CN103865816A - Yeast powder rich in selenium and germanium - Google Patents
Yeast powder rich in selenium and germanium Download PDFInfo
- Publication number
- CN103865816A CN103865816A CN201210542956.5A CN201210542956A CN103865816A CN 103865816 A CN103865816 A CN 103865816A CN 201210542956 A CN201210542956 A CN 201210542956A CN 103865816 A CN103865816 A CN 103865816A
- Authority
- CN
- China
- Prior art keywords
- yeast
- germanium
- selenium
- powder
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a culture medium formula and a fermentation process for a yeast powder rich in selenium and germanium. According to the invention, in virtue of the enrichment effect of microzyme, a proper amount of germanium powder and a selenium compound are added in the process of microzyme culture and yeast powder rich in selenium and germanium is obtained through biological growth and decomposition. The yeast powder is rich in trace elements like selenium and germanium which are beneficial to a human body and is a good base material for other functional food reinforcing trace elements.
Description
Technical field
The culture medium prescription and the zymotechnique that the invention provides a kind of rich selenium, germanium yeast powder, belong to food fermentation field.
Background technology
Selenium is respectfully called " kindling material of life " by domestic and international the world of medicine and nutrition educational circles, enjoys the good reputation such as " long-lived element ", " anticancer king ", " heart patron saint " " natural toxinicide ".Selenium is 1/10000000th at tissue intensive amount, but it has determined the existence of life, is that other materials are irreplaceable to the great function of human health.Scarce selenium can directly cause human immunological competence to decline, clinical medicine proves, threaten 40 various diseases of human health and life all relevant with the scarce selenium of human body, as cancer, cardiovascular diseases, hepatopathy, cataract, pancreas disease, diabetes, reproductive system disease etc.According to expert's textual criticism, people needs to mend throughout one's life selenium.No matter be experimentation on animals or clinical practice, the selenium that should constantly obtain q.s from diet has all been described, can not supplement in time, will reduce the ability of getting rid of illness.
When organic germanium compounds runs into tumour cell, its normal center can increase the energy of position of tumour cell, reduces its mobility, thereby plays the effect that suppresses and kill tumour cell.Except antitumor and immune actification, germanium is of value to the Function that biological effect also comprises hemopoietic system, and cell growth inhibiting promotes antibacterial disappearance, the effects such as Promoting plant growth.The effect of blood system is mainly manifested in to the increase that stimulates red corpuscle and amount of hemoglobin in blood, treatment anaemia is had certain effect.
Can cause poisoningly but the germanium of occurring in nature is directly edible, after only having and absorbing by organism, inorganic Ge can be converted into organic germanium, could be utilized by human body, thereby play multiple beneficial effect.Yeast rich in selenium powder has entered suitability for industrialized production, but germanium-enriched yeast powder also do not occur, is rich in selenium, two kinds of micro-yeast powders of germanium blank especially.
summary of the invention:
The invention provides culture medium prescription and the zymotechnique of a kind of rich selenium, germanium yeast powder, by long-term scientific research test, find that wine yeast and saccharomyces cerevisiae show well in following culture medium prescription, can either reach the suitable amount containing selenium and organic germanium, there is again higher output, be easy to industrial production and apply, this formula is: the yeast extract that adds 0.4-1.0% in the wort of 10-13 degree, add again 10% Sodium Selenite sterile water solution, make the concentration of selenium at 4-8ppm; Add again germanite powder, make the concentration of germanium at 80-120ppm, regulate pH value to 5.9-6.1.
Technical scheme of the present invention:
Inoculation yeast bacterial classification in test-tube culture medium, at 29-31 DEG C, first leaves standstill and cultivates 14-17 hour, and then shaking culture 14-17 hour; Then in 1:(800-1000) ratio, culture transferring is in fermention medium, the formula of fermention medium is: in the wort of 10-13 degree, add the yeast extract of 0.4-1.0%, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 4-8ppm; Add again germanite powder, make the concentration of germanium at 80-120ppm, regulate pH value to 5.9-6.1.Postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 29-31 DEG C of temperature, air flow 1.4-1.7vvm, cultivate 20-30 hour, finish fermentation; By centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 55-65 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
The yeast dry powder obtaining by above technical scheme, the content of selenium can reach 400-550ppm, and the content of germanium can reach 700-900ppm.
The technical problem that the present invention solves:
The yeast powder of making by technique of the present invention, the inorganic Ge that cannot be utilized by human body is converted into the organic germanium that can be utilized by human body; And this yeast powder contains selenium and two kinds of trace elements of organic germanium of appropriate be absorbed by the body utilization simultaneously, strengthen these two kinds of micro-functional foodstuffs a kind of easy to use, inexpensive base-material is provided for making other, contain the selenium of appropriate amount and two kinds of trace elements of organic germanium simultaneously and also filled up the blank of this series products.
