CN109232107A - Yeast selenium foliar fertilizer, production method and application - Google Patents

Yeast selenium foliar fertilizer, production method and application Download PDF

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Publication number
CN109232107A
CN109232107A CN201811377646.6A CN201811377646A CN109232107A CN 109232107 A CN109232107 A CN 109232107A CN 201811377646 A CN201811377646 A CN 201811377646A CN 109232107 A CN109232107 A CN 109232107A
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China
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parts
yeast
foliar fertilizer
selenium
fertilizer
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CN201811377646.6A
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陈红麟
张有利
孙立影
李鑫
战鑫
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Heilongjiang Animal Health And Animal Husbandry LLC
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Heilongjiang Animal Health And Animal Husbandry LLC
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Priority to CN201811377646.6A priority Critical patent/CN109232107A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/27Dispersions, e.g. suspensions or emulsions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

This application discloses a kind of yeast selenium foliar fertilizer, production method and applications, production yeast selenium blade face fertilizer suspending agent includes the raw material of following parts by weight: soluble Se-enriched yeast 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, and 1-20 parts of synergist, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus are water;Production yeast selenium foliar fertilizer can wet-milling include following parts by weight raw material: soluble Se-enriched yeast 10-60 part, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.Yeast selenium foliar fertilizer of the invention it is toxic it is small, environmental pollution is small;Yeast selenium foliar fertilizer of the present invention cooperates synergist, keeps the fertilizer efficiency of foliar fertilizer long, and synergist promotes the adhesion strength of foliar fertilizer drop;Heavy metal in degradable crops;It reduces hard water ions and the adverse effect such as antagonism is generated to effective component, be conducive to the performance of fertilizer fertilizer;And increase the disease resistance of crops.

Description

Yeast selenium foliar fertilizer, production method and application
Technical field
Present invention crop technical field of fertilizers concerning farmers, specifically, more particularly to a kind of yeast selenium foliar fertilizer, production method And application.
Background technique
Selenium is the element of glutathione peroxidase (GSH-P) in humans and animals body, containing about 4mol in every pure enzyme of 1mol Selenium.GSH-Px is first putative selenoenzyme in organism.GSH-P be widely present in the liver of viable organism, kidney, the heart, Lung etc., selenocysteine provide the necessary gene in its activated centre for the enzyme, and GSH-P makes virose mistake using glutathione Oxide is reduced to harmless hydroxy compounds, decomposes peroxide, scavenging capacity free radical position, protects membrane structure And function, repair molecular damage.Selenium has the immunity that body much can be improved to the benefit of human body, removes free radical, degrades Cancer morbidity, protection liver, prevention diabetes and removing toxic substances.As the improvement of people's living standards, health of the people to oneself It focuses more on, the demand to selenium-enriched food also starts more and more.Biological selenium-enriched is the effective way for obtaining selenium, by absorbing vegetable Selenium element in dish fruit is the important way that human body can obtain selenium.And the natural Se content in vegetable and fruit is less, it can be with Increase Se content in agricultural product by fertilizer of the application containing selenium element.Wherein, the method for fertilising can be divided into two kinds, first is that It is applied fertilizer by soil, but the fertilizer efficiency of this method fertilising is long, nutrient absorption is few, and will cause injury to soil;Separately It one is foliar fertilizer, is absorbed by blade face, is not influenced by the variation of extraneous unfavorable conditions.Most selenium fertilizer is at present It adds made of selenite or selenium-rich ore, this inorganic selenium uses the malicious vice president of human body, especially to the serious thorn of stomach Swash.Se-enriched yeast is that certain density inorganic selenium source is added in saccharomycete incubation, and inorganic selenium is endobacillary with yeast Protein organically combines inorganic selenium of the selenoprotein toxicity at selenoprotein, after conversion well below chemical state.And yeast selenium Toxicity is also far below chelating selenium.Food nonhazardous containing yeast selenium, is free from side effects safely.In numerous Selenium supplement agents, yeast selenium Contained in machine Se content it is high, and the organic selenium activity in Se-enriched yeast is higher than inorganic selenium.
It selects foliar fertilizer to have many benefits, can be used together with insecticide with fungicide, be that pretty economical is effective Operating method;Leaf portion nutrition is supplemented using foliar fertilizer, high financial profit saves fertilizer;Because it is effective to directly feed flowers and trees Nutrient solves soil and fixes to the absorption of nutrient;The operating of crops Nutrient Absorption is fast, and utilization rate is high, can meet needs in time; Use environment requires not tight.But existing foliar fertilizer, because after the cuticula of blade face, Foliage Absorption nutrient penetrance is low.And it easily flows It loses, during spraying, if misoperation or chance strong wind weather, easily low from blade face, wastes medical fluid;The chance easy leaching loss of rain, one As in the case of, 4 hours chance rain need to apply fertilizer again after spray;If weather overdrying, medical fluid is dried rapidly image on blade face and is absorbed, And then fertilizer efficiency.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of yeast selenium foliar fertilizers, which is characterized in that including following parts by weight Raw material, wherein soluble Se-enriched yeast 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, 1-20 parts of synergist, defoaming 0.1-1 parts of agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus are water.
