CN109438039A - Yeast zinc foliar fertilizer, production method and application - Google Patents
Yeast zinc foliar fertilizer, production method and application Download PDFInfo
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- CN109438039A CN109438039A CN201811378740.3A CN201811378740A CN109438039A CN 109438039 A CN109438039 A CN 109438039A CN 201811378740 A CN201811378740 A CN 201811378740A CN 109438039 A CN109438039 A CN 109438039A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C12N1/16—Yeasts; Culture media therefor
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Abstract
This application discloses a kind of yeast zinc foliar fertilizer, production method and applications, yeast zinc blade face fertilizer suspending agent includes the raw material of following parts by weight: soluble Zinc-rich saccharomyces cerevisiae 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, and 1-20 parts of synergist, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus are water；Yeast zinc foliar fertilizer can wet-milling include following parts by weight raw material: soluble Zinc-rich saccharomyces cerevisiae 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.Yeast zinc foliar fertilizer cooperation synergist of the invention can make fertilizer efficiency long, promote the adhesion strength of foliar fertilizer drop；Zinc-rich saccharomyces cerevisiae can enhance the rhizome disease resistance of crops；Hard water ions can be reduced, the adverse effect such as antagonism is generated to effective component；The yield and percentage of fertile fruit of crops can be improved；Improve the quality of crops；Improve the resistance of crops.
Description
Technical field
Present invention crop technical field of fertilizers concerning farmers, specifically, more particularly to a kind of yeast zinc foliar fertilizer, production method
And application.
Background technique
For zinc primarily as the metal activation agent of enzyme in plant, the zinc-containing metal enzyme found earliest is in carbonic anhydride
Enzyme, this enzyme are distributed very extensively in plant, are primarily present in chloroplaset, it is catalyzed the aquation of carbon dioxide, promote
The fixation of carbon dioxide in photosynthesis, zinc-deficiency is that the activity of carbonic anhydrase reduces, so zinc plays an important role to plant.Zinc exists
Also participate in the synthesis of auxin in plant, when zinc-deficiency, the intracorporal growth cellulose content of plant can be reduced, and growth and development is stagnated
It is packed into, stipes shortens, and plant is short and small, and leaf expansion elongation receives retardance, forms leaflet, and be in leafage shape, occurs between vein light
Green, yellow or white rust staining, especially in old leaf.It is that plant height is uneven in variable rate technology, the maturity period postpones, fruit development
It is bad.The plant of the reported zinc-deficiency in China has " shrunk rice seedling " of rice, " runt seedling ", " white bud " of corn, the fruit trees such as citrus
" little leaf " etc..Crops can be made to absorb enough Zn-ef ficiencies by imposing zinc fertilizer to crops, promoted in crops
The aquation of carbon dioxide promotes nitrogen metabolism effect, improves the chlorosis phenomenon of plant to improve photosynthesis.Zinc is also
Play the role of enhancing stress resistance of plant.The disease resistance of crops can be improved in zinc.Zinc-rich fertilizer currently on the market is mainly
Zinc ore powder is mixed with conventional chemical fertilizers, since natural Zn-ef ficiency is with the presence of inorganic compound state, is not easy to plant absorption and is turned
Change.Extra zinc fertilizer, which is left in soil, will cause environmental pollution.Food containing yeast zinc is relative to the food containing chemical zinc and chelated zinc
Product, have the characteristics that nontoxicity, and food is safer.
It selects foliar fertilizer to have many benefits, can be used together with insecticide with fungicide, be that pretty economical is effective
Operating method；Leaf portion nutrition is supplemented using foliar fertilizer, high financial profit saves fertilizer；Because it is effective to directly feed flowers and trees
Nutrient solves soil and fixes to the absorption of nutrient；The operating of crops Nutrient Absorption is fast, and utilization rate is high, can meet needs in time；
Use environment requires not tight.But existing foliar fertilizer, because after the cuticula of blade face, Foliage Absorption nutrient penetrance is low.And it easily flows
It loses, during spraying, if misoperation or chance strong wind weather, easily low from blade face, wastes medical fluid；The chance easy leaching loss of rain, one
As in the case of, 4 hours chance rain need to apply fertilizer again after spray；If weather overdrying, medical fluid is dried rapidly image on blade face and is absorbed,
And then fertilizer efficiency.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of yeast zinc foliar fertilizers, which is characterized in that including following parts by weight
Raw material, wherein soluble Zinc-rich saccharomyces cerevisiae 10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, 1-20 parts of synergist, defoaming
0.1-1 parts of agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus are water.
Preferably, the raw material including following parts by weight, wherein soluble Zinc-rich saccharomyces cerevisiae 20-50 parts, 10-20 parts of wetting agent,
10-20 parts of dispersing agent, 5-15 parts of synergist, 0.3-0.8 parts of defoaming agent, 3-8 parts of antifreeze, 1-2 parts of thickener, surplus are water.
A kind of yeast zinc foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Zinc-rich saccharomyces cerevisiae
10-60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.
Preferably, the raw material including following parts by weight, wherein soluble Zinc-rich saccharomyces cerevisiae 20-50 parts, 10-20 parts of wetting agent,
10-20 parts of dispersing agent, surplus are anhydrous sodium sulphate.
Preferably, the synergist includes phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene
One or more mixtures of ether compound.
