CN107594070A - A kind of breeding method of Se-rich farm products and its application - Google Patents

A kind of breeding method of Se-rich farm products and its application Download PDF

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CN107594070A
CN107594070A CN201710816007.4A CN201710816007A CN107594070A CN 107594070 A CN107594070 A CN 107594070A CN 201710816007 A CN201710816007 A CN 201710816007A CN 107594070 A CN107594070 A CN 107594070A
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selenium
parts
enriched
fertilizer
rich
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冉际春
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Sichuan Yi Nong Food Co Ltd
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Sichuan Yi Nong Food Co Ltd
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The present invention provides a kind of breeding method of Se-rich farm products, the present invention selects the strong strain of fertility from saccharomycete, fermented in the culture medium containing selenium element, obtain yeast rich in selenium, feed or farm organic fertilizer are fermented under appropriate conditions using yeast rich in selenium, obtain being easy to the Se-enriched feedstuff or selenium-enriched fertilizer of animals and plants absorption, utilize Se-enriched feedstuff or selenium-rich auxiliary material, plant apply to spill selenium-enriched fertilizer suitable in the period of, to detoxification Se-enriched feedstuff suitable in the period of, Se-rich farm products are finally given.Breeding method provided by the invention, inorganic selenium salt solution is fermented to obtain small molecule Organic Selenium, reduce the toxicity of inorganic selenium fertilizer, it is easy to Matrix absorption, and the small molecule inorganic salts for being easy to absorb by generation plant after farm organic fertilizer fermentation, be advantageous to the absorption to selenium element and other elements of animals and plants, improve the utilization rate of selenium element, promote animal and plant growth, improve the resistance against diseases of animals and plants.

Description

A kind of breeding method of Se-rich farm products and its application
Technical field
The invention belongs to agricultural production, and in particular to a kind of breeding method of Se-rich farm products and application.
Background technology
Selenium element has the function that important in human body.Selenium has an antitumaous effect, human body selenium deficiency be susceptible to suffer from liver cancer, lung cancer, stomach cancer, The cancer of the esophagus, kidney, prostate cancer, carcinoma of urinary bladder, cervical carcinoma, leukaemia etc.；Selenium Wheat Protein, selenium are best anti-aging things Matter, if human body has lacked selenium and " will be no longer young ", it can cause to become old and weak before one's age；Selenium can strengthen body immunity；Selenium has short of money The effect of anti-harmful heavy metal, selenium deficiency easily trigger the heavy metal poisoning symptom such as lead, arsenic, cadmium；Selenium can adjust vitamin A, dimension life Plain C, vitamin E, vitamin K absorption and utilization, selenium deficiency can trigger myopia, cataract, retinopathy, fundus oculi disease, old age The diseases such as macular degeneration；Selenium has the function of the synthesis of regulatory protein matter, and selenium deficiency can trigger protein energy shortage property malnutritive, Chromosome damage；Selenium can strengthen reproductive function, and selenium deficiency can trigger ejaculation to be obstructed, and low sperm activity, deformity, conception rate occur Reduce, hysteritis incidence of disease rise etc. symptom；Because the organ of long-term storage selenium, the selenium needed for body is not present in selenium in human body The selenium of sufficient amount should constantly be obtained from diet, the balance of selenium concentration has important to the physiological function of many organs, tissue Protective effect and facilitation.
Human body itself can not synthesize selenium, and the selenium in food is only main source, therefore Se-rich farm products are extensively by the joyous of people Meet.Being mainly obtained for Se-rich farm products obtains in the cultivating process of agricultural product to its root or foliage dressing.And current Fertilizer containing selenium is not easy to be absorbed by plants, and the conversion ratio of selenium is low.
The content of the invention
In order to solve the technical problem of the above, the present invention makes selenium-enriched fertilizer using microbe fermentation method, and selenium element is turned The Organic Selenium for being easy to animals and plants absorption is turned to, is then applied to the culture and cultivation of animals and plants.
It is an object of the invention to provide a kind of breeding method of Se-rich farm products.
It is an object of the invention to provide a kind of breeding method of selenium enriched chicken.
It is an object of the invention to provide a kind of breeding method of selenium-rich pig.
It is an object of the invention to provide a kind of breeding method of selenium-rich rice.
It is an object of the invention to provide a kind of breeding method of selenium-enriched tea leaf.
It is an object of the invention to provide a kind of breeding method of selenium-rich beans.
It is an object of the invention to provide a kind of breeding method of selenium-enriched edible mushroom.
It is an object of the invention to provide a kind of breeding method of Se-rich wheat.
According to the breeding method of the Se-rich farm products of the specific embodiment of the invention, comprise the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) fertilizer or feed are prepared：Raw material is subjected to allotment and prepares culture medium, the Se-enriched yeast that inoculation step (1) obtains Bacterium solution is fermented, and prepares fertilizer or feed supplementation；
(3) cultivating process：The fertilizer or feed supplementation that step (2) is obtained are applied to the cultivation of animals and plants, obtain richness Selenium agricultural product.
According to the breeding method of the Se-rich farm products of the specific embodiment of the invention, it is preferable that in step (2), the feeding Material auxiliary material preparation process be：
(1) wheat bran 2-3 parts, corn 1-2 parts, dregs of beans 1-3 parts and peanut cake 1-2 parts are taken according to parts by weight, crushed, mixing is equal It is even, obtain the first mixture；
(2) glucose 0.1-0.3 parts, sodium selenite 0.01-0.05 parts and 100 parts of physiological saline are taken according to parts by weight, mixed Close uniformly, obtain the second mixture；
(3) the second mixture that the first mixture and step (2) obtained step (1) obtains, it is well mixed, sterilizing, Cooling, obtains culture medium, is inoculated with Se-enriched yeast bacterium solution obtained above, fermented and cultured, obtains Se-enriched feedstuff auxiliary material.
