CN111034441A - Irrigation method for continuously repairing microbial flora in continuous cropping soil of melon crops - Google Patents
Irrigation method for continuously repairing microbial flora in continuous cropping soil of melon crops Download PDFInfo
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Abstract
The invention relates to an irrigation method for repairing microbial floras in continuous cropping soil of melon crops, which comprises the following steps: (1) preparing a burkholderia biological agent; (2) mixing the loaded particles; (3) preparing irrigation water fertilizer, wherein the bacterial concentration of Burkholderia in the irrigation water fertilizer is 1 multiplied by 10‑1×103cfu/ml, seaweed fertilizer 0.0001-0.01 g/L; (4) and applying irrigation water and fertilizer, wherein the irrigation water and fertilizer is subjected to a hose drip irrigation method, and the irrigation amount of the water and fertilizer at one time is 7-15 cubic meters per mu. The method adopts the irrigation water containing the probiotics with the repairing effect to irrigate crops, continuously repairs the continuous cropping soil, reduces the growth speed of phenolic acid substances in the soil of the crops, increases the abundance and diversity of microbial populations in the soil, has good repairing effect, promotes the growth and disease resistance of the crops, and increases the melon plantingNumber of female flowers of the strain.
Description
Technical Field
The invention relates to the field of soil microbial flora remediation, in particular to an irrigation method for continuously remedying the microbial flora in the soil for continuous cropping of melon crops.
Background
Continuous cropping obstacles have been an important cause for limiting the yield of greenhouse crops, and the cause of continuous cropping obstacles is very complex and is the result of the combined action of multiple factors. Research has shown that autotoxicity and soil microflora imbalance are important factors for continuous cropping obstacle. The activity and diversity of microorganisms are important indexes for measuring the health condition of soil, soil microorganisms are sensitive to the change of the external environment, and under the continuous cropping condition, root secretion and stubble decomposition products are continuously accumulated in the soil, so that the activity and diversity of the microorganisms are changed, beneficial bacteria are reduced, harmful bacteria are increased, and continuous cropping obstacles are generated.
Biological soil remediation agents and treatment are one of important remediation methods for continuous cropping obstacles, the former methods all adopt effective times of application to influence soil microbial populations, the colonization and propagation of soil beneficial bacteria require the accumulation of the number of floras, and in the process of plant growth, if the propagation speed of the soil remediation beneficial bacteria is lower than the consumption speed, the application effect of the soil remediation agent can be greatly reduced, so that the probiotic bacteria need to be continuously supplemented and the soil environment of the probiotic bacteria needs to be optimized, and a technical method for continuously remedying the continuous cropping soil microbial populations through an irrigation method is lacked at present.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention aims to provide an irrigation method for continuously repairing the microbial flora in the continuous cropping soil of the melon crops.
The invention aims to realize the irrigation method for repairing the microbial flora in the continuous cropping soil of the melon crops by the following technical scheme, which has the technical key points that: the method comprises the following steps:
(1) preparation of biological agent
The biological agent is fermentation liquor of Burkholderia, wherein Burkholderia (Burkholderia phytof ormans) has a preservation unit: the general microbiological center of China Committee for culture Collection of microorganisms, the address is No. 3 of West Lu No.1 of North Chen of the Chaozhou region, Beijing, the institute of microbiology of China academy of sciences, the preservation number is CGMCC No.15326, the strain number is 20180101 Liao CK1, and the preservation date is 29 months and 1 month in 2018;
the formula of the biological agent culture medium comprises: 5g/L of tryptone, 5g/L of yeast extract, 10-30g/L of seaweed fertilizer powder and 2-5g/L of corn starch, and adjusting the pH value to be neutral; sterilizing culture medium, inoculating Burkholderia, fermenting at 28 deg.C under stirring speed of 120rpm for 5 days to obtain bacterial suspension, and adjusting the concentration of the bacterial suspension to 6.0-8.0 × 108cfu/ml, wherein the grain diameters of the seaweed fertilizer powder and the corn starch are 150-200 meshes;
(2) mixed load particles
The method comprises the steps of loading particles with the particle size of 2.5-6mm, loading any one of ceramic carrier particles, silica gel carrier particles and activated carbon carrier particles into a bacterium bag, sealing and sterilizing the loaded particles, placing the bacterium bag into a biological bacterium agent, horizontally shaking and culturing, immersing the loaded particles in the biological bacterium agent, carrying out reciprocating shaking for 50-70 times/minute, carrying out 8-12cm stroke, carrying out 2-5 days at 4-10 ℃, draining redundant bacterium liquid to obtain a loaded particle bacterium bag loaded with bacterial manure, and storing for later use at 4 ℃.