Preferably, the wetting agent includes: naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fat
One of acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures；
The dispersing agent includes: one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion
Or a variety of mixtures；
The thickener includes: polysaccharide compound.
Yeast zinc foliar fertilizer production method, which is characterized in that include the steps that making soluble Zinc-rich saccharomyces cerevisiae:
Three-level activates candida utili, and add zinc sulfate in the medium in activation process is to ultimate density respectively
0.1mg/mL, 0.5mg/mL, 1mg/mL obtain seed culture fluid；
According to 1-3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25-35 in fermentation temperature
DEG C, tank pressure is 0.02-0.07Mpa, ventilatory capacity 500-700m320-30h is cultivated under conditions of/h, obtains fermentation liquid；It is described
Contain 2% glucose, 1%NH in fermentation medium4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4、
0.1%Na2HPO4, 0.1% MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 25-35min is centrifuged in the case where revolving speed is 2500-3500rpm and is cleaned with clear water, is so repeated 3 times,
Centrifugation gained is deposited under 100-200MPa and handles 20-30min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Preferably, further include the steps that making yeast zinc blade face fertilizer suspending agent:
Soluble 10-60 parts of the Zinc-rich saccharomyces cerevisiae is taken, and synergist 1-20 parts is added thereto, 1-30 parts of wetting agent, divides
1-30 parts of powder, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 3 parts of thickener 0.1-, surplus are water, are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5-7.2, obtains the yeast zinc foliar fertilizer and suspends
Agent.
Preferably, further including the steps that making yeast zinc foliar fertilizer can wet-milling:
Soluble 10-60 part of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain air-drying zinc-rich afterwards
Yeast；
1-30 parts of wetting agents, 1-30 parts of dispersing agents, anhydrous sodium sulphate and the air-dried rear Zinc-rich saccharomyces cerevisiae are mixed, mixture is put
Enter progress level-one mixing in raw material mixing machine, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
The application of yeast zinc foliar fertilizer.
Compared with prior art, yeast zinc foliar fertilizer, production method and application provided by the invention reach following beneficial to effect
Fruit:
The first, inorganic zinc by being converted to organic zinc by yeast zinc foliar fertilizer of the invention, with bioactivity height and easily
The advantages that absorption, the food containing yeast zinc have the characteristics that nontoxicity relative to the food containing chemical zinc and chelated zinc, and food is more pacified
Entirely.
The second, the Zn content in yeast zinc of the present invention is high, and cooperation synergist keeps the fertilizer efficiency of yeast zinc foliar fertilizer of the present invention long,
Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs Zn-ef ficiency, improves the Zn content of crops.
Zinc-rich saccharomyces cerevisiae in third, the present invention can promote crop root part stalwartness, reduce root infection disease, enhance farming
The rhizome disease resistance of object.
4th, the present invention promotes the adhesion strength of foliar fertilizer drop by the way that synergist is added, and greatly prevents blade face solution
Drop evaporates because environmental factor is low from blade face, greatly reduces the waste of fertilizer.
5th, the present invention is by being added synergist, it is possible to reduce hard water ions generate the unfavorable shadows such as antagonism to effective component
It rings, is conducive to the performance of fertilizer fertilizer.
6th, yeast zinc foliar fertilizer of the present invention can promote crop growth, improve the yield and percentage of fertile fruit of crops.
7th, yeast zinc foliar fertilizer of the present invention can participate in the metabolism of protein in crops, so as to improve farming
The quality of object.
8th, yeast zinc foliar fertilizer of the present invention can greatly improve the resistance of crops.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present application, constitutes part of this application, this Shen
Illustrative embodiments and their description please are not constituted an undue limitation on the present application for explaining the application.In the accompanying drawings:
Fig. 1 is yeast zinc blade face fertilizer suspending agent production flow diagram in the embodiment of the present invention 1；
Fig. 2 is that yeast zinc foliar fertilizer can wet-milling production flow diagram in the embodiment of the present invention 7.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description.It should be noted that described embodiment only actually is a part of the embodiment of the present invention, rather than whole realities
Example is applied, and is actually merely illustrative, never as to the present invention and its application or any restrictions used.The guarantor of the application
Protect range as defined by the appended claims.
Embodiment 1:
Herein described yeast zinc foliar fertilizer, production method and the specific embodiment of application, the raw material in this method include
Following parts by weight, wherein soluble 10 parts of Zinc-rich saccharomyces cerevisiae, 1 part of synergist, 1 part of dispersing agent, 1 part of wetting agent, 0.1 part of defoaming agent,
1 part of antifreeze, 0.1 part of thickener, 85 parts of water.Wherein the antifreeze in the present embodiment is ethylene glycol, another reality of the invention
Applying the antifreeze in example is one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures.
