CN108835624A - A kind of blueberry probiotics ferment and preparation method thereof - Google Patents
A kind of blueberry probiotics ferment and preparation method thereof Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 109
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 53
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 53
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 53
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 45
- 239000006041 probiotic Substances 0.000 title claims abstract description 40
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 93
- 241000287420 Pyrus x nivalis Species 0.000 claims abstract description 7
- 230000001476 alcoholic effect Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 238000002955 isolation Methods 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 8
- 244000077233 Vaccinium uliginosum Species 0.000 claims description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
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- 239000004310 lactic acid Substances 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
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- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
Abstract
The invention belongs to ferment production technical fields, and in particular to a kind of blueberry probiotics ferment and preparation method thereof.The blueberry probiotics ferment is using blueberry, pyrus nivalis, orange as primary raw material, the nutritional ingredient of raw material is more abundant in scientific and reasonable collocation, under the fermentation of probiotics, the type, dosage of strict control probiotics and fermentation duration simultaneously, guarantee that the fermentation of raw material is more abundant, finally enable the nutriment in raw material, active material, antioxidant to release completely, finally obtains a kind of rich in nutrition content, there is improving eyesight to sober up, the blueberry probiotics ferment of resolving sputum detoxicating functions.
Description
Technical field
The invention belongs to ferment production technical fields, and in particular to a kind of blueberry probiotics ferment and preparation method thereof.
Background technique
Ferment, which refers to, ferments to fruit and vegetable by microorganism, a kind of liquid containing bioactive ingredients of extraction.Above-mentioned life
Object active constituent includes enzyme but is not limited to enzyme, including active antioxidants, biomass element etc., from fermentation participate in bacterium and
Food materials for fermentation.
The ferment rationally made, the active substance of plant extracted are from food materials, and participation bacterium of fermenting is even more people
The component part of body microecosystem.Therefore, the side effect of ferment is almost nil.Powerful effect, minimum side effect, in recent years
Come always in explosive increase trend.
Nutritional ingredient rich in Blueberry, having prevents cranial nerve aging, protection eyesight, heart tonifying, anticancer, soft
Change blood vessel, the functions such as enhance the immune of the body, nutritional ingredient is high.Blueberry is rich in anthocyanidin, has effects that activation retina, can be with
Strengthen eyesight, prevents eyeball fatigue and gazed at.Blueberry storage time is shorter, once excess capacity occurs in market, market disappears
Change, which is difficult to save not the blueberry fallen, to be discarded.The existing further exploitation of blueberry has blueberry wine (CN104974914A), indigo plant
Certain kind of berries beverage (CN104223221A), blueberry ferment (CN105815768A), but these products all not too much fill the fermentation of raw material
Point.
Summary of the invention
In view of this, solving biography the purpose of the present invention is to provide a kind of blueberry probiotics ferment and preparation method thereof
The narrow problem of system blueberry application approach.
The technical scheme adopted by the invention is as follows：
A kind of preparation method of blueberry probiotics ferment, includes the following steps：
Zymolysis Equipment is added in blueberry 550-700kg, pyrus nivalis 150-200kg, orange 50-200kg, brown granulated sugar 50kg by S1
In；
S2 continuously adds saccharomycete 5-10g and lactobacillus paracasei 1-5g into Zymolysis Equipment and carries out just fermentation, when fermentation
Between be 5-7 months；Lactobacillus plantarum 1-5g, lactobacillus bulgaricus 1-5g are just added after fermentation and carries out second of fermentation,
Fermentation time 7-9 months；Lactobacillus acidophilus 5-10g, lactobacillus reuteri 1-5g is added after fermentation for the second time and continues third
Secondary fermentation 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 3.0-3.9, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying：By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain blueberry probiotics enzyme stoste.
When just fermentation, second of fermentation and third time are fermented in the S2, isolation air carries out anaerobic fermentation.
Zymolysis Equipment is fermenter.
Using blueberry probiotics ferment made from method made above.
The progress of blueberry probiotics enzyme stoste is filling to obtain finished product.
