation total strain number × 100%；

Disease index (%)=∑ (diseased plant numbers at different levels × opposite value of series)/(investigation total strain number × 7) × 100%；

Opposite control efficiency (%)=(control disease incidence-processing disease incidence)/control disease incidence × 100%.

Other not specified index determinings are carried out with reference to the methods of related national standard.

(2) main result

Influence of 1 microbial bacterial agent of table to continuous cropping chili growth and yield index of correlation

Note: institute's column data is 3 duplicate average values in table, and different letters indicate that difference reaches significant water after same column numerical value It is flat, similarly hereinafter.

As can be seen from Table 1, the continuous cropping capsicum tree that embodiment 1 is handled is high, set stem thickness and the fresh yield of per hectare is all significantly high In not applying microbial inoculum processing, the fresh yield of per hectare has increased production 8.93% than not applying microbial bacterial agent processing, life more micro- than general goods The processing of object microbial inoculum has increased production 5.29%.Illustrate embodiment 1 under dose and all identical situation of fertilizer application frequency, effect of increasing production is bright It is aobvious.

Influence of 2 microbial bacterial agent of table to continuous cropping capsicum rhizosphere microorganism

As can be seen from Table 2, the continuous cropping capsicum soil bacteria, actinomyces of the processing of embodiment 1 all do not apply microbial bacteria significantly Agent and the processing of general goods microbial bacterial agent.Mainly embodiment 1 handles microbial bacterial agent in suitable ingredients and cooperates item Part is more advantageous to its colonizing in continuous cropping soil, to dramatically increase the quantity of soil bacteria and actinomyces, enhancing soil is micro- Bioactivity and enzymatic activity improve soil texture, are conducive to the conversion of substance in soil, improve the fertility of soil.

Influence of 3 microbial bacterial agent of table to continuous cropping capsicum wilt disease index

As can be seen from Table 3, the microbial bacterial agent prepared with embodiment 1 is applied with water-fertilizer integral into capsicum soil, The disease incidence and disease index of its continuous cropping capsicum do not apply microbial bacterial agent substantially lower than and apply general goods microbial bacterial agent, Not applying microbial bacterial agent relatively and applying the opposite control efficiency of general goods microbial bacterial agent is respectively 59.45% and 45.97%. Therefore, it applies after microbial bacterial agent punching of the invention applies, the growth of capsicum can be promoted, reduce the hair of continuous cropping capsicum wilt It is raw.

Embodiment 4

The experimental program and application effect that the microbial bacterial agent of the present embodiment production is applied with eggplant water-fertilizer integral:

(1) experimental design

It tests the planting base greenhouse cultivation in village, salon, Liuyang City and carries out (cultivation in head 2 years is all eggplant), experimental cultivar It is eggplant spring eggplant ten No. six, spacing in the rows 50cm, line-spacing 60cm.Test sets 3 processing: control 1: not applying microbial bacterial agent；Control 2: By the microbial bacterial agent of the anti-continuous cropping of commodity with base fertilizer applied once, living bacteria count is 1,000,000,000/g, and dosage is 4kg/ mus；Processing 1: It is applied when applying in the present invention microbial bacterial agent as described in example 2 with water-fertilizer integral top dressing, living bacteria count is 2,000,000,000/g, Dosage is 4kg/ mu, applies after transplant seedling-slowing stage and applies respectively after the maximum fruit harvesting phase, water management, dose and applies Fertile number is all identical.Every processing is repeated 3 times, and amounts to 9 cells, and every plot area is 20m².After record harvests every time in detail Eggplant yield takes first fruiting period to measure plant height (length of basal part of stem to growing point) stem thickness (rugosity of basal part of stem) of each cell eggplant And new foliation number plays the 5th SPAD value.It produces fruit later period collection rhizosphere surrounding soil and measures bacterium, fungi, actinomyces, each 3 points of cell samplings, every investigation are connected 7 plants, are classified and are investigated according to wilt disease occurring degree difference as unit of strain, record investigation Total strain number, diseased plant number at different levels.Stage division:

0 grade: complete stool is disease-free；

1 grade: the slight browning of basal part of stem, eggplant growth are normal；

3 grades: the obvious browning of basal part of stem, it is with softening and slightly rotten；

5 grades: the obvious browning of basal part of stem and rot, lobus cardiacus wither hang down crispatura；

7 grades: basal part of stem browning rots, and the withered or yellowish-brown of complete stool blueness is withered.