Conventionally used feed is base-material by the present invention, and using glucose as carbon source, addition sodium selenite, which is prepared, turns into solution, Se-enriched yeast bacterium solution is inoculated with, fermentation, inorganic selenium is converted into the Organic Selenium that animal easily absorbs, improves the absorptivity of selenium element.
According to the breeding method of the Se-rich farm products of the specific embodiment of the invention, it is preferable that in step (2), the fertilizer The preparation process of material is：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogenous fertilizer 2-5 parts, potassium are taken according to parts by weight Fertile 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, is inoculated with the selenium-rich that step (1) obtains in claim 1 Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer.
Inorganic selenium element is added in traditional fertilizer apply and spill in field, during plant is cultivated, the utilization of plant Rate is low, and the present invention ferments farm manure together with inorganic selenium by Se-enriched yeast bacterium solution, and farm manure is converted and easily inhaled without plant The inorganic salts of receipts, selenium element is converted into small molecule selenium, is easy to plant absorption, improve plant to farm manure and the profit of selenium element With rate, promote the growth of plant, promote the absorption of selenium element.
According to the breeding method of the Se-enriched egg of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking wheat bran 2-3 parts, corn 1-2 parts, dregs of beans 1-3 parts and peanut cake 1-2 parts according to parts by weight, crush, mixing is equal It is even, obtain the first mixture；
2. taking glucose 0.1-0.3 parts, sodium selenite 0.01-0.05 parts and 100 parts of physiological saline according to parts by weight, mix Uniformly, the second mixture is obtained；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into chicken feed, since the young age in days of chicken 30, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed to maturation, wherein the addition of the Se-enriched feedstuff auxiliary material is to be added in every kilogram of feed 50-80g, the egg of the chicken production is Se-enriched egg.
According to the breeding method of the selenium-rich pig of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking wheat bran 2-3 parts, corn 1-2 parts, dregs of beans 1-3 parts and peanut cake 1-2 parts according to parts by weight, crush, mixing is equal It is even, obtain the first mixture；
2. taking glucose 0.1-0.3 parts, sodium selenite 0.01-0.05 parts and 100 parts of physiological saline according to parts by weight, mix Uniformly, the second mixture is obtained；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into pig feed, since young 2 monthly ages of pig, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed to delivering for sale, wherein the addition of the Se-enriched feedstuff auxiliary material is to be added in every kilogram of feed 50-80g, feeding is to delivering for sale to obtain selenium-rich pig.
According to the breeding method of the selenium-rich rice of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer is prepared：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogen are taken according to parts by weight Fertile 2-5 parts, potash fertilizer 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, the selenium-rich that inoculation step (1) obtains Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before rice transplanting, the selenium-rich solid fertilizer is uniformly applied and spilt in rice field, per hectare rice Field, which is applied, spills 750-850kg；In rice plant of tillering stage and initial bloom stage, the selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, Selenium-enriched liquid fertilizer dilution is obtained, selenium-enriched liquid fertilizer dilution is sprayed on rice leaf, the sprinkling of per hectare rice 500-600kg；In Grain Filling, sprayed in selenium-enriched liquid fertilizer dilution, per hectare rice described in rice leaf sprinkling 600-750kg, rice maturation is treated, obtains selenium-rich rice.
According to the breeding method of the selenium-enriched tea leaf of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer is prepared：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogen are taken according to parts by weight Fertile 2-5 parts, potash fertilizer 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, the selenium-rich that inoculation step (1) obtains Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：The selenium-enriched liquid fertilizer solution is added into 50-100 times of weight water dilution, obtains selenium-enriched liquid fertilizer Dilution, selenium-enriched liquid fertilizer dilution is sprayed when tealeaves grows one leaf of bud, per hectare tealeaves sprinkling 70-500kg, is treated into Ripe harvesting, obtains selenium-enriched tea leaf.
According to the breeding method of the selenium-rich beans of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer is prepared：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogen are taken according to parts by weight Fertile 2-5 parts, potash fertilizer 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, the selenium-rich that inoculation step (1) obtains Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in beans Tanaka and spill the selenium-rich solid fertilizer, per hectare beans field, which is applied, spills 600-750kg；Selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, selenium-enriched liquid fertilizer dilution is obtained, is grown in beans Go out second true leaf to start, per a selenium-enriched liquid fertilizer dilution is sprayed 7-10 days, continuous sprinkling 2-3 months, treat maturation, obtain To selenium-rich beans.
According to the breeding method of the selenium-enriched edible mushroom of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer is prepared：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogen are taken according to parts by weight Fertile 2-5 parts, potash fertilizer 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, the selenium-rich that inoculation step (1) obtains Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Corncob, wheat bran and/or dregs of beans are taken, crushes, adds selenium-rich solid fertilizer, phosphate fertilizer and the potassium Fertilizer, it is well mixed, sows edible fungus species；Selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, obtains selenium-enriched liquid fertilizer Expect dilution, treat that fungi grows to the high beginnings of 1-2cm and selenium-enriched liquid fertilizer dilution was sprayed per 3-5 days, treat maturation, obtain selenium-rich Edible mushroom.
According to the breeding method of the Se-rich wheat of the specific embodiment of the invention, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4- 0.6 part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20-24h at 36-37 DEG C, The fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, tell Warm 80 0.001-0.002 parts, surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermentation are obtained Culture medium includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4- 1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer is prepared：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogen are taken according to parts by weight Fertile 2-5 parts, potash fertilizer 1-3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, the selenium-rich that inoculation step (1) obtains Yeast liquid, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in wheatland and spill the selenium-rich solid fertilizer, per hectare wheatland, which is applied, to be spilt 750-850kg；Prior to seeding, wheat seeds are soaked, containing the selenous acid that percentage by weight is 0.5-1% in the soak Sodium；Selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, selenium-enriched liquid fertilizer dilution is obtained, the seed soaked is broadcast Kind into wheatland, in jan flowering wheat and pustulation period in wheat foliage spray selenium-enriched liquid fertilizer dilution, maturation is treated, obtains richness Selenium wheat.
