CN107788506A - Selenium-enriched hericium erinaceus powder and its production method and its purposes as selenium fortification agent - Google Patents

Selenium-enriched hericium erinaceus powder and its production method and its purposes as selenium fortification agent Download PDF

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CN107788506A
CN107788506A CN201710998018.9A CN201710998018A CN107788506A CN 107788506 A CN107788506 A CN 107788506A CN 201710998018 A CN201710998018 A CN 201710998018A CN 107788506 A CN107788506 A CN 107788506A
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selenium
hericium erinaceus
powder
enriched hericium
solid state
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王伟
黄勃
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Hefei River Biological Medicine Science And Technology Co Ltd
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Hefei River Biological Medicine Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements

Abstract

The invention discloses a kind of production method of selenium-enriched hericium erinaceus powder, including step, 1) strain cultivation, 2) bacterium solution is homogeneous, 3) solid fermentation culture, the 4) preparation of selenium-enriched hericium erinaceus powder.Selenium-enriched hericium erinaceus powder of the present invention can be used as selenium fortification agent application.The Se content scope of gained selenium-enriched hericium erinaceus powder of the invention reaches national regulations;And Organic Selenium ratio is more than 99.5%;Selenium is easily absorbed in the selenium-enriched hericium erinaceus powder of the inventive method production, is remarkably improved mouse activity of glutathione peroxidase.Strain coordinates its liquid spawn production technology in the present invention, and fruiting time foreshortens to 20 days or so;Products obtained therefrom Se content of the present invention is high, available for being used as nutrition fortifier in the general food such as milk-contained drink, rice and its product, wheat flour and its product, coarse cereal powder and its product, bread, biscuit, more extend it and apply range, the popularization of national selenium-supply is effectively realized by the use of selenium fortification agent.

Description

Selenium-enriched hericium erinaceus powder and its production method and its purposes as selenium fortification agent
Technical field
The invention belongs to biological food nutrition technique field, and in particular to a kind of selenium-enriched hericium erinaceus powder and its production method with Its purposes as selenium fortification agent.
Background technology
Selenium is a kind of important trace element, has substantial connection with more than 50 kinds of diseases such as body immunity and cancers.China 72% regional selenium deficiency, selenium deficiency crowd reach as many as 800,000,000.People are also becoming increasingly recognized that the importance of selenium element.Scholarly forecast is mended Selenium is expected to substitution and replenished the calcium as the first big micro- market.Now, compatriots' selenium-supply mode covers crowd mainly by health products Seldom.It is to popularize the inevitable direction and trend of selenium-supply that selenium fortification agent is added in food.
At present《Food enrichment uses standard》Middle regulation, it is allowed to which the selenium fortification agent being added in food has 7 Kind:Sodium selenite, sodium selenate, selenoprotein, selenide of carragheen, methylselenocysteine, Se-enriched yeast, selenium-enriched edible mushroom.Before Two kinds are inorganic seleniums, and toxicity is big, absorption difference, disabled in some countries;3-5 kinds are to belong to extraction or sintetics, miscellaneous The more Organic Selenium ratios of matter are not high;6th kind of Se-enriched yeast belongs to microorganism conversion selenium, but needs to separate Organic Selenium with inorganic selenium, And it is only permitted to add in milk-contained drink;Selenium-enriched edible mushroom is the most natural, the high-quality selenium of organic selenium content highest, it is allowed to is added Add scope wide, and wherein selenium-enriched hericium erinaceus is as the rare edible mushroom of China's tradition, wide market high by acceptance level.
