CN103820340B - A kind of yeast rich in selenium and preparation method thereof - Google Patents

A kind of yeast rich in selenium and preparation method thereof Download PDF

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CN103820340B
CN103820340B CN201410078515.3A CN201410078515A CN103820340B CN 103820340 B CN103820340 B CN 103820340B CN 201410078515 A CN201410078515 A CN 201410078515A CN 103820340 B CN103820340 B CN 103820340B
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selenium
yeast
rich
ore
solution
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CN103820340A (en
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李冬生
汪超
祁勇刚
石勇
王金华
高冰
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Mudanjiang Lingtai Pharmaceuticals Co., Ltd.
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Hubei University of Technology
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Abstract

The invention discloses a kind of yeast rich in selenium and preparation method thereof, described yeast rich in selenium is that yeast is cultivated gained in rich seleno culture medium, and method is: by selenium ore by acid soak dissolved selenium element, phosphate solution settlement treatment heavy metal, filter, regulate filtrate pH=5.5-6.5, add the necessary Carbon and nitrogen sources of Yeast Growth and obtain rich seleno culture medium, sterilizing, inoculation yeast bacterium is cultivated, collect yeast thalline, vacuum lyophilization, obtains yeast rich in selenium.The present invention adopts selenium ore as the source of selenium element, has widened the selenium source material for the rich selenium of yeast, has improve the security of product simultaneously.