The aperture of the biological fungus bag is 1-2mm, the diameter of the fungus bag is 7-9cm, and carrier particles loaded with probiotics are filled in the fungus bag;
(3) preparation of irrigation water fertilizer
The irrigation water and fertilizer is obtained by adopting a water and fertilizer preparation device, and the water and fertilizer preparation device comprises a water and fertilizer collecting box and a water and fertilizer diluting box; the water and fertilizer collecting box comprises a cuboid water tank body, a horizontal supporting plate is arranged on the inner wall of the lower portion of the front side of the water tank, two symmetrical grooved tracks are arranged on two sides of the water tank, the bottom ends of the grooved tracks are arranged above the supporting plate, and the top ends of the grooved tracks are arranged at the top ends of the rear side of the water tank; a water and fertilizer penetration inserting plate is inserted between the two strip groove tracks, the water and fertilizer penetration inserting plate is provided with sieve cylinders which are arranged at intervals, the bottom of each sieve cylinder is closed, the top of each sieve cylinder is provided with an opening, sieve holes are formed in the surface of each sieve cylinder, the aperture of each sieve hole is 1-1.5cm, and the diameter of each sieve cylinder is 7-10 cm; the bottom of the water tank is provided with a water outlet; the water-fertilizer diluting box is provided with a water inlet, a water filling port and a water outlet, the water inlet of the water-fertilizer diluting box is connected with the water outlet of the water-fertilizer collecting box and the water inlet of the water-fertilizer diluting box, and the water inlet of the water-fertilizer diluting box is lower than or flush with the water outlet of the water-fertilizer collecting box;
biological bacterium bags are inserted into the sieve cylinders of the water and fertilizer penetration inserting plates, the water and fertilizer penetration inserting plates are inserted into the tracks with grooves on two sides of the water tank, water is injected above the water tank, and water flows into the lower part of the water and fertilizer collecting box through the water and fertilizer penetration inserting plates to obtain water and fertilizer; the water fertilizer flows into a water fertilizer dilution box, clear water is injected through a water injection port, and irrigation water fertilizer is obtained, wherein the bacterial concentration of Burkholderia in the irrigation water fertilizer is 1 multiplied by 10-1-103cfu/ml, seaweed fertilizer 0.0001-0.01 g/L;
(4) applying irrigation water fertilizer
The irrigation water and fertilizer adopts a hose drip irrigation method, and the irrigation quantity of the water and fertilizer at one time is 7-15 cubic meters per mu.
The invention provides an application of an irrigation method for repairing the microbial flora of continuous cropping soil of melon crops in increasing the richness and population diversity of bacteria and fungi.
The invention also provides application of the irrigation method for repairing the microbial flora in the continuous cropping soil of the melon crops in improving the number of female flowers of main vines and branches of melons.
The invention has the beneficial effects that:
(1) the method is used for long-term continuous repair of continuous cropping soil, and the microecology of the soil under the action of autotoxic substances of plant roots is repaired by long-term irrigation of water and fertilizer containing probiotics; the quantity of soil probiotics is continuously supplemented, harmful bacteria such as fusarium wilt and the like are inhibited, and meanwhile, a bacteria source cardinal number is provided for the planting and growth of the beneficial bacteria.
(2) According to the method, during the fermentation of the seaweed fertilizer, the trace amount of the sea oil in the irrigation water, the probiotics Burkholderia and the seaweed fertilizer, the seaweed polysaccharide and the probiotics form a chelating system, and after the seaweed fertilizer is soaked and released by water, the seaweed polysaccharide and the probiotics can be applied for a long time to restore the root system, so that the root nourishing and protecting effect is achieved, meanwhile, a beneficial environment is provided for population restoration of the probiotics, and the number of main vines and female vines of melon crop plants is increased.