Patent ZL201010190735.7 discloses a kind of selenium-enriched monkey head mushroom capsule, and Se content is in its products obtained therefrom 114.05±8.09mg/kg;And national standard《Food enrichment-selenium-enriched edible mushroom powder》(GB1903.33-2016) regulation is rich in The total Se content of selenium edible fungi nutrition hardening agent need to reach 180-400mg/kg, and Organic Selenium accounts for total selenium mass percent >=98%;Specially Product reaches far away Se content standard and undetermined Organic Selenium ratio in sharp ZL201010190735.7, it is impossible to as selenium nutrition-intensified Agent.It is about 3 months that the patent, which uses hedgehog fungus bacterial fruiting time, simultaneously, fruiting time length.
The content of the invention
The up to standard and hedgehog fungus bacterial fruiting week it is an object of the invention to provide a kind of total Se content and Organic Selenium accounting Phase short selenium-enriched hericium erinaceus powder and its production method and its purposes as selenium fortification agent.
A kind of production method of selenium-enriched hericium erinaceus powder of the present invention, comprises the steps:
1) strain cultivation:Hedgehog hydnum test tube strains are inoculated in Shake flask medium, Shaking culture based component:Glucose 2-4%, starch 1-3%, dusty yeast 1-2%, yeast extract powder 1-2%, potassium dihydrogen phosphate 0.1-0.2%, magnesium sulfate 0.1- 0.2%th, remaining is water, pH value 5-6, in 26-28 DEG C of cultivation temperature, 160-200 revs/min of rotating speed lower Shaking culture 5-7 days;So After 26-28 DEG C in fermentation tank, liquid amount 65-75%, throughput 1:It is further cultured under 0.4-0.6V/Vmin 2-4 days, the fermentation Tank medium component is the same as Shaking culture based component;
2) bacterium solution is homogeneous:Bacterium solution after step 1) is cultivated is tieed up under the homogenizer effect of 2000-2500 revs/min of rotating speed Hold 0.5-1.5 minutes;
3) solid fermentation culture:Solid state cultivation material basic components are:Cotton seed hulls 80-83%, wheat bran 15-18%, urea 1- 2%th, calcium superphosphate 1-2%, lemon acid for adjusting pH value 5-6;Sodium selenite is added into solid state cultivation material basic components, is formed solid Body planting material, water is added, form solid state cultivation culture medium, bacterium solution obtained by step 2) is then inoculated into solid state cultivation culture medium In, cultivate 18-25 days rear venting fruitings, 16-21 DEG C of fruiting temperature, fruiting humidity in 24-27 DEG C of temperature, humidity 70%-90% 90%-95%, 4-6 days fruiting cycle;
4) preparation of selenium-enriched hericium erinaceus powder:Selenium-enriched hericium erinaceus drying, the crushing that step (3) is obtained, obtain selenium-rich hedgehog hydnum Mushroom powder.
In step 3) described above, amount of water is 1-2 times of solid state cultivation material weight in solid state cultivation material, bacterium solution inoculum concentration 2%-8%.
Preferably, the production method of selenium-enriched hericium erinaceus powder of the invention, it may include following step:
1) strain cultivation:Hedgehog hydnum test tube strains are inoculated in Shake flask medium, Shaking culture based component:Glucose 3%th, starch 2%, dusty yeast 1%, yeast extract powder 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, remaining be water, pH value 5.5, in 27 DEG C of cultivation temperature, 180 revs/min of rotating speed lower Shaking culture 5-7 days;Then 27 DEG C, liquid amount in fermentation tank 70%th, throughput 1:It is further cultured under 0.5V/Vmin 2-4 days, the fermentation tank culture based component is the same as Shaking culture based component;
2) bacterium solution is homogeneous:Bacterium solution after step 1) is cultivated is tieed up under the homogenizer effect of 2200-2300 revs/min of rotating speed Hold 1 minute;
3) solid fermentation culture:Solid state cultivation material basic components are:Cotton seed hulls 82%, wheat bran 16%, excessively urea 1%, phosphorus Sour calcium 1%, lemon acid for adjusting pH value 5.5;Sodium selenite is added into solid state cultivation material basic components makes the sodium selenite content be 240mg/kg-920mg/kg, solid state cultivation material is formed, add water, form solid state cultivation culture medium, then by obtained by step 2) Bacterium solution is inoculated into solid state cultivation culture medium, and 20 days rear venting fruitings, fruiting temperature are cultivated in 25 DEG C of temperature, humidity 80%-85% 18-20 DEG C, fruiting humidity 90%-92% of degree, 5 days fruiting cycles;
4) preparation of selenium-enriched hericium erinaceus powder:Selenium-enriched hericium erinaceus drying, the crushing that step (3) is obtained, obtain selenium-rich hedgehog hydnum Mushroom powder.
