CN103820340A - Selenium-enriched yeast and preparation method thereof - Google Patents
Selenium-enriched yeast and preparation method thereof Download PDFInfo
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- CN103820340A CN103820340A CN201410078515.3A CN201410078515A CN103820340A CN 103820340 A CN103820340 A CN 103820340A CN 201410078515 A CN201410078515 A CN 201410078515A CN 103820340 A CN103820340 A CN 103820340A
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Abstract
The invention discloses a selenium-enriched yeast and a preparation method thereof. The selenium-enriched yeast is obtained by cultivating microzyme in a selenium-enriched culture medium. The preparation method comprises the steps as follows: selenium ore is immersed in an acid liquid to dissolve element selenium; heavy metal is subjected to sedimentation treatment via a phosphoric acid slat solution, and filtration is performed; the pH value of a filtrate is regulated to 5.5-6.5; a carbon source and a nitrogen source which are necessary for the growth of the microzyme are added to obtain the selenium-enriched culture medium; the obtained selenium-enriched culture medium is sterilized; the microzyme is inoculated and cultivated; microzyme bodies are collected, frozen and dried in a vacuum condition to obtain selenium-enriched yeast. According to selenium-enriched yeast and the preparation method thereof, the selenium ore is adopted as a source of the selenium element so as to broaden the selenium source material for the selenium-enriched yeast, and improve the safety of the product.
Description
Technical field
The present invention relates to food processing technology field, in particular to a kind of food grade yeast rich in selenium and preparation method thereof.
Background technology
Yeast, a kind of microorganism of the direct amount maximum of the mankind.Yeast thalline contains rich in protein, fat, sugar and vitamin B group etc., and enzyme, coenzyme, Yeast Nucleic Acid, sterol and some metabolic intermediate products.Selenium is the necessary trace element of human body, has very important physiological function, closely related with the mankind's health.Selenium can prevent and suppress tumour, anti-ageing, the normal function that maintains cardiovascular systems, the appearance of prevention of arterial sclerosis and coronary heart disease.Scarce selenium can cause Keshan disease and large bone knot disease, also can bring out cataract, hepatopathy and Pancreas Disease etc.Yeast rich in selenium can be used as raw material, other products that need to strengthen selemium nutrition element etc. of selemium nutrition element strengthening in the raw material, nourishing reinforced food of mending selenium protective foods and medicine.
Now, focus mostly in strain domestication, screening process about the research of yeast rich in selenium both at home and abroad, and relatively less to the Source Study of selenium element.For example: China's number of applying for a patent 01100600.5 " yeast rich in selenium that contains high-biomass and production method thereof ", China's number of applying for a patent 94105548.5 " yeast rich in selenium and production technique thereof ", China's number of applying for a patent 200610066369.8 " a kind of rich selenium yeast saccharomyces cerevisiae, yeast rich in selenium product and preparation method thereof " etc., all reach the object of the rich selenium of yeast to improve the nutritive ingredient such as culture medium carbon source, nitrogenous source and resistance to selenium domestication process, do not relate to the Source Study of selenium element.
In addition, selenate, selenite are all hypertoxic inorganic salt, in large-scale production process, must have injury to a certain degree to human body.Meanwhile, the selenate, the selenite that are not converted into organoselenium completely are still difficult to avoid remaining after treatment, are also unfavorable for the production of leavened food.
Summary of the invention
The present invention is directed to the problem that above developing thought exists, by lot of experiments research is carried out in selenium element source, creatively adopt the starting material that yeath selenium ore is selenium, thereby effectively utilize selenium ore to be converted into organoselenium, safety in production, prepares yeast rich in selenium.Therefore, the object of the present invention is to provide a kind of yeast rich in selenium and preparation method thereof.
Concrete, the object of the present invention is achieved like this:
A kind of yeast rich in selenium, described yeast rich in selenium is that yeast is cultivated to gained in rich seleno culture medium, the selenium element source in described rich seleno culture medium is selenium ore.