The breeding method of Se-rich farm products provided by the invention be equally applicable to rich selenium vegetables, rich selenium fruit, selenium-rich corn, Selenium-rich buckwheat, selenium-rich bitter buckwheat cultivation in.In the cultivating process of wherein described selenium-rich corn, before corn planting, this will be utilized The selenium-rich solid fertilizer that the method for invention obtains, which is applied, to be spilt in corn field, is started in corn growth to height for 30-35cm, and sprinkling is rich 15-20 days before selenium liquid fertilizer dilution to maturation, the process of dilution is that 50-100 times of weight is added into selenium-enriched liquid fertilizer Water, obtain the selenium-enriched liquid fertilizer.
Beneficial effects of the present invention are：
1. the present invention provides a kind of breeding method of Se-rich farm products, it is strong that the present invention selects fertility from saccharomycete Strain, fermented in the culture medium containing selenium element, obtain Se-enriched yeast bacterium solution, using Se-enriched yeast bacterium solution suitable Under the conditions of feed or farm organic fertilizer are fermented, obtain being easy to the Se-enriched feedstuff or selenium-enriched fertilizer of animals and plants absorption, utilize Se-enriched feedstuff or selenium-rich auxiliary material, plant apply to spill selenium-enriched fertilizer suitable in the period of, to detoxification suitable in the period of Se-enriched feedstuff, finally give Se-rich farm products.Breeding method provided by the invention, inorganic selenium salt solution is fermented to obtain small Molecule Organic Selenium, the toxicity of inorganic selenium fertilizer is reduced, be easy to Matrix absorption, and generation plant after farm organic fertilizer fermentation is easy to The small molecule inorganic salts of absorption, be advantageous to the absorption to selenium element and other elements of animals and plants, improve the utilization of selenium element Rate, promote animal and plant growth, improve the resistance against diseases of animals and plants.
2. the present invention provides the specific breeding method of a variety of agricultural product, to crops and moved in suitable period science Thing, which is applied, spills or feeds appropriate selenium-enriched fertilizer or Se-enriched feedstuff, the high crops of selenium element content can be obtained, in crops Solid and liquid selenium-enriched fertilizer are utilized in cultivating process simultaneously, absorptivity of the crops to selenium element is improved, in the cultivation of animal During, the Long-term Feeding Se-enriched feedstuff since the growth period of animal, improve animal body in selenium element content.
3. Se content is 0.25-0.5mg/ in the Se-enriched egg that the breeding method of Se-rich farm products provided by the invention obtains Kg, Se content is 0.1-0.2mg/kg in the pork of selenium-rich pig, and the Se content of selenium-rich rice is 0.15-0.27mg/kg, Se rich tea Se content is 1.2-3.5mg/kg in leaf, and Se content is 0.8-2.8mg/kg in selenium-rich beans, and Se content is in selenium-enriched edible mushroom 3-5.2mg/kg, Se content is 0.2-0.3mg/kg in Se-rich wheat, and Se content is 0.05-0.1mg/kg in rich selenium vegetables.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned Other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
The breeding method of Se-enriched egg, comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking 1 part of 2 parts of wheat bran, 1 part of corn, 1 part of dregs of beans and peanut cake according to parts by weight, crush, be well mixed, obtain the One mixture；
2. taking 0.01 part and 100 parts 0.1 part of glucose, sodium selenite physiological saline according to parts by weight, it is well mixed, obtains Second mixture；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into chicken feed, since the young age in days of chicken 30, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed to maturation, wherein the addition of the Se-enriched feedstuff auxiliary material is to be added in every kilogram of feed 50g, the egg of the chicken production is Se-enriched egg.
Embodiment 2
The breeding method of Se-enriched egg, comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking 2 parts of 3 parts of wheat bran, 2 parts of corn, 3 parts of dregs of beans and peanut cake according to parts by weight, crush, be well mixed, obtain the One mixture；
2. taking 0.05 part and 100 parts 0.3 part of glucose, sodium selenite physiological saline according to parts by weight, it is well mixed, obtains Second mixture；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into chicken feed, since the young age in days of chicken 30, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed to maturation, wherein the addition of the Se-enriched feedstuff auxiliary material is to be added in every kilogram of feed 80g, the egg of the chicken production is Se-enriched egg.
Embodiment 3
The breeding method of selenium-rich pig, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8-2.2%, dusty yeast 1.4%, KH2PO40.5%, peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking 1 part of 2 parts of wheat bran, 1 part of corn, 1 part of dregs of beans and peanut cake according to parts by weight, crush, be well mixed, obtain the One mixture；
2. taking 0.01 part and 100 parts 0.1 part of glucose, sodium selenite physiological saline according to parts by weight, it is well mixed, obtains Second mixture；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, Se-enriched yeast bacterium solution obtained above is inoculated with, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into pig feed, since young 2 monthly ages of pig, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed, wherein the addition of the Se-enriched feedstuff auxiliary material is to add 50g in every kilogram of feed, is fed To delivering for sale to obtain selenium-rich pig.
Embodiment 4
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) Se-enriched feedstuff is prepared：
1. taking 2 parts of 3 parts of wheat bran, 2 parts of corn, 3 parts of dregs of beans and peanut cake according to parts by weight, crush, be well mixed, obtain the One mixture；
2. taking 0.05 part and 100 parts 0.3 part of glucose, sodium selenite physiological saline according to parts by weight, it is well mixed, obtains Second mixture；
3. 2. the second mixture that 1. the first mixture and step that step is obtained obtain, being well mixed, sterilizing is cold But, culture medium is obtained, Se-enriched yeast bacterium solution obtained above is inoculated with, fermented and cultured, obtains Se-enriched feedstuff auxiliary material；
(3) cultivating process：The Se-enriched feedstuff auxiliary material is added into pig feed, since young 2 monthly ages of pig, raised daily The feed of the auxiliary material containing Se-enriched feedstuff is fed, wherein the addition of the Se-enriched feedstuff auxiliary material is to add 80g in every kilogram of feed, is fed To delivering for sale to obtain selenium-rich pig.