Defoaming agent in the present embodiment is polyoxyethylene polyoxy propyl alcohol amidogen ether, and the defoaming agent in another embodiment of the invention is emulsification
Silicone oil, higher alcohols fatty acid ester compounded object, polyoxyethylene polyoxy propyl alcohol amidogen ether, gather polyoxyethylene polyoxypropylene pentaerythrite ether
One or more mixtures of oxypropylene glycerin ether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer.This implementation
Synergist is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer, the increasing in another embodiment of the invention in example
Effect agent is one or more mixing of phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound
Object.Wetting agent is naphthalene sulfonate and anion compound in the present embodiment, and the wetting agent in another embodiment of the invention is
Naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphoric acid
One of ester or a variety of mixtures.Dispersing agent is inorganic salts in the present embodiment, the dispersion in another embodiment of the invention
Agent is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.This implementation
Thickener is polysaccharide compound in example.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Shown in Figure 1, yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25 DEG C in fermentation temperature, tank
Pressure is 0.02Mpa, ventilatory capacity 500m320h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 25min is centrifuged in the case where revolving speed is 2500rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 100MPa, handles 20min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 10 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 1 part of synergist is added thereto, 1 part of dispersing agent, 1 part of wetting agent, disappears
0.1 part of infusion, 1 part of antifreeze, 0.1 part of thickener, 85 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5, obtains yeast zinc blade face fertilizer suspending agent.
The present embodiment application include:
Yeast zinc blade face fertilizer suspending agent is applicable in the work such as various grains, melon fruits and vegetables, medicinal material, tealeaves, tobacco in the present embodiment
Object (such as: rice, strawberry, lichee, longan, banana, apple, jujube, grape, peach, pears, watermelon, tealeaves, vegetables, medicinal material, rape,
Garlic, potato, soybean).
Yeast zinc in the present embodiment is produced during fermentating breeding to the effective metabolite of crops, is such as grown
Element, multivitamin and amino acid etc. can stimulate and adjust crop growth, improve increment.And farming can be induced
Peroxidase, polyphenol oxidase etc. participate in the defense reaction of crops in object, are conducive to the diseases prevention, disease-resistant of flowers and trees.
The spray method of yeast zinc blade face fertilizer suspending agent in the present embodiment are as follows:
The yeast zinc blade face fertilizer suspending agent obtained in the present embodiment is diluted with water；
Zinc-rich foliage fertilizer respectively after dilution is fitted into sprinkling tank, is sprayed with unmanned plane；Or it is manually or electrically sprayed
It spills；
Yeast zinc blade face fertilizer suspending agent can be sprayed in farming early growth period and growth medium；
, can be by spray head against based on the leaf back side to improve Foliage Absorption, uniformly sprinkling one time, not beat repeatedly back and forth；
If it is either progress at dusk before preferably in the morning 10 points of the outdoor outdoor cultivation of spring and autumn three quarters, such energy
Avoid the high temperature period, reduce evaporation, if winter booth vegetable melon and fruit plantation etc. can period at noon, because of winter temperature
It is low, and temperature of shed highest between 10 AM at 4 points in afternoon, it is most active that plant growth absorbs nutrient.
Yeast zinc foliar fertilizer of the invention has bioactivity height and easily absorption etc. by the way that inorganic zinc is converted to organic zinc
Advantage, the food containing yeast zinc have the characteristics that nontoxicity relative to the food containing chemical zinc and chelated zinc, and food is safer；This
Zn content in invention yeast zinc is high, and cooperation synergist keeps the fertilizer efficiency of yeast zinc foliar fertilizer of the present invention long, the wetting on leaf
Time is long, and penetrance is high, is easy to crops and absorbs Zn-ef ficiency, improves the Zn content of crops；It is strong to can promote crop root part
It is strong, root infection disease is reduced, the rhizome disease resistance of crops is enhanced；Promote the viscous of foliar fertilizer drop by the way that synergist is added
Attached power greatly prevents foliar fertilizer drop because environmental factor is low from blade face, evaporates, greatly reduce the wave of fertilizer
Take；Synergist is added, it is possible to reduce hard water ions generate the adverse effect such as antagonism to effective component, are conducive to the hair of fertilizer fertilizer
It waves；It can promote crop growth, improve the yield and percentage of fertile fruit of crops；The metabolism that can participate in protein in crops is made
With so as to improve the quality of crops；And the resistance of crops can be greatly improved.
