CN109007805A - A kind of hawthorn probiotics ferment and preparation method thereof - Google Patents
A kind of hawthorn probiotics ferment and preparation method thereof Download PDFInfo
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- CN109007805A CN109007805A CN201810805189.XA CN201810805189A CN109007805A CN 109007805 A CN109007805 A CN 109007805A CN 201810805189 A CN201810805189 A CN 201810805189A CN 109007805 A CN109007805 A CN 109007805A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to ferment production technical fields, and in particular to a kind of hawthorn probiotics ferment and preparation method thereof.The present invention is using hawthorn, Chinese yam, Fructus Corni as primary raw material, by fermenting three times.The nutritional ingredient of raw material of the present invention is more abundant in scientific and reasonable collocation, under the fermentation of probiotics, the type, dosage of strict control probiotics and fermentation duration simultaneously, guarantee raw material fermentation more sufficiently, depth, finally enable the nutriment in raw material, active material, antioxidant to discharge to decomposite completely and, obtains the hawthorn probiotics ferment of a kind of good mouthfeel, full of nutrition, spleen benefiting and stimulating the appetite, relieving dyspepsia.
Description
Technical field
The invention belongs to ferment production technical fields, and in particular to a kind of hawthorn probiotics ferment and preparation method thereof.
Background technique
Ferment, which refers to, ferments to fruit and vegetable by microorganism, a kind of liquid containing bioactive ingredients of extraction.Above-mentioned life
Object active constituent includes enzyme but is not limited to enzyme, including active antioxidants, biomass element etc., from fermentation participate in bacterium and
Food materials for fermentation.
The ferment rationally made, the active substance of plant extracted are from food materials, and participation bacterium of fermenting is even more people
The component part of body microecosystem.Therefore, the side effect of ferment is almost nil, powerful effect, minimum side effect, in recent years
Come always in explosive increase trend.
Hawthorn stone fruit, caryoplasm is hard, and pulp is thin, and taste is slightly sour puckery, and fruit is raw-eaten or makees preserved fruit fruitcake, can enter after drying
Medicine is the distinctive dual-purpose of drug and fruit tree species of China, has the effects that reducing blood lipid, blood pressure, heart tonifying, arrhythmia, while being also strong
Spleen appetizing, relieving dyspepsia, phlegm reduction of blood circulation promoting good medicine, the diseases such as, hernia, blood stasis full to chest diaphragm spleen, amenorrhoea have good curative effect.
Haw products are more single in the market, but since the haw berry body maturation phase compares concentration, and be difficult to save, it limits
Its application.Hawthorn it is existing it is further exploitation have hawthorn red wine (CN104877859A), haw fruit vinegar (CN107439878A),
Hawthorn beverage (CN107467432A), hawthorn ferment (CN105852090A), but these products are to nutriment ingredient in raw material
Extraction be not enough.
Summary of the invention
In view of this, solving biography the purpose of the present invention is to provide a kind of hawthorn probiotics ferment and preparation method thereof
The narrow problem of system hawthorn application approach.
The technical scheme adopted by the invention is as follows:
A kind of preparation method of hawthorn probiotics ferment, comprising the following steps:
Hair is added in fresh hawthorn 500-700kg, Chinese yam 200-300kg, Fructus Corni 100-200kg, brown granulated sugar 50kg by S1
In ferment equipment；
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 10-25g into Zymolysis Equipment and carries out just fermentation, when fermentation
Between be 5-7 months；Lactobacillus plantarum 10-15g, Lactobacillus rhamnosus 10-15g are just added after fermentation and carries out second of hair
Ferment, fermentation time 7-9 months；Lactobacillus acidophilus 5-10g is added after fermentation for the second time and continues third time fermentation 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.8-3.5, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain hawthorn probiotics enzyme stoste.
When just fermentation, second of fermentation and third time are fermented in the S2, isolation air carries out anaerobic fermentation.
Zymolysis Equipment is fermenter.
Using hawthorn probiotics ferment made from method made above.
The progress of hawthorn probiotics enzyme stoste is filling to obtain finished product.
Filling condition: control Cleanliness reaches 100,000 grades of prescribed requirements, shop worker now washes your hands before entering workshop,
It changes one's clothes, sterilize, strictly operated by the operating instruction of bottle placer and Cover whirling Machine, the filling amount check at any time in filling progress,
The links such as seal quality, it is ensured that product complies with standard technical requirements.Before filling the container afterwards by equipment and pipeline carry out thoroughly cleaning and
Disinfection；It is filling to be bottled, bottle and bottle cap cleaning: bottle first uses pure water reverse-flush, then is dried up with clean compressed air, lid
It is carried out disinfection processing with disinfection cabinet, guarantee reaches sterility requirements.
