CN105145115B - A kind of production method of organic Se-rich zinc-rich product - Google Patents
A kind of production method of organic Se-rich zinc-rich product Download PDFInfo
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- CN105145115B CN105145115B CN201510547354.2A CN201510547354A CN105145115B CN 105145115 B CN105145115 B CN 105145115B CN 201510547354 A CN201510547354 A CN 201510547354A CN 105145115 B CN105145115 B CN 105145115B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- Pest Control & Pesticides (AREA)
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The present invention relates to a kind of organic Se-rich zinc-rich product processes, comprise the following steps：Culture medium is prepared using sucrose, soya bean etc., and adds sodium selenite, zinc sulfate, pH natures；By the access golden mushroom of mass volume ratio 5% 10% and hericium erinaceus liquid seeds, 25 27 DEG C of temperature, air agitation, fermentation time 24 60 hours after sterilizing cooling.By the processing of cultured zymotic fluid centrifuge, mycelium is collected, organic Se-rich zinc-rich product is obtained by washing, being freeze-dried.The present invention uses golden mushroom and hericium erinaceus mixed fermentation, significantly improves the production efficiency of organic selenium product, the effect of unexpected selenium-rich and zinc-rich is achieved using golden mushroom and hericium erinaceus co-fermentation.
Description
Technical field
The present invention relates to fermentation arts, are related to a kind of production method of organic Se-rich zinc-rich product.
Background technology
Selenium (Se) is the essential trace elements of the human body, closely related with health.Selenium deficiency can cause a variety of diseases, such as make
Into a series of lesions such as muscular dystrophy, oedema, anaemia, early ageing, heart disease, hepatonecrosis, pancreas atrophy, diabetes, and lead
Cause to suffer from a variety of endemic diseases such as " Keshan disease ", " Kaschin-Beck disease ", therefore, selenium is sung the praises of " special effect element " for life by scientist.Mesh
Before, there are more than 40 national selenium deficiency or few selenium in the world, Chinese Ye You22Ge provinces and cities account for more than 72% counties and cities and lack selenium element, mend
The problem of selenium is human body generality.Confirmed from many research such as nutrition, Food Science and biochemistry：Human body is to food
In various micro- absorbing degree is relevant with its chemical state, selenium element is also the selenium element in this way, free form
It is not easy the utilization that is absorbed by the body, the biological value of inorganic states selenium element is less than the selenium element of general organic, and Organic Selenium
Biological value again be less than biological source Organic Selenium biological value.Therefore, it is straight in the existing forms of selenium in food element
Connect and affect assimilation effect of the human body to selenium, not the blindness selenium-supply of division aspect, can not solve the problems, such as human body selenium deficiency well.
Zinc is also one of metallic element to be played an important role to function of human body.Zinc is the activation of the composition or enzyme of many metalloenzyme
Agent.The enzyme relevant with zinc about 70-100 kinds.Zinc can not synthesize in human body, can only be obtained from vitro.Zinc deficiency is in China
Generally existing in crowd, baby, children, pregnant woman and women of child-bearing age's zinc-deficiency are especially serious.Supplement organic Zinc can eliminate inorganic
Side effect of the zinc to human body and the stimulation to stomach and intestine so that zinc can it is more efficient by human body, more safely absorb.
At present, the source of selenium in food is mainly animality and plant food, and needs to improve production efficiency, it is necessary to adopt
With the mode of microbial fermentation.It is and generally not strong only with a kind of edible mushroom, selenium-rich effect.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of high organic selenium-rich thalline product of production efficiency, and by force
Change selenium-rich and zinc-rich ability.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of production method of organic Se-rich zinc-rich product, comprises the following steps：
Prepare culture medium：Sucrose 20-50g, the soya bean 20-50g for being broken into powder, corn flour 300g, yeast extract 27g, dimension life
Plain C 1.5g, magnesium sulfate 8g, potassium dihydrogen phosphate 15g, calcium chloride 0.15g, 0.2g zinc sulfate is added, moisturizing 800mL is stirred, finally
Final concentration of 20-50 μ g/L sodium selenite is added, pH is natural；
By mass volume ratio 5%-10% access golden mushroom seeds and the mixed liquor of hericium erinaceus seed after sterilizing cooling
Body seed, 25-27 DEG C of temperature, air agitation, fermentation time 24-60 hours.By cultured zymotic fluid 2000-10000rpm
Centrifuge processing, collect mycelium, through washing freeze-drying obtain organic Se-rich zinc-rich product.
