CN107960557A - The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed - Google Patents
The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed Download PDFInfo
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- CN107960557A CN107960557A CN201711427506.0A CN201711427506A CN107960557A CN 107960557 A CN107960557 A CN 107960557A CN 201711427506 A CN201711427506 A CN 201711427506A CN 107960557 A CN107960557 A CN 107960557A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses the method that a primary yeast fermenting and producing prepares selenium-rich chicken feed, yeast-inoculated is tamed in the nutrient solution of addition sodium selenite in the present invention, filter out Se-enriched yeast strain, fermentation substrate is used as by primary raw material of selenium-rich peanuts shell, stalk and selenium-rich corn, carry out the fermenting and producing of selenium-enriched protein feed, the inorganic selenium in feedstuff is promoted to be converted into Organic Selenium by the physiological action of strain, beneficial to absorption of the chicken body to selenium, so as to promote enrichment of the selenium in chicken body, animal immunizing power is improved, increases economic benefit；Handled at the same time through aerobic anaerobic fermentation, improve the efficiency of fermentation, add feed palatability, ensure that the chicken body absorptivity higher to yeast selenium feed, supplement improves daily feed nutrition.
Description
Technical field
The present invention relates to fermented feed technical field, and in particular to a primary yeast fermenting and producing prepares the side of selenium-rich chicken feed
Method.
Background technology
In recent years, selenium becomes the multi-disciplinary research hotspots such as micro- science, nutrition, immunology.Selenium has anti-
Oxidation, slow down aging, the effect such as the accumulation of heavy metal, releasing heavy metal poisoning in suppression tumour, removing machine body, and selenium is still
The natural antidote of part heavy metal element.At present, the replenishers as selenium, inorganic sodium selenite are still main addition shape
Formula, sodium selenite absorptivity is higher, is generally not present utilization rate problem, and still, sodium selenite toxicity is stronger, along with its absorption
Rate is high, and addition is improper to often result in animal poisoning.Compared with inorganic selenium, Organic Selenium is with absorptivity is high, bioactivity is strong, toxicity
Small, the characteristics of environmental pollution is small, become an emphasis of current selenium research.Advocate in the world using Organic Selenium replace inorganic selenium as
The nutritional supplement of selenium, and developed many products for being rich in Organic Selenium.
Inorganic selenium can be changed into Organic Selenium by selenium-enriched probiotics by probiotics, according to surveying and determination Organic Selenium in selenium-enriched probiotics
Content is more than 90%, and for selenomethionine content more than 75%, prebiotic bacterium number is more than 1010CFU/ml.Selenium-enriched probiotics can promote Asia
Sodium selenate is converted to Organic Selenium, and Organic Selenium has higher absorption rate, so that it is heavy in body tissue to add selenium
Accumulated amount, plays the double action of Organic Selenium and probiotics, significantly improves the production performance of animal, strengthen animal immune function and
Premunition.
The content of the invention
The present invention provides the method that a primary yeast fermenting and producing prepares selenium-rich chicken feed, Se content in feed is improved, is increased
Add economic benefit.
The present invention uses following technical scheme：
The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed, including step in detail below：
（1）Prepare solid fermentation substrate：
60-75 parts of selenium-rich peanuts shells, 42-60 parts of selenium-rich stalks, 52-86 parts of selenium-rich corns after crushing are taken to be placed in mixer, first
Mixing 3-8 minutes, then 10-18 parts of molasses and suitable quantity of water are added, and 0.3-2 parts of sodium selenites are added, adjust pH value with limewash
To 4.8-5.5, mix 5-10 minutes again, after gained mixture is carried out moist heat sterilization, obtain solid fermentation substrate, it is spare；
（2）Saccharomycete domestication culture：
Yeast strain after activation is seeded in Na by 10% inoculum concentration2SeO3Concentration is followed successively by 10,15,20,25,30,35,40
In the basal medium of μ g/ml, selenium concentration gradient domestication is carried out at 28-30 DEG C, per the domestication time in generation is 36-48h, then
Se content in saccharomycete is measured, so that the strong yeast seeds of selenium rich ability are obtained, it is spare；
（3）Strain expands culture：
Culture is amplified to selenium-rich Saccharomyces cerevisiae bacterium strain, the aerobic fermentation 18- under the conditions of 28-30 DEG C, humidity 60-70%
20h, obtains yeast liquid fermentation culture；
Liquid anaerobic fermentation is carried out afterwards, and fermentation time is in 26-34h；
（4）Solid fermentation：
The step of by half（3）Anaerobic liquid zymotic fluid and step（1）Solid fermentation substrate mixes, and is then issued in aerobic situation
Ferment 3-5h, then pack, when often filling 30-40cm thickness remaining liquid fermentation liquid, sealing hair are uniformly sprayed in solid fermentation substrate surface
Ferment 5-8 days；
（5）Use low temperature drying to mixture water content below 8% the mixture after above-mentioned solid fermentation, add 0.2-0.5
Part salt, 2-4 parts of feed addictives, mix, to obtain the final product.
Wherein, the yeast strain uses Candida bacteria strain or saccharomyces cerevisiae bacteria strain.