(3) The system is simple to operate, the bacterium bags and the loaded particles can be repeatedly used, no chemical substance exists, the water and fertilizer permeation system is simplified to the maximum extent by the water and fertilizer preparation device, the biological agent gradually falls and is diluted into irrigation water and fertilizer through the gravity of water, the sustainable prevention and control of soil treatment are facilitated, and the social benefit and the ecological benefit are great.
Drawings
FIG. 1 is a schematic view of the overall structure of a liquid manure preparation device;
FIG. 2 is a schematic structural view of a water and fertilizer collecting box;
FIG. 3 is a schematic structural view of a water and fertilizer penetration inserting plate;
FIG. 4 is a cross-sectional view of a water and fertilizer penetration inserting plate and a fungus bag;
in fig. 1 to 4, specific names of the respective structures are: the water and fertilizer spraying machine comprises a water and fertilizer collecting box 1, a water tank 1-1, a water tank front side 1-2, horizontal supporting plates 1-3, two sides of the water tank 1-4, grooved rails 1-5, a water tank rear side 1-6, water and fertilizer penetrating inserting plates 1-7, screen cylinders 1-8, screen holes 1-9, a water outlet 1-10, a water and fertilizer diluting box 2, a water inlet 2-1, a water filling port 2-2, a water outlet 2-3, a fungus bag 3 and carrier particles 3-1.
Detailed Description
Example 1 irrigation method for repairing microbial flora in soil of continuous cropping of melon crops
(1) Preparation of biological agent
The biological agent is fermentation liquor of Burkholderia, wherein Burkholderia (Burkholderia phytof ormans) has a preservation unit: the general microbiological center of China Committee for culture Collection of microorganisms, the address is No. 3 of West Lu No.1 of North Chen of the Chaozhou region, Beijing, the institute of microbiology of China academy of sciences, the preservation number is CGMCC No.15326, the strain number is 20180101 Liao CK1, and the preservation date is 29 months and 1 month in 2018;
the formula of the biological agent culture medium comprises: 5g/L of tryptone, 5g/L of yeast extract, 10-30g/L of seaweed fertilizer powder and 2-5g/L of corn starch, and adjusting the pH value to be neutral; sterilizing culture medium, inoculating Burkholderia, fermenting at 28 deg.C under stirring speed of 120rpm for 5 days to obtain bacterial suspension, and adjusting the concentration of the bacterial suspension to 6.0-8.0 × 108cfu/ml; the grain diameters of the seaweed fertilizer powder and the corn starch are 150-200 meshes;
(2) mixed load particles
The particle size of the load particles 3-1 is 2.5-6mm, the load particles 3-1 are any one of ceramic carrier particles, silica gel carrier particles and activated carbon carrier particles, the load particles 3-1 are filled into a bacterium bag and then are sealed and sterilized, the bacterium bag 3 is placed into a biological bacterium agent to be horizontally vibrated and cultured, the biological bacterium agent immerses the load particles 3-1 in the bacterium bag, the reciprocating vibration is 50-70 times/minute, the stroke is 8-12cm, the load time at 4-10 ℃ is 2-3 days, redundant bacterium liquid is removed, the load particle bacterium bag 3 for loading bacterial fertilizer is obtained, and the load particle bacterium bag 3 is stored for later use at 4 ℃;
the biological fungus bag 3 is a cylindrical mesh bag, the aperture of the fungus bag 3 is 1-2mm, the diameter of the fungus bag 3 is 7-9cm, and carrier particles 3-1 loaded with probiotics are filled in the fungus bag 3;
(3) preparation of irrigation water fertilizer
The irrigation water and fertilizer is obtained by adopting a water and fertilizer preparation device, and the water and fertilizer preparation device comprises a water and fertilizer collecting box 1 and a water and fertilizer diluting box 2; the water and fertilizer collecting box comprises a cuboid water tank 1-1 body, a horizontal supporting plate 1-3 is arranged on the inner wall of the lower portion of the front side 1-2 of the water tank, two symmetrical grooved rails 1-5 are arranged on two sides 1-4 of the water tank, the bottom ends of the grooved rails 1-5 are arranged above the supporting plate 1-3, and the top ends of the grooved rails 1-5 are close to the top ends of the rear side 1-6 of the water tank; a water and fertilizer penetration inserting plate 1-7 is inserted into the two strip groove tracks 1-5, the water and fertilizer penetration inserting plate 1-7 is provided with sieve