CN106588145A - Preparation method of microbial seaweed fertilizer - Google Patents
Preparation method of microbial seaweed fertilizer Download PDFInfo
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- CN106588145A CN106588145A CN201611185408.6A CN201611185408A CN106588145A CN 106588145 A CN106588145 A CN 106588145A CN 201611185408 A CN201611185408 A CN 201611185408A CN 106588145 A CN106588145 A CN 106588145A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention discloses a preparation method of a microbial seaweed fertilizer. Screened Bacillus licheniformis WS-2 having high alginate lyase activity and producing multiple ablastins is adopted to ferment press-shear assisted interaction processed seaweeds to generate a large amount of seaweed oligosaccharides and other micro-molecular special effect metabolites having a promotion effect on the growth of crops, so the bioactivity of the seaweed fertilizer is improved. The seaweed fertilizer shows the multiple crop pathogen inhibition advantage of the Bacillus licheniformis WS-2, has high nutrition added values, improves the properties of soil, and prevents the pesticide and fertilizer integration composite effects of multiple disease pests.
Description
Technical field
The present invention relates to technical field of fertilizers, more particularly, it relates to a kind of preparation method of microorganism alga fertilizer.
Background technology
Long-term a large amount of appearance using the problems such as gradually causing soil depletion, hardened, nutrient imbalance of chemical fertilizer, and agriculture
The long-term abuse of medicine also results in the serious problems such as the enhancing of pathogenic bacterium Drug resistance, pollution environment.To ensure that the high speed of agricultural can hold
Continuous Green Development, at present, the Ministry of Agriculture of China has increasingly paid attention to the use of organic fertilizer, and proposes to gradually reduce chemical fertilizer and agriculture
Usage amount of the medicine in agricultural.Sargassum be rich in multiple nutritional components, be manufacture natural organic fertilizer excellent raw material, its fermented drop
Contain natural plant growth regulator in the small molecule oligosaccharide kind Dissolve things inside of level, with very high physiologically active, promote photosynthetic
Effect, adjusts the balance of endogenous hormones.The alduronic acid glycoside material that alga fertilizer contains, energy improved soil character improves soil and protects
Outlet capacity, improves soil aggregate, stimulates root system of plant and beneficial microorganism growth.However, filling currently with microorganism
Distribution ferment Sargassum is also had difficulties with effectively discharging its nutrient substance, because containing the polysaccharide for being difficult to be decomposed in a large number in Sargassum
The salt of material and high concentration.At this stage, fermentation method prepares alga fertilizer and also there are many defects, and one to be a lack of efficient degradation Sargassum more
Efficient bacterium sugared and that bacterial manure can be directly used as；Two is that fermented bacterium is single, lacks the synergism between multi-cultur es, it is difficult to have
Macromolecular substances in effect degraded Sargassum, affect the application effect of alga fertilizer；Three is, to ensure ferment effect, to lack to Sargassum raw material
Effective preconditioning technique, traditional acid-base method processes Sargassum, can destroy nutrient in Sargassum, and to environment.
The content of the invention
The present invention prepares the deficiency of marine alga fertilizer for conventional chemical fertilizers in prior art and common fermentation method, using sieve
Two plants for choosing can produce the fermentation of bacillus of multiple beneficial metabolite and prepare complex microorganism marine alga fertilizer, help through micro-cutting
After technical finesse, the nutritional labeling in Sargassum raw material can be easier to the utilization and degraded of microorganism by fully release, greatly be carried
The high quality of marine alga fertilizer.And the extensive bacteriostatic activities of beneficial microbe Bacillus licheniformis WS-2 contained in fertilizer can
To prevent and treat the multiple diseases problem of crops to a certain extent, the use of pesticide is reduced, play the composite efficacy of " combination of pesticide and fertilizer ".
Compared with prior art, advantage and good effect of the invention are：Present invention screening from sea mud is obtained can be with height
The Bacillus licheniformis WS-2 of effect degraded Sargassum polysaccharides, this bacterial strain contains abundant glycosidase activity and proteinase activity, can have
Effect is fermented to Sargassum, produces the small molecule brown alga oligose class material that high concentration is conducive to plant growing.Micro-cutting helps technical office
The Sargassum superfine powder managed improves the release of nutrient substance, then Jing after compound strain fermentation, greatly enhances marine alga fertilizer
Fertilizer efficiency.Additionally, Bacillus licheniformis WS-2 has extensive antimicrobial spectrum, plurality of plant diseases can be to a certain extent prevented and treated, be reduced
The use of pesticide, plays the composite efficacy of " combination of pesticide and fertilizer ".