Filling condition：Control Cleanliness reaches 100,000 grades of prescribed requirements, shop worker now washes your hands before entering workshop,
It changes one's clothes, sterilize, strictly operated by the operating instruction of bottle placer and Cover whirling Machine, the filling amount check at any time in filling progress,
The links such as seal quality, it is ensured that product complies with standard technical requirements.Before filling the container afterwards by equipment and pipeline carry out thoroughly cleaning and
Disinfection；Filling is bottled, bottle and bottle cap cleaning：Bottle first uses pure water reverse-flush, then is dried up with clean compressed air, lid
It is carried out disinfection processing with disinfection cabinet, guarantee reaches sterility requirements.
It is preferred that following technical scheme：In S2, fermenting bucket is 850, and each 1200 liters of fermentation ladle capacity (adds every time
Enter raw material total amount 1000kg or so), electronic scale 2,1 ton of every fermenter charge, fermentation temperature is room temperature, and workshop sealing is protected
Cleaning, normal temperature fermentation are held, fermentation time is 24 months.
In S5, standing purifying tank is 50, and it is food grade plastic using material that each standing purifying tankage size, which is 30 tons,
Material.
When filling, the model TD-8 of bottle placer, the quantity of bottle placer is 2 sets；The model TD-SP1 of Cover whirling Machine, Cover whirling Machine
Quantity be 2 sets, filling speed be 2000 bottles/hour, bottle device be 30ml vial.
When bottle and bottle cap clean, bottle device 2 are managed；Full-automatic inverting bottle cleaning machine 2, the model of full-automatic inverting bottle cleaning machine
CP-15；Disinfection cabinet 1, the model ZTP-390 of disinfection cabinet.
Compared with prior art, the method have the benefit that：
The present invention using blueberry, pyrus nivalis, orange as primary raw material, the nutritional ingredient of raw material in scientific and reasonable collocation more
It is abundant, under the fermentation of probiotics, while the type of strict control probiotics, dosage and fermentation duration, guarantee raw material
Fermentation it is more abundant, fermentation process is divided into three phases and is separately added into different strains according to the characteristic of different phase, makes
Fermentation has level more reasonable；Especially secondary fermentation stage, spontaneous fermentation are not further added by sugar source, can consume original in this way
Remaining sugar in fermentation liquid, sufficiently consumption carbohydrate, finally make nutriment, the active material, polyphenoils mass-energy in raw material
It is enough to release completely, it finally obtains a kind of rich in nutrition content, there is improving eyesight to sober up, the blueberry probiotics ferment of resolving sputum detoxicating functions
Element increases blueberry probiotics ferment nutritive value.
Blueberry probiotics ferment produced by the present invention is free of any food additives, safe and healthy, has no toxic side effect, can put
The heart is enjoyed, and various people are suitable for；Solve that traditional blueberry application approach is narrow, product form Single-issue.
Specific embodiment
Illustrate a specific embodiment of the invention below with reference to embodiment, but following embodiment is used only to be described in detail
The present invention does not limit the scope of the invention in any way.
Raw material is added before fermenter through over cleaning, drying, ultraviolet disinfection, crushing in following embodiment.
Embodiment 1：A kind of preparation method of blueberry probiotics ferment, includes the following steps：
Blueberry 700kg, pyrus nivalis 150kg, orange 100kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 10g and lactobacillus paracasei 5g into fermenter and carries out just fermentation, fermentation process fermentation
Bucket overlay film, isolation air carry out anaerobic fermentation, and fermentation time is 6 months；Lactobacillus plantarum 5g is just added after fermentation, guarantor adds
Leah lactobacillus 5g carries out second and ferments, and fermentation process fermenter overlay film, isolation air carries out anaerobic fermentation, fermentation time 8
A month；Lactobacillus acidophilus 10g, lactobacillus reuteri 5g is added after fermentation for the second time and continues third time fermentation, fermentation process
Fermenter overlay film, isolation air progress anaerobic fermentation, fermentation time 8 months；
After tri- stage fermentation of S3, detection pH stablizes between 3.0-3.9, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying：By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain blueberry probiotics enzyme stoste.
Using blueberry probiotics ferment made from method made above.