cylinders 1-8 which are arranged at intervals, the bottoms of the sieve cylinders 1-8 are closed, the tops of the sieve cylinders 1-8 are provided with openings, sieve holes 1-9 are formed in the surfaces of the sieve cylinders 1-8, the aperture of each sieve hole 1-9 is 1-1.5cm, and the diameter of each sieve cylinder is 7-10 cm; the bottom of the water tank 1-1 is provided with a water outlet 1-10; the water and fertilizer dilution box 2 is provided with a water inlet 2-1, a water injection port 2-2 and a water outlet 2-3, the water inlet 2-1 of the water and fertilizer dilution box is connected with a water outlet 1-10 of the water and fertilizer collection box, and the water inlet 2-1 of the water and fertilizer dilution box is lower than or flush with the water outlet 1-10 of the water and fertilizer collection box;
biological bacterium bags are inserted into the sieve cylinders 1-8 of the water and fertilizer penetration inserting plates 1-7, the two sides of the water and fertilizer penetration inserting plates are inserted into the water tank grooved tracks, water is injected above the water tank, and water flows into the lower part of the water and fertilizer collecting box through the water and fertilizer penetration inserting plates to obtain water and fertilizer; the water fertilizer flows into a water fertilizer dilution box, clear water is injected through a water injection port, and irrigation water fertilizer is obtained, wherein the bacterial concentration of Burkholderia in the irrigation water fertilizer is 1 multiplied by 10-1-103cfu/ml, seaweed fertilizer 0.0001-0.01 g/L;
the irrigation water and fertilizer is applied in the field by adopting a hose drip irrigation method, and the irrigation quantity of the water and fertilizer for each irrigation is 7-15 cubic meters per mu.
Example 2
Greenhouse field application test
(1) Test time: 2018.
(2) And (3) test crops: melon, variety: the shashuai sweet king (Hebei Cangzhou Chunfeng breed Co., Ltd.).
(3) The continuous cropping age limit: for 3 years.
(4) Treatment 1 the irrigation method used in example 1 for repairing microbial flora in soil for continuous cropping of melon crops, in which the concentration of the biological bacterium Burkholderia in the irrigation water fertilizer is 1 x 102-1.0×103cfu/ml, seaweed fertilizer 0.001-0.008 g/L; treatment 2 was control normal irrigation water and the test period was the entire growing season of the crop.
(5) And (5) carrying out normal cultivation and management on the rest.
(6) Influence of irrigation method for repairing microbial flora in continuous cropping soil of melon crops on content of phenolic acid in continuous cropping soil
The method for measuring the content of the phenolic acid in the root soil is continuously carried out 1 month, 2 months, 3 months and 4 months after the melon is planted, the measuring method refers to the patent application of the patent No. 201810295481.1, and the results show that the treatment 2 adopts a common irrigation method, the increase of the phenolic acid in the continuous cropping soil is relatively quick, while an irrigation system for repairing the continuous cropping obstacle of the crop is adopted, the content of the autotoxic substance in each testing stage is obviously lower than that of the control, the increase is slow month by month, and the effect of continuously reducing the content of the phenolic acid substance is achieved.
TABLE 1 different treatment soil phenolic acid content (μ g kg)-1)
(7) The different treatments of the irrigation method for repairing the microbial flora in the continuous cropping soil of the melon crops have different influences on the growth and development of the melons, and as can be seen from the table 2, the plant height of the melon plants treated by the irrigation system for repairing the continuous cropping obstacles of the crops is obviously higher than that of a blank control, and the incidence rate of the blight is obviously lower than that of the blank control.
TABLE 2 determination of plant height and incidence of melon by different treatments
(8) Influence of irrigation method for repairing microbial flora in continuous cropping soil of melon crops on abundance and diversity of soil microorganisms
And (3) carrying out microbial abundance and diversity index determination on rhizosphere soil samples treated differently in the muskmelon vine extending period and the muskmelon fruiting period, and referring to a sampling and detection method (Hui city, 2018, research on remediation effect of crop rotation and biological preparations on muskmelon continuous cropping obstacle soil).