In step 3) described above, amount of water is 1-1.5 times of solid state cultivation material weight in solid state cultivation material, bacterium solution inoculation Measure 4%-6%.
The selenium-enriched hericium erinaceus powder of the present invention, is that production method described above is prepared.
Selenium-enriched hericium erinaceus powder of the present invention can be used as selenium fortification agent application.
Its application can be by selenium-enriched hericium erinaceus powder be added to modulation milk powder, rice and its product, wheat flour and its product, With selenium element in supplement in coarse cereal powder and its product, bread, biscuit or milk-contained drink food, or by selenium-enriched hericium erinaceus powder As other nutrition fortifiers (such as:Selenoprotein) raw materials for production.
Material amounts percentage is weight percent unless otherwise instructed in culture medium of the present invention, plant formulation Than.Hericium erinaceus used in the present invention is strain known in the state of the art, is commercially obtained, it is generally slant tube bacterium Kind, it is purchased from edible mushroom factory or research institute, is such as purchased from Hefei and founds novel bacterial factory, Shucheng County gold the earth strain plant, Anhui Province Microbial control key lab etc..
The present invention is by strain cultivation, bacterium solution is homogeneous, solid fermentation culture, the step of drying and crushing four produce to obtain selenium-rich Hedgehog hydnum mushroom powder, production process is simple, and wherein bacterium solution is homogeneous using homogenizer and in specific high rotating speed (2000-2500 revs/min of rotating speed Clock) under maintain the short time, fully smashing to bacterium ball, bacterium ball can be so realized in the case where not destroying the eucaryotic cell structure of bacterium It is more that bacterium germination point is inoculated with after smashing, selenium-enriched hericium erinaceus fruiting time can be made to be down to 20 days or so by 2-3 months;Homogenizer rotating speed is too high Overlong time can damage to the eucaryotic cell structure of bacterium, and rotating speed is too low, is unfavorable for fully smashing for bacterium ball.Meanwhile inventor It was found that, bead is such as added during shake flask fermentation by the way of conventional broken bacterium ball, but the method is used in monkey Bacterium ball can not be but crushed in head bacterium Shaking culture;And for example stirred during ferment tank using motor, but the method is used in monkey Broken bacterium ball can not be but realized in head fermentation tank culture.
Inventor be also found, when being cultivated to Hericium erinaceus, generally gypsum can be all used in general hericium erinaceus culture material Powder substitutes the land plaster in traditional Hericium erinaceus formula in plant formulation of the present invention to adjust acid-base value with citric acid, so as to The influence that sulphur atom is combined to selenium with protein in land plaster is avoided, is more beneficial for the absorption of selenium.
The present invention adds sodium selenite 240mg/kg-920mg/kg in solid state cultivation material, makes selenium-rich hedgehog hydnum obtained by production The Se content scope of mushroom powder reaches national standard《Food enrichment-selenium-enriched edible mushroom powder》(GB1903.33-2016) defined 180mg/kg-400mg/kg;And Organic Selenium ratio is more than 99.5%;Selenium easily quilt in the selenium-enriched hericium erinaceus powder of the inventive method production Absorb, be remarkably improved mouse activity of glutathione peroxidase.Selenium be glutathione peroxidase important composition into Point, the enzyme is blood Se content important symbol thing.