Description

A kind of yeast rich in selenium and preparation method thereof
Technical field
The present invention relates to food processing technology field, in particular to a kind of food grade yeast rich in selenium and preparation method thereof.
Background technology
Yeast, a kind of microorganism that the direct amount of the mankind is maximum.Yeast thalline contains rich in protein, fat, sugar and vitamin B group etc., and enzyme, coenzyme, Yeast Nucleic Acid, sterol and some metabolic intermediate products.Selenium is the necessary trace element of human body, has very important physiological function, closely related with the health of the mankind.Selenium can prevention and prohibition tumour, anti-ageing, maintain the normal function of cardiovascular systems, the appearance of prevention of arterial sclerosis and coronary heart disease.Scarce selenium can cause Keshan disease and large bone knot disease, also can bring out cataract, hepatopathy and Pancreas Disease etc.Yeast rich in selenium can be used as mend selenium protective foods and medicine raw material, selemium nutrition element strengthening in nourishing reinforced food raw material, other need to strengthen the product etc. of selemium nutrition element.
Now, the research both at home and abroad about yeast rich in selenium focuses mostly in strain domestication, screening process, and relatively less to the Source Study of selenium element.Such as: China's number of applying for a patent 01100600.5 " yeast rich in selenium containing high-biomass and production method thereof ", China's number of applying for a patent 94105548.5 " yeast rich in selenium and production technique thereof ", China's number of applying for a patent 200610066369.8 " a kind of rich selenium yeast saccharomyces cerevisiae, yeast rich in selenium product and preparation method thereof " etc., all to improve the object that the nutritive ingredient such as culture medium carbon source, nitrogenous source and resistance to selenium domestication process reach the rich selenium of yeast, do not relate to the Source Study of selenium element.
In addition, selenate, selenite are all hypertoxic inorganic salt, in large-scale production process, inherently have injury to a certain degree to human body.Meanwhile, be not converted into the selenate of organoselenium, selenite completely and be still difficult to avoid remaining after treatment, be also unfavorable for the production of leavened food.
Summary of the invention
The present invention is directed to above developing thought Problems existing, by carrying out lot of experiments research to selenium element source, creatively adopt yeath selenium ore to be the starting material of selenium, thus effectively utilize selenium ore to be converted into organoselenium, safety in production, prepares yeast rich in selenium.Therefore, the object of the present invention is to provide a kind of yeast rich in selenium and preparation method thereof.
Concrete, the object of the present invention is achieved like this:
A kind of yeast rich in selenium, described yeast rich in selenium is that yeast is cultivated gained in rich seleno culture medium, and the selenium element source in described rich seleno culture medium is selenium ore.
Preferably, yeast rich in selenium as above, wherein said selenium ore is yeath selenium ore.
The invention provides the preparation method of yeast rich in selenium, the method comprises the steps: selenium ore by acid soak dissolved selenium element, phosphate solution settlement treatment heavy metal, filters, and regulates filtrate pH=5.5-6.5, add the necessary Carbon and nitrogen sources of Yeast Growth and obtain rich seleno culture medium, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, vacuum lyophilization, obtains yeast rich in selenium.
Preferably, the preparation method of yeast rich in selenium described above, wherein said selenium ore is yeath selenium ore, and described acid solution is sulfurous acid solution, and described phosphate solution is sodium radio-phosphate,P-32 solution.
Further preferably, the preparation method of yeast rich in selenium described above specifically comprises the steps:
(1) choose even particle size, the yeath selenium ore of diameter between 40-100 order, being placed in concentration is 10%-20%(v/v) sulfurous acid solution soaks 24-48h, uses ultrasonic vibration simultaneously, and the selenium element accelerated in selenium ore dissolves;
(2) get sodium radio-phosphate,P-32 solution and carry out precipitation process to heavy metal in selenium ore, filter, filtrate is with lye pH adjustment=5.5-6.5;
(3) add the necessary Carbon and nitrogen sources of Yeast Growth and be mixed with substratum, sterilizing, inoculation yeast bacterium is cultivated, and collect yeast thalline, vacuum lyophilization is less than 15% to moisture, obtains yeast rich in selenium.
Further preferably, the preparation method of yeast rich in selenium described above, wherein the alkali lye described in step (2) is the aqueous solution of any one or more alkali following: sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, salt of wormwood, saleratus, calcium hydroxide.
Compared with prior art, the yeast rich in selenium preparation method tool that the present invention relates to has the following advantages and marked improvement:
(1) adopt the metastable selenium ore of composition as the source of selenium element, widened the selenium source material for the rich selenium of yeast, be conducive to making full use of of selenium ore resources.
(2) adopt selenium ore as the source of selenium element, avoid the use of inorganic selenite, thus improve the security of production process and yeast product.
(3) adopt phosphoric acid salt as the sinking agent of removal heavy metal element, more cheap than protein, than hydrogen sulfide safety, conveniently.
(4) organoselenium transformation efficiency is high, and the selenium content of gained yeast rich in selenium is 180-260 μ g/Kg.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done to describing further, but protection scope of the present invention be not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