Embodiment 2:
Herein described yeast zinc foliar fertilizer, production method and another specific embodiment of application, in the embodiment
Raw material includes following parts by weight, wherein soluble 35 parts of Zinc-rich saccharomyces cerevisiae, 10 parts of synergist, 15 parts of dispersing agent, 15 parts of wetting agent,
0.5 part of defoaming agent, 5 parts of antifreeze, 1.5 parts of thickener, 20 parts of water.Wherein the antifreeze in the present embodiment is ethyl alcohol, the present invention
Another embodiment in antifreeze be one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or
A variety of mixtures.Defoaming agent in the present embodiment is polypropylene glycerol aether, the defoaming agent in another embodiment of the invention
For silicone emulsion, the fatty acid ester compounded object of higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy Propanolamine
Ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer one or more mixtures.This
Synergist is polyoxyethylene ether compound in embodiment, the synergist in another embodiment of the invention be phosphoric acid hydrogen dimethylamino,
One or more mixtures of citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.It is soaked in the present embodiment
Agent is alkyl sulfate, and the wetting agent in another embodiment of the invention is naphthalene sulfonate and anion compound, alkyl sulfide
One of hydrochlorate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.This implementation
Dispersing agent is polycarboxylate in example, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate, phosphate,
One of nonionic or comb-type structure anion or a variety of mixtures.Thickener is polysaccharide compound in the present embodiment.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 30 DEG C in fermentation temperature, tank
Pressure is 0.05Mpa, ventilatory capacity 600m325h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3000rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 150MPa, handles 25min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 35 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 10 parts of synergist, 15 parts of dispersing agent, wetting agent 15 are added thereto
Part, 0.5 part of defoaming agent, 5 parts of antifreeze, 1.5 parts of thickener, 20 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.8, obtains yeast zinc blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 3
Herein described yeast zinc foliar fertilizer, production method and practical one embodiment of application, in the embodiment
Raw material includes following parts by weight, wherein soluble 60 parts of Zinc-rich saccharomyces cerevisiae, 20 parts of synergist, 30 parts of dispersing agent, 30 parts of wetting agent,
1 part of defoaming agent, 10 parts of antifreeze, 3 parts of thickener, 50 parts of water.Wherein the antifreeze in the present embodiment is calcium chloride, of the invention
Antifreeze in another embodiment is one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or more
Kind mixture.Defoaming agent in the present embodiment is silicone emulsion, and the defoaming agent in another embodiment of the invention is emulsification silicon
Oil, the fatty acid ester compounded object of higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcohol amidogen ether, polyoxy
One or more mixtures of propylene glycerin ether, polyoxyethylene polyoxypropylene glycerin ether or dimethyl silicone polymer.The present embodiment
Middle synergist is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer, the synergy in another embodiment of the invention
Agent is one or more mixing of phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound
Object.Wetting agent is sulfonate in the present embodiment, and the wetting agent in another embodiment of the invention is naphthalene sulfonate and anion
One of compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate are a variety of
Mixture.Dispersing agent is phosphate in the present embodiment, and the dispersing agent in another embodiment of the invention is inorganic salts, polycarboxylic acids
One of salt, phosphate, nonionic or comb-type structure anion or a variety of mixtures.Thickener is polysaccharide in the present embodiment
Compound.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 35 DEG C in fermentation temperature, tank
Pressure is 0.07Mpa, ventilatory capacity 700m330h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 35min is centrifuged in the case where revolving speed is 3500rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 200MPa, handles 30min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 60 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 20 parts of synergist, 30 parts of dispersing agent, defoaming agent 1 are added thereto
Part, 10 parts of antifreeze, 30 parts of wetting agent, 3 parts of thickener, 50 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 7.2, obtains yeast zinc blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 4
Herein described yeast zinc foliar fertilizer, production method and another practical embodiment of application, in the embodiment
Raw material include following parts by weight, wherein soluble 20 parts of Zinc-rich saccharomyces cerevisiae, 5 parts of synergist, 10 parts of dispersing agent, 10 parts of wetting agent,
0.2 part of defoaming agent, 3 parts of antifreeze, 0.6 part of thickener, 45 parts of water.Wherein the antifreeze in the present embodiment is methanol, the present invention
Another embodiment in antifreeze be one of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or
A variety of mixtures.Defoaming agent in the present embodiment is the fatty acid ester compounded object of higher alcohols, in another embodiment of the invention
Defoaming agent is silicone emulsion, the fatty acid ester compounded object of higher alcohols, polyoxyethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy
Propyl alcohol amidogen ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene glycerin ether or the one or more of dimethyl silicone polymer mix
Close object.Synergist is polyoxyethylene ether compound in the present embodiment, and the synergist in another embodiment of the invention is phosphoric acid
One or more mixtures of hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.This implementation
Wetting agent is fatty acid in example, and the wetting agent in another embodiment of the invention is naphthalene sulfonate and anion compound, alkane
One of base sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.This
Dispersing agent is nonionic in embodiment, and the dispersing agent in another embodiment of the invention is inorganic salts, polycarboxylate, phosphoric acid
One of ester, nonionic or comb-type structure anion or a variety of mixtures.Thickener in the present embodiment is that polysaccharide is compound
Object.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 26 DEG C in fermentation temperature, tank
Pressure is 0.03Mpa, ventilatory capacity 550m322h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 27min is centrifuged in the case where revolving speed is 2700rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 120MPa, handles 22min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 20 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 5 parts of synergist, 10 parts of dispersing agent, defoaming agent 0.2 are added thereto
Part, 3 parts of antifreeze, 10 parts of wetting agent, 0.6 part of thickener, 45 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.6, obtains yeast zinc blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 5
The application suspending agent one embodiment, the raw material in the embodiment includes following parts by weight, wherein soluble zinc-rich