Embodiment 5
The breeding method of selenium-rich rice, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：20 parts of pig manure, 20 parts of chicken manure, 10 parts of dregs of beans cake, 2 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 1 part, 100 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before rice transplanting, the selenium-rich solid fertilizer is uniformly applied and spilt in rice field, per hectare rice Field, which is applied, spills 750kg；In rice plant of tillering stage and initial bloom stage, the selenium-enriched liquid fertilizer is added into 50 times of weight water dilutions, obtains selenium-rich Liquid fertilizer dilution, selenium-enriched liquid fertilizer dilution is sprayed on rice leaf, per hectare rice sprinkling 500kg；In water The rice pustulation period, in selenium-enriched liquid fertilizer dilution described in rice leaf sprinkling, per hectare rice sprinkling 600kg, rice maturation is treated, Obtain selenium-rich rice.
Embodiment 6
The breeding method of selenium-rich rice, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：30 parts of pig manure, 30 parts of chicken manure, 15 parts of dregs of beans cake, 5 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 3 parts, 150 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before rice transplanting, the selenium-rich solid fertilizer is uniformly applied and spilt in rice field, per hectare rice Field, which is applied, spills 850kg；In rice plant of tillering stage and initial bloom stage, the selenium-enriched liquid fertilizer is added into 100 times of weight water dilutions, obtains selenium-rich Liquid fertilizer dilution, selenium-enriched liquid fertilizer dilution is sprayed on rice leaf, per hectare rice sprinkling 600kg；In water The rice pustulation period, in selenium-enriched liquid fertilizer dilution described in rice leaf sprinkling, per hectare rice sprinkling 750kg, rice maturation is treated, Obtain selenium-rich rice.
Embodiment 7
The breeding method of selenium-enriched tea leaf, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：20 parts of pig manure, 20 parts of chicken manure, 10 parts of dregs of beans cake, 2 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 1 part, 100 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：The selenium-enriched liquid fertilizer solution is added into 50 times of weight water dilutions, obtains the dilution of selenium-enriched liquid fertilizer Liquid, selenium-enriched liquid fertilizer dilution is sprayed when tealeaves grows one leaf of bud, per hectare tealeaves sprinkling 70kg, treats ripe harvesting, Obtain selenium-enriched tea leaf.
Embodiment 8
The breeding method of selenium-enriched tea leaf, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：30 parts of pig manure, 30 parts of chicken manure, 15 parts of dregs of beans cake, 5 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 3 parts, 150 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：The selenium-enriched liquid fertilizer solution is added into 100 times of weight water dilutions, it is dilute to obtain selenium-enriched liquid fertilizer Liquid is released, selenium-enriched liquid fertilizer dilution is sprayed when tealeaves grows one leaf of bud, per hectare tealeaves sprinkling 500kg, treats that maturation is adopted Pluck, obtain selenium-enriched tea leaf.
Embodiment 9
The breeding method of selenium-rich beans, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：20 parts of pig manure, 20 parts of chicken manure, 10 parts of dregs of beans cake, 2 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 1 part, 100 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in beans Tanaka and spill the selenium-rich solid fertilizer, per hectare beans field, which is applied, spills 600kg；Selenium-enriched liquid fertilizer is added into 50 times of weight water dilutions, selenium-enriched liquid fertilizer dilution is obtained, second is grown in beans True leaf starts, and the selenium-enriched liquid fertilizer dilution of sprinkling in every 7 days, continuous sprinkling 2 months, treats maturation, obtains selenium-rich beans.
Embodiment 10
The breeding method of selenium-rich beans, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：30 parts of pig manure, 30 parts of chicken manure, 15 parts of dregs of beans cake, 5 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 3 parts, 150 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in beans Tanaka and spill the selenium-rich solid fertilizer, per hectare beans field, which is applied, spills 750kg；Selenium-enriched liquid fertilizer is added into 100 times of weight water dilutions, selenium-enriched liquid fertilizer dilution is obtained, second is grown in beans True leaf starts, and the selenium-enriched liquid fertilizer dilution of sprinkling in every 10 days, continuous sprinkling 3 months, treats maturation, obtains selenium-rich beans.
Embodiment 11
The breeding method of selenium-enriched edible mushroom, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：20 parts of pig manure, 20 parts of chicken manure, 10 parts of dregs of beans cake, 2 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 1 part, 100 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：According to 1:1:1 weight takes corncob, wheat bran and dregs of beans, crushes, adds the selenium-rich solid Fertilizer, phosphate fertilizer and potash fertilizer, it is well mixed, sows edible fungus species；Selenium-enriched liquid fertilizer is added into 50 times of weight water dilutions, obtains richness Selenium liquid fertilizer dilution, treat that fungi grows to every 3 days sprinkling selenium-enriched liquid fertilizer dilutions of the high beginnings of 1cm, treat maturation, obtain Selenium-enriched edible mushroom.
Embodiment 12
The breeding method of selenium-enriched edible mushroom, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：30 parts of pig manure, 30 parts of chicken manure, 15 parts of dregs of beans cake, 5 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 3 parts, 150 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Corncob is taken, crushes, adds selenium-rich solid fertilizer, phosphate fertilizer and the potash fertilizer, is well mixed, broadcasts Kind edible fungus species；Selenium-enriched liquid fertilizer is added into 100 times of weight water dilutions, selenium-enriched liquid fertilizer dilution is obtained, treats that fungi gives birth to It is long to start every 5 days sprinkling selenium-enriched liquid fertilizer dilutions to 2cm is high, maturation is treated, obtains selenium-enriched edible mushroom.