Diseased plant rate (%)=diseased plant number/investigation total strain number × 100%；

Disease index (%)=∑ (diseased plant numbers at different levels × opposite value of series)/(investigation total strain number × 7) × 100%；

Opposite control efficiency (%)=(control disease incidence-processing disease incidence)/control disease incidence × 100%.

Other not specified index determinings are carried out with reference to the methods of related national standard.

(2) main result

1 microbial bacterial agent of table is grown to eggplant and the influence of yield index of correlation

Note: institute's column data is 3 duplicate average values in table, and different letters indicate that difference reaches significant water after same column numerical value It is flat, similarly hereinafter.

As can be seen from Table 1, the continuous cropping eggplant tree that embodiment 2 is handled is high, set stem thickness and the fresh yield of per hectare is all significantly high In not applying microbial inoculum processing, the fresh yield of per hectare has increased production 8.71% than not applying microbial bacterial agent processing, life more micro- than general goods The processing of object microbial inoculum has increased production 4.45%.Illustrate embodiment 1 under dose and all identical situation of fertilizer application frequency, eggplant volume increase is imitated Fruit is obvious.

Influence of 2 microbial bacterial agent of table to eggplant rhizosphere microorganism

As can be seen from Table 2, the continuous cropping eggplant soil bacteria, fungi of the processing of embodiment 2 all do not apply microbial bacterial agent significantly With the processing of general goods microbial bacterial agent.Mainly embodiment 1 handles microbial bacterial agent in suitable ingredients and cooperates condition It is more advantageous to its colonizing in continuous cropping soil, to dramatically increase the quantity of soil bacteria and fungi, enhances edaphon Activity and enzymatic activity improve soil texture, are conducive to the conversion of substance in soil, improve the fertility of soil.

Influence of 3 microbial bacterial agent of table to wilt of eggplant disease index

As can be seen from Table 3, the microbial bacterial agent prepared with embodiment 2 is applied with water-fertilizer integral into eggplant soil, The disease incidence and disease index of its continuous cropping eggplant do not apply microbial bacterial agent substantially lower than and apply general goods microbial bacterial agent, Not applying microbial bacterial agent relatively and applying the opposite control efficiency of general goods microbial bacterial agent is respectively 60.67% and 43.57%. Therefore, it applies after microbial bacterial agent punching of the invention applies, the growth of eggplant can be promoted, reduce the hair of continuous cropping wilt of eggplant It is raw.

The above is only used to illustrate the technical scheme of the present invention and not to limit it, and those of ordinary skill in the art are to this hair The other modifications or equivalent replacement that bright technical solution is made, as long as it does not depart from the spirit and scope of the technical scheme of the present invention, It is intended to be within the scope of the claims of the invention.

Claims (7)

1. it is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent, which is characterized in that it by following parts by weight former material Material composition: 5-40 parts of Methylotrophic bacillus, 5-40 parts of bacillus subtilis, 1-30 parts of bacillus licheniformis, colloid bud 1-30 parts of spore bacillus, 1-25 parts of saccharomycete, 1-25 parts of photosynthetic bacteria, 2-25 parts of humic acid, 2-25 parts of potassium fulvate, potassium humate 1-20 parts, 1-15 parts of amino acid fertilizer, 1-10 parts of potassium hydrogen phosphate, 0.5-8 parts of modified biomass charcoal, 1-10 parts of alginic acid, crust It is 0.5-3 parts plain, 2-8 parts of peptone, microelement 1-5 parts middle；Potassium fulvate, humic acid and the potassium humate is at biochemistry Water-soluble substances after reason；The alginic acid is using brown alga as raw material, and using full cell physical disruption methods, content discharges, The middle low temperature drying after concentration forms algae essence concentrate keeps the active skull cap components of seaweed as far as possible；The crust Element is the shell dilute hydrochloric acid removing calcium carbonate of the crustaceans such as shrimp crab, is made with hot diluted alkaline deproteination matter, then through decolorization； The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, ferrous sulfate, sulfuric acid Manganese, cerium nitric acid rare earth are according to 10~20 parts: 10~20 parts: 5~10 parts: 5~10 parts: 1~5 part: 0.2~3 part: 0.2 of mass ratio ~3 parts: 0.5~3 parts: 0.5~2 part are mixed；The potassium hydrogen phosphate is potassium dihydrogen phosphate or dipotassium hydrogen phosphate.