Strain coordinates its liquid spawn production technology in the present invention, and fruiting time foreshortens to 20 days or so;Gained of the invention Product Se content is high, available for milk-contained drink, rice and its product, wheat flour and its product, coarse cereal powder and its product, bread, Nutrition fortifier is used as in the general foods such as biscuit, it is more extended and applies range, it is effective by the use of selenium fortification agent Realize the popularization of national selenium-supply.
Embodiment
Following embodiments are further explanations for present invention using as the explaination to the technology of the present invention content, but The present invention substantive content be not limited in described in following embodiments, one of ordinary skill in the art can with and should know appoint What simple change or replacement based on true spirit all should belong to protection domain of the presently claimed invention.
Embodiment 1
The production method of selenium-enriched hericium erinaceus powder of the present invention, comprises the steps:
1) strain cultivation:Half branch Hericium erinaceus slant tube strain (is tested purchased from Anhui Province's microbial control emphasis Room, strain number RCEF6411) it is inoculated in Shake flask medium, Shaking culture based component:Glucose 3%, starch 2%, yeast Powder 1%, yeast extract powder 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, remaining be water, pH value 5.5, in cultivation temperature 27 DEG C, 180 revs/min of rotating speed lower Shaking culture 6 days, then 27 DEG C in fermentation tank, liquid amount 70% (v/v), throughput 1: It is further cultured under 0.5V/Vmin 3 days, medium component is the same as Shaking culture based component;
2) bacterium solution is homogeneous:Bacterium solution after step 1) is cultivated maintains 1 point under the homogenizer effect of 2200 revs/min of rotating speed Clock;
3) solid fermentation culture:Solid state cultivation material basic components are:Cotton seed hulls 82%, wheat bran 16%, excessively urea 1%, phosphorus Sour calcium 1%, lemon acid for adjusting pH value 5.5;Sodium selenite is added into solid state cultivation material basic components makes the sodium selenite content be 240mg/kg-920mg/kg, solid state cultivation material is formed, add 1.3 times of amount water, form solid state cultivation culture medium, then will step Rapid 2) gained bacterium solution is inoculated into solid state cultivation culture medium, inoculum concentration 5%, is led to after 25 DEG C of temperature, humidity 80% are cultivated 20 days Wind fruiting, 19 DEG C of fruiting temperature, fruiting humidity 90%, it is normal hedgehog hydnum shape mushroom type to grow Hericium erinaceus, the cm of Hyphal length 1 Harvesting, 5 days fruiting cycles;
4) preparation of selenium-enriched hericium erinaceus powder:Selenium-enriched hericium erinaceus drying, the crushing that step (3) is obtained, obtain selenium-rich hedgehog hydnum Mushroom powder.Total Se content is 180.6~396.4mg/kg in final products obtained therefrom, and Organic Selenium ratio is 99.6%~99.8%.
Table 1
Comparative example 1
Half branch Hericium erinaceus slant tube strain (founding novel bacterial factory purchased from Hefei) is inoculated in Shake flask medium, culture medium Composition is PDA culture medium;In 27 DEG C of cultivation temperature, 180 revs/min of rotating speed lower Shaking culture 6 days, then 27 in fermentation tank DEG C, liquid amount 70% (v/v), throughput 1:It is further cultured under 0.5V/Vmin 3 days, medium component is the same as Shaking culture based component; Using conventional hericium erinaceus culture material wood chip 42%, cotton seed hull 42%, wheat bran 14%, land plaster 1%, sucrose 1%, to solid state cultivation Adding sodium selenite in material basic components makes sodium selenite content be 240mg/kg-920mg/kg, forms solid state cultivation material, then add Enter 1.3 times of amount water, form solid state cultivation culture medium, then liquid fermentation bacterium solution is inoculated into solid state cultivation culture medium, be inoculated with Amount 5%, 20 days rear venting fruitings are cultivated in 25 DEG C of temperature, humidity 80%, 19 DEG C of fruiting temperature, fruiting humidity 90%, produce institute Obtain selenium-enriched hericium erinaceus powder Se content scope 134.23mg/kg (sodium selenite addition 240mg/kg in planting material) -212.24mg/ Kg (sodium selenite addition 400mg/kg in planting material);But when addition sodium selenite amount is more than 400mg/kg in solid state cultivation material When, it is misshapen mushroom (coral mushroom, and general red) to grow Hericium erinaceus, and the fruiting cycle was at 45-60 days.