Embodiment 13
The breeding method of selenium-enriched edible mushroom, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1 part of peptone, 0.5 part of dusty yeast, agar 2 Part, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1 part of peptone, 0.5 part of dusty yeast, 0.0015 part of Tween 80 is remaining Measure as physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 24h at 32 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2%, sucrose 2%, dusty yeast 1.5%, KH2PO40.5%, peptone 1.5%, sodium selenite 0.2%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：25 parts of pig manure, 25 parts of chicken manure, 12 parts of dregs of beans cake, 4 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 2 parts, 125 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Wheat bran is taken, crushes, adds selenium-rich solid fertilizer, phosphate fertilizer and the potash fertilizer, is well mixed, sowing Edible fungus species；Selenium-enriched liquid fertilizer is added into 80 times of weight water dilutions, selenium-enriched liquid fertilizer dilution is obtained, treats that fungi grows to 2cm is high to start every 5 days sprinkling selenium-enriched liquid fertilizer dilutions, treats maturation, obtains selenium-enriched edible mushroom.
Embodiment 14
The breeding method of Se-rich wheat, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 0.8 part of peptone, 0.4 part of dusty yeast, fine jade 1.8 parts of fat, surplus is physiological saline,
20h is cultivated at 36 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 20h, the liquid at 36 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 0.8 part of peptone, 0.4 part of dusty yeast, 0.001 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20h at 28 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 1.5%, sucrose 1.8%, dusty yeast 1.4%, KH2PO40.5%, egg White peptone 1%, sodium selenite 0.1%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：20 parts of pig manure, 20 parts of chicken manure, 10 parts of dregs of beans cake, 2 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 1 part, 100 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in wheatland and spill the selenium-rich solid fertilizer, per hectare wheatland, which is applied, to be spilt 750kg；Prior to seeding, wheat seeds are soaked, containing the sodium selenite that percentage by weight is 0.5% in the soak；By richness Selenium liquid fertilizer adds 50 times of weight water dilutions, obtains selenium-enriched liquid fertilizer dilution, the seed soaked is sowed into wheatland, In jan flowering wheat and pustulation period in wheat foliage spray selenium-enriched liquid fertilizer dilution, maturation is treated, obtains Se-rich wheat.
Embodiment 15
The breeding method of Se-rich wheat, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1.2 parts of peptone, 0.6 part of dusty yeast, fine jade 2.2 parts of fat, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1.2 parts of peptone, 0.6 part of dusty yeast, 0.002 part of Tween 80, Surplus is physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 30h at 35 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2.2%, sucrose 2.2%, dusty yeast 1.6%, KH2PO40.6%, egg White peptone 1.5%, sodium selenite 0.3%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：30 parts of pig manure, 30 parts of chicken manure, 15 parts of dregs of beans cake, 5 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 3 parts, 150 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：Before planting, uniformly applied in wheatland and spill the selenium-rich solid fertilizer, per hectare wheatland, which is applied, to be spilt 850kg；Prior to seeding, wheat seeds are soaked, containing the sodium selenite that percentage by weight is 1% in the soak；By selenium-rich Liquid fertilizer adds 100 times of weight water dilutions, obtains selenium-enriched liquid fertilizer dilution, the seed soaked is sowed into wheatland, In jan flowering wheat and pustulation period in wheat foliage spray selenium-enriched liquid fertilizer dilution, maturation is treated, obtains Se-rich wheat.
Embodiment 16
The breeding method of rich selenium vegetables, the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein 1 part of peptone, 0.5 part of dusty yeast, agar 2 Part, surplus is physiological saline,
24h is cultivated at 37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and cultivates 24h, the liquid at 37 DEG C Body culture medium includes the following raw material of 100 parts by weight, wherein, 1 part of peptone, 0.5 part of dusty yeast, 0.0015 part of Tween 80 is remaining Measure as physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 24h at 32 DEG C, Se-enriched yeast bacterium solution, the fermentation medium are obtained Include the raw material of following percentage by weight, wherein glucose sugar 2%, sucrose 2%, dusty yeast 1.5%, KH2PO40.5%, peptone 1.5%, sodium selenite 0.2%, surplus is physiological saline；
(2) selenium-enriched fertilizer is prepared：25 parts of pig manure, 25 parts of chicken manure, 12 parts of dregs of beans cake, 4 parts of nitrogenous fertilizer, potash fertilizer are taken according to parts by weight 2 parts, 125 parts of physiological saline, it is well mixed, sterilizes, cooling, the Se-enriched yeast bacterium solution that inoculation step (1) obtains, fermented and cultured, Filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer；
(3) cultivating process：To be applied before vegetable cultivation in field of vegetables and spill the selenium-rich solid fertilizer, per hectare, which is applied, spills 450kg, Planting vegetable, selenium-enriched liquid fertilizer is added into 80 times of weight waters, selenium-enriched liquid fertilizer dilution is obtained, second is grown in vegetables True leaf starts to spray selenium-enriched liquid fertilizer dilution every 15 days, applies to spill to blade face moistening every time, has globule drippage, spray To maturation, rich selenium vegetables are obtained.
Comparative example
Crops and animal are cultivated using traditional conventional method, is periodically applied in Tanaka and spills inorganic selenium fertilizer, obtain crops, its The crops cultivated in middle comparative example have rice, tealeaves, soybean, wheat, mushroom, Chinese cabbage；Fertilizer containing selenium element is raised Chicken obtains egg.Every kind of selenium-enriched crop that every kind of crops that comparative example is obtained obtain with method provided by the invention is distinguished Extract 50 samples and carry out selenium element assay, averaged, the results are shown in Table 1.