The beneficial effects of the invention are as follows：The production efficiency of organic selenium product is improved using edible fungus species fermentation, is used
Golden mushroom seed and hericium erinaceus seed co-fermentation achieve the effect of unexpected selenium-rich.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, in golden mushroom seed and hericium erinaceus the seed mixing liquid seed, golden mushroom seed and monkey
The ratio of the number of viable of head mushroom seed is between 1: 2 to 2: 1.
Embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
Purchased from Miyang County, it leads edible mushroom Specialty Co-operative Organization to golden mushroom seed in embodiment.Hericium erinaceus seed is purchased from Gu
Mountain legend fruits and vegetables Specialty Co-operative Organization of field county.
Embodiment 1：
Prepare culture medium：Sucrose 20g, the soya bean 20g for being broken into powder, corn flour 300g, yeast extract 27g, vitamin C
1.5g, magnesium sulfate 8g, potassium dihydrogen phosphate 15g, calcium chloride 0.15g, 0.2g zinc sulfate is added, moisturizing 800mL stirrings, is eventually adding
Final concentration of 20 μ g/L sodium selenite, pH are natural；
The mixing liquid seed of golden mushroom seed and hericium erinaceus seed is accessed after sterilizing cooling by mass volume ratio 5%
(in the mixing liquid seed of the golden mushroom seed and hericium erinaceus seed, golden mushroom seed and hericium erinaceus seed
The ratio of number of viable is 1: 2), 25 DEG C of temperature, air agitation, fermentation time 24 hours.Cultured zymotic fluid is used
2000rpm centrifuge processing, collects mycelium, and organic Se-rich zinc-rich product is obtained by washing freeze-drying.
Embodiment 2：
Prepare culture medium：Sucrose 50g, the soya bean 50g for being broken into powder, corn flour 300g, yeast extract 27g, vitamin C
1.5g, magnesium sulfate 8g, potassium dihydrogen phosphate 15g, add calcium chloride 0.15g, 0.2g zinc sulfate, moisturizing 800mL stirring, be eventually adding
Final concentration of 50 μ g/L sodium selenite, pH are natural；
After sterilizing cooling golden mushroom seed and hericium erinaceus seed mixing liquid seed are accessed by mass volume ratio 10%
(in the mixing liquid seed of the golden mushroom seed and hericium erinaceus seed, golden mushroom seed and hericium erinaceus seed
The ratio of number of viable is 2: 1), 27 DEG C of temperature, air agitation, fermentation time 60 hours.Cultured zymotic fluid is used
10000rpm centrifuge processing, collects mycelium, and organic Se-rich zinc-rich product is obtained by washing freeze-drying.
Embodiment 3：
Prepare culture medium：Sucrose 41g, the soya bean 36g for being broken into powder, corn flour 300g, yeast extract 27g, vitamin C
1.5g, magnesium sulfate 8g, potassium dihydrogen phosphate 15g, calcium chloride 0.15g, 0.2g zinc sulfate is added, moisturizing 800mL stirrings, is eventually adding
Final concentration of 43 μ g/L sodium selenite, pH are natural；
The mixing liquid seed of golden mushroom seed and hericium erinaceus seed is accessed after sterilizing cooling by mass volume ratio 7%
(in golden mushroom seed and hericium erinaceus the seed mixing liquid seed, the ratio of golden mushroom and hericium erinaceus number of viable
It is worth for 1: 1), 26 DEG C of temperature, air agitation, fermentation time 40 hours.Cultured zymotic fluid is handled with 6000 centrifuge,
Mycelium is collected, organic Se-rich zinc-rich product is obtained by washing freeze-drying.
Control group 1 is identical with the production method of embodiment 3, and unique difference is to be fermented only with asparagus.Control
Group 2 is identical with the production method of embodiment 3, and unique difference is to be fermented only with Hericium erinaceus.Control group 1, the and of control group 2
The seed liquor total viable count amount of embodiment 3 is also identical.
Take control group 1, control group 2 and embodiment 3 product carry out Determination of Selenium, every group separately sampled 10 times, ask flat
Average.As a result it is as follows：
The selenium-rich content of control group 1 is 72 μ g/kg, and the selenium-rich content of control group 2 is 108 μ g/kg, the selenium-rich of embodiment 3
The μ g/kg of content 237.