Embodiment 2：A kind of preparation method of blueberry probiotics ferment, includes the following steps：
Blueberry 550kg, pyrus nivalis 200kg, orange 200kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 5g and lactobacillus paracasei 5g into fermenter and carries out just fermentation, fermentation process fermenter
Overlay film, isolation air carry out anaerobic fermentation, and fermentation time is 5 months；Lactobacillus plantarum 1g, Bao Jiali are just added after fermentation
Sub- lactobacillus 5g carries out second and ferments, fermentation process fermenter overlay film, isolation air progress anaerobic fermentation, and fermentation time 9
Month；Lactobacillus acidophilus 5g, lactobacillus reuteri 5g is added after fermentation for the second time and continues third time fermentation, fermentation process fermentation
Bucket overlay film, isolation air progress anaerobic fermentation, fermentation time 7 months；
After tri- stage fermentation of S3, detection pH stablizes between 3.0-3.9, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying：By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain blueberry probiotics enzyme stoste.
Using blueberry probiotics ferment made from method made above.
Embodiment 3：A kind of preparation method of blueberry probiotics ferment, includes the following steps：
Blueberry 600kg, pyrus nivalis 180kg, orange 100kg, brown granulated sugar 50kg are added in fermenter S1；
S2 continuously adds saccharomycete 10g and lactobacillus paracasei 1g into fermenter and carries out just fermentation, fermentation process fermentation
Bucket overlay film, isolation air carry out anaerobic fermentation, and fermentation time is 7 months；Lactobacillus plantarum 5g is just added after fermentation, guarantor adds
Leah lactobacillus 1g carries out second and ferments, and fermentation process fermenter overlay film, isolation air carries out anaerobic fermentation, fermentation time 7
A month；Lactobacillus acidophilus 10g, lactobacillus reuteri 1g is added after fermentation for the second time and continues third time fermentation, fermentation process
Fermenter overlay film, isolation air progress anaerobic fermentation, fermentation time 9 months；
After tri- stage fermentation of S3, detection pH stablizes between 3.0-3.9, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying：By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 ×
106It purifies and completes when CFU/ml, obtain blueberry probiotics enzyme stoste.
Using blueberry probiotics ferment made from method made above.
After embodiment 1-3 obtains blueberry probiotics enzyme stoste, microbiological indicator, again need to be detected by national standard GB/T31121
Metal indicator is up to standard, other indexs reach professional standard T/CBFIA08003-2016, and then blueberry probiotics enzyme stoste is through special
It is piped to racking room, it is filling to obtain blueberry probiotics ferment finished product.
Effect experiment：Dispelling effects of alcohol verifying to blueberry probiotics ferment obtained in embodiment 1-3
Experimental modeling establishes alcoholic liver injury in rats model
Experimental subjects：Full nutrition pellet is freely eaten in weight 125-155g male SD rat
By alcohol by volume score be 0.52 Red Star strong, colourless liquor distilled from sorghum from wine (BJ Seal Wine General Factory's production), it is dilute respectively by volume
It is spare to be interpreted into 400Io, 450Io, 500Io (v/v).
Modeling method：Rat each 1 time of white wine stomach-filling daily morning and evening is given by weight measured weekly.It will be diluted within 1st week
40% white wine according to dosage 4g/ (kg.d), each 0.5m1/100g stomach-filling, the 2nd week according to dosage 8g/ (kg.d), each 1.0ml/
100g, stomach-filling, start within the 5th week will to be diluted to 45% from wine by 9g/ (kg.d), each 1.0m1/100g stomach-filling the 9th week will be dilute
50% white wine is interpreted by 10g/ (kg.d), each 1.0m1/100g stomach-filling, uses instead and is freely drunk to the 12nd week from the 11st week, with
50% white wine of concentration is as main beverage, day supply amount 40ml, while water restriction.Dead rat is discarded after 12 weeks, as
Rat model.
Experiment content
It with rat 80 of the raising of above-mentioned experimental model condition, is fed, free water, is adapted to rat full-valence pellet feed
Property raising 3d, be randomly divided into 4 groups, every group 20 by weight.