Preferably, the raw material including following parts by weight, wherein soluble Se-enriched yeast 20-50 parts, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, 5-15 parts of synergist, 0.3-0.8 parts of defoaming agent, 3-8 parts of antifreeze, 1-2 parts of thickener, surplus are water.
A kind of yeast selenium foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Se-enriched yeast 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.
Preferably, the raw material including following parts by weight, wherein soluble Se-enriched yeast 20-50 parts, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, surplus are anhydrous sodium sulphate.
Preferably, the synergist includes phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene One or more mixtures of ether compound.
Preferably, the wetting agent includes: naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fat One of acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures;
The dispersing agent includes: one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion Or a variety of mixtures;
The thickener includes: polysaccharide compound.
Yeast selenium foliar fertilizer production method, which is characterized in that include the steps that making soluble Se-enriched yeast:
Three-level activates Ruan's Candida, and add Selenium Sulphate in the medium in activation process is to ultimate density respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL obtain seed culture fluid;
According to 1-3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25-35 in fermentation temperature DEG C, tank pressure is 0.02-0.07Mpa, ventilatory capacity 500-700m320-30h is cultivated under conditions of/h, obtains fermentation liquid;The hair Contain 2% glucose, 1%NH in ferment culture medium4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4、 0.1%Na2HPO4, 0.1%MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 25-35min is centrifuged in the case where revolving speed is 2500-3500rpm and is cleaned with clear water, is so repeated 3 times, Centrifugation gained is deposited under 100-200MPa and handles 20-30min, obtains the soluble Se-enriched yeast.
Preferably, further include the steps that making yeast selenium blade face fertilizer suspending agent:
Soluble 10-60 parts of the Se-enriched yeast is taken, and synergist 1-20 parts is added thereto, 1-30 parts of wetting agent, divides 1-30 parts of powder, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus are water, are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5-7.2, obtains the yeast selenium foliar fertilizer and suspends Agent.
Preferably, further including the steps that making yeast selenium foliar fertilizer can wet-milling:
Soluble 10-60 part of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain air-drying selenium-rich afterwards Yeast;
1-30 parts of wetting agents, 1-30 parts of dispersing agents, anhydrous sodium sulphate and the air-dried rear Se-enriched yeast are mixed, mixture is put Enter progress level-one mixing in raw material mixing machine, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
The application of yeast selenium foliar fertilizer.
Compared with prior art, yeast selenium foliar fertilizer, production method and application provided by the invention reach following beneficial to effect Fruit:
The first, yeast selenium foliar fertilizer of the invention has small toxicity, environmental pollution by the way that inorganic selenium is converted to organic selenium The advantages that small, bioactivity is high and easy absorption, and the toxicity of yeast selenium is also far below chelating selenium, and the food containing yeast selenium is nontoxic Evil, is free from side effects safely.
The second, the Se content in yeast selenium of the present invention is high, and cooperation synergist keeps the fertilizer efficiency of yeast selenium foliar fertilizer of the present invention long, Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs selenium element, improves the Se content of crops.
Heavy metal in the degradable crops of Se-enriched yeast in third, the present invention, makes crop yield increase.
4th, the present invention promotes the adhesion strength of foliar fertilizer drop by the way that synergist is added, and greatly prevents blade face solution Drop evaporates because environmental factor is low from blade face, greatly reduces the waste of fertilizer.
5th, the present invention is by being added synergist, it is possible to reduce hard water ions generate the unfavorable shadows such as antagonism to effective component It rings, is conducive to the performance of fertilizer fertilizer.
6th, yeast selenium foliar fertilizer of the present invention can promote crop growth, and increase the disease resistance of crops.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present application, constitutes part of this application, this Shen Illustrative embodiments and their description please are not constituted an undue limitation on the present application for explaining the application.In the accompanying drawings:
Fig. 1 is yeast selenium blade face fertilizer suspending agent production flow diagram in the embodiment of the present invention 1;
Fig. 2 is that yeast selenium foliar fertilizer can wet-milling production flow diagram in the embodiment of the present invention 7.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description.It should be noted that described embodiment only actually is a part of the embodiment of the present invention, rather than whole realities Example is applied, and is actually merely illustrative, never as to the present invention and its application or any restrictions used.The guarantor of the application Protect range as defined by the appended claims.