Embodiment
Embodiment 1:
1, in test-tube culture medium, inoculate wine yeast bacterial classification, at 29 DEG C, first standing cultivation 14 hours, and then shaking culture 14 hours;
2, then in the ratio of 1:800, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 10 degree, add 0.4% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 4ppm; Add again germanite powder, make the concentration of germanium at 80ppm, regulate pH value to 5.9.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 29 DEG C of temperature, air flow 1.4vvm, cultivate 20 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 55 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 428ppm through measuring containing the amount of selenium, germanic 731ppm.
Embodiment 2:
1, in test-tube culture medium, inoculate wine yeast bacterial classification, at 31 DEG C, first standing cultivation 17 hours, and then shaking culture 17 hours;
2, then in the ratio of 1:1000, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 13 degree, add 1.0% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 8ppm; Add again germanite powder, make the concentration of germanium at 120ppm, regulate pH value to 6.1.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 31 DEG C of temperature, air flow 1.7vvm, cultivate 30 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 65 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 443ppm through measuring containing the amount of selenium, germanic 715ppm.
Embodiment 3:
1, in test-tube culture medium, inoculate wine yeast bacterial classification, at 30 DEG C, first standing cultivation 16 hours, and then shaking culture 16 hours;
2, then in the ratio of 1:900, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 12 degree, add 0.7% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 6ppm; Add again germanite powder, make the concentration of germanium at 100ppm, regulate pH value to 6.0.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 30 DEG C of temperature, air flow 1.6vvm, cultivate 25 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 60 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 538ppm through measuring containing the amount of selenium, germanic 896ppm.
Embodiment 4:
1, in test-tube culture medium, inoculate cereuisiae fermentum bacterial classification, at 29 DEG C, first standing cultivation 14 hours, and then shaking culture 14 hours;
2, then in the ratio of 1:800, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 10 degree, add 0.4% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 4ppm; Add again germanite powder, make the concentration of germanium at 80ppm, regulate pH value to 5.9.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 29 DEG C of temperature, air flow 1.4vvm, cultivate 20 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 55 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 451ppm through measuring containing the amount of selenium, germanic 767ppm.
Embodiment 5:
1, in test-tube culture medium, inoculate cereuisiae fermentum bacterial classification, at 31 DEG C, first standing cultivation 17 hours, and then shaking culture 17 hours;
2, then in the ratio of 1:1000, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 13 degree, add 1.0% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 8ppm; Add again germanite powder, make the concentration of germanium at 120ppm, regulate pH value to 6.1.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 31 DEG C of temperature, air flow 1.7vvm, cultivate 30 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 65 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 461ppm through measuring containing the amount of selenium, germanic 755ppm.
Embodiment 6:
1, in test-tube culture medium, inoculate wine yeast bacterial classification, at 30 DEG C, first standing cultivation 15 hours, and then shaking culture 15 hours;
2, then in the ratio of 1:900, culture transferring is in fermention medium.The formula of fermention medium is: in the wort of 11 degree, add 0.8% yeast extract, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 7ppm; Add again germanite powder, make the concentration of germanium at 100ppm, regulate pH value to 6.0.
3, postvaccinal fermention medium is cultivated in fermentor tank, kept the fermentation condition of 30 DEG C of temperature, air flow 1.5vvm, cultivate 25 hours, finish fermentation;
4, by centrifugation yeast, then repeatedly rinse yeast by purified water, the cleaner yeast of centrifugal acquisition; At 60 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
Gained yeast dry powder is 542ppm through measuring containing the amount of selenium, germanic 887ppm.
Claims (3)
1. rich selenium, a germanium yeast powder, is characterized in that, is: add the yeast extract of 0.4-1.0% at the wort of 10-13 degree, then add 10% Sodium Selenite sterile water solution, make the concentration of selenium at 4-8ppm for the formula of the substratum of saccharomycetes to make fermentation; Add again germanite powder, make the concentration of germanium at 80-120ppm, regulate pH value to 5.9-6.1.
2. the rich selenium of one as claimed in claim 1, germanium yeast powder, it is characterized in that the following culture process of described yeast process: inoculation yeast bacterial classification in test-tube culture medium, at 29-31 DEG C, first leave standstill and cultivate 14-17 hour, and then shaking culture 14-17 hour; Then in 1:(800-1000) ratio, culture transferring to as claimed in claim 1 formula substratum in; In fermentor tank, cultivate, keep the fermentation condition of 29-31 DEG C of temperature, air flow 1.4-1.7vvm, cultivate 20-30 hour, finish fermentation; By centrifugation yeast, then repeatedly rinse yeast by purified water, the purer yeast thalline of centrifugal acquisition; At 55-65 DEG C, be dried to moisture content below 3%, after pulverizing, be finished product.