The present invention can increase soil fertility, and be conducive to Zinc fractions crumb structure so as to improved soil character, promote plant
Seed is sprouted and is grown, and strengthens plant stress-resistance ability, and safety and environmental protection improves product quality, makes product reach green or organic mark
It is accurate.
The microorganism alga fertilizer liquid fertilizer that the present invention is provided can be done directly on plant, containing active Bacillus licheniformis
The Sargassum bacterial manure of WS-2 can be used for soil base manure or production compound fertilizer.
Preservation explanation
Bacillus licheniformis WS-2 according to the present invention, in July 22 in Chinese microorganism strain preservation conservator
Can common micro-organisms center preservation (abbreviation CGMCC；Address：Institute of Microorganism, Academia Sinica；Postcode 100101), preservation is compiled
Number be CGMCC No.12813.
Specific embodiment
Technical solution of the present invention is as follows：
One bacillus licheniformis WS-2, culture presevation is numbered and is：CGMCC No.12813, Gram-positive, bar
Shape, bacterium colony rough surface has protuberance, and edge is irregular, degradable Sargassum polysaccharides, the bacterium colony shape on Algin selective medium
State is white, circular, neat in edge, and the most suitable growth pH is 7.0～7.5, and 25～35 DEG C of optimum growth temperature secretes various glucosides
Enzyme and protease, with extensive bacteriostatic activity.
Bacillus licheniformis WS-2 is taken, after activation, in being inoculated in algae culture base, semisolid fermentation culture is fermented
Liquid.In 4 DEG C, supernatant is collected in 1000g centrifugations to fermentation liquid.Supernatant collects filter liquor with 0.22 μm of membrane filtration, obtains sea
Algae Liquid Fertilizer, rich in brown alga oligose.And the alga fertilizer solid content containing active Bacillus licheniformis WS-2 after being centrifuged, low temperature baking
It is dry, Sargassum solid fertilizer is obtained, for the production of Chemical Mixed Fertilizer.
Wherein, the activated acquisitions of the Bacillus licheniformis WS-2.The culture medium of activated spawn：Cross the Kelp Powder of 80 mesh sieves
1%, peptone 0.5%, NaCl2%, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride
0.01%, distilled water configuration, 121 DEG C of Jing sterilizes 20 minutes.
And the algae culture base main component for being used to prepare fermentation liquid is that the sea of interaction technical finesse is helped through micro-cutting
Band leftover pieces superfine powder, is obtained by following steps：
1) pretreatment Thallus Laminariae (Thallus Eckloniae) raw material, drying is not higher than 10% to water content, and coarse powder is broken to 40～60 mesh；
2) add the sodium alginate that mass fraction is 1～2% as auxiliary agent, be placed in blender and fully mix；
3) grind the ultra small scale manufacture effect for producing using high energy vibration to process raw material in 2), process time is 5～30 points
Clock, obtains the micro-cutting ultra micro Thallus Laminariae (Thallus Eckloniae) powder raw material of 600～800 mesh.
The preferred preparation method of algae culture base：Micro-cutting help the Kelp Powder 3～5% of technical finesse, carbamide 0.1～0.3%,
NaCl 2～2.5%, sucrose 0.1～0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01% distill
Water is configured, and is used Jing after 121 DEG C, sterilizing in 20 minutes.Fermentation liquid is prepared using the preferred algae culture base, its fermentation condition
For：25～37 DEG C of fermentation temperature, ferment initial pH 7.5,200r min-172～108h of fermentation.
Jing above-mentioned steps, oligosaccharide final concentration in the obtained liquid seaweed manure>500mg L-1；In the Sargassum solid fertilizer
Living bacteria count>7×108CFU g-1, the content of organic matter>30%.
Embodiment 1
1. a bacillus licheniformis WS-2, Gram-positive is shaft-like, and bacterium colony rough surface has protuberance, and edge is not
Rule, degradable Sargassum polysaccharides secrete various glycosidase and protease, with extensive bacteriostatic activity.
2. after the bacillus cereuss of step 1 are activated, in being inoculated in algae culture base, semisolid fermentation culture is fermented
Liquid.
3., by the fermentation liquid obtained in step 2 in 4 DEG C, supernatant is collected in 1000g centrifugations.