Comparative example 2
Half branch Hericium erinaceus slant tube strain (being purchased from Shucheng County gold the earth strain plant) is inoculated in Shake flask medium, trained Support based component:Glucose 2%, wheat bran 5%, peptone 2%, dusty yeast 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, its Yu Weishui, pH value 5.5;In 27 DEG C of cultivation temperature, 180 revs/min of rotating speed lower Shaking culture 6 days, then 27 DEG C in fermentation tank, Liquid amount 70% (v/v), throughput 1:It is further cultured under 0.5V/Vmin 3 days, medium component is the same as Shaking culture based component;Make With conventional hericium erinaceus culture material:Corncob 86%, wheat bran 11%, land plaster 2%, phosphate fertilizer 1%, to solid state cultivation material basic components Middle addition sodium selenite makes sodium selenite content be 240mg/kg-920mg/kg, forms solid state cultivation material, adds 1.3 times of amounts Water, solid state cultivation culture medium is formed, then liquid fermentation bacterium solution is inoculated into solid state cultivation culture medium, inoculum concentration 5%, Yu Wen 25 DEG C of degree, humidity 80% cultivate 20 days rear venting fruitings, 19 DEG C of fruiting temperature, fruiting humidity 90%, production gained selenium-rich hedgehog hydnum Mushroom powder Se content scope 120.81mg/kg (sodium selenite addition 240mg/kg in planting material) -243.42mg/kg (planting materials Middle sodium selenite addition 450mg/kg);But when addition sodium selenite amount is more than 450mg/kg in solid state cultivation material, grow monkey Head mushroom fruiting for misshapen mushroom (coral mushroom, and general red) or not, the fruiting cycle was at 50-70 days.
Comparative example 3 (still uses traditional land plaster) in solid state cultivation material
In step 3), solid state cultivation material basic components are:Cotton seed hulls 82%, wheat bran 15%, urea 1%, calcium superphosphate 1%, land plaster 1%, pH value 5.5, other techniques with embodiment 1, in final gained Hericium erinaceus product total Se content be 167.4~ 323.6mg/kg, Organic Selenium ratio are 99.2%~99.7%.Sulphur atom may have impact on suction of the Hericium erinaceus to selenium in land plaster Receive.
Application examples
Testing index glutathione peroxidase be selenium enter body after participate in form important compound, so and The important indicator of absorption of human body selenium.
1 materials and methods
4~5 week old Kun Ming mices, 18~22g of body weight, are provided by Medical University Of Anhui.60 20g of selection or so are strong The Kun Ming mice of health, it is randomly divided into 6 groups;1 group is control group, feeds low selenium basal diet;2 groups are sodium selenite group, A certain amount of sodium selenite of daily gavage on the basis of low selenium basal diet (Se content equivalent to the 5th group in daily gavage dry powder Se content);3 groups are common Hericium erinaceus group, daily a certain amount of commercially available common Hericium erinaceus of gavage on the basis of low selenium basal diet Dry powder (daily gavage dry powder amount in equivalent to the 5th group);4~6 groups are basic, normal, high selenium-enriched hericium erinaceus powder group, daily gastric infusion Equivalent to 10 times of people's recommended dose, 30 times, 100 times (adult is daily daily 0.05 gram).The marrow that breaks after 30 days puts to death mouse.It is commercially available general Logical Hericium erinaceus dry powder is purchased from Shanghai Baixin Bio-Technology Co., Ltd.;Selenium-enriched hericium erinaceus powder is product of the present invention, Se content 255.4mg/kg, Organic Selenium ratio 99.7%.