2. it is according to claim 1 it is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent, which is characterized in that it by The raw material of following parts by weight form: 5 parts of Methylotrophic bacillus, 5 parts of bacillus subtilis, bacillus licheniformis 1 Part, 1 part of colloid bacillus cereus, 1 part of saccharomycete, 1 part of photosynthetic bacteria, 15 parts of humic acid, 25 parts of potassium fulvate, potassium humate 10 Part, 15 parts of amino acid fertilizer, 10 parts of potassium hydrogen phosphate, 0.5 part of modified biomass charcoal, 2 parts of alginic acid, 0.5 part of chitin, peptone 7 parts, 1 part of middle microelement；The registration number of the Methylotrophic bacillus 12, bacillus subtilis is CICC10275, Bacillus licheniformis registration number is ATCC11946, bacillus laterosporus registration number ATCC10249, colloid bacillus cereus registration number For GIM1.16, saccharomycete is candida utili bacterium registration number ATCC22023, and photosynthetic bacteria is Rhodopseudomonas palustris registration Number ATCC17001；Potassium fulvate, humic acid and the potassium humate is biochemical treated water-soluble substances；The sea Alginic acid is using brown alga as raw material, using full cell physical disruption methods, content release, after concentration forms algae essence concentrate Middle low temperature drying keeps the active skull cap components of seaweed as far as possible；The chitin is that the shell of the crustaceans such as shrimp crab is dilute Hydrochloric acid removes calcium carbonate, is made with hot diluted alkaline deproteination matter, then through decolorization；The middle microelement is amino acid chela Calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, ferrous sulfate, manganese sulfate, cerium nitric acid rare earth are closed according to mass ratio 10 Part: 10 parts: 5 parts: 5 parts: 1 parts: 0.2 part: 0.2 part: 0.5 part: 0.5 parts are mixed；The potassium hydrogen phosphate is potassium dihydrogen phosphate.

3. it is according to claim 1 it is a kind of alleviate solanaceous crops continuous cropping obstacle microbial bacterial agent, which is characterized in that it by The raw material of following parts by weight form: being made of the raw material of following parts by weight: 20 parts of Methylotrophic bacillus, withered 10 parts of careless bacillus, 5 parts of bacillus licheniformis, 5 parts of colloid bacillus cereus, 5 parts of saccharomycete, 5 parts of photosynthetic bacteria, humic acid 15 Part, 10 parts of potassium fulvate, 5 parts of potassium humate, 5 parts of amino acid fertilizer, 1 part of potassium hydrogen phosphate, 3 parts of modified biomass charcoal, alginic acid 5 Part, 2 parts of chitin, 2 parts of peptone, 2 parts of middle microelement；The Methylotrophic bacillus 12, bacillus subtilis Registration number be CICC10275, bacillus licheniformis registration number be ATCC11946, bacillus laterosporus registration number ATCC10249, colloid bacillus cereus registration number are GIM1.16, and saccharomycete is candida utili bacterium registration number ATCC22023, Photosynthetic bacteria is Rhodopseudomonas palustris registration number ATCC17001；Potassium fulvate, humic acid and the potassium humate is biochemistry Treated water-soluble substances；The alginic acid is using brown alga as raw material, and using full cell physical disruption methods, content is released It puts, the middle low temperature drying after concentration forms algae essence concentrate, keeps the active skull cap components of seaweed as far as possible；The first Shell element is the shell dilute hydrochloric acid removing calcium carbonate of the crustaceans such as shrimp crab, with hot diluted alkaline deproteination matter, then through decolorization system ?；The middle microelement be amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonium molybdate, copper sulphate, ferrous sulfate, Manganese sulfate, cerium nitric acid rare earth are according to 20 parts: 20 parts: 10 parts: 10 parts: 5 parts: 3 parts: 3 parts: 3 parts of mass ratio: 2 parts are mixed；Institute Stating potassium hydrogen phosphate is dipotassium hydrogen phosphate.