TABLE 3 microbial abundance and diversity index (prime period) of soil samples from different treated rhizospheres
TABLE 4 microbial abundance and diversity index (fruiting period) of rhizosphere soil samples treated differently
The abundance and uniformity of sample species are generally reflected by diversity index, wherein the Chao index and ACE index are indexes reflecting the abundance of species, and the larger the index, the higher the abundance of species community is proved. The result shows that compared with the control, the melon rhizosphere soil fungus abundance processed by the irrigation method for repairing the microbial flora in the continuous cropping soil of melon crops is obviously increased, and the bacterium abundance is obviously increased. The Shannon index and Simpson index are indexes reflecting species diversity, and the larger the index is, the higher the community diversity is. The fungus diversity and the bacteria diversity of the melon rhizosphere soil processed by the irrigation method for repairing the microbial flora in the continuous cropping soil of melon crops are obviously improved.
As can be seen from tables 3 and 4, the abundance and diversity of soil microorganisms treated by the irrigation method for repairing the microbial flora in the soil for continuous cropping of melon crops are significantly higher than those of conventional treatment in the elongation period, and the abundance and diversity of soil microorganisms treated by the irrigation method for repairing the microbial flora in the soil for continuous cropping of melon crops are increased from the elongation period to the fruiting period, i.e., the abundance and diversity are increased, and the soil fertility is gradually increased, and compared with conventional treatment, the abundance and diversity of soil microorganisms are decreased, i.e., the soil fertility is decreased along with the growth of crops.
(9) Influence of irrigation method for repairing microbial flora in continuous cropping soil of melon crops on number of female flowers of melons
And (3) surveying the number of the female flowers of the main tendrils and the branches of the plants with the same number and treated by the irrigation method for repairing the microbial flora in the continuous cropping soil of the melon crops and the contrast treatment in the whole growing season, and calculating the number growth rate of the female flowers. The results shown in Table 5 show that the irrigation method for repairing the microbial flora in the continuous cropping soil of melon crops has a remarkable growth effect on the number of female flowers of melons.
TABLE 5 Effect of different treatments on the number of female flowers in melon
| Treatment of | Rate of growth of |
| Process | |
| 1 | 26.4 |
| Treatment | |
| 2 | — |
The principle of the invention is as follows:
(1) the material is environment-friendly
The invention adopts the fungus bags and the loading particles as carriers of the biological agent, the biological agent is attached to the loading particles, the burkholderia and the seaweed fertilizer can generate chelation in the pores of the loading particles to form a stable slow release effect, and after the slow release, the loading particles and the fungus bags can be circularly applied after being sterilized to achieve the effect of environmental protection.
(2) Simple operation
The water and fertilizer preparation device has the advantages that the water and fertilizer preparation method and the preparation and dilution method are simple, the operability is high, the formula of the culture medium is easy to obtain, the cost is low, and the water and fertilizer preparation device obtains irrigation water and fertilizer through dilution to carry out hose drip irrigation by adopting the principle that water gravity permeates the loaded particles.
Claims (5)
1. An irrigation method for repairing microbial floras in continuous cropping soil of melon crops is characterized by comprising the following steps: the method comprises the following steps:
(1) preparation of biological agent
The biological agent is fermentation liquor of Burkholderia, wherein Burkholderia (Burkholderia phytof ormans) has a preservation unit: the general microbiological center of China Committee for culture Collection of microorganisms, the address is No. 3 of West Lu No.1 of North Chen of the Chaozhou region, Beijing, the institute of microbiology of China academy of sciences, the preservation number is CGMCC number 15326, the strain number is 20180101 Liao CK1, and the preservation date is 2018, 1 month and 29 days;
the formula of the biological agent culture medium comprises: 5g/L of tryptone, 5g/L of yeast extract, 10-30g/L of seaweed fertilizer powder and 2-5g/L of corn starch, and adjusting the pH value to be neutral; sterilizing culture medium, inoculating Burkholderia, fermenting at 28 deg.C under stirring speed of 120rpm for 5 days to obtain bacterial suspension, and adjusting the concentration of the bacterial suspension to 6.0-8.0 × 108cfu/ml, wherein the grain diameters of the seaweed fertilizer powder and the corn starch are 150-200 meshes;
(2) mixed load particles
The load particles are 2.5-6mm in particle size, the load particles are any one of ceramic carrier particles, silica gel carrier particles and activated carbon carrier particles, the load particles are filled into a bacterium bag and then are sealed and sterilized, the bacterium bag is placed into a biological bacterium agent to be horizontally vibrated and cultured, the biological bacterium agent immerses the load particles, the reciprocating vibration is 50-70 times/minute, the stroke is 8-12cm, the load time at 4-10 ℃ is 2-5 days, redundant bacterium liquid is drained, the load particle bacterium bag loaded with the bacterium fertilizer is obtained, and the load particle bacterium bag is stored for later use at 4 ℃;
(3) preparation of irrigation water fertilizer
Washing or soaking the bacteria bag loaded with the particles with water to release beneficial bacteria and seaweed fertilizer in the loaded particles to obtain the irrigation water fertilizer, wherein the bacteria concentration of Burkholderia in the irrigation water fertilizer is 1 × 10-1×103cfu/ml, seaweed fertilizer 0.0001-0.01 g/L;
(4) applying irrigation water fertilizer
The irrigation water and fertilizer adopts a method of carrying out hose drip irrigation in the whole growth period of the melon crops, and the irrigation quantity of the water and fertilizer at one time is 7-15 cubic meters per mu.