Embodiment 1:
Herein described yeast selenium foliar fertilizer, production method and the specific embodiment of application, the raw material in this method include Following parts by weight, wherein soluble 10 parts of Se-enriched yeast, 1 part of synergist, 1 part of dispersing agent, 1 part of wetting agent, 0.1 part of defoaming agent, 1 part of antifreeze, 0.1 part of thickener, 85 parts of water.Wherein the antifreeze in the present embodiment is ethylene glycol, another reality of the invention Applying the antifreeze in example is one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures. Defoaming agent in the present embodiment is polyoxyethylene polyoxy propyl alcohol amidogen ether, and the defoaming agent in another embodiment of the invention is emulsification Silicone oil, higher alcohols fatty acid ester compounded object, polyoxyethylene polyoxy propyl alcohol amidogen ether, gather polyoxyethylene polyoxypropylene pentaerythrite ether One or more mixtures of oxypropylene glycerin ether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer.This implementation Synergist is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer, the increasing in another embodiment of the invention in example Effect agent is one or more mixing of phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound Object.Wetting agent is naphthalene sulfonate and anion compound in the present embodiment, and the wetting agent in another embodiment of the invention is Naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphoric acid One of ester or a variety of mixtures.Dispersing agent is inorganic salts in the present embodiment, the dispersion in another embodiment of the invention Agent is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.This implementation Thickener is polysaccharide compound in example.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Shown in Figure 1, yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25 DEG C in fermentation temperature, tank Pressure is 0.02Mpa, ventilatory capacity 500m320h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 25min is centrifuged in the case where revolving speed is 2500rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 100MPa and handle 20min, obtain the soluble Se-enriched yeast.
Soluble 10 parts of the Se-enriched yeast is taken, and 1 part of synergist is added thereto, 1 part of dispersing agent, 1 part of wetting agent, disappears 0.1 part of infusion, 1 part of antifreeze, 0.1 part of thickener, 85 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5, obtains yeast selenium blade face fertilizer suspending agent.
The present embodiment application include:
Yeast selenium blade face fertilizer suspending agent is applicable in the work such as various grains, melon fruits and vegetables, medicinal material, tealeaves, tobacco in the present embodiment Object (such as: rice, strawberry, lichee, longan, banana, apple, jujube, grape, peach, pears, watermelon, tealeaves, vegetables, medicinal material, rape, Garlic, potato, soybean).
Yeast selenium in the present embodiment is produced during fermentating breeding to the effective metabolite of crops, is such as grown Element, multivitamin and amino acid etc. can stimulate and adjust crop growth, improve increment.And farming can be induced Peroxidase, polyphenol oxidase etc. participate in the defense reaction of crops in object, are conducive to the diseases prevention, disease-resistant of flowers and trees.
The spray method of yeast selenium blade face fertilizer suspending agent in the present embodiment are as follows:
First the yeast selenium blade face fertilizer suspending agent obtained in the present embodiment is diluted with water, then with dilution after resulting suspending agent Remove dilution pesticide;
By after dilution selenium-enriched foliage fertilizer and pesticide be fitted into sprinkling tank in, sprayed with unmanned plane;Or carry out manually or Electronic sprinkling;
Yeast selenium blade face fertilizer suspending agent can be sprayed in farming early growth period and growth medium;
, can be by spray head against based on the leaf back side to improve Foliage Absorption, uniformly sprinkling one time, not beat repeatedly back and forth;
If it is either progress at dusk before preferably in the morning 10 points of the outdoor outdoor cultivation of spring and autumn three quarters, such energy Avoid the high temperature period, reduce evaporation, if winter booth vegetable melon and fruit plantation etc. can period at noon, because of winter temperature It is low, and temperature of shed highest between 10 AM at 4 points in afternoon, it is most active that plant growth absorbs nutrient.
The yeast selenium foliar fertilizer of the present embodiment by the way that inorganic selenium is converted to organic selenium, have small toxicity, environmental pollution it is small, The advantages that bioactivity height and easily absorption, and the toxicity of yeast selenium is also far below chelating selenium, the food nonhazardous containing yeast selenium, Safety is free from side effects;Se content in yeast selenium is high, and cooperation synergist keeps the fertilizer efficiency of yeast selenium foliar fertilizer in the present embodiment long, Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs selenium element, improves the Se content of crops;It can drop Heavy metal in crops is solved, increases the disease resistance of crops, crop yield is made to increase;Promote blade face by the way that synergist is added The adhesion strength of solution drop, greatly prevents foliar fertilizer drop because environmental factor is low from blade face, evaporates, greatly reduce fertilizer The waste of material;By the way that synergist is added, it is possible to reduce hard water ions generate the adverse effect such as antagonism to effective component, are conducive to fertilizer Expect the performance of fertilizer.