The crops selenium element content balance result of table 1
From the results shown in Table 1, the agricultural product obtained using the breeding method of Se-rich farm products provided by the invention Middle Se content is much larger than the agricultural product that comparative example obtains.Method provided by the invention can effectively improve animals and plants to selenium element Absorptivity and conversion ratio.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.

Claims (10)

1. a kind of breeding method of Se-rich farm products, it is characterised in that the described method comprises the following steps：
(1) Se-enriched yeast bacterium solution liquid is prepared：
1. taking yeast seeds, saccharomycete is seeded on the solid medium to sterilize and cultivated,
The solid medium includes the following raw material of 100 parts by weight, wherein peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 Part, agar 1.8-2.2 parts, surplus is physiological saline,
20-24h is cultivated at 36-37 DEG C,
2. selecting the good bacterium colony of growth conditions, it is seeded to the fluid nutrient medium to sterilize and 20-24h is cultivated at 36-37 DEG C, it is described Fluid nutrient medium includes the following raw material of 100 parts by weight, wherein, peptone 0.8-1.2 parts, dusty yeast 0.4-0.6 parts, Tween 80 0.001-0.002 parts, surplus are physiological saline,
3. the saccharomycete that Liquid Culture is obtained expands culture step by step,
4. obtained saccharomycete is carried out into fermented and cultured 20-30h at 28-35 DEG C, Se-enriched yeast bacterium solution, the fermented and cultured are obtained Base includes the raw material of following percentage by weight, wherein glucose sugar 1.5-2.2%, sucrose 1.8-2.2%, dusty yeast 1.4-1.6%, KH2PO40.5-0.6%, peptone 1-1.5%, sodium selenite 0.1-0.3%, surplus are physiological saline；
(2) selenium-enriched fertilizer or feed supplementation are prepared：Raw material is subjected to allotment and prepares culture medium, the selenium-rich that inoculation step (1) obtains Yeast liquid is fermented, and prepares fertilizer or feed supplementation；
(3) cultivating process：The fertilizer or feed supplementation that step (2) is obtained are applied to the cultivation of animals and plants, obtain selenium-rich agriculture Product.
2. the breeding method of Se-rich farm products according to claim 1, it is characterised in that in step (2), the feed is auxiliary The preparation process of material is：
(1) wheat bran 2-3 parts, corn 1-2 parts, dregs of beans 1-3 parts and peanut cake 1-2 parts are taken according to parts by weight, crushed, is well mixed, Obtain the first mixture；
(2) glucose 0.1-0.3 parts, sodium selenite 0.01-0.05 parts and 100 parts of physiological saline are taken according to parts by weight, mixing is It is even, obtain the second mixture；
(3) the second mixture that the first mixture and step (2) obtained step (1) obtains, it is well mixed, sterilizes, cooling, Culture medium is obtained, the Se-enriched yeast bacterium solution that inoculation claim 1 step (1) obtains, fermented and cultured, obtains Se-enriched feedstuff auxiliary material.
3. the breeding method of Se-rich farm products according to claim 1, it is characterised in that in step (2), the fertilizer Preparation process is：Pig manure 20-30 parts, chicken manure 20-30 parts, dregs of beans cake 10-15 parts, nitrogenous fertilizer 2-5 parts, potash fertilizer 1- are taken according to parts by weight 3 parts, physiological saline 100-150 parts, it is well mixed, sterilizes, cooling, is inoculated with the Se-enriched yeast that step (1) obtains in claim 1 Bacterium, fermented and cultured, filtering, liquid is separated, obtains selenium-enriched liquid fertilizer, residual residue is selenium-rich solid fertilizer.
4. application of the breeding method of the Se-rich farm products described in claim 2 in Se-enriched egg is cultivated, it is characterised in that institute State in cultivating process, the Se-enriched feedstuff auxiliary material is added into chicken feed, since the young age in days of chicken 30, feeding daily contains selenium-rich The feed of feed supplementation is to maturation, wherein the addition of the Se-enriched feedstuff auxiliary material is to add 50-80g in every kilogram of feed.
5. application of the breeding method of the Se-rich farm products described in claim 2 in selenium-rich pig is cultivated, it is characterised in that described In cultivating process, the Se-enriched feedstuff auxiliary material is added into pig feed, since the young monthly age of pig 2, feeding daily is raised containing selenium-rich The feed of auxiliary material is expected to delivering for sale, wherein the addition of the Se-enriched feedstuff auxiliary material is to add 50-80g in every kilogram of feed.
6. application of the breeding method of the Se-rich farm products described in claim 3 in selenium-rich rice is cultivated, it is characterised in that institute State in cultivating process, before rice transplanting, the selenium-rich solid fertilizer is uniformly applied and spilt in rice field, per hectare rice field, which is applied, spills 750-850kg；In rice plant of tillering stage and initial bloom stage, the selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, obtains richness Selenium liquid fertilizer dilution, selenium-enriched liquid fertilizer dilution is sprayed on rice leaf, per hectare rice sprinkling 500- 600kg；In Grain Filling, 600- is sprayed in selenium-enriched liquid fertilizer dilution, per hectare rice described in rice leaf sprinkling 750kg。
7. application of the breeding method of the Se-rich farm products described in claim 3 in selenium-enriched tea leaf is cultivated, it is characterised in that institute State in cultivating process, the selenium-enriched liquid fertilizer solution added into 50-100 times of weight water dilution, obtains selenium-enriched liquid fertilizer dilution, Selenium-enriched liquid fertilizer dilution, per hectare tealeaves sprinkling 70-500kg are sprayed when tealeaves grows one leaf of bud.