Wherein, the step（2）Saccharomycete tames condition of culture in 32-35 DEG C of temperature, the constant temperature incubation of 50-60r/min
Culture in case.
Wherein, through step（3）After strain expands culture, viable count reaches 0.8-1.0 × 109 CFU/mL。
Wherein, the step（5）In solid fermentation, total anaerobic liquid zymotic fluid is with solid fermentation substrate according to 1:0.8-3
Weight ratio mixing.
The present invention has the beneficial effect that：
Yeast-inoculated is tamed in the nutrient solution of addition sodium selenite in the present invention, filters out Se-enriched yeast strain, with
Selenium-rich peanuts shell, stalk and selenium-rich corn for primary raw material be used as fermentation substrate, progress selenium-enriched protein feed fermenting and producing, make
Selenium-rich raw material comes into full contact with bacterium solution, promotes the inorganic selenium in feedstuff to be converted into Organic Selenium by the physiological action of strain, profit
In absorption of the chicken body to selenium, so as to promote enrichment of the selenium in chicken body, the preventive and therapeutic effect of Animal diseases can be strengthened, improve animal
Immunity, increases economic benefit；At the same time through aerobic-anaerobic fermentation processing, the efficiency of fermentation is improved, it is agreeable to the taste to add feed
Property, it ensure that the chicken body absorptivity higher to yeast selenium feed, supplement improves daily feed nutrition.
Embodiment
The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed, including step in detail below：
（1）Prepare solid fermentation substrate：
Take 65 parts of selenium-rich peanuts shells, 45 parts of selenium-rich stalks, 75 parts of selenium-rich corns after crushing to be placed in mixer, first mix 5 minutes,
12 parts of molasses and suitable quantity of water are added again, and adds 0.6 part of sodium selenite, are adjusted pH value to 5.0 with limewash, are mixed 8 points again
Clock, after gained mixture is carried out moist heat sterilization, obtains solid fermentation substrate, spare；
（2）Saccharomycete domestication culture：
Candida bacterial strain after activation is seeded in Na by 10% inoculum concentration2SeO3Concentration is followed successively by 10,15,20,25,30,
35th, in the basal medium of 40 μ g/ml, selenium concentration gradient domestication is carried out at 28-30 DEG C, per the domestication time in generation is 45h, so
Se content in saccharomycete is measured afterwards, so that the strong yeast seeds of selenium rich ability are obtained, it is spare；
（3）Strain expands culture：
Culture is amplified to selenium-rich Saccharomyces cerevisiae bacterium strain, the aerobic fermentation 20h under the conditions of 30 DEG C, humidity 65%, obtains yeast
Liquid fermentation and culture liquid；
Liquid anaerobic fermentation is carried out afterwards, and fermentation time is in 30h；
（4）Solid fermentation：
The step of by half（3）Anaerobic liquid zymotic fluid and step（1）Solid fermentation substrate mixes, and is then issued in aerobic situation
Ferment 4h, then pack, remaining liquid fermentation liquid is uniformly sprayed in solid fermentation substrate surface when often filling 35cm thickness, is sealed by fermentation 7 days；
（5）Use low temperature drying to mixture water content below 8% the mixture after above-mentioned solid fermentation, add 0.3 portion of food
Salt, 3 parts of feed addictives, mix, to obtain the final product.
Wherein, the step（2）Saccharomycete tame condition of culture 34 DEG C of temperature, 55r/min constant incubator in train
Support.
Wherein, through step（3）After strain expands culture, viable count reaches 1.0 × 109 CFU/mL。
Wherein, the step（5）In solid fermentation, total anaerobic liquid zymotic fluid is with solid fermentation substrate according to 1:3 weight
Amount is than mixing.
Claims (5)
1. the method that a primary yeast fermenting and producing prepares selenium-rich chicken feed, it is characterised in that including step in detail below：
（1）Prepare solid fermentation substrate：
60-75 parts of selenium-rich peanuts shells, 42-60 parts of selenium-rich stalks, 52-86 parts of selenium-rich corns after crushing are taken to be placed in mixer, first
Mixing 3-8 minutes, then 10-18 parts of molasses and suitable quantity of water are added, and 0.3-2 parts of sodium selenites are added, adjust pH value with limewash
To 4.8-5.5, mix 5-10 minutes again, after gained mixture is carried out moist heat sterilization, obtain solid fermentation substrate, it is spare；
（2）Saccharomycete domestication culture：
Yeast strain after activation is seeded in Na by 10% inoculum concentration2SeO3Concentration is followed successively by 10,15,20,25,30,35,40 μ
In the basal medium of g/ml, selenium concentration gradient domestication is carried out at 28-30 DEG C, per the domestication time in generation is 36-48h, is then surveyed
Determine Se content in saccharomycete, so that the strong yeast seeds of selenium rich ability are obtained, it is spare；
（3）Strain expands culture：
Culture is amplified to selenium-rich Saccharomyces cerevisiae bacterium strain, the aerobic fermentation 18- under the conditions of 28-30 DEG C, humidity 60-70%
20h, obtains yeast liquid fermentation culture；
Liquid anaerobic fermentation is carried out afterwards, and fermentation time is in 26-34h；
（4）Solid fermentation：
The step of by half（3）Anaerobic liquid zymotic fluid and step（1）Solid fermentation substrate mixes, and is then issued in aerobic situation
Ferment 3-5h, then pack, when often filling 30-40cm thickness remaining liquid fermentation liquid, sealing hair are uniformly sprayed in solid fermentation substrate surface
Ferment 5-8 days；
（5）Use low temperature drying to mixture water content below 8% the mixture after above-mentioned solid fermentation, add 0.2-0.5
Part salt, 2-4 parts of feed addictives, mix, to obtain the final product.