4. supernatant in step 2 is filtered with 0.22 μm of filter membrane, collected filter liquor, obtained the liquid containing brown alga oligose
Body fertilizer, i.e. liquid seaweed manure, it is stand-by.
5. the medium component of activated spawn is in step 2：Cross the Kelp Powder 1% of 80 mesh sieves, peptone 0.5%, NaCl
2%th, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01%, distilled water configuration, 121 DEG C of Jing,
Use after sterilizing in 20 minutes.
6. algae culture base main component is that the Thallus Laminariae (Thallus Eckloniae) leftover pieces ultra micro of interaction technical finesse is helped through micro-cutting in step 2
Powder, following steps：
1) pretreatment Thallus Laminariae (Thallus Eckloniae) raw material, drying is not higher than 10% to water content, and coarse powder is broken to 40～60 mesh；
2) add the sodium alginate that mass fraction is 1%～2% as auxiliary agent, be placed in blender and fully mix；
3) grind the ultra small scale manufacture effect for producing using high energy vibration to process raw material in 2), process time is 5～30 points
Clock, obtains the micro-cutting ultra micro Thallus Laminariae (Thallus Eckloniae) powder raw material of 600～800 mesh.
7. the algae culture base in step 2 is：Micro-cutting help the Kelp Powder 3～5% of technical finesse, carbamide 0.1～0.3%,
NaCl 2～2.5%, sucrose 0.1～0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01% distill
Water is configured, and is used Jing after 121 DEG C, sterilizing in 20 minutes.
8. fermentation condition is described in step 2：25～37 DEG C of fermentation temperature, ferment initial pH 7.5,200r min-1Fermentation
72～108h.
Effect of fertilizer is tested：
Tetra- groups of A, B, C, D of test point carries out the planting experiment of Semen Tritici aestivi, Semen Maydiss and Semen sojae atricolor, per group of 100 plants of crops.A groups are soil
Earth applies the gained liquid seaweed manure group of embodiment 1, and seed immersion uses clear water；B groups are not applied fertilizer for soil, and (administration equivalent is clear for group
Water), seed immersion liquid seaweed manure；C groups are soil application liquid seaweed manure+seed immersion liquid seaweed manure group；D groups are control
Group, seed immersion and soil application use clear water.
The preparation of soil and seed：
Soil is saved backup Jing after the screening of 20 eye mesh screens.
Choose the complete Semen Tritici aestivi of full seed, shape, Semen Maydiss and soybean seed each 100, use potassium permanganate solution
(0.05%) sterilizing and saved backup after rinsed well repeatedly with water after 30min, and immerse clear water (or seaweed liquid prior to seeding
Fertilizer) 24h.
Planting experiment：Add in planting groove screening uniform and delicate moistening soil after sow, and use a certain amount of soil
Earth buries seed, and liquid seaweed manure (or clear water of equivalent) is applied around every seed.After the completion of operating above at room temperature
Sprouting experiment is carried out, certain management of watering is carried out daily.
The sprout time of statistics each group seed, it is as a result as shown in the table：
The impact effect that the fluid present invention liquid seaweed manure of table 1 is sprouted to seed
As a result show, tri- groups of A, B, C of liquid fertilizer soaked and apply compared to matched group D groups through liquid fertilizer, Semen Tritici aestivi, Semen sojae atricolor and
The germination percentage of corn seed is higher and sprout time of seed has shifted to an earlier date, especially when before sowing through liquid fertilizer soak seed and
The advantage for after planting applying the C groups of liquid fertilizer becomes apparent from.Thus can obtain, sprouting impact effect of the experimental group than matched group to seed
More preferably, illustrate that the liquid seaweed manure in the present invention has facilitation to seed sprouting.
Embodiment 2
1. a bacillus licheniformis WS-2, Gram-positive is shaft-like, and bacterium colony rough surface has protuberance, and edge is not
Rule, degradable Sargassum polysaccharides secrete various glycosidase and protease, with extensive bacteriostatic activity.
2. after the bacillus cereuss in step 1 are activated, equal proportion is inoculated in algae culture base, and semisolid fermentation culture is obtained
To fermentation liquid
3., by the fermentation liquid obtained in step 2 in 4 DEG C, 1000g centrifugations are collected after centrifugation containing active solution starch spore bar
The alga fertilizer solid content of bacterium WB 1 and bacillus subtilises ABTNL-2, oven drying at low temperature, i.e. Sargassum solid fertilizer.Pulverize and sieve, make
Powder, it is stand-by.