The preparation of embodiment 1 yeast rich in selenium
(1) choose even particle size, the yeath selenium ore 100g of diameter between 40-100 order, be placed in 300mL sulfurous acid solution (concentration is 15%, v/v) and soak 24h, use ultrasonic vibration simultaneously, accelerate selenium ore and dissolve;
(2) get 150mL sodium radio-phosphate,P-32 solution (concentration is 10%, w/v) and precipitation process is carried out to the heavy metal in selenium ore, filter;
(3) NaOH solution of filtrate 0.1mol/L is adjusted pH to 6.0;
(4) add 10g yeast powder, 20g peptone, 20g glucose is mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL;
(5) substratum prepared is carried out sterilizing 121 DEG C, 20min;
(6) take out the nutrient solution that sterilizing completes, in nutrient solution, selenium concentration is 20 μ g/ml.The yeast (derive from vinegar unstrained spirits, residue of soya, pickles water sample respectively, 3 generations more than are cultivated in continuous passage, after testing, not containing selenium element in its thalline) of being originated by previously prepd three kinds is inoculated in nutrient solution respectively, puts 28 DEG C, 179rpm shaking table cultivation 48h;
(7) by the centrifugal 5min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) pulverized by dried yeast rich in selenium, pack with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is respectively 224 μ g/Kg, 228 μ g/Kg, 234 μ g/Kg.
The preparation of embodiment 2 yeast rich in selenium
(1) choose even particle size, the yeath selenium ore 150g of diameter between 40-100 order, be placed in the sulfurous acid solution (concentration is 20%, v/v) of 350mL and soak 48h, use ultrasonic vibration simultaneously, accelerate selenium ore and dissolve;
(2) sodium radio-phosphate,P-32 solution (concentration is 15%, w/v) getting 200mL carries out precipitation process to the heavy metal in selenium ore, filters;
(3) by the Na of filtrate 0.1mol/L 2cO 3solution adjusts pH to 6.0;
(4) add 10g analysis for soybean powder, 20g peptone, 20g sucrose is mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL;
(5) substratum prepared is carried out sterilizing 121 DEG C, 20min;
(6) nutrient solution that sterilizing completes is taken out, in nutrient solution, selenium concentration is 18 μ g/ml, the yeast that previously prepd three kinds is originated (is derived from vinegar unstrained spirits, residue of soya, pickles water sample respectively, in 3 generations more than, are cultivated in continuous passage, after testing, not containing selenium element in its thalline) be inoculated into respectively in nutrient solution, put 28 DEG C, 179rpm shaking table cultivation 60h;
(7) by the centrifugal 8min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) pulverized by dried yeast rich in selenium, pack with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is 198 μ g/Kg, 205 μ g/Kg, 214 μ g/Kg.
The preparation of embodiment 3 yeast rich in selenium
(1) choose even particle size, the yeath selenium ore 200g of diameter between 40-100 order, be placed in the sulfurous acid solution (concentration is 20%, v/v) of 400mL and soak 48h.Use ultrasonic vibration simultaneously, accelerate selenium ore and dissolve;
(2) sodium radio-phosphate,P-32 solution (concentration is 10%, w/v) getting 200mL carries out precipitation process to the heavy metal in selenium ore;
(3) KOH solution of filtrate 0.1mol/L is adjusted pH to 6.0;
(4) add 10g wheat bran juice, 20g peptone, 20g Semen Maydis powder be mixed with liquid nutrient medium, be sub-packed in Erlenmeyer flask, loading amount 50mL/250mL.;
(5) substratum prepared is carried out sterilizing 121 DEG C, 20min;
(6) nutrient solution that sterilizing completes is taken out, in nutrient solution, selenium concentration is 22 μ g/ml, three saccharomycetes that previously prepd three kinds is originated (are derived from vinegar unstrained spirits, residue of soya, pickles water sample respectively, in 3 generations more than, are cultivated in continuous passage, after testing, not containing selenium element in its thalline) be inoculated into respectively in nutrient solution, put 28 DEG C, 179rpm shaking table cultivation 72h;
(7) by the centrifugal 8min of bacterium liquid 3000r/min, collect yeast thalline, vacuum lyophilization, moisture is less than 15%;
(8) pulverized by dried yeast rich in selenium, pack with air-locked packing bag, can obtain the yeast that selenium content is high, the selenium content of dry yeast is 235 μ g/Kg, 243 μ g/Kg, 228 μ g/Kg.
Comparative example 1
Adopt granite porphyry selenium ore as the source of selenium element, yeast nutrient solution is obtained through processing mode in the same manner as in Example 1, carry out under identical condition cultivating, centrifugal, dry, pulverize, packaging, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 105 μ g/Kg, 118 μ g/Kg, 98 μ g/Kg.
Comparative example 2
Adopt carbonatite selenium ore as the source of selenium element, yeast nutrient solution is obtained through processing mode in the same manner as in Example 1, carry out under identical condition cultivating, centrifugal, dry, pulverize, packaging, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 110 μ g/Kg, 102 μ g/Kg, 106 μ g/Kg.
Comparative example 3
Adopt skarn selenium ore as the source of selenium element, yeast nutrient solution is obtained through processing mode in the same manner as in Example 1, carry out under identical condition cultivating, centrifugal, dry, pulverize, packaging, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 90 μ g/Kg, 96 μ g/Kg, 85 μ g/Kg.
Comparative example 4
Adopt carbon siliceous slate selenium ore as the source of selenium element, yeast nutrient solution is obtained through processing mode in the same manner as in Example 1, carry out under identical condition cultivating, centrifugal, dry, pulverize, packaging, obtain equally containing selenium yeast bacterium, three saccharomycete dry yeast selenium contents are respectively 79 μ g/Kg, 104 μ g/Kg, 93 μ g/Kg.