50 parts of yeast, 15 parts of synergist, 25 parts of dispersing agent, 25 parts of wetting agent, 0.8 part of defoaming agent, 8 parts of antifreeze, 1.8 parts of thickener,
30 parts of water.Wherein the antifreeze in the present embodiment is salt water, and the antifreeze in another embodiment of the invention is ethylene glycol, first
One of alcohol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures.Defoaming agent in the present embodiment is poly- two
Methylsiloxane, the defoaming agent in another embodiment of the invention are silicone emulsion, the fatty acid ester compounded object of higher alcohols, polyoxy
Ethylene polyoxypropylene pentaerythrite ether, polyoxyethylene polyoxy propyl alcohol amidogen ether, polypropylene glycerol aether, polyoxyethylene polyoxypropylene are sweet
One or more mixtures of oily ether or dimethyl silicone polymer.Synergist is phosphoric acid hydrogen dimethylamino, citric acid three in the present embodiment
First and tamarind gum polymer, the synergist in another embodiment of the invention are phosphoric acid hydrogen dimethylamino, citric acid front three and sieve
One or more mixtures of shop sign in the form of a streamer xanthan polymer or polyoxyethylene ether compound.Wetting agent is aliphatic ester sulphur in the present embodiment
Hydrochlorate, the wetting agent in another embodiment of the invention are naphthalene sulfonate and anion compound, alkyl sulfate, sulfonic acid
One of salt, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixtures.Disperse in the present embodiment
Agent is comb-type structure anion, and the dispersing agent in another embodiment of the invention is inorganic salts, polycarboxylate, phosphate, non-
One of ion or comb-type structure anion or a variety of mixtures.Thickener in the present embodiment is polysaccharide compound.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 33 DEG C in fermentation temperature, tank
Pressure is 0.06Mpa, ventilatory capacity 650m328h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 33min is centrifuged in the case where revolving speed is 3300rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 180MPa, handles 28min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 50 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 15 parts of synergist, 25 parts of dispersing agent, defoaming agent 0.8 are added thereto
Part, 8 parts of antifreeze, 25 parts of wetting agent, 1.8 parts of thickener, 30 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 7.0, obtains yeast zinc blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 6
Another embodiment of the application suspending agent, the raw material in the embodiment includes following parts by weight, wherein soluble rich
30 parts of Zn-contained yeast, 12 parts of synergist, 17 parts of dispersing agent, 17 parts of wetting agent, 0.6 part of defoaming agent, 6 parts of antifreeze, thickener 1.2
Part, 20 parts of water.Wherein the antifreeze in the present embodiment is water-soluble amide, and the antifreeze in another embodiment of the invention is
One of ethylene glycol, methanol, ethyl alcohol, water-soluble amide, calcium chloride or salt water or a variety of mixtures.Defoaming in the present embodiment
Agent is the fatty acid ester compounded object of higher alcohols, and the defoaming agent in another embodiment of the invention is silicone emulsion, higher alcohols fat
Acid esters compound, polyoxyethylene polyoxy propyl alcohol amidogen ether, polypropylene glycerol aether, gathers polyoxyethylene polyoxypropylene pentaerythrite ether
One or more mixtures of oxypropylene polyoxyethylene glycerol ether or dimethyl silicone polymer.Synergist is phosphoric acid in the present embodiment
The mixture of hydrogen dimethylamino, citric acid front three and tamarind gum polymer and polyoxyethylene ether compound, another reality of the invention
Apply one kind that the synergist in example is phosphoric acid hydrogen dimethylamino, citric acid front three and tamarind gum polymer or polyoxyethylene ether compound
Or a variety of mixtures.Wetting agent is carboxylic acid soaps in the present embodiment, and the wetting agent in another embodiment of the invention is naphthalene sulphur
In hydrochlorate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate
One or more mixtures.Dispersing agent is inorganic salts and polycarboxylate mixture, another reality of the invention in the present embodiment
It is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixed for applying dispersing agent in example
Close object.Thickener in the present embodiment is polysaccharide compound.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc blade face fertilizer suspending agent making step is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 29 DEG C in fermentation temperature, tank
Pressure is 0.04Mpa, ventilatory capacity 590m3It is cultivated under conditions of/h for 24 hours, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 29min is centrifuged in the case where revolving speed is 2900rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 140MPa, handles 24min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 30 parts of the Zinc-rich saccharomyces cerevisiae is taken, and 12 parts of synergist, 17 parts of dispersing agent, defoaming agent 0.6 are added thereto
Part, 6 parts of antifreeze, 17 parts of wetting agent, 1.2 parts of thickener, 20 parts of water are uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.9, obtains yeast zinc blade face fertilizer suspending agent.
Sprinkling foliar fertilizer is carried out using application method in embodiment 1.
Embodiment 7
Herein described yeast zinc foliar fertilizer, one of production method and application can wet-milling embodiment, in the embodiment
Raw material includes following parts by weight, wherein 10 parts of soluble Zinc-rich saccharomyces cerevisiae, 1 part of wetting agent, 1 part of dispersing agent, 88 parts of anhydrous sodium sulphate.Its
Middle anhydrous sodium sulphate is sodium sulphate.Wetting agent is naphthalene sulfonate and anion compound, another implementation of the invention in the present embodiment
Wetting agent in example be naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate,
One of carboxylic acid soaps or phosphate or a variety of mixtures.Dispersing agent is inorganic salts, of the invention another in the present embodiment
Dispersing agent in embodiment is one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of
Mixture.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
It is shown in Figure 2, yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25 DEG C in fermentation temperature, tank
Pressure is 0.02Mpa, ventilatory capacity 500m320h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 25min is centrifuged in the case where revolving speed is 2500rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 100MPa, handles 20min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 10 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
1 part of wetting agent, 1 part of dispersing agent, 88 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Level-one mixing is carried out, primary mixes are obtained；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
The present embodiment application include:
In the present embodiment yeast zinc foliar fertilizer can wet-milling be applicable in various grains, melon fruits and vegetables, medicinal material, tealeaves, tobacco etc. work
Object (such as: rice, strawberry, lichee, longan, banana, apple, jujube, grape, peach, pears, watermelon, tealeaves, vegetables, medicinal material, rape,
Garlic, potato, soybean).