3. the rich selenium of one as claimed in claim 2, germanium yeast powder, is characterized in that, described barms is cereuisiae fermentum bacterial classification and wine yeast bacterial classification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210542956.5A CN103865816A (en) | 2012-12-15 | 2012-12-15 | Yeast powder rich in selenium and germanium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210542956.5A CN103865816A (en) | 2012-12-15 | 2012-12-15 | Yeast powder rich in selenium and germanium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103865816A true CN103865816A (en) | 2014-06-18 |
Family
ID=50904828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210542956.5A Pending CN103865816A (en) | 2012-12-15 | 2012-12-15 | Yeast powder rich in selenium and germanium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103865816A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723322A (en) * | 2017-11-28 | 2018-02-23 | 陕西省微生物研究所 | A kind of preparation method of amillariella mellea selenium germanium polysaccharide |
| CN109247515A (en) * | 2018-08-10 | 2019-01-22 | 安徽硒无忧现代农业科技有限公司 | A kind of preparation process of richness germanium malt flour |
| CN109355213A (en) * | 2018-08-24 | 2019-02-19 | 珲春市龙裕农业发展集团有限公司 | The preparation method of the double rich beef cattle bio-additives of selenium germanium |
| CN109717476A (en) * | 2019-02-25 | 2019-05-07 | 江苏诺普乐生物科技有限公司 | A kind of high calcium yeast powder and a kind of high calcium yeast powder, preparation method thereof |
-
2012
- 2012-12-15 CN CN201210542956.5A patent/CN103865816A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723322A (en) * | 2017-11-28 | 2018-02-23 | 陕西省微生物研究所 | A kind of preparation method of amillariella mellea selenium germanium polysaccharide |
| CN107723322B (en) * | 2017-11-28 | 2021-03-02 | 陕西省微生物研究所 | Preparation method of armillaria selenogermani polysaccharide |
| CN109247515A (en) * | 2018-08-10 | 2019-01-22 | 安徽硒无忧现代农业科技有限公司 | A kind of preparation process of richness germanium malt flour |
| CN109355213A (en) * | 2018-08-24 | 2019-02-19 | 珲春市龙裕农业发展集团有限公司 | The preparation method of the double rich beef cattle bio-additives of selenium germanium |
| CN109717476A (en) * | 2019-02-25 | 2019-05-07 | 江苏诺普乐生物科技有限公司 | A kind of high calcium yeast powder and a kind of high calcium yeast powder, preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102242044B (en) | Cordyceps cicadae wine and preparation method thereof | |
| CN103299887B (en) | Method for preparing phellinus igniarius hypha blocks by utilizing sprouted rice | |
| CN100362091C (en) | A selenium yeast product, a method of preparing a selenium yeast product and the use of the product for preparing food, a dietary supplement or a drug | |
| CN102552335B (en) | Traditional Chinese medicine health care product, its preparation method and its application | |
| CN107960557A (en) | The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed | |
| CN108771242A (en) | A kind of coronoid process dissipate capsule bacterium Fermentation Function compound and its preparation method and application | |
| CN101864369A (en) | Yeast with strong capacities of tolerating, enriching and converting organic selenium and application thereof | |
| CN104711203A (en) | Selenium-rich germanium yeast powder | |
| CN105027970A (en) | Pleurotus tuber-regium culture medium rich in selenium and germanium and preparing method thereof | |
| CN107593955A (en) | Ferment edible mushroom tea preparation process and fermentation edible mushroom tea | |
| CN106148200B (en) | culture medium of selenium-rich low-lead and arsenic cordyceps sinensis mycelia and cultivation method of culture medium | |
| CN101508961A (en) | Method of preparing yeast rich in copper | |
| CN112094753A (en) | Nutrient solution for culturing ganoderma lucidum and method for culturing ganoderma lucidum by fermentation process | |
| CN107114758A (en) | A kind of ferment cordyceps sinensis D-mannitol powder and preparation method thereof | |
| CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN110257296B (en) | Culture method and application of chromium-enriched spirulina | |
| CN103865816A (en) | Yeast powder rich in selenium and germanium | |
| CN103948023A (en) | Health foods for enhancing immunity and improving sleep and two-step fermentation preparation method thereof | |
| CN102475326A (en) | Oral liquid of microecological mulberry fruits | |
| CN109220536A (en) | A kind of inoculation method of organic selenium-rich Cordyceps militaris | |
| CN103109676A (en) | Culture method for zinc-and-selenium-rich edible fungus | |
| CN108175040A (en) | A kind of preparation method of millet oat Antrodia camphorata mycoplasma health food | |
| CN105145115B (en) | A kind of production method of organic Se-rich zinc-rich product | |
| CN107027516A (en) | A kind of selenium-rich Periostracum cicadae, its cultural method and application | |
| CN103704023A (en) | Method for cultivating functional cordyceps militaris |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140618 |