4. a kind of preparation method for the microbial bacterial agent for alleviating solanaceous crops continuous cropping obstacle, which is characterized in that it includes following step It is rapid:

Step 1: the preparation of Methylotrophic bacillus: the Methylotrophic bacillus bacterium that will be stored under -4 DEG C of environment Strain is seeded to 30 DEG C of culture 48h, picking single colonie on beef peptone plate and is seeded in beef extract-peptone fluid nutrient medium, Beef-protein medium: beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, in temperature After cultivating for 24 hours in the environment of being 20-40 DEG C, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L equipped with seed culture medium In seeding tank, seed culture medium: glucose 5.0-10.0g/L, peptone 3.0-8.0g/L, yeast extract 3.0-5.0g/L, beef Cream 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.5-1.0g/L, connecing bacterium amount is 5-10%, is carried out after connecing bacterium Fermentation, the initial pH value 6.5-7.8 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, When seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation at this time The 3000L fermentor of culture medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 15.0-30.0g/ L, yeast powder 3.0-5.0g/L, sucrose 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.5-1g/L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.5-1.0g/L, pH 6.5-7.8, the same seeding tank of control condition cultivate 64-96 Hour, as thallus sporulation 90-95%, terminates fermentation, obtain Methylotrophic fermentation of bacillus liquid, be concentrated by film Afterwards, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 2: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 20-35 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: peptone 8.0-15.0g/L, yeast extract 2.0-5.0g/L, beef extract 3.0-5.0g/L, glucose 3.0-5.0g/L, Sodium chloride 2.0-5.0 g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃ 0.01-0.05g/L, Connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.8-7.5 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/ Min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, at this time by seeding tank In fermentation liquid be added to the 3000L fermentor equipped with fermentation medium relaying supervention ferment, fermentation medium: corn pulp 20.0- 30.0g/L, beancake powder 30.0-50.0g/L, yeast powder 3.0-5.0g/L, sucrose 5.0-8.0g/L, MgSO₄·7H₂O 0.3- 0.5g/L, NaH₂PO₄0.5-1.0g/L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃ 0.01- 0.05g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90-95%, knot Beam fermentation, obtains bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, after being crushed, thallus Concentration >=10,000,000,000 CFU/g solid powders, it is spare；

Step 3: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 20-30 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: glucose 5.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, beef extract 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L,K₂HPO₄0.1-0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, at the beginning of fermentation Beginning pH value 6.5-7.5,20-45 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank ferments 30-50 hours, thalli growth was in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relays supervention ferment, fermentation medium: corn pulp 15.0-30.0g/L, beancake powder 20.0-30.0g/L, yeast powder 3.0-5.0g/L sucrose 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂· 2H₂O.1-0.3g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.5, the same seeding tank of control condition are cultivated 64-96 hours, work as bacterium When body sporulation 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；Pass it through film concentration after, then in it is low Temperature is dry, and after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 4: the preparation of colloid bacillus cereus: by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 18-35 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: glucose 8.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, sucrose 3.0-5.0g/L, ox Meat extract 2.0-5.0g/L, NaH₂PO₄0.2-0.5g/L, MgSO₄·7H₂O 0.2-0.5g/L, connecing bacterium amount is 5-10%, after connecing bacterium It ferments, the initial pH value 6.8-7.5 of fermentation, 20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6- 1.6, when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, is added to the fermentation liquid in seeding tank is equipped at this time The 3000L fermentor of fermentation medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 20.0- 30.0g/L, yeast powder 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.2-0.5g/L, CaCl₂·2H₂ O.2-0.5g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, work as thallus When sporulation 90-95%, terminates fermentation, obtain colloid bacillus cereus fermentation liquid；Pass it through film concentration after, then in low temperature Dry, after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment is seeded on beef peptone plate 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium, beef-protein medium: beef Cream 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are cultivated for 24 hours in the environment of temperature is 20-35 DEG C Afterwards, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: grape Sugared 5.0-10.0g/L, peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.8-1.0g/L, FeSO₄0.03-0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.5- of fermentation 7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small, bacterium Body growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the relaying of the 3000L fermentor equipped with fermentation medium at this time Supervention ferment, fermentation medium: mung bean sprouts 50.0-80.0g/L(is cooked into bean sprout juice), potato starch 20.0-30.0g/L, sucrose 8.0-12.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄ 0.3-0.5g/L, CaCl₂·2H₂O 0.1-0.3g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, when When thallus sporulation 90-95%, terminates fermentation, obtain saccharomycetes to make fermentation liquid；After passing it through film concentration, then middle low temperature is dry Dry, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 6: the preparation of photosynthetic bacteria: the Photosynthetic bacterium strain being stored under -4 DEG C of environment has been seeded to seed culture medium 1L glass airtight bottle in, seed culture medium: CH₃COONa 3.0-5.0g/L, NH₄Cl 0.7-1.5g/L, yeast extract 0.5- 1.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, KH₂PO₄0.5-0.1g/L, K₂HPO₄ 0.3-0.5g/ L, CaCl₂·2H₂O 0.1-0.3g/L, the initial pH value 6.5-7.5 of fermentation are controlled in the glass airtight bottle of 1L, temperature 28- The fermentation liquid of glass airtight bottle, is added to equipped with fermented and cultured by 35 DEG C, illumination 2800-3600Lx, culture 60-90 hours at this time The 10L glass capsulation bottle of base relays supervention ferment, fermentation medium and condition of culture with the identical of seed culture medium, cultivates 90-120 Hour, terminate fermentation, obtains Fermentation by Photosynthetic Bacteria liquid；After passing it through film concentration, cell concentration >=1,000,000,000 CFU/g liquid bacterias Agent, it is spare；