8. application of the breeding method of the Se-rich farm products described in claim 3 in selenium-rich beans is cultivated, it is characterised in that institute State in cultivating process, before planting, uniformly applied in beans Tanaka and spill the selenium-rich solid fertilizer, per hectare beans field, which is applied, spills 600- 750kg；Selenium-enriched liquid fertilizer is added into the dilution of 50-100 times of weight water, obtains selenium-enriched liquid fertilizer dilution, the is grown in beans Two true leaves start, and per a selenium-enriched liquid fertilizer dilution is sprayed 7-10 days, continuously spray 2-3 months.
9. application of the breeding method of the Se-rich farm products described in claim 3 in selenium-enriched edible mushroom is cultivated, it is characterised in that In the cultivating process, corncob, wheat bran and/or dregs of beans are taken, crushes, adds selenium-rich solid fertilizer, phosphate fertilizer and the potash fertilizer, is mixed Close uniform, sowing edible fungus species；Selenium-enriched liquid fertilizer is added into 50-100 times of weight water dilution, obtains the dilution of selenium-enriched liquid fertilizer Liquid, treat that fungi grows to the high beginnings of 1-2cm and selenium-enriched liquid fertilizer dilution was sprayed per 3-5 days.
10. application of the breeding method of the Se-rich farm products described in claim 3 in Se-rich wheat is cultivated, it is characterised in that In the cultivating process, before planting, uniformly applied in wheatland and spill the selenium-rich solid fertilizer, per hectare wheatland, which is applied, spills 750- 850kg；Prior to seeding, wheat seeds are soaked, containing the sodium selenite that percentage by weight is 0.5-1% in the soak；Will Selenium-enriched liquid fertilizer adds the dilution of 50-100 times of weight water, obtains selenium-enriched liquid fertilizer dilution, by the seed soaked sow to In wheatland, in jan flowering wheat and pustulation period in wheat foliage spray selenium-enriched liquid fertilizer dilution.
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109354549A (en) * 2018-12-11 2019-02-19 武汉轻工大学 A kind of dedicated selenium-rich organic inorganic complex fertilizer of grape and preparation method thereof
CN109526338A (en) * 2018-11-21 2019-03-29 孟凡胜 A kind of implantation methods improving crops selenium element content
CN109666607A (en) * 2018-12-29 2019-04-23 吴云 A kind of preparation method of biological active selenium bacterium solution, biological active selenium bacterium solution, selenium-rich rice and its application
CN110476736A (en) * 2019-04-18 2019-11-22 永春天福兴农业技术有限公司 A kind of cultivation implantation methods of selenium-rich yellow dragon fruit
CN110521518A (en) * 2019-08-26 2019-12-03 龙岩市绿欣农业发展有限公司 A kind of paddy implantation methods of selenium-rich containing SOD
CN111138215A (en) * 2020-01-17 2020-05-12 湖南诺维信生物科技有限公司 Nutrient solution for cultivating selenium-rich agricultural products
CN112745157A (en) * 2020-10-30 2021-05-04 广西壮族自治区农业科学院 Selenium-enriched biological foliar fertilizer and preparation method and application thereof

Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4530846A (en) * 1983-02-15 1985-07-23 Universal Foods Corporation Method for the production of selenium yeast
CN1309177A (en) * 2001-01-17 2001-08-22 中国科学院微生物研究所 Se-enriched high-biomass yeast and its preparing process
CN1556070A (en) * 2003-12-31 2004-12-22 南京新世纪园艺研究所 Selenium enriched liquid fertilizer and public hazard less production method of selenium enriched aqgricultural product thereof
CN101139554A (en) * 2007-09-14 2008-03-12 梁林 Solid-state fermentation preparing method for Chinese herbal medicine yeast selenium
CN101709274A (en) * 2009-11-27 2010-05-19 华南农业大学 Selenium-enriched yeast and preparation method thereof
CN101792720A (en) * 2009-05-12 2010-08-04 广州市博善生物饲料有限公司 Production method of selenium-enriched yeast culture
CN102146355A (en) * 2011-01-10 2011-08-10 四川农业大学 Method for preparing selenium-enriched yeasts by using Candida utilis
CN102181371A (en) * 2011-02-25 2011-09-14 浙江深友生物技术有限公司 Yeast and method for producing selenium-enriched yeast by utilizing yeast
CN102277306A (en) * 2011-07-22 2011-12-14 安徽泰格生物技术股份有限公司 Method for fermenting selenium-enriched yeast
CN102795908A (en) * 2012-08-13 2012-11-28 江苏省农业科学院 Biological selenium-rich fertilizer and whole course dynamic gradient method for selenium-rich purple wheat
CN103284029A (en) * 2013-06-14 2013-09-11 广州格拉姆生物科技有限公司 Selenium enriched rhodopseudomonas palustris preparation and preparation method thereof
CN103820340A (en) * 2014-03-06 2014-05-28 湖北工业大学 Selenium-enriched yeast and preparation method thereof
CN103911299A (en) * 2012-12-31 2014-07-09 安琪酵母股份有限公司 Selenium-rich yeast and preparation method thereof
CN104893994A (en) * 2015-06-09 2015-09-09 广东省农业科学院动物卫生研究所 Lodderomyces elongisporus, as well as Se-accumulating culture method and application thereof
CN105175101A (en) * 2015-08-03 2015-12-23 黑龙江省农业科学院土壤肥料与环境资源研究所 Selenium-enriched foliar fertilizer preparation method therefor and applications
CN105524856A (en) * 2015-08-11 2016-04-27 广州天壤生物科技有限公司 Production method of bio-transformed selenium preparation and production method of selenium-enriched agricultural product