Preferably, yeast rich in selenium as above, wherein said selenium ore is yeath selenium ore.
The invention provides the preparation method of yeast rich in selenium, the method comprises the steps: selenium ore by acid soak dissolved selenium element, heavy metal is processed in phosphate solution sedimentation, filters, and regulates filtrate pH=5.5-6.5, add the necessary Carbon and nitrogen sources of Yeast Growth to obtain rich seleno culture medium, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, vacuum lyophilization, obtains yeast rich in selenium.
Preferably, the preparation method of yeast rich in selenium as mentioned above, wherein said selenium ore is yeath selenium ore, and described acid solution is sulfurous acid solution, and described phosphate solution is sodium radio-phosphate,P-32 solution.
Further preferably, the preparation method of yeast rich in selenium specifically comprises the steps: as mentioned above
(1) choose even particle size, the yeath selenium ore of diameter between 40-100 order, being placed in concentration is 10%-20%(v/v) sulfurous acid solution soaks 24-48h, uses ultrasonic concussion simultaneously, and the selenium element accelerating in selenium ore dissolves;
(2) get sodium radio-phosphate,P-32 solution heavy metal in selenium ore is carried out to precipitation process, filter, filtrate is used lye pH adjustment=5.5-6.5;
(3) add the necessary Carbon and nitrogen sources of Yeast Growth to be mixed with substratum, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, and vacuum lyophilization to moisture is less than 15%, obtains yeast rich in selenium.
Further preferably, the preparation method of yeast rich in selenium as mentioned above, wherein the alkali lye described in step (2) is the aqueous solution of following any one or more alkali: sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, salt of wormwood, saleratus, calcium hydroxide.
Compared with prior art, the yeast rich in selenium preparation method tool the present invention relates to has the following advantages and marked improvement:
(1) adopt the source of the metastable selenium ore of composition as selenium element, widened the selenium source material for the rich selenium of yeast, be conducive to making full use of of selenium ore resources.
(2) adopt the source of selenium ore as selenium element, avoided the use of inorganic selenite, thereby improved the security of production process and yeast product.
(3) adopt the sinking agent of phosphoric acid salt as removal heavy metal element, than protein cheapness, than hydrogen sulfide safety, convenient.
(4) organoselenium transformation efficiency is high, and the selenium content of gained yeast rich in selenium is 180-260 μ g/Kg.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done to further description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
The preparation of embodiment 1 yeast rich in selenium
(1) choose even particle size, the yeath selenium ore 100g of diameter between 40-100 order, is placed in 300mL sulfurous acid solution (concentration is 15%, v/v) and soaks 24h, uses ultrasonic concussion simultaneously, accelerates selenium ore and dissolves;
(2) get 150mL sodium radio-phosphate,P-32 solution (concentration is 10%, w/v) heavy metal in selenium ore is carried out to precipitation process, filter;
(3) filtrate is adjusted to pH to 6.0 with the NaOH solution of 0.1mol/L;
(4) add 10g yeast powder, 20g peptone, 20g glucose to be mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL;
(5) substratum preparing is carried out to 121 ℃ of sterilizings, 20min;
(6) take out the nutrient solution that sterilizing completes, in nutrient solution, selenium concentration is 20 μ g/ml.The yeast in three kinds of sources of previously prepd (derive from respectively vinegar unstrained spirits, residue of soya, pickles water sample, more than continuous passage cultivated for 3 generations, after testing, do not contain selenium element in its thalline) is inoculated into respectively in nutrient solution, puts 28 ℃, 179rpm shaking table cultivation 48h;
(7) by the centrifugal 5min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) dried yeast rich in selenium is pulverized, packed with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is respectively 224 μ g/Kg, 228 μ g/Kg, 234 μ g/Kg.