Yeast zinc in the present embodiment is produced during fermentating breeding to the effective metabolite of crops, is such as grown
Element, multivitamin and amino acid etc. can stimulate and adjust crop growth, improve increment.And farming can be induced
Peroxidase, polyphenol oxidase etc. participate in the defense reaction of crops in object, are conducive to the diseases prevention, disease-resistant of flowers and trees.
In the present embodiment yeast zinc foliar fertilizer can wet-milling application method are as follows:
By the yeast zinc foliar fertilizer obtained in the present embodiment can wet-milling be diluted with water；
Zinc-rich foliage fertilizer respectively after dilution is fitted into sprinkling tank, is sprayed with unmanned plane；Or it is manually or electrically sprayed
It spills；
Yeast zinc blade face fertilizer suspending agent can be sprayed in farming early growth period and growth medium；
, can be by spray head against based on the leaf back side to improve Foliage Absorption, uniformly sprinkling one time, not beat repeatedly back and forth；
If it is either progress at dusk before preferably in the morning 10 points of the outdoor outdoor cultivation of spring and autumn three quarters, such energy
Avoid the high temperature period, reduce evaporation, if winter booth vegetable melon and fruit plantation etc. can period at noon, because of winter temperature
It is low, and temperature of shed highest between 10 AM at 4 points in afternoon, it is most active that plant growth absorbs nutrient.
Yeast zinc foliar fertilizer of the invention has bioactivity height and easily absorption etc. by the way that inorganic zinc is converted to organic zinc
Advantage, the food containing yeast zinc have the characteristics that nontoxicity relative to the food containing chemical zinc and chelated zinc, and food is safer；This
Zn content in invention yeast zinc is high, and cooperation synergist keeps the fertilizer efficiency of yeast zinc foliar fertilizer of the present invention long, the wetting on leaf
Time is long, and penetrance is high, is easy to crops and absorbs Zn-ef ficiency, improves the Zn content of crops；It is strong to can promote crop root part
It is strong, root infection disease is reduced, the rhizome disease resistance of crops is enhanced；Promote the viscous of foliar fertilizer drop by the way that synergist is added
Attached power greatly prevents foliar fertilizer drop because environmental factor is low from blade face, evaporates, greatly reduce the wave of fertilizer
Take；Synergist is added, it is possible to reduce hard water ions generate the adverse effect such as antagonism to effective component, are conducive to the hair of fertilizer fertilizer
It waves；It can promote crop growth, improve the yield and percentage of fertile fruit of crops；The metabolism that can participate in protein in crops is made
With so as to improve the quality of crops；And the resistance of crops can be greatly improved.
Embodiment 8
Herein described yeast zinc foliar fertilizer, production method and application another can wet-milling embodiment, in the embodiment
Raw material include following parts by weight, wherein 60 parts of soluble Zinc-rich saccharomyces cerevisiae, 30 parts of wetting agent, 30 parts of dispersing agent, anhydrous sodium sulphate 30
Part.Wherein anhydrous sodium sulphate is sodium sulphate.Wetting agent is alkyl sulfate in the present embodiment, the profit in another embodiment of the invention
Humectant is naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps
Or one of phosphate or a variety of mixtures.Dispersing agent is polycarboxylate, another embodiment of the invention in the present embodiment
In dispersing agent be one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixing
Object.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 35 DEG C in fermentation temperature, tank
Pressure is 0.07Mpa, ventilatory capacity 700m330h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 35min is centrifuged in the case where revolving speed is 3500rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 200MPa, handles 30min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 60 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
30 parts of wetting agent, 30 parts of dispersing agent, 30 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Middle progress level-one mixing, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 9
The application yeast zinc foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight,
In, 35 parts of soluble Zinc-rich saccharomyces cerevisiae, 15 parts of wetting agent, 15 parts of dispersing agent, 35 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This
Wetting agent is sulfonate in embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound
One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing
Object.Dispersing agent is phosphate in the present embodiment, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate,
One of phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 2% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 30 DEG C in fermentation temperature, tank
Pressure is 0.05Mpa, ventilatory capacity 600m325h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3000rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 150MPa, handles 25min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 35 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
15 parts of wetting agent, 15 parts of dispersing agent, 35 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Middle progress level-one mixing, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 10
The application yeast zinc foliar fertilizer another can wet-milling embodiment, the raw material in the embodiment includes following parts by weight,
Wherein, 20 parts of soluble Zinc-rich saccharomyces cerevisiae, 10 parts of wetting agent, 10 parts of dispersing agent, 60 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.