Step 7: the preparation of amino acid fertilizer: amino acid is the animal sources amino acid of commodity, with various ocean fish processing factories leftover bits and pieces Material, boiling pulp-water are raw material, spraying after generating some new active materials through microbial fermentation solution and biodegradable protein Drying is process, and obtains amino acid fertilizer, spare；

Step 8: the preparation of modified biomass charcoal: modified biomass charcoal is the charcoal that husk passes through that anaerobism burn incompletely is formed After compound matter, then through ball mill ball milling modified biomass charcoal is obtained using the sieve of 500 mesh at nano-scale particle, it is spare；

Step 9: the preparation of middle microelement: the middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, boron Sand, ammonium molybdate, copper sulphate, ferrous sulfate, manganese sulfate, cerium nitric acid rare earth are according to 10~20 parts: 10~20 parts: 5~10 of mass ratio Part: 5~10 parts: 1~5 parts: 0.2~3 part: 0.2~3 parts: 0.5~3 part 0.5~2 part are mixed, spare；

Step 10: prepared by microbial premix: by above-mentioned Methylotrophic bacillus, bacillus subtilis, lichens gemma Bacillus, colloid bacillus cereus, saccharomycete solid thalli powder, according to certain parts by weight and spare modified biomass charcoal, Humic acid is first mixed, and is then taken certain parts by weight photosynthetic bacteria to be sprayed in mixed raw material again and is placed 24 hours or so It is uniformly mixed to obtain microbial premix, it is spare；

Step 11: potassium fulvate premix prepare: by potassium fulvate, potassium humate, amino acid fertilizer, alginic acid, chitin, Peptone is mixed with potassium hydrogen phosphate, middle microelement, since potassium fulvate, amino acid fertilizer and peptone etc. hold very much Sticky feature easy to moisture absorption can embed most potassium hydrogen phosphate and middle micro material, play with microorganism every From effect, be further conducive to the activity for keeping functional microorganism and extension function microorganism shelf-life, it is spare；

Step 13: microbial premix and potassium fulvate premix preparation to be stirred uniformly mixed, one kind can be obtained Alleviate the microbial bacterial agent of solanaceous crops continuous cropping obstacle.

5. a kind of application method for the microbial bacterial agent for alleviating solanaceous crops continuous cropping obstacle, it is characterised in that: using seed dressing, dip in Root is applied with base fertilizer application, pouring root or with the top dressing mode of water-fertilizer integral near root, can be primary or multiple Application is all played the generation of prevention continuous cropping root rot in solanaceous crops entire breeding time, is more advantageous under the conditions of water-fertilizer integral The wet convenient for keeping in colonizing for functional microorganism with its activity of soil is kept, the applications of pesticide is reduced, prevents continuous cropping soil-borne disease Harmful generation.

6. a kind of microbial bacterial agent of alleviation solanaceous crops continuous cropping obstacle described in -5 according to claim 1, it is characterised in that: on State experimental program that the microbial bacterial agent of alleviation solanaceous crops continuous cropping obstacle of production a kind of is applied with capsicum water-fertilizer integral and Application effect.

7. a kind of microbial bacterial agent of alleviation solanaceous crops continuous cropping obstacle described in -5 according to claim 1, it is characterised in that: on State experimental program that the microbial bacterial agent of alleviation solanaceous crops continuous cropping obstacle of production a kind of is applied with eggplant water-fertilizer integral and Application effect.