4. the medium component of activated spawn is in step 2：Cross the Kelp Powder 1% of 80 mesh sieves, peptone 0.5%, NaCl
2%th, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01%, distilled water configuration, 121 DEG C of Jing,
Use after sterilizing in 20 minutes.
5. algae culture base main component is that the Thallus Laminariae (Thallus Eckloniae) leftover pieces ultra micro of interaction technical finesse is helped through micro-cutting in step 2
Powder, following steps：
1) pretreatment Thallus Laminariae (Thallus Eckloniae) raw material, drying is not higher than 10% to water content, and coarse powder is broken to 60 mesh；
2) add the sodium alginate that mass fraction is 2% as auxiliary agent, be placed in blender and fully mix；
3) grind the ultra small scale manufacture effect for producing using high energy vibration to process raw material in 2), process time is 20 minutes,
Obtain the micro-cutting ultra micro Thallus Laminariae (Thallus Eckloniae) powder raw material of 600～800 mesh.
6. the algae culture base in step 2 is：Micro-cutting helps the Kelp Powder 5% of technical finesse, carbamide 0.3%, NaCl
2.5%th, sucrose 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, the configuration of the distilled water of calcium chloride 0.01%, Jing 121
DEG C, 20 minutes sterilizing after use.
7. fermentation condition is described in step 3：37 DEG C of fermentation temperature, ferment initial pH 7.5,200r min-1Fermentation
108h。
Effect of fertilizer is tested：
Test is divided to A, B two groups carries out the planting experiment of Semen Tritici aestivi, Semen Maydiss and Semen sojae atricolor, per group of 100 plants of crops.A groups are extra large to apply
Algae solid fertilizer group；B groups are matched group, and soil does not apply fertilizer (apply equivalent soil).
The preparation of soil and seed：
Soil is saved backup Jing after the screening of 20 eye mesh screens.
Choose the complete Semen Tritici aestivi of full seed, shape, Semen Maydiss and soybean seed each 100, use potassium permanganate solution
(0.05%) save backup after being rinsed well repeatedly with water after sterilization 30min, and immerse clear water 24h prior to seeding.
Planting experiment：Add in planting groove screening uniform and delicate be mixed with Sargassum solid fertilizer moistening soil (Gu
Body alga fertilizer is with 5t/hm2Apply) sow afterwards, and bury seed with a certain amount of soil.After the completion of operating above at room temperature,
Certain management of watering is carried out daily, and observation makes a record.During 15-20d, crop is in Seedling Stage.Respectively to Semen Tritici aestivi, jade
The enough soil of rice, soybean seedling soil inoculation passes pathogenic bacterium, wherein, Semen Tritici aestivi is banded sclerotial blight funguses, Semen Maydiss are head smut bacterium, big
Bean is epidemic disease bacterium.Three kinds of diseases are above crop main fungal sexually transmitted disease (STD) evil.After inoculation pathogenic bacteria, basic basic pipe in 5-10 days
Reason is constant, and observed and recorded illness strain number simultaneously calculates ratio.
The sprout time of statistics each group seed, it is as a result as shown in table 2 below：
The control effect of the solid of the present invention of table 2 and liquid alga fertilizer to crop soil-borne disease
As a result show, through applying the A groups of Sargassum solid fertilizer compared to matched group B groups, the illness strain of Semen Tritici aestivi, Semen sojae atricolor and Semen Maydiss
Number is substantially on the low side, it is meant that to having with the Common fungi such as wheat sharp eyespot, soybean blight, maize head smut soil-borne disease
The prevention effect of effect, this is a large amount of peculiar bacillus amyloliquefaciens due to containing in Sargassum solid fertilizer and bacillus subtilises pair
Pathogenic bacteria has effective inhibitory action.As shown in Table 2, the administration of solid alga fertilizer to have significantly suppress to Mycophyta pathogenic bacteria and makees
With can be applicable to the preventing and treating of agricultural production fungal disease.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, technology according to the present invention scheme and its
Inventive concept equivalent or change in addition, all should be included within the scope of the present invention.
Claims (4)
1. a kind of preparation method of microorganism alga fertilizer, it is characterised in that comprise the steps：
S1, culture fluid of the Bacillus licheniformis WS-2 Jing after fluid medium enrichment is taken, by inoculum concentration for 5～10% (v/m)
Ratio is inoculated in algae culture base, and semisolid fermentation culture obtains fermentation liquid；
S2, by S1 gained fermentation liquid in 4 DEG C, 1000g be centrifuged, collect supernatant；
S3, by S2 gained supernatant filtered with 0.22 μm of filter membrane, collect filter liquor, obtain liquid seaweed manure；
Solid content after S4, collection S2 centrifugations, oven drying at low temperature obtains Sargassum solid fertilizer.