Embodiment 2:
Herein described yeast selenium foliar fertilizer, production method and another specific embodiment of application, in the embodiment Raw material includes following parts by weight, wherein soluble 35 parts of Se-enriched yeast, 10 parts of synergist, 15 parts of dispersing agent, 15 parts of wetting agent, disappearing 0.5 part of infusion, 5 parts of antifreeze, 1.5 parts of thickener, 20 parts of water.Wherein the antifreeze in the present embodiment is ethyl alcohol, of the invention Antifreeze in another embodiment is one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or more Kind mixture.Defoaming agent in the present embodiment is polypropylene glycerol aether, and the defoaming agent in another embodiment of the invention is The fatty acid ester compounded object of silicone emulsion, higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy Propanolamine Ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer one or more mixtures.This Synergist is polyoxyethylene ether compound in embodiment, the synergist in another embodiment of the invention be phosphoric acid hydrogen dimethylamino, One or more mixtures of citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.It is soaked in the present embodiment Agent is alkyl sulfate, and the wetting agent in another embodiment of the invention is naphthalene sulfonate and anion compound, alkyl sulfide One of hydrochlorate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.This implementation Dispersing agent is polycarboxylate in example, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate, phosphate, One of nonionic or comb-type structure anion or a variety of mixtures.Thickener is polysaccharide compound in the present embodiment.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 30 DEG C in fermentation temperature, tank Pressure is 0.05Mpa, ventilatory capacity 600m325h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3000rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 150MPa and handle 25min, obtain the soluble Se-enriched yeast.
Soluble 35 parts of the Se-enriched yeast is taken, and 10 parts of synergist, 15 parts of dispersing agent, wetting agent 15 are added thereto Part, 0.5 part of defoaming agent, 5 parts of antifreeze, 1.5 parts of thickener, 20 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.8, obtains yeast selenium blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 3
Herein described yeast selenium foliar fertilizer, production method and practical one embodiment of application, in the embodiment Raw material includes following parts by weight, wherein soluble 60 parts of Se-enriched yeast, 20 parts of synergist, 30 parts of dispersing agent, 30 parts of wetting agent, disappearing 1 part of infusion, 10 parts of antifreeze, 3 parts of thickener, 50 parts of water.Wherein the antifreeze in the present embodiment is calcium chloride, and of the invention is another Antifreeze in one embodiment is one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of Mixture.Defoaming agent in the present embodiment is silicone emulsion, the defoaming agent in another embodiment of the invention be silicone emulsion, The fatty acid ester compounded object of higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcohol amidogen ether, polyoxypropylene One or more mixtures of glycerin ether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer.Increase in the present embodiment Effect agent is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer, and the synergist in another embodiment of the invention is One or more mixtures of phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.This Wetting agent is sulfonate in embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing Object.Dispersing agent is phosphate in the present embodiment, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate, One of phosphate, nonionic or comb-type structure anion or a variety of mixtures.Thickener is multiple for polysaccharide in the present embodiment Close object.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 35 DEG C in fermentation temperature, tank Pressure is 0.07Mpa, ventilatory capacity 700m330h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 35min is centrifuged in the case where revolving speed is 3500rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 200MPa and handle 30min, obtain the soluble Se-enriched yeast.
Take soluble 60 parts of the Se-enriched yeast, and be added thereto 20 parts of synergist, 30 parts of dispersing agent, 1 part of defoaming agent, 10 parts of antifreeze, 30 parts of wetting agent, 3 parts of thickener, 50 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 7.2, obtains yeast selenium blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 4
Herein described yeast selenium foliar fertilizer, production method and another practical embodiment of application, in the embodiment Raw material include following parts by weight, wherein soluble 20 parts of Se-enriched yeast, 5 parts of synergist, 10 parts of dispersing agent, 10 parts of wetting agent, 0.2 part of defoaming agent, 3 parts of antifreeze, 0.6 part of thickener, 45 parts of water.Wherein the antifreeze in the present embodiment is methanol, the present invention Another embodiment in antifreeze be one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or A variety of mixtures.Defoaming agent in the present embodiment is the fatty acid ester compounded object of higher alcohols, in another embodiment of the invention Defoaming agent is silicone emulsion, the fatty acid ester compounded object of higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy Propyl alcohol amidogen ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene glycerin ether or the one or more of dimethyl silicone polymer mix Close object.Synergist is polyoxyethylene ether compound in the present embodiment, and the synergist in another embodiment of the invention is phosphoric acid One or more mixtures of hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.This implementation Wetting agent is fatty acid in example, and the wetting agent in another embodiment of the invention is naphthalene sulfonate and anion compound, alkane One of base sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.This Dispersing agent is nonionic in embodiment, and the dispersing agent in another embodiment of the invention is inorganic salts, polycarboxylate, phosphoric acid One of ester, nonionic or comb-type structure anion or a variety of mixtures.Thickener in the present embodiment is that polysaccharide is compound Object.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 26 DEG C in fermentation temperature, tank Pressure is 0.03Mpa, ventilatory capacity 550m322h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 27min is centrifuged in the case where revolving speed is 2700rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 120MPa and handle 22min, obtain the soluble Se-enriched yeast.