Wetting agent is fatty acid in the present embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound
One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing
Object.Dispersing agent is nonionic in the present embodiment, the dispersing agent in another embodiment of the invention be inorganic salts, polycarboxylate,
One of phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 26 DEG C in fermentation temperature, tank
Pressure is 0.03Mpa, ventilatory capacity 55000m322h is cultivated under conditions of/h, obtains fermentation liquid；Contain in the fermentation medium
2% glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4、
0.1%MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 26min is centrifuged in the case where revolving speed is 2600rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 120MPa, handles 22min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 20 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
10 parts of wetting agent, 10 parts of dispersing agent, 60 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Middle progress level-one mixing, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 11
The application yeast zinc foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight,
In, 50 parts of soluble Zinc-rich saccharomyces cerevisiae, 25 parts of wetting agent, 25 parts of dispersing agent, 50 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This
Wetting agent is aliphatic ester sulfate in embodiment, the wetting agent in another embodiment of the invention be naphthalene sulfonate and yin from
One of sub- compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate are more
Kind mixture.Dispersing agent is comb-type structure anion in the present embodiment, and the dispersing agent in another embodiment of the invention is nothing
One of machine salt, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 33 DEG C in fermentation temperature, tank
Pressure is 0.06Mpa, ventilatory capacity 600m328h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 30min is centrifuged in the case where revolving speed is 3300rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 180MPa, handles 28min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 50 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
25 parts of wetting agent, 25 parts of dispersing agent, 50 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Middle progress level-one mixing, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
Embodiment 12
The application yeast zinc foliar fertilizer one can wet-milling embodiment, the raw material in the embodiment includes following parts by weight,
In, 36 parts of soluble Zinc-rich saccharomyces cerevisiae, 16 parts of wetting agent, 16 parts of dispersing agent, 32 parts of anhydrous sodium sulphate.Wherein anhydrous sodium sulphate is sodium sulphate.This
Wetting agent is carboxylic acid soaps in embodiment, and the wetting agent in another embodiment of the invention is that naphthalene sulfonate and anion are compound
One of object, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulfate, carboxylic acid soaps or phosphate or a variety of mixing
Object.Dispersing agent is the mixture of inorganic salts and polycarboxylate in the present embodiment, the dispersing agent in another embodiment of the invention
For one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or a variety of mixtures.
Zinc-rich saccharomyces cerevisiae derives from Shandong Bora benefit bioengineering limited liability company, is obtained using candida utili fermentation
?；Candida utili (Candida utilis) CUM is preserved in Chinese Typical Representative culture guarantor on April 19th, 2010
Hiding center, deposit number are CCTCC M 2010090.
Yeast zinc foliar fertilizer can wet-milling making step it is as follows:
Three-level activates candida utili CCTCC M 2010090, adds zinc sulfate in the medium in activation process
It is respectively 0.1mg/mL, 0.5mg/mL, 1mg/mL to ultimate density, obtains seed culture fluid；
According to 1% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 31 DEG C in fermentation temperature, tank
Pressure is 0.04Mpa, ventilatory capacity 620m326h is cultivated under conditions of/h, obtains fermentation liquid；Contain 2% in the fermentation medium
Glucose, 1%NH4H2PO4, 0.0001%CaCl2, 0.3% KH2PO4, 0.0001%MnSO4, 0.1%Na2HPO4, 0.1%
MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
The fermentation liquid 31min is centrifuged in the case where revolving speed is 3100rpm and is cleaned with clear water, is so repeated 3 times, will be centrifuged
Gained, which is deposited under 160MPa, handles 26min, obtains the soluble Zinc-rich saccharomyces cerevisiae.
Soluble 36 parts of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain zinc-rich ferment after air-drying
It is female；
16 parts of wetting agent, 16 parts of dispersing agent, 32 parts of anhydrous sodium sulphate are put into raw material mixing machine with Zinc-rich saccharomyces cerevisiae after described air-dry
Middle progress level-one mixing, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
By the available the second-order separation object separated in bag filter and the level-one isolate be sent into mixing machine into
The mixing of row second level, obtaining the yeast zinc foliar fertilizer can wet-milling.
Sprinkling foliar fertilizer is carried out using application method in embodiment 7.
As can be seen from the above embodiments beneficial effect existing for the application is:
The first, inorganic zinc by being converted to organic zinc by yeast zinc foliar fertilizer of the invention, with bioactivity height and easily
The advantages that absorption, the food containing yeast zinc have the characteristics that nontoxicity relative to the food containing chemical zinc and chelated zinc, and food is more pacified
Entirely.
The second, the Zn content in yeast zinc of the present invention is high, and cooperation synergist keeps the fertilizer efficiency of yeast zinc foliar fertilizer of the present invention long,
Wetting time on leaf is long, and penetrance is high, is easy to crops and absorbs Zn-ef ficiency, improves the Zn content of crops.
Zinc-rich saccharomyces cerevisiae in third, the present invention can promote crop root part stalwartness, reduce root infection disease, enhance farming
The rhizome disease resistance of object.
4th, the present invention promotes the adhesion strength of foliar fertilizer drop by the way that synergist is added, and greatly prevents blade face solution
Drop evaporates because environmental factor is low from blade face, greatly reduces the waste of fertilizer.
5th, the present invention is by being added synergist, it is possible to reduce hard water ions generate the unfavorable shadows such as antagonism to effective component
It rings, is conducive to the performance of fertilizer fertilizer.
6th, yeast zinc foliar fertilizer of the present invention can promote crop growth, improve the yield and percentage of fertile fruit of crops.
7th, yeast zinc foliar fertilizer of the present invention can participate in the metabolism of protein in crops, so as to improve farming
The quality of object.
8th, yeast zinc foliar fertilizer of the present invention can greatly improve the resistance of crops.