Through the bloodletting of Mouse Eye bottom, blood sample is gathered, with the anti-freezing of preprepared liquaemin (50U/ml whole bloods), warp 3500rpm, 10min is centrifuged, separated plasma, 4 DEG C stored refrigerated, to be measured.
The equal free choice feeding of small white mouse, drinking-water during experiment, cage, well-ventilated in room, receptacle temperature control exist 20 ± 3 DEG C, relative humidity is 30%~73%.Grow situation and the other abnormal conditions of small white mouse are observed and recorded daily.
1.1 basal diet
Raw material forms:Wheat 51%, Soybean Meal 15%, egg-white powder 8%, vegetable oil 3%, wheat bran 13%, cellulose 3%, calcium carbonate 2.5%, sodium dihydrogen phosphate 1.5%, lysine 1%, epsom salt 1%, trace mineral supplement (is free of selenium Compound) 0.5%, vitamin 0.5%, amount to 100%.The percentage is weight ratio.
Nutritional ingredient:Crude protein 21.488%, crude fat 4.642%, crude fibre 5.288%, coarse ash 2.572%, calcium 1.111%, phosphorus 0.717%, calcium:Phosphorus 1.548, lysine 1.364%, methionine+cystine 0.483%, magnesium 0.258%, potassium 0.686%, sodium 0.686%, iron (addition) 120.434mg/kg, manganese (addition) 76.437mg/kg, copper (addition) 10.09mg/kg, zinc (addition) 35.718mg/kg, iodine (addition) 0.535mg/kg, selenium (basal diet calculated value) 0.012mg/kg。
1.2 Testing index and method
The detection of GSH-Px activity:GSH-Px activity is represented with being catalyzed GSH reaction speed.Specific steps are according to reagent Box (building up biological Co., Ltd purchased from Nanjing) illustrates to carry out.
1.3 result
Influence of the varying level selenium-enriched hericium erinaceus powder to mouse GXH-Px activity
Influence of the selenium-enriched hericium erinaceus powder of table 2 to mouse liver and GPX3 vigor
As can be seen from Table 2,4,5,6 groups of blood plasma and liver Glutathione Peroxidase vigor pole be significantly higher than 1, 2nd, 3 groups of (P<0.01), also pole is significantly higher than 1 group of (P for 2,3 groups of blood plasma and liver Glutathione Peroxidase vigor<0.01). It can be seen that selenium-enriched hericium erinaceus powder treatment group mice plasma and liver Glutathione Peroxidase vigor improve a lot compared with control, Sodium selenite and common Hericium erinaceus dry powder treatment group also improve, but effect is not so good as selenium-enriched hericium erinaceus powder treatment group.And with The raising glutathione peroxidase activity for selenium-enriched hericium erinaceus powder gavage amount improves.