Soluble 20 parts of the Se-enriched yeast is taken, and 5 parts of synergist, 10 parts of dispersing agent, defoaming agent 0.2 are added thereto Part, 3 parts of antifreeze, 10 parts of wetting agent, 0.6 part of thickener, 45 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.6, obtains yeast selenium blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 5
The application suspending agent one embodiment, the raw material in the embodiment includes following parts by weight, wherein soluble selenium-rich 50 parts of yeast, 15 parts of synergist, 25 parts of dispersing agent, 25 parts of wetting agent, 0.8 part of defoaming agent, 8 parts of antifreeze, 1.8 parts of thickener, 30 parts of water.Wherein the antifreeze in the present embodiment is salt water, and the antifreeze in another embodiment of the invention is ethylene glycol, first One of alcohol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures.Defoaming agent in the present embodiment is poly- two Methylsiloxane, the defoaming agent in another embodiment of the invention are silicone emulsion, the fatty acid ester compounded object of higher alcohols, polyoxy Ethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcohol amidogen ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene are sweet One or more mixtures of oily ether or dimethyl silicone polymer.Synergist is phosphoric acid hydrogen dimethylamino, citric acid three in the present embodiment First and tamarind gum polymer, the synergist in another embodiment of the invention are phosphoric acid hydrogen dimethylamino, citric acid front three and sieve One or more mixtures of shop sign in the form of a streamer xanthan polymer or polyoxyethylene ether compound.Wetting agent is aliphatic ester sulphur in the present embodiment Hydrochlorate, the wetting agent in another embodiment of the invention are naphthalene sulfonate and anion compound, alkyl sulfate, sulfonic acid One of salt, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.Disperse in the present embodiment Agent is comb-type structure anion, and the dispersing agent in another embodiment of the invention is inorganic salts, polycarboxylate, phosphate, non- One of ion or comb-type structure anion or a variety of mixtures.Thickener in the present embodiment is polysaccharide compound.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 33 DEG C in fermentation temperature, tank Pressure is 0.06Mpa, ventilatory capacity 650m328h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 33min is centrifuged in the case where revolving speed is 3300rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 180MPa and handle 28min, obtain the soluble Se-enriched yeast.
Soluble 50 parts of the Se-enriched yeast is taken, and 15 parts of synergist, 25 parts of dispersing agent, defoaming agent 0.8 are added thereto Part, 8 parts of antifreeze, 25 parts of wetting agent, 1.8 parts of thickener, 30 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 7.0, obtains yeast selenium blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 6
Another embodiment of the application suspending agent, the raw material in the embodiment includes following parts by weight, wherein soluble rich 30 parts of selenium yeast, 12 parts of synergist, 17 parts of dispersing agent, 17 parts of wetting agent, 0.6 part of defoaming agent, 6 parts of antifreeze, thickener 1.2 Part, 20 parts of water.Wherein the antifreeze in the present embodiment is water-soluble amide, and the antifreeze in another embodiment of the invention is One of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures.Defoaming in the present embodiment Agent is the fatty acid ester compounded object of higher alcohols, and the defoaming agent in another embodiment of the invention is silicone emulsion, higher alcohols fat Acid esters compound, polyoxyethylene polyoxy propyl alcohol amidogen ether, polypropylene glycerol aether, gathers polyoxyethylene polyoxypropylene pentaerythrite ether One or more mixtures of oxypropylene polyoxyethylene glycerol ether or dimethyl silicone polymer.Synergist is phosphoric acid in the present embodiment The mixture of hydrogen dimethylamino, citric acid front three and tamarind gum polymer and polyoxyethylene ether compound, another reality of the invention Apply one kind that the synergist in example is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound Or a variety of mixtures.Wetting agent is carboxylic acid soaps in the present embodiment, and the wetting agent in another embodiment of the invention is naphthalene sulphur In hydrochlorate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate One or more mixtures.Dispersing agent is inorganic salts and polycarboxylate mixture, another reality of the invention in the present embodiment It is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixed for applying dispersing agent in example Close object.Thickener in the present embodiment is polysaccharide compound.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium blade face fertilizer suspending agent making step is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 29 DEG C in fermentation temperature, tank Pressure is 0.04Mpa, ventilatory capacity 590m3It is cultivated under conditions of/h for 24 hours, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 29min is centrifuged in the case where revolving speed is 2900rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 140MPa and handle 24min, obtain the soluble Se-enriched yeast.