2. the method that primary yeast fermenting and producing according to claim 1 prepares selenium-rich chicken feed, it is characterised in that described
Yeast strain uses Candida bacteria strain or saccharomyces cerevisiae bacteria strain.
3. the method that primary yeast fermenting and producing according to claim 1 prepares selenium-rich chicken feed, it is characterised in that described
Step（2）Saccharomycete tame condition of culture 32-35 DEG C of temperature, 50-60r/min constant incubator in cultivate.
4. the method that primary yeast fermenting and producing according to claim 1 prepares selenium-rich chicken feed, it is characterised in that through step
Suddenly（3）After strain expands culture, viable count reaches 0.8-1.0 × 109 CFU/mL。
5. the method that primary yeast fermenting and producing according to claim 1 prepares selenium-rich chicken feed, it is characterised in that described
Step（5）In solid fermentation, total anaerobic liquid zymotic fluid is with solid fermentation substrate according to 1:The weight ratio mixing of 0.8-3.
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| CN201711427506.0A CN107960557A (en) | 2017-12-26 | 2017-12-26 | The method that one primary yeast fermenting and producing prepares selenium-rich chicken feed |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109232107A (en) * | 2018-11-19 | 2019-01-18 | 黑龙江牧康牧业有限责任公司 | Yeast selenium foliar fertilizer, production method and application |
| CN110150459A (en) * | 2019-06-11 | 2019-08-23 | 四川省粮油科研所 | A kind of livestock and poultry gastric content Selenium-enriched fermentation raw material and its preparation method and application |
| CN111000037A (en) * | 2020-02-20 | 2020-04-14 | 暮羊羊生物科技有限公司 | High-selenium auxiliary anti-cancer goat milk and production method thereof |
| CN111631302A (en) * | 2020-05-20 | 2020-09-08 | 百色三宝生物技术有限公司 | Method for producing organic selenium by solid two-stage fermentation |
| CN112450326A (en) * | 2020-09-09 | 2021-03-09 | 南宁学院 | Chicken feed containing dietary fiber and probiotics and preparation method thereof |
| CN113693157A (en) * | 2021-08-30 | 2021-11-26 | 焦作市佰役安生物工程有限公司 | Preparation method of selenium-rich feed additive |
| CN114794303A (en) * | 2022-05-10 | 2022-07-29 | 吉林省农业科学院 | Selenium-rich rhodotorula mucilaginosa, selenium-rich rhodotorula mucilaginosa feed additive and preparation method and application thereof |
| CN114854611A (en) * | 2022-02-16 | 2022-08-05 | 中国农业科学院油料作物研究所 | Selenium-enriched yeast and probiotic compound bacterium liquid and fermented soybean meal feed |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109232107A (en) * | 2018-11-19 | 2019-01-18 | 黑龙江牧康牧业有限责任公司 | Yeast selenium foliar fertilizer, production method and application |
| CN110150459A (en) * | 2019-06-11 | 2019-08-23 | 四川省粮油科研所 | A kind of livestock and poultry gastric content Selenium-enriched fermentation raw material and its preparation method and application |
| CN111000037A (en) * | 2020-02-20 | 2020-04-14 | 暮羊羊生物科技有限公司 | High-selenium auxiliary anti-cancer goat milk and production method thereof |
| CN111631302A (en) * | 2020-05-20 | 2020-09-08 | 百色三宝生物技术有限公司 | Method for producing organic selenium by solid two-stage fermentation |
| CN112450326A (en) * | 2020-09-09 | 2021-03-09 | 南宁学院 | Chicken feed containing dietary fiber and probiotics and preparation method thereof |
| CN113693157A (en) * | 2021-08-30 | 2021-11-26 | 焦作市佰役安生物工程有限公司 | Preparation method of selenium-rich feed additive |
| CN114854611A (en) * | 2022-02-16 | 2022-08-05 | 中国农业科学院油料作物研究所 | Selenium-enriched yeast and probiotic compound bacterium liquid and fermented soybean meal feed |
| CN114854611B (en) * | 2022-02-16 | 2023-12-15 | 中国农业科学院油料作物研究所 | Selenium-enriched saccharomycetes, probiotics composite bacterial liquid and fermented soybean meal feed |
| CN114794303A (en) * | 2022-05-10 | 2022-07-29 | 吉林省农业科学院 | Selenium-rich rhodotorula mucilaginosa, selenium-rich rhodotorula mucilaginosa feed additive and preparation method and application thereof |
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Application publication date: 20180427 |