2. the preparation method of microorganism alga fertilizer according to claim 1, it is characterised in that algae culture base described in step S1
Preparation method it is as follows：
S11, Thallus Laminariae (Thallus Eckloniae) raw material, drying is not higher than 10% to water content, is crushed to 40～60 mesh；
S12, the alginic acid for adding mass fraction 1～2%, mix；
S13, the ultra small scale manufacture effect for producing is ground using high energy vibration to Feedstock treating in S12 5～30 minutes, obtain 600～800 mesh sea
Band powder；
S14, step S13 gained Kelp Powder 3～5%, carbamide 0.1～0.3%, NaCl2～2.5%, sucrose 0.1～0.25%, sulfur
Sour magnesium 0.02%, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01%, add distilled water, mixing, 121 DEG C of Jing to sterilize 20 minutes.
3. the preparation method of complex microorganism alga fertilizer according to claim 2, it is characterised in that half described in step S 1
The condition of solid fermentation is：25～37 DEG C of fermentation temperature, ferment initial pH 7.5,200rmin-172～108h of fermentation.
4. according to the preparation method of the arbitrary microorganism alga fertilizer of claims 1 to 3, it is characterised in that the lichens spore
The activated acquisitions of bacillus WS-2；
The culture medium of activated spawn：Cross the Kelp Powder 1% of 80 mesh sieves, peptone 0.5%, NaCl2%, sucrose 0.25%, magnesium sulfate
0.02%th, potassium dihydrogen phosphate 0.05%, calcium chloride 0.01%, distilled water configuration, 121 DEG C of Jing sterilizes 20 minutes.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108059566A (en) * | 2018-01-18 | 2018-05-22 | 迪斯科科技集团（宜昌）有限公司 | A kind of poly spirulina peptide biostimulant |
| CN108440118A (en) * | 2018-05-10 | 2018-08-24 | 天津市辰圣海洋生物技术有限公司 | A kind of alga fertilizer preparation method degraded using mixed microorganism |
| CN108863533A (en) * | 2018-07-12 | 2018-11-23 | 深圳市芭田生态工程股份有限公司 | Cold-resistant microbial liquid fertilizer and preparation method thereof |
| CN111034441A (en) * | 2019-12-27 | 2020-04-21 | 沈阳农业大学 | Irrigation method for continuously repairing microbial flora in continuous cropping soil of melon crops |
| WO2020107116A1 (en) | 2018-11-28 | 2020-06-04 | Algueria Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355749A (en) * | 2014-11-11 | 2015-02-18 | 烟台嘉特生物技术有限公司 | Production method of microbial fermented seaweed fertilizer |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
-
2016
- 2016-12-20 CN CN201611185408.6A patent/CN106588145A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355749A (en) * | 2014-11-11 | 2015-02-18 | 烟台嘉特生物技术有限公司 | Production method of microbial fermented seaweed fertilizer |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108059566A (en) * | 2018-01-18 | 2018-05-22 | 迪斯科科技集团（宜昌）有限公司 | A kind of poly spirulina peptide biostimulant |
| CN108440118A (en) * | 2018-05-10 | 2018-08-24 | 天津市辰圣海洋生物技术有限公司 | A kind of alga fertilizer preparation method degraded using mixed microorganism |
| CN108863533A (en) * | 2018-07-12 | 2018-11-23 | 深圳市芭田生态工程股份有限公司 | Cold-resistant microbial liquid fertilizer and preparation method thereof |
| WO2020107116A1 (en) | 2018-11-28 | 2020-06-04 | Algueria Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
| EP3887343A4 (en) * | 2018-11-28 | 2022-08-10 | Produits Bio Sun Inc. | Process for the manufacture of a biostimulant or natural fertilizer based on fermented macroalgae, natural additives and probiotic bacteria for agriculture and horticulture |
| CN111034441A (en) * | 2019-12-27 | 2020-04-21 | 沈阳农业大学 | Irrigation method for continuously repairing microbial flora in continuous cropping soil of melon crops |
| CN111034441B (en) * | 2019-12-27 | 2022-03-01 | 沈阳农业大学 | Irrigation method for continuously repairing microbial flora in continuous cropping soil of melon crops |
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Application publication date: 20170426 |