Soluble 30 parts of the Se-enriched yeast is taken, and 12 parts of synergist, 17 parts of dispersing agent, defoaming agent 0.6 are added thereto Part, 6 parts of antifreeze, 17 parts of wetting agent, 1.2 parts of thickener, 20 parts of water are uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.9, obtains yeast selenium blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 7
Herein described yeast selenium foliar fertilizer, one of production method and application can wet-milling embodiment, in the embodiment Raw material includes following parts by weight, wherein 10 parts of soluble Se-enriched yeast, 1 part of wetting agent, 1 part of dispersing agent, 88 parts of anhydrous sodium sulphate.Its Middle anhydrous sodium sulphate is sodium sulphate.Wetting agent is naphthalene sulfonate and anion compound, another implementation of the invention in the present embodiment Wetting agent in example be naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, One of carboxylic acid soaps or phosphate or a variety of mixtures.Dispersing agent is inorganic salts, of the invention another in the present embodiment Dispersing agent in embodiment is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of Mixture.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
It is shown in Figure 2, yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25 DEG C in fermentation temperature, tank Pressure is 0.02Mpa, ventilatory capacity 500m320h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 25min is centrifuged in the case where revolving speed is 2500rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 100MPa and handle 20min, obtain the soluble Se-enriched yeast.
Soluble 10 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
1 part of wetting agent, 1 part of dispersing agent, 88 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Level-one mixing is carried out, primary mixes are obtained;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
The present embodiment application include:
In the present embodiment yeast selenium foliar fertilizer can wet-milling be applicable in various grains, melon fruits and vegetables, medicinal material, tealeaves, tobacco etc. work Object (such as: rice, strawberry, lichee, longan, banana, apple, jujube, grape, peach, pears, watermelon, tealeaves, vegetables, medicinal material, rape, Garlic, potato, soybean).
Yeast selenium in the present embodiment is produced during fermentating breeding to the effective metabolite of crops, is such as grown Element, multivitamin and amino acid etc. can stimulate and adjust crop growth, improve increment.And farming can be induced Peroxidase, polyphenol oxidase etc. participate in the defense reaction of crops in object, are conducive to the diseases prevention, disease-resistant of flowers and trees.
In the present embodiment yeast selenium foliar fertilizer can wet-milling application method are as follows:
First by the yeast selenium foliar fertilizer obtained in the present embodiment can wet-milling be diluted with water, then with dilution after resulting suspending agent Remove dilution pesticide;
By after dilution selenium-enriched foliage fertilizer and pesticide be fitted into sprinkling tank in, sprayed with unmanned plane;Or carry out manually or Electronic sprinkling;
Yeast selenium blade face fertilizer suspending agent can be sprayed in farming early growth period and growth medium;
, can be by spray head against based on the leaf back side to improve Foliage Absorption, uniformly sprinkling one time, not beat repeatedly back and forth;
If it is either progress at dusk before preferably in the morning 10 points of the outdoor outdoor cultivation of spring and autumn three quarters, such energy Avoid the high temperature period, reduce evaporation, if winter booth vegetable melon and fruit plantation etc. can period at noon, because of winter temperature It is low, and temperature of shed highest between 10 AM at 4 points in afternoon, it is most active that plant growth absorbs nutrient.
The yeast selenium foliar fertilizer of the present embodiment by the way that inorganic selenium is converted to organic selenium, have small toxicity, environmental pollution it is small, The advantages that bioactivity height and easily absorption, and the toxicity of yeast selenium is also far below chelating selenium, the food nonhazardous containing yeast selenium, Safety is free from side effects;Se content in yeast selenium is high, and cooperation synergist keeps the fertilizer efficiency of yeast selenium foliar fertilizer in the present embodiment long, Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs selenium element, improves the Se content of crops;It can drop Heavy metal in crops is solved, increases the disease resistance of crops, crop yield is made to increase;Promote blade face by the way that synergist is added The adhesion strength of solution drop, greatly prevents foliar fertilizer drop because environmental factor is low from blade face, evaporates, greatly reduce The waste of fertilizer;By the way that synergist is added, it is possible to reduce hard water ions generate the adverse effect such as antagonism to effective component, are conducive to The performance of fertilizer fertilizer.
Embodiment 8
Herein described yeast selenium foliar fertilizer, production method and application another can wet-milling embodiment, in the embodiment Raw material include following parts by weight, wherein 60 parts of soluble Se-enriched yeast, 30 parts of wetting agent, 30 parts of dispersing agent, anhydrous sodium sulphate 30 Part.Wherein anhydrous sodium sulphate is sodium sulphate.Wetting agent is alkyl sulfate in the present embodiment, the profit in another embodiment of the invention Humectant is naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps Or one of phosphate or a variety of mixtures.Dispersing agent is polycarboxylate, another embodiment of the invention in the present embodiment In dispersing agent be one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixing Object.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 35 DEG C in fermentation temperature, tank Pressure is 0.07Mpa, ventilatory capacity 700m330h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 35min is centrifuged in the case where revolving speed is 3500rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 200MPa and handle 30min, obtain the soluble Se-enriched yeast.