Although some specific embodiments of the invention are described in detail by example, the skill of this field
Art personnel it should be understood that example above merely to being illustrated, the range being not intended to be limiting of the invention.Although referring to before
Stating embodiment, invention is explained in detail, for those skilled in the art, still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features.It is all of the invention
Within spirit and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
The scope of the present invention is defined by the appended claims.
Claims (10)
1. a kind of yeast zinc foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Zinc-rich saccharomyces cerevisiae 10-
60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, 1-20 parts of synergist, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, thickener
0.1-3 parts, surplus be water.
2. yeast zinc foliar fertilizer according to claim 1, which is characterized in that the raw material including following parts by weight, wherein can
20-50 parts of dissolubility Zinc-rich saccharomyces cerevisiae, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, 5-15 parts of synergist, 0.3-0.8 parts of defoaming agent,
3-8 parts of antifreeze, 1-2 parts of thickener, surplus are water.
3. a kind of yeast zinc foliar fertilizer, which is characterized in that the raw material including following parts by weight, wherein soluble Zinc-rich saccharomyces cerevisiae 10-
60 parts, 1-30 parts of wetting agent, 1-30 parts of dispersing agent, surplus be anhydrous sodium sulphate.
4. yeast zinc foliar fertilizer according to claim 3, which is characterized in that the raw material including following parts by weight, wherein can
20-50 parts of dissolubility Zinc-rich saccharomyces cerevisiae, 10-20 parts of wetting agent, 10-20 parts of dispersing agent, surplus are anhydrous sodium sulphate.
5. yeast zinc foliar fertilizer according to claim 1 to 2, which is characterized in that the synergist include phosphoric acid hydrogen dimethylamino,
One or more mixtures of citric acid front three and tamarind gum polymer or polyoxyethylene ether compound.
6. yeast zinc foliar fertilizer described in -4 according to claim 1, which is characterized in that
The wetting agent includes: naphthalene sulfonate and anion compound, alkyl sulfate, sulfonate, fatty acid, aliphatic ester sulphur
One of hydrochlorate, carboxylic acid soaps or phosphate or a variety of mixtures；
The dispersing agent includes: one of inorganic salts, polycarboxylate, phosphate, nonionic or comb-type structure anion or more
Kind mixture；
The thickener includes: polysaccharide compound.
7. one kind yeast zinc foliar fertilizer production method as described in claim 1 to 6, which is characterized in that soluble rich including production
The step of Zn-contained yeast:
Three-level activates Ruan's Candida, and adding zinc sulfate in the medium in activation process is respectively 0.1mg/ to ultimate density
ML, 0.5mg/mL, 1mg/mL obtain seed culture fluid；
According to 1-3% inoculum concentration, the seed culture fluid is inoculated into fermentation medium, is 25-35 DEG C in fermentation temperature, tank
Pressure is 0.02-0.07Mpa, ventilatory capacity 500-700m320-30h is cultivated under conditions of/h, obtains fermentation liquid；The fermentation training
It supports and contains 2% glucose, 1%NH in base4H2PO4, 0.0001%CaCl2, 0.3%KH2PO4, 0.0001%MnSO4, 0.1%
Na2HPO4, 0.1%MgSO4·7H2O, 0.05% peptone, 0.0001%FeSO4, 0.15%ZnSO4；
It is centrifuged the fermentation liquid 25-35min in the case where revolving speed is 2500-3500rpm and is cleaned with clear water, so repeat 3 times, it will be from
It is deposited under 100-200MPa obtained by the heart and handles 20-30min, obtain the soluble Zinc-rich saccharomyces cerevisiae.
8. yeast zinc foliar fertilizer production method according to claim 7, which is characterized in that further include production yeast zinc blade face
The step of fertilizer suspending agent:
Soluble 10-60 parts of the Zinc-rich saccharomyces cerevisiae is taken, and synergist 1-20 parts, 1-30 parts of wetting agent, dispersing agent are added thereto
1-30 parts, 0.1-1 parts of defoaming agent, 1-10 parts of antifreeze, 0.1-3 parts of thickener, surplus be water, be uniformly mixed；
Calcium hydroxide is added thereto after mixing and adjusts pH to 6.5-7.2, obtains yeast zinc blade face fertilizer suspending agent.
9. yeast zinc foliar fertilizer production method according to claim 7, which is characterized in that further include production yeast zinc blade face
Fertilizer can wet-milling the step of:
Soluble 10-60 part of the Zinc-rich saccharomyces cerevisiae is taken, is placed under sterile ventilation condition and carries out air-dried, obtain air-drying zinc-rich ferment afterwards
It is female；
1-30 parts of wetting agents, 1-30 parts of dispersing agents, anhydrous sodium sulphate and the air-dried rear Zinc-rich saccharomyces cerevisiae are mixed, mixture is put into original
Expect to carry out level-one mixing in mixing machine, obtains primary mixes；
The primary mixes are sent into airslide disintegrating mill and carry out level-one crushing, obtain level-one crushed material；
The level-one crushed material is sent into cyclone separator and is separated, level-one isolate is obtained；
The available the second-order separation object separated in bag filter and the level-one isolate are sent into mixing machine and carry out two
Grade mixing, obtaining the yeast zinc foliar fertilizer can wet-milling.
10. a kind of application of the yeast zinc foliar fertilizer as described in claim 1 to 6.
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