Claims (5)

1. a kind of production method of selenium-enriched hericium erinaceus powder, comprises the steps:
1) strain cultivation:Hedgehog hydnum test tube strains are inoculated in Shake flask medium, Shaking culture based component:Glucose 2- 4%th, starch 1-3%, dusty yeast 1-2%, yeast extract powder 1-2%, potassium dihydrogen phosphate 0.1-0.2%, magnesium sulfate 0.1- 0.2%th, remaining is water, pH value 5-6, in 26-28 DEG C of cultivation temperature, 160-200 revs/min of rotating speed lower Shaking culture 5-7 days;So After 26-28 DEG C in fermentation tank, liquid amount 65-75%, throughput 1:It is further cultured under 0.4-0.6V/Vmin 2-4 days, the fermentation Tank medium component is the same as Shaking culture based component;
2) bacterium solution is homogeneous:Bacterium solution after step 1) is cultivated maintains under the homogenizer effect of 2000-2500 revs/min of rotating speed 0.5-1.5 minutes;
3) solid fermentation culture:Solid state cultivation material basic components are:Cotton seed hulls 80-83%, wheat bran 15-18%, urea 1-2%, Calcium superphosphate 1-2%, lemon acid for adjusting pH value 5-6;Sodium selenite is added into solid state cultivation material basic components, solid is formed and plants Training material, adds water, forms solid state cultivation culture medium, and then bacterium solution obtained by step 2) is inoculated into solid state cultivation culture medium, 18-25 days rear venting fruitings, 16-21 DEG C of fruiting temperature, fruiting humidity are cultivated in 24-27 DEG C of temperature, humidity 70%-90% 90%-95%, 4-6 days fruiting cycle;
4) preparation of selenium-enriched hericium erinaceus powder:Selenium-enriched hericium erinaceus drying, the crushing that step (3) is obtained, obtain selenium-enriched hericium erinaceus powder.
2. the production method of selenium-enriched hericium erinaceus powder as claimed in claim 1, it is characterised in that comprise the steps:
1) strain cultivation:Hedgehog hydnum test tube strains are inoculated in Shake flask medium, Shaking culture based component:Glucose 3%, Starch 2%, dusty yeast 1%, yeast extract powder 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, remaining be water, pH value 5.5, In 27 DEG C of cultivation temperature, 180 revs/min of rotating speed lower Shaking culture 5-7 days;Then 27 DEG C in fermentation tank, it is liquid amount 70%, logical Tolerance 1:It is further cultured under 0.5V/Vmin 2-4 days, the fermentation tank culture based component is the same as Shaking culture based component;
2) bacterium solution is homogeneous:Bacterium solution after step 1) is cultivated maintains 1 under the homogenizer effect of 2200-2300 revs/min of rotating speed Minute;
3) solid fermentation culture:Solid state cultivation material basic components are:Cotton seed hulls 82%, wheat bran 16%, urea 1%, calcium superphosphate 1%, lemon acid for adjusting pH value 5.5;Sodium selenite is added into solid state cultivation material basic components makes the sodium selenite content be 240mg/kg-920mg/kg, solid state cultivation material is formed, add water, form solid state cultivation culture medium, then by obtained by step 2) Bacterium solution is inoculated into solid state cultivation culture medium, and 20 days rear venting fruitings, fruiting temperature are cultivated in 25 DEG C of temperature, humidity 80%-85% 18-20 DEG C, fruiting humidity 92%-93% of degree, 5 days fruiting cycles;
4) preparation of selenium-enriched hericium erinaceus powder:Selenium-enriched hericium erinaceus drying, the crushing that step (3) is obtained, obtain selenium-enriched hericium erinaceus powder.
3. a kind of selenium-enriched hericium erinaceus powder, it is characterised in that be prepared by the production method of claim 1 or 2.
4. purposes of the selenium-enriched hericium erinaceus powder as selenium fortification agent described in claim 3.
5. purposes as claimed in claim 4, it is characterised in that selenium-enriched hericium erinaceus powder is added to modulation milk powder, rice and its system With selenium element in supplement in product, wheat flour and its product, coarse cereal powder and its product, bread, biscuit or milk-contained drink food, Or the raw materials for production using selenium-enriched hericium erinaceus powder as other nutrition fortifiers.
CN201710998018.9A 2017-10-18 2017-10-18 Selenium-enriched hericium erinaceus powder and its production method and its purposes as selenium fortification agent Pending CN107788506A (en)

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Cited By (2)

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CN109076882A (en) * 2018-06-29 2018-12-25 江西天和食用菌开发有限公司 A kind of mycelial cultural method of selenium-enriched edible mushroom and its application
CN109076882B (en) * 2018-06-29 2021-09-03 江西天和食用菌开发有限公司 Culture method and application of selenium-rich edible fungus mycelia

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