It is preferred that following technical scheme: in S2, fermenting bucket is 850, and each 1200 liters of fermentation ladle capacity (adds every time
Enter raw material total amount 1000kg or so), electronic scale 2,1 ton of every fermenter charge, fermentation temperature is room temperature, and workshop sealing is protected
Cleaning, normal temperature fermentation are held, fermentation time is 24 months.
In S5, standing purifying tank is 50, and it is food grade plastic using material that each standing purifying tankage size, which is 30 tons,
Material.
When filling, the model TD-8 of bottle placer, the quantity of bottle placer is 2 sets；The model TD-SP1 of Cover whirling Machine, Cover whirling Machine
Quantity be 2 sets, filling speed be 2000 bottles/hour, bottle device be 30ml vial.
When bottle and bottle cap clean, bottle device 2 are managed；Full-automatic inverting bottle cleaning machine 2, the model of full-automatic inverting bottle cleaning machine
CP-15；Disinfection cabinet 1, the model ZTP-390 of disinfection cabinet.
Compared with prior art, the method have the benefit that:
The present invention using hawthorn, Chinese yam, Fructus Corni as primary raw material, the nutritional ingredient of raw material in scientific and reasonable collocation more
Add abundant, water is not added in preparation process, nutriment and activity substance content, concentration in ferment have sufficiently been ensured, in benefit
Under the fermentation of raw bacterium, while the type of strict control probiotics, dosage and fermentation duration, guarantee the fermentation of raw material more
Sufficiently, depth, fermentation process are divided into three phases and are separately added into different strains according to the characteristic of different phase, have fermentation
Have levels more rationally；Especially secondary fermentation stage, spontaneous fermentation, are not further added by sugar source, finally make the nutrition in raw material
Substance, active material, antioxidant can discharge to decomposite completely to be come, and is improved existing ferment using raw material and is added water by fermentation
Or ferment fermentation efficiency caused by the production method of fruit juice fermentation is low, active material, nutrient composition content are low and health care is imitated
The technical problems such as fruit is not significant.It is prebiotic to finally obtain a kind of good mouthfeel, full of nutrition, spleen benefiting and stimulating the appetite, the hawthorn of relieving dyspepsia
Bacterium ferment increases hawthorn probiotics ferment nutritive value.
Hawthorn probiotics ferment produced by the present invention is free of any food additives, safe and healthy, has no toxic side effect, can put
The heart is enjoyed, and various people are suitable for；Solve that traditional hawthorn application approach is narrow, product form Single-issue.
Specific embodiment
Illustrate a specific embodiment of the invention below with reference to embodiment, but following embodiment is used only to be described in detail
The present invention does not limit the scope of the invention in any way.
Raw material is added before fermenter through over cleaning, drying, ultraviolet disinfection, crushing in following embodiment.
A kind of embodiment 1: preparation method of hawthorn probiotics ferment, comprising the following steps:
Fresh hawthorn 650kg, Chinese yam 200kg, Fructus Corni 100kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 10g and lactobacillus fermenti 25g into fermenter and carries out just fermentation, and fermentation time is 6
Month；Lactobacillus plantarum 15g, Lactobacillus rhamnosus 15g are just added after fermentation ferment for the second time, fermentation time 8 months；
Lactobacillus acidophilus 10g is added after fermentation for the second time and continues third time fermentation 8 months；
When just fermentation, second of fermentation and third time are fermented, fermenter overlay film, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.8-3.5, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain hawthorn probiotics enzyme stoste.
A kind of embodiment 2: preparation method of hawthorn probiotics ferment, comprising the following steps:
Fresh hawthorn 700kg, Chinese yam 200kg, Fructus Corni 100kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 5g and lactobacillus fermenti 25g into fermenter and carries out just fermentation, and fermentation time is 5
Month；Lactobacillus plantarum 10g, Lactobacillus rhamnosus 15g are just added after fermentation ferment for the second time, fermentation time 9 months；
Lactobacillus acidophilus 5g is added after fermentation for the second time and continues third time fermentation 7 months；
When just fermentation, second of fermentation and third time are fermented, fermenter overlay film, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.8-3.5, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain hawthorn probiotics enzyme stoste.
A kind of embodiment 3: preparation method of hawthorn probiotics ferment, comprising the following steps:
Fresh hawthorn 500kg, Chinese yam 300kg, Fructus Corni 200kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 10g and lactobacillus fermenti 10g into fermenter and carries out just fermentation, and fermentation time is 7
Month；Lactobacillus plantarum 15g, Lactobacillus rhamnosus 10g are just added after fermentation ferment for the second time, fermentation time 7 months；
Lactobacillus acidophilus 8g is added after fermentation for the second time and continues third time fermentation 9 months；
When just fermentation, second of fermentation and third time are fermented, fermenter overlay film, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.8-3.5, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain hawthorn probiotics enzyme stoste.