Claims (3)

1. the preparation method of a yeast rich in selenium, it is characterized in that the method comprises the steps: selenium ore by acid soak dissolved selenium element, phosphate solution settlement treatment heavy metal, filters, and regulates filtrate pH=5.5-6.5, add the necessary Carbon and nitrogen sources of Yeast Growth and obtain rich seleno culture medium, sterilizing, inoculation yeast bacterium is cultivated, and collects yeast thalline, vacuum lyophilization, obtains yeast rich in selenium; Described selenium ore is yeath selenium ore, and described acid solution is sulfurous acid solution, and described phosphate solution is sodium radio-phosphate,P-32 solution.
2. the preparation method of yeast rich in selenium according to claim 1, is characterized in that the method specifically comprises the steps:
(1) choose even particle size, the yeath selenium ore of diameter between 40-100 order, being placed in concentration is that 10%-20% sulfurous acid solution soaks 24-48h, uses ultrasonic vibration simultaneously, and the selenium element accelerated in selenium ore dissolves;
(2) get sodium radio-phosphate,P-32 solution and carry out precipitation process to heavy metal in selenium ore, filter, filtrate is with lye pH adjustment=5.5-6.5;
(3) add the necessary Carbon and nitrogen sources of Yeast Growth and be mixed with substratum, sterilizing, inoculation yeast bacterium is cultivated, and collect yeast thalline, vacuum lyophilization is less than 15% to moisture, obtains yeast rich in selenium.
3. the preparation method of yeast rich in selenium according to claim 2, is characterized in that: the alkali lye described in step (2) is the aqueous solution of any one or more alkali following: sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, salt of wormwood, saleratus, calcium hydroxide.
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CN104480031A (en) * 2014-12-26 2015-04-01 山东圣琪生物有限公司 Iodine-enriched yeast preparation method and iodine-enriched yeast prepared according to method
CN105331546B (en) * 2015-11-23 2018-09-25 湖北工业大学 A kind of selenium-rich Candida glabrata strain FXY-4 and its cultural method and the application as fish feed additive
CN107011030A (en) * 2017-04-06 2017-08-04 安徽康坶国际化肥股份有限公司 A kind of Rice enriched-Se paddy rice organic selenium fertilizer and preparation method
CN107173411A (en) * 2017-07-10 2017-09-19 李郑松 A kind of preparation method of nutrition Selenium-enriched fermentation biscuit
CN107594070A (en) * 2017-09-08 2018-01-19 四川亿客农食品有限公司 A kind of breeding method of Se-rich farm products and its application
CN107663123A (en) * 2017-10-11 2018-02-06 广西南宁秀珀生物科技有限公司 Se-enriched bio fertilizer and preparation method thereof
CN107698288A (en) * 2017-10-11 2018-02-16 广西南宁秀珀生物科技有限公司 Bio-fertilizer production system with duplicate removal metal function
CN107586167A (en) * 2017-10-11 2018-01-16 广西南宁秀珀生物科技有限公司 Biological Selenium fertilizer manufacture system with duplicate removal metal function
CN107500843A (en) * 2017-10-11 2017-12-22 广西南宁秀珀生物科技有限公司 bio-fertilizer production system
CN107445674A (en) * 2017-10-11 2017-12-08 广西南宁秀珀生物科技有限公司 Se-enriched bio fertilizer production system
CN107652040A (en) * 2017-10-11 2018-02-02 广西南宁秀珀生物科技有限公司 Bio-fertilizer and preparation method thereof

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CN1888048A (en) * 2006-07-21 2007-01-03 江南大学 Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source
CN101402976A (en) * 2008-10-22 2009-04-08 四川正圆科技有限公司 Method for producing mineral composite yeast selenium by employing selenium-containing coal gangue
CN102703561A (en) * 2012-05-26 2012-10-03 彭祚全 Method for producing activated selenium mineral powder by microbial fermentation

Patent Citations (3)

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CN1888048A (en) * 2006-07-21 2007-01-03 江南大学 Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source
CN101402976A (en) * 2008-10-22 2009-04-08 四川正圆科技有限公司 Method for producing mineral composite yeast selenium by employing selenium-containing coal gangue
CN102703561A (en) * 2012-05-26 2012-10-03 彭祚全 Method for producing activated selenium mineral powder by microbial fermentation

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