2. The irrigation method for remediating the microbial flora in the continuous cropping soil of melon crops as claimed in claim 1, wherein the irrigation method comprises the following steps: the irrigation water and fertilizer is obtained by adopting a water and fertilizer preparation device, and the water and fertilizer preparation device comprises a water and fertilizer collecting box and a water and fertilizer diluting box; the water and fertilizer collecting box comprises a cuboid water tank body, a horizontal supporting plate is arranged on the inner wall of the lower portion of the front side of the water tank, two symmetrical grooved tracks are arranged on two sides of the water tank, the bottom ends of the grooved tracks are arranged above the supporting plate, and the top ends of the grooved tracks are arranged at the top ends of the rear side of the water tank; a water and fertilizer penetration inserting plate is inserted between the two strip groove tracks and is provided with sieve cylinders which are arranged at intervals, the bottom of each sieve cylinder is closed, the top of each sieve cylinder is provided with an opening, and sieve holes are formed in the surfaces of the sieve cylinders; the bottom of the water tank is provided with a water outlet; the water-fertilizer diluting box is provided with a water inlet, a water filling port and a water outlet, the water inlet of the water-fertilizer diluting box is connected with the water outlet of the water-fertilizer collecting box and the water inlet of the water-fertilizer diluting box, and the water inlet of the water-fertilizer diluting box is lower than or flush with the water outlet of the water-fertilizer collecting box;
biological bacterium bags are inserted into the sieve cylinders of the water and fertilizer penetration inserting plates, the water and fertilizer penetration inserting plates are inserted into the tracks with grooves on two sides of the water tank, water is injected above the water tank, and water flows into the lower part of the water and fertilizer collecting box through the water and fertilizer penetration inserting plates to obtain water and fertilizer; the water fertilizer flows into a water fertilizer dilution box, clear water is injected through a water injection port, and irrigation water fertilizer is obtained, wherein the bacterial concentration of Burkholderia in the irrigation water fertilizer is 1 multiplied by 10-1×103cfu/ml and seaweed fertilizer 0.0001-0.01 g/L.
3. An irrigation method for continuously remediating the microbial flora in the soil for continuous cropping of melon crops as claimed in claims 1 and 2, which is characterized in that: the aperture of the cylindrical mesh bag of the biological fungus bag is 1-2mm, and the diameter of the fungus bag is 7-9 cm; the aperture of the sieve pore on the surface of the sieve cylinder is 1-1.5cm, and the diameter of the sieve cylinder is 7-10 cm.
4. The irrigation method for continuously remediating the microbial flora in the continuous cropping soil of the melon crops as claimed in claim 1, wherein the irrigation method comprises the following steps: the irrigation method is applied to increase the abundance and population diversity of bacteria and fungi in the continuous cropping soil of the melon crops.
5. The irrigation method for continuously remediating the microbial flora in the continuous cropping soil of the melon crops as claimed in claim 1, wherein the irrigation method comprises the following steps: the irrigation method is applied to increase the number of female flowers of main vines and branch vines of melon crops.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111972109A (en) * | 2020-07-16 | 2020-11-24 | 山东省土壤肥料总站 | Water and fertilizer integrated water purification system |
| CN115067045A (en) * | 2021-03-11 | 2022-09-20 | 福建省农业科学院农业生物资源研究所 | Water-fertilizer-bacterium integrated intelligent irrigation equipment and application thereof |
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