Rat Fast (can't help water) 6h before testing, each component also known as weigh sb., and give edible wine (alcohol by volume point by weight
Number gives control group from after wine (BJ Seal Wine General Factory's production) 7.5ml/kg stomach-filling, 30min for 0.52 Red Star strong, colourless liquor distilled from sorghum respectively
Obtained blueberry probiotics ferment 0.7g/kg stomach-filling in physiological saline 0.7g/kg, experimental group 1-3 corresponding embodiment 1-4.In giving
After sample 1h and 12h, the blood sampling of rat eye socket.10min is centrifuged with 3500r/min after blood standing 30min, upper serum is taken to measure second
Determining alcohol.
Shadow of the obtained blueberry probiotics ferment to concentration of alcohol in alcoholic liver injury in rats serum in embodiment 1-3
It ringsμl/100ml
| Group | n | To concentration of alcohol after sample 1h | To concentration of alcohol after sample 12h |
| Control group | 20 | 593.2±161.3 | 10.9±4.6 |
| Experimental group 1 | 20 | 402.6±139.31) | 5.1±2.51) |
| Experimental group 2 | 20 | 510.6±164.2 | 9.2±3.8 |
| Experimental group 3 | 20 | 422.0±172.22) | 5.6±5.62) |
Note：Compared with the control group1)P < 0.01,2)P < 0.05
Experimental group 1-3 show blueberry probiotics ferment obtained by embodiment 1-3 for alcoholic liver injury in rats with it is right
It compares to have to alcohol levels in blood samples concentration after sample 1h, 12h according to group and more be remarkably decreased, it was demonstrated that the benefit of blueberry obtained by embodiment 1-3
Raw bacterium ferment has preferable dispelling effects of alcohol, and the most significant with blueberry probiotics ferment effect obtained by embodiment 1.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, this field is common
Other modifications or equivalent replacement that technical staff makes technical solution of the present invention, without departing from technical solution of the present invention
Spirit and scope, be intended to be within the scope of the claims of the invention.
Claims (4)
1. a kind of preparation method of blueberry probiotics ferment, which is characterized in that include the following steps：
Blueberry 550-700kg, pyrus nivalis 150-200kg, orange 50-200kg, brown granulated sugar 50kg are added in Zymolysis Equipment S1；
S2 continuously adds saccharomycete 5-10g and lactobacillus paracasei 1-5g into Zymolysis Equipment and just ferment, and fermentation time is
5-7 months；Lactobacillus plantarum 1-5g, lactobacillus bulgaricus 1-5g are just added after fermentation and carries out second of fermentation, fermentation
Time 7-9 month；Lactobacillus acidophilus 5-10g, lactobacillus reuteri 1-5g is added after fermentation for the second time and continues third time hair
Ferment 7-9 months；
After tri- stage fermentation of S3, detection pH stablizes between 3.0-3.9, and when alcoholic strength < 0.5%, fermentation is completed；
The fermentation liquid that fermentation is completed is filtered by S4；
S5 stands purifying：Filtered fermentation liquid is stood and is purified, as alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106CFU/
It purifies and completes when ml, obtain blueberry probiotics enzyme stoste.
2. the preparation method of blueberry probiotics ferment according to claim 1, it is characterised in that：Just fermentation, the in the S2
When secondary fermentation and third time are fermented, isolation air carries out anaerobic fermentation.
3. the preparation method of blueberry probiotics ferment according to claim 1, it is characterised in that：Zymolysis Equipment is fermentation
Bucket.
4. using blueberry probiotics ferment made from any one of claim 1-3 preparation method.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142447A (en) * | 2021-04-20 | 2021-07-23 | 南京益唯森生物科技有限公司 | Nitraria tangutorum bobr ferment and preparation method thereof |
| CN113287699A (en) * | 2021-04-28 | 2021-08-24 | 南京益唯森生物科技有限公司 | Elaeagnus angustifolia enzyme and preparation process thereof |
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| CN113287699A (en) * | 2021-04-28 | 2021-08-24 | 南京益唯森生物科技有限公司 | Elaeagnus angustifolia enzyme and preparation process thereof |
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