Soluble 60 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
30 parts of wetting agent, 30 parts of dispersing agent, 30 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Middle progress level-one mixing, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 9
The application yeast selenium foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight, In, 35 parts of soluble Se-enriched yeast, 15 parts of wetting agent, 15 parts of dispersing agent, 35 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This Wetting agent is sulfonate in embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing Object.Dispersing agent is phosphate in the present embodiment, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate, One of phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 30 DEG C in fermentation temperature, tank Pressure is 0.05Mpa, ventilatory capacity 600m325h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3000rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 150MPa and handle 25min, obtain the soluble Se-enriched yeast.
Soluble 35 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
15 parts of wetting agent, 15 parts of dispersing agent, 35 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Middle progress level-one mixing, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 10
The application yeast selenium foliar fertilizer another can wet-milling embodiment, the raw material in the embodiment includes following parts by weight, Wherein, 20 parts of soluble Se-enriched yeast, 10 parts of wetting agent, 10 parts of dispersing agent, 60 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate. Wetting agent is fatty acid in the present embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing Object.Dispersing agent is nonionic in the present embodiment, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate, One of phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 26 DEG C in fermentation temperature, tank Pressure is 0.03Mpa, ventilatory capacity 55000m322h is cultivated under conditions of/h, obtains fermentation liquid;Contain in the fermentation medium 2% glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 26min is centrifuged in the case where revolving speed is 2600rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 120MPa and handle 22min, obtain the soluble Se-enriched yeast.
Soluble 20 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
10 parts of wetting agent, 10 parts of dispersing agent, 60 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Middle progress level-one mixing, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 11
The application yeast selenium foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight, In, 50 parts of soluble Se-enriched yeast, 25 parts of wetting agent, 25 parts of dispersing agent, 50 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This Wetting agent is aliphatic ester sulfate in embodiment, the wetting agent in another embodiment of the invention be naphthalene sulfonate and yin from One of sub- compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate are more Kind mixture.Dispersing agent is comb-type structure anion in the present embodiment, and the dispersing agent in another embodiment of the invention is nothing One of machine salt, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 33 DEG C in fermentation temperature, tank Pressure is 0.06Mpa, ventilatory capacity 600m328h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3300rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 180MPa and handle 28min, obtain the soluble Se-enriched yeast.
Soluble 50 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
25 parts of wetting agent, 25 parts of dispersing agent, 50 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Middle progress level-one mixing, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 12
The application yeast selenium foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight, In, 36 parts of soluble Se-enriched yeast, 16 parts of wetting agent, 16 parts of dispersing agent, 32 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This Wetting agent is carboxylic acid soaps in embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing Object.Dispersing agent is the mixture of inorganic salts and polycarboxylate in the present embodiment, the dispersing agent in another embodiment of the invention For one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Se-enriched yeast derives from Shandong Bora benefit bioengineering limited liability company, is obtained using the fermentation of Ruan's Candida ?;Ruan's Candida (Candida utilis) CUM was preserved in China typical culture collection on April 19th, 2010 The heart, deposit number are CCTCC M 2010090.
Yeast selenium foliar fertilizer can wet-milling making step it is as follows:
Three-level activates Ruan's Candida CCTCC M 2010090, adds Selenium Sulphate point in the medium in activation process It is not 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid;
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 31 DEG C in fermentation temperature, tank Pressure is 0.04Mpa, ventilatory capacity 620m326h is cultivated under conditions of/h, obtains fermentation liquid;Contain 2% in the fermentation medium Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
The fermentation liquid 31min is centrifuged in the case where revolving speed is 3100rpm and is cleaned with clear water, is so repeated 3 times, by being centrifuged It must be deposited under 160MPa and handle 26min, obtain the soluble Se-enriched yeast.
Soluble 36 parts of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain selenium-rich ferment after air-drying It is female;
16 parts of wetting agent, 16 parts of dispersing agent, 32 parts of anhydrous sodium sulphate are put into raw material mixing machine with Se-enriched yeast after described air-dry Middle progress level-one mixing, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into The mixing of row second level, obtaining the yeast selenium foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
As can be seen from the above embodiments beneficial effect existing for the application is:
The first, yeast selenium foliar fertilizer of the invention has small toxicity, environmental pollution by the way that inorganic selenium is converted to organic selenium The advantages that small, bioactivity is high and easy absorption, and the toxicity of yeast selenium is also far below chelating selenium, and the food containing yeast selenium is nontoxic Evil, is free from side effects safely.
The second, the Se content in yeast selenium of the present invention is high, and cooperation synergist keeps the fertilizer efficiency of yeast selenium foliar fertilizer of the present invention long, Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs selenium element, improves the Se content of crops.
Heavy metal in the degradable crops of Se-enriched yeast in third, the present invention, makes crop yield increase.
4th, the present invention promotes the adhesion strength of foliar fertilizer drop by the way that synergist is added, and greatly prevents blade face solution Drop evaporates because environmental factor is low from blade face, greatly reduces the waste of fertilizer.