It can be seen that the effect of unexpected selenium-rich and zinc-rich is achieved using golden mushroom and hericium erinaceus co-fermentation.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (2)
1. a kind of production method of organic Se-rich zinc-rich product, it is characterised in that comprise the following steps：
Prepare culture medium：Sucrose 20-50g, the soya bean 20-50g for being broken into powder, corn flour 300g, yeast extract 27g, vitamin C
1.5g, magnesium sulfate 8g, potassium dihydrogen phosphate 15g, calcium chloride 0.15g is added, zinc sulfate 0.2g, moisturizing 800mL stirring, is eventually adding
Final concentration of 20-50 μ g/L sodium selenite, pH are natural；
By the mixing liquid kind of mass volume ratio 5%-10% access golden mushroom seeds and hericium erinaceus seed after sterilizing cooling
Son, 25-27 DEG C of temperature, air agitation, fermentation time 24-60 hours, by cultured zymotic fluid with 2000-10000rpm from
Scheming is handled, and collects mycelium, organic Se-rich zinc-rich product is obtained by washing, being freeze-dried.
2. the production method of organic Se-rich zinc-rich product as claimed in claim 1, it is characterised in that：The golden mushroom seed
In the mixing liquid seed of hericium erinaceus seed, the ratio of the number of viable of golden mushroom seed and hericium erinaceus seed is 1:
2 to 2:Between 1.
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| CN105145115B true CN105145115B (en) | 2018-02-06 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105767823A (en) * | 2016-03-16 | 2016-07-20 | 上海海勋实业有限公司 | Selenium-enriched hericium erinaceus enzyme and preparation method thereof |
| CN106213520A (en) * | 2016-07-27 | 2016-12-14 | 朱梅年 | Chlorophyll selenium-rich oral nutrient liquid and preparation method thereof |
| CN107624591A (en) * | 2017-09-26 | 2018-01-26 | 覃益祥 | A kind of method for planting of organic Se-rich soya bean |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101143002A (en) * | 2007-09-03 | 2008-03-19 | 江苏省江大绿康生物工程技术研究有限公司 | Deep liquid fermentation method for preparing selenium-rich gold needle mushroom crude polysaccharide powder |
| CN101228830A (en) * | 2008-02-22 | 2008-07-30 | 江南大学 | Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn |
| CN101560523A (en) * | 2008-04-17 | 2009-10-21 | 吴炳新 | Nutrient compound based on mixed fermented culture of five probiotics |
| CN102925527A (en) * | 2012-11-14 | 2013-02-13 | 徐州工程学院 | Method for mixing and fermenting flammulina velutipes and lucid ganoderma |
| CN103229665A (en) * | 2013-04-26 | 2013-08-07 | 汤阴县食用菌协会 | Method for producing enoki mushrooms rich in selenium and zinc |
| CN103725730A (en) * | 2013-12-27 | 2014-04-16 | 内蒙古科技大学 | Refining technology of mycelia polysaccharides of selenium-enriched hericium erinaceus |
-
2015
- 2015-08-31 CN CN201510547354.2A patent/CN105145115B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101143002A (en) * | 2007-09-03 | 2008-03-19 | 江苏省江大绿康生物工程技术研究有限公司 | Deep liquid fermentation method for preparing selenium-rich gold needle mushroom crude polysaccharide powder |
| CN101228830A (en) * | 2008-02-22 | 2008-07-30 | 江南大学 | Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn |
| CN101560523A (en) * | 2008-04-17 | 2009-10-21 | 吴炳新 | Nutrient compound based on mixed fermented culture of five probiotics |
| CN102925527A (en) * | 2012-11-14 | 2013-02-13 | 徐州工程学院 | Method for mixing and fermenting flammulina velutipes and lucid ganoderma |
| CN103229665A (en) * | 2013-04-26 | 2013-08-07 | 汤阴县食用菌协会 | Method for producing enoki mushrooms rich in selenium and zinc |
| CN103725730A (en) * | 2013-12-27 | 2014-04-16 | 内蒙古科技大学 | Refining technology of mycelia polysaccharides of selenium-enriched hericium erinaceus |
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| CB03 | Change of inventor or designer information |
Inventor after: Tang Ling Inventor after: Tang Haocheng Inventor after: Tang Jianfa Inventor after: Tang Xinmao Inventor before: Tang Jianfa Inventor before: Tang Ling |
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Effective date of registration: 20180109 Address after: No. 4, Gucheng Road, Nanning, the Guangxi Zhuang Autonomous Region, the Guangxi Zhuang Autonomous Region Applicant after: Tang Jianfa Applicant after: Tang Ling Applicant after: Tang Haocheng Address before: 541300 Guilin, Xingan City Industrial Zone District C2 Applicant before: XING'AN CHENYI INDUSTRY AND TRADE CO., LTD. Applicant before: Tang Xiang |
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