CN105875000A (en) * 2014-11-03 2016-08-24 重庆市涪陵区渝杨榨菜（集团）有限公司 Production method of selenium-rich agricultural products
CN106688415A (en) * 2016-11-30 2017-05-24 杨立新 Method for cultivating selenium-zinc-enriched forest product
CN106906206A (en) * 2017-04-26 2017-06-30 中国科学院合肥物质科学研究院 A kind of Se-enriched yeast, preparation method and application
CN106947723A (en) * 2017-05-18 2017-07-14 沈阳福田生物科技有限公司 A kind of microbial bacterial agent and its application
CN106962594A (en) * 2017-04-26 2017-07-21 中国科学院合肥物质科学研究院 A kind of Selenium-enriched fermentation dregs of beans, preparation method and application
CN107032886A (en) * 2017-04-26 2017-08-11 中国科学院合肥物质科学研究院 A kind of selenium-rich natural and multi-functional foliar fertilizer and preparation method thereof
CN107058208A (en) * 2017-06-28 2017-08-18 福建省麦都食品发展有限公司 A kind of production technology of Se-enriched yeast powder

Patent Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4530846A (en) * 1983-02-15 1985-07-23 Universal Foods Corporation Method for the production of selenium yeast
CN1309177A (en) * 2001-01-17 2001-08-22 中国科学院微生物研究所 Se-enriched high-biomass yeast and its preparing process
CN1556070A (en) * 2003-12-31 2004-12-22 南京新世纪园艺研究所 Selenium enriched liquid fertilizer and public hazard less production method of selenium enriched aqgricultural product thereof
CN101139554A (en) * 2007-09-14 2008-03-12 梁林 Solid-state fermentation preparing method for Chinese herbal medicine yeast selenium
CN101792720A (en) * 2009-05-12 2010-08-04 广州市博善生物饲料有限公司 Production method of selenium-enriched yeast culture
CN101709274A (en) * 2009-11-27 2010-05-19 华南农业大学 Selenium-enriched yeast and preparation method thereof
CN102146355A (en) * 2011-01-10 2011-08-10 四川农业大学 Method for preparing selenium-enriched yeasts by using Candida utilis
CN102181371A (en) * 2011-02-25 2011-09-14 浙江深友生物技术有限公司 Yeast and method for producing selenium-enriched yeast by utilizing yeast
CN102277306A (en) * 2011-07-22 2011-12-14 安徽泰格生物技术股份有限公司 Method for fermenting selenium-enriched yeast
CN102795908A (en) * 2012-08-13 2012-11-28 江苏省农业科学院 Biological selenium-rich fertilizer and whole course dynamic gradient method for selenium-rich purple wheat
CN103911299A (en) * 2012-12-31 2014-07-09 安琪酵母股份有限公司 Selenium-rich yeast and preparation method thereof
CN103284029A (en) * 2013-06-14 2013-09-11 广州格拉姆生物科技有限公司 Selenium enriched rhodopseudomonas palustris preparation and preparation method thereof
CN103820340A (en) * 2014-03-06 2014-05-28 湖北工业大学 Selenium-enriched yeast and preparation method thereof
CN105875000A (en) * 2014-11-03 2016-08-24 重庆市涪陵区渝杨榨菜（集团）有限公司 Production method of selenium-rich agricultural products
CN104893994A (en) * 2015-06-09 2015-09-09 广东省农业科学院动物卫生研究所 Lodderomyces elongisporus, as well as Se-accumulating culture method and application thereof
CN105175101A (en) * 2015-08-03 2015-12-23 黑龙江省农业科学院土壤肥料与环境资源研究所 Selenium-enriched foliar fertilizer preparation method therefor and applications
CN105524856A (en) * 2015-08-11 2016-04-27 广州天壤生物科技有限公司 Production method of bio-transformed selenium preparation and production method of selenium-enriched agricultural product
CN106688415A (en) * 2016-11-30 2017-05-24 杨立新 Method for cultivating selenium-zinc-enriched forest product
CN106906206A (en) * 2017-04-26 2017-06-30 中国科学院合肥物质科学研究院 A kind of Se-enriched yeast, preparation method and application
CN106962594A (en) * 2017-04-26 2017-07-21 中国科学院合肥物质科学研究院 A kind of Selenium-enriched fermentation dregs of beans, preparation method and application
CN107032886A (en) * 2017-04-26 2017-08-11 中国科学院合肥物质科学研究院 A kind of selenium-rich natural and multi-functional foliar fertilizer and preparation method thereof
CN106947723A (en) * 2017-05-18 2017-07-14 沈阳福田生物科技有限公司 A kind of microbial bacterial agent and its application
CN107058208A (en) * 2017-06-28 2017-08-18 福建省麦都食品发展有限公司 A kind of production technology of Se-enriched yeast powder

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109526338A (en) * 2018-11-21 2019-03-29 孟凡胜 A kind of implantation methods improving crops selenium element content
CN109526338B (en) * 2018-11-21 2021-04-02 孟凡胜 Planting method for increasing selenium content of crops
CN109354549A (en) * 2018-12-11 2019-02-19 武汉轻工大学 A kind of dedicated selenium-rich organic inorganic complex fertilizer of grape and preparation method thereof
CN109666607A (en) * 2018-12-29 2019-04-23 吴云 A kind of preparation method of biological active selenium bacterium solution, biological active selenium bacterium solution, selenium-rich rice and its application
CN110476736A (en) * 2019-04-18 2019-11-22 永春天福兴农业技术有限公司 A kind of cultivation implantation methods of selenium-rich yellow dragon fruit
CN110521518A (en) * 2019-08-26 2019-12-03 龙岩市绿欣农业发展有限公司 A kind of paddy implantation methods of selenium-rich containing SOD
CN111138215A (en) * 2020-01-17 2020-05-12 湖南诺维信生物科技有限公司 Nutrient solution for cultivating selenium-rich agricultural products
CN111138215B (en) * 2020-01-17 2023-04-25 湖南诺维信生物科技有限公司 Nutrient solution for cultivating selenium-enriched agricultural products
CN112745157A (en) * 2020-10-30 2021-05-04 广西壮族自治区农业科学院 Selenium-enriched biological foliar fertilizer and preparation method and application thereof

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