The preparation of embodiment 2 yeast rich in seleniums
(1) choose even particle size, the yeath selenium ore 150g of diameter between 40-100 order, is placed in the sulfurous acid solution (concentration is 20%, v/v) of 350mL and soaks 48h, uses ultrasonic concussion simultaneously, accelerates selenium ore and dissolves;
(2) sodium radio-phosphate,P-32 solution (concentration is 15%, w/v) of getting 200mL carries out precipitation process to the heavy metal in selenium ore, filters;
(3) Na with 0.1mol/L by filtrate
2cO
3solution is adjusted pH to 6.0;
(4) add 10g analysis for soybean powder, 20g peptone, 20g sucrose to be mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL;
(5) substratum preparing is carried out to 121 ℃ of sterilizings, 20min;
(6) take out the nutrient solution that sterilizing completes, in nutrient solution, selenium concentration is 18 μ g/ml, the yeast in three kinds of sources of previously prepd (is derived from respectively to vinegar unstrained spirits, residue of soya, pickles water sample, more than continuous passage cultivated for 3 generations, after testing, in its thalline, do not contain selenium element) be inoculated into respectively in nutrient solution, put 28 ℃, 179rpm shaking table cultivation 60h;
(7) by the centrifugal 8min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) dried yeast rich in selenium is pulverized, packed with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is 198 μ g/Kg, 205 μ g/Kg, 214 μ g/Kg.
The preparation of embodiment 3 yeast rich in seleniums
(1) choose even particle size, the yeath selenium ore 200g of diameter between 40-100 order, is placed in and in the sulfurous acid solution (concentration is 20%, v/v) of 400mL, soaks 48h.Use ultrasonic concussion simultaneously, accelerate selenium ore and dissolve;
(2) sodium radio-phosphate,P-32 solution (concentration is 10%, w/v) of getting 200mL carries out precipitation process to the heavy metal in selenium ore;
(3) filtrate is adjusted to pH to 6.0 with the KOH solution of 0.1mol/L;
(4) add 10g wheat bran juice, 20g peptone, 20g Semen Maydis powder to be mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL.；
(5) substratum preparing is carried out to 121 ℃ of sterilizings, 20min;
(6) take out the nutrient solution that sterilizing completes, in nutrient solution, selenium concentration is 22 μ g/ml, three saccharomycetes in three kinds of sources of previously prepd (are derived from respectively to vinegar unstrained spirits, residue of soya, pickles water sample, more than continuous passage cultivated for 3 generations, after testing, in its thalline, do not contain selenium element) be inoculated into respectively in nutrient solution, put 28 ℃, 179rpm shaking table cultivation 72h;
(7) by the centrifugal 8min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) dried yeast rich in selenium is pulverized, packed with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is 235 μ g/Kg, 243 μ g/Kg, 228 μ g/Kg.
Comparative example 1
Adopt the source of granite porphyry selenium ore as selenium element, obtain yeast nutrient solution through processing mode in the same manner as in Example 1, under identical condition, cultivate, centrifugal, dry, pulverize, packing, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 105 μ g/Kg, 118 μ g/Kg, 98 μ g/Kg.
Comparative example 2
Adopt the source of carbonatite selenium ore as selenium element, obtain yeast nutrient solution through processing mode in the same manner as in Example 1, under identical condition, cultivate, centrifugal, dry, pulverize, packing, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 110 μ g/Kg, 102 μ g/Kg, 106 μ g/Kg.
Comparative example 3
Adopt the source of skarn selenium ore as selenium element, obtain yeast nutrient solution through processing mode in the same manner as in Example 1, under identical condition, cultivate, centrifugal, dry, pulverize, packing, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 90 μ g/Kg, 96 μ g/Kg, 85 μ g/Kg.
Comparative example 4
Adopt the source of carbon siliceous slate selenium ore as selenium element, obtain yeast nutrient solution through processing mode in the same manner as in Example 1, under identical condition, cultivate, centrifugal, dry, pulverize, packing, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 79 μ g/Kg, 104 μ g/Kg, 93 μ g/Kg.