5th, the present invention is by being added synergist, it is possible to reduce hard water ions generate the unfavorable shadows such as antagonism to effective component It rings, is conducive to the performance of fertilizer fertilizer.
6th, yeast selenium foliar fertilizer of the present invention can promote crop growth, and increase the disease resistance of crops.
Although some specific embodiments of the invention are described in detail by example, the skill of this field Art personnel it should be understood that example above merely to being illustrated, the range being not intended to be limiting of the invention.Although referring to before Stating embodiment, invention is explained in detail, for those skilled in the art, still can be to aforementioned reality Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features.It is all of the invention Within spirit and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention. The scope of the present invention is defined by the appended claims.

Claims (10)

1. a kind of yeast selenium foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Se-enriched yeast 10- 60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, 1-20 parts of synergist, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, thickener 0.1-3 parts, surplus be water.
2. yeast selenium foliar fertilizer according to claim 1, which is characterized in that the raw material including following parts by weight, wherein can 20-50 parts of dissolubility Se-enriched yeast, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, 5-15 parts of synergist, 0.3-0.8 parts of defoaming agent, 3-8 parts of antifreeze, 1-2 parts of thickener, surplus are water.
3. a kind of yeast selenium foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Se-enriched yeast 10- 60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.
4. yeast selenium foliar fertilizer according to claim 3, which is characterized in that the raw material including following parts by weight, wherein can 20-50 parts of dissolubility Se-enriched yeast, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, surplus are anhydrous sodium sulphate.
5. yeast selenium foliar fertilizer according to claim 1 to 2, which is characterized in that the synergist include phosphoric acid hydrogen dimethylamino, One or more mixtures of citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.
6. yeast selenium foliar fertilizer described in -4 according to claim 1, which is characterized in that
The wetting agent includes: naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulphur One of hydrochlorate, carboxylic acid soaps or phosphate or a variety of mixtures;
The dispersing agent includes: one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or more Kind mixture;
The thickener includes: polysaccharide compound.
7. one kind yeast selenium foliar fertilizer production method as described in claim 1 to 6, which is characterized in that soluble rich including production The step of selenium yeast:
Three-level activates Ruan's Candida, and adding Selenium Sulphate in the medium in activation process is respectively 0.1mg/ to ultimate density ML, 0.5mg/mL, 1mg/mL obtain seed culture fluid;
According to 1-3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25-35 DEG C in fermentation temperature, tank Pressure is 0.02-0.07Mpa, ventilatory capacity 500-700m320-30h is cultivated under conditions of/h, obtains fermentation liquid;The fermentation training It supports and contains 2% glucose, 1%NH in base4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1% Na2HPO4, 0.1%MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4
It is centrifuged the fermentation liquid 25-35min in the case where revolving speed is 2500-3500rpm and is cleaned with clear water, so repeat 3 times, it will be from It is deposited under 100-200MPa obtained by the heart and handles 20-30min, obtain the soluble Se-enriched yeast.
8. yeast selenium foliar fertilizer production method according to claim 7, which is characterized in that further include production yeast selenium blade face The step of fertilizer suspending agent:
Soluble 10-60 parts of the Se-enriched yeast is taken, and synergist 1-20 parts, 1-30 parts of wetting agent, dispersing agent are added thereto 1-30 parts, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus be water, be uniformly mixed;
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5-7.2, obtains yeast selenium blade face fertilizer suspending agent.
9. yeast selenium foliar fertilizer production method according to claim 7, which is characterized in that further include production yeast selenium blade face Fertilizer can wet-milling the step of:
Soluble 10-60 part of the Se-enriched yeast is taken, is placed under sterile ventilation condition and carries out air-dried, obtain air-drying selenium-rich ferment afterwards It is female;
1-30 parts of wetting agents, 1-30 parts of dispersing agents, anhydrous sodium sulphate and the air-dried rear Se-enriched yeast are mixed, mixture is put into original Expect to carry out level-one mixing in mixing machine, obtains primary mixes;
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material;
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained;
The available the second-order separation object separated in bag filter and the level-one isolate are sent into mixing machine and carry out two Grade mixing, obtaining the yeast selenium foliar fertilizer can wet-milling.
10. a kind of application of the yeast selenium foliar fertilizer as described in claim 1 to 6.
CN201811377646.6A 2018-11-19 2018-11-19 Yeast selenium foliar fertilizer, production method and application Pending CN109232107A (en)

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CN110431975A (en) * 2019-08-23 2019-11-12 长汀县绿友生态农业专业合作社 A kind of high-yield planting method of selenium-rich lotus seeds
CN112552118A (en) * 2021-01-04 2021-03-26 方连明 Selenium-rich foliar fertilizer and preparation method and application thereof
CN112745157A (en) * 2020-10-30 2021-05-04 广西壮族自治区农业科学院 Selenium-enriched biological foliar fertilizer and preparation method and application thereof

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