A kind of embodiment 4: preparation method of hawthorn probiotics ferment, comprising the following steps:
Fresh hawthorn 600kg, Chinese yam 250kg, Fructus Corni 150kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 8g and lactobacillus fermenti 20g into fermenter and carries out just fermentation, and fermentation time is 6
Month；Lactobacillus plantarum 12g, Lactobacillus rhamnosus 12g are just added after fermentation ferment for the second time, fermentation time 8 months；
Lactobacillus acidophilus 8g is added after fermentation for the second time and continues third time fermentation 8 months；
When just fermentation, second of fermentation and third time are fermented, fermenter overlay film, isolation air carries out tri- rank of anaerobic fermentation S3
After fermentation, detection pH stablizes between 2.8-3.5 section, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stand purifying: by filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain hawthorn probiotics enzyme stoste.
After embodiment 1-4 obtains hawthorn probiotics enzyme stoste, microbiological indicator, again need to be detected by national standard GB/T31121
Metal indicator is up to standard, other indexs reach professional standard T/CBFIA08003-2016, and then hawthorn probiotics enzyme stoste is through special
It is piped to racking room, it is filling to obtain hawthorn probiotics ferment finished product.
Effect experiment: the relieving dyspepsia function of hawthorn probiotics ferment obtained in embodiment 1-4 is verified
Experimental subjects: healthy SPF grades of male SD rat, weight (200 ± 20g)
Experiment reagent and instrument
Carboxymethyl cellulose；Trophism semisolid paste (self-control): taking 5g carboxymethyl cellulose to be dissolved in 100ml distilled water,
It is separately added into milk powder 8g, white sugar 4g, starch 4g, 2ml carbon black ink and distilled water, semisolid paste 150ml is made into, is placed in refrigerator
It is stored refrigerated, it is taken out using preceding 2h, restores room temperature；Rat GAD Elisa kit；Rat SS Elisa kit；Rat SP
Elisa kit；Electronic balance；Low speed centrifuge；Absorb light microplate reader.It is other: Mosapride；Wild cabbage；Refined lard；
Experimental modeling
Swimming method is exhausted using intemperance of taking food+power and establishes functional dyspepsia FD Spleen Rats, by observing sample
The effect of relieving dyspepsia is verified to rat gastric emptying rate.
Modeling: even-numbered days ad lib water, feeding refined lard (1.5ml/100g) 1 time, the odd-numbered day is deprived of food but not water, and is fed with sweet
Blue 10-15g/, to reach eating and drinking without temperance；And rat is placed in the odd-numbered day and fills warm water (22 ± 1 DEG C of water temperature), depth of water 50cm's
Plastic barrel went swimming to power exhausts (to be rotated, sports coordination is decreased obviously, water submerged nose, body sinking, until again in rat water
Emerge more than 10s, continuous 3 times) 1 time, to reach overstrain, total 25d.Rat appearance activity is reduced, tired crouch is moved less, pricks
Heap narrows eye, hogback, slow in reacting, dry skin and hair and owes gloss, revolts powerless when arresting, and feed reduces, thin and weak, body of gradually seeing figure
Decline again or increasess slowly, soft stool or loose stool, modeling success.
Grouping and processing
Rat adaptive feeding after a week, is randomly divided into blank control group, model control group, Mosapride group and experimental group
1-4, every group 15.Blank control group is normal raising rat, and other each groups are the functional dyspepsia FD after modeling successfully
Rats with Spleen-deficiency；Blank control group and model control group periodically give stomach-filling 7.2ml/ (kg.d) physiological saline daily；Experimental group 1-4
Group periodically gives hawthorn probiotics ferment sample obtained in stomach-filling 7.2ml/ (kg.d) embodiment 1-4 daily；Continue stomach-filling 1
Week, stomach-filling condition, rearing conditions are identical.
Observation index and method:
Gastric emptying rate measurement: last gives semisolid paste 2ml stomach-filling, the appropriate sodium of sulfuric acid after 1h to can't help water after sample afterwards for 24 hours
(40ml/kg) intraperitoneal injection anesthesia, opens rapidly abdominal cavity, ligatures cardia and pylorus immediately, and clip stomach claims stomach total quality, edge
Greater curvature cuts off body of stomach, and after washing away gastric content, the net quality of stomach is claimed after wiping.
By formula: gastric emptying rate=1- [(the stomach total quality-net quality of stomach)/stomach-filling quality] × 100%
Statistical procedures data withIt indicates, using one-way analysis of variance, is counted with 20.0 software of SPSS
Credit analysis, P < 0.05 are that difference is statistically significant.