Claims (6)
1. a yeast rich in selenium, is characterized in that: described yeast rich in selenium is that yeast is cultivated to gained in rich seleno culture medium, and the selenium element source in described rich seleno culture medium is selenium ore.
2. yeast rich in selenium according to claim 1, is characterized in that: described selenium ore is yeath selenium ore.
3. the preparation method of yeast rich in selenium according to claim 1, it is characterized in that the method comprises the steps: selenium ore by acid soak dissolved selenium element, heavy metal is processed in phosphate solution sedimentation, filters, and regulates filtrate pH=5.5-6.5, add the necessary Carbon and nitrogen sources of Yeast Growth to obtain rich seleno culture medium, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, vacuum lyophilization, obtains yeast rich in selenium.
4. the preparation method of yeast rich in selenium according to claim 3, is characterized in that: described selenium ore is yeath selenium ore, and described acid solution is sulfurous acid solution, and described phosphate solution is sodium radio-phosphate,P-32 solution.
5. the preparation method of yeast rich in selenium according to claim 4, is characterized in that the method specifically comprises the steps:
Choose even particle size, the yeath selenium ore of diameter between 40-100 order, being placed in concentration is that 10%-20% sulfurous acid solution soaks 24-48h, uses ultrasonic concussion simultaneously, the selenium element accelerating in selenium ore dissolves;
Get sodium radio-phosphate,P-32 solution heavy metal in selenium ore is carried out to precipitation process, filter, filtrate is used lye pH adjustment=5.5-6.5;
Add the necessary Carbon and nitrogen sources of Yeast Growth to be mixed with substratum, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, and vacuum lyophilization to moisture is less than 15%, obtains yeast rich in selenium.
6. the preparation method of yeast rich in selenium according to claim 5, is characterized in that: the alkali lye described in step (2) is the aqueous solution of following any one or more alkali: sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, salt of wormwood, saleratus, calcium hydroxide.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480031A (en) * | 2014-12-26 | 2015-04-01 | 山东圣琪生物有限公司 | Iodine-enriched yeast preparation method and iodine-enriched yeast prepared according to method |
| CN105331546A (en) * | 2015-11-23 | 2016-02-17 | 湖北工业大学 | Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed |
| CN107011030A (en) * | 2017-04-06 | 2017-08-04 | 安徽康坶国际化肥股份有限公司 | A kind of Rice enriched-Se paddy rice organic selenium fertilizer and preparation method |
| CN107173411A (en) * | 2017-07-10 | 2017-09-19 | 李郑松 | A kind of preparation method of nutrition Selenium-enriched fermentation biscuit |
| CN107445674A (en) * | 2017-10-11 | 2017-12-08 | 广西南宁秀珀生物科技有限公司 | Se-enriched bio fertilizer production system |
| CN107500843A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | bio-fertilizer production system |
| CN107586167A (en) * | 2017-10-11 | 2018-01-16 | 广西南宁秀珀生物科技有限公司 | Biological Selenium fertilizer manufacture system with duplicate removal metal function |
| CN107594070A (en) * | 2017-09-08 | 2018-01-19 | 四川亿客农食品有限公司 | A kind of breeding method of Se-rich farm products and its application |
| CN107652040A (en) * | 2017-10-11 | 2018-02-02 | 广西南宁秀珀生物科技有限公司 | Bio-fertilizer and preparation method thereof |
| CN107663123A (en) * | 2017-10-11 | 2018-02-06 | 广西南宁秀珀生物科技有限公司 | Se-enriched bio fertilizer and preparation method thereof |