Each group rat gastric emptying rate compares
| Group | Number of elements (only) | Gastric emptying rate (%) |
| Blank control group | 15 | 70.68±12.37 |
| Model control group | 15 | 46.98±16.57** |
| Mo Shalibi group | 15 | 67.16±13.95# |
| Experimental group 1 | 15 | 60.58±17.10## |
| Experimental group 2 | 15 | 60.44±15.92## |
| Experimental group 3 | 15 | 59.89±16.32## |
| Experimental group 4 | 15 | 60.21±17.03## |
Note: * * is compared with blank control group, P < 0.01；## is compared with model control group, P < 0.05；# and model comparison
Group compares P < 0.01
Compared with blank control group, model control group gastric emptying rate significantly reduces (P < 0.01), demonstrates functional digestive
Bad Spleen Rats are successfully established；Compared with model control group, gastric emptying rate is aobvious for Mosapride group, experimental group 1-4 group
It writes and is higher than model control group, wherein Mo Shalibi group P < 0.01 compared with model control group, experimental group 1-4 group model control group
Compared to P < 0.05；Demonstrating hawthorn probiotics ferment obtained by embodiment 1-4 has significant relieving dyspepsia function, wherein
It is the most significant with hawthorn probiotics ferment effect obtained by embodiment 1.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, this field is common
Other modifications or equivalent replacement that technical staff makes technical solution of the present invention, without departing from technical solution of the present invention
Spirit and scope, be intended to be within the scope of the claims of the invention.
Claims (4)
1. a kind of preparation method of hawthorn probiotics ferment, which comprises the following steps:
Fresh hawthorn 500-700kg, Chinese yam 200-300kg, Fructus Corni 100-200kg, brown granulated sugar 50kg are added fermentation and set by S1
In standby；
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 10-25g into Zymolysis Equipment and just ferment, and fermentation time is
5-7 months；Lactobacillus plantarum 10-15g, Lactobacillus rhamnosus 10-15g are just added after fermentation and carries out second of fermentation, hair
Ferment time 7-9 month；Lactobacillus acidophilus 5-10g is added after fermentation for the second time and continues third time fermentation 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 2.8-3.5, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying: filtered fermentation liquid being stood and is purified, as alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106CFU/
It purifies and completes when ml, obtain hawthorn probiotics enzyme stoste.
2. the preparation method of hawthorn probiotics ferment according to claim 1, it is characterised in that: just fermentation, the in the S2
When secondary fermentation and third time are fermented, isolation air carries out anaerobic fermentation.
3. the preparation method of hawthorn probiotics ferment according to claim 1, it is characterised in that: Zymolysis Equipment is fermentation
Bucket.
4. using hawthorn probiotics ferment made from any one of claim 1-3 preparation method.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112190644A (en) * | 2019-07-08 | 2021-01-08 | 大江生医股份有限公司 | Plant fermentation product, preparation method thereof and application of plant fermentation product in stomach health care |
| CN112691155A (en) * | 2020-05-28 | 2021-04-23 | 江苏蓝果临床营养科技有限公司 | A Chinese medicinal composition for the comprehensive treatment of stomach diseases |
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| CN106578794A (en) * | 2016-12-01 | 2017-04-26 | 李恒 | Fermented fruit and vegetable juice beverage preparation process |
| CN106617051A (en) * | 2016-11-21 | 2017-05-10 | 郑州市嘉蓓丽生物科技有限公司 | Fruit and vegetable ferment freeze-dried powder and preparation method thereof |
| CN107348272A (en) * | 2017-06-01 | 2017-11-17 | 扬生（南召）生物科技有限公司 | A kind of probiotics fruits and vegetables enzyme beverage and preparation method thereof |
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| CN106617051A (en) * | 2016-11-21 | 2017-05-10 | 郑州市嘉蓓丽生物科技有限公司 | Fruit and vegetable ferment freeze-dried powder and preparation method thereof |
| CN106578794A (en) * | 2016-12-01 | 2017-04-26 | 李恒 | Fermented fruit and vegetable juice beverage preparation process |
| CN107348272A (en) * | 2017-06-01 | 2017-11-17 | 扬生（南召）生物科技有限公司 | A kind of probiotics fruits and vegetables enzyme beverage and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112190644A (en) * | 2019-07-08 | 2021-01-08 | 大江生医股份有限公司 | Plant fermentation product, preparation method thereof and application of plant fermentation product in stomach health care |
| CN112691155A (en) * | 2020-05-28 | 2021-04-23 | 江苏蓝果临床营养科技有限公司 | A Chinese medicinal composition for the comprehensive treatment of stomach diseases |
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