| CN107698288A (en) * | 2017-10-11 | 2018-02-16 | 广西南宁秀珀生物科技有限公司 | Bio-fertilizer production system with duplicate removal metal function |
| CN110408552A (en) * | 2019-07-08 | 2019-11-05 | 牡丹江灵泰药业股份有限公司 | Se-enriched yeast is preparing the application in the antidote of liver renal toxicity caused by cinnabar |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888048A (en) * | 2006-07-21 | 2007-01-03 | 江南大学 | Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source |
| CN101402976A (en) * | 2008-10-22 | 2009-04-08 | 四川正圆科技有限公司 | Method for producing mineral composite yeast selenium by employing selenium-containing coal gangue |
| CN102703561A (en) * | 2012-05-26 | 2012-10-03 | 彭祚全 | Method for producing activated selenium mineral powder by microbial fermentation |
-
2014
- 2014-03-06 CN CN201410078515.3A patent/CN103820340B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888048A (en) * | 2006-07-21 | 2007-01-03 | 江南大学 | Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source |
| CN101402976A (en) * | 2008-10-22 | 2009-04-08 | 四川正圆科技有限公司 | Method for producing mineral composite yeast selenium by employing selenium-containing coal gangue |
| CN102703561A (en) * | 2012-05-26 | 2012-10-03 | 彭祚全 | Method for producing activated selenium mineral powder by microbial fermentation |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104480031A (en) * | 2014-12-26 | 2015-04-01 | 山东圣琪生物有限公司 | Iodine-enriched yeast preparation method and iodine-enriched yeast prepared according to method |
| CN105331546A (en) * | 2015-11-23 | 2016-02-17 | 湖北工业大学 | Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed |
| CN105331546B (en) * | 2015-11-23 | 2018-09-25 | 湖北工业大学 | A kind of selenium-rich Candida glabrata strain FXY-4 and its cultural method and the application as fish feed additive |
| CN107011030A (en) * | 2017-04-06 | 2017-08-04 | 安徽康坶国际化肥股份有限公司 | A kind of Rice enriched-Se paddy rice organic selenium fertilizer and preparation method |
| CN107173411A (en) * | 2017-07-10 | 2017-09-19 | 李郑松 | A kind of preparation method of nutrition Selenium-enriched fermentation biscuit |
| CN107594070A (en) * | 2017-09-08 | 2018-01-19 | 四川亿客农食品有限公司 | A kind of breeding method of Se-rich farm products and its application |
| CN107586167A (en) * | 2017-10-11 | 2018-01-16 | 广西南宁秀珀生物科技有限公司 | Biological Selenium fertilizer manufacture system with duplicate removal metal function |
| CN107500843A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | bio-fertilizer production system |
| CN107652040A (en) * | 2017-10-11 | 2018-02-02 | 广西南宁秀珀生物科技有限公司 | Bio-fertilizer and preparation method thereof |
| CN107663123A (en) * | 2017-10-11 | 2018-02-06 | 广西南宁秀珀生物科技有限公司 | Se-enriched bio fertilizer and preparation method thereof |
| CN107698288A (en) * | 2017-10-11 | 2018-02-16 | 广西南宁秀珀生物科技有限公司 | Bio-fertilizer production system with duplicate removal metal function |
| CN107445674A (en) * | 2017-10-11 | 2017-12-08 | 广西南宁秀珀生物科技有限公司 | Se-enriched bio fertilizer production system |
| CN110408552A (en) * | 2019-07-08 | 2019-11-05 | 牡丹江灵泰药业股份有限公司 | Se-enriched yeast is preparing the application in the antidote of liver renal toxicity caused by cinnabar |
| CN110408552B (en) * | 2019-07-08 | 2023-04-07 | 牡丹江灵泰药业股份有限公司 | Application of selenium-enriched yeast in preparation of detoxifying drug for liver and kidney toxicity caused by cinnabar |
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Effective date of registration: 20171122 Address after: 157005 Dongan District, Mudanjiang, Heilongjiang Province, No. 34 East Chang'an Avenue Patentee after: Mudanjiang Lingtai Pharmaceuticals Co., Ltd. Address before: 430068 Wuchang, Hubei Province, South Lake, Li Jia Dun special No. 1 Patentee before: Hubei Industry University |